Gene combination related to wheat flour color, dominant gene combination and application in breeding

Abstract

The invention provides a gene combination related to wheat flour color, a dominant gene combination and application in breeding, and belongs to the technical field of breeding. According to the invention, 12 genes influencing the color of the flour are screened by utilizing a 166 sample group, 9 dominant genes are screened, and the genes can be used as important genes of the color of the flour and have remarkable breeding utilization value. The invention proves that wheat varieties (lines) containing Psy-A1b, Pds-B1b, Lcye-A1b, Lcye-B1b, Lox-B1a, Ppo-A1b, Ppo-D1a, Pd-2D-GG / AG and non-1B / 1R translocation have advantages in flour color, the flour color is gradually improved along with the increase of the number of the wheat varieties (lines), and the wheat varieties containing the combination of at least six of the alleles have excellent flour color, can be used as excellent parents of high-brightness white flour wheat, and have broad application prospects. And an important material is provided for the cultivation of new wheat varieties.

Description

Technical Field

[0001] This invention belongs to the field of breeding technology, specifically relating to a gene combination, a dominant gene combination, and its use in breeding related to the color of wheat flour. Background Technology

[0002] Flour color is a sensory and market indicator for wheat quality evaluation, significantly impacting the flour milling industry, the flour-based food industry, and wheat production. Traditional Chinese wheat-based foods such as noodles, steamed buns, and dumplings are prized for their bright white color; therefore, developing wheat varieties with high-brightness white flour is a crucial goal in wheat quality breeding. Flour color is a complex quantitative trait, making marker-assisted selection (MAG) essential for improving breeding efficiency. Identifying superior allelic variations and clarifying breeding utilization effects are fundamental to MAG-assisted flour color improvement.

[0003] The color of flour and its products is mainly influenced by factors such as the content of yellow pigment (YPC) in the grains, the activity of oxidases such as lipoxygenase (LOX), polyphenol oxidase (PPO), and peroxidase (POD), as well as 1B / 1R translocations. In recent years, these factors have been highly valued in breeding practices, and significant progress has been made in the cloning of related genes and the development of functional markers, providing support for marker-assisted selection of flour color. Specifically, yellow pigment is the most important natural pigment in wheat grains, and its main component is carotenoids. Key enzymes in the carotenoid synthesis pathway, such as phytoene synthase (PSY), phytoenedesaturase (PDS), and ε-lycopene cyclase (LCYE), have been successively cloned, and molecular markers have been developed.

[0004] However, much of the existing work on detecting allelic variations in the color genes of flour has been limited to... Psy1 , Ppo1 and Lox1 There are a few genes, and comprehensive and systematic studies on related genes are limited. Furthermore, there are no reports on the comprehensive analysis of the effects of different allelic variations on the color of face. Summary of the Invention

[0005] This invention provides a gene combination related to wheat flour color, a dominant gene combination, and its use in breeding. The gene combination and dominant gene combination can be used to screen breeding parents and as breeding targets, and can be used for molecular marker-assisted breeding.

[0006] This invention provides a gene combination related to the color of wheat flour, including key enzyme genes of the carotenoid synthesis pathway, lipid oxidase genes, polyphenol oxidase genes, peroxidase genes, and 1B / 1R translocations. The key enzyme gene for the carotenoid synthesis pathway is selected from at least one of the following genes: Psy-A1 , Pds-B1 , Lcye-A1 and Lcye-B1 ; The lipoxygenase gene is Lox-B1 ; The polyphenol oxidase gene is selected from at least one of the following genes: Ppo-A1 , Ppo-B1 and Ppo-D1 ; The peroxidase gene is selected from at least one of the following genes: Pod-A1 , Pod-D1 and Pod-2D .

[0007] This invention provides a combination of dominant genes related to the color of wheat flour, including the following genes: Psy-A1 , Pds-B1 , Lcye-A1 , Lcye-B1 , Lox-B1 , Ppo-A1 , Ppo-D1 , Pod-2D Transposition with 1B / 1R.

[0008] This invention also provides the application of the above-mentioned gene combinations or the above-mentioned advantageous gene combinations in molecular marker-assisted breeding of wheat.

[0009] In a preferred embodiment of the present invention, when the target of the wheat marker-assisted breeding is the color of the wheat flour, wheat varieties containing at least 6 of the following alleles are considered superior varieties: Psy-A1b , Pds-B1b , Lcye-A1b , Lcye-B1b , Lox-B1a , Ppo-A1b , Ppo-D1a , Pod-2D-GG / AG Translocation between non-1B / 1R and 1B / 1R.

[0010] This invention also provides a method for screening wheat parents that exhibit dominant traits in flour color, including detecting wheat alleles, and selecting samples that contain a combination of at least 6 of the following alleles: Psy- A1b , Pds-B1b , Lcye-A1b , Lcye-B1b , Lox-B1a , Ppo-A1b ,Ppo-D1a , Pod-2D-GG / AG Translocation between non-1B / 1R and 1B / 1R.

[0011] In a preferred embodiment of the present invention, the detection includes a molecular labeling method.

[0012] The present invention also provides a breeding method for wheat with a flour color, comprising breeding with wheat parents that exhibit the dominant flour color trait selected by the above screening method as parents, and selecting offspring that aggregate more dominant genes than the parents; The advantageous genes include: Psy-A1 , Pds-B1 , Lcye-A1 , Lcye-B1 , Lox-B1 , Ppo-A1 , Ppo-D1 , Pod-2D Transposition with 1B / 1R.

[0013] In a preferred embodiment of the present invention, the offspring contain a combination of at least six alleles: Psy-A1b , Pds-B1b , Lcye-A1b , Lcye-B1b , Lox-B1a , Ppo-A1b , Ppo-D1a , Pod-2D-GG / AG Translocation between non-1B / 1R and 1B / 1R.

[0014] In a preferred embodiment of the present invention, the screening method for the offspring includes detection using molecular markers.

[0015] Beneficial effects: Based on 166 wheat varieties (lines), this invention screened 12 genes related to the color of bread using functional markers: Psy-A1 , Pds-B1 , Lcye-A1 , Lcye-B1 , Lox-B1 , Ppo-A1 , Ppo-B1 , Ppo-D1 , Pod-A1 , Pod-D1 , Pod-2D Translocation with 1B / 1R was used to determine the color of the face powder, including YPC. value, value, The study examined the color value and whiteness of face powder and identified nine genes that significantly influence the color of face powder, including: Psy-A1 and 1B / 1R Translocation significantly affects YPC, value, value, Value and whiteness; Lcye-B1Significantly affects YPC, value, Value and whiteness; Pds-B1 and Lox-B1 Significant impact value, Value and whiteness; Pod-2D Significant impact Value and whiteness; Lcye-A1 Significant impact value; Ppo-A1 and Ppo-D1 Significant impact Value. Genotypic analysis revealed that it contained... Psy-A1b , Pds-B1b , Lcye-A1b , Lcye-B1b , Lox- B1a , Ppo-A1b , Ppo-D1a , Pod-2D-GG / AG Non-1B / 1R translocations and other superior alleles are characterized by a bright white flour color in wheat varieties (lines) containing these superior alleles. Furthermore, as the number of superior alleles increases... value, The value and whiteness gradually increase, YPC and The value gradually decreases; the flour color of materials with 7-8 superior genes is the best. Therefore, the number of superior alleles can be used as the main indicator for evaluating parental vigor. Varieties with more than 7 superior alleles can be selected as parental materials for the breeding of high-brightness white wheat varieties, providing a basis and materials for molecular marker-assisted breeding of wheat flour color. Attached Figure Description

[0016] Figure 1 The distribution of superior allelic variations of nine important genes for flour color in 166 wheat varieties (lines); Figure 2 Regression analysis of the number of superior alleles and the color trait of wheat color in 166 wheat varieties (lines): (A) Correlation analysis of the number of superior alleles and yellow pigment content; (B) Correlation analysis of the number of superior alleles and the color trait of wheat color. Correlation analysis; (C) Number of superior alleles and Correlation analysis; (D) Number of superior alleles and Correlation analysis; (E) Correlation analysis between the number of superior alleles and whiteness; Figure 3 Regression analysis of the number of superior alleles and the color trait of wheat color in 140 wheat varieties (lines): (A) Correlation analysis of the number of superior alleles and yellow pigment content; (B) Correlation analysis of the number of superior alleles and the color trait of wheat color. Correlation analysis; (C) Number of superior alleles and Correlation analysis; (D) Number of superior alleles and Correlation analysis; (E) Correlation analysis between the number of superior alleles and whiteness. Detailed Implementation

[0017] This invention provides a gene combination related to the color of wheat flour, including key enzyme genes of the carotenoid synthesis pathway, lipid oxidase genes, polyphenol oxidase genes, peroxidase genes, and 1B / 1R translocations. The key enzyme gene for the carotenoid synthesis pathway is selected from at least one of the following genes: Psy-A1 , Pds-B1 , Lcye-A1 and Lcye-B1 ; The lipoxygenase gene is Lox-B1 ; The polyphenol oxidase gene is selected from at least one of the following genes: Ppo-A1 , Ppo-B1 and Ppo-D1 ; The peroxidase gene is selected from at least one of the following genes: Pod-A1 , Pod-D1 and Pod-2D .

[0018] In this embodiment of the invention, 166 wheat varieties (lines) were used to screen for functional markers related to YPC, value, value, Twelve genes related to color, such as value and whiteness, are derived from key enzyme genes in the carotenoid synthesis pathway, lipoxygenase genes, polyphenol oxidase genes, peroxidase genes, and 1B / 1R translocations. Psy-A1 (TraesCS7A02G557300) Pds-B1 (TraesCS4B02G300100) Lcye-A1 (TraesCS3A02G208800) Lcye-B1 (TraesCS3B02G239200) Lox-B1 (TraesCS4B02G037900) Ppo-A1 (TraesCS2A02G468200) ​ (TraesCS2B02G491000) ​ (TraesCS2D02G468200) ​ (TraesCS3A02G510600) ​ (TraesCS7D02G066400) ​(TraesCS2D02G583700) and 1B / 1R translocations. Nine genes that significantly affect the color of face powder were screened out, including: ​ A1 and ​ Translocation significantly affects YPC, value, value, Value and whiteness; ​ Significantly affects YPC, value, Value and whiteness; ​ and ​ Significant impact value, Value and whiteness; ​ Significant impact Value and whiteness; ​ Significant impact value; ​ and ​ Significant impact value.

[0019] This invention provides a combination of dominant genes related to the color of wheat flour, including the following genes: ​ , ​ , ​ , ​ , ​ , ​ , ​ , ​ Transposition with 1B / 1R.

[0020] This invention, through allele analysis of 166 wheat varieties (lines), discovered that they contain... ​ , ​ ​ , ​ , ​ , ​ , ​ , ​ , ​ Non-1B / 1R translocations and other superior alleles are specifically manifested in wheat varieties (lines) containing superior alleles, which exhibit a bright white flour color. Furthermore, the more alleles aggregated, the better the wheat flour color. In this invention, the aggregation of more than six genes shows a highly significant difference in flour color compared to other varieties (lines).

[0021] This invention also provides the application of the above-mentioned gene combinations or the above-mentioned advantageous gene combinations in molecular marker-assisted breeding of wheat.

[0022] When the target of the marker-assisted breeding of wheat in this invention is the color of the wheat flour, wheat varieties containing at least 6 of the following alleles are considered superior varieties: ​ , ​ , ​ , ​ , ​ , ​ , ​ , ​ Translocation between non-1B / 1R and 1B / 1R.

[0023] This invention also provides a method for screening wheat parents exhibiting dominant traits in flour color, comprising using the above-mentioned primer set for detection, and selecting samples that contain at least 6 of the following alleles: ​ , ​ , ​ , ​ , ​ , ​ , ​ , ​ Translocation between non-1B / 1R and 1B / 1R.

[0024] The detection method described in this invention includes detection using molecular markers, wherein the molecular markers include YP7A, POD-3A2, YP4B-1, YP4B-2, YP3B-1, PPO16, PPO18, PPO29, LOX16, LOX18, POD-3A1, e-LCY3A-3, POD-7D1, F-8, POD-7D6, H2O, and Excalibur_c95720_329-KASP.

[0025] Table 1. Molecular marker detection genes and references

[0026] The present invention also provides a breeding method for wheat with a flour color, comprising breeding with wheat parents that exhibit the dominant flour color trait selected by the above screening method as parents, and selecting offspring that aggregate more dominant genes than the parents; The advantageous genes include: ​ , Pds-B1 , Lcye-A1 , Lcye-B1 , Lox-B1 , Ppo-A1 , Ppo-D1 , Pod-2D Transposition with 1B / 1R.

[0027] The offspring screening method of the present invention includes detecting wheat alleles, and after the detection, the offspring contain a combination of at least 6 of the following alleles: Psy-A1b , Pds-B1b ,Lcye-A1b , Lcye-B1b , Lox-B1a , Ppo-A1b , Ppo-D1a , Pod-2D-GG / AG Translocation between non-1B / 1R and 1B / 1R.

[0028] To further illustrate the present invention, the following detailed description, in conjunction with embodiments, of a gene combination, a dominant gene combination, and its use in breeding related to wheat flour color, provided by the present invention, should not be construed as limiting the scope of protection of the present invention.

[0029] Unless otherwise specified, all materials used in the embodiments of this invention are from conventional sources in the art, and all experimental methods used are conventional methods.

[0030] The 166 wheat varieties (lines) used in this invention were provided by the National Wheat Genetic Improvement Center of the Chinese Academy of Agricultural Sciences. The experimental materials were planted in Jinan and Dezhou in the 2019-2020 and 2020-2021 seasons, respectively. A randomized block design was used, with 3 rows per plot, 2 m row length, 50 grains per row, 25 cm row spacing, and two replicates. Standard field management was implemented, and the grains were manually threshed after harvest.

[0031] Table 2 Genotypic information of 166 tested wheat varieties (lines)

[0032] Table 3. Flour color and genotypic information of 166 tested wheat varieties (lines).

[0033] In this embodiment of the invention, molecular marker detection of color-related genes in wheat flour was performed: three seeds were selected from each material, and wheat genomic DNA was extracted using the SDS method for gene locus detection. Molecular markers such as YP7A, LOX16, and PPO18 were used to detect gene loci in 166 wheat materials. Psy-A1 , Lox-B1 andPpo-A1 Twelve genes related to the color of the pasta were tested. Pod-A1 and Pod- D1 The locus genotype data are cited from previously published articles (Wei JX, Geng HW, Zhang Y, Liu JD, Wen WE, Zhang Y, Xia XC, Chen XM, He ZH. Mapping quantitative trait loci for peroxidase activity and developing gene-specific markers for...). TaPod-A1 onwheat chromosome 3AL. Theoretical and Applied Genetics, 2015, 128(10): 2067-2076.; Geng HW, Shi J, Fuerst EP, Wei JX, Morris C F. Physical mapping of peroxidase genes and development of functional markers for TaPod-D1 on breadwheat chromosome 7D. Frontiers in Plant Science, 2019, 10: 523.).

[0034] The specific method for determining the color properties of flour in this embodiment of the invention is as follows: Grain hardness was determined using a single-grain cereal characteristic tester (SKCS4100, Perten, Sweden). Moisture content was determined using a near-infrared analyzer (Foss-Tecator 1241, Foss, Högänas, Sweden). The wheat was moistened using AACC 26-95 method, and milled using a Quandrit Junior experimental mill (Branbender, Germany) according to AACC 26-50 method (AACC. 2000. Approved methods of the American Association of Cereal Chemists, 10th edn. American Association of Cereal Chemists, Incorporated, St Paul, Minnesota.). The flour was stored at 4°C.

[0035] The color of face powder was measured using a Minolta CR-310 colorimeter (Minolta Camera Co. Ltd. Japan). value, value, Value colorimetric system, in which The value represents brightness (0 represents black, and the larger the value, the whiter it is). The value represents the red-green color (negative values ​​represent green, and the larger the value, the greener it is); The value represents the yellow-blue tint (positive values ​​indicate yellow, with higher values ​​indicating a more yellow hue). Each sample was repeated three times, and the average value was its phenotypic value. Flour whiteness was calculated using the Hunter's whiteness formula, which is as follows: , Formula I.

[0036] YPC was determined using a high-throughput method for detecting yellow pigment content. 0.5 g of flour was weighed, and 2.5 mL of water-saturated n-butanol was added. The mixture was extracted by shaking for 30 min; centrifuged at 5000 rpm for 10 min; and the absorbance (A) at 436.5 nm was measured using a SpectraMax Plus 384 microplate reader (MolecularDevices, LLC, USA). Yellow pigment content = A × 30.1, in μg·g. -1 Each sample was repeated 3 times, and the mean value was used as the sample YPC.

[0037] Example 1 1. The effect of different allelic variants on the color of face pink The significance analysis of the differences in surface color trait among different genotypes at the same gene locus is shown in Table 4. Psy-A1 Genes and 1B / 1R translocations significantly affect YPC, value, value, Value and whiteness, containing Psy-A1b Allelic variation and non-1B / 1R translocation materials value, Value and whiteness are significantly higher than Psy-A1a Allelic variation and 1B / 1R translocation materials ( P <0.05), while YPC and The value is significantly lower than the latter ( P <0.01).

[0038] Lcye-B1 Genes significantly influence YPC, value, Value and whiteness, containing alleles Lcye-B1b materials The values ​​(-0.82 vs -0.96) and whiteness (87.04 vs 86.32) were significantly higher than those containing alleles.Lcye-B1a Material( P <0.05), while YPC (1.12 vs 1.30 μg·g) -1 )and The value (8.55 vs 9.39) is significantly lower than the latter ( P <0.01).

[0039] Pds-B1 and Lox-B1 Significant influence of genes value, Value and whiteness, containing Pds-B1b and Lox-B1a Allele materials The value and whiteness are significantly higher than those containing Pds-B1a and Lox-B1b Allele materials, and The value is significantly lower than the latter ( P <0.05).

[0040] Pod-2D Significant influence of genes Value and whiteness, Pod-2D-AA , Pod-2D-AG and Pod-2D-GG Inter-alloy variation materials The difference in value and whiteness reached a significant level. P <0.05).

[0041] Lcye-A1 Significant influence of genes Values ​​containing allelic variations Lcye-A1b materials The value was significantly higher than that containing allelic variations. Lcye-A1a Materials (90.33 vs 89.13, P <0.05).

[0042] Ppo-A1 and Ppo-D1 Significant influence of genes Value, containing Ppo-A1b and Pod-D1a Allele materials The value is significantly higher than that containing Ppo-A1a and Pod-D1b Allele materials ( P <0.05). And... Ppo-B1 , Pod-A1 and Pod-D1 There was no significant difference in surface color among different genotypes at the locus.

[0043] In summary, Psy-A1 , Pds-B1 , Lcye-A1 , Lcye-B1 ,Lox-B1 , Ppo-A1 , Ppo-D1 , Pod-2D The 1B / 1R translocation significantly affects the color of face powder, making it an important gene for face powder color and possessing great potential for breeding applications. It contains alleles. Psy- A1b , Pds-B1b , Lcye-A1b , Lcye-B1b , Lox-B1a , Ppo-A1b , Ppo-D1a , Pod-2D-GG / AG Wheat varieties (lines) that are not 1B / 1R translocations have a bright white flour color (high... value, Value and whiteness or low YPC and The above genotypes were named superior alleles based on their values.

[0044] Table 4. Effects of different allelic variants on face color

[0045] a, b, AA, GG, and AG represent the allelic variations of the corresponding genes; Y indicates the presence of a 1B / 1R translocation, and N indicates a non-1B / 1R translocation. and These indicate that the difference in surface color between two alleles at the same gene locus reached a significant level. P <0.05) and highly significant ( P <0.01) level.

[0046] 3. Superior allele aggregation Among the 166 tested wheat varieties (lines), the superior allelic variations of nine important flour color genes showed a normal distribution. Varieties with four and five superior alleles were the most numerous, at 44 and 37 varieties respectively, accounting for 26.51% and 22.29% of the total samples. Figure 1 (A). Only Zhengyin 1 and Zimai 12 aggregated 9 superior alleles, with a frequency of 1.20%; 6 wheat varieties, including Wanmai 19, Wanmai 50 and Wanmai 52, aggregated 8 superior alleles, with a frequency of 3.61%; 14 wheat varieties, including Aifeng 3, Fengchan 3 and Fu 936, aggregated 7 superior alleles, with a frequency of 8.43% (Table 5).

[0047] Table 5. Superior germplasm containing 7 or more superior alleles

[0048] 4. Analysis of the additive effects of important genes for face color Regression analysis was performed on the number of superior alleles of the above nine important genes for face color and face color. The results showed that the number of superior alleles was related to YPC and The values ​​are significantly negatively correlated ( Figure 2 China A and Figure 2 (D), and with value, Value and whiteness are significantly positively correlated ( Figure 2 B, Figure 2 C and Figure 2 (E). With the increase in the number of superior alleles, YPC ( R 2 = 0.84, P <0.001) and value( R 2 = 0.93, P <0.001) gradually decreased, while value( R 2 = 0.83, P <0.05), value( R 2 = 0.86, P <0.001) and whiteness ( R 2 =0.93, P <0.001) gradually increases ( Figure 2 (Table 6).

[0049] Table 6. Effects of the number of superior gene aggregations on the color of wheat flour in wheat varieties (lines).

[0050] Since only two wheat varieties aggregated nine superior allelic variations, multiple comparison analysis was not performed; different letters after the data in the same column indicate that the differences in surface color among wheat varieties aggregating different superior genes reached a significant level. P <0.05).

[0051] Multiple comparative analysis of the effect of the number of superior allele aggregations on wheat color further showed that wheat varieties (lines) YPC with one superior allele aggregation (1.95 μg·g) had a higher color color. -1 )and The material YPC (0.85 and 0.83 μg·g) had the highest value (11.65) and aggregated 7-8 superior genes. -1 The b-values ​​(7.03 and 6.68) were the lowest, and there were significant differences compared to materials that aggregated 1-4 superior genes. P <0.05); Conversely, materials that aggregate 7-8 superior genes Values ​​(90.95 and 91.23) The values ​​(-0.64 and -0.67) and whiteness were the highest (88.50 and 88.95), and significantly higher than materials with 1-4 superior genes (Table 6). P <0.05). Since only two wheat varieties, Zhengyin 1 and Zimai 12, aggregated 9 superior genes, multiple comparison analysis was not performed on them, but their flour color index was similar to that of materials that aggregated 7-8 superior genes (Table 6).

[0052] In summary, the number of superior allele aggregations is closely related to the color of face powder; as the number of superior genes increases, YPC ( R 2 = 0.84, P <0.001) and value( R 2 = 0.93, P <0.001) gradually decreased, while value( R 2 = 0.83, P <0.05), value( R 2 = 0.86, P <0.001) and whiteness ( R 2 = 0.93, P <0.001) gradually increases; the material with 7-8 superior genes has the best surface color, that is value, The highest values ​​for whiteness and YPC and b values ​​indicate that using gene-specific molecular markers to precisely aggregate multiple superior allelic variations is an efficient method for improving flour color. This invention screened 22 wheat varieties, including Zhengyin 1, Zimai 12, and Wanmai 19, which aggregated more than 7 superior allelic variations. These can be used as priority parents for breeding high-brightness white flour wheat to accelerate the aggregation of superior genes, rapidly increase the frequency of superior genes, significantly improve breeding efficiency, and achieve precise and efficient improvement of flour color.

[0053] Example 2 The 140 wheat varieties (lines) shown in Table 7 were tested using the same method as in Example 1 (Tables 7 and 8). The results showed that the number of superior allele aggregations was closely related to the color of the flour; as the number of superior genes increased, YPC (… R 2 = 0.96, P <0.0001) and value( R 2= 0.83, P <0.01) gradually decreased, while value( R 2 = 0.92, P <0.001) value( R 2 = 0.82, P <0.01) and whiteness ( R 2 = 0.88, P <0.01) gradually increases ( Figure 3 Materials containing six or more superior genes have the best powder color. value, The values ​​and whiteness are the highest, while the YPC and b values ​​are the lowest (Table 9).

[0054] Table 7 Genotypic information of 140 wheat varieties (lines)

[0055] Table 8. Flour color and genotype information of 140 wheat varieties (lines)

[0056] Table 9. Effects of the number of superior gene aggregations on the color of wheat flour in wheat varieties (lines).

[0057] Only one wheat variety aggregated one superior allelic variant, and no multiple comparison analysis was performed; different letters after the data in the same column indicate that the differences in surface color among wheat varieties aggregating different superior genes reached a significant level. P <0.05).

[0058] Although the above embodiments have provided a detailed description of the present invention, they are only some embodiments of the present invention, and not all embodiments. People can obtain other embodiments based on these embodiments without creative effort, and these embodiments all fall within the protection scope of the present invention.

Claims

1. A gene combination associated with the color of wheat flour, characterized in that, This includes key enzyme genes in the carotenoid synthesis pathway, lipoxygenase genes, polyphenol oxidase genes, peroxidase genes, and 1B / 1R translocations; The key enzyme gene for the carotenoid synthesis pathway is selected from at least one of the following genes: Psy-A1 , Pds-B1 , Lcye- A1 and Lcye-B1 ; The lipoxygenase gene is Lox-B1 ; The polyphenol oxidase gene is selected from at least one of the following genes: Ppo-A1 , Ppo-B1 and Ppo-D1 ; The peroxidase gene is selected from at least one of the following genes: Pod-A1 , Pod-D1 and Pod-2D .

2. A combination of dominant genes related to the color of wheat flour, characterized in that, Including the following genes: Psy-A1 , Pds-B1 , Lcye-A1 , Lcye-B1 , Lox-B1 , Ppo-A1 , Ppo-D1 , Pod-2D Transposition with 1B / 1R.

3. The application of the gene combination of claim 1 or the dominant gene combination of claim 2 in molecular marker-assisted breeding of wheat.

4. The application according to claim 3, characterized in that, When the goal of the marker-assisted breeding of wheat is to improve the color of the wheat, wheat varieties containing at least 6 of the following alleles are considered dominant varieties: Psy-A1b , Pds-B1b , Lcye-A1b , Lcye-B1b , Lox-B1a , Ppo-A1b , Ppo-D1a , Pod-2D-GG / AG Translocation between non-1B / 1R and 1B / 1R.

5. A method for screening wheat parents whose flour color exhibits dominant traits, characterized in that, Allele testing was conducted on wheat varieties, and samples were selected if they contained at least six of the following alleles: Psy-A1b , Pds-B1b , Lcye-A1b , Lcye-B1b , Lox-B1a , Ppo-A1b , Ppo-D1a , Pod-2D-GG / AG Translocation between non-1B / 1R and 1B / 1R.

6. The screening method according to claim 5, characterized in that, The detection includes methods using molecular markers.

7. A breeding method for wheat with a color related to flour, characterized in that, This includes breeding with wheat parents that exhibit the dominant color trait of flour as selected by the screening method described in claim 5 or 6, and selecting offspring that aggregate more dominant genes than the parents; The advantageous genes include: Psy-A1 , Pds-B1 , Lcye-A1 , Lcye-B1 , Lox-B1 , Ppo-A1 , Ppo-D1 , Pod- 2D Transposition with 1B / 1R.

8. The breeding method according to claim 7, characterized in that, The offspring contain a combination of at least six of the following alleles: Psy-A1b , Pds-B1b , Lcye-A1b , Lcye-B1b , Lox-B1a , Ppo-A1b , Ppo-D1a , Pod-2D-GG / AG Translocation between non-1B / 1R and 1B / 1R.

9. The breeding method according to claim 8, characterized in that, The screening method for the offspring includes detection using molecular markers.

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