A droplet generation and cultivation device

Abstract

A kind of droplet generation and culture device, including chip base, chip cover plate, coil pipe, coil pipe base and coil pipe cover plate, it is simple in structure, installation and replacement are convenient, can be flexibly configured as droplet generation chip or droplet sorting chip;Droplet system in coil pipe is stable, coil pipe is easy to disassemble, can meet the needs of droplet offline or online culture respectively, reduce the risk of displacement, fusion, rupture, pollution etc. of droplet in collection and transport process.

Description

Technical Field

[0001] This invention relates to the field of droplet microfluidics, specifically to a droplet generation and cultivation device. Background Technology

[0002] Microfluidics is a technology that manipulates tiny volumes of liquid based on microfluidic chips. In biochemical experiments, these dispersed microdroplet units are used to replace conventional reactors in the laboratory, enabling miniaturized biochemical reactions and detections. Typically, after generating droplets using microfluidics, the droplets need to be cultured in situ or offline, depending on the experimental requirements. The common practice is to collect the generated droplets in simple plastic or glass containers for cultivation or preservation, and then pump the droplets from the containers into the microfluidic instrument for sorting. This method is complex and suffers from problems such as fragility and contamination during droplet transfer. Summary of the Invention

[0003] To address the aforementioned problems, this invention provides a droplet generation and cultivation device, comprising a chip base, a chip cover plate, a coil, a coil base, and a coil cover plate. The chip base has a channel groove and a first positioning post; a channel is placed within the channel groove; a second positioning post is provided on the chip cover plate. The coil base has a first positioning hole and a protrusion; several limiting posts are provided along the outer edge of the coil base, and an annular space formed between the limiting posts and the protrusion is used to place the coil. The coil cover plate has a second positioning hole, and the first positioning post, the second positioning post, the first positioning hole, and the second positioning hole are positioned correspondingly. The channel groove includes a first oil phase channel groove, a water phase channel groove, a gas phase channel groove, a first droplet channel groove, and a waste liquid channel groove, which are cross-connected. The channel groove also includes a second oil phase channel groove, a second droplet channel groove, and a sorting channel groove, which are T-shaped connected.

[0004] Preferably, the coil cover plate is engaged with the coil base.

[0005] Preferably, the chip base and the chip cover are engaged and connected, and small holes are provided at the intersection of the outer edges of the chip base and the chip cover with the channel groove.

[0006] Preferably, a quick-connect fitting is included, through which the coil is connected to the pipe.

[0007] Preferably, a Robert stop clamp is provided on the coil.

[0008] This invention provides a droplet generation and cultivation device with a simple structure, convenient installation and replacement, and can be flexibly configured as a droplet generation chip or a droplet sorting chip. The droplet system inside the coil is stable, and the coil is easy to disassemble. It can meet the needs of offline or online droplet cultivation, and reduce the risks of droplet displacement, fusion, breakage, and contamination during collection and transportation. Attached Figure Description

[0009] The above and / or additional aspects and advantages of the present invention will become apparent and readily understood from the description of the embodiments taken in conjunction with the following drawings, in which:

[0010] Figure 1 This is a schematic diagram of the structure of an embodiment of the present invention;

[0011] Figure 2 This is a schematic diagram of the chip base structure according to an embodiment of the present invention;

[0012] Figure 3 This is a schematic diagram of the chip cover structure according to an embodiment of the present invention;

[0013] Figure 4 This is a schematic diagram of the coil base structure according to an embodiment of the present invention;

[0014] Figure 5 This is a schematic diagram of the coil cover plate structure according to an embodiment of the present invention;

[0015] Figure 6 This is a schematic diagram of the coil according to an embodiment of the present invention;

[0016] Figure 7 This is a schematic diagram of a droplet generation chip structure configured according to an embodiment of the present invention;

[0017] Figure 8 This is a schematic diagram of droplet generation according to an embodiment of the present invention;

[0018] Figure 9 This is a schematic diagram of a droplet sorting chip structure configured according to an embodiment of the present invention;

[0019] Figure 10 This is a schematic diagram of droplet sorting according to an embodiment of the present invention. Detailed Implementation

[0020] Embodiments of the present invention are described in detail below, examples of which are shown in the accompanying drawings, wherein the same or similar reference numerals denote the same or similar elements or elements having the same or similar functions throughout. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain the present invention, and should not be construed as limiting the present invention.

[0021] In the description of this invention, it should be understood that the terms "center," "longitudinal," "lateral," "length," "width," "thickness," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," "clockwise," and "counterclockwise," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this invention.

[0022] In the description of this invention, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "linking" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection, an electrical connection, or a connection that allows for communication; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components. Those skilled in the art can understand the specific meaning of the above terms in this invention according to the specific circumstances.

[0023] The following disclosure provides many different embodiments or examples for implementing different structures of the present invention. To simplify the disclosure of the present invention, the components and arrangements of specific examples are described below. Of course, these are merely examples and are not intended to limit the present invention.

[0024] Please see Figure 1-6 This invention provides a droplet generation and cultivation device 1, including a chip base 11, a chip cover plate 12, a coil 15, a coil base 13, and a coil cover plate 14. The chip base 11 has a channel groove 112 and a first positioning post 111. The channel groove 112 is used to house the channel. The chip cover plate 12 has a second positioning post 121. The coil base 13 has a first positioning hole 133 and a protrusion 131. A plurality of limiting posts 132 are arranged along the outer edge of the coil base 13 in a direction perpendicular to the plane of the coil base 13. The annular space formed between the limiting posts 132 and the protrusion 131 is used to place the coil 15. The coil cover plate 14 has a second positioning hole 141. The positions of the first positioning post 111, the second positioning post 121, the first positioning hole 133, and the second positioning hole 141 correspond to each other.

[0025] The droplet generation and cultivation device 1 is positioned by the first positioning post 111, the second positioning post 121, the first positioning hole 133 and the second positioning hole 141, and the chip base 11, the chip cover plate 12, the coil base 13 and the coil cover plate 14 are stacked and assembled from bottom to top in sequence.

[0026] Pipeline 112 includes a first oil phase pipeline 114, a water phase pipeline 115, a gas phase pipeline 116, a first droplet pipeline 117, and a waste liquid pipeline 118. The first oil phase pipeline 114, water phase pipeline 115, gas phase pipeline 116, and first droplet pipeline 117 are cross-connected. Pipeline 112 also includes a second oil phase pipeline 119, a second droplet pipeline 120, and a sorting pipeline 121. The second oil phase pipeline 119, the second droplet pipeline 120, and the sorting pipeline 121 are T-shaped connected.

[0027] like Figure 7 As shown, in some embodiments, the chip base 11 can be configured as a droplet generating chip. The first oil phase pipeline 1141, the water phase pipeline 1151, the gas phase pipeline 1161, and the first droplet pipeline 1171 are respectively provided in the first oil phase pipeline 114, the water phase pipeline 115, the gas phase pipeline 116, and the first droplet pipeline 117, and their connection outlets are connected by cross joints. The waste liquid pipeline 1181 is provided in the waste liquid pipeline 118, and the two ends of the coil 15 are respectively connected to the first droplet pipeline 1171 and the waste liquid pipeline 1181.

[0028] like Figure 8 As shown, in this mode, oil phase, water phase and gas phase are input through the first oil phase pipeline 1141, water phase pipeline 1151 and gas phase pipeline 1161 respectively, continuously generating water phase-gas phase-oil phase interleaved droplets and entering the coil 15. The uneven droplets generated in the previous stage can be discharged through the waste liquid pipeline 1181.

[0029] like Figure 9 As shown, in some embodiments, the chip base 11 can be configured as a droplet sorting chip. The first oil phase channel 1141, the gas phase channel 116, the first droplet channel 117, the second oil phase channel 119, the second droplet channel 120, and the sorting channel 121 are respectively built into the first oil phase channel 1141, the gas phase channel 1161, the first droplet channel 1171, the second oil phase channel 1191, the second droplet channel 1201, and the sorting channel 1211. The first oil phase channel 1141, the gas phase channel 1161, and the first droplet channel 1171 are connected in a T-shape, and the second oil phase channel 1191, the second droplet channel 1201, and the sorting channel 1211 are connected in a T-shape. The two ends of the coil 15 are respectively connected to the first droplet channel 1171 and the second droplet channel 1201.

[0030] participate Figure 10 In this mode, droplets with oil-gas phase spacing are continuously generated by the first oil phase pipeline 1141 and the gas phase pipeline 1161, which drive the droplets in the coil to move into the second droplet pipeline 1201. At the same time, the second oil phase pipeline 1191 also continuously inputs oil phase to increase the spacing between droplets, which is convenient for downstream detection and sorting.

[0031] The coil cover 14 is preferably engaged with the coil base 13, and the chip base 11 is preferably engaged with the chip cover 12. This connection method is simple in structure and easy to use.

[0032] Furthermore, several small holes 113 are made at the intersection of the chip base 11 and the chip cover 12 with the channel groove to facilitate the passage of the channel.

[0033] In some embodiments, the coil 15 is connected to the pipe via a quick-connect fitting 152, enabling quick insertion and removal; Robert stop clamps 151 are provided at both ends of the coil 15 to control the flow rate. After a certain number of droplets are generated, the Robert stop clamps 151 are clamped to effectively seal the coil 15, ensuring that the droplets inside the coil 15 are in a static state and preventing the droplets from flowing.

[0034] Furthermore, if it is necessary to remove the coil for offline culture, clamp the Robert stop clamp 151, disconnect the quick connector 152, and remove the coil.

Claims

1. A droplet generation and cultivation device, characterized in that, It includes a chip base, a chip cover, a coil, a coil base, and a coil cover. The chip base is provided with a pipe groove and a first positioning post. A pipe is placed in the pipe groove. A second positioning post is provided on the chip cover. The coil base is provided with a first positioning hole and a protrusion. Several limiting posts are provided on the outer edge of the coil base. The annular space formed by the limiting posts and the protrusion is used to place the coil. The coil cover is provided with a second positioning hole. The positions of the first positioning post, the second positioning post, the first positioning hole and the second positioning hole are corresponding. The pipeline includes a first oil phase pipeline, a water phase pipeline, a gas phase pipeline, a first droplet pipeline, and a waste liquid pipeline, and the first oil phase pipeline, water phase pipeline, gas phase pipeline, and first droplet pipeline are cross-connected. The pipeline also includes a second oil phase pipeline, a second droplet pipeline, and a sorting pipeline, which are connected in a T-shape.

2. The droplet generation and cultivation device according to claim 1, characterized in that, The coil cover plate is engaged with the coil base.

3. The droplet generation and cultivation device according to claim 1, characterized in that, The chip base and the chip cover are engaged and connected, and small holes are provided at the intersection of the outer edges of the chip base and the chip cover with the channel groove.

4. The droplet generation and cultivation device according to claim 1, characterized in that, Includes a quick-connect fitting, through which the coil is connected to the pipe.

5. The droplet generation and cultivation device according to claim 1, characterized in that, Robert's water-stop clamp is installed on the coil.

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