Stem cell exosome preservation device

Abstract

The utility model discloses a stem cell exosome preservation device which comprises a box body, a fixing plate is fixedly connected to the inner wall of the box body, a plurality of electric push rods are fixedly installed at the bottom of the inner side of the box body at equal intervals, the push rod ends of the electric push rods are fixedly connected with a placing base, a placing groove is formed in the top of the placing base, and the placing groove is fixedly connected with the box body. Limiting sleeves are fixedly connected to the tops of the placing seats through fixing rods, test tubes are connected to the limiting sleeves in a penetrating mode, sealing plugs corresponding to the test tubes needing to be taken out are taken down, the designated placing seats, the limiting sleeves and the test tubes are driven by electric push rods to move upwards and penetrate through openings in partition plates, and then the test tubes are taken down. Therefore, the taking operation of the single test tube filled with the stem cell exosome is completed, the situation that all the test tubes need to be exposed in a room temperature state when a certain test tube is taken is further avoided, and the storage effect of the stem cell exosome is further improved.

Description

Technical Field

[0001] The utility model relates to a preservation device, in particular to a stem cell exosome preservation device. Background Art

[0002] Stem cells can secrete exosomes through paracrine effects. Stem cell exosomes have similar biological properties to stem cells, but stem cell exosomes are safer, more stable and efficient, and have stronger and more complex signal molecule transport and regulation capabilities. Exosomes refer to small membrane vesicles 30-150nm containing complex RNA and proteins. Nowadays, they specifically refer to discoid vesicles with a diameter of 40-100nm. Many cells can secrete exosomes under normal and pathological conditions. They are mainly derived from multivesicular bodies formed by the invagination of intracellular lysosomal particles, and are released into the extracellular matrix after the fusion of the multivesicular body membrane with the cell membrane. After being extracted, stem cell exosomes need to be stored in test tubes and stored in a refrigerator.

[0003] Currently, most existing stem cell exosome storage devices use a method of storing and taking out multiple test tubes at the same time. In the process of taking out a test tube, all test tubes need to be exposed to room temperature, which can easily reduce the storage effect of stem cell exosomes. Therefore, a stem cell exosome storage device is proposed to optimize and solve the above problem. Utility Model Content

[0004] The purpose of the utility model is to provide a stem cell exosome storage device to solve the problems raised in the above background technology.

[0005] In order to achieve the above purpose, the utility model provides the following technical solutions:

[0006] A stem cell exosome storage device comprises a box, a battery is fixedly installed at the bottom of the box, a support cover is provided on the outer cover of the battery, a fixing plate is fixedly connected to the inner wall of the box, a plurality of temperature sensors are fixedly embedded at the top of the fixing plate at equal distances, a plurality of electric push rods are fixedly installed at the inner bottom of the box at equal distances, a placement seat is fixedly connected to the push rod ends of the plurality of electric push rods, a placement slot is provided on the top of the placement seat, a limiting sleeve is fixedly connected to the top of the placement seat through a fixing rod, a test tube is penetrated and connected to the limiting sleeve, a fixing frame is fixedly connected to the top of the limiting sleeve through a limiting bolt, a test tube plug is fixedly connected to the inner top of the fixing frame, four heat conducting plates are fixedly connected to the inner walls of the four sides of the box, four semiconductor refrigeration components are penetrated and installed on the four sides of the box, a partition is fixedly connected to the inner wall of the box, a plurality of openings are opened at equal distances and sizes on the partition corresponding to the plurality of fixing frames, a sealing plug is respectively plugged in the plurality of openings, the top of the box is provided with an opening and is covered with a box cover, and a control panel is fixedly installed on the front surface of the box.

[0007] As a further solution of the utility model: heat dissipation mesh holes are symmetrically opened on the box wall, the electric push rod vertically penetrates the fixed plate, one end of the support cover is fixedly connected to the bottom of the box, the fixed plate is opened with a groove for fixing and embedding the temperature sensor, and the fixed plate is opened with a through hole for the electric push rod to penetrate and connect.

[0008] As a further solution of the utility model: the limiting sleeve is provided with a through hole for the test tube to pass through and connect, the bottom end of the test tube fits with the placement groove on the placement seat, the fixing frame and the limiting sleeve are both provided with threaded through holes for the threaded connection of the limiting bolt, and one end of the test tube plug is plugged inside the test tube.

[0009] As a further solution of the utility model: the box body is provided with an opening for the semiconductor refrigeration component to pass through and connect, the cooling side of the semiconductor refrigeration component is fixedly connected to the outer side of the heat conduction plate, the box cover is fixedly connected to the box body by fixing bolts, the box cover and the box body are both provided with threaded through holes for threaded connection of fixing bolts, and the inner top of the box cover fits with the top of the sealing plug.

[0010] As a further solution of the utility model: the top of the box cover is symmetrically connected with a handle, and the control panel is provided with a display screen and control buttons.

[0011] As a further solution of the utility model: the temperature sensor, the electric push rod and the semiconductor refrigeration assembly are electrically connected to the control panel through wires, and the control panel is electrically connected to the battery through wires.

[0012] Compared with the prior art, the beneficial effects of the utility model are:

[0013] By removing the sealing plug of the test tube that needs to be taken out, and using the electric push rod to drive the designated placement seat, the limiting sleeve and the test tube to move up and pass through the opening on the partition, and then removing the test tube, the single test tube containing stem cell exosomes can be taken out, thereby further avoiding the situation where all the test tubes need to be exposed to room temperature when taking one of the test tubes, and further improving the storage effect of the stem cell exosomes. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 Schematic diagram of the structure of the stem cell exosome storage device.

[0015] Figure 2 This is a schematic diagram of the local structure of the stem cell exosome storage device.

[0016] Figure 3 Front view of the stem cell exosome storage device.

[0017] As shown in the figure: box body 1, battery 2, support cover 3, fixing plate 4, temperature sensor 5, electric push rod 6, placement seat 7, limit sleeve 8, test tube 9, fixing frame 10, test tube plug 11, heat conduction plate 12, semiconductor refrigeration component 13, partition 14, sealing plug 15, box cover 16, handle 17 and control panel 18. DETAILED DESCRIPTION

[0018] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0019] See also Figures 1 to 3 In an embodiment of the utility model, a stem cell exosome preservation device includes a box body 1, a battery 2, a support cover 3, a fixing plate 4, a temperature sensor 5, an electric push rod 6, a placement seat 7, a limit sleeve 8, a test tube 9, a fixing frame 10, a test tube plug 11, a heat conducting plate 12, a semiconductor refrigeration component 13, a partition 14, a sealing plug 15, a box cover 16, a handle 17 and a control panel 18. The battery 2 is fixedly installed at the bottom of the box body 1, and the outer side cover of the battery 2 is provided with a support cover 3, one end of the support cover 3 is fixedly connected to the bottom of the box body 1, and the fixing plate 4 is fixedly connected to the inner wall of the box body 1, and a plurality of temperature sensors 5 are fixedly embedded at equal distances on the top of the fixing plate 4.

[0020] The box wall of the box body 1 is symmetrically provided with heat dissipation mesh holes, and a plurality of electric push rods 6 are fixedly installed at equal distances on the bottom inner side of the box body 1, the electric push rods 6 vertically penetrate the fixing plate 4, the fixing plate 4 is provided with a through hole for the electric push rods 6 to penetrate and connect, and the push rod ends of the plurality of electric push rods 6 are fixedly connected with a placement seat 7, the top of the placement seat 7 is provided with a placement groove, the top of the placement seat 7 is fixedly connected with a limiting sleeve 8 through a fixing rod, a test tube 9 is penetrated and connected on the limiting sleeve 8, a through hole for the test tube 9 to penetrate and connect is provided on the limiting sleeve 8, the bottom end of the test tube 9 is fitted with the placement groove on the placement seat 7, the top of the limiting sleeve 8 is fixedly connected with a fixing frame 10 through a limiting bolt, the fixing frame 10 and the limiting sleeve 8 are both provided with a threaded through hole for the threaded connection of the limiting bolt, a test tube plug 11 is fixedly connected to the top of the inner side of the fixing frame 10, and one end of the test tube plug 11 is plugged inside the test tube 9.

[0021] Four heat conducting plates 12 are fixedly connected to the inner walls of the four sides of the box body 1, and four semiconductor refrigeration components 13 are respectively installed through the four side walls of the box body 1. The box body 1 is provided with an opening for the semiconductor refrigeration component 13 to penetrate and connect. The cooling side of the semiconductor refrigeration component 13 is fixedly connected to the outer side of the heat conducting plate 12. A partition 14 is fixedly connected to the inner wall of the box body 1. The partition 14 is provided with multiple openings of equal distance and size corresponding to the multiple fixing frames 10. A sealing plug 15 is respectively plugged in the multiple openings. The top of the box body 1 is provided with an opening and a cover is provided. A box cover 16 is fixedly connected to the box body 1 by fixing bolts. Threaded through holes for threaded connection of the fixing bolts are provided on the box cover 16 and the box body 1. The top of the inner side of the box cover 16 fits with the top of the sealing plug 15. The top of the box cover 16 is symmetrically connected with a handle 17. A control panel 18 is fixedly installed on the front surface of the box body 1. The control panel 18 is provided with a display screen and control buttons. The temperature sensor 5, the electric push rod 6 and the semiconductor refrigeration assembly 13 are electrically connected to the control panel 18 through wires, and the control panel 18 is electrically connected to the battery 2 through wires.

[0022] The model of the temperature sensor 5 is an IRTS infrared temperature sensor, the model of the electric push rod 6 is an HLZ71 DC electric push rod, the model of the semiconductor refrigeration component 13 is a TECA AHP-250CP semiconductor refrigerator, and the model of the control panel 18 is an EMG ECU01 control panel.

[0023] The working principle of the utility model is:

[0024] When the stem cell exosome storage device needs to be used, the personnel first remove the fixing bolts on the box cover 16, and then separate the box cover 16 from the box body 1 by holding the handle 17, and then respectively remove the multiple sealing plugs 15 on the partition 14, and then connect the control panel 18 with the battery 2, and then start the multiple electric push rods 6 to drive the multiple placement seats 7, the limiting sleeves 8 and the test tube 9 to move upward until they pass through the opening on the partition 14, and then remove the limiting bolts on the fixing frame 10, and then remove the fixing frame 10 and take out the test tube 9. , further put multiple copies of stem cell exosomes to be preserved into multiple test tubes 9 respectively, and then place the test tubes 9 containing stem cell exosomes in the limiting sleeve 8 and the placement seat 7, and then seal the test tubes 9 through the test tube plugs 11 on the fixing frame 10 and fix the fixing frame 10 and the limiting sleeve 8 through the limiting bolts, and then plug the sealing plug 15 into the opening on the partition 14, and finally cover the box cover 16 on the box body 1 and fix it through the fixing bolts, so as to complete the preservation operation of the stem cell exosomes, and further, the four semiconductor manufacturing systems can be started to The cooling assembly 13 performs a cooling operation on the four heat conducting plates 12, and then utilizes the thermal conductivity of the heat conducting plates 12 to conduct cold air in the box 1, thereby refrigerating the stem cell exosomes stored in the box 1. The temperature in the box 1 can be further detected by starting the temperature sensor 5, and the detection result can be displayed on the display screen on the control panel 18. The output power of the semiconductor refrigeration assembly 13 can be adjusted through the control panel 18, and the cooling temperature of the semiconductor refrigeration assembly 13 in the box 1 can be adjusted. When personnel need to refrigerate it, When taking out a test tube 9 containing stem cell exosomes, the sealing plug 15 corresponding to the test tube 9 to be taken out can be further removed, and the electric push rod 6 can be further started to drive the designated placement seat 7, the limiting sleeve 8 and the test tube 9 to move upward and pass through the opening on the partition 14, and then the personnel can remove the test tube 9 to complete the taking operation of a single test tube 9 containing stem cell exosomes, thereby avoiding the situation that all the test tubes need to be exposed to room temperature when taking one of the test tubes, and further improving the storage effect of the stem cell exosomes.

[0025] Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art may still modify the technical solutions described in the aforementioned embodiments, or make equivalent substitutions for some of the technical features therein. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention shall be included in the protection scope of the present invention.

Claims

1. A stem cell exosome storage device, comprising a housing (1), Features: A battery (2) is fixedly installed at the bottom of the box (1), a support cover (3) is provided on the outer side of the battery (2), a fixing plate (4) is fixedly connected to the inner wall of the box (1), a plurality of temperature sensors (5) are fixedly embedded at equal distances on the top of the fixing plate (4), a plurality of electric push rods (6) are fixedly installed at equal distances on the inner bottom of the box (1), a placement seat (7) is fixedly connected to the push rod ends of the plurality of electric push rods (6), a placement slot is provided on the top of the placement seat (7), a limiting sleeve (8) is fixedly connected to the top of the placement seat (7) through a fixing rod, a test tube (9) is passed through the limiting sleeve (8), and the top of the limiting sleeve (8) is fixedly connected by a limiting bolt. A fixing frame (10) is fixedly connected, a test tube plug (11) is fixedly connected to the top of the inner side of the fixing frame (10), four heat conducting plates (12) are fixedly connected to the inner walls of the four sides of the box body (1), four semiconductor refrigeration components (13) are respectively installed through the four side walls of the box body (1), a partition (14) is fixedly connected to the inner wall of the box body (1), a plurality of openings of equal distance and size are opened on the partition (14) corresponding to the plurality of fixing frames (10), a sealing plug (15) is respectively plugged in the plurality of openings, the top of the box body (1) is open and is covered with a box cover (16), and a control panel (18) is fixedly installed on the front surface of the box body (1).

2. The stem cell exosome storage device according to claim 1, Features: The box body (1) has symmetrical heat dissipation mesh holes on its wall, the electric push rod (6) vertically penetrates the fixing plate (4), one end of the support cover (3) is fixedly connected to the bottom of the box body (1), the fixing plate (4) has a groove for fixing and embedding the temperature sensor (5), and the fixing plate (4) has a through hole for the electric push rod (6) to penetrate and connect.

3. The stem cell exosome storage device according to claim 1, Features: The limiting sleeve (8) is provided with a through hole for the test tube (9) to pass through and connect, the bottom end of the test tube (9) fits in the placement groove on the placement seat (7), the fixing frame (10) and the limiting sleeve (8) are both provided with threaded through holes for the limiting bolt to be threadedly connected, and one end of the test tube plug (11) is plugged inside the test tube (9).

4. The stem cell exosome storage device according to claim 1, Features: The box body (1) is provided with an opening for connecting a semiconductor refrigeration component (13) therethrough; the cooling side of the semiconductor refrigeration component (13) is fixedly connected to the outer side of the heat conducting plate (12); the box cover (16) is fixedly connected to the box body (1) via fixing bolts; threaded through holes for threaded connection with the fixing bolts are provided on the box cover (16) and the box body (1); the inner top of the box cover (16) fits with the top of the sealing plug (15).

5. The stem cell exosome storage device according to claim 1, Features: The top of the box cover (16) is symmetrically connected with a handle (17), and the control panel (18) is provided with a display screen and control buttons.

6. The stem cell exosome storage device according to claim 1, Features: The temperature sensor (5), the electric push rod (6) and the semiconductor refrigeration component (13) are electrically connected to the control panel (18) via wires, and the control panel (18) is electrically connected to the storage battery (2) via wires.

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