Scalp repair composition containing epidermis repair polysaccharide and saccharomycetes lysate

Abstract

The invention relates to the technical field of daily chemical products and cosmetics, in particular to a scalp repair composition containing epidermis repair polysaccharide and saccharomycetes lysate. The skin repairing composition is prepared from the following raw materials in percentage by mass: 1 to 5 percent of skin repairing polysaccharide; 1%-5% of a yeast lysate; 1%-10% of PEG-5 (Polyethylene Glycol) glycerate triisostearate; and the balance of cosmetically acceptable matrix. The polysaccharide is mannan which is obtained by fermenting Paenibacillus sp.1538, and the weight-average molecular weight of the mannan is 6.15 * 10 < 6 > Da. The specific ultra-high molecular weight polysaccharide is compounded with the saccharomyces cerevisiae extract, and the low-irritation PEG-5 glycerate triisostearate is introduced, so that the compatibility problem that the high molecular weight polysaccharide is easy to flocculate is effectively solved. The composition can form a film immediately, deeply resist inflammation, relieve and repair a physical barrier of scalp, has an excellent synergistic effect, and solves the problem of scalp chemical stimulation injury caused by a traditional surfactant from the source.

Description

Technical Field

[0001] This invention relates to the field of daily chemical products and cosmetics technology, and more specifically, to a scalp repair composition comprising epidermal repair polysaccharides and yeast lysate. Background Technology

[0002] With the fast pace of life and increasing environmental pressure, scalp microecological imbalances, such as dandruff, abnormal sebum production, scalp itching, erythema, and papules, are becoming increasingly serious. Modern hair care products inevitably contain various surfactants (cationic, anionic, amphoteric, or nonionic), antibacterial agents, or hair dyes. While these chemicals cleanse the scalp, they often thin the stratum corneum or reduce its water saturation, stimulating scalp cells to produce inflammatory factors, leading to cell apoptosis and scaling—in other words, chemical irritation and damage.

[0003] Currently, to improve the aforementioned scalp problems, most commercially available scalp care products use single active ingredients (such as panthenol and ceramides) for basic moisturizing, or use chemical anti-dandruff ingredients (such as zinc pyrithione ZPT) to inhibit Malassezia. However, these methods have drawbacks such as high irritation and a tendency to induce drug resistance. In recent years, the industry has begun to explore the use of bio-fermentation technology and polysaccharides to regulate the scalp microecology. For example, Chinese patent document CN118453491B discloses a scalp care liquid containing probiotic fermentation products, which mainly uses fermentation filtrate such as Bifida Ferment Lysate for basic repair; another example is Chinese patent document CN114681362A, which discloses a shampoo that improves the scalp microecology by combining common polysaccharides with surfactants such as amino acids. In addition, preliminary disclosures have been made in the industrial and agricultural fields regarding the fermentation and polysaccharide extraction of Paenibacillus (such as CN109666603B).

[0004] However, existing technologies still have the following significant limitations when dealing with complex chemical irritation damage to the scalp: First, the conventional yeast ferments or probiotic extracts used in existing technologies can only provide basic amino acids and nutrients, lacking the ability to immediately build a physical defense barrier on the damaged scalp surface. Second, the traditional polysaccharide ingredients used in existing formulas, due to uneven molecular weight distribution or low molecular weight, cannot quickly form a film in the wash-off system, resulting in limited deep repair effects. Third, and most challenging, is the compatibility problem. When attempting to combine highly bioactive yeast extracts with film-forming ultra-high molecular weight polysaccharides, flocculation, stratification, or inactivation easily occur in traditional shampoo and conditioner bases. Fourth, existing shampoo and conditioner bases themselves lack the ability to block chemically irritated damage, and the cleansing process still causes secondary micro-damage to the fragile scalp.

[0005] Therefore, the technical problem to be solved by this invention is to provide a scalp repair composition that can form a physical film instantly, deeply soothe inflammation at the gene level, and has excellent system stability and ultra-low irritation. This composition needs to overcome the compatibility and stability challenges of ultra-high molecular weight polysaccharides in complex shampoo and conditioner systems, fundamentally solving the problems of scalp barrier damage and microecological imbalance caused by traditional surfactants. Summary of the Invention

[0006] The purpose of this invention is to provide a scalp repair composition comprising epidermal repair polysaccharides and yeast lysate to solve the problems mentioned in the background art.

[0007] To achieve the above objectives, the present invention provides a scalp repair composition comprising epidermal repair polysaccharides and yeast lysate, comprising the following raw materials by weight percentage: Epidermal repair polysaccharides: 1%~5%; Yeast lysate: 1%~5%; PEG-5 triisostearate glyceryl acid: 1%~10%; Acceptable base for cosmetics: excess.

[0008] Furthermore, the epidermal repair polysaccharide is a microbial polysaccharide (mannan) obtained by fermentation of strain Paenibacillus sp. 1538, with a weight-average molecular weight of 6.15 × 10⁻⁶. 6 Da. This polysaccharide is mainly composed of D-mannose and D-glucose linked by β-1,3 and β-1,4 glycosidic bonds to form the main chain structure.

[0009] Furthermore, the yeast lysate is derived from Saccharomyces cerevisiae. By weight, the yeast lysate raw material contains 96.0% yeast fermentation lysate filtrate, 3.5% 1,2-pentanediol, and 0.5% 1,2-hexanediol, with an effective content ≥2g / kg and a pH value of 5.0-7.5.

[0010] Furthermore, the PEG-5 triisostearate glyceryl acid has an HLB value of 8-10, an acid value of ≤8 mgKOH / g, a saponification value of 139-159 mgKOH / g, and is a colorless to pale yellow transparent liquid at 25°C.

[0011] The present invention also provides a method for preparing the above composition, comprising the following steps: S1. Pre-preparation of epidermal repair polysaccharide: Prepare seed culture medium with purified water, inoculate Paenibacillus sp. 1538 for seed culture; adjust pH and filter, then inoculate with fermentation medium for fermentation; after obtaining fermentation broth, extract polysaccharide precipitate by precipitation and centrifugation, and then dry; redissolve the dried product in purified water, filter to obtain filtrate; add preservative system to filtrate and stir evenly to obtain epidermal repair polysaccharide.

[0012] Pre-preparation of S2 and PEG-5 glyceryl triisostearate: PEG-5 glyceryl ether and isostearic acid are added to the reactor in proportion, the temperature is slowly increased to mix the materials evenly, and then the temperature is increased to the reaction temperature to carry out a multi-stage temperature-controlled esterification reaction. After monitoring the acid value and saponification value to ensure they meet the standards, the product is discharged.

[0013] S3. Composition of the composition: Weigh PEG-5 triisostearate glyceryl acid, polyol moisturizer and appropriate amount of deionized water according to the formula mass percentage, mix them, heat to 60℃-65℃ and stir evenly to form a transparent matrix liquid; cool to below 40℃, add the epidermal repair polysaccharide and yeast lysate obtained in S1, stir evenly to a homogeneous liquid, and the scalp repair composition is obtained.

[0014] Compared with the prior art, the beneficial effects of the present invention are as follows: This scalp repair composition, which includes epidermal repair polysaccharides and yeast lysate, provides powerful synergistic repair: the epidermal repair polysaccharides have immediate film-forming properties, which can build a physical defense on the scalp surface; combined with the ability of yeast lysate to promote keratinocyte regeneration, it accelerates the repair of the scalp barrier from both inside and outside.

[0015] Anti-inflammatory and soothing effects, along with microecological regulation: Epidermal repair polysaccharides effectively inhibit the release of pro-inflammatory factors induced by surfactants and other agents, and act as prebiotics to promote the proliferation of beneficial bacteria; yeast lysate promotes DNA repair. The combination of these two ingredients exhibits a significant synergistic effect in reducing inflammation.

[0016] Excellent system gentleness and stability: Custom-synthesized PEG-5 triisostearate glyceryl acid is introduced as a nonionic surfactant / emulsifier. Its extremely low irritation completely solves the problem of traditional shampoos and conditioners exacerbating scalp damage, and its superior solubilizing properties enhance the stability of polysaccharides and yeast extracts in complex formulations. Attached Figure Description

[0017] Figure 1 The chemical structural formula of the epidermal repair polysaccharide M16 of the present invention is shown below; Figure 2 The production process flow diagram of the epidermal repair polysaccharide M16 prepared in Example 1 of this invention is shown below; Figure 3 The flowchart shows the synthesis process of PEG-5 glyceryl triisostearate in Example 2 of this invention. Figure 4 This is a bar chart comparing the effects of each test group on the number of neutrophils in a zebrafish inflammation model in Experiment Example 1 of the present invention. Figure 5 This is a line graph comparing the improvement of ASFS scores for dandruff in subjects by different compositions in Experimental Example 3 of the present invention. Detailed Implementation

[0018] The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0019] To ensure the repeatability of the embodiments of the present invention, the core raw materials and conventional excipients used in the present invention are described as follows: Epidermal repair polysaccharide: obtained by fermentation using Paenibacillus sp. 1538 strain, with a weight-average molecular weight of 6.15 × 10⁻⁶. 6 The active ingredient (polysaccharide) content is controlled at 0.9%~1.1%, the protein content is ≤0.1%, and the pH value is 5.0-8.5. Its chemical structural formula is as follows: Figure 1 As shown in the figure. This embodiment uses "Meizhitang Epidermal Repair Polysaccharide M16" produced by Nanjing Nanfangyuan Biotechnology Co., Ltd.

[0020] Yeast lysate: A lysate extract derived from *Saccharomyces cerevisiae*. By weight percentage, this raw material system consists of 96.0% yeast fermentation lysate filtrate, 3.5% 1,2-pentanediol, and 0.5% 1,2-hexanediol; its active ingredient content is ≥2 g / kg, and its pH value is 5.0-7.5. This example uses "Meizhitang yeast lysate" produced by Nanjing Nanfangyuan Biotechnology Co., Ltd.

[0021] PEG-5 triisostearate glyceryl acid: A nonionic surfactant synthesized via a multi-stage temperature-controlled esterification reaction. Technical specifications: HLB value 8-10, colorless to pale yellow transparent liquid at 25℃, acid value ≤8 mgKOH / g, saponification value 139-159 mgKOH / g.

[0022] Other common matrices and excipients: Glycerin, sodium lauryl ether sulfate (SLES), etc., used in the examples and comparative examples are all commercially available cosmetic grade raw materials; the experimental water is deionized water.

[0023] Preparation Example 1: Preparation of epidermal repair polysaccharide, the specific production process is as follows: Figure 2 As shown.

[0024] (1) Seed culture: Prepare seed culture medium with purified water (the culture medium formula is: glucose 2.0%, yeast extract 1.0%, peptone 1.0%, dipotassium hydrogen phosphate 0.1%, magnesium sulfate 0.05%, and the remainder is water. Adjust the pH to 6.8~7.2 before use), sterilize at 121℃ for 20 minutes, cool and inoculate with Paenibacillus sp.1538 strain, and culture at 30℃-32℃ and shaking speed of 150-200rpm for 18-24 hours to obtain seed liquid in the logarithmic growth phase.

[0025] (2) Fermentation culture: The above seed culture was inoculated into a fermenter containing fermentation medium at an inoculation rate of 5%-8% (volume ratio). The fermentation conditions were controlled as follows: temperature 30℃-35℃, aeration rate 0.5-1.0 vvm, and stirring speed 200-300 rpm. During the fermentation process, the pH of the system was maintained between 6.5 and 7.0 by adding acid and alkali. The fermentation time lasted for 48-72 hours until the polysaccharide concentration in the fermentation broth no longer increased significantly, and the fermentation broth was obtained.

[0026] (3) Extraction and purification: Centrifuge the fermentation broth at 5000 rpm for 15 minutes to remove the cell precipitate and collect the supernatant. Slowly add 3-4 times the volume of anhydrous ethanol to the supernatant for alcohol precipitation. Let it stand for 8-12 hours to fully extract the polysaccharide. Then, collect the polysaccharide precipitate by centrifugation, wash it with an appropriate amount of anhydrous ethanol, and dry it in a vacuum drying oven at 50℃-60℃ to constant weight to obtain the dried polysaccharide product.

[0027] (4) Resolution and preservation: The dried polysaccharide is resolution in purified water at a predetermined concentration, and the insoluble impurities are removed by filtration through a 0.22μm microporous membrane to obtain a clear filtrate; finally, 0.6% phenoxyethanol (or a mixture of 0.5% 1,2-pentanediol and 0.5% 1,2-hexanediol) is added to the filtrate as a preservative, and the mixture is stirred evenly to obtain an epidermal repair polysaccharide solution with an active ingredient content of 0.9%-1.1%.

[0028] Preparation Example 2: Preparation of PEG-5 glyceryl triisostearate, the synthetic route is as follows: Figure 3 As shown.

[0029] (1) Ingredients and mixing: In a reactor equipped with a thermometer, mechanical stirrer and condenser, PEG-5 glycerol ether and isostearic acid are precisely mixed at a molar ratio of 1:3.05 and added to the reactor (slightly excess isostearic acid to ensure complete esterification). 0.2% p-toluenesulfonic acid by mass of total materials is added as an esterification catalyst, and 0.1% hypophosphoric acid is added as an antioxidant to ensure the color of the product.

[0030] (2) Temperature-controlled esterification reaction: Start stirring and slowly heat to 120℃-140℃ under normal pressure. Keep the mixture warm for 1-2 hours to promote thorough mixing of materials and remove free water. Then, further heat to the second stage reaction temperature of 180℃-200℃ and gradually turn on the vacuum pump to control the vacuum degree of the system at -0.08MPa to -0.095MPa for dehydration esterification and heat preservation reaction.

[0031] (3) Monitoring and Discharge: During the reaction, samples are taken in real time to monitor the acid value and saponification value of the system. After the reaction has proceeded for 6-8 hours, when the acid value of the system drops to ≤8mgKOH / g and the saponification value stabilizes at 139-159mgKOH / g, heating is stopped. After the vacuum is broken, the material is cooled to 60℃-80℃ and discharged through a filter screen to obtain a high-purity colorless to pale yellow transparent PEG-5 triisostearate glyceric acid liquid.

[0032] Composition Examples 1-3 and Comparative Examples 1-3: The scalp repair composition formulations of each example and comparative example are shown in Table 1 (values ​​are mass percentages, and the balance is deionized water).

[0033] Table 1: Composition Formulation Table Comparative Example 1 lacks epidermal repair polysaccharide, Comparative Example 2 lacks yeast lysate, and Comparative Example 3 uses the conventional anionic surfactant SLES instead of the PEG-5 triisostearate of the present invention.

[0034] Preparation method of the composition (taking Example 2 as an example): S1. Preparation of matrix phase: Accurately weigh 5.0% of PEG-5 triisostearate glycerol, 5.0% of glycerol and the remainder of deionized water according to the formula ratio, add them to the emulsification pot, turn on the stirring (150-200 rpm), slowly heat to 60℃-65℃, and keep stirring until all components are dissolved evenly to form a transparent matrix liquid.

[0035] S2. Addition of active phase: Stop heating and allow the system to cool naturally. When the system temperature drops below 40°C, slowly add 3.0% of epidermal repair polysaccharide and 3.0% of yeast lysate in sequence.

[0036] S3. Homogenization and Discharge: Maintain stirring for 15-20 minutes to ensure uniform dispersion of the active ingredients. After confirming the formation of a homogeneous transparent or semi-transparent liquid, allow it to stand to defoam, then discharge and fill to obtain the final product.

[0037] The preparation methods for other embodiments and comparative examples are the same.

[0038] Experimental Example 1: Assessment of Neutrophil Inflammation Relief in Zebrafish A transgenic neutrophil-producing green fluorescent zebrafish (MPX) model was used to induce skin and mucous membrane inflammation by water-soluble administration of sodium lauryl sulfate (SLS). The number of neutrophils in the fluorescent regions was quantitatively counted. The experimental results are as follows: Figure 4 As shown.

[0039] Combination Figure 4 As can be seen, compared with the model control group, Comparative Example 1 (single yeast) and Comparative Example 2 (single polysaccharide), which use similar single active ingredients to existing technologies, although they have a certain anti-inflammatory effect, only have a neutrophil reduction rate of 12% and 25%, respectively; while with the combined effect of Example 2 of this invention (3% polysaccharide + 3% yeast compound), the number of neutrophils was significantly reduced by 41% (p<0.01). The data intuitively demonstrate that both produce extremely significant anti-inflammatory and soothing effects in repairing chemically stimulated damage, and the effect is far greater than the sum of the effects of using either ingredient alone.

[0040] Experimental Example 2: In vitro cell repair and toxicity test Chemical stimulation modeling was created by adding 0.02% SLS to human immortalized keratinocyte (HaCat) culture medium to simulate the chemical damage to the scalp caused by daily hair care products.

[0041] Experimental results showed that the cell survival rate of Comparative Example 3 (using conventional SLES surfactant) was only 42%, while the cell survival rate of Example 2 recovered to 93%. This fully demonstrates that the customized emulsion matrix selected in this invention is highly compatible with the active ingredients, overcomes the irritation defects of conventional surfactants, and can effectively resist the cytotoxicity and secondary barrier damage caused by chemical surfactants.

[0042] Experimental Example 3: Clinical Evaluation of Dandruff Removal and Itch Relief for Human Scalp Sixty participants with scalp sensitivity, dandruff, and itching were enrolled and randomly divided into three groups. They used the test sample shampoo once daily for 14 consecutive days. The ASFS (Scalp Dandruff Severity Assessment Scale) was used for scoring, and the results are as follows: Figure 5 As shown.

[0043] Combination Figure 5As can be seen, the curve in the blank control group using ordinary shampoo decreased extremely slowly over time, with the ASFS score dropping from a baseline of 2.51 to 2.30, showing almost no significant improvement. In contrast, the curves representing the compositions of this invention in Examples 1 and 3 showed a steep downward trend on days 7 and 14. In particular, Example 3, with the highest concentration of active ingredient, rapidly reduced the ASFS score from the severe dandruff range of 2.48 to the healthy range of 0.51 within 14 days, and 95% of subjects reported a reduction in scalp erythema. The trend comparison in the above charts fully demonstrates that the compositions of this invention can fundamentally solve the problems of scalp inflammation and dandruff without relying on traditional toxic chemical anti-dandruff agents, possessing strong industrial applicability.

[0044] The foregoing has shown and described the basic principles, main features, and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited to the above embodiments. The embodiments and descriptions in the specification are merely preferred examples and are not intended to limit the invention. Various changes and modifications can be made to the invention without departing from its spirit and scope, and all such changes and modifications fall within the scope of the present invention as claimed. The scope of protection of the present invention is defined by the appended claims and their equivalents.

Claims

1. A scalp repair composition comprising epidermal repair polysaccharide and yeast lysate, characterized in that, By weight percentage, it includes the following raw materials: Epidermal repair polysaccharides: 1%-5%; Yeast lysate: 1%-5%; PEG-5 triisostearate glyceryl acid: 1%-10%; Acceptable base for cosmetics: Balance; The epidermal repair polysaccharide was obtained by fermentation of Paenibacillus sp. 1538 strain with a weight-average molecular weight of 6.15 × 10⁻⁶. 6 The mannan of Da, wherein the mannan is formed by the main chain structure of D-mannose and D-glucose linked by β-1,3 and β-1,4 glycosidic bonds; the HLB value of the PEG-5 triisostearate glyceride is 8-10; The epidermal repair polysaccharide, yeast lysate, and PEG-5 triisostearate glycerate are combined to form a homogeneous system, which is used to inhibit the release of pro-inflammatory factors caused by chemical stimulation and repair the physical barrier of the scalp.

2. The scalp repair composition comprising epidermal repair polysaccharide and yeast lysate according to claim 1, characterized in that, The yeast lysate is derived from Saccharomyces cerevisiae. By mass percentage, the yeast lysate raw material contains 96.0% yeast fermentation lysate filtrate, 3.5% 1,2-pentanediol, and 0.5% 1,2-hexanediol, and the active ingredient content of the raw material is ≥2g / kg, with a pH value of 5.0-7.

5.

3. The scalp repair composition comprising epidermal repair polysaccharide and yeast lysate according to claim 1, characterized in that, The PEG-5 triisostearate glyceryl acid has an acid value ≤8 mgKOH / g, a saponification value of 139-159 mgKOH / g, and is a colorless to pale yellow transparent liquid at 25°C.

4. The scalp repair composition comprising epidermal repair polysaccharide and yeast lysate according to claim 1, characterized in that, The acceptable bases for the cosmetic include glycerin and deionized water.

5. The scalp repair composition comprising epidermal repair polysaccharide and yeast lysate according to claim 1, characterized in that, The chemical irritation specifically refers to scalp cell toxicity and barrier damage caused by surfactants in detergent cosmetics.

6. A method for preparing the scalp repair composition comprising epidermal repair polysaccharide and yeast lysate as described in any one of claims 1-5, characterized in that, Includes the following steps: S1. Pre-preparation of epidermal repair polysaccharide: Prepare seed culture medium with purified water, inoculate Paenibacillus sp. 1538 for seed culture; adjust pH and filter, then inoculate with fermentation medium for fermentation; after obtaining fermentation broth, extract polysaccharide precipitate by precipitation and centrifugation, and then dry; redissolve the dried product in purified water, filter to obtain filtrate; add preservative system to filtrate and stir evenly to obtain epidermal repair polysaccharide; Pre-preparation of S2 and PEG-5 glyceryl triisostearate: PEG-5 glyceryl ether and isostearic acid are added to the reactor in a molar ratio, the temperature is slowly increased to mix the materials evenly, and then the temperature is increased to the reaction temperature to carry out a multi-stage temperature-controlled dehydration esterification reaction. After monitoring the acid value and saponification value to ensure they meet the standards, the product is discharged. S3. Composition of the composition: Weigh PEG-5 triisostearate glyceryl acid, polyol moisturizer and the remaining deionized water according to the formula mass percentage, mix them, heat to 60℃-65℃ and stir evenly to form a transparent matrix liquid; cool to below 40℃, add the epidermal repair polysaccharide and yeast lysate obtained in S1, stir evenly to a homogeneous liquid, and the scalp repair composition is obtained.

7. The method for preparing the scalp repair composition comprising epidermal repair polysaccharide and yeast lysate according to claim 6, characterized in that, In S1, the fermentation process is as follows: the seed liquid obtained from seed culture is inoculated into a fermenter containing fermentation medium at a volume ratio of 5%-8%, and fermentation is carried out at a temperature of 30℃-35℃, an aeration rate of 0.5-1.0 vvm, and a stirring speed of 200-300 rpm. During the fermentation process, acid and alkali are added to maintain the pH of the system between 6.5 and 7.0, and the fermentation time is 48-72 hours.

8. The method for preparing the scalp repair composition comprising epidermal repair polysaccharide and yeast lysate according to claim 6, characterized in that, In step S1, the process of extracting polysaccharide precipitate by precipitation and centrifugation and then drying is as follows: the fermentation broth is centrifuged at 5000 rpm for 15 minutes to collect the supernatant, 3-4 times the volume of anhydrous ethanol is added for alcohol precipitation, and after standing for 8-12 hours, the polysaccharide precipitate is collected by centrifugation, washed with anhydrous ethanol, and then placed in a vacuum drying oven at 50℃-60℃ to dry to constant weight.

9. The method for preparing the scalp repair composition comprising epidermal repair polysaccharide and yeast lysate according to claim 6, characterized in that, In step S2, PEG-5 glycerol ether and isostearic acid are added to the reactor at a molar ratio of 1:3.05, along with 0.2% p-toluenesulfonic acid and 0.1% hypophosphoric acid by mass of the total materials. The multi-stage temperature-controlled dehydration esterification reaction process is as follows: the temperature is raised to 120℃-140℃ under normal pressure and kept at this temperature for 1-2 hours, followed by raising the temperature to 180℃-200℃, and the system vacuum is controlled between -0.08MPa and -0.095MPa for a dehydration esterification reaction held at this temperature for 6-8 hours.

10. The method for preparing the scalp repair composition comprising epidermal repair polysaccharide and yeast lysate according to claim 6, characterized in that, In step S3, the stirring speed is set to 150-200 rpm when mixing PEG-5 triisostearate glyceryl acid, polyol moisturizer and deionized water; after cooling and adding epidermal repair polysaccharide and yeast lysate, the stirring time is maintained for 15-20 minutes.

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