Targeted compound probiotic preparation for producing SOD eggs, feed and production method

Abstract

The invention discloses a targeted compound probiotic preparation for producing SOD (superoxide dismutase) eggs, which is prepared from the following active ingredients in percentage by mass: 5 to 15 percent of bacillus licheniformis, 3 to 10 percent of pseudomonas strain NJ-62, 15 to 30 percent of saccharomyces boulardii, 10 to 20 percent of lactobacillus plantarum, 8 to 15 percent of bacillus subtilis, 10 to 20 percent of candida utilis and 5 to 15 percent of lactobacillus acidophilus. The invention relates to the technical field of healthy breeding of livestock and poultry and production of functional food, in particular to integrated production of'safety 'and'function', specifically, targeted biological detoxification and nutrition metabolism induction technologies are deeply integrated for the first time, and complex probiotic groups remove antibiotics, heavy metals and other endogenous and exogenous pollutants in advance, so that a'clean 'physiological environment is created for laying hens; on the basis, efficient synthesis of SOD is specifically induced, and the problem of potential safety hazards of the functional eggs is fundamentally solved.

Description

Technical Field

[0001] This invention relates to the field of livestock and poultry health breeding and functional food production technology, specifically to a targeted compound probiotic preparation, feed and production method for producing SOD eggs. Background Technology

[0002] As consumers increasingly value the nutritional and health benefits of food, the market demand for functional poultry egg products, such as selenium-enriched eggs, omega-3 eggs, and SOD eggs, continues to expand. Among them, SOD eggs, rich in superoxide dismutase, have attracted much attention due to their potential health benefits, including antioxidant properties, anti-aging effects, and enhanced immunity.

[0003] Currently, the main technical approach to producing SOD-enriched eggs involves adding substances such as selenium, vitamin E, and polysaccharides to the feed of laying hens. This induces the hens to synthesize more SOD through nutrient metabolism and enrich it in the eggs. However, existing technologies have significant shortcomings: First, the induction efficiency is unstable, and the SOD activity in eggs fluctuates greatly, making it difficult to reach and stabilize at a high functional standard (e.g., ≥200 U / g). Second, the common problems of heavy metal pollution and antibiotic residues in feed sources in intensive farming directly affect the health of laying hens and the safety of eggs, and traditional SOD enrichment technologies lack solutions to these issues, potentially leading to safety risks in the produced "functional eggs." Finally, if the exogenously added probiotics cannot effectively couple with the host's intestinal flora, the colonization rate will be low, and the functional performance will be unstable.

[0004] Therefore, there is an urgent need to develop an integrated production technology that can simultaneously achieve "efficient induction of SOD enrichment" and "proactive protection of egg safety" in order to stably produce high-quality, truly safe functional SOD eggs. Summary of the Invention

[0005] To address the shortcomings of existing technologies, this invention provides a targeted compound probiotic preparation, feed, and production method for producing SOD-enriched eggs, solving the problems of unstable SOD enrichment efficiency and failure to consider the safety risks at the source of eggs in existing SOD egg production technologies.

[0006] To achieve the above objectives, the present invention provides the following technical solution: a targeted compound probiotic preparation for producing SOD-contaminated eggs, comprising the following active ingredients by mass percentage: Bacillus licheniformis 5%-15%, Pseudomonas NJ-62 strain 3%-10%, Saccharomyces boulardii 15%-30%, Lactobacillus plantarum 10%-20%, Bacillus subtilis 8%-15%, Candida utilis 10%-20%, Lactobacillus acidophilus 5%-15%.

[0007] Preferably, the viable bacteria count of each active ingredient is not less than 1×10⁻⁶. 9CFU / g, and the total viable count of the preparation is not less than 5×10¹ 0 CFU / g.

[0008] Preferably, the formulation also includes a carrier and synergistic excipients; based on the total mass of the formulation, the synergistic excipients include: 10%-20% modified montmorillonite, 3%-8% chitosan oligosaccharide, and 2%-5% organic selenium yeast; the remainder is the carrier.

[0009] Preferably, a compound feed for producing SOD eggs comprises a basal diet and the compound probiotic preparation, wherein the amount of the compound probiotic preparation added is 0.2%-0.6% of the total mass of the compound feed.

[0010] Preferably, the basal diet also contains an SOD inducer; based on the total mass of the compound feed, the SOD inducer includes: vitamin E 150-250 IU / kg, and / or curcumin extract 0.01%-0.03%.

[0011] A method for producing SOD eggs includes: feeding laying hens with the compound feed for at least 14 days, and then collecting the eggs produced.

[0012] Preferably, the feeding is continuous feeding throughout the entire period, or a feeding pattern that begins induction two weeks before egg laying and continues until the egg laying period.

[0013] Preferably, the feeding method is mixed feeding, wherein the compound probiotic preparation is added to the diet at a rate of 0.3%-0.5%.

[0014] Preferably, the feeding method is water feeding, which includes: mixing the compound probiotic preparation, brown sugar and water at a mass ratio of 1:(1-3):(80-120), letting it stand at 25-37℃ for 18-36 hours to obtain fermentation liquid; diluting the fermentation liquid with water at a volume ratio of 1:(300-700) and feeding it to laying hens for drinking 3-7 days a week.

[0015] A method for preparing SOD-treated eggs includes: feeding the eggs to laying hens as described above and collecting the eggs they produce. Beneficial effects

[0016] This invention provides a targeted compound probiotic preparation, feed, and production method for producing SOD-treated eggs, which has the following beneficial effects: 1. Integrated production of "safety" and "function": It is the first to deeply integrate targeted biological detoxification and nutritional metabolism induction technology. The compound probiotic group first removes internal and external pollutants such as antibiotics and heavy metals, creating a "clean" physiological environment for laying hens. On this basis, it can specifically induce the efficient synthesis of SOD, fundamentally solving the safety hazards of functional eggs. 2. High and stable SOD enrichment efficiency: Driven by the dual engines of "exogenous organic selenium + endogenous probiotic metabolic regulation", the SOD synthesis capacity of organs such as the liver of laying hens is significantly enhanced, and the SOD activity of egg yolks is stably reached and exceeded the functional threshold of 200U / g, with small batch-to-batch differences. 3. Strong stability of gut microbiota: The formulation contains the original host microbiota isolated from the intestines of healthy laying hens, which greatly improves the success rate of intestinal colonization and ecological adaptability of exogenous functional probiotics, ensuring long-lasting and stable effects; 4. Flexible application methods and significant economic benefits: It provides two application schemes, namely feed additive and drinking water supplement, which can be adapted to farms of different sizes and conditions. The SOD produced eggs have high added value. At the same time, the increase in egg production rate and the decrease in mortality rate due to the improved health of the flock further improve the overall breeding benefits. Detailed Implementation

[0017] The technical solutions of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present invention.

[0018] This invention provides a technical solution: a targeted compound probiotic preparation for producing SOD-contaminated eggs, comprising the following active ingredients by weight percentage: Bacillus licheniformis 5%-15%, Pseudomonas NJ-62 strain 3%-10%, Saccharomyces boulardii 15%-30%, Lactobacillus plantarum 10%-20%, Bacillus subtilis 8%-15%, Candida utilis 10%-20%, Lactobacillus acidophilus 5%-15%.

[0019] In this embodiment, the number of viable bacteria in each active ingredient is not less than 1×10⁻⁶. 9 CFU / g, and the total viable count of the preparation is not less than 5×10¹ 0 CFU / g.

[0020] This embodiment is further configured to include a carrier and synergistic excipients; based on the total mass of the formulation, the synergistic excipients include: 10%-20% modified montmorillonite, 3%-8% chitosan oligosaccharide, and 2%-5% organic selenium yeast; the remainder is the carrier.

[0021] A compound feed for producing SOD eggs comprises a basal diet and the compound probiotic preparation, wherein the compound probiotic preparation is added at an amount of 0.2%-0.6% of the total mass of the compound feed.

[0022] In this embodiment, the basal diet is further configured to contain an SOD inducer; based on the total mass of the compound feed, the SOD inducer includes: vitamin E 150-250 IU / kg, and / or curcumin extract 0.01%-0.03%.

[0023] A method for producing SOD eggs includes: feeding laying hens with the compound feed for at least 14 days, and then collecting the eggs produced.

[0024] In this embodiment, the feeding is set to be continuous feeding throughout the entire period, or a feeding mode that begins induction two weeks before egg laying and continues until the egg laying period.

[0025] In this embodiment, the feeding method is mixed feeding, wherein the amount of compound probiotic preparation added to the diet is 0.3%-0.5%.

[0026] In this embodiment, the feeding method is water feeding, which includes: mixing the compound probiotic preparation, brown sugar and water at a mass ratio of 1:(1-3):(80-120), letting it stand at 25-37℃ for 18-36 hours to obtain fermentation liquid; diluting the fermentation liquid with water at a volume ratio of 1:(300-700) and feeding it to laying hens for drinking 3-7 days a week.

[0027] A method for preparing SOD-treated eggs includes: feeding the eggs to laying hens as described above and collecting the eggs they produce.

[0028] Example 1: Preparation of a targeted compound probiotic formulation 1. Activation and fermentation of microorganisms: Bacillus licheniformis, Pseudomonas NJ-62 strain, Saccharomyces boulardii, Lactobacillus plantarum, Bacillus subtilis, Candida utilis, Lactobacillus acidophilus, and host progeny cultured from the cecal contents of healthy Hy-Line Brown chickens were inoculated separately and subjected to high-density liquid fermentation under their respective suitable culture media and conditions.

[0029] 2. Cell collection: After fermentation, wet cells of each strain are collected separately using a centrifuge.

[0030] 3. Mixing and blending: The collected wet bacterial cells are mixed according to a preset ratio (ensuring that the number of viable bacteria after freeze-drying meets the standard described in claim 1). Then, 15% modified montmorillonite, 5% chitosan oligosaccharide, and 3% organic selenium yeast powder are added to the total mixture and stirred thoroughly in a mixer until homogeneous.

[0031] 4. Freeze-drying and packaging: The uniformly mixed materials are fed into a vacuum freeze dryer for low-temperature freeze-drying. After freeze-drying, the mixture is pulverized, passed through an 80-mesh sieve, and made into freeze-dried powder. The powder is then packaged in aluminum foil bags, sealed with nitrogen, and stored in a cool, dry place.

[0032] Example 2: Aquaculture trial of SOD-enhanced eggs produced using feed additives 1. Experimental design: 900 Lohmann pink laying hens aged 360 days and with similar health conditions were randomly divided into 3 groups, with 3 replicates in each group and 100 hens in each replicate.

[0033] Control group: fed with a basal diet.

[0034] Normal SOD group: fed with basic diet + 0.05% organic selenium yeast (i.e. traditional SOD egg production method).

[0035] The experimental group of this invention was fed a basic diet plus 0.4% of the targeted compound probiotic preparation prepared in Example 1 (which already contains an equal amount of organic selenium yeast).

[0036] Testing items control group Ordinary SOD group This invention test group Egg yolk SOD activity (U / g) 85 ± 12 165 ± 25 245 ± 18 Cadmium residue in eggs (μg / kg) 8.5 ± 1.2 7.8 ± 1.0 3.1 ± 0.5 (↓63%) Lead residue in eggs (μg / kg) 15.2 ± 2.1 14.5 ± 1.8 5.8 ± 0.9 (↓62%) Detection rate of florfenicol metabolites 10% 10% 0% Egg production rate during the trial period 88.5% 89.2% 93.8 2. Test period: 7 days for the pre-test period and 28 days for the formal test period.

[0037] 3. Test Results: On day 28 of the testing period, 60 eggs were randomly collected from each group for testing. The average results are shown in the table below: The results show that the method of the present invention significantly improves the SOD activity of eggs (far exceeding the 200 U / g standard), while also showing significant effects in removing heavy metals and antibiotic residues and positively promoting egg production performance.

[0038] Example 3: Application of SOD-producing eggs using the drinking water supplementation method In a large-scale egg-laying farm, 5,000 laying hens in one chicken house were selected, and the drinking water solution of this invention was applied.

[0039] 1. Application method: Every Monday, mix 5 kg of the targeted compound probiotic preparation prepared in Example 1, 10 kg of brown sugar, and 500 L of clean drinking water in a fermentation tank and ferment at 32℃ for 24 hours. Add the fermentation liquid to the daily drinking water system at a ratio of 1:500 and provide it to the chickens for free drinking for 3 consecutive days (Monday to Wednesday).

[0040] 2. Application Results: After four weeks of continuous application, random sampling of eggs showed that the SOD activity in the yolks remained stable between 220-260 U / g, the heavy metal residue levels were better than the national standards for pollution-free eggs, the condition of the chicken feces was significantly improved, and intestinal health indicators were enhanced. This batch of eggs was sold under the brand "Probiotic-Enriched SOD Nutritional Eggs," resulting in a significant market premium.

[0041] It should be noted that, in this document, relational terms such as "first" and "second" are used merely to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such a process, method, article, or apparatus. Without further limitations, the phrase "comprising an element defined as..." does not exclude the presence of other identical elements in the process, method, article, or apparatus that includes said element.

[0042] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A targeted compound probiotic preparation for producing SOD-treated eggs, characterized in that, Composed of the following active ingredients by weight percentage: Bacillus licheniformis 5%-15%, Pseudomonas NJ-62 strain 3%-10%, Saccharomyces boulardii 15%-30%, Lactobacillus plantarum 10%-20%, Bacillus subtilis 8%-15%, Candida utilis 10%-20%, Lactobacillus acidophilus 5%-15%.

2. The targeted compound probiotic preparation according to claim 1, characterized in that, The viable bacteria count of each active ingredient is not less than 1×10⁻⁶. 9 CFU / g, and the total viable count of the preparation is not less than 5×10¹ 0 CFU / g.

3. The targeted compound probiotic preparation according to claim 1 or 2, characterized in that, It also includes a carrier and synergistic excipients; based on the total mass of the formulation, the synergistic excipients include: 10%-20% modified montmorillonite, 3%-8% chitosan oligosaccharide, and 2%-5% organic selenium yeast; the remainder is the carrier.

4. A compound feed for producing SOD-treated eggs, characterized in that, The diet comprises a basal diet and a compound probiotic preparation as described in any one of claims 1-3, wherein the amount of the compound probiotic preparation added is 0.2%-0.6% of the total mass of the compound feed.

5. The compound feed according to claim 4, characterized in that, The basal diet also contains an SOD inducer; based on the total mass of the compound feed, the SOD inducer includes: vitamin E 150-250 IU / kg, and / or curcumin extract 0.01%-0.03%.

6. A method for producing SOD-rich eggs, characterized in that, include: Egg-laying hens were fed the compound feed as described in claim 4 or 5 for at least 14 days, and the eggs were then collected.

7. The method according to claim 6, characterized in that, The feeding refers to feeding throughout the entire period, or adopting a feeding pattern that begins induction two weeks before egg laying and continues until the egg laying period.

8. The method according to claim 6 or 7, characterized in that, The feeding method is to mix the compound probiotic preparation with feed, wherein the amount of compound probiotic preparation added to the diet is 0.3%-0.5%.

9. The method according to claim 6 or 7, characterized in that, The feeding method is water feeding, which includes: mixing the compound probiotic preparation, brown sugar and water at a mass ratio of 1:(1-3):(80-120), letting it stand at 25-37℃ for 18-36 hours to obtain fermentation liquid; diluting the fermentation liquid with water at a volume ratio of 1:(300-700) and feeding it to laying hens for drinking 3-7 days a week.

10. A method for preparing SOD-treated eggs, characterized in that, include: The laying hens are fed using the method described in any one of claims 6-9, and their eggs are collected.