Sterile subpackaging device for lentiviral vectors

Abstract

The utility model relates to the technical field of lentiviral vector sterile subpackaging, in particular to a lentiviral vector sterile subpackaging device which comprises a supporting box body, a material changing groove is formed in the outer side face of the supporting box body, and a sealing groove is formed in the top of the supporting box body. The sterile subpackaging device for the lentiviral vector comprises a supporting box body, a transparent sealing cover is arranged in the supporting box body, two control keys are installed at the corner of one end of the top of the outer side face of the supporting box body, and a vacuum pipe is connected to the top of the side face of one end of the supporting box body. Meanwhile, the ultraviolet disinfection lamp is used for further sterilizing and disinfecting the internal vacuum environment of the transparent sealing cover, and the lentiviral vector is aseptically subpackaged in the internal sterile environment of the transparent sealing cover through the lengthened leather glove, so that the lentiviral vector after subpackaging is effectively prevented from being polluted.

Description

Technical Field

[0001] The utility model relates to the technical field of aseptic subpackaging of lentiviral vectors, in particular to an aseptic subpackaging device for lentiviral vectors. Background Art

[0002] Lentivirus is a type of viral vector modified from human immunodeficiency virus (HIV). It is a type of retrovirus. Its genome is RNA, and its toxic genes have been deleted and replaced by exogenous target genes. This vector has the characteristics of infecting both dividing and non-dividing cells, and can effectively integrate exogenous genes or exogenous shRNA into host chromosomes, thereby achieving persistent expression.

[0003] When aseptically packaging lentiviral vectors, the general operating method is to sterilize the lentiviral vectors before packaging. However, the packaging process is exposed to the air and the packaging bottles are not sterilized, so the lentiviral vectors are easily carried with bacteria in the air after packaging.

[0004] In summary, the utility model solves the existing problems by designing a lentiviral vector aseptic packaging device. Utility Model Content

[0005] In order to solve the problems raised in the above background technology, the utility model provides a lentiviral vector aseptic packaging device, which has the advantages of being able to ensure that the lentiviral vector can be packaged in a sterile environment by vacuuming, ultraviolet disinfection and filtering with a sterilizing filter.

[0006] In order to achieve the above purpose, the utility model provides the following technical solutions:

[0007] A lentiviral vector aseptic packaging device comprises a supporting box, wherein a material exchange groove is provided on the outer side surface of the supporting box, a sealing groove is provided on the top of the supporting box, two control buttons are installed at the corner of one end of the top of the outer side surface of the supporting box, and a vacuum tube is connected to the top of one end of the side surface of the supporting box;

[0008] A transparent sealing cover is installed on the top of the supporting box body, an operating ring is installed on the bottom of the outer side of the transparent sealing cover, the inner side of the operating ring is located inside the transparent sealing cover and is connected to an extended leather glove, and ultraviolet disinfection lamps are symmetrically installed on both sides of the inside of the transparent sealing cover;

[0009] A support plate is installed at the inner bottom of the transparent sealing cover, electric push rods are installed at the four corners of the top of the support plate, a sterilizing filter is installed at one end of the top of the support plate, and a classification groove is opened at a corner of the top of the support plate.

[0010] As a preferred solution of the utility model, the inner side of the material exchange groove is the same as the inner side of the sealing groove, and the bottom of the sealing groove is in contact with the top connecting edge of the support plate. A sealing strip is provided on the top connecting edge of the support plate, which is in contact with the bottom of the sealing groove to form a seal.

[0011] As a preferred solution of the utility model, a vacuum machine is installed inside one end of the supporting box body, and the exhaust port of the vacuum machine is sealedly connected to one end of the vacuum tube on the outer side of the supporting box body, and one end of the vacuum tube is connected to the top center of the transparent sealing cover and communicates with the interior of the transparent sealing cover.

[0012] As a preferred solution of the utility model, the control button is provided with two for respectively controlling the lifting and lowering operations of the electric push rod, and when the electric push rod is raised, it drives the support plate to form a seal with the sealing groove, and when the electric push rod is lowered, the support plate can be moved to the inner bottom of the material changing groove.

[0013] As a preferred solution of the utility model, the transparent sealing cover is made of transparent tempered glass, and the two ultraviolet disinfection lamps inside the transparent sealing cover are automatically turned on after the electric push rod is raised.

[0014] As a preferred solution of the utility model, the extended leather gloves are sealed to the inner edge of the operating ring, and the designed length of the extended leather gloves can meet the needs of operations in various directions on the support plate.

[0015] Compared with the prior art, the beneficial effects of the utility model are:

[0016] In the utility model, a lentiviral vector aseptic packaging device is designed, a vacuum tube is used to evacuate the interior of a transparent closed cover, and an ultraviolet disinfection lamp is used to further sterilize and disinfect the internal vacuum environment of the transparent closed cover. In addition, the lentiviral vector is aseptically packaged in the aseptic environment inside the transparent closed cover by using extended leather gloves, which effectively prevents the lentiviral vector from easily carrying bacteria in the air after packaging. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 It is a schematic diagram of the overall structure of the utility model;

[0018] Figure 2 This is a schematic diagram of the support box structure of the utility model;

[0019] Figure 3 This is an expanded view of the internal structure of the transparent sealing cover of the utility model;

[0020] Figure 4 This is a schematic diagram of the top structure of the support plate of the utility model.

[0021] In the figure: 1. Support box; 101. Material changing slot; 102. Sealing slot; 2. Control button; 3. Vacuum tube; 4. Transparent sealing cover; 5. Operating ring; 6. Extended leather gloves; 7. Ultraviolet disinfection lamp; 8. Support plate; 9. Electric push rod; 10. Sterilization filter; 11. Classification slot. DETAILED DESCRIPTION

[0022] The technical solutions in the embodiments of the utility model will be clearly and completely described below in conjunction with the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, rather than all of the embodiments. Based on the embodiments of the utility model, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the utility model.

[0023] To facilitate the understanding of the present invention, the present invention will be described more comprehensively below with reference to the relevant drawings, and several embodiments of the present invention are given. However, the present invention can be implemented in many different forms and is not limited to the embodiments described herein. On the contrary, the purpose of providing these embodiments is to make the disclosure of the present invention more thorough and comprehensive.

[0024] It should be noted that when an element is referred to as being "fixed on" another element, it may be directly on the other element or there may also be a central element. When an element is considered to be "connected to" another element, it may be directly connected to the other element or there may be a central element at the same time. The terms "vertical", "horizontal", "left", "right" and similar expressions used in this article are for illustrative purposes only.

[0025] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by technicians in the technical field of the present invention. The terms used in the specification of the present invention are only for the purpose of describing specific embodiments and are not intended to limit the present invention. The term "and / or" used in this article includes any and all combinations of one or more related listed items.

[0026] For examples, see Figure 1-4 , the utility model provides a technical solution:

[0027] A lentiviral vector aseptic packaging device comprises a supporting box 1, a material exchange groove 101 is provided on the outer surface of the supporting box 1, a sealing groove 102 is provided on the top of the supporting box 1, two control buttons 2 are installed at the corner of the top end of the outer side surface of the supporting box 1, and a vacuum tube 3 is connected to the top of one end of the side surface of the supporting box 1;

[0028] When the electric push rod 9 rises, it drives the support plate 8 to form a seal with the sealing groove 102. Since a vacuum machine is installed inside one end of the supporting box 1, and the exhaust port of the vacuum machine is sealed and connected to one end of the vacuum tube 3 on the outer surface of the supporting box 1, the inside of the transparent sealing cover 4 is evacuated by the vacuum machine. Since the survival rate of bacteria in a vacuum is low, the sterilization effect is achieved. At the same time, when the electric push rod 9 is lowered, the support plate 8 can be moved to the inner bottom of the material changing groove 101, and the operating instruments on the support plate 8 can be conveniently replaced through the material changing groove 101. Example

[0029] Based on the embodiment 1, the difference is that a transparent sealing cover 4 is installed on the top of the supporting box 1, an operating ring 5 is installed on the bottom of the outer side of the transparent sealing cover 4, the inner side of the operating ring 5 is located inside the transparent sealing cover 4 and is connected to an extended leather glove 6, and ultraviolet disinfection lamps 7 are symmetrically installed on both sides of the inside of the transparent sealing cover 4;

[0030] The two ultraviolet disinfection lamps 7 inside the transparent sealing cover 4 are automatically turned on after the electric push rod 9 is raised. At this time, the interior of the transparent sealing cover 4 is evacuated and disinfected by ultraviolet light to achieve a sterile operating environment. In the process, the inner side of the extended leather gloves 6 is also disinfected, so that the aseptic packaging operation of the lentiviral vector can be performed in a sterile environment. Example

[0031] Based on Example 1, the difference is that a support plate 8 is installed at the inner bottom of the transparent sealing cover 4, electric push rods 9 are installed at the four corners of the top of the support plate 8, a sterilizing filter 10 is installed at one end of the top of the support plate 8, and a classification groove 11 is opened at a corner of the top of the support plate 8;

[0032] The sterilizing filter 10 can filter and sterilize the lentiviral vector in a sterile environment, further ensuring that the subpackaging of the lentiviral vector is aseptically operated. At the same time, the classification slot 11 can be used to classify and place the subpackaging containers of the lentiviral vector, which is convenient for subsequent sorting.

[0033] The working process of the utility model: using a lentiviral vector aseptic packaging device designed by the present invention, when packaging the lentiviral vector, firstly, when the electric push rod 9 is lowered, the support plate 8 can be moved to the inner bottom of the material replacement tank 101, at this time, the lentiviral vector can be easily placed on the support plate 8, and when the electric push rod 9 is raised, the support plate 8 is driven to form a seal with the sealing groove 102, at this time, the inside of the transparent sealing cover 4 is evacuated by a vacuum machine, because the survival rate of bacteria in a vacuum is low, so the effect of sterilization is achieved, and the two ultraviolet disinfection lamps 7 inside the transparent sealing cover 4 After the electric push rod 9 is raised, it automatically opens. At this time, the interior of the transparent sealing cover 4 is evacuated and disinfected with ultraviolet light to achieve a sterile operating environment. In the process, the inner side of the extended leather gloves 6 is also disinfected, so that the aseptic subpackaging operation of the lentiviral vector can be performed in a sterile environment. In addition, the sterilizing filter 10 can filter and sterilize the lentiviral vector in a sterile environment, further ensuring that the subpackaging of the lentiviral vector reaches a sterile operation. At the same time, the classification slot 11 can be used to classify and place the subpackaging containers of the lentiviral vector, which is convenient for subsequent sorting.

[0034] In summary, the device can ensure that the lentiviral vector can be packaged in a sterile environment by vacuuming, ultraviolet disinfection and sterilization filter filtration, and effectively prevent the lentiviral vector from easily carrying bacteria in the air after packaging.

[0035] Although the embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A lentiviral vector aseptic packaging device, comprising a supporting box (1), Features: A material replacement groove (101) is provided on the outer side surface of the support box (1), a sealing groove (102) is provided on the top of the support box (1), two control buttons (2) are installed at a corner of the top end of the outer side surface of the support box (1), and a vacuum tube (3) is connected to the top of one end of the side surface of the support box (1); A transparent sealing cover (4) is installed on the top of the supporting box (1), an operating ring (5) is installed on the bottom of the outer side of the transparent sealing cover (4), an inner side of the operating ring (5) is located inside the transparent sealing cover (4) and is connected to an extended leather glove (6), and ultraviolet disinfection lamps (7) are symmetrically installed on both sides of the interior of the transparent sealing cover (4); A support plate (8) is installed at the inner bottom of the transparent sealing cover (4), electric push rods (9) are installed at the four corners of the top of the support plate (8), a sterilizing filter (10) is installed at one end of the top of the support plate (8), and a classification groove (11) is opened at a corner of the top of the support plate (8).

2. A lentiviral vector aseptic packaging device according to claim 1, Features: The inner side of the material exchange groove (101) is the same as the inner side of the sealing groove (102), and the bottom of the sealing groove (102) is in contact with the top connecting edge of the support plate (8). A sealing strip is provided on the top connecting edge of the support plate (8) and is in contact with the bottom of the sealing groove (102) to form a seal.

3. A lentiviral vector aseptic packaging device according to claim 1, Features: A vacuum machine is installed inside one end of the support box (1), and the air outlet of the vacuum machine is sealedly connected to one end of a vacuum tube (3) on the outer surface of the support box (1), and one end of the vacuum tube (3) is connected to the top center of the transparent sealing cover (4) and communicates with the interior of the transparent sealing cover (4).

4. A lentiviral vector aseptic packaging device according to claim 1, Features: The control button (2) is provided with two buttons for respectively controlling the lifting and lowering operations of the electric push rod (9), and when the electric push rod (9) is lifted, it drives the support plate (8) to form a seal with the sealing groove (102), and when the electric push rod (9) is lowered, the support plate (8) can be moved to the inner bottom of the material replacement groove (101).

5. A lentiviral vector aseptic packaging device according to claim 1, Features: The transparent sealing cover (4) is made of transparent tempered glass, and the two ultraviolet disinfection lamps (7) inside the transparent sealing cover (4) are automatically turned on after the electric push rod (9) is raised.

6. A lentiviral vector aseptic packaging device according to claim 1, Features: The extended leather gloves (6) are sealed to the inner edge of the operating ring (5), and the designed length of the extended leather gloves (6) can satisfy operations in various directions on the support plate (8).

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