Magnetic aeration stirring device and bioreactor with same

Abstract

The utility model discloses a magnetic aeration stirring device, which comprises a stirring disc circumferentially rotating around the axis of the stirring disc, the stirring disc comprises a paddle assembly and a magnetic rotor assembly, and the rotation of the stirring disc is realized through the magnetic coupling effect between the magnetic rotor assembly and an external driving assembly; the annular aeration disc is arranged on the outer side of the stirring disc in a surrounding mode, the annular aeration disc and the stirring disc are arranged in a split mode, and an air outlet part is arranged on the annular aeration disc; the connecting film is respectively fixed with the stirring disc and the annular aeration disc, so that the stirring disc is hermetically connected with the annular aeration disc through the connecting film. According to the technical scheme, the annular aeration disc and the stirring disc are separately arranged, so that the annular aeration disc and the stirring disc can be independently manufactured, the manufacturing cost is reduced, parts of various models can be conveniently assembled and matched, and the suitability is improved.

Description

Technical Field

[0001] The utility model relates to the technical field of bioreactors, in particular to a magnetic aeration stirring device and a bioreactor with the same. Background Art

[0002] In the production process of biopharmaceuticals, in order to achieve commercial mass production of biological products such as enzymes, antibodies or antibiotics, animal, plant or microbial cells are usually placed in a bioreactor for culture. The interior of the bioreactor is generally equipped with a magnetic aeration and stirring device, which includes a stirring device and an aeration device. The aeration device supplies gases such as O2 and CO2 required by the cells to the interior of the bioreactor. At the same time, the gas supplied by the aeration device is mixed with the feed liquid inside the bioreactor through the stirring device and diffused into the interior of the bioreactor for cell culture; at the same time, the magnetic aeration and stirring device also includes a magnetic rotor device, which can be magnetically coupled with a magnetic drive device outside the bioreactor to drive the magnetic aeration and stirring device to work (the working principle of the magnetic stirring paddle is the prior art and will not be repeated in the present utility model).

[0003] The existing aeration device and stirring device are usually arranged on the same substrate, the stirring device is located in the central position of the substrate, and the aeration device is arranged around the stirring device. However, the above existing structure has the following defects: First, the aeration device and the stirring device are located on the same substrate, which makes the manufacturing process complicated and the manufacturing cost increases accordingly. Secondly, when it is necessary to match to bioreactors of different models and specifications, there are different requirements for the combination of the aeration device and the stirring device, and there is a problem that the aeration device and the stirring device need to be redesigned and manufactured, which prolongs the production cycle and manufacturing cost. Utility Model Content

[0004] In order to overcome the problems in the prior art, the utility model sets the annular aeration disk and the stirring disk separately and manufactures them separately, thereby reducing the manufacturing difficulty and cost; and the independent annular aeration disk and stirring disk can meet the configuration requirements of bioreactors of different models and improve adaptability.

[0005] In order to achieve the above purpose, the utility model adopts the following technical solutions:

[0006] A magnetic aeration stirring device comprises a stirring disk that rotates circumferentially around its own axis, the stirring disk comprises a blade assembly and a magnetic rotor assembly, and the rotation of the stirring disk is achieved through the magnetic coupling between the magnetic rotor assembly and an external driving assembly; and further comprises:

[0007] The annular aeration plate is arranged around the outside of the stirring plate and is separated from the stirring plate. The annular aeration plate is provided with an air outlet;

[0008] The connecting membrane is respectively fixed to the stirring disk and the annular aeration disk, so that the stirring disk and the annular aeration disk are sealed and connected through the connecting membrane.

[0009] In the above technical solution, the separate setting of the annular aeration disk and the stirring disk allows them to be manufactured separately, reducing the manufacturing cost, and the independent annular aeration disk and stirring disk can meet the configuration requirements of bioreactors of different models, improve adaptability, further reduce costs, and be suitable for large-scale production and use. The stirring disk and the annular aeration disk are sealed and connected by a connecting membrane, which can effectively avoid leakage at the connection position between the stirring disk and the annular aeration disk, and ensure the sealing effect of the stirring device.

[0010] Preferably, a gap is provided between the annular aeration disk and the stirring disk, and the gap is continuously arranged around the circumference of the stirring disk, and the connecting membrane is arranged at the gap position.

[0011] In the above technical scheme, in order to meet the needs of different stirring devices, a plurality of annular aeration plates of different sizes and a plurality of stirring plates of different sizes are usually designed. By setting the gap, annular aeration plates and stirring plates with certain differences in size can also be combined and matched without interference, which further improves the adaptability between components of different models of the device, and improves the adaptability and cost-effectiveness of the device.

[0012] Preferably, the radial width of the gap is L1, the axial height of the gap is H1, and the ratio of L1 to H1 is in the range of 0.2-2.

[0013] In the above technical solution, by setting the ratio range of the radial width L1 and the axial height H1 of the gap to be limited within a specific range, the stability of the overall structure after connection and the limiting effect of the annular aeration disc on the stirring disc can be ensured at the same time. This design takes into account the following two aspects: 1. Stability: The larger the radial width L1 of the gap, the lower the stability of the overall structure after connection. This is because during the stirring process, there will be certain vibrations and swings between the stirring disc and the annular aeration disc. Excessive radial width may cause the connection to become loose or the structure to be unstable. By limiting the ratio range of L1 to H1 to a specific range, the stability of the overall structure after connection can be ensured. 2. Adaptability: The larger the axial height H1, the easier it is for the stirring disc to be blocked and limited by the annular aeration disc when it swings left and right relative to the annular aeration disc. Therefore, the limiting effect of the annular aeration disc on the stirring disc in the radial direction is better, that is, stirring discs and annular aeration discs of different models and sizes can be well adapted in the device. By adjusting the ratio range of L1 to H1, stirring discs and annular aeration discs of different radial sizes can be adapted, thereby improving the adaptability and flexibility of the device.

[0014] Preferably, the connecting membrane is an annular closed structure, and the connecting membrane closes the gap.

[0015] In the above technical solution, the connecting membrane of the annular closed structure can adapt to and completely close the annular gap to avoid material accumulation in the gap.

[0016] Preferably, the stirring disc is provided with a positioning portion, and the connecting film is arranged around the outside of the positioning portion.

[0017] In the above technical solution, when the stirring device is assembled, the positioning portion on the stirring disk can position the connecting film, thereby ensuring that the connecting film is accurately positioned on the stirring disk.

[0018] Preferably, the connecting membrane is tensionedly connected between the stirring disk and the annular aeration disk, so that the stirring disk and the annular aeration disk are relatively fixed.

[0019] In the above technical solution, the connecting membrane is tensioned, and the stirring disk and the annular aeration disk can be tightened from all directions, thereby fixing the stirring disk and the annular aeration disk and reducing the shaking of the stirring disk relative to the annular aeration disk during use.

[0020] Preferably, the connecting membrane includes an upper connecting membrane and a lower connecting membrane, the upper connecting membrane is arranged above the stirring disk and the annular aeration disk, and the lower connecting membrane is arranged below the stirring disk and the annular aeration disk, and the upper connecting membrane and the lower connecting membrane are both tensionedly connected between the stirring disk and the annular aeration disk to relatively fix the stirring disk and the annular aeration disk.

[0021] In the above technical scheme, by setting the upper connecting membrane and the lower connecting membrane, the stirring disk and the annular aeration disk can be fixed above and below, thereby increasing the firmness of the connection of the connecting membrane. Compared with the scheme of setting only one connecting membrane, this scheme will not cause sealing failure even if one of the connecting membranes is damaged, thereby reducing the risk of sealing failure due to loose connection or damage of the connecting membrane.

[0022] Preferably, the upper connecting film and the lower connecting film close the gap to form an isolation cavity.

[0023] In the above technical solution, the gap can be isolated to form an isolation cavity through the sealing effect of the upper connecting membrane and the lower connecting membrane, so as to prevent the biological liquid from entering the gap to form material accumulation.

[0024] Preferably, a deformation support body is provided in the isolation cavity.

[0025] In the above technical solution, the deformable support body is arranged in the isolation chamber, which has the effect of increasing the connection strength between the stirring disk and the annular aeration disk. At the same time, the deformable support body also has a buffering effect, which can absorb and disperse the impact and vibration generated during the stirring process, further improving the stability and reliability of the stirring device.

[0026] Preferably, the stirring disk is provided with a first connecting surface connected to the upper connecting membrane and a second connecting surface connected to the lower connecting membrane, and the annular aeration disk is provided with a third connecting surface connected to the upper connecting membrane and a fourth connecting surface connected to the lower connecting membrane, the height difference between the first connecting surface and the second connecting surface is D1, the height difference between the third connecting surface and the fourth connecting surface is D2, and D1 / D2≠1.

[0027] In the above technical solution, by making the upper connecting membrane and the lower connecting membrane tilted, a component force can be generated in the axial direction, so that the stirring disk and the annular aeration disk can be stably and reliably fixed together. This design can effectively prevent the relative displacement between the stirring disk and the annular aeration disk, and improve the stability and reliability of the equipment. At the same time, when subjected to axial external force, the axial component force generated by the upper connecting membrane and the lower connecting membrane can further enhance the stability and reliability of the equipment, ensuring the normal operation and service life of the equipment.

[0028] Preferably, the upper connecting membrane comprises a first inclined section provided between the stirring disk and the annular aeration disk, and the lower connecting membrane comprises a second inclined section provided between the stirring disk and the annular aeration disk, and the angle between the first inclined section and the second inclined section is in the range of 5-30°.

[0029] In the above technical solution, the larger the angle between the first inclined section and the second inclined section, the larger the force component generated by the upper connecting membrane and the lower connecting membrane in the axial direction, and the better the fixing effect on the stirring disk and the annular aeration disk. If the angle is too small, the force component generated by the connecting membrane in the axial direction is too small, and a good fixing effect cannot be achieved. However, the larger the angle, the more obvious the tearing effect of the axial force component on the connecting membrane. The angle between the first inclined section and the second inclined section is limited to between 5-30°, and the force component generated by the upper connecting membrane and the lower connecting membrane in the axial direction is moderate, which can provide a good fixing effect on the stirring disk and the annular aeration disk, and will not produce excessive tearing effect on the connection position. Therefore, the above solution can ensure the reliability and stability of the connecting membrane in the stirring device.

[0030] Preferably, the stirring disk has an upper limit position, a lower limit position and an intermediate position between the upper limit position and the lower limit position relative to the annular aeration disk, so that the stirring disk can move between the upper limit position and the lower limit position along the axial direction of the stirring disk relative to the annular aeration disk.

[0031] In the above technical solution, the stirring disk can move between the upper limit position and the lower limit position along the axial direction of the stirring disk relative to the annular aeration disk, so that the stirring disk can move up and down within a certain range relative to the annular aeration disk, thereby changing the distance between the blade assembly and the gas outlet. This change in distance will lead to different bubble stirring and breaking effects, thereby meeting the specific gas requirements of different culture modes and improving stirring efficiency and quality. Therefore, this design makes the stirring device more flexible and adaptable, and can be adjusted and optimized according to different process requirements.

[0032] Preferably, when the stirring disk is in the middle position, the connecting membrane is in a relaxed state, and when the stirring disk is in the upper limit position or the lower limit position, the connecting membrane is tensioned by the stirring disk and the annular aeration disk.

[0033] In the above technical solution, the connecting film can adapt to the up and down movement of the stirring plate by switching between the tensioned state and the relaxed state. During the up and down movement of the stirring plate, the connecting film can always seal the gap to ensure the sealing effect.

[0034] Preferably, the connecting film is a flexible member.

[0035] In the above technical solution, the flexible connecting film allows the stirring disc to switch smoothly between the upper limit and the lower limit positions, avoiding restrictions on the up and down movement of the stirring disc. When the stirring disc is in the middle position, the flexible connecting film can fold part of its structure to achieve avoidance, so as to adapt to the changes of the stirring disc during the up and down movement. This design utilizes the flexibility and adaptability of the connecting film, ensures the free movement of the stirring disc within the required range, and improves production efficiency and quality.

[0036] Preferably, when the stirring disc is at the upper limit position or the lower limit position, the connecting film is tensioned into a frustum. In the above technical solution, the frustum-shaped connecting film can tighten the stirring disc from multiple directions, and the tensioning force has an axial component and a radial component, so that the stirring disc at the upper limit position or the lower limit position is more stable and reliable.

[0037] Preferably, the connecting membrane is arranged above the gap and closes the upper end surface of the gap; or, the connecting membrane is arranged below the gap and closes the lower end surface of the gap; or, the connecting membrane includes an upper connecting membrane and a lower connecting membrane, the upper connecting membrane is arranged above the stirring disk and the annular aeration disk and closes the upper end surface of the gap, and the lower connecting membrane is arranged below the stirring disk and the annular aeration disk and closes the lower end surface of the gap.

[0038] The above three technical solutions can all seal the gap through the connecting membrane to ensure the sealing effect of the stirring device, and can effectively prevent the liquid inside the bioreactor from leaking through the gap, thereby ensuring the normal operation and production efficiency of the stirring device.

[0039] Preferably, the radial width of the gap is L1, the axial thickness of the connecting film is H2, the axial thickness of a single layer of the connecting film is H2, and the ratio of L1 to H2 is in the range of 2-8.

[0040] In the above technical solution, when the stirring disk moves up and down, the connecting membrane needs to pass through the gap. If the ratio of L1 to H2 is too small, that is, the radial width L1 of the gap is too small relative to the axial thickness H2 of the connecting membrane, the connecting membrane is easy to interfere with the stirring disk and the annular aeration disk in the process of moving and passing through the gap, affecting the movement of the stirring disk and the stability of the connecting membrane. If the ratio of L1 to H2 is too large, that is, the axial thickness H2 of the connecting membrane is too small, its strength is too small, and the radial width of the gap is large, L1 is large, and the required connecting membrane area is large. The connecting membrane with a large area and low strength is more likely to be damaged, which will reduce the reliability and stability of the connection between the stirring disk and the annular aeration disk. Therefore, by reasonably controlling the ratio range of H2 to L1, the stability and reliability of the connecting membrane during the up and down movement of the stirring disk can be ensured, interference can be avoided, and the reliability and stability of the connection between the stirring disk and the annular aeration disk can be ensured.

[0041] Preferably, the connecting film is an elastic member.

[0042] In the above technical solution, the connecting film can adapt to the up and down movement of the stirring plate by elastic expansion and contraction. During the up and down movement of the stirring plate, the connecting film can always seal the gap to ensure the sealing effect. When the stirring plate is in the middle position, the elastic connecting film can achieve avoidance through its own elastic expansion and contraction to adapt to the changes of the stirring plate during the up and down movement. This design utilizes the flexibility and adaptability of the connecting film, ensures the free movement of the stirring plate within the required range, and improves production efficiency and quality.

[0043] Preferably, the axial movement distance of the stirring disc between the upper limit position and the lower limit position is AA, the average axial distance between the blade and the air outlet is BB, and the ratio range of AA to BB is: 0.2-40.

[0044] The above technical solution can adjust the proportional relationship between the moving distance of the stirring plate between the upper and lower limit positions and the average axial distance between the blade and the air outlet by reasonably controlling the ratio range of AA and BB. In this way, the moving range of the stirring plate and the stirring range of the blade can be better matched, thereby optimizing the stirring effect and improving the uniformity and efficiency of stirring.

[0045] Preferably, the stirring disc is provided with an axial positioning surface.

[0046] In the above technical solution, the axial positioning surface is used to position the driving mechanism that drives the stirring disk to rise and fall, so as to ensure that the driving mechanism can accurately drive the stirring disk to rise and fall.

[0047] A bioreactor comprises a flexible bag body and any one of the above-mentioned magnetic aeration and stirring devices, wherein the outer periphery of the annular aeration disk is surrounded by the flexible bag body and sealed and welded.

[0048] In the above technical solution, the annular aeration disk of the magnetic stirring device is surrounded by a flexible bag body and sealed and welded to form a closed internal space of the bioreactor container, so that the external gas enters the internal space of the bioreactor container through the gas outlet, and is fully dispersed by the blade assembly on the stirring disk and then transmitted to the internal space of the bioreactor, thereby realizing effective transmission of the feed liquid in the internal space of the bioreactor.

[0049] The beneficial effects of the above technical solution are:

[0050] 1. The separate setting of the annular aeration disk and the stirring disk allows them to be manufactured separately, reducing the manufacturing cost. The independent annular aeration disk and stirring disk can meet the configuration requirements of different types of bioreactors, improve adaptability, further reduce costs, and are suitable for large-scale production and use. The stirring disk and the annular aeration disk are sealed and connected through a connecting membrane, which can effectively avoid leakage at the connection position between the stirring disk and the annular aeration disk, ensuring the sealing effect of the stirring device.

[0051] 2. By setting the upper connecting membrane and the lower connecting membrane, the stirring disk and the annular aeration disk can be fixed above and below, thereby increasing the firmness of the connection of the connecting membrane. Compared with the solution of setting only one connecting membrane, this solution will not cause sealing failure even if one of the connecting membranes is damaged, thereby reducing the risk of sealing failure due to loose connection or damage of the connecting membrane.

[0052] 3. Through the sealing effect of the upper connecting membrane and the lower connecting membrane, the gap can be isolated to form an isolation cavity, preventing the biological liquid from entering the gap and forming material accumulation.

[0053] 4. The deformable support body is arranged in the isolation chamber, which has the function of increasing the connection strength between the stirring disk and the annular aeration disk. At the same time, the deformable support body also has a buffering effect, which can absorb and disperse the impact and vibration generated during the stirring process, further improving the stability and reliability of the stirring device.

[0054] 5. By making the upper connecting membrane and the lower connecting membrane tilted, a component force can be generated in the axial direction, so that the stirring disc and the annular aeration disc can be stably and reliably fixed together. This design can effectively prevent the relative displacement between the stirring disc and the annular aeration disc, and improve the stability and reliability of the equipment. At the same time, when subjected to axial external force, the axial component force generated by the upper connecting membrane and the lower connecting membrane can further enhance the stability and reliability of the equipment, ensuring the normal operation and service life of the equipment.

[0055] 6. The stirring disc can move between the upper limit position and the lower limit position along the axial direction of the stirring disc relative to the annular aeration disc, so that the stirring disc can move up and down within a certain range relative to the annular aeration disc, thereby changing the distance between the blade assembly and the gas outlet. This change in distance will lead to different bubble stirring and dispersion effects, thereby meeting the specific requirements of different culture modes for gas and improving stirring efficiency and quality. Therefore, this design makes the stirring device more flexible and adaptable, and can be adjusted and optimized according to different process requirements.

[0056] 7. The flexible connecting membrane allows the stirring disc to switch smoothly between the upper and lower limit positions, avoiding restrictions on the up and down movement of the stirring disc. When the stirring disc is in the middle position, the flexible connecting membrane can fold part of its structure to achieve avoidance to adapt to the changes in the stirring disc during the up and down movement. This design utilizes the flexibility and adaptability of the connecting membrane to ensure the free movement of the stirring disc within the required range, thereby improving production efficiency and quality.

[0057] 8. The connecting film can adapt to the up and down movement of the stirring plate through elastic expansion and contraction. During the up and down movement of the stirring plate, the connecting film can always seal the gap to ensure the sealing effect. When the stirring plate is in the middle position, the elastic connecting film can achieve avoidance through its own elastic expansion and contraction to adapt to the changes of the stirring plate during the up and down movement. This design utilizes the flexibility and adaptability of the connecting film, ensures the free movement of the stirring plate within the required range, and improves production efficiency and quality. BRIEF DESCRIPTION OF THE DRAWINGS

[0058] Figure 1 It is a structural schematic diagram of the utility model;

[0059] Figure 2 It is a cross-sectional view of the utility model;

[0060] Figure 3 It is a partial structural schematic diagram of the utility model;

[0061] Figure 4 It is a partial structural section of the utility model Figure 1 ;

[0062] Figure 5yes Figure 4 A partial enlarged view of the middle A;

[0063] Figure 6 It is a partial structural section of the utility model Figure 2 ;

[0064] Figure 7 It is a partial structural section of the utility model Figure 3 ;

[0065] Figure 8 It is a partial structural section of the utility model Figure 4 ;

[0066] Fig. 9 It is a partial structural section of the utility model Figure 5 .

[0067] In the figure: a stirring disc 1, a blade assembly 11, a magnetic rotor assembly 12, a positioning portion 13, a first connecting surface 14, a second connecting surface 15, an axial positioning surface 16, an annular aeration disc 2, an air outlet portion 21, a third connecting surface 22, a fourth connecting surface 23, a connecting membrane 3, an upper connecting membrane 31, a first inclined section 311, a lower connecting membrane 32, a second inclined section 321, a gap 4, an isolation chamber 5, and a deformation support body 6. DETAILED DESCRIPTION

[0068] The present invention will be further described below in conjunction with the accompanying drawings and specific embodiments.

[0069] Embodiment 1:

[0070] like Figures 1 to 7 As shown, a magnetic aeration stirring device comprises a stirring disk 1 which rotates circumferentially around its own axis, an annular aeration disk 2 and a connecting membrane 3. The stirring disk 1 comprises a blade assembly 11 and a magnetic rotor assembly 12. The rotation of the stirring disk 1 is achieved through the magnetic coupling between the magnetic rotor assembly 12 and an external driving assembly. The annular aeration disk 2 is arranged around the outside of the stirring disk 1 and is separated from the stirring disk 1. An air outlet 21 is provided on the annular aeration disk 2. The connecting membrane 3 is respectively fixed to the stirring disk 1 and the annular aeration disk 2, so that the stirring disk 1 and the annular aeration disk 2 are sealed and connected through the connecting membrane 3. The stirring disk 1 is in the shape of a disc, and the annular aeration disk 2 is in the shape of a circular ring. The connecting membrane 3 and the stirring disk 1 are fixed together by ultrasonic welding or thermal welding, and the annular aeration disk 2 and the stirring disk 1 are fixed together by ultrasonic welding or thermal welding.

[0071] In the above technical solution, the separate setting of the annular aeration disk 2 and the stirring disk 1 allows them to be manufactured separately, reducing the manufacturing cost, and facilitating the assembly and matching of various types of components, improving adaptability, further reducing costs, and making it more suitable for large-scale production and use. The stirring disk 1 and the annular aeration disk 2 are sealed and connected through the connecting membrane 3, which can effectively avoid leakage at the connection position and ensure the sealing effect of the stirring device.

[0072] The magnetic aeration stirring device is used for a bioreactor container, and the outer periphery of the annular aeration disk 2 is surrounded by a flexible bag body and sealed and welded to form a closed internal space of the bioreactor container, so that external gas enters the internal space of the bioreactor container through the gas outlet 21. There are multiple gas outlets 21, and the multiple gas outlets 21 are evenly distributed along the axial circumference of the annular aeration disk 2, and each gas outlet 21 is provided with a plurality of gas outlet holes, and the gas outlet 21 is connected to an external gas supply device through a pipeline.

[0073] Specifically, a gap 4 is provided between the annular aeration disk 2 and the stirring disk 1, and the gap 4 is continuously arranged around the circumference of the stirring disk 1, and the connecting membrane 3 is arranged at the position of the gap 4. The connecting membrane 3 is an annular closed structure, and the connecting membrane 3 closes the gap 4. The stirring disk 1 is provided with a positioning portion 13 protruding upward, the side wall of the positioning portion 13 is a cylindrical surface, and the connecting membrane 3 is arranged around the outside of the positioning portion 13.

[0074] In the above technical scheme, in order to meet the needs of different stirring devices, a plurality of annular aeration plates 2 of different sizes and a plurality of stirring plates 1 of different sizes are usually designed. By setting the gap 4, the annular aeration plates 2 and stirring plates 1 of certain size differences can also be assembled and matched without interference, which further improves the adaptability between different types of components of the device, and improves the adaptability and cost-effectiveness of the device. The connecting membrane 3 of the annular closed structure can adapt to and completely close the annular gap 4 to avoid material accumulation in the gap 4. When the stirring device is assembled, the positioning portion 13 on the stirring plate 1 can position the connecting membrane 3 to ensure that the connecting membrane 3 is accurately positioned on the stirring plate 1.

[0075] Preferably, the radial width of the gap 4 is L1, the axial height of the gap 4 is H1, and the ratio of L1 to H1 is in the range of 0.2-2.

[0076] In the above technical solution, by setting the ratio range of the radial width L1 and the axial height H1 of the gap 4 to be limited within a specific range, the stability of the overall structure after connection and the limiting effect of the annular aeration disk 2 on the stirring disk 1 can be ensured at the same time. This design takes the following two aspects into consideration: 1. Stability: The larger the radial width L1 of the gap 4, the lower the stability of the overall structure after connection. This is because during the stirring process, there will be certain vibrations and swings between the stirring disk 1 and the annular aeration disk 2. Excessive radial width may cause the connection to become loose or the structure to be unstable. By limiting the ratio range of L1 to H1 to be within a specific range, the stability of the overall structure after connection can be ensured. 2. Adaptability: The larger the axial height H1 is, the easier it is for the stirring disc 1 to be blocked and limited by the annular aeration disc 2 when it swings left and right relative to the annular aeration disc 2. Therefore, the annular aeration disc 2 has a better limiting effect on the stirring disc 1 in the radial direction. That is, stirring discs 1 and annular aeration discs 2 of different models and sizes can be well adapted in the device. By adjusting the ratio range of L1 to H1, stirring discs 1 and annular aeration discs 2 of different radial sizes can be adapted, thereby improving the adaptability and flexibility of the device.

[0077] Specifically, the material of the connecting film 3 includes one or more of PE, POP, COC, EVOH, PA, PET, PBT, POE, EVA, and EMA. The connecting film 3 can be prepared from a single material or a mixture of multiple materials, and can also be a multilayer film prepared by laminating and compounding multiple materials. An axial positioning surface 16 is provided on the stirring disk 1. The axial positioning surface 16 is used to position the driving mechanism that drives the stirring disk 1 to rise and fall, so as to ensure that the driving mechanism can accurately drive the stirring disk 1 to rise and fall.

[0078] It is understandable that in another embodiment, the inner diameter of the annular aeration disk 2 is the same as the outer diameter of the stirring disk 1, so that the inner wall of the annular aeration disk 2 is in close contact with the outer wall of the stirring disk 1, and the size of the gap is minimized.

[0079] Embodiment 2:

[0080] like Figures 1 to 7As shown, on the basis of Example 1, the connecting membrane 3 is tensionedly connected between the stirring disk 1 and the annular aeration disk 2 so that the stirring disk 1 and the annular aeration disk 2 are relatively fixed. Preferably, the connecting membrane 3 includes an upper connecting membrane 31 and a lower connecting membrane 32, the upper connecting membrane 31 is arranged above the stirring disk 1 and the annular aeration disk 2, and the lower connecting membrane 32 is arranged below the stirring disk 1 and the annular aeration disk 2, and the upper connecting membrane 31 and the lower connecting membrane 32 are both tensionedly connected between the stirring disk 1 and the annular aeration disk 2 so that the stirring disk 1 and the annular aeration disk 2 are relatively fixed. The upper connecting membrane 31 and the lower connecting membrane 32 close the gap 4 to form an isolation chamber 5. A deformation support body 6 is provided in the isolation chamber 5. The material of the deformation support body 6 can be any one of silicone, TPE or PEO or a mixture of several.

[0081] In the above technical solution, by setting the upper connecting membrane 31 and the lower connecting membrane 32, the stirring disk 1 and the annular aeration disk 2 can be fixed above and below, thereby increasing the firmness of the connection of the connecting membrane 3. Compared with the solution of setting only one connecting membrane 3, even if one of the connecting membranes 3 is damaged, the sealing failure will not be caused, thereby reducing the risk of sealing failure caused by loose connection or damage of the connecting membrane 3. In the above technical solution, the gap 4 can be isolated to form an isolation cavity 5 through the sealing effect of the upper connecting membrane 31 and the lower connecting membrane 32, thereby preventing the biological liquid from entering the gap 4 and forming material accumulation. A deformable support body 6 is arranged in the isolation cavity 5, which has the function of increasing the connection strength between the stirring disk 1 and the annular aeration disk 2. At the same time, the deformable support body 6 also has a buffering effect, which can absorb and disperse the impact and vibration generated during the stirring process, further improving the stability and reliability of the stirring device.

[0082] Preferably, Figure 8 As shown, the stirring disc 1 is provided with a first connecting surface 14 connected to the upper connecting membrane 31 and a second connecting surface 15 connected to the lower connecting membrane 32, and the annular aeration disc 2 is provided with a third connecting surface 22 connected to the upper connecting membrane 31 and a fourth connecting surface 23 connected to the lower connecting membrane 32, the height difference between the first connecting surface 14 and the second connecting surface 15 is D1, the height difference between the third connecting surface 22 and the fourth connecting surface 23 is D2, and D1 / D2≠1. The upper connecting membrane 31 includes a first inclined section 311 provided on the stirring disc 1 and the annular aeration disc 2, and the lower connecting membrane 32 includes a second inclined section 321 provided on the stirring disc 1 and the annular aeration disc 2, and the angle α between the first inclined section 311 and the second inclined section 321 is in the range of 5-30°.

[0083] The above technical solution can generate a component force in the axial direction by allowing the upper connecting membrane 31 and the lower connecting membrane 32 to be inclined, so that the stirring disk 1 and the annular aeration disk 2 can be stably and reliably fixed together. This design can effectively prevent the relative displacement between the stirring disk 1 and the annular aeration disk 2, and improve the stability and reliability of the equipment. At the same time, when subjected to axial external force, the axial component force generated by the upper connecting membrane 31 and the lower connecting membrane 32 can further enhance the stability and reliability of the equipment and ensure the normal operation and service life of the equipment. The larger the angle between the first inclined section 311 and the second inclined section 321, the greater the component force generated by the upper connecting membrane 31 and the lower connecting membrane 32 in the axial direction, and the better the fixing effect on the stirring disk 1 and the annular aeration disk 2. If the angle is too small, the connecting membrane 3 will generate too small a component force in the axial direction, and a good fixing effect cannot be achieved. However, the larger the angle, the more obvious the tearing effect of the axial component force on the connecting membrane 3. The angle α between the first inclined section 311 and the second inclined section 321 is in the range of 5-30°, and the force component generated by the upper connecting membrane 31 and the lower connecting membrane 32 in the axial direction is moderate, which can provide a good fixing effect for the stirring disk 1 and the annular aeration disk 2, and will not produce excessive tearing effect on the connection position. Therefore, the above scheme can ensure the reliability and stability of the connecting membrane 3 in the stirring device.

[0084] It can be understood that in another embodiment, D1 = D2. The upper connecting membrane 31 and the lower connecting membrane 32 are both arranged parallel to the radial direction of the annular aeration disk 2.

[0085] It can be understood that in another embodiment, Figure 6 As shown, only one connecting membrane 3 is arranged above the stirring disk 1 and the annular aeration disk 2 .

[0086] It can be understood that in another embodiment, Figure 7 As shown, only one connecting membrane 3 is arranged below the stirring disk 1 and the annular aeration disk 2 .

[0087] Embodiment 3:

[0088] like Figures 1 to 5 and Fig. 9 As shown, on the basis of Example 1, the stirring disc 1 has an upper limit position, a lower limit position and an intermediate position between the upper limit position and the lower limit position relative to the annular aeration disc 2, so that the stirring disc 1 can move between the upper limit position and the lower limit position along the axial direction of the stirring disc 1 relative to the annular aeration disc 2. When the stirring disc 1 is in the intermediate position, the connecting membrane 3 is in a relaxed state, and when the stirring disc 1 is in the upper limit position or the lower limit position, the connecting membrane 3 is tensioned by the stirring disc 1 and the annular aeration disc 2.

[0089] In the above technical solution, the stirring disk 1 can move between the upper limit position and the lower limit position along the axial direction of the stirring disk 1 relative to the annular aeration disk 2, so that the stirring disk 1 can move up and down within a certain range relative to the annular aeration disk 2, thereby changing the distance between the blade assembly 11 and the air outlet 21. This change in distance will lead to different bubble stirring and breaking effects, thereby meeting the specific requirements of different culture modes for gas and improving stirring efficiency and quality. Therefore, this design makes the stirring device more flexible and adaptable, and can be adjusted and optimized according to different process requirements. The connecting membrane 3 can adapt to the up and down movement of the stirring disk 1 by switching between the tensioned state and the relaxed state. During the up and down movement of the stirring disk 1, the connecting membrane 3 can always seal the gap 4 to ensure the sealing effect.

[0090] Preferably, the radial width of the gap 4 is L1, the axial thickness of the single-layer connecting film 3 is H2, and the ratio of L1 to H2 is in the range of 2 to 8. The axial movement distance of the stirring disc 1 between the upper limit position and the lower limit position is AA, the average axial distance between the blade and the air outlet 21 is BB, and the ratio of AA to BB is in the range of 0.2 to 40.

[0091] In the above technical solution, when the stirring disk moves up and down, the connecting membrane 3 needs to pass through the gap 4. If the ratio of L1 to H2 is too small, that is, the radial width L1 of the gap 4 is too small relative to the axial thickness H2 of the connecting membrane 3, the connecting membrane 3 is easy to interfere with the stirring disk 1 and the annular aeration disk 2 in the process of moving and passing through the gap 4, affecting the movement of the stirring disk 1 and the stability of the connecting membrane 3. If the ratio of L1 to H2 is too large, that is, the axial thickness H2 of the connecting membrane 3 is too small, its strength is too small, and the radial width of the gap 4 is large, L1 is large, and the required area of ​​the connecting membrane 3 is large. The connecting membrane 3 with a large area and low strength is more likely to be damaged, which will reduce the reliability and stability of the connection between the stirring disk 1 and the annular aeration disk 2. Therefore, by reasonably controlling the ratio range of L1 to H2, the stability and reliability of the connecting membrane 3 in the process of the stirring disk 1 moving up and down can be ensured, interference can be avoided, and the reliability and stability of the connection between the stirring disk 1 and the annular aeration disk 2 can be ensured. The above technical solution can adjust the proportional relationship between the moving distance of the stirring disc 1 between the upper and lower limit positions and the average axial distance between the blade and the air outlet 21 by reasonably controlling the ratio range of AA and BB. In this way, the moving range of the stirring disc 1 and the stirring range of the blade can be better matched, thereby optimizing the stirring effect and improving the uniformity and efficiency of stirring.

[0092] It is understandable that, in one embodiment, the connecting membrane 3 is disposed above the gap 4 and closes the upper end surface of the gap 4. It is understandable that, in another embodiment, the connecting membrane 3 is disposed below the gap 4 and closes the lower end surface of the gap 4. It is understandable that, in another embodiment, the connecting membrane 3 includes an upper connecting membrane 31 and a lower connecting membrane 32, the upper connecting membrane 31 is disposed above the stirring disk 1 and the annular aeration disk 2 and closes the upper end surface of the gap 4, and the lower connecting membrane 32 is disposed below the stirring disk 1 and the annular aeration disk 2 and closes the lower end surface of the gap 4. The above three technical solutions can all close the gap 4 by the connecting membrane 3 to ensure the sealing effect of the stirring device, and can effectively prevent the liquid inside the bioreactor from leaking through the gap 4, thereby ensuring the normal operation and production efficiency of the stirring device.

[0093] It can be understood that, in one embodiment, the connecting membrane 3 is a flexible member, and the connecting membrane 3 is stretched into a frustum shape. The flexible connecting membrane 3 allows the stirring disk 1 to switch smoothly between the upper limit position and the lower limit position, avoiding restrictions on the up and down movement of the stirring disk 1. When the stirring disk 1 is in the middle position, the flexible connecting membrane 3 can fold part of its own structure to avoid changes in the stirring disk 1 during the up and down movement. This design utilizes the flexibility and adaptability of the connecting membrane 3, ensures the free movement of the stirring disk 1 within the required range, and improves production efficiency and quality. Fig. 9 As shown, when the stirring disk 1 is at the upper limit position or the lower limit position, the frustum-shaped connecting membrane 3 can tighten the stirring disk 1 from multiple directions, and the tensioning force has an axial component and a radial component, so that the stirring disk 1 at the upper limit position or the lower limit position is more stable and reliable.

[0094] It can be understood that in another embodiment, the connecting membrane 3 is an elastic member. The connecting membrane 3 can adapt to the up and down movement of the stirring disk 1 through elastic expansion and contraction. During the up and down movement of the stirring disk 1, the connecting membrane 3 can always seal the gap 4 to ensure the sealing effect. When the stirring disk 1 is in the middle position, the elastic connecting membrane 3 can achieve avoidance through its own elastic expansion and contraction to adapt to the changes of the stirring disk 1 during the up and down movement. This design utilizes the flexibility and adaptability of the connecting membrane 3, ensures the free movement of the stirring disk 1 within the required range, and improves production efficiency and quality. When the stirring disk 1 is in the upper limit position or the lower limit position, the connecting membrane 3 is in a tensioned state, and the tensioned connecting membrane 3 is in a frustum shape. The frustum-shaped connecting membrane 3 can tighten the stirring disk 1 from multiple directions, and the tensioning force has an axial component and a radial component, so that the stirring disk 1 in the upper limit position or the lower limit position is more stable and reliable.

[0095] Embodiment 4:

[0096] A bioreactor comprises a flexible bag body and any one of the above-mentioned magnetic aeration and stirring devices, wherein the outer periphery of the annular aeration disk 2 is surrounded by the flexible bag body and sealed and welded. In this solution, the annular aeration disk 2 of the magnetic stirring device is surrounded by the flexible bag body and sealed and welded to form a closed internal space of the bioreactor, so that the external gas enters the internal space of the bioreactor through the gas outlet, and is fully dispersed by the blade assembly 11 on the stirring disk 1 and then transmitted to the internal space of the bioreactor, thereby realizing the effective transmission of the feed liquid in the internal space of the bioreactor.

Claims

1. A magnetic aeration stirring device, comprising a stirring disk rotating circumferentially around its own axis, the stirring disk comprising a blade assembly and a magnetic rotor assembly, and the rotation of the stirring disk is achieved through the magnetic coupling between the magnetic rotor assembly and an external driving assembly; wherein the stirring disk is further include: The annular aeration plate is arranged around the outside of the stirring plate and is separated from the stirring plate. The annular aeration plate is provided with an air outlet; The connecting membrane is respectively fixed to the stirring disk and the annular aeration disk, so that the stirring disk and the annular aeration disk are sealed and connected through the connecting membrane.

2. A magnetic aeration stirring device according to claim 1, characterized in that: A gap is provided between the annular aeration disk and the stirring disk, and the gap is continuously arranged around the circumference of the stirring disk, and the connecting membrane is arranged at the position of the gap.

3. A magnetic aeration stirring device according to claim 2, characterized in that: The radial width of the gap is L1, the axial height of the gap is H1, and the ratio of L1 to H1 is in the range of 0.2-2.

4. A magnetic aeration stirring device according to claim 2, characterized in that: The connecting film is an annular closed structure, and the connecting film closes the gap.

5. A magnetic aeration stirring device according to claim 4, characterized in that: The stirring disc is provided with a positioning portion, and the connecting film is arranged around the outer side of the positioning portion.

6. A magnetic aeration stirring device according to claim 2, characterized in that: The connecting membrane is tensionedly connected between the stirring disk and the annular aeration disk, so that the stirring disk and the annular aeration disk are relatively fixed.

7. A magnetic aeration stirring device according to claim 6, characterized in that: The connecting membrane includes an upper connecting membrane and a lower connecting membrane, wherein the upper connecting membrane is arranged above the stirring disk and the annular aeration disk, and the lower connecting membrane is arranged below the stirring disk and the annular aeration disk, and the upper connecting membrane and the lower connecting membrane are both tensionedly connected between the stirring disk and the annular aeration disk to relatively fix the stirring disk and the annular aeration disk.

8. A magnetic aeration stirring device according to claim 7, characterized in that: The upper connecting film and the lower connecting film seal the gap to form an isolation cavity.

9. A magnetic aeration stirring device according to claim 8, characterized in that: A deformation support body is arranged in the isolation cavity.

10. The magnetic aeration stirring device according to claim 7, characterized in that: The stirring plate is provided with a first connecting surface connected to the upper connecting membrane and a second connecting surface connected to the lower connecting membrane, the annular aeration plate is provided with a third connecting surface connected to the upper connecting membrane and a fourth connecting surface connected to the lower connecting membrane, the height difference between the first connecting surface and the second connecting surface is D1, the height difference between the third connecting surface and the fourth connecting surface is D2, and D1 / D2≠1.

11. A magnetic aeration stirring device according to claim 10, characterized in that: The upper connecting membrane includes a first inclined section disposed between the stirring disk and the annular aeration disk, and the lower connecting membrane includes a second inclined section disposed between the stirring disk and the annular aeration disk. The angle between the first inclined section and the second inclined section is in the range of 5-30°.

12. The magnetic aeration stirring device according to claim 2, characterized in that: The stirring disc has an upper limit position, a lower limit position and an intermediate position between the upper limit position and the lower limit position relative to the annular aeration disc, so that the stirring disc can move between the upper limit position and the lower limit position along the axial direction of the stirring disc relative to the annular aeration disc.

13. A magnetic aeration stirring device according to claim 12, characterized in that: When the stirring disk is in the middle position, the connecting membrane is in a relaxed state; when the stirring disk is in the upper limit position or the lower limit position, the connecting membrane is in a tensioned state by the stirring disk and the annular aeration disk.

14. The magnetic aeration stirring device according to claim 12, characterized in that: The connecting film is a flexible member.

15. A magnetic aeration stirring device according to claim 14, characterized in that: When the stirring disc is at the upper limit position or the lower limit position, the connecting film is tensioned into a frustum shape.

16. The magnetic aeration stirring device according to claim 12, characterized in that: The connecting membrane is arranged above the gap and closes the upper end surface of the gap; or, the connecting membrane is arranged below the gap and closes the lower end surface of the gap; or, the connecting membrane includes an upper connecting membrane and a lower connecting membrane, the upper connecting membrane is arranged above the stirring disk and the annular aeration disk and closes the upper end surface of the gap, and the lower connecting membrane is arranged below the stirring disk and the annular aeration disk and closes the lower end surface of the gap.

17. A magnetic aeration stirring device according to claim 16, characterized in that: The radial width of the gap is L1, the axial thickness of the single-layer connecting film is H2, and the ratio range of L1 to H2 is: 2-8.

18. The magnetic aeration stirring device according to claim 12, characterized in that: The connecting film is an elastic member.

19. The magnetic aeration stirring device according to claim 12, characterized in that: The axial moving distance of the stirring disc between the upper limit position and the lower limit position is AA, the average axial distance between the blade and the air outlet is BB, and the ratio range of AA to BB is: 0.2-40.

20. The magnetic aeration stirring device according to claim 1, characterized in that: An axial positioning surface is arranged on the stirring disc.

21. A bioreactor, comprising a flexible bag and a magnetic aeration stirring device according to any one of claims 1 to 20, characterized in that: The outer circumference of the annular aeration plate is surrounded by a flexible bag body and sealed and welded.

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