Seed tank automatic feeding device and feeding method

Abstract

The invention relates to the technical field of seed tank feeding equipment and methods, in particular to an automatic seed tank feeding device and method. The sample adding mechanism is used for automatically adding culture medium raw materials; the monitoring mechanism is used for monitoring the liquid level of the tank body; the sample injection mechanism is connected with a water tank, and the sample injection mechanism is used for communication among the water tank, the sample adding mechanism, the tank body and the monitoring mechanism. By arranging the sample adding mechanism and the sample injection mechanism, stable and uniform conveying of solid or liquid raw materials is achieved, flow is accurately controlled in cooperation with the pump machine, it is guaranteed that the culture medium proportion is accurate, the consistency of the culture process is improved, automatic sample adding is achieved through the pump machine, and the labor cost can be effectively reduced. Meanwhile, the sampling mechanism is matched with the monitoring mechanism, so that real-time and synchronous simulation monitoring of the liquid level of the tank body can be realized.

Description

Technical Field

[0001] This invention relates to the technical field of seed tank feeding equipment and methods, specifically to an automatic seed tank feeding device and feeding method. Background Technology

[0002] Seed tanks are key pieces of equipment in industrial fermentation and bioculture processes, primarily used for the scale-up cultivation of microorganisms. In traditional seed tank cultivation, the feeding process typically relies on manual operation or semi-automated equipment.

[0003] Traditional seed tank filling primarily uses funnels and water buckets, with manual filling being the main method. This method is time-consuming and labor-intensive, especially at high production volumes, consuming significant labor costs. Furthermore, material loss during filling can easily occur, leading to variations in the formula within the tank and affecting the growth of the inoculum. Additionally, the liquid level in the tank often relies on manual observation or simple level gauges, which are easily affected by factors such as foam and air bubbles, resulting in inaccurate volume determination and poor batch-to-batch repeatability.

[0004] In view of this, we propose an automatic seed tank feeding device. Summary of the Invention

[0005] The purpose of this invention is to provide an automatic seed tank feeding device and feeding method, which solves the problems mentioned in the background art.

[0006] To achieve the above objectives, the present invention provides the following technical solution: An automatic seed tank feeding device includes a tank body; A sample dispensing mechanism for the automatic addition of culture medium raw materials; A monitoring agency used to monitor the liquid level in the tank; The sample injection mechanism is connected to a water tank and is used for communication between the water tank, the sample addition mechanism, the tank body, and the monitoring mechanism.

[0007] Preferably, the injection mechanism includes a main pipe, one end of which is fixedly connected to a water tank, a feed pump is fixedly installed on the main pipe, and a feed branch pipe is provided between the main pipe and the tank body.

[0008] Preferably, a monitoring branch pipe is fixedly installed on the main pipe, and a monitoring pump is fixedly installed on the monitoring branch pipe. The monitoring branch pipe is located between the feed pump and the water tank.

[0009] Preferably, the monitoring mechanism includes a protective frame, which is fixedly installed on the tank body. A display tube is fixedly installed inside the protective frame. A piston one is slidably installed inside the display tube near the bottom end, and a piston two is slidably installed inside the display tube near the top end. A monitoring injection tube is provided between the feed branch pipe and the display tube.

[0010] Preferably, a first contact piece is fixedly mounted on the first piston, and a second contact piece is fixedly mounted on the second piston, and the first contact piece and the second contact piece are electrically connected.

[0011] Preferably, a ball screw is fixedly installed at the top of the piston two, the ball screw is slidably connected to the protective frame and the display cylinder, an adjusting vane is threadedly connected to the surface of the ball screw, a limit rod is fixedly installed at the top of the ball screw through a bracket, the limit rod is slidably connected to the protective frame, and the adjusting vane is rotatably connected to the protective frame.

[0012] Preferably, the sampling mechanism includes a sampling container, a sampling tube is fixedly installed at the bottom of the sampling container, the sampling tube is fixedly connected to the main pipe, an auger is provided inside the sampling tube, and multiple venturi tubes are provided in the main pipe near the sampling tube.

[0013] Preferably, a crossbeam is fixedly installed at the top of the sample addition tank, a motor is fixedly installed on the crossbeam, a rotating shaft is fixedly connected to the output shaft of the motor, a rotating blade is fixedly installed on the surface of the rotating shaft, and the rotating shaft is fixedly connected to the auger.

[0014] Preferably, a rinsing pipe is fixedly installed on the water tank, a rinsing pump is fixedly installed on the rinsing pipe, and the other end of the rinsing pipe is positioned above the sample addition tank.

[0015] This invention also provides an automatic seed tank feeding method, comprising the following steps: S1. Adding materials: Add the raw materials into the sample feeding container; S2. Adjustment: According to the required liquid level in the tank, rotate the adjusting vane to drive the ball screw and piston II to move down to the preset position in the display cylinder; S3. Feeding: Start the feed pump and motor. The motor drives the shaft to rotate the auger, which pushes the raw material in the sample tank evenly and stably to the main pipe, and then flows into the tank through the feed branch pipe. S4. Diverting the flow: Start the feed pump and start the monitoring pump at the same time to ensure that the flow ratio on the main pipe and the monitoring branch pipe is constant. The liquid in the monitoring branch pipe is delivered to the display cylinder through the monitoring injection tube. S5. Rinse: Start the rinsing pump and rinse the sample tank through the rinsing pipe to ensure that all the raw materials enter the tank. S6. Volume control: As the liquid continues to enter the display cylinder, piston one gradually moves upward until contact piece one contacts contact piece two and generates an electrical signal. After receiving the signal, the PLC controller synchronously controls the valves on the feed branch pipe and the monitoring sample inlet pipe to stop the feed, thereby achieving automatic volume control and stopping the feeding. S7. Circulation: The feed pump and monitoring pump operate continuously to maintain liquid flow in the pipeline and prevent the growth of miscellaneous bacteria.

[0016] By employing the above technical solution, the present invention provides an automatic seed tank feeding device and feeding method that has at least the following beneficial effects: (1) This invention achieves stable and uniform delivery of solid or liquid raw materials by setting up a sample addition mechanism and a sample injection mechanism. With the precise control of the flow rate by the pump, the accurate ratio of the culture medium is ensured, and the consistency of the culture process is improved. Automatic sample addition by the pump can effectively reduce labor costs. At the same time, the sample injection mechanism, together with the monitoring mechanism, can realize real-time and synchronous simulation monitoring of the liquid level in the tank.

[0017] (2) By setting up a monitoring mechanism, the piston structure of the monitoring mechanism can eliminate bubble interference, and the contact plate trigger signal realizes automatic volume control, thereby improving the accuracy and reliability of liquid level control. The adjustable position of piston two allows for accurate control of the liquid level height at constant volume, improving applicability. Attached Figure Description

[0018] The accompanying drawings, which are provided to further illustrate the invention, constitute a part of this application: Figure 1 This is a schematic diagram of the structure of the present invention; Figure 2 This is a schematic diagram of the feeding structure of the present invention; Figure 3 This is a schematic diagram of the sample feeding mechanism of the present invention; Figure 4 This is a schematic cross-sectional view of the sample feeding mechanism of the present invention; Figure 5 This is a schematic cross-sectional view of the connection between the sample feeding mechanism and the main pipe of the present invention. Figure 6 This is a schematic diagram of the monitoring mechanism of the present invention; Figure 7 This is a schematic cross-sectional view of the monitoring mechanism of the present invention; Figure 8 This is a schematic diagram of the partially disassembled structure of the monitoring mechanism of the present invention.

[0019] In the diagram: 1. Tank body; 2. Sampling mechanism; 3. Monitoring mechanism; 4. Water tank; 5. Sampling injection mechanism; 21. Sample loading vessel; 22. Base; 23. Sample outlet tube; 24. Crossbeam; 25. Rotating shaft; 26. Rotating blade; 27. Screwdriver; 31. Protective frame; 32. Display tube; 33. Piston 1; 34. Contact plate 1; 35. Piston 2; 36. Contact plate 2; 37. Adjusting vane; 38. Ball screw; 39. Limiting rod; 51. Flushing tube; 52. Flushing pump; 53. Main pipe; 531. Venturi tube; 5311. Contraction section; 5312. Throat; 5313. Expansion section; 54. Feed pump; 55. Monitoring branch pipe; 56. Monitoring pump; 57. Feed branch pipe; 58. Monitoring sample inlet tube. Detailed Implementation

[0020] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0021] Please see Figures 1-8 An automatic feeding device for a seed tank, comprising a tank body 1, is a key piece of equipment for industrial fermentation culture of microorganisms, used for the scale-up cultivation of microorganisms. A sample feeding mechanism 2 is connected to the tank body 1 for adding raw materials to the culture medium.

[0022] Please see Figure 3 and Figure 4 The sample dispensing mechanism 2 includes a base 22, on which a sample dispensing container 21 is fixedly mounted. The sample dispensing container 21 has a frustum-shaped structure with an open top. The inclined surface of its inner surface allows the raw materials of the culture medium to automatically gather towards the bottom of the container under gravity, facilitating the dispensing process. A crossbeam 24 is fixedly mounted at the top of the sample dispensing container 21, and a motor is fixedly mounted on the crossbeam 24. A rotating shaft 25 is fixedly connected to the output shaft of the motor. The crossbeam 24 provides a stable working environment for the motor, and the motor's operation drives the rotating shaft 25 to rotate. A rotating blade 26 is fixedly mounted on the surface of the rotating shaft 25. The rotation of the rotating shaft 25 drives the rotating blade 26 to rotate synchronously, and the rotating blade 26 contacts the inner surface of the sample dispensing container 21. This allows the rotating blade 26 to agitate the material on the surface of the sample dispensing container 21, preventing the material from adhering to the inner surface of the container.

[0023] In this embodiment, a sample outlet pipe 23 is fixedly installed at the bottom of the sample loading vessel 21 for discharging the culture medium raw materials. An auger 27 is fixedly installed at the bottom of the rotating shaft 25. The auger 27 is disposed inside the sample outlet pipe 23 and contacts the inner wall of the sample outlet pipe 23, so that the auger 27 can transport the culture medium raw materials.

[0024] Please see Figure 1 The tank 1 is equipped with a monitoring mechanism 3, which is used to monitor the liquid level in the tank 1 and control the constant volume liquid level.

[0025] Please see Figure 6 and Figure 7The monitoring mechanism 3 includes a protective frame 31, which is fixedly installed on the tank body 1. A display cylinder 32 is fixedly installed inside the protective frame 31. The display cylinder 32 is made of transparent material, allowing direct display of the liquid level within the cylinder. A scale is also provided on the surface of the display cylinder 32, making the liquid level display more intuitive. A piston 33 is slidably installed near the bottom of the display cylinder 32. The piston 33 is a sealed piston, preventing liquid from passing through it and entering above it. The movement of the piston 33 is solely driven by the liquid, and its bottom surface is rough to break any air bubbles. This effectively prevents air bubbles from affecting the liquid level and avoids misjudgments.

[0026] In this embodiment, a piston 35 is slidably mounted near the top of the display cylinder 32. A through hole is provided through the piston 35 to allow the display cylinder 32 to communicate with the outside, preventing excessive air pressure inside the display cylinder 32 from preventing liquid from being delivered into the display cylinder 32. A contact piece 34 is fixedly mounted on the piston 33, and a contact piece 36 is fixedly mounted on the piston 35. The contact piece 34 and the contact piece 36 are electrically connected. An electrical signal is generated through the contact between the contact piece 34 and the contact piece 36, which, under PLC control, stops the liquid from entering the display cylinder 32 and the tank 1.

[0027] Please see Figure 8 A ball screw 38 is fixedly mounted on the top of piston 35. The ball screw 38 is slidably connected to the protective frame 31 and the display cylinder 32. An adjusting vane 37 is threaded onto the surface of the ball screw 38. A limit rod 39 is fixedly mounted on the top of the ball screw 38 via a bracket. The limit rod 39 is slidably connected to the protective frame 31, and the adjusting vane 37 is rotatably connected to the protective frame 31. When the adjusting vane 37 is rotated, it rotates along the protective frame 31. Due to the threaded connection between the adjusting vane 37 and the ball screw 38, and the slidable connection between the limit rod 39 and the protective frame 31, the ball screw 38 can only move up and down along the adjusting vane 37. The displacement of the ball screw 38 causes the piston 35 to move, thereby adjusting the position of piston 35.

[0028] It should be noted that the ball screw 38 is slidably connected to the protective frame 31 and the display cylinder 32 to avoid affecting the operation of the ball screw 38. The ball screw 38 has a hollow internal structure for easy wiring.

[0029] Please see Figure 1 A sampling mechanism 5 is provided between the tank body 1 and the sample addition tank 21. The sampling mechanism 5 is connected to the water tank 4. The sampling mechanism 5 is used for communication between the water tank 4, the sampling mechanism 2, the tank body 1, and the monitoring mechanism 3.

[0030] Please see Figure 2The sample injection mechanism 5 includes a main pipe 53, one end of which is fixedly connected to the water tank 4. A feed pump 54, which is a peristaltic pump, is fixedly installed on the main pipe 53 and can control the flow rate and volume of the liquid. A feed branch pipe 57 is provided between the main pipe 53 and the tank 1, and a three-way valve is provided between the feed branch pipe 57 and the main pipe 53. The feed pump 54 can drive the liquid in the main pipe 53 into the tank 1.

[0031] Please see Figure 5 The sample outlet tube 23 is fixedly connected to the main tube 53. The main tube 53 is provided with multiple Venturi tubes 531 near the sample outlet tube 23. Each Venturi tube 531 includes a constriction section 5311, a throat 5312, and an expansion section 5313. The liquid gradually accelerates as it passes through the constriction section 5311, reaching its fastest speed at the throat 5312. The speed gradually decreases as it passes through the expansion section 5313. The throats 5312 of the multiple Venturi tubes 531 gradually decrease along the direction of liquid flow. Furthermore, the lengths of the constriction section 5311 and the expansion section 5313 of the multiple Venturi tubes 531 are consistent, which gradually increases the flow rate of the liquid. This results in a more uniform mixing of the raw materials and water when transporting the raw materials in the sample tank 21.

[0032] Please see Figure 2 A monitoring branch pipe 55 is fixedly installed on the main pipe 53, and a monitoring pump 56 is fixedly installed on the monitoring branch pipe 55. The monitoring branch pipe 55 is located between the feed pump 54 and the water tank 4. The monitoring pump 56 and the feed pump 54 are of the same type and are controlled by the same controller. By controlling the flow rates of the monitoring pump 56 and the feed pump 54, the flow rate ratio between the main pipe 53 and the monitoring branch pipe 55 is controlled, so that the amount of liquid entering the tank 1 and the display cylinder 32 at the same time is proportional. Thus, the liquid level in the tank 1 can be displayed by the liquid level in the display cylinder 32. A monitoring inlet pipe 58 is provided between the feed branch pipe 57 and the display cylinder 32, and a three-way valve is provided between the monitoring inlet pipe 58 and the monitoring branch pipe 55, so that the feeding of the monitoring inlet pipe 58 can be controlled by controlling the three-way valve.

[0033] In this embodiment, a rinsing pipe 51 is fixedly installed on the water tank 4, and a rinsing pump 52 is fixedly installed on the rinsing pipe 51. The other end of the rinsing pipe 51 is positioned above the sample loading tank 21. When the rinsing pump 52 operates, it can drive the water in the water tank 4 to flush out along the rinsing pipe 51, thereby rinsing the inside of the sample loading tank 21. This facilitates flushing all the culture medium raw materials into the main pipe 53, avoiding ratio errors and the presence of residual culture medium raw materials in the sample loading tank 21 that could lead to contamination by bacteria.

[0034] It should be noted that the other end of the main pipe 53 and the monitoring branch pipe 55 can be connected to a recovery tank to keep the liquid inside the main pipe 53 and the monitoring branch pipe 55 flowing and to prevent the liquid from standing still and breeding bacteria.

[0035] A feeding method for an automatic seed tank feeding device, the feeding method being as follows: S1. Feeding: First, check if the sample loading tank 21 is clean and confirm that there is no residual material inside. Then, add the weighed solid or liquid culture medium raw material through the top opening of the sample loading tank 21. Because the sample loading tank 21 has a frustum-shaped structure and a sloping inner wall, the added raw material can slide naturally to the bottom outlet tube 23 area under gravity, facilitating subsequent transportation. After feeding, the motor on the crossbeam 24 can be started, causing the rotating shaft 25 to drive the rotating blade 26 to rotate slowly, initially loosening and distributing the raw material in the tank to prevent clumping or sticking to the wall.

[0036] S2. Adjustment: Based on the final liquid level required for the fermentation process, the operator rotates the adjusting vane 37 on the monitoring mechanism 3. The adjusting vane 37 drives the ball screw 38 via a thread, which in turn moves the piston 35 up and down within the display cylinder 32. The lower surface position of the piston 35 corresponds to the preset liquid level stop point. During adjustment, the limit rod 39 slides along the protective frame 31 to ensure that the piston 35 only moves vertically. After the position is set, the contact plate 36 is fixed relative to the piston 35, awaiting a subsequent trigger signal.

[0037] S3. Feeding: Start the feed pump 54. Water from tank 4 is transported through main pipe 53. Simultaneously, start the motor of the sampling mechanism 2. The motor output shaft drives the rotating shaft 25 and the auger 27 at the bottom to rotate. The auger 27 is located inside the sample outlet pipe 23. Its spiral blades continuously and evenly push the raw material accumulated at the inlet of the sample outlet pipe 23 into the main pipe 53. In the venturi section 531, the water flow is accelerated through the contraction section 5311, forming a negative pressure at the throat 5312, further drawing in and mixing the raw material. Subsequently, the flow rate returns to a stable state in the expansion section 5313, achieving preliminary homogenization and transportation of the raw material and water. The mixture is continuously injected into tank 1 through the feed branch pipe 57.

[0038] S4. Simultaneously with the start of the feed pump 54, the monitoring pump 56 operates synchronously. The monitoring pump 56 and feed pump 54 are driven by the same controller, and their flow rates are set in a fixed ratio to ensure a constant flow ratio between the main pipe 53 and the monitoring branch pipe 55. The liquid in the monitoring branch pipe 55 enters the bottom of the display cylinder 32 through the monitoring inlet pipe 58, pushing the piston 33 upwards slowly. Since the liquid level in the display cylinder 32 is proportional to the liquid inlet volume of the tank 1, the rate of liquid level rise in the display cylinder 32 corresponds to the actual rate of liquid level rise in the tank, thus allowing for a direct and synchronous simulation of the liquid level changes inside the tank from outside.

[0039] S5. Rinsing: When the raw material in the sample loading tank 21 is about to be emptied, start the rinsing pump 52. The cleaning water in the water tank 4 is sprayed into the upper part of the sample loading tank 21 through the rinsing pipe 51 at a certain flow rate. It is flushed along the inner wall to thoroughly flush the residual raw material attached to the tank wall and the rotating blade 26 into the sample outlet pipe 23, ensuring that all the raw material enters the main pipe 53 and avoiding ratio errors or cross-contamination due to residue.

[0040] S6. Volume Adjustment: As the liquid level in the display cylinder 32 continues to rise, piston 1 33 gradually approaches piston 2 35. When the liquid level reaches the preset height, contact piece 1 34 on piston 1 33 contacts contact piece 2 36 on piston 2 35, connecting the circuit and generating an electrical signal. This signal is transmitted to the PLC controller, which then issues a command to simultaneously close the three-way valves on the feed branch pipe 57 and the monitoring sample inlet pipe 58, thereby achieving automatic volume adjustment and stopping of feeding in tank 1.

[0041] S7. After feeding is completed, the feed pump 54 and the monitoring pump 56 can switch to low-speed circulation mode to keep the water in the main pipe 53 and the monitoring branch pipe 55 in a flowing state, so as to avoid the growth of microorganisms due to stagnant water. The system can continue to run until the next batch of feeding begins, ensuring that the pipeline is clean and the system is available immediately.

[0042] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus.

[0043] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. An automatic seed tank feeding device, comprising a tank body (1); characterized in that, The sample addition mechanism (2) is used for the automatic addition of culture medium raw materials; The monitoring unit (3) is used to monitor the liquid level in the tank (1); The sample injection mechanism (5) is connected to the water tank (4) and is used for communication between the water tank (4), the sample addition mechanism (2), the tank body (1), and the monitoring mechanism (3).

2. The automatic seed tank feeding device according to claim 1, characterized in that: The sample feeding mechanism (5) includes a main pipe (53), one end of which is fixedly connected to the water tank (4), a feed pump (54) is fixedly installed on the main pipe (53), and a feed branch pipe (57) is provided between the main pipe (53) and the tank body (1).

3. The automatic seed tank feeding device according to claim 2, characterized in that: A monitoring branch pipe (55) is fixedly installed on the main pipe (53), and a monitoring pump (56) is fixedly installed on the monitoring branch pipe (55). The monitoring branch pipe (55) is located between the feed pump (54) and the water tank (4).

4. The automatic seed tank feeding device according to claim 2, characterized in that: The monitoring mechanism (3) includes a protective frame (31), which is fixedly installed on the tank body (1). A display tube (32) is fixedly installed inside the protective frame (31). A piston one (33) is slidably installed inside the display tube (32) near the bottom. A piston two (35) is slidably installed inside the display tube (32) near the top. A monitoring injection tube (58) is provided between the feed branch pipe (57) and the display tube (32).

5. The automatic seed tank feeding device according to claim 4, characterized in that: A contact piece 1 (34) is fixedly installed on the piston 1 (33), and a contact piece 2 (36) is fixedly installed on the piston 2 (35). The contact piece 1 (34) and the contact piece 2 (36) are electrically connected.

6. The automatic seed tank feeding device according to claim 5, characterized in that: A ball screw (38) is fixedly installed at the top of the piston (35). The ball screw (38) is slidably connected to the protective frame (31) and the display cylinder (32). An adjusting plate (37) is threadedly connected to the surface of the ball screw (38). A limit rod (39) is fixedly installed at the top of the ball screw (38) through a bracket. The limit rod (39) is slidably connected to the protective frame (31). The adjusting plate (37) is rotatably connected to the protective frame (31).

7. The automatic seed tank feeding device according to claim 1, characterized in that: The sample addition mechanism (2) includes a sample addition tank (21), a sample outlet tube (23) is fixedly installed at the bottom of the sample addition tank (21), the sample outlet tube (23) is fixedly connected to the main pipe (53), an auger (27) is provided inside the sample outlet tube (23), and multiple venturi tubes (531) are provided on the main pipe (53) near the sample outlet tube (23).

8. The automatic seed tank feeding device according to claim 7, characterized in that: A crossbeam (24) is fixedly installed at the top of the sample addition tank (21). A motor is fixedly installed on the crossbeam (24). A rotating shaft (25) is fixedly connected to the output shaft of the motor. A rotating blade (26) is fixedly installed on the surface of the rotating shaft (25). The rotating shaft (25) is fixedly connected to the auger (27).

9. An automatic seed tank feeding device according to claim 7, characterized in that: A rinsing pipe (51) is fixedly installed on the water tank (4), and a rinsing pump (52) is fixedly installed on the rinsing pipe (51). The other end of the rinsing pipe (51) is located above the sample addition tank (21).

10. An automatic seed tank feeding method, used in the automatic seed tank feeding device according to any one of claims 1-9, characterized in that, Includes the following steps: S1. Adding raw materials: Add the raw materials into the sample addition container (21); S2. Adjustment: According to the required liquid level height of the tank (1), rotate the adjusting vane (37) to drive the ball screw (38) and piston (35) to move down to the preset position in the display cylinder (32); S3. Feeding: Start the feed pump (54) and motor. The motor drives the rotating shaft (25) to rotate the auger (27) and push the raw material in the sample tank (21) evenly and stably to the main pipe (53), and then flow into the tank body (1) through the feed branch pipe (57). S4. Divert the flow and start the feed pump (54) and the monitoring pump (56) at the same time, so that the flow ratio of the main pipe (53) and the monitoring branch pipe (55) is constant, and the liquid in the monitoring branch pipe (55) is transported to the display cylinder (32) through the monitoring injection tube (58); S5. Rinse, start the rinsing pump (52) and rinse the sample tank (21) through the rinsing pipe (51) so that all the raw materials enter the tank (1); S6. Volume adjustment: As the liquid continues to enter the display cylinder (32), piston one (33) gradually moves upward until contact piece one (34) contacts contact piece two (36) and generates an electrical signal. After receiving the signal, the PLC controller synchronously controls the valves on the feed branch pipe (57) and the monitoring sample inlet pipe (58) to stop feeding and realize the stop feeding. S7, Circulation: The feed pump (54) and monitoring pump (56) continue to work to keep the liquid flowing in the pipeline and prevent the growth of miscellaneous bacteria.

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