Bioreactor with controllable observation function and used for culturing biological samples

Abstract

The invention provides a bioreactor having a controllable observation function and used for culturing biological samples, the bioreactor comprises a shell, the shell is provided with a first chamber, a second chamber and a control module, and the first chamber and the second chamber are both provided with cabin doors; the first chamber is internally provided with a motion platform used for bearing a cell culture plate or a micro-fluidic chip; the environment control module is used for regulating and controlling internal environment parameters of the first chamber; the imaging module is used for observing a biological sample in the bearing cell culture plate or the micro-fluidic chip; an injection assembly is arranged in the second chamber and is used for conveying or extracting liquid required by the cell culture plate or the micro-fluidic chip; the control module is in signal connection with the motion platform, the environment control module, the imaging module and the injection assembly. Seamless integration of culture, perfusion, stimulation and in-situ observation is achieved, the device can be adapted to various culture carriers, flow velocity accurate adjustment and long-time unattended monitoring are supported, the pollution risk is remarkably reduced, and experiment repeatability and efficiency are improved.

Description

A bioreactor with controllable observation capabilities for culturing biological samples. Technical Field

[0001] This invention relates to the field of cell culture and bioreactor technology, and more specifically, to a bioreactor with controllable observation capabilities for culturing biological samples. Background Technology

[0002] Cell culture and dynamic observation are key methods in modern life science research, drug development, and toxicological evaluation. Traditional cell culture typically uses static culture dishes or flasks, requiring manual replacement of the culture medium and periodic sample removal for microscopic observation. This method is not only cumbersome and prone to contamination, but also fails to achieve continuous, non-destructive, in-situ monitoring of cell growth, making it difficult to capture the real-time dynamic response of cells to external stimuli (such as drugs, metabolites, and fluid shear forces).

[0003] To improve culture and observation conditions, existing technologies include cell culture incubators or incubation systems with environmental control (such as temperature and CO2), as well as microscope incubation devices for live cell imaging. Furthermore, the combination of microfluidics and bioreactors makes cell culture and stimulation possible in a controlled fluid environment. However, these systems typically have the following limitations: fragmented functional modules: culture control, fluid delivery, and microscopic observation are often performed by multiple independent devices (such as incubators, peristaltic pumps, and inverted microscopes). Frequent sample movement during experiments not only disrupts environmental stability but also increases the risk of contamination and operational complexity.

[0004] Low automation and integration: Most systems lack a unified software control platform. Operations such as flow rate adjustment, sample switching, and multi-point timed imaging require manual intervention, making it difficult to achieve long-term, fully automated experimental procedures. The repeatability and throughput of experiments are limited.

[0005] Insufficient flexibility: Existing devices are usually designed for specific culture carriers (such as multi-well plates of a certain size or specific chips), making it difficult to be compatible with various culture and detection platforms, from standard multi-well plates to custom microfluidic chips, thus limiting their application scope.

[0006] The precision and uniformity of environmental control need to be improved: For some demanding experiments that require precise control of the local microenvironment (such as precise timing of drug administration, temperature steps) or long-term maintenance of sterility, the stability and reliability of existing equipment still face challenges.

[0007] Therefore, there is an urgent need to develop a highly integrated, automated, flexible, and precisely environmentally controlled bioreactor system that can seamlessly integrate culture, perfusion, stimulation, and in-situ dynamic observation, providing a more powerful and reliable research tool for fields such as cell biology, tissue engineering, and drug screening. Summary of the Invention

[0008] The purpose of this invention is to provide a bioreactor with controllable observation function for culturing biological samples, which addresses the shortcomings of existing technologies and solves the problems mentioned in the background.

[0009] To solve the above-mentioned technical problems, the technical solution adopted by the present invention is as follows: a bioreactor with controllable observation function for culturing biological samples, comprising a shell, the shell having a first chamber, a second chamber, and a control module, both the first and second chambers being provided with hatches; the first chamber being provided with: a motion platform for carrying cell culture plates or microfluidic chips; an environmental control module for regulating the internal environmental parameters of the first chamber; and an imaging module for observing the biological samples in the cell culture plates or microfluidic chips; the second chamber being provided with an injection assembly for delivering or extracting the liquid required by the cell culture plates or microfluidic chips; the control module being signal-connected to the motion platform, the environmental control module, the imaging module, and the injection assembly.

[0010] Furthermore, the motion platform is an XY two-axis electric platform.

[0011] Furthermore, the environmental control module includes: a temperature control unit for regulating the temperature inside the first chamber; a CO2 concentration control unit for regulating the CO2 concentration inside the first chamber; and a sterilization unit for sterilizing the first chamber.

[0012] Furthermore, the temperature control unit includes a temperature sensor and a heating unit, and the temperature control unit achieves real-time monitoring and adjustment through a control module.

[0013] Furthermore, the CO2 concentration control unit includes a CO2 concentration sensor and a CO2 storage unit. The housing is provided with an interface for connecting the CO2 storage unit to the first chamber. The CO2 concentration control unit realizes real-time monitoring and adjustment through a control module.

[0014] Furthermore, the sterilization unit is an ultraviolet lamp, which is connected to the control module and can be turned on or off according to experimental requirements.

[0015] Furthermore, the imaging module includes an inverted microscope and an image acquisition system. The eyepiece of the inverted microscope and the camera lens of the image acquisition system are mounted on the top of the first chamber, above the motion platform, while the objective lens of the inverted microscope is located below the motion platform.

[0016] Furthermore, the injection assembly includes a first mounting base, a slider, a fixing rod, a drive unit, a lead screw, a syringe, and a second mounting base. The first mounting base, slider, and second mounting base are sequentially connected by the fixing rod, and the slider is slidably connected to the fixing rod. The first and second mounting bases are spaced apart on the base. One end of the lead screw passes through the first mounting base, the slider, and is rotatably connected to the second mounting base. The drive unit is fixedly connected to the other end of the lead screw and mounted on the first mounting base. The lead screw is threadedly connected to the slider. The slider is provided with a first fixing member for fixing the syringe piston handle, and the second mounting base is provided with a second fixing member for fixing the syringe empty cylinder. The syringe tip is connected to a cell culture plate or microfluidic chip via tubing.

[0017] Furthermore, the control module includes a human-machine interface, which is located outside the housing and is used for setting experimental parameters, monitoring equipment status, and acquiring data.

[0018] This invention offers at least the following advantages or beneficial effects: The bioreactor with controllable observation capabilities for culturing biological samples provides researchers the ability to flexibly adjust flow rates, culture plate types, and observation parameters according to different experimental needs, thereby achieving efficient culture and observation of various biological samples. The coordination between the peristaltic pump and the motion platform in the second chamber enhances the applicability of the equipment. Through external computer control, researchers can precisely adjust the flow rate of each injection component, ensuring accurate delivery of culture medium or nutrient solution. This feature improves experimental reproducibility and helps obtain more reliable experimental results. The built-in microscope and image acquisition system allow researchers to conduct continuous photography and cell growth monitoring for extended periods without opening the bioreactor door, effectively reducing the risk of contamination during the experiment. The efficient lighting system and controllable ultraviolet light equipped in this invention ensure sterile experimental conditions, further reducing the risk of external contamination. Furthermore, real-time monitoring and adjustment of temperature and CO2 concentration provide a stable growth environment for cells. The user-friendly interface design allows users with different backgrounds to easily set experimental parameters, monitor status, and acquire data, thereby improving experimental efficiency and convenience. This invention not only supports the culture of cells, microspheres, and tumor spheres, but is also compatible with various experimental devices such as microfluidic chips. Its highly adaptable motion platform meets the needs of different experiments. The equipment is designed to protect the experimental environment, reduce potential contamination risks during operation, and effectively ensure the safety of laboratory operations. Attached Figure Description

[0019] To more clearly illustrate the technical solutions of the embodiments of the present invention, the accompanying drawings used in the embodiments will be briefly introduced below. It should be understood that the following drawings only show some embodiments of the present invention and should not be regarded as a limitation on the scope. For those skilled in the art, other related drawings can be obtained based on these drawings without creative effort.

[0020] Figure 1 is a schematic diagram of the structure of a bioreactor with controllable observation function for culturing biological samples provided by the present invention; Figure 2 is a schematic diagram of the internal structure of the first chamber provided by the present invention; Figure 3 is a schematic diagram of the internal structure of the second chamber provided by the present invention; Figure 4 is a schematic diagram of the structure of the injection assembly provided by the present invention; Figure 5 is a three-dimensional schematic diagram of the motion platform provided by the present invention; Figure 6 is a top view of the motion platform provided by the present invention; Figure 7 is a cross-sectional view at AA in Figure 6; Figure 8 is a cross-sectional view at CC in Figure 6; Figure 9 is an enlarged view of the structure at A in Figure 7.

[0021] Icons: 1. Shell; 2. First chamber; 3. Second chamber; 4. Door; 5. Motion platform; 50. Fixing component; 51. Base plate; 52. X-axis moving plate; 53. Y-axis moving plate; 54. Slide rail assembly; 55. First drive device; 56. Threaded rod; 57. Threaded hole; 58. Second drive device; 6. Eyepiece; 7. Objective lens; 8. Injection assembly; 80. Base; 81. First mounting base; 82. Slider; 83. Fixing rod; 84. Drive unit; 85. Lead screw; 87. Second mounting base; 88. First fixing component; 89. Second fixing component. Detailed Implementation

[0022] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. The components of the embodiments of the present invention described and shown in the accompanying drawings can generally be arranged and designed in various different configurations.

[0023] Please refer to Figures 1 to 3. A bioreactor with controllable observation function for culturing biological samples includes a shell 1. The shell 1 is provided with a first chamber 2, a second chamber 3 and a control module. Both the first chamber 2 and the second chamber 3 are provided with hatches 4 for opening and closing the first chamber 2 and the second chamber 3.

[0024] The first chamber 2 is equipped with: a motion platform 5 for carrying cell culture plates or microfluidic chips, an environmental control module for regulating the internal environmental parameters of the first chamber 2, and an imaging module for observing biological samples in the cell culture plates or microfluidic chips.

[0025] Specifically, the imaging module includes an inverted microscope and an image acquisition system. The inverted microscope is a Nikon TS2, equipped with a dedicated camera port, supporting simultaneous camera connection for image acquisition and real-time observation. The eyepiece 6 of the inverted microscope and the camera lens of the image acquisition system are mounted on top of the first chamber 2, above the motion platform 5, while the objective lens 7 of the inverted microscope is located below the motion platform 5. Both the inverted microscope and the image acquisition system are programmed and controlled via a control module, enabling automatic focusing and imaging at multiple designated locations on the motion platform at preset time intervals.

[0026] The imaging module allows for observation without opening the bioreactor's hatch, avoiding environmental fluctuations and contamination risks associated with such opening. Secondly, the preset time interval imaging function enables long-term, continuous observation, capturing sample changes at different points in time to generate comprehensive dynamic monitoring data. Furthermore, the ability to capture images at multiple designated locations allows researchers to simultaneously monitor samples from multiple regions on the culture medium, improving observation efficiency. The acquired image data can be transmitted in real-time to an external computer via the control module for subsequent image analysis, such as cell morphology change analysis and cell proliferation counting.

[0027] Please refer to Figures 5 to 9. The motion platform 5 is a two-axis motorized platform (X and Y axes) mounted on an inverted microscope. It can adapt to and precisely position various sizes of cell culture plates and microfluidic chips, including but not limited to 24-well, 48-well, and 96-well standard cell culture plates, as well as custom-made microfluidic chips of different sizes. The motion platform 5 includes a base plate 51, an X-axis moving plate 52, a Y-axis moving plate 53, and multiple sets of slide rails 54. A through-type clearance window is provided in the middle of the base plate 51, the X-axis moving plate 52, and the Y-axis moving plate 53 for the objective lens 7 to observe the cell culture plate or microfluidic chip located on the Y-axis moving plate 53. The base plate 51 is fixedly connected to the microscope via fasteners 50 arranged around its periphery. Two sets of slide rails 54 are spaced apart on the base plate 51, arranged along its length. Each slide rail set includes a fixed slide rail and a movable slide rail arranged in parallel. Limiting grooves are formed on opposite sides of the fixed and movable slide rails, and limiting members are provided between them. The two sides of the limiting members are located within the limiting grooves of the fixed and movable slide rails, respectively, thereby allowing the movable slide rail to move along the length of the limiting members. The X-axis moving plate 52 is slidably connected to the base plate 51 via these two sets of slide rails 54. The X-axis moving plate 52 is connected to the movable slide rail by screws, and the fixed slide rail is connected to the base plate 51 by screws. The two sets of slide rails 54 mutually limit each other, preventing the movable slide rail within the same set from separating from the limiting members. A first drive device 55, which is a stepper motor, is installed in the middle of the base plate 51. A threaded rod 56 is coaxially mounted on its output shaft, and the threaded rod 56 is parallel to the fixed slide rail. A threaded hole 57 that matches the threaded rod 56 is provided on the X-axis moving plate 52. By controlling the forward and reverse rotation of the first drive device 55, the X-axis moving plate 52 can be driven to reciprocate in the X-axis direction.

[0028] In addition, two sets of slide rails 54 are spaced apart on the X-axis moving plate 52, and these two sets of slide rails 54 are arranged along the Y-axis direction. The X-axis moving plate 52 is connected to the fixed slide rails in the two sets of slide rails 54 by screws, and the Y-axis moving plate 53 is connected to the movable slide rails in the two sets of slide rails 54 by screws, thereby realizing the sliding connection between the X-axis moving plate 52 and the Y-axis moving plate 53. A second drive device 58 is provided at one end of the X-axis moving plate 52. The second drive device 58 is also a stepper motor, and a threaded rod 56 is coaxially provided on its output shaft. The threaded rod 56 and the fixed slide rails on the X-axis moving plate 52 are arranged parallel to each other. The Y-axis moving plate 53 is also provided with threaded holes 57 that are adapted to the threaded rod 56. By controlling the forward and reverse rotation of the second drive device 58, the Y-axis moving plate 53 can be driven to reciprocate in the Y-axis direction.

[0029] The XY-axis movement capability of motion platform 5 allows any position on the culture medium to be precisely positioned within the observation field of the imaging module, ensuring comprehensiveness and accuracy of observation. This flexible and adaptable design allows researchers to select appropriate culture media according to specific experimental needs without the need for additional equipment adapters, greatly enhancing the applicability of the equipment.

[0030] The environmental control module includes: a temperature control unit for regulating the temperature inside the first chamber 2, a CO2 concentration control unit for regulating the CO2 concentration inside the first chamber 2, and a sterilization unit for sterilizing the first chamber 2.

[0031] The temperature control unit includes a temperature sensor and a heating unit, which are monitored and adjusted in real time via a control module. The heating unit consists of an existing heater and cooling jacket. Both the temperature sensor and the heating unit are electrically connected to the control module. The operator can set the temperature of the first chamber 2 via the control module. The temperature sensor detects the temperature inside the first chamber 2 in real time and transmits the signal to the control module. The control module compares the real-time temperature value inside the first chamber 2 with the preset temperature value and controls the heating unit to operate, cooling or heating the first chamber 2 to maintain the temperature within the experimentally set value, providing a suitable growth temperature environment for biological samples, such as the 37°C commonly used for mammalian cell culture. By monitoring the temperature inside the chamber in real time through the temperature sensor and feeding the temperature data back to the control module, the control module adjusts the temperature according to the preset value to ensure that the temperature remains stable within the set range, avoiding adverse effects of temperature fluctuations on sample growth.

[0032] The CO2 concentration control unit includes a CO2 concentration sensor and a CO2 storage unit. The housing 1 has an interface for connecting the CO2 storage unit to the first chamber 2. The CO2 concentration control unit monitors and adjusts the CO2 concentration in real time via a control module to maintain a constant CO2 concentration within the first chamber 2. Stable CO2 concentration is crucial for maintaining the pH of the cell culture medium and is a key condition for normal cell growth. The CO2 storage unit is a CO2 storage tank connected to the interface via a pipe equipped with a solenoid valve. Both the CO2 concentration sensor and the solenoid valve are electrically connected to the control module. The CO2 concentration sensor detects the CO2 concentration within the chamber in real time and transmits the data to the control module. The control module adjusts the CO2 flow rate according to a preset concentration value to ensure the CO2 concentration is maintained at a set level, such as the 5% CO2 concentration required for conventional cell culture.

[0033] The sterilization unit is an existing ultraviolet lamp connected to the control module. Preferably, a sterilization unit is also installed in the second chamber 3. This unit is used to sterilize the first chamber 2 and / or the second chamber 3 to ensure a sterile experimental environment and reduce the risk of sample contamination. Researchers can select to sterilize the first chamber 2 alone, the second chamber 3 alone, or both chambers simultaneously, according to experimental needs, via the control module. The ultraviolet lamp's sterilization function effectively kills bacteria, fungi, and other microorganisms within the chambers, providing a clean environment for biological sample culture and reducing experimental failures caused by environmental microbial contamination.

[0034] Referring to Figures 3 and 4, at least one injection assembly 8 is installed in the second chamber 3. The injection assembly 8 is electrically connected to the control module and is used for the delivery or extraction of liquids required for cell culture plates or microfluidic chips. The injection assembly 8 supports bidirectional fluid delivery functions of pushing and aspirating, making it possible to recycle culture medium, wash samples, and other operations, further expanding the flexibility of experiments.

[0035] Specifically, the injection assembly 8 includes a base 80, a first mounting base 81, a slider 82, a fixing rod 83, a drive unit 84, a lead screw 85, a syringe, and a second mounting base 87. The first mounting base 81, the slider 82, and the second mounting base 87 are sequentially connected by the fixing rod 83, and the slider 82 is slidably connected to the fixing rod 83. The first mounting base 81 and the second mounting base 87 are fixedly mounted on the cavity wall of the second chamber 3 via the base 80, and there is a gap between the slider 82 and the base 80 to facilitate the movement of the slider 82.

[0036] One end of the lead screw 85 passes through the first mounting base 81 and the slider 82 in sequence, and extends into the second mounting base 87, where it is rotatably connected. The lead screw 85 and the slider 82 are threadedly connected. The drive unit 84 is a stepper motor, which is fixedly connected to the other end of the lead screw 85 and mounted on the first mounting base 81. When the drive unit 84 is running, it drives the lead screw 85 to rotate. Since the slider 82 is restricted by the fixed rod 83 and cannot rotate with the lead screw 85, it is converted into linear movement along the fixed rod 83.

[0037] A first fixing member 88 for securing the syringe piston handle is provided on the slider 82. The first fixing member 88 is a clip, which is detachably provided on the side of the slider 82 near the second mounting base 87 by existing snap-fit ​​buckles or screws. A gap is provided between the clip and the slider 82 to engage the syringe piston handle. Preferably, a notch is provided on the clip to allow the syringe piston rod to pass.

[0038] A second fixing member 89 for fixing the syringe empty cylinder is provided on the second mounting base 88. The second fixing member 89 is a pressure plate, which is installed on the top of the second mounting base 88 by screws. The pressure plate and the second mounting base 88 compress and fix the syringe empty cylinder, thereby fixing it. Preferably, the top of the second mounting base 88 has a V-shaped groove for placing the syringe empty cylinder.

[0039] The syringe tip is connected to the cell culture plate or microfluidic chip after passing through the partition between the first chamber 2 and the second chamber 3 via a tubing.

[0040] After securing the syringe using the first fixing component 88 and the second fixing component 89, the operator connects the nipple to the end of the tubing, ensuring a leak-proof seal. The required fluid delivery is then input into the control module, which then controls the injection assembly to deliver or extract liquid at specific times and quantities.

[0041] Preferably, the control module includes a human-machine interface (HMI) located externally to the housing, used for setting experimental parameters, monitoring equipment status, and acquiring data. Furthermore, the control module supports remote control via an external computer. Researchers can install corresponding control software on their computers to set experimental parameters, monitor the experimental process, and acquire experimental data. Remote control further enhances the convenience of the experiment, allowing researchers to start and monitor experiments without being physically present at the equipment. The HMI includes: a system bar containing the system name and management modules; a menu bar providing quick access to platform functions and operations; and a run operation bar controlling the experimental status during task execution, such as pausing, starting, and terminating to complete the specified task. Specific operations include: selecting an available plate slot, filling in task information, selecting an experimental method (different methods provide different information; experimental methods are maintained in "Management - Experimental Method Management"), selecting the wells for sample loading, and performing sample loading or batch filling of information for the wells. Save. Adjust and modify the hole position information in the list. Click Run to start the task.

[0042] The invention provides a method for using a multifunctional bioreactor, comprising the following steps: Step 1, close all doors and click on the screen in front of the bioreactor to turn on the ultraviolet lamp for sterilization; Step 2, after turning off the ultraviolet lamp, open the peristaltic pump door on the left side of the bioreactor and install a syringe containing culture medium or nutrient solution on the injection assembly; Step 3, open the door in front of the bioreactor and install a cell culture plate or microfluidic chip containing target cells or microbial suspension in two corresponding positions on the motion platform; Step 4, use a flow tube to correctly connect the syringe to the cell culture plate or microfluidic chip on the motion platform; Step 5, close the doors and set a constant temperature and CO2 concentration inside the chamber on the screen in front of the bioreactor; Step 6, set the target flow rate of the syringe and the total amount of liquid contained in the syringe on the computer; Step 7, select the type of cell culture plate and the well position where the sample has been added using microscope and photography software on the computer, and set parameters such as the number of photos and the photo interval, selecting as needed; Step 8, start the bioreactor to achieve automatic culture and continuous observation and photography.

[0043] The above are merely preferred embodiments of the present invention and are not intended to limit the present invention. Various modifications and variations can be made to the present invention by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the scope of protection of the present invention.

Claims

1. A bioreactor with controllable observation function for culturing biological samples, characterized in that, The device includes a housing, which comprises a first chamber, a second chamber, and a control module. Both the first and second chambers are equipped with hatches. The first chamber contains: a motion platform for carrying cell culture plates or microfluidic chips; an environmental control module for regulating the internal environmental parameters of the first chamber; and an imaging module for observing biological samples in the cell culture plates or microfluidic chips. The second chamber contains an injection assembly for delivering or extracting the required liquids from the cell culture plates or microfluidic chips. The control module is signal-connected to the motion platform, the environmental control module, the imaging module, and the injection assembly.

2. A bioreactor with controllable observation function for culturing biological samples according to claim 1, characterized in that, The motion platform is an XY two-axis electric platform.

3. A bioreactor with controllable observation function for culturing biological samples according to claim 1, characterized in that, The environmental control module includes: a temperature control unit for regulating the temperature inside the first chamber; a CO2 concentration control unit for regulating the CO2 concentration inside the first chamber; and a sterilization unit for sterilizing the first chamber.

4. A bioreactor with controllable observation function for culturing biological samples according to claim 3, characterized in that, The temperature control unit includes a temperature sensor and a heating unit, and the temperature control unit achieves real-time monitoring and adjustment through a control module.

5. A bioreactor with controllable observation function for culturing biological samples according to claim 3, characterized in that, The CO2 concentration control unit includes a CO2 concentration sensor and a CO2 storage unit. The housing is provided with an interface for connecting the CO2 storage unit to the first chamber. The CO2 concentration control unit realizes real-time monitoring and adjustment through a control module.

6. A bioreactor with controllable observation function for culturing biological samples according to claim 4, characterized in that, The sterilization unit is an ultraviolet lamp, which is connected to the control module and can be turned on or off according to experimental needs.

7. A bioreactor with controllable observation function for culturing biological samples according to claim 1, characterized in that, The imaging module includes an inverted microscope and an image acquisition system. The eyepiece of the inverted microscope and the camera lens of the image acquisition system are mounted on the top of the first chamber, above the motion platform, while the objective lens of the inverted microscope is located below the motion platform.

8. A bioreactor with controllable observation function for culturing biological samples according to claim 1, characterized in that, The injection assembly includes a base, a first mounting base, a slider, a fixing rod, a drive unit, a lead screw, a syringe, and a second mounting base. The first mounting base, slider, and second mounting base are sequentially connected by the fixing rod, and the slider is slidably connected to the fixing rod. The first and second mounting bases are spaced apart on the base. One end of the lead screw passes through the first mounting base, the slider, and is rotatably connected to the second mounting base. The drive unit is fixedly connected to the other end of the lead screw and mounted on the first mounting base. The lead screw is threadedly connected to the slider. The slider is provided with a first fixing member for fixing the syringe piston handle, and the second mounting base is provided with a second fixing member for fixing the syringe empty cylinder. The syringe tip is connected to a cell culture plate or microfluidic chip via tubing.

9. A bioreactor with controllable observation function for culturing biological samples according to claim 1, characterized in that, The control module includes a human-machine interface, which is located outside the housing and is used for setting experimental parameters, monitoring equipment status, and acquiring data.

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