Reaction assembly of microgravity microorganism reaction device
By designing the spherical reaction chamber and through-type oxygen supply membrane tube, the problem of difficulty in contacting the culture medium in the prior art is solved, and a larger area of oxygen contact with the culture medium is achieved, and the oxygen supply efficiency of the microgravity microbial reactor is improved. and experimental feasibility.
Patent Information
- Application Number
- CN202323663203.2
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2023-12-29
- Publication Date
- 2024-12-10
- Estimated Expiration
- 2033-12-29
AI Technical Summary
When existing microgravity bioreactors cultivate aerobic microorganisms, the gas is difficult to come into contact with the culture medium on a large scale due to the box structure, resulting in insufficient oxygen supply.
设计一种微重力微生物反应装置的反应组件，包括球体反应仓、贯穿式供氧膜管和换液供气组件，供氧膜管贯穿球体反应仓的中心轴线，增加氧气与培养基的接触区域，并通过监控组件和远端计算机控制系统优化气体和液体的供应和排放。
It effectively increases the contact area between gas and culture medium, improves the oxygen supply of aerobic microorganisms, and improves the reliability of experimental data and the feasibility of experimental conditions.
Smart Images
Figure CN222139126U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of microbial research, in particular to a reaction component of a microgravity microbial reaction device. Background Art
[0002] With the rapid development of aerospace technology in recent years, human beings are moving forward in their exploration of deep space. At the same time, the demand for more in-depth research at the cellular and molecular biology levels on the various biological effects and physiological processes of living organisms in space environments (especially weightlessness / microgravity environments) is also increasing. However, the reality is that the technical conditions of spacecraft, space shuttles and space stations are limited, the cost of carrying cell biological samples is high, and astronauts are usually required to cooperate in manual operations in the middle of the journey to obtain relatively ideal experimental data, so it is difficult to implement and the feasibility is not high.
[0003] Microgravity cell culture technology is a cell culture technology carried out in a microgravity environment. This technology simulates the microgravity environment on Earth through special equipment, including the use of reciprocating rollers, liquid agitators, rotary incubators and other devices. For example, the Chinese invention patent application with the public number CN116904305A discloses a liquid-changing microgravity bioreactor, and the Chinese invention patent application with the public number CN112675929A discloses an integrated machine and method for providing a simulated microgravity effect for organisms. However, the microgravity bioreactor of the prior art has the following problems when used: when using the reactor to cultivate aerobic microorganisms, because the box body is a cube as a whole and only the oxygen supply assembly is arranged on the inner periphery of the box body, when the reactor rotates 360 degrees, the gas always moves upward, making it difficult for the gas to contact the culture medium over a large area, and ultimately causing the problem of insufficient oxygen supply to aerobic microorganisms. Therefore, how to increase the contact area between the gas and the culture medium in the reactor is an urgent problem to be solved. Summary of the invention
[0004] The utility model aims to solve one of the above-mentioned technical problems and provides a reaction component of a microgravity microbial reaction device, which can increase the contact area between the gas and the culture medium in the reactor.
[0005] In order to achieve the above-mentioned purpose, the technical solution adopted by the utility model is:
[0006] A reaction component of a microgravity microbial reaction device comprises a mounting and fixing frame, a spherical reaction chamber, an oxygen supply membrane tube, a liquid replacement and air supply component and a monitoring component. The spherical reaction chamber is detachably mounted in the mounting and fixing frame. The oxygen supply membrane tube is installed in the spherical reaction chamber and runs through the central axis of the spherical reaction chamber. The liquid replacement and air supply component is used to realize the functions of supplying oxygen, replacing liquid and exhausting gas to the spherical reaction chamber. The liquid replacement and air supply component is connected to the oxygen supply membrane tube, and the monitoring component is connected to the liquid replacement and air supply component.
[0007] Furthermore, the oxygen supply membrane tube includes a through tube and a plurality of ring tubes, the through tube passes through the opposite ends of the spherical reaction chamber along the central axis of the spherical reaction chamber, the plurality of ring tubes are arranged on the inner wall of the spherical reaction chamber and are arranged at intervals along the circumference of the spherical reaction chamber, and the opposite ends of the plurality of ring tubes are connected to the opposite ends of the through tube; the liquid exchange and air supply assembly is connected to the through tube.
[0008] Furthermore, the reaction component of the microgravity microbial reaction device also includes a cleaning piece, which includes a cleaning ring and a telescopic rod. The cleaning ring is sleeved on the outside of the through-tube and fits against the outer wall of the through-tube. The telescopic rod penetrates the spherical reaction chamber and is equipped with one end of the through-tube, and one end of the telescopic rod is connected to the cleaning ring.
[0009] Furthermore, the cleaning piece also includes a spring, which is sleeved on the outside of the telescopic rod, one end of the spring is connected to the inner wall of the spherical reaction chamber, and the other end of the spring is connected to the cleaning ring.
[0010] Furthermore, the fluid exchange and air supply component includes an air intake system, an exhaust system, a liquid inlet system, a liquid drainage system and a temperature control system; the air intake system and the exhaust system are connected to the oxygen supply membrane tube; the liquid inlet system and the liquid drainage system are fixedly connected to the spherical reaction chamber; the monitoring component is electrically connected to the air intake system, the exhaust system, the liquid inlet system, the liquid drainage system and the temperature control system; the monitoring component is communicatively connected to a remote computer; the remote computer is equipped with a neural convolutional network to receive data from the monitoring component and control the operation of the air intake system, the exhaust system, the liquid inlet system, the liquid drainage system and the temperature control system.
[0011] Furthermore, a micro Ph value detector is also provided on the spherical reaction chamber, and the micro Ph value detector is electrically connected to the monitoring component.
[0012] Due to the adoption of the above technical solution, the utility model has the following beneficial effects:
[0013] When using the reaction components of the above-mentioned microgravity microbial reaction device, since the oxygen supply membrane tube runs through the spherical reaction chamber, when oxygen enters the culture medium through the oxygen supply membrane tube and moves upward, the distance and area that the oxygen passes from the central axis position of the spherical reaction chamber to the inner wall of the spherical reaction chamber are greater than those of the reaction box in the prior art, thereby increasing the contact area between the gas and the culture medium in the reactor. BRIEF DESCRIPTION OF THE DRAWINGS
[0014] Figure 1 This is a schematic diagram of the installation structure of the reaction components of the microgravity microbial reaction device.
[0015] Figure 2 for Figure 1 sectional view of .
[0016] Figure 3 for Figure 2 Enlarged view at point A in the figure.
[0017] In the accompanying drawings, 1-installation fixing frame, 2-spherical reaction chamber, 3-oxygen supply membrane tube, 31-through tube, 32-ring tube, 41-cleaning ring, 42-telescopic rod, 43-spring, 5-micro Ph value detector, 611-gas supply bottle, 612-pressure reducing valve, 613-gas flow meter, 614-waste gas chamber, 615-one-way exhaust valve, 616-liquid supply bottle group, 617-waste liquid collection chamber, 618-liquid outlet peristaltic pump, 619-heating jacket. DETAILED DESCRIPTION
[0018] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.
[0019] It should be noted that when a component is referred to as being "fixed to" another component, it may be directly on the other component or there may also be a component centered. When a component is considered to be "connected to" another component, it may be directly connected to the other component or there may also be a component centered. When a component is considered to be "set on" another component, it may be directly set on the other component or there may also be a component centered. The terms "vertical", "horizontal", "left", "right" and similar expressions used herein are for illustrative purposes only.
[0020] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as those commonly understood by those skilled in the art in the technical field of the present invention. The terms used herein in the specification of the present invention are only for the purpose of describing specific embodiments and are not intended to limit the present invention. The term "and / or" used herein includes any and all combinations of one or more related listed items.
[0021] like Figures 1 to 3 As shown, a preferred embodiment of the utility model provides a reaction component of a microgravity microbial reaction device, including an installation and fixing frame 1, a spherical reaction chamber 2, an oxygen supply membrane tube 3, a liquid replacement and air supply component and a monitoring component. The spherical reaction chamber 2 is detachably installed in the installation and fixing frame 1, the oxygen supply membrane tube 3 is installed in the spherical reaction chamber 2 and runs through the central axis of the spherical reaction chamber 2, the liquid replacement and air supply component is used to realize the functions of supplying oxygen, replacing liquid and exhausting gas to the spherical reaction chamber 2, the liquid replacement and air supply component is connected to the oxygen supply membrane tube 3, and the monitoring component is connected to the liquid replacement and air supply component.
[0022] When using the reaction components of the above-mentioned microgravity microbial reaction device, since the oxygen supply membrane tube 3 is arranged to penetrate the spherical reaction chamber 2, when oxygen enters the culture medium through the oxygen supply membrane tube 3 and moves upward, the distance and area that the oxygen passes from the central axis position of the spherical reaction chamber 2 to the inner wall of the spherical reaction chamber 2 are greater than those of the reaction box in the prior art, thereby increasing the contact area between the gas and the culture medium in the reactor.
[0023] In this embodiment, the oxygen supply membrane tube 3 includes a through tube 31 and a plurality of ring tubes 32. The through tube 31 penetrates the opposite ends of the spherical reaction chamber 2 along the central axis of the spherical reaction chamber 2, and the plurality of ring tubes 32 are arranged on the inner wall of the spherical reaction chamber 2 and are arranged at intervals along the circumference of the spherical reaction chamber 2, and the opposite ends of the plurality of ring tubes 32 are connected with the opposite ends of the through tube 31; the liquid replacement and gas supply assembly is connected to the through tube 31. The through tube 31 and the ring tube 32 are both polytetrafluoroethylene membrane tubes in the prior art, specifically hydrophobic membranes. When oxygen is supplied to the spherical reaction chamber 2, oxygen can enter the culture medium from the through tube 31 and the ring tube 32. The oxygen supplied through the through tube 31 can start from the central axis position of the spherical reaction chamber 2 to the inner wall of the spherical reaction chamber 2. The oxygen supplied through the ring tube 32 can move upward from the inner wall of the spherical reaction chamber 2, thereby increasing the area where oxygen moves in the spherical reaction chamber 2.
[0024] In this embodiment, the reaction assembly of the microgravity microbial reaction device also includes a cleaning piece, and the cleaning piece includes a cleaning ring 41 and a telescopic rod 42. The cleaning ring 41 is sleeved on the outside of the through tube 31 and fits with the outer wall of the through tube 31. The telescopic rod 42 penetrates the spherical reaction chamber 2 and is equipped with one end of the through tube 31, and one end of the telescopic rod 42 is connected to the cleaning ring 41. The telescopic rod 42 adopts a micro-electric telescopic rod in the prior art, and the telescopic rod 42 is electrically connected to the monitoring component. Since the through tube 31 is in the culture medium for a long time, the metabolites of the microorganisms will adhere to the through tube 31. When the telescopic rod 42 is extended or shortened, the cleaning ring 41 is driven to move back and forth along the length direction of the through tube 31 to clean the left and right outer walls of the through tube 31, effectively avoiding the blockage of the oxygen supply holes caused by the metabolites adhering to the outer wall of the through tube 31.
[0025] In this embodiment, the cleaning member further includes a spring 43, which is sleeved on the outside of the telescopic rod 42, one end of the spring 43 is connected to the inner wall of the spherical reaction chamber 2, and the other end of the spring 43 is connected to the cleaning ring 41. The arrangement of the spring 43 prevents the cleaning ring 41 from moving too fast and affecting the growth environment of the microorganisms in the culture medium.
[0026] In this embodiment, the liquid replacement and gas supply assembly includes an air intake system, an exhaust system, a liquid intake system, a liquid discharge system and a temperature control system. The air intake system and the exhaust system are connected to the oxygen supply membrane tube 3. Specifically, the air intake system includes the gas supply bottle 611, which is detachably connected to one end of the mounting and fixing frame 1. The top of the gas supply bottle 611 is connected to one end of the spherical reaction chamber 2 through a gas supply connecting pipe. The gas supply connecting pipe is provided with a pressure reducing valve 612 and a gas flow meter 613 in sequence. The pressure reducing valve 612 and the gas flow meter 613 are electrically connected to the monitoring assembly respectively. The inner wall of the gas supply bottle 611 is fixedly connected to the through-tube 31, and the gas supply connecting pipe is fixedly connected to the through-tube 31.
[0027] The exhaust system includes an exhaust gas bin 614, which is detachably connected to the top of the fixed frame 1. The bottom end of the exhaust gas bin 614 is fixedly connected to the top of the through pipe 31 through an exhaust gas pipe. A one-way exhaust valve 615 is provided on the exhaust gas pipe, and the one-way exhaust valve 615 is electrically connected to the monitoring component.
[0028] The liquid inlet system and the liquid discharge system are fixedly connected to the spheroid reaction chamber 2. Specifically, the liquid inlet system includes a liquid supply bottle group 616, which is detachably connected to the bottom of the mounting frame 1. The liquid supply bottle group 616 is fixedly connected to the spheroid reaction chamber 2 through a liquid inlet peristaltic pump. The liquid supply bottle group 616 is used to supply acid, alkali and nutrient solution to the spheroid reaction chamber 2. The liquid discharge system includes a waste liquid collection chamber 617, which is detachably connected to the mounting frame 1. The bottom of the waste liquid collection chamber 617 is fixedly connected to the top of the mounting frame 1 through a waste liquid pipe. The waste liquid pipe is provided with a liquid outlet peristaltic pump 618, and the liquid outlet peristaltic pump 618 is electrically connected to the monitoring component. The liquid discharge system is set to discharge the culture waste liquid in the spheroid reaction chamber 2 at a regular time.
[0029] The temperature control system includes a heating sleeve 619 and a micro temperature detector. The heating sleeve 619 is sleeved on the circumference of the spherical reaction chamber 2. The micro temperature detector is installed on the spherical reaction chamber 2. The heating sleeve 619 and the micro temperature detector are both electrically connected to the monitoring component. The heating sleeve 619 is used to heat the spherical reaction chamber 2 to maintain a constant temperature reaction environment inside the spherical reaction chamber 2. The micro temperature detector is used to detect the actual temperature of the spherical reaction chamber 2.
[0030] The spherical reaction chamber 2 is also provided with a micro Ph value detector 5, and the micro Ph value detector 5 is electrically connected to the monitoring component.
[0031] The monitoring component is communicatively connected to a remote computer, and a neural convolution network is mounted in the remote computer to receive data from the monitoring component and control the operation of the telescopic rod 42, the air intake system, the exhaust system, the liquid intake system, the liquid discharge system, the temperature control system and the micro Ph value detector 5. The operation and working principle of the monitoring component controlling the air intake system, the exhaust system, the liquid intake system, the liquid discharge system, the temperature control system and the micro Ph value detector 5 are all prior art, and specific reference may be made to the control method and principle of the core control element for the air intake system, the exhaust system, the liquid intake system, the liquid discharge system, the temperature control system and the micro Ph value detector in the Chinese invention patent application with the public number CN116904305A.
[0032] It can be understood that in other embodiments, the spring 43 can be omitted.
[0033] The above description is a detailed description of the preferred feasible embodiment of the utility model, but the embodiment is not used to limit the scope of the patent application of the utility model. All equivalent changes or modified changes completed under the technical spirit suggested by the utility model should fall within the patent scope covered by the utility model.
Claims
1. A reaction assembly of a microgravity microbial reaction device, characterized in that: The invention comprises an installation and fixing frame (1), a spherical reaction chamber (2), an oxygen supply membrane tube (3), a liquid replacement and air supply component and a monitoring component. The spherical reaction chamber (2) is detachably installed in the installation and fixing frame (1). The oxygen supply membrane tube (3) is installed in the spherical reaction chamber (2) and runs through the central axis of the spherical reaction chamber (2). The liquid replacement and air supply component is used to realize the functions of supplying oxygen, replacing liquid and exhausting gas to the spherical reaction chamber (2). The liquid replacement and air supply component is connected to the oxygen supply membrane tube (3), and the monitoring component is connected to the liquid replacement and air supply component.
2. The reaction assembly of a microgravity microbial reaction device according to claim 1, characterized in that: The oxygen supply membrane tube (3) comprises a through tube (31) and a plurality of ring tubes (32); the through tube (31) penetrates the opposite ends of the spherical reaction chamber (2) along the central axis of the spherical reaction chamber (2); the plurality of ring tubes (32) are arranged on the inner wall of the spherical reaction chamber (2) and are arranged at intervals along the circumference of the spherical reaction chamber (2); the opposite ends of the plurality of ring tubes (32) are both connected to the opposite ends of the through tube (31); the liquid exchange and gas supply assembly is connected to the through tube (31).
3. The reaction assembly of a microgravity microbial reaction device according to claim 2, characterized in that: The reaction assembly of the microgravity microbial reaction device also includes a cleaning piece, which includes a cleaning ring (41) and a telescopic rod (42). The cleaning ring (41) is sleeved on the outside of the through-tube (31) and fits with the outer wall of the through-tube (31). The telescopic rod (42) penetrates the spherical reaction chamber (2) and is equipped with one end of the through-tube (31), and one end of the telescopic rod (42) is connected to the cleaning ring (41).
4. The reaction assembly of a microgravity microbial reaction device according to claim 3, characterized in that: The cleaning member further comprises a spring (43), wherein the spring (43) is sleeved on the outside of the telescopic rod (42), one end of the spring (43) is connected to the inner wall of the spherical reaction chamber (2), and the other end of the spring (43) is connected to the cleaning ring (41).
5. The reaction assembly of a microgravity microbial reaction device according to claim 1, characterized in that: The liquid replacement and air supply component comprises an air intake system, an exhaust system, a liquid intake system, a liquid discharge system and a temperature control system; the air intake system and the exhaust system are connected to the oxygen supply membrane tube (3); the liquid intake system and the liquid discharge system are fixedly connected to the spherical reaction chamber (2); the monitoring component is electrically connected to the air intake system, the exhaust system, the liquid intake system, the liquid discharge system and the temperature control system; the monitoring component is communicatively connected to a remote computer; the remote computer is equipped with a neural convolutional network for receiving data from the monitoring component and controlling the operation of the air intake system, the exhaust system, the liquid intake system, the liquid discharge system and the temperature control system.
6. The reaction assembly of a microgravity microbial reaction device according to claim 5, characterized in that: The spherical reaction chamber (2) is also provided with a micro Ph value detector (5), and the micro Ph value detector (5) is electrically connected to the monitoring component.