Vehicle-mounted refreshing and anti-fatigue traditional Chinese medicine composition, fragrance essential oil and preparation method of fragrance essential oil

Abstract

The embodiment of the invention provides a vehicle-mounted refreshing and anti-fatigue traditional Chinese medicine composition, fragrance essential oil and a preparation method of the fragrance essential oil. The vehicle-mounted refreshing and anti-fatigue traditional Chinese medicine composition comprises 1-12 parts of ginseng, 1-12 parts of rhizoma atractylodis, 1-3 parts of rhizoma acori graminei, 1-12 parts of purple perilla, 1-12 parts of agastache rugosus, 1-12 parts of mint, 1-12 parts of sandalwood, 1-12 parts of honeysuckle and 1-12 parts of liquorice. According to the vehicle-mounted refreshing anti-fatigue traditional Chinese medicine composition provided by the embodiment of the invention, volatile medicinal active ingredients can exist, and people can absorb the medicinal active ingredients in the traditional Chinese medicine composition through respiratory tracts or skin, so that the medicine can be absorbed in a non-invasive manner while feeling pleasant fragrance. The traditional Chinese medicine composition is used for regulating qi and blood, invigorating spleen to eliminate dampness and dredging channels and collaterals, so that the effects of relieving fatigue and refreshing are effectively realized.

Description

A car-mounted energizing and fatigue-relieving traditional Chinese medicine composition, aromatherapy essential oil, and its preparation method Technical Field

[0001] This application relates to the field of essential oil technology, and in particular to a car-mounted refreshing and fatigue-relieving traditional Chinese medicine composition, a car-mounted refreshing and fatigue-relieving aromatherapy essential oil, and a method for preparing a car-mounted refreshing and fatigue-relieving aromatherapy essential oil. Background Technology

[0002] In today's fast-paced society, people are prone to fatigue and mental exhaustion due to long-term high-intensity work, study, and life pressures. Symptoms include dizziness, poor concentration, memory loss, and low work efficiency. Traditionally, methods to relieve fatigue mainly include medication, beverage intake (such as coffee and tea), and rest. However, these methods have certain limitations. For example, medication may lead to drug dependence or side effects, stimulants such as caffeine can easily cause palpitations or sleep disorders, and their effects are limited in duration.

[0003] In existing technologies, some fragrance products primarily improve mood to some extent by providing scents that people generally enjoy, thereby alleviating fatigue to a certain degree. However, the effect of relieving fatigue at the emotional level is relatively limited. Summary of the Invention

[0004] This application provides a vehicle-mounted, energizing, and fatigue-relieving traditional Chinese medicine composition, aromatherapy essential oil, and its preparation method, to achieve the effect of relieving fatigue.

[0005] This application discloses a vehicle-mounted energizing and fatigue-relieving traditional Chinese medicine composition, which includes 1-12 parts of ginseng, 1-12 parts of Atractylodes lancea, 1-3 parts of Acorus tatarinowii, 1-12 parts of Perilla frutescens, 1-12 parts of Pogostemon cablin, 1-12 parts of Mentha haplocalyx, 1-12 parts of Santalum album, 1-12 parts of Lonicera japonica, and 1-12 parts of Glycyrrhiza uralensis.

[0006] Optionally, the in-vehicle energizing and fatigue-relieving traditional Chinese medicine composition is used to relieve fatigue and refresh the mind, including 3-12 parts of ginseng, 3-12 parts of Atractylodes lancea, 1-3 parts of Acorus tatarinowii, 3-12 parts of Perilla frutescens, 3-12 parts of Pogostemon cablin, 2-7 parts of peppermint, 3-12 parts of sandalwood, 3-12 parts of honeysuckle, and 2-7 parts of licorice.

[0007] This application also discloses a car-mounted refreshing and fatigue-relieving aromatherapy essential oil, which includes an essential oil made from the car-mounted refreshing and fatigue-relieving traditional Chinese medicine composition as described in this application embodiment.

[0008] This application also discloses a method for preparing a car-mounted refreshing and anti-fatigue aromatherapy essential oil as described in this application embodiment. The method includes: mixing 1-12 parts of ginseng, 1-12 parts of Atractylodes lancea, 1-3 parts of Acorus tatarinowii, 1-12 parts of Perilla frutescens, 1-12 parts of Pogostemon cablin, 1-12 parts of peppermint, 1-12 parts of sandalwood, 1-12 parts of honeysuckle, and 1-12 parts of licorice to obtain a car-mounted refreshing and anti-fatigue traditional Chinese medicine composition; loading the car-mounted refreshing and anti-fatigue traditional Chinese medicine composition into a supercritical fluid extraction device and performing supercritical fluid extraction on the car-mounted refreshing and anti-fatigue traditional Chinese medicine composition to obtain a primary extracted essential oil; loading the primary extracted essential oil into a molecular distillation purification device and performing molecular distillation purification on the primary extracted essential oil to obtain the car-mounted refreshing and anti-fatigue aromatherapy essential oil.

[0009] Optionally, the step of loading the in-vehicle energizing and fatigue-relieving traditional Chinese medicine composition into a supercritical fluid extraction device and performing supercritical fluid extraction on the in-vehicle energizing and fatigue-relieving traditional Chinese medicine composition to obtain a preliminary extracted essential oil includes: grinding the in-vehicle energizing and fatigue-relieving traditional Chinese medicine composition to obtain a traditional Chinese medicine composition powder of 40-70 mesh; loading the traditional Chinese medicine composition powder into a supercritical fluid extraction device and performing supercritical fluid extraction on the traditional Chinese medicine composition powder to obtain a preliminary extracted essential oil.

[0010] Optionally, supercritical fluid extraction satisfies at least one of the following: the extractant for supercritical fluid extraction is carbon dioxide; the temperature range for supercritical fluid extraction is 40℃~50℃; the pressure range for supercritical fluid extraction is 15MPa~35MPa; the extraction time for supercritical fluid extraction is 1h~3h; and the flow rate for supercritical fluid extraction is 300L / h~900L / h.

[0011] Optionally, the molecular distillation purification apparatus includes a preheater, an evaporator, an external primary condenser, and an external secondary condenser; an internal condenser is provided inside the evaporator; the step of loading the primary extracted essential oil into the molecular distillation purification apparatus and performing molecular distillation purification on the primary extracted essential oil to obtain a car-mounted refreshing and anti-fatigue fragrance essential oil includes: passing the primary extracted essential oil into the preheater for preheating treatment; passing the preheated primary extracted essential oil into the evaporator for evaporation treatment, and separating and purifying the essential oil components in the primary extracted essential oil through the internal condenser to obtain the car-mounted refreshing and anti-fatigue fragrance essential oil; passing the car-mounted refreshing and anti-fatigue fragrance essential oil sequentially through the external primary condenser and the external secondary condenser to obtain the cooled car-mounted refreshing and anti-fatigue fragrance essential oil.

[0012] Optionally, the molecular distillation purification satisfies at least one of the following: the preheating temperature of the preheater is 60℃~80℃; the evaporation temperature of the evaporator is 155℃~180℃; the internal condensation temperature of the internal condenser is 20℃~25℃; the external primary condensation temperature of the external primary condenser is -22℃~-30℃; and the external secondary condensation temperature of the external secondary condenser is -40℃~-78℃.

[0013] Optionally, the primary extraction rate of essential oils from supercritical fluid extraction is 2% to 4%, and / or the distillation rate of car-use refreshing and anti-fatigue fragrance essential oils purified by molecular distillation is 48% to 57%.

[0014] An application of a car-mounted refreshing and anti-fatigue aromatherapy essential oil, the car-mounted refreshing and anti-fatigue aromatherapy essential oil described in the embodiments of this application, or the car-mounted refreshing and anti-fatigue aromatherapy essential oil prepared by the method described in the embodiments of this application, is used to relieve fatigue and refresh the mind.

[0015] The embodiments of this application include the following advantages: This application provides a vehicle-mounted, invigorating, and fatigue-relieving traditional Chinese medicine composition, which may contain volatile medicinal active ingredients. People can absorb the medicinal active ingredients in the composition through the respiratory tract or skin, experiencing a pleasant aroma while non-invasively absorbing the drug. The traditional Chinese medicine composition regulates qi and blood, strengthens the spleen and eliminates dampness, and unblocks meridians, effectively relieving fatigue and refreshing the mind. Attached Figure Description

[0016] Figure 1 is a comparative schematic diagram of behavioral parameters of mice in an open field experiment provided in this application; Figure 2 is a comparative schematic diagram of movement trajectories of mice in an open field experiment provided in this application; Figure 3 is a total ion chromatogram of the car-mounted refreshing and anti-fatigue aromatherapy essential oil provided in this application; Figure 4 is a gas chromatogram of the car-mounted refreshing and anti-fatigue aromatherapy essential oil provided in this application; Figure 5 is a comparative schematic diagram of behavioral parameters of mice in another open field experiment provided in this application; Figure 6 is a comparative schematic diagram of movement trajectories of mice in another open field experiment provided in this application; Figure 7 is a comparative schematic diagram of an elevated cross-shaped structure provided in this application. Figure 8 is a comparative schematic diagram of the behavioral parameters of mice in the maze test; Figure 9 is a comparative schematic diagram of the movement trajectory of mice in the elevated cross maze test provided in this application; Figure 10 is a comparative schematic diagram of the struggling behavior of mice in the tail-suspended test provided in this application; Figure 11 is a comparative schematic diagram of the biochemical data of mice in the biochemical test provided in this application; Figure 12 is a comparative schematic diagram of the neurotransmitter content in the brain tissue of mice in the biochemical test provided in this application; Figure 13 is a comparative schematic diagram of the mouse brain tissue slices provided in this application. Detailed Implementation

[0017] To make the above-mentioned objectives, features and advantages of this application more apparent and understandable, the application will be further described in detail below with reference to the accompanying drawings and specific embodiments.

[0018] This application discloses a vehicle-mounted energizing and fatigue-relieving traditional Chinese medicine composition, which includes 1-12 parts of ginseng, 1-12 parts of Atractylodes lancea, 1-3 parts of Acorus tatarinowii, 1-12 parts of Perilla frutescens, 1-12 parts of Pogostemon cablin, 1-12 parts of Mentha haplocalyx, 1-12 parts of Santalum album, 1-12 parts of Lonicera japonica, and 1-12 parts of Glycyrrhiza uralensis.

[0019] Specifically, Traditional Chinese Medicine (TCM) generally believes that fatigue and lethargy are related to pathogenesis such as spleen and stomach weakness, insufficient qi and blood, and phlegm-dampness obstruction. The in-vehicle energizing and fatigue-relieving TCM composition provided in this application includes Atractylodes lancea and ginseng, which have spleen-strengthening and dampness-drying effects. By enhancing the spleen and stomach's digestive function through Atractylodes lancea and ginseng, the production of dampness can be reduced at its source, thereby alleviating heaviness, fatigue, and weakness. Licorice has spleen-strengthening and qi-tonifying effects, which can help strengthen the spleen and stomach, clear dampness, and reduce fatigue. Aromatic herbs such as patchouli, perilla, acorus tatarinowii, peppermint, and sandalwood can unblock meridians, relieve dizziness and lethargy caused by phlegm-dampness obstruction, and make one feel refreshed. Meanwhile, honeysuckle has heat-clearing and detoxifying effects, which can also clear the head and eyes. The in-vehicle energizing and fatigue-relieving traditional Chinese medicine composition provided in this application embodiment combines different drugs in appropriate proportions to regulate qi and blood, strengthen the spleen and eliminate dampness, and dredge the meridians. It can effectively improve symptoms of fatigue and lethargy, thereby relieving fatigue and refreshing the mind.

[0020] This in-vehicle invigorating and fatigue-relieving traditional Chinese medicine composition contains volatile medicinal active ingredients, allowing the human body to absorb it through the respiratory tract or skin. While enjoying the pleasant aroma of the composition, the body can also take the medicine non-invasively. This in-vehicle invigorating and fatigue-relieving traditional Chinese medicine composition treats and improves symptoms of fatigue and lethargy. In addition to improving mood through its aroma, it further improves physical condition through drug treatment, fundamentally achieving the effects of relieving fatigue and refreshing the mind.

[0021] In one embodiment of this application, the vehicle-mounted invigorating and fatigue-relieving traditional Chinese medicine composition is used to achieve the effects of relieving fatigue and refreshing the mind, including 3-12 parts of ginseng, 3-12 parts of Atractylodes lancea, 1-3 parts of Acorus tatarinowii, 3-12 parts of Perilla frutescens, 3-12 parts of Pogostemon cablin, 2-7 parts of peppermint, 3-12 parts of sandalwood, 3-12 parts of honeysuckle, and 2-7 parts of licorice.

[0022] Specifically, the in-vehicle energizing and fatigue-relieving traditional Chinese medicine composition, when formulated in this ratio, can better regulate qi and blood, strengthen the spleen and eliminate dampness, and dredge the meridians, thus better relieving fatigue and refreshing the mind.

[0023] This application also discloses a car-mounted refreshing and fatigue-relieving aromatherapy essential oil, including an essential oil made from the car-mounted refreshing and fatigue-relieving traditional Chinese medicine composition as described in this application.

[0024] In this embodiment, to further increase the concentration of the medicinal active ingredients and more conveniently provide users with the aroma of the traditional Chinese medicine composition, the medicinal active ingredients can be extracted from the composition and made into essential oils for users. The car-mounted invigorating and fatigue-relieving aromatherapy essential oil made from the traditional Chinese medicine composition can be conveniently used by users in various scenarios, such as the office, home, and vehicle. The aromatherapy essential oil extracted from the traditional Chinese medicine composition can have a higher concentration of medicinal active ingredients, thus better achieving the effects of relieving fatigue and refreshing the mind.

[0025] As a specific example of this application, an in-vehicle refreshing and anti-fatigue aromatherapy essential oil can be used in the driver's cabin of a vehicle. The in-vehicle refreshing and anti-fatigue aromatherapy essential oil is placed in an in-vehicle aromatherapy generator. During the driving process, the in-vehicle refreshing and anti-fatigue aromatherapy essential oil provides the driver in the cabin with medicinal active ingredients to relieve the driver's fatigue caused by driving.

[0026] Furthermore, the in-car fragrance generator can be linked with an in-vehicle fatigue monitoring system. With the user's informed consent, the in-vehicle fatigue monitoring system can use various devices such as cameras, microphones, steering wheel torque sensors, seat gravity sensors, and cockpit radar to monitor whether the driver is fatigued. If the system determines that the driver is fatigued, it can control the in-car fragrance generator to release essential oils to alleviate driver fatigue.

[0027] In addition, the car-mounted refreshing and fatigue-relieving aromatherapy essential oil can also be used in enclosed indoor spaces such as offices, homes, hotels, halls, and temples, as well as in non-enclosed outdoor spaces such as squares, parks, stadiums, and swimming pools. This application does not impose any restrictions on this.

[0028] In addition to being provided to users in the form of essential oil, car-mounted refreshing and fatigue-relieving aromatherapy essential oils can also be further processed into sachets, perfumes, or other forms for users to enjoy. This application does not impose any restrictions on this.

[0029] In specific implementations, the volatile components with medicinal properties extracted from the in-vehicle energizing and fatigue-relieving traditional Chinese medicine composition may include, for example, menthol, 6-methyl-3-(1-methylethyl)-7-oxabicyclo[4.1.0]heptane-2-one, elemol, dill oil, γ-eucalyptol, atractylodes lancea alcohol, β-eucalyptol, baicalein, α-santalol, 9 (10) -Z-α-trans-limonene alcohol, (E)-α-santalol, β-santalol, 2-methyl-5-[(1S,4R,6R)-6-methyl-5-methylene-6-bicyclo[2.2.1]heptyl]pent-2-en-1-ol, phytol, 7,9-di-tert-butyl-1-oxaspiro[4.5]dec-6,9-dien-2,8-dione, 2,3-dimethyl-5-(trifluoromethyl)-1,4-benzenediol, etc. These components can be absorbed by the human body through the respiratory tract or skin, and can relieve fatigue and refresh the mind. They can effectively improve symptoms such as dizziness, headache, and lethargy caused by spleen and stomach weakness and phlegm-dampness obstruction.

[0030] The in-car refreshing and fatigue-relieving aromatherapy essential oil provided in this application embodiment can be extracted using methods such as steam distillation, solvent extraction, pressing, adsorption, and subcritical extraction. This application does not impose any restrictions on this method.

[0031] Among these methods, steam distillation can use steam to carry out the medicinal active ingredients from the traditional Chinese medicine composition, followed by cooling and separation to obtain a car-refreshing and fatigue-relieving aromatherapy essential oil. Solvent extraction utilizes the principle of "like dissolves like," dissolving the medicinal active ingredients in an organic solvent while other components such as cellulose remain insoluble, thus achieving separation and extraction of the active ingredients to obtain the car-refreshing and fatigue-relieving aromatherapy essential oil. Pressing uses mechanical physical pressure to break the oil glands or cells in the traditional Chinese medicine composition to extract the essential oil. Adsorption uses an adsorbent to adsorb the medicinal active ingredients from the traditional Chinese medicine composition, thereby extracting the essential oil. Subcritical extraction uses a subcritical fluid between liquid and gaseous states as the extractant to extract the medicinal active ingredients from the traditional Chinese medicine composition, thus obtaining the car-refreshing and fatigue-relieving aromatherapy essential oil.

[0032] This application also discloses a method for preparing a car-mounted refreshing and anti-fatigue aromatherapy essential oil as described in this application embodiment. The method includes: step 101, mixing 1-12 parts of ginseng, 1-12 parts of Atractylodes lancea, 1-3 parts of Acorus tatarinowii, 1-12 parts of Perilla frutescens, 1-12 parts of Pogostemon cablin, 1-12 parts of peppermint, 1-12 parts of sandalwood, 1-12 parts of honeysuckle, and 1-12 parts of licorice to obtain a car-mounted refreshing and anti-fatigue traditional Chinese medicine composition; step 102, loading the car-mounted refreshing and anti-fatigue traditional Chinese medicine composition into a supercritical fluid extraction device and performing supercritical fluid extraction on the car-mounted refreshing and anti-fatigue traditional Chinese medicine composition to obtain a primary extracted essential oil; step 103, loading the primary extracted essential oil into a molecular distillation purification device and performing molecular distillation purification on the primary extracted essential oil to obtain a car-mounted refreshing and anti-fatigue aromatherapy essential oil.

[0033] Specifically, to increase the content of medicinal active ingredients in car-use refreshing and anti-fatigue aromatherapy essential oils, prevent the oxidation of these ingredients, and improve the overall purity of the essential oils, the raw materials of the car-use refreshing and anti-fatigue traditional Chinese medicine composition are mixed during the preparation process. This composition is then placed in a supercritical fluid extraction device for supercritical fluid extraction to obtain the initial extracted essential oil. Supercritical fluid extraction has high extraction efficiency and speed, and it is less likely to damage heat-sensitive components. Furthermore, supercritical fluid extraction can use carbon dioxide as the extractant, which protects the medicinal active ingredients and prevents oxidation. This results in higher overall purity of the final car-use refreshing and anti-fatigue aromatherapy essential oil, allowing it to better retain the medicinal effects of the traditional Chinese medicine composition.

[0034] Subsequently, the primary extracted essential oil can be further processed by molecular distillation purification equipment to improve the purity of the fragrance essential oil, resulting in a purer car-use refreshing and anti-fatigue fragrance essential oil with a more mellow aroma and clearer color.

[0035] Molecular distillation purification refers to a liquid-liquid separation technique that utilizes the evaporation of liquid molecules from the liquid surface upon heating, separating them based on differences in mean free path. Through molecular distillation purification, car-use refreshing and anti-fatigue aromatherapy essential oils can be purified within a relatively low temperature range, avoiding damage to the medicinal active ingredients. Simultaneously, it can more effectively separate the medicinal active ingredients from impurities, further improving the purity of the car-use refreshing and anti-fatigue aromatherapy essential oils.

[0036] In one embodiment of this application, the step of loading the in-vehicle energizing and fatigue-relieving traditional Chinese medicine composition into a supercritical fluid extraction device and performing supercritical fluid extraction on the in-vehicle energizing and fatigue-relieving traditional Chinese medicine composition to obtain a preliminary extracted essential oil includes: sub-step 11, grinding the in-vehicle energizing and fatigue-relieving traditional Chinese medicine composition to obtain a traditional Chinese medicine composition powder of 40-70 mesh; sub-step 12, loading the traditional Chinese medicine composition powder into a supercritical fluid extraction device and performing supercritical fluid extraction on the traditional Chinese medicine composition powder to obtain a preliminary extracted essential oil.

[0037] In practice, before supercritical fluid extraction of the traditional Chinese medicine composition, the composition is first ground into fine powder particles to improve the extraction efficiency and speed of subsequent supercritical fluid extraction. The powdered composition is then loaded into a supercritical fluid extraction device for supercritical extraction.

[0038] Specifically, the traditional Chinese medicine composition can be processed into a powder of 40-70 mesh. The powder of traditional Chinese medicine composition within this mesh range has a moderate fineness, and the active ingredients can be fully dissolved during the supercritical fluid extraction process without causing blockage to the supercritical fluid extraction process.

[0039] In one embodiment of this application, supercritical fluid extraction satisfies at least one of the following: the extractant for supercritical fluid extraction is carbon dioxide; the temperature range for supercritical fluid extraction is 40°C to 50°C; the pressure range for supercritical fluid extraction is 15MPa to 35MPa; the extraction time for supercritical fluid extraction is 1h to 3h; and the flow rate for supercritical fluid extraction is 300L / h to 900L / h.

[0040] Specifically, carbon dioxide can be used as the extractant in supercritical fluid extraction. Carbon dioxide is a recognized safe substance, preventing the extracted fragrance oil from containing solvent or other chemical residues. Through simple pressure reduction or temperature increase, carbon dioxide can be rapidly vaporized and separated from the extracted car-refreshing and anti-fatigue fragrance oil, eliminating the need for traditional high-temperature desolvation processes such as distillation, thus avoiding the destruction of medicinal active ingredients in the car-refreshing and anti-fatigue fragrance oil by high temperatures. Furthermore, carbon dioxide can be recycled, minimizing the generation of chemical waste during the extraction process and achieving clean production. Simultaneously, carbon dioxide can protect the medicinal active ingredients in the car-refreshing and anti-fatigue traditional Chinese medicine composition from oxidation during supercritical fluid extraction, thereby better increasing the content of medicinal active ingredients in the car-refreshing and anti-fatigue fragrance oil.

[0041] The temperature range for supercritical fluid extraction is 40℃~50℃, for example, 40℃, 42℃, 44℃, 46℃, 48℃, 50℃, etc. Within this temperature range, the medicinal active ingredients in the in-car energizing and fatigue-relieving traditional Chinese medicine composition are less likely to react and denature, thus allowing for greater retention of more medicinal active ingredients in the in-car energizing and fatigue-relieving aromatherapy essential oil.

[0042] The pressure range for supercritical fluid extraction is 15 MPa to 35 MPa, for example, 15 MPa, 20 MPa, 25 MPa, 30 MPa, 35 MPa, etc. Within this pressure range, the extractant can reach a supercritical state, with a density close to that of a liquid. At this pressure, the extractant can have good dissolving ability, allowing the active pharmaceutical ingredients to be fully dissolved from the in-vehicle energizing and fatigue-relieving traditional Chinese medicine composition.

[0043] The extraction time for supercritical fluid extraction is 1 to 3 hours, for example, 1 hour, 1.5 hours, 2 hours, 2.5 hours, and 3 hours. Within this extraction time range, the active pharmaceutical ingredients can be extracted relatively fully in a shorter extraction time, resulting in a high extraction efficiency in the preparation of fragrance essential oils.

[0044] The flow rate for supercritical fluid extraction is 300 L / h to 900 L / h, for example, 300 L / h, 400 L / h, 500 L / h, 6000 L / h, 700 L / h, 800 L / h, 900 L / h, etc. Within this flow rate range, a greater amount of extractant can come into full contact with the in-vehicle energizing and fatigue-relieving traditional Chinese medicine composition per unit time, thereby improving the extraction efficiency of the medicinal active ingredients in the in-vehicle energizing and fatigue-relieving traditional Chinese medicine composition.

[0045] In one embodiment of this application, the molecular distillation purification apparatus includes a preheater, an evaporator, an external primary condenser, and an external secondary condenser; an internal condenser is provided inside the evaporator; the step of loading the primary extracted essential oil into the molecular distillation purification apparatus and performing molecular distillation purification on the primary extracted essential oil to obtain a car-mounted refreshing and anti-fatigue fragrance essential oil includes: sub-step 21, passing the primary extracted essential oil into the preheater for preheating treatment; sub-step 22, passing the preheated primary extracted essential oil into the evaporator for evaporation treatment, and separating and purifying the essential oil components in the primary extracted essential oil through the internal condenser to obtain a car-mounted refreshing and anti-fatigue fragrance essential oil; sub-step 23, passing the car-mounted refreshing and anti-fatigue fragrance essential oil sequentially through the external primary condenser and the external secondary condenser to obtain a cooled car-mounted refreshing and anti-fatigue fragrance essential oil.

[0046] Specifically, the molecular distillation purification apparatus may include a preheater, an evaporator, an external primary condenser, and an external secondary condenser arranged in sequence. The evaporator contains an internal condenser.

[0047] Before molecular distillation purification, the primary extracted essential oil can be preheated in a preheater to reduce the time spent on subsequent evaporation. Then, molecular distillation purification can be performed. The preheated primary extracted essential oil is then passed into an evaporator for evaporation. The primary extracted essential oil is heated to a gaseous state in the evaporator. The medicinal active ingredients, being lighter and more volatile, preferentially reach the surface of the internal condenser in the evaporator and are condensed into a liquid state. This liquid is collected as the purified essential oil component, resulting in a car-use refreshing and anti-fatigue fragrance essential oil. Impurities in the primary extracted fragrance essential oil are less volatile than the medicinal active ingredients and are less likely to move to the internal condenser in the evaporator. Instead, they flow downwards along the evaporation surface of the evaporator, thus separating from the medicinal active ingredients. Subsequently, the car-mounted refreshing and anti-fatigue aromatherapy essential oil discharged from the internal condenser may still be at a relatively high temperature and may contain medicinal active ingredients that are not completely cooled and are still in a gaseous state. At this time, the aromatherapy essential oil can be passed through the external primary condenser and the external secondary condenser in sequence for further cooling treatment to obtain the car-mounted refreshing and anti-fatigue aromatherapy essential oil that has been cooled.

[0048] In one embodiment of this application, molecular distillation purification satisfies at least one of the following: the preheating temperature of the preheater is 60℃~80℃; the evaporation temperature of the evaporator is 155℃~180℃; the internal condensation temperature of the internal condenser is 20℃~25℃; the external primary condensation temperature of the external primary condenser is -22℃~-30℃; and the external secondary condensation temperature of the external secondary condenser is -40℃~-78℃.

[0049] Specifically, the preheating temperature of the preheater is 60℃~80℃, such as 60℃, 65℃, 70℃, 75℃, 80℃, etc. Within this temperature range, the temperature of the primary extracted essential oil can be increased in advance, which can effectively reduce the time spent on subsequent evaporation processes. At the same time, it can reduce the viscosity of the primary extracted essential oil to a certain extent, making it easier for the primary extracted essential oil to contact the evaporation surface of the evaporator more efficiently during the subsequent evaporation process, thereby improving evaporation efficiency.

[0050] The evaporator's evaporation temperature is 155℃~180℃, such as 155℃, 160℃, 165℃, 170℃, 175℃, 180℃, etc. Within this temperature range, the medicinal active ingredients of the primary extracted essential oil are not easily destroyed, and at the same time, the primary extracted essential oil can have better contact with the evaporation surface of the evaporator, evaporating into gaseous molecules.

[0051] The internal condenser has an internal condensation temperature of 20℃~25℃, such as 20℃, 21℃, 22℃, 23℃, 24℃, 25℃, etc. Within this temperature range, the gaseous medicinal active ingredients can come into contact with the surface of the internal condenser and be condensed into liquid form relatively efficiently. The liquid is then collected as purified essential oil components to obtain in-car refreshing and anti-fatigue fragrance essential oil.

[0052] The external primary condenser has an external condensing temperature range of -22℃ to -30℃, such as -22℃, -24℃, -26℃, -28℃, and -30℃. Within this temperature range, the fragrance essential oil discharged from the internal condenser can be cooled rapidly. Simultaneously, it effectively cools the in-car energizing and fatigue-relieving fragrance essential oil, which may contain partially cooled, gaseous medicinal active ingredients, into a liquid state.

[0053] The external secondary condenser has an external condensation temperature range of -40℃ to -78℃, such as -40℃, -50℃, -60℃, -70℃, and -78℃. Within this temperature range, the in-car energizing and fatigue-relieving fragrance essential oil discharged from the internal condenser can be further and more effectively cooled.

[0054] In one embodiment of this application, the initial extraction rate of essential oils from supercritical fluid extraction is 2% to 4%, and / or the distillation rate of car-use refreshing and anti-fatigue fragrance essential oils purified by molecular distillation is 48% to 57%.

[0055] Specifically, in this application embodiment, the primary essential oil is extracted using supercritical fluid extraction, with an extraction rate of 2% to 4%, which is higher than the pharmacopoeia limit standard for volatile oils contained in each herb in the herbal composition, thus achieving higher extraction efficiency.

[0056] Meanwhile, the embodiments of this application further purify the fragrance essential oil through molecular distillation, with a distillation rate of 48% to 57%, which fully preserves the medicinal active ingredients in the traditional Chinese medicine composition and effectively improves the purity of the car-mounted refreshing and anti-fatigue fragrance essential oil, making the refined fragrance essential oil have a purer aroma and a clearer color.

[0057] This application also provides an application of a car-mounted refreshing and anti-fatigue aromatherapy essential oil. The car-mounted refreshing and anti-fatigue aromatherapy essential oil described in this application, or the car-mounted refreshing and anti-fatigue aromatherapy essential oil prepared by the method described in this application, is used to relieve fatigue and refresh the mind.

[0058] The in-car invigorating and fatigue-relieving aromatherapy essential oil of this application embodiment contains medicinal active ingredients extracted from traditional Chinese medicine extracts. People can absorb these medicinal active ingredients through the respiratory tract or skin, experiencing a pleasant aroma while non-invasively absorbing the medication. Thus, the medicinal active ingredients in the traditional Chinese medicine composition regulate qi and blood, strengthen the spleen and eliminate dampness, and unblock meridians, effectively relieving fatigue and refreshing the mind.

[0059] To enable those skilled in the art to better understand this application, the following specific embodiments illustrate a traditional Chinese medicine composition, a fragrance essential oil, and a method for preparing the same. All materials and instruments used in the following embodiments are commercially available. Unless otherwise specified, all raw materials used in the following embodiments are of medical grade and can be purchased commercially. Furthermore, the term "water" in this application includes any feasible type of water suitable for medical applications, such as deionized water, distilled water, ion-exchange water, double-distilled water, high-purity water, and purified water.

[0060] Example 1 (1) Mix 3 parts ginseng, 3 parts Atractylodes lancea, 2 parts Acorus tatarinowii, 3 parts Perilla frutescens, 3 parts Pogostemon cablin, 7 parts Mentha haplocalyx, 12 parts Sandalwood, 12 parts Lonicera japonica, and 2 parts Glycyrrhiza uralensis to obtain a vehicle-mounted invigorating and fatigue-relieving traditional Chinese medicine composition; (2) Grind the traditional Chinese medicine composition using a grinder to obtain a 60-mesh powder; (3) Load the powder into a supercritical fluid extraction device and perform supercritical fluid extraction with CO2. The extraction parameters are: temperature 50 degrees Celsius, pressure 35 MPa. Pa, extraction time 3h, flow rate 900L / h, to obtain primary extracted essential oil, extraction rate 2.63%; (4) The primary extracted essential oil was loaded into a molecular distillation purification device and the primary extracted essential oil was purified by molecular distillation to obtain car-mounted refreshing and anti-fatigue fragrance essential oil, yield 48.6%; the molecular distillation purification parameters were: preheating temperature 80℃; evaporation temperature: 155℃; internal cooling 20℃; external first-stage condensation -30℃; external second-stage condensation -40℃, and the distillation rate was 48.6%.

[0061] Example 2 (1) Mix 9 parts ginseng, 9 parts Atractylodes lancea, 1 part Acorus tatarinowii, 9 parts Perilla frutescens, 9 parts Pogostemon cablin, 6 parts Mentha haplocalyx, 9 parts Sandalwood, 9 parts Lonicera japonica, and 6 parts Glycyrrhiza uralensis to obtain a vehicle-mounted invigorating and fatigue-relieving traditional Chinese medicine composition; (2) Grind the traditional Chinese medicine composition using a grinder to obtain a 60-mesh powder; (3) Load the powder into a supercritical fluid extraction device and perform supercritical fluid extraction with CO2. The extraction parameters are: temperature 50℃, pressure 3 5MPa, extraction time 3h, flow rate 900L / h, to obtain primary extracted essential oil, extraction rate 3.08%; (4) Put the primary extracted essential oil into a molecular distillation purification device, and perform molecular distillation purification on the primary extracted essential oil to obtain car refresh and anti-fatigue fragrance essential oil; the molecular distillation purification parameters are: preheating temperature 80℃; evaporation temperature: 155℃; internal cooling 20℃; external first-stage condensation -30℃; external second-stage condensation -40℃, distillation rate 56.4%.

[0062] Example 3 (1) Mix 12 parts of ginseng, 12 parts of Atractylodes lancea, 3 parts of Acorus tatarinowii, 12 parts of Perilla frutescens, 12 parts of Pogostemon cablin, 2 parts of peppermint, 3 parts of sandalwood, 3 parts of honeysuckle, and 7 parts of licorice to obtain a vehicle-mounted invigorating and fatigue-relieving traditional Chinese medicine composition; (2) Grind the traditional Chinese medicine composition using a grinder to obtain a 60-mesh powder; (3) Load the powder into a supercritical fluid extraction device and perform supercritical fluid extraction with CO2. The extraction parameters are: temperature 50℃, pressure Force 35MPa, extraction time 3h, flow rate 900L / h, to obtain primary extracted essential oil, extraction rate 2.75%; (4) Put the primary extracted essential oil into a molecular distillation purification device, and perform molecular distillation purification on the primary extracted essential oil to obtain car refresh and anti-fatigue fragrance essential oil; the molecular distillation purification parameters are: preheating temperature 80℃; evaporation temperature: 155℃; internal cooling 20℃; external first-stage condensation -30℃; external second-stage condensation -40℃, distillation rate 51.5%.

[0063] The in-car invigorating and fatigue-relieving traditional Chinese medicine composition and in-car invigorating and fatigue-relieving aromatherapy essential oil provided in this application embodiment may contain volatile medicinal active ingredients. People can absorb the medicinal active ingredients in the in-car invigorating and fatigue-relieving traditional Chinese medicine composition through the respiratory tract or skin, experiencing a pleasant aroma while non-invasively absorbing the medicine. The in-car invigorating and fatigue-relieving traditional Chinese medicine composition regulates qi and blood, strengthens the spleen and eliminates dampness, and unblocks meridians, effectively relieving fatigue and refreshing the mind. The following specific experimental data illustrates some of the advantages of this application embodiment compared to the prior art.

[0064] I. Animal behavioral efficacy evaluation test (1) 1. Experimental animals and breeding environment: 1.1 Experimental animals; species: rat.

[0065] Strain: C57BL / 6 mice. Grade: SPF.

[0066] Quantity: 56 individuals. Weight: 20-25g. 1.2 Husbandry Environment: Room temperature: 22℃ 24℃. Relative humidity: 40%. 70%. Rearing conditions: Individually housed, with free access to food and water, constant temperature and humidity, and a stable day-night cycle (12 hours of light / 12 hours of darkness).

[0067] 2. Experimental Methods: Forty male C57BL / 6 mice (weighing 20-25g) were randomly divided into a blank group, a model group, Fragrance No. 1 group (Example 1), Fragrance No. 2 group (Example 2), and Fragrance No. 3 group (Example 3) using a random number table. The experiment began after 7 days of environmental acclimatization.

[0068] The control group was not provided with essential oils for mice to smell and was not subject to sleep deprivation. The model group was not provided with essential oils for mice to smell and was subject to sleep deprivation for 24 hours. The Fragrance Group 1 (Example 1), Fragrance Group 2 (Example 2), and Fragrance Group 3 (Example 3) were provided with essential oils for mice to smell for 24 hours, while sleep deprivation was maintained during the smelling process.

[0069] Animal model establishment: Mice subjected to sleep deprivation were treated with a DB036 SD instrument for 24 hours. The speed was set at 3.0 m / min, with a 10-second interval between movement and rest. During this process, each mouse was in an independent space and had free access to food and water.

[0070] 3. Open field test: The mice were placed in a square open field box with dimensions of 50cm×50cm×40cm. The bottom of the box was divided into 4 equal-area areas.

[0071] The behavioral parameters of the mice over 5 minutes were recorded: movement distance, movement speed, frequency of occurrence, and cumulative duration. Data were acquired using an intelligent video analysis system (EthoVision XT), and the results are shown in Figure 1. Simultaneously, infrared light was used to record the movement trajectory of the mice in the open field, and the results are shown in Figure 2.

[0072] As shown in Figures 1 and 2, the movement distance, speed, frequency of occurrence, and cumulative duration of mice in the model group (M) after sleep deprivation were significantly lower than those in the control group (N), and the movement trajectory of mice in the model group (M) was also significantly shorter than that in the control group (N). However, the movement distance, speed, frequency of occurrence, and cumulative duration of mice in the Fragrance Groups 1 (XF1), 2 (XF2), and 3 (XF3) were significantly higher than those in the model group (M). Furthermore, the movement trajectories of mice in the Fragrance Groups 1 (XF1), 2 (XF2), and 3 (XF3) were also increased compared to the model group (M). This demonstrates that after sleep deprivation combined with inhalation of the fragrance essential oil, the mice significantly regained their energy, indicating that the car-mounted refreshing and anti-fatigue fragrance essential oil prepared in this application can effectively relieve fatigue and refresh the mind.

[0073] Furthermore, comparing the performance of Fragrance Group 1 (XF1), Fragrance Group 2 (XF2), and Fragrance Group 3 (XF3), it was observed that the mice in Fragrance Group 2 (XF2) exhibited superior movement distance, speed, frequency of occurrence, and cumulative duration compared to Fragrance Group 1 (XF1) and Fragrance Group 3 (XF3). Additionally, the movement trajectory of the mice in Fragrance Group 2 (XF2) was also increased compared to Fragrance Group 1 (XF1) and Fragrance Group 3 (XF3). This indicates that Example 2, corresponding to Fragrance Group 2, is superior to Examples 1 and 3 in relieving fatigue and improving mental alertness.

[0074] II. Gas Chromatography-Mass Spectrometry Composition Analysis: The in-car refreshing and anti-fatigue aromatherapy essential oil obtained in Example 2 was analyzed using a gas chromatography-mass spectrometry (Agilent 8860-5977B) system.

[0075] Preparation of the test solution: Accurately transfer 1 ml of the car-mounted refreshing and anti-fatigue aromatherapy essential oil obtained in Example 2 into a 50 ml volumetric flask, dissolve and dilute to the mark with an appropriate amount of dichloromethane, and shake well; accurately pipette 0.5 ml of the solution from the volumetric flask, add dichloromethane to make up to a 10 ml volumetric flask, mix well, and the test solution is obtained.

[0076] Gas chromatography-mass spectrometry (GC-MS) analysis conditions: Column: J&W DB-5ms capillary column (30m×250μm×0.25μm); Carrier gas: helium; Split ratio: 10:1; Split flow rate: 10ml / min; Injection volume: 1μl; Flow rate: 1ml / min; Injector temperature: 300℃; Column temperature: programmed temperature rise (initial temperature 50℃, hold for 0min; increase to 120℃ at 10℃ / min, hold for 0min; increase to 210℃ at 3℃ / min, hold for 0min; increase to 320℃ at 10℃ / min, hold for 5min).

[0077] Ionization method: electron impact ionization (EI); ionization voltage: 70 eV; ion source temperature: 230℃; quadrupole temperature: 150℃; acquisition time: 4 min to 53 min; acquisition mode: full scan; scan range: m / z: 40 to 600.

[0078] After searching the Pharmacopoeia of the People's Republic of China (2020 Edition) (hereinafter referred to as the 20th Edition Pharmacopoeia) and Chinese and English literature, comparing with the NIST database, and combining the peak heights of relevant peaks in the total ion chromatogram (Figure 3), the following 16 possible components were obtained, as shown in Table 1.

[0079] Table 1. Possible components of car-use refreshing and fatigue-relieving aromatherapy essential oils

[0080] III. Gas Chromatography-Mass Spectrometry Analysis: The car-use refreshing and anti-fatigue aromatherapy essential oil obtained after molecular distillation in Example 2 was analyzed using a gas chromatograph (Agilent 7890A).

[0081] Gas chromatography (GC) analysis conditions: Column: Rtx-5 capillary column (30 m × 250 μm × 0.25 μm); Carrier gas: nitrogen; Split ratio: 10:1; Injection volume: 1 μl; Flow rate: 0.7 ml / min; Injector temperature: 300 °C; Column temperature: programmed temperature rise (initial temperature 50 °C, hold for 0 min; increase to 120 °C at 10 °C / min, hold for 0 min; increase to 180 °C at 3 °C / min, hold for 1 min; increase to 300 °C at 15 °C / min, hold for 5 min); Detector temperature: 310 °C.

[0082] Preparation of reference solutions: Accurately weigh appropriate amounts of menthol, elemol, dill oil, γ-cineole, atractylodes lancea, β-cineole, baicalein, and santalol (α / β) reference standards, and add dichloromethane to prepare mixed solutions containing 0.02 mg of menthol and 0.02 mg of elemol; 0.05 mg of dill oil and 0.05 mg of γ-cineole; 0.1 mg of atractylodes lancea, 0.1 mg of β-cineole, 0.15 mg of baicalein, 0.35 mg of α-santalol, and 0.15 mg of β-santalol per 1 ml.

[0083] Preparation of the test solution: Accurately pipette 0.1 ml of the car-use refreshing and anti-fatigue aromatherapy essential oil obtained after molecular distillation in Example 2 into a 50 ml volumetric flask, add dichloromethane solution to make up to volume, and prepare a mixed solution containing 0.002 ml of essential oil per 1 ml.

[0084] Assay: Accurately pipette 1 μl each of the above reference solution and test solution, inject them into the gas chromatograph, and measure. The resulting chromatogram is shown in Figure 3.

[0085] Based on the analysis of the possible components of the fragrance essential oil shown in Table 1 and the gas chromatogram shown in Figure 3, it can be seen that the car-mounted refreshing and anti-fatigue fragrance essential oil prepared in the embodiments of this application mainly contains menthol, 6-methyl-3-(1-methylethyl)-7-oxabicyclo[4.1.0]heptane-2-one, elemol, dill oil, γ-eucalyptol, atractylodes lancea alcohol, β-eucalyptol, baicalein, α-santalol, 9 (10) -Z-α-trans-limonene alcohol, epi-β-santalol, β-santalol, 2-methyl-5-[(1S,4R,6R)-6-methyl-5-methylene-6-bicyclo[2.2.1]heptyl]pent-2-en-1-ol, phytol, 7,9-di-tert-butyl-1-oxaspiro[4.5]dec-6,9-diene-2,8-dione, 2,3-dimethyl-5-(trifluoromethyl)-1,4-benzenediol, etc.

[0086] Multiple batches of car-invigorating and anti-fatigue fragrance oils were prepared using the same method as described in Example 2. The content of various components in each batch of car-invigorating and anti-fatigue fragrance oils was determined. Based on the specificity of each component, the content was calculated as a percentage of peak area (after removing solvent peaks). The determined content of menthol in the essential oil was 0.97-1.46%, elemol 2.08-3.12%, dill 0.15-0.22%, γ-eucalyptol 1.02-1.52%, atractylodes 4.81-7.21%, β-eucalyptol 10.49-15.74%, berberine 1.90-2.85%, α-santalol 11.19-16.78%, and 9 (10) The content of -Z-α-trans-limonene alcohol should be 1.57-2.36%, the content of epi-β-santalol should be 1.35-2.03%, and the content of β-santalol should be 5.23-7.84%.

[0087] The total content of the above components, calculated as a percentage of peak area in gas chromatography, should be between 40.77% and 61.15%.

[0088] IV. Animal behavioral efficacy evaluation test (2) 1. Experimental animals and breeding environment: 1.1 Experimental animals; species: rat.

[0089] Strain: C57BL / 6 mice. Grade: SPF.

[0090] Quantity: 56 individuals. Weight: 20-25g. 1.2 Husbandry Environment: Room temperature: 22℃ 24℃, relative humidity: 40% 70%. Rearing conditions: Separate cages, free access to food and water, constant temperature and humidity, maintaining a stable day-night cycle (12 hours of light / 12 hours of darkness).

[0091] 2. Experimental Methods: Forty male C57BL / 6 mice (weighing 20-25g) were randomly divided into four groups using a random number table: blank group, model group, competitor 1 control group, competitor 2 control group, low-concentration herbal fragrance group, medium-concentration herbal fragrance group, and high-concentration herbal fragrance group. The experiment began after 7 days of environmental acclimatization.

[0092] In the control group, mice were not provided with essential oils to smell and were not deprived of sleep, while mice in the model group were not provided with essential oils to smell and were deprived of sleep for 24 hours.

[0093] The control group for competitor product 1 was fragrance block A from a certain brand, and the control group for competitor product 2 was fragrance block B from a certain brand. Fragrance blocks A and B have different formulas.

[0094] The low-concentration group of traditional Chinese medicine fragrances consisted of a fragrance liquid containing 15% of the car-invigorating and anti-fatigue fragrance essential oil from Example 2; the medium-concentration group of traditional Chinese medicine fragrances consisted of a fragrance liquid containing 22.5% of the car-invigorating and anti-fatigue fragrance essential oil from Example 2; and the high-concentration group of traditional Chinese medicine fragrances consisted of a fragrance liquid containing 33.75% of the car-invigorating and anti-fatigue fragrance essential oil from Example 2.

[0095] Mice were given samples to smell for 24 hours in each of the following groups: the competitor 1 control group, the competitor 2 control group, the low concentration group of traditional Chinese medicine fragrance, the medium concentration group of traditional Chinese medicine fragrance, and the high concentration group of traditional Chinese medicine fragrance. Sleep deprivation was maintained during the smelling process.

[0096] Animal model establishment: Mice subjected to sleep deprivation were treated with a DB036 SD instrument for 24 hours. The speed was set at 3.0 m / min, with a 10-second interval between movement and rest. During this process, each mouse was in an independent space and had free access to food and water.

[0097] 3. Open field test: The mice were placed in a square open field box with dimensions of 50cm×50cm×40cm. The bottom of the box was divided into 4 equal-area areas.

[0098] The behavioral parameters of the mice over 5 minutes were recorded: movement distance, movement speed, frequency of occurrence, and cumulative duration. Data were acquired using an intelligent video analysis system (EthoVision XT), and the results are shown in Figure 5. Simultaneously, infrared light was used to record the movement trajectory of the mice in the open field, and the results are shown in Figure 6.

[0099] In the figure, ns, *, **, ***, and **** are statistical significance markers, representing the degree of difference in the p-value (probability value) between the two groups. ns represents P > 0.05, indicating no significant difference between the two groups. * represents P < 0.05, indicating a significant difference between the two groups. ** represents P < 0.01, indicating a highly significant difference between the two groups. *** represents P < 0.001, indicating an extremely significant difference between the two groups. **** represents P < 0.0001, indicating a highly significant difference between the two groups.

[0100] As shown in Figure 5, after sleep deprivation, the frequency and cumulative time of the central analysis area in the model group (M) mice were significantly reduced compared with the blank group (N) (P<0.001). There was no significant improvement in the competitor 1 control group (J1) and competitor 2 control group (J2). The low concentration group (XL), medium concentration group (XM), and high concentration group (XH) of traditional Chinese medicine fragrance were improved compared with the previous results (P<0.001), and showed a dose-dependent increase.

[0101] As shown in Figures 5 and 6, the model group (M) mice also showed a significant decrease in movement speed and distance in the open field (P<0.001). The competitor 1 control group (J1) and competitor 2 control group (J2) showed no significant improvement. The low concentration group (XL), medium concentration group (XM), and high concentration group (XH) of traditional Chinese medicine fragrance showed improvement compared to the previous group (P<0.001), and the increase was dose-dependent.

[0102] 4. Elevated Cross Maze Test: After the open field test, mice from each group were placed in the elevated cross maze apparatus, facing away from the experimenters, in the central area of ​​the maze, facing the open arms. The number of times the mice entered the open and closed arms, and the time spent in each arm were observed and recorded to evaluate the effect of the fragrance on improving neurological and anti-anxiety functions in mice. The results are shown in Figure 7. Simultaneously, infrared light was used to record the mice's movement trajectory, and the results are shown in Figure 8.

[0103] As shown in Figure 7, the frequency of open arms in the model group (M) mice was significantly reduced compared with the blank group (N) (P<0.001). Compared with the model group (M), the competition product 1 control group (J1) and the competition product 2 control group (J2) showed partial improvement (P<0.05), and the low concentration group (XL), medium concentration group (XM), and high concentration group (XH) of traditional Chinese medicine fragrance showed significant improvement (P<0.001).

[0104] The frequency of mice in the model group (M) in the closed arm was significantly reduced (P<0.001). Compared with the model group (M), there was no significant improvement in the competitor 1 control group (J1) and competitor 2 control group (J2). The low concentration group (XL), medium concentration group (XM), and high concentration group (XH) of traditional Chinese medicine fragrance were improved (P<0.001) and showed a dose-dependent increase.

[0105] As shown in Figure 8, the exploration desire of mice in the model group (M) was significantly reduced. There was no significant improvement in the control groups of competitor product 1 (J1) and competitor product 2 (J2). The low concentration group (XL), medium concentration group (XM), and high concentration group (XH) of traditional Chinese medicine fragrance were improved compared with the previous results and showed a dose-dependent increase.

[0106] 5. Tail Suspension Test: After the elevated cross test, mice in each group were gently secured to the tail suspension test apparatus with medical tape approximately 1 cm from the tip of their tails. The mice's struggling behavior was observed and recorded, including attempts to escape, limb swinging, and the final immobile state. The immobility time during the last 4 minutes was analyzed in detail. The effects of the fragrance on improving neurological function and antidepressant function in mice were evaluated; the results are shown in Figure 9.

[0107] As shown in Figure 9, after sleep deprivation, the suspension time of mice in the model group (M) was significantly increased compared with that in the blank group (N) (P<0.001). Compared with the model group (M), the control group of competitor 1 (J1) showed no significant improvement, the control group of competitor 2 (J2) showed slight improvement (P<0.01), and the low concentration group (XL), medium concentration group (XM), and high concentration group (XH) of traditional Chinese medicine fragrance showed significant improvement (P<0.001).

[0108] The model group (M) mice had significantly reduced suspension struggle time (P<0.001), the competitor 1 control group (J1) showed no significant improvement, the competitor 2 control group (J2) showed slight improvement (P<0.01), and the low concentration group (XL), medium concentration group (XM), and high concentration group (XH) of traditional Chinese medicine fragrance showed significant improvement (P<0.001).

[0109] 6. Time gradient test to test the changes in the exploration desire and behavioral activity of mice under the administration time of 3, 6, 12 and 24 hours for the competitor product 1 control group, competitor product 2 control group, low concentration group of traditional Chinese medicine fragrance, medium concentration group of traditional Chinese medicine fragrance and high concentration group of traditional Chinese medicine fragrance.

[0110] During the 3-hour administration period, mice were simultaneously deprived of sleep, followed by a 21-hour sleep deprivation.

[0111] During the 6-hour administration period, mice were simultaneously deprived of sleep, followed by an 18-hour sleep deprivation.

[0112] During the 12-hour administration period, mice were simultaneously deprived of sleep, followed by another 12-hour period of sleep deprivation.

[0113] During the 24-hour administration period, mice were simultaneously deprived of sleep.

[0114] Subsequently, the behavioral parameters of the mice were tested using the same method as in the open field experiment, and the distances the mice moved at 3, 6, 12, and 24 hours after administration were recorded, as shown in Figure 10.

[0115] As shown in Figure 10, 3 hours after administration, the high-concentration group of the traditional Chinese medicine fragrance promoted the mice's exploratory desire and behavioral activity (P<0.001), while the control groups of competitor product 1 and competitor product 2 showed no significant improvement. The low-concentration and medium-concentration groups of the traditional Chinese medicine fragrance showed improvement, but it was not significant. The fragrances in the low-concentration, medium-concentration, and high-concentration groups of the traditional Chinese medicine fragrance all had a refreshing and anti-fatigue function, which was more pronounced at higher concentrations and more effective with longer application time.

[0116] 7. Blood was collected from mice for biochemical experiments to test their liver function, kidney function, and antioxidant function. The results are shown in Figure 11.

[0117] The neurotransmitter content in the brain tissue of mice was tested, and the results are shown in Figure 12.

[0118] Brain tissue sections were collected from mice, fixed, dehydrated, embedded, and sectioned. The sections were then stained with hematoxylin and eosin (HE) to observe the morphological changes in the brain tissue. The results are shown in Figure 13.

[0119] As shown in Figure 11, the liver function, kidney function, and antioxidant function of the control group (N) mice were all within the normal range. The liver function of the model group (M) mice, including ALT (alanine aminotransferase), AST (aspartate aminotransferase), and GGT (gamma-glutamyl transferase), was significantly elevated (P<0.01). Compared with the model group (M), the liver function of the low-concentration group (XL), medium-concentration group (XM), and high-concentration group (XH) of the herbal fragrance was significantly improved (P<0.01). The liver function of the competitor 1 control group (J1) and competitor 2 control group (J2) was also improved compared with the model group (M), but their effect on improving liver function in mice was not as significant as that of the low-concentration group (XL), medium-concentration group (XM), and high-concentration group (XH) of the herbal fragrance.

[0120] In the model group (M) mice, renal function CREA (creatinine) and BUN (blood urea nitrogen) were significantly elevated (P<0.001). Compared with the model group (M), renal function was significantly improved in the low-concentration group (XL), medium-concentration group (XM), and high-concentration group (XH) of the herbal fragrance (P<0.001). The liver function of the competitor 1 control group (J1) and competitor 2 control group (J2) was also improved compared with the model group (M), but their effect on improving renal function in mice was not as significant as that in the low-concentration group (XL), medium-concentration group (XM), and high-concentration group (XH) of the herbal fragrance.

[0121] The reduction of oxidative stress damage was observed in the model group (M) mice, with significantly decreased levels of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) (P<0.001). Compared with the model group (M), the antioxidant functions of the low-concentration (XL), medium-concentration (XM), and high-concentration (XH) herbal fragrance groups were significantly improved (P<0.05; P<0.001), showing a dose-dependent increase. The liver function of the competitor 1 control group (J1) and competitor 2 control group (J2) was also improved compared with the model group (M), but their effect on improving the antioxidant function of mice was not as significant as that of the low-concentration (XL), medium-concentration (XM), and high-concentration (XH) herbal fragrance groups.

[0122] As shown in Figure 12, the neurological function of mice in the model group (M) was significantly reduced by DA (glutamate) and Glu (dopamine) compared to the blank group (N) (P<0.001). Compared with the model group (M), the control groups of competitor product 1 (J1) and competitor product 2 (J2) showed no significant improvement. The neurological function of mice in the low-concentration group (XL), medium-concentration group (XM), and high-concentration group (XH) of traditional Chinese medicine fragrance was significantly improved (P<0.01; P<0.001), showing a dose-dependent increase. The 5-HT (serotonin) level of mice in the model group (M) was significantly increased (P<0.001). Compared with the model group (M), the 5-HT levels of mice in the low-concentration group (XL), medium-concentration group (XM), and high-concentration group (XH) of traditional Chinese medicine fragrance were significantly improved (P<0.001).

[0123] As shown in Figure 13, the blank group (N) consisted of normal mice with no abnormalities in brain tissue structure. The neurons in the hippocampus were neatly and tightly arranged in the field of vision, without degeneration or necrosis; no obvious inflammatory cell infiltration was observed in the tissue.

[0124] The brain tissue of the model group (M) mice was severely abnormal, with a large number of neuronal cells degenerated, staining darker, and no clear cell bodies were observed.

[0125] The brain tissue structures of the competitor 1 control group (J1) and the competitor 2 control group (J2) were significantly abnormal, with many neuronal cells showing degeneration and darkening of staining, and no clear cell bodies were observed.

[0126] The brain tissue structure of the low-concentration group (XL) of traditional Chinese medicine fragrance was improved compared with that of the model group (M), with some neurons showing degeneration and darker staining, and no clear cell bodies were observed. The brain tissue structure of the medium-concentration group (XM) and the high-concentration group (XH) of traditional Chinese medicine fragrance was significantly improved.

[0127] As shown in Figure 13, sleep deprivation-induced fatigue in mice causes damage to neurons in the hippocampus. The competitor's 1 control group and competitor's 2 control group failed to effectively improve the neuronal damage caused by fatigue in mice. The low-concentration group (XL) of the traditional Chinese medicine fragrance slightly improved the neuronal damage caused by fatigue in mice. The medium-concentration group (XM) and the high-concentration group (XH) of the traditional Chinese medicine fragrance effectively improved the neuronal damage caused by fatigue in mice.

[0128] In summary, the in-car refreshing and anti-fatigue aromatherapy essential oil provided in this application embodiment can effectively relieve fatigue and refresh the mind by regulating qi and blood, strengthening the spleen and removing dampness, and unblocking meridians through the traditional Chinese medicine composition.

[0129] V. Clinical Trial 1. Study Content 1.1 Assessment of enrolled patients and diagnostic criteria for chronic fatigue syndrome

[0130] 1.1.1 The diagnostic criteria for Western medicine refer to the diagnostic criteria for chronic fatigue syndrome revised by the Centers for Disease Control and Prevention (CDC): (1) Unexplained persistent severe fatigue lasting for 6 months or more, fatigue symptoms cannot be relieved even with adequate rest, and activity level is ≥50% lower than when healthy; (2) At the same time, four or more of the following symptoms must be present for a diagnosis of CFS to be established: ① memory loss; ② sleep disorder; ③ persistent discomfort after exertion for more than 24 hours.

[0131] (3) Symptomatic chronic fatigue needs to be excluded: ① The presence of the primary disease can explain chronic fatigue. For example: undiagnosed and untreated hypothyroidism, iatrogenic fatigue; ② Diseases that are difficult to treat under current medical conditions and can cause chronic fatigue, such as patients with hepatitis B or hepatitis C; ③ Patients with a history or current diagnosis of depressive mood disorder, bipolar mood disorder with depressive features, various types of schizophrenia, dementia, delusions, anorexia nervosa or bulimia nervosa; ④ Bad habits from 2 years before the onset of the disease to the present, such as smoking, alcoholism, etc.; ⑤ Severe obesity (BMI index > 45).

[0132] 1.1.2 The diagnostic criteria of TCM refer to the relevant content and research of the textbook "Internal Medicine of Traditional Chinese Medicine" published by People's Medical Publishing House in 2012 and edited by Zhang Boli and Xue Boyu; "Encyclopedia of Chinese Massage" published by People's Medical Publishing House in 2009 and edited by Luo Zhongyao; and "Acupuncture" published by China Traditional Chinese Medicine Publishing House in 2002 and edited by Shi Xuemin as the planning textbook for the new century of national higher TCM colleges: (1) No obvious organic lesions of organs, mainly characterized by deficiency fatigue; (2) Mainly characterized by lethargy, fatigue, insomnia and forgetfulness; (3) Often accompanied by related symptoms of deficiency of organs, qi and blood, yin and yang. If two or more of the following main symptoms and two or more of the following secondary symptoms are met, the diagnosis is this disease.

[0133] Main symptoms: fatigue, shortness of breath, reluctance to speak, restless sleep, dizziness and heaviness in the head.

[0134] Secondary symptoms: sallow or pale complexion, spontaneous sweating, low voice, poor appetite, and loose stools.

[0135] Tongue and pulse: The tongue is pale and swollen, and the pulse is thin or weak.

[0136] 1.2 Inclusion, Exclusion, Withdrawal and Termination Criteria 1.2.1 Inclusion Criteria (1) Age between 18 and 60 years (inclusive), gender not limited; (2) Meets the diagnostic criteria for chronic fatigue syndrome; (3) MFI score > 60 points; (4) No abnormalities in blood routine, heart, liver and kidney function, echocardiography, coagulation panel, electrocardiogram and other indicators; (5) Willing to accept the observation of this study plan, with good compliance, clear thinking, clear expression, and normal language and sensory response; (6) Able to understand the situation of this study and sign the informed consent form, and willing to cooperate with the follow-up.

[0137] 1.3.2 Exclusion criteria (1) Accompanied by serious diseases of other systems such as heart, kidney, endocrine or other parts of the body, or tumors in other parts of the body; (2) Pregnant or lactating women; (3) Those who are allergic to known prescription ingredients or have a severe allergic constitution; (4) Those who are currently participating in or have participated in other drug clinical trials within 4 weeks.

[0138] 1.2.3 Exclusion criteria (1) Cases that were mistakenly included but did not meet the inclusion criteria, and those who withdrew from the trial after being included but met the inclusion criteria; (2) Patients who received other treatments related to the disease during the treatment process, or patients in the control group who received the treatments in the experimental group, were excluded to avoid interference and contamination; (3) Subjects with poor compliance who could not receive treatment as required, affecting the judgment of efficacy; (4) Subjects with incomplete data that affected the judgment of efficacy; (5) Subjects who requested to withdraw from the clinical trial or change medication midway due to ineffectiveness or poor efficacy; (6) Subjects who withdrew midway or were lost to follow-up due to other reasons.

[0139] 1.3.4 Termination Criteria (1) If a serious physical illness is found after enrollment, the trial will be terminated to avoid delaying treatment; (2) If the patient has poor compliance, fails to have a timely follow-up visit, or is unable to cooperate with treatment, which may cause the condition to worsen or if the condition progresses rapidly during treatment, the trial will be terminated; (3) If a serious adverse reaction occurs during treatment, the trial will be terminated; (4) If the patient refuses to continue cooperating with treatment for various reasons.

[0140] 1.3 Treatment methods 1.3.1 Control group The control group was given fragrance blocks of the competing product 1 (J1).

[0141] 1.3.2 Experimental Group The experimental group was prepared with the in-car refreshing and anti-fatigue aromatherapy essential oil obtained in Example 2.

[0142] 1.3.3 Treatment method: Inhale the fragrance for 30 minutes, repeat three times.

[0143] 1.4 Sample Size Determination Based on the minimum effective sample size of Phase I clinical trials, the sample size of this trial was designed to be 60 cases, with 30 cases in the control group and 30 cases in the experimental group.

[0144] 1.5 Grouping Method: Block randomization was performed using SAS 9.3, with a 1:1 ratio between each group. The statistical software generated a random number table, and the subjects were allocated to each group in a 1:1 ratio.

[0145] 1.6 General Information Collection General information includes name, gender, age, height, weight, marital status, occupation, past medical history, family history, years of alcohol consumption, alcohol alcohol content, comorbidities, medical history, course of illness, treatment history, comorbidities and medications, urine HCG test (for women), etc.

[0146] 1.7 Information Collection from the Four Diagnostic Methods of Traditional Chinese Medicine To facilitate the recording of patients' clinical symptoms, signs, and tongue and pulse examination information, this study used the Four Diagnostic Methods Information Collection Scale for Asthenia. In addition to including frequently occurring symptoms and signs, it also retained less frequently occurring symptoms and signs that are clinically significant for disease differentiation, and adjusted the semi-quantitative standards for symptoms. In clinical practice, the occurrence of symptoms is not only manifested in differences in frequency or timing, but also often in differences in the severity of the symptoms at the time of occurrence. Therefore, based on the clinical characteristics and symptom characteristics of asthenia itself, scales were developed to separately define the frequency of symptom and sign occurrence and the severity of symptoms at the time of occurrence. Since there are significant differences in tongue and pulse examination between different types of asthenia, tongue and pulse examination data collection was added to the scale to more fully reflect the collection of information from the four diagnostic methods, which is consistent with the theoretical basis of traditional Chinese medicine diagnosis and treatment. The order of items was randomized to break the respondents' preconceived notions; to facilitate the differentiation of easily confused questions, easily confused questions were placed in adjacent positions.

[0147] 1.8 Observation Indicators Observation indicators: (1) Baseline indicators: including age, gender, height, weight and other indicators; (2) Scales: ① Use the scales commonly used in clinical research at home and abroad to evaluate the severity of chronic fatigue syndrome from different perspectives. The Multidimensional Fatigue Inventory (MFI-20) was used; ② Refer to the chronic fatigue syndrome symptom scoring scale in the 2002 Guidelines for Clinical Research of New Traditional Chinese Medicines; (3) Laboratory tests: blood routine, urine routine, stool routine, liver and kidney function + blood glucose; (4) Electrocardiogram examination.

[0148] 1.9 Efficacy Assessment 1.9.1 MFI-20 Questionnaire The subjective fatigue status of the subjects was scientifically assessed based on the MFI-20 questionnaire.

[0149] 1.9.2 Evaluation Criteria for TCM Syndrome Therapy: All symptoms are divided into four levels: none, mild, moderate, and severe. Primary symptoms are scored as 0, 2, 4, and 6 points respectively, while secondary symptoms are scored as 0, 1, 2, and 3 points respectively. The efficacy index = (pre-treatment score - post-treatment score) / pre-treatment score × 100%.

[0150] Effective: The main symptoms and signs are significantly improved, and the efficacy index is 30% ≤. Ineffective: The main symptoms and signs are not significantly improved, or even worsened, and the efficacy index is <30%. 1.10 Safety evaluation (1) Grade 1: No adverse reactions, and no abnormalities in safety indicators; (2) Grade 2: Relatively safe, and no abnormalities in safety indicators; mild adverse reactions, but can continue to receive treatment without any treatment; (3) Grade 3: Mild abnormalities in safety indicators, moderate adverse reactions, and can continue to receive treatment after treatment; (4) Grade 4: Serious adverse reactions or obvious abnormalities in safety indicators, and the study needs to be terminated.

[0151] 1.11 Specimen Collection: Blood collection: Collect 5 ml each of procoagulant and EDTA anticoagulant blood from the patient before and after treatment. Centrifuge the procoagulant blood at 2500 rpm for 10 min, and collect the serum for later use at -80 degrees Celsius. Separate 500 μL of whole blood from the anticoagulant blood and store it at -80 degrees Celsius for later testing. Centrifuge the remaining whole blood at 2500 rpm for 10 min, and collect the plasma for later testing at -80 degrees Celsius.

[0152] Urine collection: A urine collection tube was provided to the patient upon their initial visit, and they were instructed to collect 10 ml of midstream morning urine at their next visit. All urine samples were centrifuged at 4°C, 3000 rpm for 15 min, and the supernatant was collected and stored at -80°C for later analysis.

[0153] 2. Statistical Methods: Excel 1 software was used to input and establish the database. SPSS 20.0 statistical software was used for statistical analysis to describe baseline data such as gender, age, education level, and disease duration. Count data were expressed as rates and proportions, and chi-square tests were used between count data. Normally distributed measurement data were expressed as two-sample means ± standard deviation (x±S), and t-tests were used. For comparisons of data that did not conform to a normal distribution, paired rank-sum tests were used for comparisons within a group, and independent rank-sum tests were used for comparisons between groups. The significance level α was set at 0.05, and 95% confidence intervals were used for parameter estimation. Hypothesis testing was uniformly performed using two-tailed tests, with P<0.05 considered statistically significant and P<0.01 considered highly statistically significant.

[0154] Statistical analysis was performed on the baseline data of 60 patients who completed the clinical study, including basic patient information such as age, gender, complete blood count, liver function, kidney function, blood lipids, blood glucose levels, and other laboratory biochemical indicators, as shown in Table 2.

[0155] Table 2 Baseline Characteristics of Cases

[0156] As can be seen, the mean age of the control group was 31.30±6.89 years, and that of the experimental group was 32.97±7.20 years, with no statistically significant difference in age distribution between the two groups (P=0.364). The male-to-female ratio in the control group was 11:19 (11 males and 19 females), and that in the experimental group was 12:18 (12 males and 18 females), with no significant difference in gender ratio between the two groups (P=0.791).

[0157] In the laboratory parameters, there were no significant differences between the control and experimental groups in the following blood routine parameters: WBC (white blood cell count) (5.58±1.29 vs. 5.23±1.02, P=0.486), RBC (red blood cell count) (4.57±0.65 vs. 4.46±0.80, P=0.566), PLT (platelet count) (203.67±63.48 vs. 196.43±63.31, P=0.930), and HGB (hemoglobin) (151.53±12.76 vs. 154.07±11.90, P=0.134).

[0158] There were no statistically significant differences in any of the liver function indicators, including ALT (alanine aminotransferase) (28.07±10.43 vs. 31.10±10.31, P=0.566), AST (aspartate aminotransferase) (29.20±7.69 vs. 26.13±7.35, P=0.174), GGT (gamma-glutamyl transferase) (41.30±11.63 vs. 36.73±13.79, P=0.239), and TBIL (total bilirubin) (12.87±4.60 vs. 14.97±6.17, P=0.149).

[0159] There were no significant differences in renal function-related indicators such as BUN (blood urea nitrogen) (5.05±1.44 vs. 5.19±1.16, P=0.847), CR (creatinine) (80.23±11.21 vs. 79.37±11.71, P=0.450), and GFR (glomerular filtration rate) (103.40±7.12 vs. 101.50±5.69, P=0.117) between the groups.

[0160] Among the blood lipid and blood glucose indicators, TG (triglycerides) (1.46±0.32 vs. 1.30±0.37, P=0.058) and HbA1c (glycated hemoglobin) (5.55±0.88 vs. 5.93±1.00, P=0.098) showed nearly statistically significant differences, while other indicators such as TC (total cholesterol), HDL (high-density lipoprotein cholesterol), LDL (low-density lipoprotein cholesterol), and PG (blood glucose) showed no statistically significant differences (P>0.05).

[0161] The efficacy of the control group and the experimental group was compared and analyzed according to the MFI-20 scoring criteria. The results are shown in Table 3: Table 3 Comparison of Efficacy Based on MFI-20 Scores

[0162] As can be seen, before treatment, the baseline MFI-20 score was 86.43±6.06 in the control group and 85.37±6.12 in the experimental group. There was no significant difference in the scores between the two groups before treatment (P=0.501).

[0163] In the post-treatment efficacy analysis, the MFI-20 score in the control group decreased slightly to 83.27±8.72 after treatment, with no statistically significant difference compared to before treatment (P=0.130). In the experimental group, the score decreased significantly to 60.27±13.24 after treatment (from 85.37±6.12 before treatment), a highly statistically significant difference (P<0.001).

[0164] The comparison of efficacy between the two groups after treatment showed a significant difference in MFI-20 scores (P < 0.001), indicating that the MFI-20 score improvement in the experimental group was significantly better than that in the control group, with a significant decrease in scores after treatment (P < 0.001), while there was no significant change in the control group (P = 0.130).

[0165] The therapeutic effects of the control group and the experimental group were compared and analyzed according to the TCM syndrome scoring system. The results are shown in Table 4.

[0166] Table 4 Comparison of therapeutic effects based on TCM syndrome scores

[0167] Before treatment, the baseline scores of the control group were 32.33±3.38 and those of the experimental group were 32.40±4.48. There was no significant difference in the scores between the two groups before treatment (P=0.948).

[0168] In the post-treatment efficacy analysis, the control group's score decreased slightly to 31.60±6.15 after treatment, with no statistically significant difference compared to before treatment (P=0.542). The experimental group's score decreased significantly to 18.40±7.52 after treatment (from 32.40±4.48 before treatment), with a highly statistically significant difference (P<0.001).

[0169] The comparison of efficacy between the two groups after treatment showed a significant difference in TCM syndrome scores (P < 0.001), indicating that the improvement in scores in the experimental group was significantly better than that in the control group.

[0170] According to the TCM syndrome differentiation and efficacy evaluation criteria, the efficacy was divided into four levels: cured (symptom score improvement ≥95%), significantly effective (symptom score improvement ≥70%), effective (70% > symptom score improvement ≥30%), and ineffective (symptom score improvement <30%). The statistical results are shown in Table 5.

[0171] Table 5 Comparison of Efficacy Rates of Treatment Based on Syndrome Differentiation

[0172] As can be seen, in the control group of 30 patients, 11 cases (36.37%) were effective and 19 cases were ineffective; in the experimental group of 30 patients, 23 cases (76.67%) were effective and 7 cases were ineffective. The inter-group comparison showed that the total effective rate in the experimental group was significantly higher than that in the control group (76.67% vs. 36.37%), with a highly statistically significant difference (P < 0.001). Further analysis revealed that the improvement in TCM syndrome scores and the total effective rate in the experimental group were significantly better than those in the control group after treatment (P < 0.001), suggesting that the car-mounted refreshing and anti-fatigue aromatherapy essential oil provided in this application has a clear clinical advantage in alleviating TCM syndromes.

[0173] In summary, the experimental group, namely the car-mounted refreshing and anti-fatigue aromatherapy essential oil provided in this application embodiment, can significantly improve the fatigue symptoms (MFI-20 score) and TCM syndrome scores of patients with chronic fatigue syndrome, and the total effective rate is significantly better than that of the control group.

[0174] The above provides a detailed description of a vehicle-mounted refreshing and fatigue-relieving traditional Chinese medicine composition, aromatherapy essential oil, and its preparation method provided in this application. Specific examples have been used to illustrate the principles and implementation methods of this application. The descriptions of the above embodiments are only for the purpose of helping to understand the methods and core ideas of this application. At the same time, for those skilled in the art, there will be changes in the specific implementation methods and application scope based on the ideas of this application. Therefore, the content of this specification should not be construed as a limitation of this application.

Claims

1. A vehicle-mounted, energizing, and fatigue-relieving traditional Chinese medicine composition, characterized in that, The in-vehicle energizing and fatigue-relieving traditional Chinese medicine composition includes 1-12 parts of ginseng, 1-12 parts of Atractylodes lancea, 1-3 parts of Acorus tatarinowii, 1-12 parts of Perilla frutescens, 1-12 parts of Pogostemon cablin, 1-12 parts of Mentha haplocalyx, 1-12 parts of Sandalwood, 1-12 parts of Lonicera japonica, and 1-12 parts of Glycyrrhiza uralensis.

2. The in-vehicle energizing and fatigue-relieving traditional Chinese medicine composition according to claim 1, characterized in that, The vehicle-mounted invigorating and fatigue-relieving traditional Chinese medicine composition is used to relieve fatigue and refresh the mind. It includes 3-12 parts of ginseng, 3-12 parts of Atractylodes lancea, 1-3 parts of Acorus tatarinowii, 3-12 parts of Perilla frutescens, 3-12 parts of Pogostemon cablin, 2-7 parts of peppermint, 3-12 parts of sandalwood, 3-12 parts of honeysuckle, and 2-7 parts of licorice.

3. A car-mounted invigorating and fatigue-relieving aromatherapy essential oil, characterized in that, The in-car invigorating and fatigue-relieving aromatherapy essential oil includes the essential oil prepared from the in-car invigorating and fatigue-relieving traditional Chinese medicine composition as described in claim 1 or 2.

4. A method for preparing a car-mounted refreshing and anti-fatigue aromatherapy essential oil as described in claim 3, characterized in that, The method includes: mixing 1-12 parts of ginseng, 1-12 parts of Atractylodes lancea, 1-3 parts of Acorus tatarinowii, 1-12 parts of Perilla frutescens, 1-12 parts of Pogostemon cablin, 1-12 parts of Mentha haplocalyx, 1-12 parts of Sandalwood, 1-12 parts of Lonicera japonica, and 1-12 parts of Glycyrrhiza uralensis to obtain a car-mounted invigorating and fatigue-relieving traditional Chinese medicine composition; loading the car-mounted invigorating and fatigue-relieving traditional Chinese medicine composition into a supercritical fluid extraction device to perform supercritical fluid extraction on the car-mounted invigorating and fatigue-relieving traditional Chinese medicine composition to obtain a primary extract essential oil; loading the primary extract essential oil into a molecular distillation purification device to perform molecular distillation purification on the primary extract essential oil to obtain a car-mounted invigorating and fatigue-relieving aromatherapy essential oil.

5. The method according to claim 4, characterized in that, The step of loading the vehicle-mounted energizing and fatigue-relieving traditional Chinese medicine composition into a supercritical fluid extraction device and performing supercritical fluid extraction on the vehicle-mounted energizing and fatigue-relieving traditional Chinese medicine composition to obtain a preliminary extracted essential oil includes: grinding the vehicle-mounted energizing and fatigue-relieving traditional Chinese medicine composition to obtain a traditional Chinese medicine composition powder of 40-70 mesh; loading the traditional Chinese medicine composition powder into a supercritical fluid extraction device and performing supercritical fluid extraction on the traditional Chinese medicine composition powder to obtain a preliminary extracted essential oil.

6. The method according to claim 4 or 5, characterized in that, The supercritical fluid extraction satisfies at least one of the following: the extractant of the supercritical fluid extraction is carbon dioxide; the temperature range of the supercritical fluid extraction is 40℃~50℃; the pressure range of the supercritical fluid extraction is 15MPa~35MPa; the extraction time of the supercritical fluid extraction is 1h~3h; and the flow rate of the supercritical fluid extraction is 300L / h~900L / h.

7. The method according to claim 4, characterized in that, The molecular distillation purification apparatus includes a preheater, an evaporator, an external primary condenser, and an external secondary condenser; the evaporator is equipped with an internal condenser. The step of loading the primary extracted essential oil into the molecular distillation purification apparatus and performing molecular distillation purification on the primary extracted essential oil to obtain a car-use refreshing and anti-fatigue fragrance essential oil includes: passing the primary extracted essential oil into the preheater for preheating; passing the preheated primary extracted essential oil into the evaporator for evaporation, and separating and purifying the essential oil components in the primary extracted essential oil through the internal condenser to obtain the car-use refreshing and anti-fatigue fragrance essential oil; and passing the car-use refreshing and anti-fatigue fragrance essential oil sequentially through the external primary condenser and the external secondary condenser to obtain a cooled car-use refreshing and anti-fatigue fragrance essential oil.

8. The method according to claim 7, characterized in that, The molecular distillation purification satisfies at least one of the following: the preheating temperature of the preheater is 60℃~80℃; the evaporation temperature of the evaporator is 155℃~180℃; the internal condensation temperature of the internal condenser is 20℃~25℃; the external primary condensation temperature of the external primary condenser is -22℃~-30℃; and the external secondary condensation temperature of the external secondary condenser is -40℃~-78℃.

9. The method according to claim 4, characterized in that, The initial extraction rate of the supercritical fluid extraction essential oil is 2%~4%, and the distillation rate of the car-invigorating and anti-fatigue fragrance essential oil purified by molecular distillation is 48%~57%.

10. An application of a car-mounted invigorating and fatigue-relieving aromatherapy essential oil, characterized in that, The car-mounted refreshing and anti-fatigue aromatherapy essential oil as described in claim 3, or the car-mounted refreshing and anti-fatigue aromatherapy essential oil prepared by the method described in any one of claims 4 to 9, is used to relieve fatigue and refresh the mind.

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