Healthcare wine for maggot cultured based on fresh fish fermented feed and preparation method of healthcare wine

Abstract

The invention discloses a health care wine based on maggot cultured by a fresh fish fermented feed and a preparation method, the health care wine is composed of a base wine, a maggot extract, a traditional Chinese medicinal material composition and auxiliary materials, maggot is cultured by a feed obtained by fermenting fresh fish with a compound microbial agent, and then the maggot extract is prepared through cleaning, sterilization, freeze-drying and base wine reflux extraction; the traditional Chinese medicine composition is prepared from rhizoma polygonati, fructus lycii, mulberries, herba cistanche and radix morindae officinalis, a traditional Chinese medicine extracting solution is obtained through infiltration, decoction and concentration, finally, the base wine, the maggot extract, the traditional Chinese medicine extracting solution and the auxiliary materials are sealed, aged, filtered and filled, and a finished product is obtained. The fresh fish fermented feed is used for breeding maggots, potential safety hazards such as pathogenic bacteria and heavy metal brought by traditional feed are avoided from the source, nutritional ingredients of the maggots are improved, then the maggots are matched with traditional Chinese medicinal materials, synergistic gain of animal active ingredients and plant essence is achieved, and the product is rich in nutrition and easy to absorb; the traditional Chinese medicine composition has the effects of supporting healthy energy, strengthening tendons and bones and enhancing immunity.

Description

A health wine made from grain insects raised on fermented fresh fish feed and its preparation method Technical Field

[0001] This invention relates to the field of health wine technology, specifically to a health wine made from grain insects raised on fermented fresh fish feed and its preparation method. Background Technology

[0002] With the booming development of the health industry, insect-derived health foods rich in high-quality protein and specific physiologically active substances have gradually become a market hotspot. Grain worms, as traditional medicinal insects, are rich in protein, chitin, and various trace elements. In traditional Chinese medicine, they are believed to have effects such as strengthening the spleen, resolving stagnation, clearing the lungs, and relieving coughs, and have broad application potential in the health food field. However, existing technologies have many problems. First, traditional grain worm farming relies heavily on cheap organic materials such as livestock and poultry manure and kitchen waste. Such feeds are nutritionally unbalanced and prone to breeding pathogens and parasite eggs. They may also contain residual veterinary drugs and heavy metals, posing serious safety risks to grain worm raw materials and failing to meet the quality requirements of high-end health products. Second, the hard surface of grain worms tightly encapsulates their active ingredients, making it difficult for the traditional method of directly soaking them in alcohol to fully extract the nutrients and medicinal components, resulting in low absorption efficiency and significantly reduced health benefits. Third, existing health wines containing animal-derived ingredients often use simple soaking processes, which neither address the inherent defects of the raw materials nor achieve the synergistic effect of animal protein and plant extracts. Therefore, developing a safe and reliable method for raising grain insects and a process for preparing health wine based on this raw material is of great practical significance and market value for promoting the upgrading of the insect-derived health food industry and meeting consumers' demand for high-quality health products. Summary of the Invention

[0003] In view of this, the present invention provides a health wine made from grain insects raised on fermented fresh fish feed and its preparation method. By innovating the breeding model, the safety and nutritional value of the grain insect raw material are improved from the source. Combined with a scientific formulation, the synergistic effect of animal active ingredients and plant nourishing essences is achieved, solving the problems of high safety risks and low utilization rate of effective ingredients in traditional grain insect health wines. This provides a health wine that is rich in nutrients, easily absorbed, has significant health benefits, and a mellow taste. The technical solution provided by the present invention is as follows: Firstly, this application provides a health wine made from grain insects raised on fermented fresh fish feed, composed of the following raw materials in parts by weight: 40-60 parts base liquor, 5-15 parts grain insect extract, 10-25 parts traditional Chinese medicine composition, and 0.5-2 parts excipients; the raw material for the grain insect extract is grain insects raised on fermented fresh fish feed. Furthermore, the preparation method of the fresh fish fermented feed includes: removing the scales and viscera from the fresh fish, washing it, and cutting it into pieces to obtain fish feed; mixing the fish feed, compound microbial agent, and water, and fermenting it in a sealed container to obtain fresh fish fermented feed. Furthermore, the compound microbial agent is composed of lactic acid bacteria, yeast, and Bacillus in a mass ratio of 4:3:3; the mass ratio of the fish feed, compound microbial agent, and water is 1:(0.01-0.05):(0.2-0.8). Furthermore, the fermentation temperature is 25-35℃, and the fermentation time is 5-15 days. Furthermore, the preparation method of the grain insect extract includes: raising grain insects using the fresh fish fermented feed to obtain live grain insects; washing, sterilizing, and freeze-drying the live grain insects into powder; then reflux extraction with base liquor; filtering and concentrating to obtain the grain insect extract; the fresh fish fermented feed is prepared by any of the above-described methods for preparing fresh fish fermented feed. Furthermore, the base liquor is a baijiu (Chinese white liquor) with an alcohol content of 50-60 degrees. Furthermore, the traditional Chinese medicine composition consists of the following raw materials in parts by weight: 5-10 parts of Polygonatum sibiricum, 3-8 parts of Lycium barbarum, 3-8 parts of Morus alba, 2-5 parts of Cistanche deserticola, and 2-5 parts of Morinda officinalis. Furthermore, the excipient is rock sugar. Secondly, this application also provides a method for preparing a health wine based on *Pteris vittata* (a type of insect) raised on fermented fresh fish feed, comprising the following steps: S1. Preparing the traditional Chinese medicine extract: Wash and slice the raw materials in the traditional Chinese medicine composition, extract by soaking and decocting, and concentrate to obtain the traditional Chinese medicine extract; S2. Mixing and aging: Mix the base liquor, *Pteris vittata* extract, traditional Chinese medicine extract, and excipients in proportion, stir evenly, and seal for aging for 30-90 days; S3. Finished product processing: After aging, filter through a filter membrane and aseptically fill to obtain the finished health wine.The beneficial effects of this invention are as follows: 1. This invention uses fresh fish as the fermentation substrate. Standardized pre-treatment processes, including scaling, evisceration, washing, and cutting, prevent contamination from the source. Fermentation with compound microbial agents breaks down the large-molecule proteins and fats in the fresh fish into easily absorbed small peptides, free amino acids, and other nutrients, while also producing probiotic metabolites. The resulting grain worms raised on this feed have a clean growth environment, free from pathogens, antibiotic residues, and heavy metal contamination, and are characterized by high protein and low fat content. The quality and safety of the raw materials far exceed those of traditional farming methods. 2. This invention abandons the traditional crude process of direct soaking and instead employs a refined process of sterilization, freeze-drying, and base liquor reflux extraction of the grain worms. Freeze-drying technology maximizes the retention of heat-sensitive nutrients, while base liquor reflux extraction utilizes the solvent and osmotic effect of ethanol to efficiently extract the core components of the grain worms, such as highly hydrophobic and difficult-to-dissolve active proteins and chitin derivatives, and convert them into small molecules, significantly improving the bioavailability and human absorption rate of the effective components. 3. This invention selects Chinese medicinal herbs such as Polygonatum sibiricum, Lycium barbarum, Morus alba, Cistanche deserticola, and Morinda officinalis, which have the effects of nourishing the liver and kidneys and replenishing essence and marrow, and then combines them with five-grain insects. Animal protein can provide a carrier for the absorption of plant polysaccharides, saponins and other components and promote their transformation, while plant essence can neutralize the cold nature of five-grain insects and enhance the warming and nourishing effect. The two work together to achieve the effects of strengthening the body, consolidating the foundation, strengthening muscles and bones and enhancing immunity. To make the objectives, technical solutions, and advantages of the present invention clearer, the technical solutions of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention. Experimental methods in the following embodiments that do not specify specific conditions are generally performed under conventional conditions or according to the conditions recommended by the manufacturer. Unless otherwise stated, percentages and parts are weight percentages and weight parts. Unless otherwise indicated, the technical and scientific terms used herein have the same meaning as commonly understood by those skilled in the art to which this application pertains. It should be noted that the terminology used herein is only for describing specific embodiments and is not intended to limit the exemplary embodiments of this application. The enzyme activity of the lactic acid bacteria in the present invention is 100,000 U / g, the enzyme activity of the yeast is 60,000 U / g, and the enzyme activity of the Bacillus is 30,000 U / g, all of which were purchased from Beijing Solarbio Science & Technology Co., Ltd. Example 1: A health tonic based on *Pteris vittata* raised on fermented fresh fish feed, comprising the following ingredients by weight: 50 parts base liquor, 10 parts *Pteris vittata* extract, 17 parts traditional Chinese medicine composition, and 1 part excipient; the *Pteris vittata* extract is made from *Pteris vittata* raised on fermented fresh fish feed. The base liquor is a 55% ABV pure sorghum liquor. The traditional Chinese medicine composition comprises the following ingredients by weight: 8 parts *Polygonatum sibiricum*, 6 parts *Lycium barbarum*, 6 parts *Morus alba*, 4 parts *Cistanche deserticola*, and 3 parts *Morinda officinalis*. Preparation of Fermented Fresh Fish Feed: Take fresh grass carp, remove scales and viscera, wash and cut into pieces; mix the fish pieces with compound microbial inoculant (composed of lactic acid bacteria, yeast and Bacillus in a mass ratio of 4:3:3) and water in a mass ratio of 1:0.04:0.5 in a fermentation tank, stir evenly, and ferment in a sealed container at 30℃ for 7 days. When the material becomes pasty, emits a strong fermented aroma, and the pH value drops to 4.0-4.5, the fermented fresh fish feed is obtained. Preparation of Grain Worm Extract: Use the above-mentioned fermented fresh fish feed to raise grain worms, collect mature larvae, wash with purified water, then soak in 75% alcohol for surface sterilization, then freeze-dry for 48 hours, and then pulverize and pass through an 80-mesh sieve to obtain grain worm dry powder. Add 5 times the amount of base wine to the grain worm dry powder, reflux extract twice at 60℃, 1.5 hours each time, combine the extracts, filter, and then concentrate under reduced pressure to 1 / 8 of the original volume to obtain grain worm extract.A method for preparing a health wine based on fermented fish feed and raised on grain insects includes the following steps: S1. Preparation of traditional Chinese medicine extract: Wash and slice Polygonatum sibiricum, Lycium barbarum, Morus alba, Cistanche deserticola, and Morinda officinalis. Soak them in 6 times the amount of base wine for 2 hours, then add 4 times the amount of water and decoct twice, the first time for 1 hour and the second time for 45 minutes. Combine the decoctions, filter, and concentrate the filtrate under reduced pressure to 1 / 8 of the original volume to obtain the traditional Chinese medicine extract; S2. Mixing and aging: Mix the base wine, grain insect extract, traditional Chinese medicine extract, and rock sugar in proportion, stir evenly, transfer to a ceramic jar, seal and place in a cool, dark place to age for 60 days, stirring once every 10 days during this period; S3. Finished product processing: After aging, filter first through a 0.45μm microporous membrane, then perform aseptic filtration, fill into pre-sterilized brown glass bottles, seal, and label to obtain the health wine. Example 2: A health tonic based on *Pteris vittata* raised on fermented fresh fish feed, comprising the following ingredients by weight: 40 parts base liquor, 5 parts *Pteris vittata* extract, 10 parts traditional Chinese medicine composition, and 0.5 parts excipients; the *Pteris vittata* extract is made from *Pteris vittata* raised on fermented fresh fish feed. The base liquor is a 50% ABV pure sorghum liquor. The traditional Chinese medicine composition comprises the following ingredients by weight: 5 parts *Polygonatum sibiricum*, 3 parts *Lycium barbarum*, 3 parts *Morus alba*, 2 parts *Cistanche deserticola*, and 2 parts *Morinda officinalis*. Preparation of Fermented Fresh Fish Feed: Take fresh grass carp, remove scales and viscera, wash and cut into pieces; mix the fish pieces with compound microbial inoculant (composed of lactic acid bacteria, yeast and Bacillus in a mass ratio of 4:3:3) and water in a mass ratio of 1:0.05:0.8 in a fermentation tank, stir evenly, and ferment in a sealed container at 35℃ for 10 days. When the material becomes pasty, emits a strong fermented aroma, and the pH value drops to 4.0-4.5, the fermented fresh fish feed is obtained. Preparation of Grain Worm Extract: Use the above-mentioned fermented fresh fish feed to raise grain worms, collect mature larvae, wash with purified water, then soak in 75% alcohol for surface sterilization, then freeze-dry for 48 hours, and then pulverize and pass through an 80-mesh sieve to obtain grain worm dry powder. Add 8 times the amount of base wine to the grain worm dry powder, reflux extract twice at 60℃, 1.5 hours each time, combine the extracts, filter, and then concentrate under reduced pressure to 1 / 8 of the original volume to obtain grain worm extract. A method for preparing a health wine based on fermented fish feed and raised on grain insects includes the following steps: S1. Preparation of traditional Chinese medicine extract: Wash and slice Polygonatum sibiricum, Lycium barbarum, Morus alba, Cistanche deserticola, and Morinda officinalis. Soak them in 5 times the amount of base wine for 2 hours, then add 3 times the amount of water and decoct twice, the first time for 1 hour and the second time for 45 minutes. Combine the decoctions, filter, and concentrate the filtrate under reduced pressure to 1 / 8 of the original volume to obtain the traditional Chinese medicine extract; S2. Mixing and aging: Mix the base wine, grain insect extract, traditional Chinese medicine extract, and rock sugar in proportion, stir evenly, transfer to a ceramic jar, seal and place in a cool, dark place to age for 90 days, stirring once every 10 days during the period; S3. Finished product processing: After aging, filter first through a 0.45μm microporous membrane, then perform aseptic filtration, fill into pre-sterilized brown glass bottles, seal, and label to obtain the health wine.A comparative example of a health tonic based on *Polygonum multiflorum* (a type of insect) is provided, comprising the following ingredients by weight: 50 parts base liquor, 10 parts *Polygonum multiflorum* extract, 17 parts traditional Chinese medicine composition, and 1 part excipients. The *Polygonum multiflorum* extract is derived from *Polygonum multiflorum* larvae raised using kitchen waste. The base liquor is a 55% ABV pure sorghum liquor. The traditional Chinese medicine composition comprises the following ingredients by weight: 8 parts *Polygonatum sibiricum*, 6 parts *Lycium barbarum*, 6 parts *Mulberry*, 4 parts *Cistanche deserticola*, and 3 parts *Morinda officinalis*. Preparation of the *Polygonum multiflorum* extract: *Polygonum multiflorum* larvae are raised using kitchen waste. Mature larvae are collected, washed with purified water, and then surface-sterilized by soaking in 75% alcohol. They are then freeze-dried for 48 hours, pulverized, and passed through an 80-mesh sieve to obtain *Polygonum multiflorum* powder. Five times the amount of base liquor is added to the powder, and the mixture is refluxed twice at 60°C for 1.5 hours each time. The extracts are combined, filtered, and then concentrated under reduced pressure to 1 / 8 of the original volume to obtain the *Polygonum multiflorum* extract. A method for preparing a health tonic based on *Pteris vittata* (a type of insect) is consistent with Example 1. Compared to Example 1, the raw material for this comparative example, the *Pteris vittata* extract, is *Pteris vittata* raised using kitchen waste. Experimental Example: Determination of protein content in *Pteris vittata* extract: 30 μL of 5 mg / mL BSA standard solution was added to 120 μL of PBS solution to prepare a BSA protein standard with a final concentration of 1 mg / mL. The 1 mg / mL standard was diluted to 0.75, 0.5, 0.25, 0.125, 0.025, and 0 mg / mL. An appropriate amount of *Pteris vittata* extract was weighed and dissolved in double-distilled water to obtain a *Pteris vittata* extract sample with a concentration of 1 mg / mL. A BCA working solution was prepared by adding 20 μL of Cu reagent to 1 mL of BCA reagent. Following the instructions of the kit (BCA protein concentration assay kit, purchased from Beijing Solarbio Science & Technology Co., Ltd.), 2 μL of BSA protein standards of various concentrations and *Ceratophyllum demersum* extract samples were added to a 96-well plate. The plate was diluted to 20 μL with PBS solution, and then 200 μL of BCA working solution was added. The plate was incubated at 37°C for 30 min in the dark. The plate was then removed and placed in a microplate reader to measure the absorbance at 562 nm. A standard curve was plotted with the concentration of each BSA standard on the x-axis and the absorbance of each sample at 562 nm on the y-axis. The protein content of each sample was calculated, and the obtained concentration was the concentration obtained by diluting each sample 10 times. Table 1 shows the protein content in *Ceratophyllum demersum* extract at a concentration of 1 mg / mL. (Table data follows, but is not translated as it is not part of the instructions.) As shown in Table 1, the protein content of the *Phyllostachys edulis* extract prepared from *Phyllostachys edulis* raised using fermented fresh fish feed is significantly higher than that of the extract raised using traditional kitchen waste. This demonstrates that the fermented fresh fish feed method of this invention can effectively enhance the protein accumulation of *Phyllostachys edulis* and significantly optimize the nutritional value of the raw materials. The present invention and its embodiments have been described above. This description is not restrictive, and the embodiments shown are only one of the embodiments of the present invention; the actual structure is not limited to this. In conclusion, if those skilled in the art are inspired by this invention and design similar structures and embodiments without departing from the spirit of the invention, they should all fall within the protection scope of this invention.

Claims

1. A health wine made from grain insects raised on fermented fresh fish feed, characterized in that, It is composed of the following raw materials in parts by weight: 40-60 parts base liquor, 5-15 parts five-grain insect extract, 10-25 parts traditional Chinese medicine composition, and 0.5-2 parts excipients; the raw material of the five-grain insect extract is five-grain insects raised using fresh fish fermented feed.

2. The health wine based on grain insects raised on fermented fresh fish feed according to claim 1, characterized in that, The preparation method of the fresh fish fermented feed includes: removing the scales and viscera of the fresh fish, washing it, and cutting it into pieces to obtain fish feed; mixing the fish feed, compound microbial agent and water, and fermenting it in a sealed container to obtain fresh fish fermented feed.

3. A health wine based on grain insects raised on fermented fresh fish feed according to claim 2, characterized in that, The compound microbial agent is composed of lactic acid bacteria, yeast and Bacillus in a mass ratio of 4:3:3; the mass ratio of fish feed, compound microbial agent and water is 1:(0.01-0.05):(0.2-0.8).

4. A health wine based on grain insects raised on fermented fresh fish feed according to claim 2, characterized in that, The fermentation temperature is 25-35℃, and the fermentation time is 5-15 days.

5. A health wine based on grain insects raised on fermented fresh fish feed according to claim 1, characterized in that, The preparation method of the *Phyllostachys edulis* extract includes: raising *Phyllostachys edulis* using fresh fish fermented feed to obtain live *Phyllostachys edulis*; cleaning, sterilizing, freeze-drying, pulverizing and sieving the live *Phyllostachys edulis* to obtain dried *Phyllostachys edulis* powder; reflux extraction of the dried *Phyllostachys edulis* powder with base liquor; and filtration and concentration to obtain the *Phyllostachys edulis* extract; wherein the fresh fish fermented feed is prepared by the method of fresh fish fermented feed preparation according to any one of claims 2-4.

6. A health wine based on grain insects raised on fermented fresh fish feed according to claim 1, characterized in that, The medicinal herb composition consists of the following raw materials in parts by weight: 5-10 parts of Polygonatum sibiricum, 3-8 parts of Lycium barbarum, 3-8 parts of Morus alba, 2-5 parts of Cistanche deserticola, and 2-5 parts of Morinda officinalis.

7. A health wine based on grain insects raised on fermented fresh fish feed according to claim 1, characterized in that, The auxiliary ingredient is rock sugar.

8. A health wine based on grain insects raised on fermented fresh fish feed according to claim 1, characterized in that, The base liquor is a type of baijiu with an alcohol content of 50-60%.

9. A method for preparing a health wine based on grain insects raised on fermented fresh fish feed, as described in claims 1-8, characterized in that... Includes the following steps: S1. Preparation of Traditional Chinese Medicine Extract: Wash and slice the raw materials in the traditional Chinese medicine composition, extract them by soaking and decocting, and concentrate them to obtain the traditional Chinese medicine extract; S2. Mixing and Aging: Mix the base wine, the extract of five grains insects, the traditional Chinese medicine extract and excipients in proportion, stir evenly, and seal and age for 30-90 days; S3. Finished Product Processing: After aging, filter through a filter membrane and aseptically fill to obtain the health wine.