Production system for preparing 5-aminolevulinic acid hydrochloride by using 5-aminolevulinic acid fermentation liquor

Abstract

The utility model discloses a production system for preparing 5-aminolevulinic acid hydrochloride by using 5-aminolevulinic acid fermentation liquor, and relates to the technical field of preparation of 5-aminolevulinic acid hydrochloride, a 5-aminolevulinic acid fermentation liquor tank is communicated with a ceramic membrane device, the ceramic membrane device is communicated with a first mixing and separating tank, and the first mixing and separating tank is communicated with a second mixing and separating tank. An inlet of the first mixing and separating tank is communicated with a mixed solvent tank, an outlet of the first mixing and separating tank is communicated with a first organic phase tank, and an outlet of the first organic phase tank is communicated to the second mixing and separating tank; an inlet of the second mixing and separating tank is communicated with a hydrochloric acid solution tank, an outlet of the second mixing and separating tank is communicated with a second water phase tank, an outlet of the second water phase tank is communicated to a concentration and crystallization tank, an inlet of the concentration and crystallization tank is respectively communicated with a seed crystal tank and an ethanol tank, and an outlet of the concentration and crystallization tank is communicated with a finished product tank through a pipeline. The whole process does not generate sewage which is difficult to treat, the method is environment-friendly, the steps are simple, and the product is high in yield and good in quality.

Description

Technical Field

[0001] The utility model relates to the technical field of 5-aminolevulinic acid hydrochloride preparation, and in particular to a production system for preparing 5-aminolevulinic acid hydrochloride by utilizing 5-aminolevulinic acid fermentation liquid. Background Art

[0002] 5-Aminolevulinic acid (5-ALA) is a non-protein amino acid that is widely present in living organisms. It is an essential precursor for the biosynthesis of tetrapyrrole compounds such as heme, chlorophyll, and vitamin B12, and is also an intermediate raw material for the preparation of 5-aminolevulinic acid hydrochloride.

[0003] Chinese patent CN111517973B discloses a production method for preparing 5-aminolevulinic acid hydrochloride from a fermentation broth, wherein a crude 5-aminolevulinic acid hydrochloride is prepared from a fermentation broth containing 5-aminolevulinic acid, and then the crude 5-aminolevulinic acid hydrochloride is re-dissolved and crystallized with hydrochloric acid to obtain 5-aminolevulinic acid hydrochloride. The process is highly practical, environmentally friendly, and has low equipment cost. However, ammonia water is required for elution during the reaction process, and the alkalinity of ammonia water will cause certain damage to the product, reducing the quality of the product. At the same time, the ammonia nitrogen wastewater generated has a large environmental pollution and is difficult to handle. Summary of the invention

[0004] The technical problem to be solved by the utility model is: in view of the deficiencies in the prior art, a production system for preparing 5-aminolevulinic acid hydrochloride by utilizing 5-aminolevulinic acid fermentation liquid is provided, and the product has high quality and is environmentally friendly.

[0005] In order to solve the above technical problems, the technical solution of the utility model is:

[0006] A production system for preparing 5-aminolevulinic acid hydrochloride using 5-aminolevulinic acid fermentation liquid, comprising a 5-aminolevulinic acid fermentation liquid tank, the outlet of the 5-aminolevulinic acid fermentation liquid tank is connected to a ceramic membrane device through a pipeline, the clear liquid outlet of the ceramic membrane device is connected to a first mixing and separation tank through a pipeline, the inlet of the first mixing and separation tank is connected to a mixed solvent tank through a pipeline, the outlet of the first mixing and separation tank is connected to a first organic phase tank and a first aqueous phase tank through a pipeline, and the outlet of the first organic phase tank is connected to a second mixing and separation tank through a pipeline;

[0007] The inlet of the second mixing and separation tank is connected to the hydrochloric acid solution tank through a pipeline, the outlet of the second mixing and separation tank is connected to the second organic phase tank and the second aqueous phase tank through a pipeline, the outlet of the second aqueous phase tank is connected to the concentration and crystallization tank through a pipeline, the inlet of the concentration and crystallization tank is connected to the seed tank and the ethanol tank through pipelines, respectively, and the outlet of the concentration and crystallization tank is connected to the finished product tank through a pipeline.

[0008] Preferably, the clear liquid outlet of the ceramic membrane device is connected to an ultrafiltration membrane device through a pipeline, and the clear liquid outlet of the ultrafiltration membrane device is connected to the first mixing and separation tank through a pipeline.

[0009] Preferably, the filtration pore size of the ceramic membrane device is 50-200 nm.

[0010] Preferably, the filtration pore size of the ultrafiltration membrane device is 1000-10000Da.

[0011] Preferably, the outlet of the second water phase tank is connected to an activated carbon filter through a pipeline, the outlet of the activated carbon filter is connected to a decolorizing liquid tank through a pipeline, and the outlet of the decolorizing liquid tank is connected to the concentration and crystallization tank through a pipeline.

[0012] Preferably, the outlet of the first organic phase tank is provided with a first material transfer pump, and the outlet of the first material transfer pump is connected to the first mixing and separation tank and the second mixing and separation tank through pipelines, respectively.

[0013] Preferably, a second material transfer pump is provided at the outlet of the decolorizing liquid tank, and the outlet of the second material transfer pump is connected to the second mixing and separation tank and the concentration and crystallization tank respectively through pipelines.

[0014] Preferably, the outlet of the second organic phase tank is connected to the inlet of the mixed solvent tank through a pipeline.

[0015] Preferably, the outlet of the concentration crystallization tank is connected to a dryer through a pipeline, and the outlet of the dryer is connected to the finished product tank through a pipeline.

[0016] Due to the adoption of the above technical solution, the beneficial effects of the utility model are:

[0017] The utility model discloses a production system for preparing 5-aminolevulinic acid hydrochloride by utilizing 5-aminolevulinic acid fermentation liquid, comprising a 5-aminolevulinic acid fermentation liquid tank, an outlet of the 5-aminolevulinic acid fermentation liquid tank is connected with a ceramic membrane device through a pipeline, a clear liquid outlet of the ceramic membrane device is connected with a first mixing and separation tank through a pipeline, an inlet of the first mixing and separation tank is connected with a mixed solvent tank through a pipeline, an outlet of the first mixing and separation tank is connected with a first organic phase tank and a first aqueous phase tank through a pipeline, and an outlet of the first organic phase tank is connected to a second mixing and separation tank through a pipeline; an inlet of the second mixing and separation tank is connected with a hydrochloric acid solution tank through a pipeline, an outlet of the second mixing and separation tank is connected with a second organic phase tank and a second aqueous phase tank through a pipeline, an outlet of the second aqueous phase tank is connected to a concentration and crystallization tank through a pipeline, an inlet of the concentration and crystallization tank is respectively connected with a seed tank and an ethanol tank through pipelines, and an outlet of the concentration and crystallization tank is connected with a finished product tank through a pipeline. 5-aminolevulinic acid fermentation liquid first enters the ceramic membrane device, and the ceramic membrane clear liquid obtained after removing the fermentation bacteria enters the first mixing and separation tank, and extracts and stands for separation between the mixed solvent composed of dinonylnaphthalenesulfonic acid, tributyl phosphate and ethyl acetate, 5-aminolevulinic acid is dissolved in the mixed solvent and enters the first organic phase tank, and then the liquid in the first organic phase tank enters the second mixing and separation tank again, mixes with the hydrochloric acid solution, and 5-aminolevulinic acid re-enters the aqueous phase, and then stands for separation and temporarily stores in the second aqueous phase tank. The material in the second aqueous phase tank enters the concentration crystallization tank, adds crystal seeds for crystal cultivation, and adds ethanol, and 5-aminolevulinic acid hydrochloride crystals are precipitated by cooling and crystallizing to obtain 5-aminolevulinic acid hydrochloride finished product. The whole process does not produce difficult-to-treat sewage, is environmentally friendly, and has simple steps, and has a high yield and good quality of the product.

[0018] The clear liquid outlet of the ceramic membrane device is connected to the ultrafiltration membrane device through a pipeline, and the clear liquid outlet of the ultrafiltration membrane device is connected to the first mixing and separation tank through a pipeline. The ultrafiltration membrane device removes proteins and macromolecular organic matter in the feed liquid, further improving the purity of the product.

[0019] The filtration pore size of the ceramic membrane device is 50-200nm, and the filtration pore size of the ultrafiltration membrane device is 1000-10000Da, which has a good filtering effect on the feed liquid and improves the purity of the product.

[0020] The outlet of the second water phase tank is connected to an activated carbon filter through a pipeline, the outlet of the activated carbon filter is connected to a decolorizing liquid tank through a pipeline, and the outlet of the decolorizing liquid tank is connected to the concentration crystallization tank through a pipeline. The slurry is decolorized by the activated carbon filter, the chromaticity of the product is improved, and the obtained 5-aminolevulinic acid hydrochloride product has high quality.

[0021] The outlet of the first organic phase tank is provided with a first material transfer pump, and the outlet of the first material transfer pump is connected to the first mixing and separation tank and the second mixing and separation tank through pipelines. The first material transfer pump is used to circulate the first mixing and separation tank externally, so that the materials in the first mixing and separation tank are mixed evenly, the extraction effect is good, the extraction rate is high, and the yield of the product is improved.

[0022] The outlet of the decolorizing liquid tank is provided with a second material transfer pump, and the outlet of the second material transfer pump is connected to the second mixing and separation tank and the concentration and crystallization tank through pipelines. By repeatedly stripping the decolorizing liquid, the yield of the product is further improved, and the waste of raw materials is greatly avoided.

[0023] The outlet of the second organic phase tank is connected to the inlet of the mixed solvent tank through a pipeline. The organic phase obtained after stripping can be directly returned to the first mixing and separation tank to extract 5-aminolevulinic acid, thereby improving the utilization rate of raw materials and reducing the cost of raw materials.

[0024] The outlet of the concentration crystallization tank is connected to a dryer through a pipeline, and the outlet of the dryer is connected to the finished product tank through a pipeline. By drying the product, the purity and quality of the product are further improved. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] The utility model is further described below in conjunction with the accompanying drawings and embodiments.

[0026] Figure 1 It is a schematic diagram of the structure of an embodiment of the utility model;

[0027] Wherein: 1. 5-aminolevulinic acid fermentation liquid tank; 2. ceramic membrane device; 3. first mixing and separation tank; 4. mixed solvent tank; 5. first organic phase tank; 6. first aqueous phase tank; 7. second mixing and separation tank; 8. hydrochloric acid solution tank; 9. second organic phase tank; 10. second aqueous phase tank; 11. concentration and crystallization tank; 12. seed tank; 13. ethanol tank; 14. finished product tank; 15. ultrafiltration membrane device; 16. activated carbon filter; 17. decolorization liquid tank; 18. first material transfer pump; 19. second material transfer pump; 20. dryer. DETAILED DESCRIPTION

[0028] The present invention is further described below in conjunction with the accompanying drawings and embodiments.

[0029] like Figure 1As shown, a production system for preparing 5-aminolevulinic acid hydrochloride by using 5-aminolevulinic acid fermentation liquid comprises a 5-aminolevulinic acid fermentation liquid tank 1, the outlet of the 5-aminolevulinic acid fermentation liquid tank 1 is connected to a ceramic membrane device 2 through a pipeline, the clear liquid outlet of the ceramic membrane device 2 is connected to a first mixing and separation tank 3 through a pipeline, the inlet of the first mixing and separation tank 3 is connected to a mixed solvent tank 4 through a pipeline, the outlet of the first mixing and separation tank 3 is connected to a first organic phase tank 5 and a first aqueous phase tank 6 through a pipeline, the first aqueous phase tank 6 directly discharges sewage, and the outlet of the first organic phase tank 5 is connected to a second mixing and separation tank 7 through a pipeline; the inlet of the second mixing and separation tank 7 is connected to a hydrochloric acid solution tank 8 through a pipeline, and the outlet of the second mixing and separation tank 7 is connected to a second organic phase tank 9 and a second Aqueous phase tank 10, the outlet of the second aqueous phase tank 10 is connected to the concentration crystallization tank 11 through a pipeline, the inlet of the concentration crystallization tank 11 is respectively connected to the seed tank 12 and the ethanol tank 13 through pipelines, and the outlet of the concentration crystallization tank 11 is connected to the finished product tank 14 through a pipeline. The 5-aminolevulinic acid fermentation liquid first enters the ceramic membrane device 2, and the ceramic membrane clear liquid obtained after removing the fermentation bacteria enters the first mixing and separation tank 3, and is extracted with the mixed solvent composed of dinonylnaphthalenesulfonic acid, tributyl phosphate and ethyl acetate and is allowed to stand for separation. 5-aminolevulinic acid is dissolved in the mixed solvent and enters the first organic phase tank 5, and then the liquid in the first organic phase tank 5 enters the second mixing and separation tank 7 again, mixes with the hydrochloric acid solution, and the 5-aminolevulinic acid re-enters the aqueous phase, and then is temporarily stored in the second aqueous phase tank 10 after standing for separation. The material in the second water phase tank 10 enters the concentration crystallization tank 11, and seed crystals are added for crystal cultivation, and ethanol is added to precipitate 5-aminolevulinic acid hydrochloride crystals by cooling and crystallizing to obtain 5-aminolevulinic acid hydrochloride finished product. The whole process does not produce difficult-to-treat sewage, is environmentally friendly, has simple steps, and has high yield and good quality of the product.

[0030] The clear liquid outlet of the ceramic membrane device 2 is connected to the ultrafiltration membrane device 15 through a pipeline, and the clear liquid outlet of the ultrafiltration membrane device 15 is connected to the first mixing and separation tank 3 through a pipeline. The ultrafiltration membrane device 15 removes proteins and macromolecular organic matter in the feed liquid, further improving the purity of the product.

[0031] The filtration pore size of the ceramic membrane device 2 is 50-200nm, and the filtration pore size of the ultrafiltration membrane device 15 is 1000-10000Da, which has a good filtering effect on the feed liquid and improves the purity of the product.

[0032] The outlet of the second aqueous phase tank 10 is connected to an activated carbon filter 16 through a pipeline, and the outlet of the activated carbon filter 16 is connected to a decolorizing liquid tank 17 through a pipeline, and the outlet of the decolorizing liquid tank 17 is connected to the concentration crystallization tank 11 through a pipeline. The slurry is decolorized by the activated carbon filter 16, the chromaticity of the product is improved, and the obtained 5-aminolevulinic acid hydrochloride product has high quality.

[0033] The outlet of the first organic phase tank 5 is provided with a first material transfer pump 18, and the outlets of the first material transfer pump 18 are respectively connected to the first mixing and separation tank 3 and the second mixing and separation tank 7 through pipelines. The first material transfer pump 18 performs external circulation on the first mixing and separation tank 3, and the materials in the first mixing and separation tank 3 are mixed uniformly, the extraction effect is good, the extraction rate is high, and the yield of the product is improved.

[0034] The outlet of the decolorizing liquid tank 17 is provided with a second transfer pump 19, and the outlet of the second transfer pump 19 is respectively connected to the second mixing and separation tank 7 and the concentration and crystallization tank 11 through pipelines. By repeatedly stripping the decolorizing liquid, the yield of the product is further improved, and the waste of raw materials is greatly avoided.

[0035] The outlet of the second organic phase tank 9 is connected to the inlet of the mixed solvent tank 4 through a pipeline. The organic phase obtained after stripping can be directly returned to the first mixing and separation tank 3 to extract 5-aminolevulinic acid, thereby improving the utilization rate of raw materials and reducing the cost of raw materials.

[0036] The outlet of the concentration crystallization tank 11 is connected to the dryer 20 through a pipeline, and the outlet of the dryer 20 is connected to the finished product tank 14 through a pipeline. By drying the product, the purity and quality of the product are further improved.

[0037] It should be understood that these embodiments are only used to illustrate the present invention and are not used to limit the scope of the present invention. In addition, it should be understood that after reading the content taught by the present invention, those skilled in the art can make various changes or modifications to the present invention, and these equivalent forms also fall within the scope defined by the claims attached to this application.

Claims

1. A production system for preparing 5-aminolevulinic acid hydrochloride using 5-aminolevulinic acid fermentation broth, comprising a 5-aminolevulinic acid fermentation broth tank, Features: The outlet of the 5-aminolevulinic acid fermentation liquid tank is connected to a ceramic membrane device through a pipeline, the clear liquid outlet of the ceramic membrane device is connected to a first mixing and separation tank through a pipeline, the inlet of the first mixing and separation tank is connected to a mixed solvent tank through a pipeline, the outlet of the first mixing and separation tank is connected to a first organic phase tank and a first aqueous phase tank through a pipeline, and the outlet of the first organic phase tank is connected to a second mixing and separation tank through a pipeline; The inlet of the second mixing and separation tank is connected to the hydrochloric acid solution tank through a pipeline, the outlet of the second mixing and separation tank is connected to the second organic phase tank and the second aqueous phase tank through a pipeline, the outlet of the second aqueous phase tank is connected to the concentration and crystallization tank through a pipeline, the inlet of the concentration and crystallization tank is connected to the seed tank and the ethanol tank through pipelines, respectively, and the outlet of the concentration and crystallization tank is connected to the finished product tank through a pipeline.

2. The production system for preparing 5-aminolevulinic acid hydrochloride using 5-aminolevulinic acid fermentation broth as claimed in claim 1, Features: The clear liquid outlet of the ceramic membrane device is connected to the ultrafiltration membrane device through a pipeline, and the clear liquid outlet of the ultrafiltration membrane device is connected to the first mixing and separation tank through a pipeline.

3. The production system for preparing 5-aminolevulinic acid hydrochloride using 5-aminolevulinic acid fermentation broth as claimed in claim 1, Features: The filtration pore size of the ceramic membrane device is 50-200nm.

4. The production system for preparing 5-aminolevulinic acid hydrochloride using 5-aminolevulinic acid fermentation broth as claimed in claim 2, Features: The filtration pore size of the ultrafiltration membrane device is 1000-10000Da.

5. The production system for preparing 5-aminolevulinic acid hydrochloride using 5-aminolevulinic acid fermentation broth as claimed in claim 1, Features: The outlet of the second water phase tank is connected to an activated carbon filter through a pipeline, the outlet of the activated carbon filter is connected to a decolorizing liquid tank through a pipeline, and the outlet of the decolorizing liquid tank is connected to the concentration crystallization tank through a pipeline.

6. The production system for preparing 5-aminolevulinic acid hydrochloride using 5-aminolevulinic acid fermentation broth as claimed in claim 1, Features: The outlet of the first organic phase tank is provided with a first material transfer pump, and the outlet of the first material transfer pump is connected to the first mixing and separation tank and the second mixing and separation tank through pipelines respectively.

7. The production system for preparing 5-aminolevulinic acid hydrochloride using 5-aminolevulinic acid fermentation broth as claimed in claim 5, Features: The outlet of the decolorizing liquid tank is provided with a second material transfer pump, and the outlet of the second material transfer pump is connected to the second mixing and separation tank and the concentration and crystallization tank through pipelines respectively.

8. The production system for preparing 5-aminolevulinic acid hydrochloride by using 5-aminolevulinic acid fermentation broth as claimed in claim 1, Features: The outlet of the second organic phase tank is connected to the inlet of the mixed solvent tank through a pipeline.

9. The production system for preparing 5-aminolevulinic acid hydrochloride by using 5-aminolevulinic acid fermentation broth as claimed in claim 1, Features: The outlet of the concentration crystallization tank is connected to a dryer through a pipeline, and the outlet of the dryer is connected to the finished product tank through a pipeline.

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