Detection device based on light-excited chemiluminescence

Abstract

The embodiment of the invention discloses a detection device based on light-excited chemiluminescence. The detection device comprises a detection seat, the sample injection mechanism is arranged on the detection seat, and the sample injection mechanism is arranged corresponding to the feeding groove; the uniform mixing mechanism is arranged on the detection seat; the incubation mechanism is arranged on the detection base and arranged on one side of the uniform mixing mechanism, the incubation mechanism is of an arc-shaped structure, and the incubation mechanism is further provided with a discharging groove; the detection mechanism is arranged on the detection seat and is arranged on one side of the incubation mechanism; the box taking and placing mechanism is arranged on the detection seat, and the box taking and placing mechanism is used for transferring the reagent card box; wherein the feeding mechanism, the uniform mixing mechanism, the incubation mechanism and the detection mechanism are all located in a box taking and placing working range of the box taking and placing mechanism. According to the scheme, a portable automatic structural design is adopted, equipment is small in size and convenient to transport and carry, equipment operation does not need manual intervention, and time and labor are saved.

Description

Technical Field

[0001] This invention relates to the technical field of immunoassay, and in particular to a detection device based on photo-induced chemiluminescence. Background Technology

[0002] Chemiluminescence immunoassay is an analytical method that combines chemiluminescence detection technology with the immune system to detect antigens or antibodies. By using homogeneous chemiluminescence detection technology, photosensitive nanospheres are coupled to one antibody involved in the immune reaction, and luminescent nanospheres containing dimethylthiophene derivatives and Eu chelates are coupled to another antibody. This method has both the specificity of the immune reaction and the high sensitivity of the chemiluminescence reaction, and has therefore been widely used in clinical practice.

[0003] A chemiluminescence immunoassay analyzer is an automated device that controls the immunochemical reaction process outside the human body and detects and analyzes the reaction process and results. The photo-induced chemiluminescence immunoassay analyzer is used in conjunction with a microfluidic reagent cartridge. During use, the components of the reagents required for the experimental detection process are directly freeze-dried into microspheres and placed into the corresponding positions in the reagent cartridge. The movement of the cartridge's grippers enables operations such as barcode recognition, cartridge capping, and incubation detection, achieving control of the reaction process and automatic detection and analysis of the reaction results.

[0004] However, chemiluminescence immunoassay analyzers in related technologies are usually bulky, occupy a lot of installation space, and have great limitations in application. In addition, the internal liquid circuit structure of the equipment requires regular maintenance, which involves waste liquid treatment, and the maintenance operation is complex and costly. Summary of the Invention

[0005] In view of this, this invention patent provides a detection device based on photo-induced chemiluminescence to solve the problems of large space occupation, complex structure, cumbersome operation and maintenance, time and labor consumption, and high cost in the prior art.

[0006] To achieve one, some, or all of the above objectives, or other objectives, the present invention provides a detection device based on photo-induced chemiluminescence, comprising: Test socket; A sample injection mechanism is disposed on a detection seat and is disposed corresponding to a feed trough; A mixing mechanism is disposed on the detection seat; An incubation mechanism is provided, which is disposed on the detection seat and arranged on one side of the mixing mechanism, and the incubation mechanism has an arc-shaped structure and is also provided with a discharge trough. A testing mechanism, wherein the testing mechanism is disposed on the testing seat and arranged on one side of the incubation mechanism; and A box picking and placing mechanism is disposed on the detection seat and is used to transfer reagent boxes; wherein, the feeding mechanism, the mixing mechanism, the incubation mechanism and the detection mechanism are all within the box picking and placing working range of the box picking and placing mechanism.

[0007] In one embodiment, the detection mechanism includes a darkroom module, a detection slot module, a connecting sleeve, a photon counting unit, a detection mechanism control board, a side plate, a photon counting unit fixing plate, and a base plate. The detection slot module and the darkroom module are connected via the connecting sleeve. The darkroom module is mounted on one side of the side plate, and the photon counting unit is mounted on the other side of the side plate via the photon counting unit fixing plate. The detection mechanism control board is mounted on the side plate and arranged on the same side as the photon counting unit. The side plate is mounted on the detection base. The darkroom module is used to perform detection operations on the reagent cartridges in the detection slot module.

[0008] In one embodiment, the darkroom module includes a detection PMT assembly, a darkroom shutter, a receiving light lens, an excitation light source, an excitation light lens, a dichroic mirror, a lens group, a darkroom box, a darkroom box cover, and a darkroom box cover fixing plate. The excitation light source, the excitation lens, the dichroic mirror, the receiving lens, the darkroom shutter, and the lens are assembled on the darkroom cover plate. The detection PMT assembly is installed at the slot of the darkroom. The darkroom cover plate is connected to the darkroom via the darkroom cover plate fixing plate.

[0009] In one embodiment, the incubation mechanism includes an incubation tray, a heating belt, a temperature sensor, an over-temperature protection switch, an incubation tray support column, and insulation cotton. The incubation tray has multiple units arranged in an arc shape. The incubation tray is used to load reagent cartridges and incubate the reagent cartridges at a constant temperature through thermal conduction. The heating belt is disposed on the side of the incubation tray to wrap the incubation tray. The temperature sensor is electrically connected to the incubation tray. The over-temperature protection switch is disposed on the incubation tray. The incubation tray support column is disposed on the bottom surface of the incubation tray. The insulation cotton is fitted over the outside of the incubation tray. A discharge trough is provided in the middle of the incubation tray.

[0010] In one embodiment, the mixing mechanism includes a reagent cartridge transfer power source, a mixing fixing seat, a vibration module, a vibration module fixing plate, a card pressing driver, a card pressing pin, a card pressing top plate, and a mixing mechanism fixing bracket. The card pressing pin is connected to the card pressing top plate, and the card pressing top plate is mounted on the card pressing driver. The card pressing driver is mounted on the upper part of the mixing mechanism fixing bracket and is used to press the reagent cartridge and limit its movement during the mixing process. The vibration module is disposed at the lower part of the mixing mechanism fixing bracket. The mixing fixing seat is connected to the reagent cartridge transfer power source through an L-shaped connecting plate. The mixing fixing seat is used to load the reagent cartridge and is disposed at the lower part of the mixing mechanism fixing bracket. The mixing fixing seat is located on one side of the vibration module.

[0011] In one embodiment, the box picking and placing mechanism includes an XY plane rotation drive module, a Z-axis lifting drive module, a box picking and placing gripper, a rotating arm, and a box picking and placing fixed bracket. The XY plane rotation drive module is mounted on the upper part of the box picking and placing fixed bracket, the Z-axis lifting drive module is mounted on the lower part of the box picking and placing fixed bracket, the box picking and placing gripper is mounted on the rotating arm, and the rotating arm is mounted on the rotation axis of the box picking and placing fixed bracket. The XY plane rotation drive module is connected to the gear mounted on the rotation shaft via a rack, and the Z-axis lifting drive module is connected to the idler wheel mounted on the drive module mounting bracket via a rack.

[0012] In one embodiment, the box picking and placing mechanism further includes a light shield, which is mounted on the box picking and placing gripper. The light shield is used by the box picking and placing gripper to pick up the reagent card box and transfer it to the photometric module. During photometric measurement, it forms a darkroom environment with the photometric slot to avoid the influence of ambient light on the detection results.

[0013] In one embodiment, the sample feeding mechanism includes a motor mounting base, a linear motor drive module, a feed trough, a material cup sensor, a first photoelectric sensor, a second photoelectric sensor, a photoelectric sensor baffle, and a photoelectric sensor mounting plate; The feed trough is used to hold reagent card boxes for testing; the motor mounting base is set on the testing seat and connected to the linear motor drive module; the material cup sensor is installed on the back of the feed trough; the feed trough is connected to the slider on the linear motor drive module through a connecting plate; the first photoelectric sensor and the second photoelectric sensor are respectively installed at both ends of the photoelectric sensor mounting plate and located on one side of the linear motor drive module; the photoelectric sensor baffle is installed on the slider of the linear motor drive module and is used to trigger and cooperate with the first photoelectric sensor and the second photoelectric sensor.

[0014] In one embodiment, the detection base includes a base plate, a side plate, a circuit board mounting plate, a power socket, and a data socket; The side plate is mounted on the base plate, the power socket is mounted on the side plate and used to connect to an external power source, the data socket is mounted on the side plate and used to connect to an external device, and the circuit board fixing plate is connected to the upper part of the side plate. A control host is also installed on the side panel, and a main control board is provided on the circuit board fixing plate.

[0015] In one embodiment, the detection base further includes elastic support feet, and four elastic support feet arranged in a rectangular pattern are fixed to the bottom of the base plate.

[0016] Implementing the embodiments of the present invention will have the following beneficial effects: In this photo-induced chemiluminescence-based detection device, the sample introduction mechanism, mixing mechanism, incubation mechanism, detection mechanism, and cartridge handling mechanism are integrated and installed in the detection seat. The compact layout of each functional module makes the entire detection device small in size, light in weight, and occupies little installation space, thus having a wider range of applications. During the detection operation, each functional module can automatically coordinate and cooperate under the command of the central controller. Specifically, the reagent cartridge containing the lyophilized microspheres and the sample is loaded into the sample introduction slot, and the sample introduction mechanism transfers the reagent cartridge into the detection device. The reagent cartridge handling mechanism transfers the reagent cartridge from the sample inlet to the mixing mechanism, where the reagent and sample are thoroughly mixed. Then, the mechanism transfers the cartridge to the incubation mechanism, where it heats and maintains the cartridge at a set temperature. Next, the mechanism transfers the cartridge, now at the appropriate temperature, from the incubation mechanism to the detection mechanism, where it tests the cartridge and reads the results. Finally, the mechanism transfers the tested cartridge to the discharge trough and out of the detection device, thus completing the entire automated detection process. Since it does not involve a liquid path structure, there is no need for liquid path maintenance or waste disposal, making operation convenient and simple, with low testing costs, no human intervention, and saving time and effort. Attached Figure Description

[0017] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0018] in: Figure 1 This is a schematic diagram of the assembly structure of a photo-induced chemiluminescence-based detection device according to one embodiment; Figure 2 for Figure 1 A schematic diagram of the exploded structure; Figure 3 This is a schematic diagram of the detection mechanism described in one embodiment; Figure 4 for Figure 3 A schematic diagram of the exploded structure; Figure 5 for Figure 3 Exploded view of the anechoic chamber module; Figure 6 This is a schematic diagram of the assembly structure of the incubation mechanism according to one embodiment; Figure 7 for Figure 6 A schematic diagram of the exploded structure; Figure 8 This is a schematic diagram of the assembly structure of the mixing mechanism described in one embodiment; Figure 9 for Figure 8 A schematic diagram of the exploded structure; Figure 10 This is a schematic diagram of the assembly structure of the sample injection mechanism according to one embodiment; Figure 11 for Figure 10 A schematic diagram of the exploded structure; Figure 12 This is a schematic diagram of the assembly structure of the box loading and unloading mechanism according to one embodiment; Figure 13 for Figure 12 A schematic diagram of the exploded structure; Figure 14 This is a schematic diagram of the assembly structure of the detection seat according to one embodiment; Figure 15 for Figure 14 A schematic diagram of the explosion structure.

[0019] Explanation of reference numerals in the attached figures: 100. Detection devices based on photo-induced chemiluminescence; 10. Detection base; 11. Base plate; 12. Side plate; 13. Circuit board mounting plate; 14. Flexible support feet; 15. Data socket; 16. Control host; 17. Main control board; 20. Sample infeeding mechanism; 21. Slider; 22. Linear motor drive module; 23. Material cup holder; 24. Material cup sensor; 25. First photoelectric sensor; 26. Second photoelectric sensor; 27. Photoelectric sensor baffle; 28. Photoelectric sensor fixing plate; 29. ​​Support column; 29a. Adapter plate; 30. Mixing mechanism; 31. Reagent card holder transfer power source; 32. Mixing fixing base; 33. Vibration module; 34. Vibration module fixing plate; 35. Card pressing driver; 36. Card pressing top plate; 37. Mixing mechanism fixing bracket; 38. Card pressing pin; 39. L-shaped connecting plate; 40. Incubation mechanism; 41. Incubation tray cover; 42. Incubation tray; 43. Incubation tray base plate; 44. Reagent card holder limiting piece; 45. Incubation tray support column; 46. Temperature sensor; 47. Insulation cotton; 48. Heating belt; 49. Temperature control plate; 49a. Discharge trough; 50. Testing mechanism; 51. Darkroom module; 511. Testing PMT assembly; 512. Connecting sleeve; 513. Darkroom shutter; 514. Receiving lens; 515. Excitation source; 516. Excitation lens; 517. Dichroic mirror; 518. Lens group; 519. Dark box; 519a. Dark box cover; 519b. Dark box cover fixing plate; 52. Testing slot module; 53. Photon counting unit; 54. Testing mechanism control board; 55. Side plate; 56. Photon counting unit fixing plate; 57. Testing base; 60. Box picking and placing mechanism; 61. XY plane rotation drive module; 62. Z-axis lifting drive module; 63. Box picking and placing gripper; 64. Light shield; 65. Rotating arm; 66. Box picking and placing fixed bracket; 67. Rack; 68. Third photoelectric sensor baffle; 69. Rotary gear module; 69a. Rotating shaft; 69b. Fixed block; 69c. Third photoelectric sensor; 69d. Fourth photoelectric sensor; 69e. Fourth photoelectric sensor baffle; 200. Reagent card box. Detailed Implementation

[0020] To facilitate understanding of the present invention, a more complete description will be given below with reference to the accompanying drawings. Preferred embodiments of the invention are shown in the drawings. However, the invention can be implemented in many different forms and is not limited to the embodiments described herein. Rather, these embodiments are provided to provide a thorough and complete understanding of the disclosure of the invention.

[0021] It should be noted that when an element is referred to as being "fixed to" another element, it can be directly attached to the other element or there may be an intervening element. When an element is referred to as being "connected to" another element, it can be directly connected to the other element or there may be an intervening element. The terms "vertical," "horizontal," "left," "right," and similar expressions used herein are for illustrative purposes only and do not represent the only possible implementation.

[0022] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains. The terminology used herein in the description of the invention is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. The term "and / or" as used herein includes any and all combinations of one or more of the associated listed items.

[0023] like Figure 1 and Figure 2 As shown, a photo-induced chemiluminescence-based detection device 100 is described in one embodiment. It includes a detection seat 10, a sample injection mechanism 20, a mixing mechanism 30, an incubation mechanism 40, a detection mechanism 50, and a box removal and placement mechanism 60. The detection seat 10 is formed as a cubic frame structure with an internal mounting cavity. The mounting cavity can be used to accommodate the sample injection mechanism 20, the mixing mechanism 30, the incubation mechanism 40, the detection mechanism 50, and the box removal and placement mechanism 60, so as to realize the integrated installation of the sample injection mechanism 20, the mixing mechanism 30, the incubation mechanism 40, the detection mechanism 50, and the box removal and placement mechanism 60.

[0024] More specifically, with Figure 1 From the perspective of the box placement mechanism 60, the box placement mechanism 60 is arranged in the middle of the detection seat 10, and the incubation mechanism 40 is installed on the detection seat 10 and located to the left of the box placement mechanism 60.

[0025] The testing mechanism 50 is installed on the testing base 10 and is located behind the box handling mechanism 60.

[0026] The sample injection mechanism 20 is installed on the base plate 11 of the detection seat 10 and is located in front of the box loading and unloading mechanism 60.

[0027] The mixing mechanism 30 is installed on the base plate 11 of the detection seat 10 and is located in front of the box picking and placing mechanism 60.

[0028] The sample injection mechanism 20 is positioned between the incubation mechanism 40 and the mixing mechanism 30. The travel range of the box loading and unloading mechanism 60 covers the mixing mechanism 30, the sample injection mechanism 20, the incubation mechanism 40, and the detection mechanism 50.

[0029] Implementing the embodiments of the present invention will have the following beneficial effects: In the photo-induced chemiluminescence-based detection device 100 of this solution, the sample introduction mechanism 20, mixing mechanism 30, incubation mechanism 40, detection mechanism 50, and cartridge placement mechanism 60 are integrated and installed in the detection base 10. The functional modules are compactly arranged, making the entire detection device small in size and light in weight, occupying little installation space, and having a wider range of application scenarios. During the detection operation, each functional module can automatically cooperate under the command of the central controller. Specifically, the reagent cartridge 200 containing the lyophilized microspheres and the sample is loaded into the sample introduction mechanism 20 and enters the detection device through the sample introduction mechanism 20; then, the cartridge placement mechanism 60 transfers the reagent cartridge 200 to the mixing mechanism 30, where the reagent and sample are thoroughly mixed evenly, and then the cartridge placement mechanism 60 is used to transfer the reagent cartridge 200 to the mixing mechanism 30. Mechanism 60 transfers reagent cartridge 200 to incubation mechanism 40, where it heats and incubates the reagent cartridge 200 at a set temperature. Then, cartridge handling mechanism 60 transfers the reagent cartridge 200, now at the appropriate temperature, from incubation mechanism 40 to detection mechanism 50. Detection mechanism 50 tests the reagent cartridge 200 and reads the results. Finally, cartridge handling mechanism 60 transfers the tested reagent cartridge 200 to the discharge trough 49a of incubation mechanism 40, and the reagent cartridge 200 is then discharged from the detection device through discharge trough 49a. This completes the entire automatic detection process. It is evident that since the entire machine and detection process do not involve a liquid path structure, there is no need for liquid path structure maintenance or waste liquid treatment, making operation convenient and simple, with low detection costs, no human intervention required, and saving time and effort.

[0030] like Figures 3 to 5 As shown, in one embodiment, the detection mechanism 50 includes a darkroom module 51, a detection slot module 52, a photon counting unit 53, a detection mechanism control board 54, a side plate 55, a photon counting unit fixing plate 56, and a detection base 57. The detection slot module 52 is connected to the darkroom module 51 via a connecting sleeve 512, and the darkroom module 51 is mounted on one side of the side plate 55. The photon counting unit 53 is mounted on the other side of the side plate 55 via the photon counting unit fixing plate 56, and the detection mechanism control board 54 is mounted on the side plate 55, on the same side as the photon counting unit 53. The side plate 55 is mounted on the detection base 57. The detection base 57 is used to assemble the detection mechanism 50 onto the detection seat 10. The darkroom module 51 is used to perform detection operations on the reagent cartridges in the detection slot module 52.

[0031] The darkroom module 51 comprises a detection PMT assembly 511, a connecting sleeve 512, a darkroom shutter 513, a receiving light lens 514, an excitation light source 515, an excitation light lens 516, a dichroic mirror 517, a lens group 518, a darkroom cassette 519, a darkroom cover 519a, and a darkroom cover fixing plate 519b. The excitation light source 515, excitation light lens 516, dichroic mirror 517, receiving light lens 514, darkroom shutter 513, and lens group 518 are mounted on the darkroom cover 519a. The detection PMT assembly 511 is mounted in the slot of the darkroom 519, and the darkroom cover 519a is connected to the darkroom 519 via the darkroom cover fixing plate 519b.

[0032] After the reagent cartridge 200 is placed into the reagent tank, the light shield 64 on the cartridge placement mechanism 60 and the detection tank module 52 form a darkroom environment to prevent ambient light from entering the reagent tank and causing interference, thus affecting the detection accuracy. The excitation light source 515 emits excitation light, which passes through the excitation light lens 516 and then through the dichroic mirror 517 to illuminate the corresponding detection area of ​​the reagent cartridge 200, generating corresponding receiving light. The receiving light is reflected by the dichroic mirror 517 and then passes through the receiving light lens 514 to reach the detection PMT component 511 for data acquisition.

[0033] During the inspection operation, the inspection mechanism control board 54 controls the excitation light source 515 to emit excitation light and simultaneously controls the opening and closing of the darkroom door to open or close the inspection shutter. When the inspection shutter is open, the photon counting unit 53 can receive the excitation light propagating from the receiving excitation light lens 516, thereby acquiring the corresponding optical data. Afterwards, the inspection mechanism 50 interacts with the host computer using the acquired optical data and executes the inspection tasks assigned by the host computer.

[0034] like Figure 6 and Figure 7 As shown, in another embodiment, the incubation mechanism 40 includes an incubation tray cover 41, an incubation tray 42, an incubation tray base plate 43, a reagent cartridge limiting piece 44, an incubation tray support column 45, a temperature sensor 46, insulation cotton 47, a heating belt 48, and a temperature control plate 49. The incubation tray 42 has multiple trays arranged in an arc shape and is used to hold the reagent cartridges 200. A discharge trough 49a is provided in the center of the incubation tray 42, which is used to transfer the reagent cartridges 200 after testing out of the testing device. A heating belt 48 is attached to the outside of the incubation tray 42 to heat the reagent cartridges 200 in the groove. Insulation cotton 47 is fitted around the heating belt 48 to prevent heat loss into the environment and ensure the heating capacity of the heating belt 48. The incubation tray cover plate 41 and the incubation tray base plate 43 are used to fix the incubation tray 42, and are fixed to the base plate 11 of the detection seat 10 by the incubation tray support column 45. In addition, the reagent card holder limiting piece 44 is fixedly installed in the incubation tray 42 by the incubation tray base plate 43.

[0035] Because the incubation tray 42 has multiple grooves, it can simultaneously accommodate multiple reagent cartridges 200, increasing the throughput of detection. Multiple reagent cartridges 200 can undergo incubation reactions simultaneously, thus enabling multiple tests to be performed in a continuous flow. The reagent cartridge limiting piece 44 is used to limit the reagent cartridges 200, ensuring that the reagent cartridges 200 are in close contact with the grooves, improving heat conduction efficiency. The heating band 48 is used to generate heat to heat the incubation tray 42. The temperature sensor 46 is used to collect the real-time temperature of the incubation tray 42, providing temperature feedback to the temperature control board 49 for easy temperature control. The temperature control board 49 is used to control the temperature of the heating band 48 and to cut off the heating function when the protection temperature is exceeded to avoid hardware failure. The incubation tray support column 45 is used to install and fix the incubation mechanism 10 onto the detection seat 10. The insulation cotton 47 is used to maintain the temperature of the incubation mechanism 40, reduce heat loss, and ensure insulation effect. The discharge chute 49a is used to transfer the tested reagent cartridges 200 out of the detection device.

[0036] like Figure 8 and Figure 9 As shown, in one embodiment, the mixing mechanism 30 includes a reagent cartridge transfer power source 31, a mixing fixing seat 32, a vibration module 33, a vibration module fixing plate 34, a card pressing driver 35, a card pressing top plate 36, and a mixing mechanism fixing bracket 37. A card pressing pin 38 is connected to an L-shaped connecting plate 39. The card pressing top plate 36 is mounted on the card pressing driver 35, which is mounted on the upper part of the mixing mechanism fixing bracket 37, and is used to press the reagent cartridge 200 and limit its movement during the mixing process. The vibration module 33 is disposed at the lower part of the mixing mechanism fixing bracket 34. The mixing fixing seat 32 is connected to the reagent cartridge transfer power source 31 via the L-shaped connecting plate 39. The mixing fixing seat 32 is used to load the reagent cartridge 200 and is disposed at the lower part of the mixing mechanism fixing bracket 37, with the mixing fixing seat 32 located on one side of the vibration module 33.

[0037] During testing, the reagent cartridge 200 is transferred from the sample injection mechanism 20 to the mixing and fixing seat 32 by the cartridge handling mechanism 60. The reagent cartridge transfer power source 31 then moves the reagent cartridge 200 above the vibration module 33. The cartridge pressing driver 35 then presses the reagent cartridge 200 together and maintains a certain vibration space. The vibration module 33 is then activated, and the vibration motor applies vibration to the mixing and fixing seat 32, ensuring that the reagent and sample within the reagent cartridge 200 are more thoroughly and evenly mixed, thereby improving the accuracy of subsequent test results.

[0038] like Figure 10 and Figure 11As shown, in one embodiment, the sample injection mechanism 20 includes a motor mounting base, a slider 21, a linear motor drive module 22, a sample cup holder 23, a sample cup sensor 24, a first photoelectric sensor 25, a second photoelectric sensor 26, a photoelectric sensor baffle 27, a photoelectric sensor mounting plate 28, a support column 29, and an adapter plate 29a. The sample cup holder 23 is used to hold the reagent card box 200 for detection.

[0039] A motor mounting base is mounted on the detection seat 10 and connected to the linear motor drive module 22. A cup sensor 24 is mounted on the back of a cup support 23, which is connected to a slider 21 on the linear motor drive module 22 via an adapter plate 29a. The first photoelectric sensor 25 and the second photoelectric sensor 26 are respectively mounted at both ends of the photoelectric sensor mounting plate 28 and on one side of the linear motor drive module 22. A photoelectric sensor baffle 27 is mounted on the slider 21 of the linear motor drive module 22 and is used in conjunction with the first photoelectric sensor 25 and the second photoelectric sensor 26 for triggering.

[0040] During the testing process, the user places the reagent cartridge 200, after sample addition, into the cup holder 23. The cup sensor 24 on the back of the cup holder 23 detects the reagent cartridge 200 and sends a feedback signal to the linear motor drive module 22. The motor then moves the cup holder 23 and the reagent cartridge 200 into the testing device. When the second photoelectric sensor 26 is triggered by being blocked by the photoelectric sensor baffle 27, a feedback signal is sent to the linear motor drive module 22, and the motor stops outputting power. When the cartridge pick-up / placement gripper 63 removes the reagent cartridge 200 from the cup holder 23, the cup sensor 24 detects the absence of the reagent cartridge 200 and sends a feedback signal to the linear motor drive module 22. The motor then moves the cup holder 23 outside the testing device, awaiting the next sample addition. When the first photoelectric sensor 25 is triggered by being blocked by the photoelectric sensor baffle 27, a feedback signal is sent to the linear motor drive module 22, and the motor stops outputting power. The first photoelectric sensor 25 and the second photoelectric sensor 26 are used to limit the movement of the motor.

[0041] like Figure 12 and Figure 13 As shown, in one embodiment, the box picking and placing mechanism 60 includes an XY plane rotation drive module 61, a Z-axis lifting drive module 62, a box picking and placing gripper 63, a light shield 64, a rotating arm 65, a box picking and placing fixing bracket 66, a rack 67, a third photoelectric sensor baffle 68, a rotating gear module 69, a rotating shaft 69a, a fixing block 69b, a third photoelectric sensor 69c, a fourth photoelectric sensor 69d, and a fourth photoelectric sensor baffle 69e.

[0042] The XY plane rotation drive module 61 is mounted on the upper part of the box picking and placing fixing bracket 66, and the Z-axis lifting drive module 61 is mounted on the lower part of the box picking and placing fixing bracket 66. The light shield 64 is mounted on the box picking and placing gripper 63. The light shield 64 is used by the box picking and placing gripper 63 to grasp the reagent card box 200 and transfer it to the detection mechanism 50. During photometry, it forms a dark chamber environment with the detection slot module 52 to avoid the influence of ambient light on the detection results. The box picking and placing gripper 63 is mounted on the rotating arm 65, and the rotating arm 65 is mounted on the rotating shaft 69a on the box picking and placing fixing bracket 66. The synchronous wheel of the XY plane rotation drive module 61 is connected to the rotating gear module 69 mounted on the rotating shaft through the rack 67. A baffle 68 is fixed above the rotating gear module 69, which is triggered in conjunction with the corresponding third photoelectric sensor 69c. The synchronous pulley of the Z-axis lifting drive module 62 is connected to an idler wheel mounted on the drive module mounting bracket 66 via a rack 67. The rack 67 is connected to a fixed block 69b on the rotating shaft 69a. Another fourth photoelectric sensor baffle 69e is fixed on the rack 67, which cooperates with the corresponding fourth photoelectric sensor 69d to trigger the sensor. Both photoelectric switches are mounted on the box pick-up and drop-off mounting bracket 66.

[0043] During the testing process, with the help of the XY plane rotation drive module 61 and the Z-axis lifting drive module 62, the box pick-and-place gripper 63 can rotate in a circular motion and move up and down flexibly within the sample injection mechanism 20, mixing mechanism 30, incubation mechanism 40 and testing mechanism 50, so as to flexibly transfer the reagent box 200 between different functional modules and ensure that the testing process is orderly and automatically completed.

[0044] Specifically, the XY-plane rotary drive module 61 consists of a stepper motor and a synchronous pulley. The synchronous pulley is mounted on the motor shaft of the stepper motor and is connected to a rotary module gear 69 mounted on a box-picking and placing bracket 66 via a rack 67. The rotary module gear 69 is mounted on a rotating shaft 69a. One end of the rotating arm 65 is mounted on the upper part of the rotating shaft 69a, and the other end is fixed to a box-picking and placing gripper 63. The stepper motor of the XY-plane rotary drive module 61 drives the rack 67 via the synchronous pulley, which in turn drives the rotary module gear 69 to rotate, thereby causing the rotating arm 65 and the box-picking and placing gripper 63 to rotate. A third photoelectric sensor 69c is fixed to one side of the box-picking and placing bracket 66, and a third photoelectric sensor baffle 68 is fixed above the rotary module wheel for limiting the movement of the stepper motor hardware of the XY-plane rotary drive module 61.

[0045] The Z-axis lifting drive module 62 consists of a stepper motor and a synchronous pulley, with the synchronous pulley mounted on the stepper motor. The stepper motor is located below the XY plane rotary drive module 61 and is fixed to the box pick-and-place fixing bracket 66. The synchronous pulley is connected to an idler gear mounted on the drive module fixing bracket 66 via a rack 67, and the rack 67 is connected to the rotating shaft 69a via a fixing block 69b. The stepper motor of the Z-axis lifting drive module 62 drives the rack 67 to move via the synchronous pulley. The rack drives the fixing block 69b mounted above to move up and down, thereby driving the rotating arm 65 and the box pick-and-place gripper 63 to move up and down. A fourth photoelectric sensor baffle 69e is fixed on the fixing block 69b, and a fourth photoelectric sensor 69d is fixed at a corresponding position on the drive module bracket 66, used to limit the movement of the stepper motor hardware of the Z-axis lifting drive module 62.

[0046] A light shield 64 is fixed below the box pick-and-place gripper 63. During the testing operation, the box pick-and-place mechanism 60 picks up the incubated reagent card box 200 and transfers it to the testing slot module 52 of the testing mechanism 50. At this time, the light shield 64 on the box pick-and-place mechanism 50 can form a dark chamber environment with the testing slot module 52 of the testing mechanism 50 to avoid the influence of ambient light on the testing results.

[0047] like Figure 14 and Figure 15 As shown, in one embodiment, the detection base 10 includes a base plate 11, side plates 12, a circuit board fixing plate 13, elastic support feet 14, a power socket 15, a data socket 16, and a main control board 17. A box-loading gripper and box-loading fixing bracket 66 is mounted on the base plate 11 for transferring the reagent card box 200 between modules. An incubation tray support column 45 is mounted on the base plate 11 for fixing the incubation mechanism 40. At least two elastic support feet 14 are distributedly installed at the bottom of the base plate 11 to stably support the detection device in the usage area.

[0048] The elastic support foot 14 includes a first telescopic rod assembly, a second telescopic rod assembly, an elastic element, and a foot cup. Both the first and second telescopic rod assemblies include a first connecting rod and a second connecting rod hinged to each other. The end of the first connecting rod away from the second connecting rod is rotatably connected to the base plate via a first pin, and the end of the second connecting rod away from the first connecting rod is rotatably connected to the foot cup via a second pin. The elastic element connects the base plate and the foot cup. The first and second telescopic rod assemblies are symmetrically distributed on opposite sides of the elastic element and the foot cup. This arrangement connects the foot cup and the base plate into a single structure. Furthermore, the rotational freedom provided between the first and second connecting rods, between the first and second connecting rods and the base plate, and between the second connecting rod and the foot cup, along with the telescopic force provided by the elastic element, enables the overall elastic support foot 14 to adaptively float and rise. This improves the adaptability of the testing device to different site conditions and prevents structural damage from external impacts.

[0049] Side plate 12 is mounted on base plate 11. Power socket 15 is located on side plate 12 and is used to connect to an external power source for powering the entire testing device. Data socket 16 is located on side plate 12 and is used to connect to external devices for information exchange. A control host is also fixed on side plate 12. Circuit mounting plate 13 is mounted on side plate 12, and a main control board 17 is mounted on mounting plate 13. The control host controls the main control board 17 and processes and displays the data uploaded by each control board, ensuring the testing process is automatic and orderly. The main control board 17 is used to execute control programs related to motor drive control, sensor monitoring, reading control, power control, and interaction with the host within the testing device.

[0050] The technical features of the above embodiments can be combined in any way. For the sake of brevity, not all possible combinations of the technical features in the above embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0051] The embodiments described above are merely illustrative of several implementations of the present invention, and while the descriptions are relatively specific and detailed, they should not be construed as limiting the scope of the invention patent. It should be noted that those skilled in the art can make various modifications and improvements without departing from the concept of the present invention, and these all fall within the protection scope of the present invention. Therefore, the protection scope of this invention patent should be determined by the appended claims.

Claims

1. A detection device based on photo-induced chemiluminescence, characterized in that, include: Test socket; A sample injection mechanism is disposed on a detection seat and is disposed corresponding to a feed trough; A mixing mechanism is disposed on the detection seat; An incubation mechanism is provided, which is disposed on the detection seat and arranged on one side of the mixing mechanism, and the incubation mechanism has an arc-shaped structure and is also provided with a discharge trough. A testing mechanism, wherein the testing mechanism is disposed on the testing seat and arranged on one side of the incubation mechanism; and A box picking and placing mechanism is disposed on the detection seat and is used to transfer reagent boxes; wherein, the feeding mechanism, the mixing mechanism, the incubation mechanism and the detection mechanism are all within the box picking and placing working range of the box picking and placing mechanism.

2. The detection device based on photo-induced chemiluminescence as described in claim 1, characterized in that, The detection mechanism includes a darkroom module, a detection slot module, a connecting sleeve, a photon counting unit, a detection mechanism control board, a side plate, a photon counting unit fixing plate, and a base plate. The detection slot module and the darkroom module are connected by the connecting sleeve. The darkroom module is mounted on one side of the side plate, and the photon counting unit is mounted on the other side of the side plate via the photon counting unit fixing plate. The detection mechanism control board is mounted on the side plate and arranged on the same side as the photon counting unit. The side plate is mounted on the detection base. The darkroom module is used to perform detection operations on the reagent cartridges in the detection slot module.

3. The detection device based on photo-induced chemiluminescence as described in claim 2, characterized in that, The darkroom module includes a detection PMT component, a darkroom shutter, a receiving light lens, an excitation light source, an excitation light lens, a dichroic mirror, a lens group, a darkroom box, a darkroom box cover, and a darkroom box cover fixing plate. The excitation light source, the excitation lens, the dichroic mirror, the receiving lens, the darkroom shutter, and the lens are assembled on the darkroom cover plate. The detection PMT assembly is installed at the slot of the darkroom. The darkroom cover plate is connected to the darkroom via the darkroom cover plate fixing plate.

4. The detection device based on photo-induced chemiluminescence as described in claim 1, characterized in that, The incubation mechanism includes an incubation tray, a heating belt, a temperature sensor, an over-temperature protection switch, an incubation tray support column, and insulation cotton. The incubation tray has multiple trays arranged in an arc shape. The incubation tray is used to load reagent cartridges and incubate them at a constant temperature via thermal conduction. The heating belt is located on the side of the incubation tray to enclose it. The temperature sensor is electrically connected to the incubation tray. The over-temperature protection switch is located on the incubation tray. The incubation tray support column is located on the bottom surface of the incubation tray. The insulation cotton is fitted over the outside of the incubation tray. A discharge trough is located in the center of the incubation tray.

5. The detection device based on photo-induced chemiluminescence as described in claim 1, characterized in that, The mixing mechanism includes a reagent cartridge transfer power source, a mixing fixing seat, a vibration module, a vibration module fixing plate, a card pressing driver, a card pressing pin, a card pressing top plate, and a mixing mechanism fixing bracket. The card pressing pin is connected to the card pressing top plate, and the card pressing top plate is mounted on the card pressing driver. The card pressing driver is mounted on the upper part of the mixing mechanism fixing bracket and is used to press the reagent cartridge and limit movement during the mixing process. The vibration module is located at the lower part of the mixing mechanism fixing bracket. The mixing fixing seat is connected to the reagent cartridge transfer power source through an L-shaped connecting plate. The mixing fixing seat is used to load the reagent cartridge and is located at the lower part of the mixing mechanism fixing bracket. The mixing fixing seat is located on one side of the vibration module.

6. The detection device based on photo-induced chemiluminescence as described in claim 1, characterized in that, The box picking and placing mechanism includes an XY plane rotation drive module, a Z-axis lifting drive module, a box picking and placing gripper, a rotating arm, and a box picking and placing fixed bracket. The XY plane rotation drive module is installed on the upper part of the box picking and placing fixed bracket, the Z-axis lifting drive module is installed on the lower part of the box picking and placing fixed bracket, the box picking and placing gripper is installed on the rotating arm, and the rotating arm is installed on the rotation axis of the box picking and placing fixed bracket. The XY plane rotation drive module is connected to the gear mounted on the rotation shaft via a rack, and the Z-axis lifting drive module is connected to the idler wheel mounted on the drive module mounting bracket via a rack.

7. The detection device based on photo-induced chemiluminescence as described in claim 6, characterized in that, The box picking and placing mechanism also includes a light shield, which is installed on the box picking and placing gripper. The light shield is used by the box picking and placing gripper to pick up the reagent card box and transfer it to the photometric module. During photometric measurement, it forms a dark room environment with the photometric slot to avoid the influence of ambient light on the test results.

8. The detection device based on photo-induced chemiluminescence as described in claim 1, characterized in that, The sample feeding mechanism includes a motor mounting base, a linear motor drive module, a feed trough, a material cup sensor, a first photoelectric sensor, a second photoelectric sensor, a photoelectric sensor baffle, and a photoelectric sensor mounting plate; The feed trough is used to hold reagent card boxes for testing; the motor mounting base is set on the testing seat and connected to the linear motor drive module; the material cup sensor is installed on the back of the feed trough; the feed trough is connected to the slider on the linear motor drive module through a connecting plate; the first photoelectric sensor and the second photoelectric sensor are respectively installed at both ends of the photoelectric sensor mounting plate and located on one side of the linear motor drive module; the photoelectric sensor baffle is installed on the slider of the linear motor drive module and is used to trigger and cooperate with the first photoelectric sensor and the second photoelectric sensor.

9. The detection device based on photo-induced chemiluminescence as described in claim 1, characterized in that, The detection base includes a base plate, side plates, a circuit board mounting plate, a power socket, and a data socket; The side plate is mounted on the base plate, the power socket is mounted on the side plate and used to connect to an external power source, the data socket is mounted on the side plate and used to connect to an external device, and the circuit board fixing plate is connected to the upper part of the side plate. A control host is also installed on the side panel, and a main control board is provided on the circuit board fixing plate.

10. The detection device based on photo-induced chemiluminescence as described in claim 9, characterized in that, The detection seat also includes at least two elastic support feet, which are distributedly installed on the bottom surface of the base plate; The elastic support foot includes a first telescopic rod assembly, a second telescopic rod assembly, an elastic element, and a foot cup. Both the first telescopic rod assembly and the second telescopic rod assembly include a first connecting rod and a second connecting rod that are hinged to each other. The end of the first connecting rod away from the second connecting rod is rotatably connected to the base plate through a first pin. The end of the second connecting rod away from the first connecting rod is rotatably connected to the foot cup through a second pin. The elastic element is connected between the base plate and the foot cup.

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