Centrifugal tube for cell centrifugal smear

Abstract

The utility model relates to the technical field of cell smears, in particular to a centrifugal tube for cell centrifugal smears, the centrifugal tube comprises a lower tube and an upper tube arranged on the lower tube, one end of the upper tube deviating from the lower tube is detachably connected with a sealing cover, a smear is arranged in the centrifugal tube through a limiting assembly, and the sealing cover is detachably connected with the sealing cover. The smear can adsorb cells, the smear is obliquely arranged in the centrifugal tube, the smear is arranged in the centrifugal tube, in the centrifugal working process, according to the Bernoulli principle, small cells are easily pulled to the middle of fluid due to the small mass, and large cells are correspondingly pushed to the two sides of the fluid due to the large mass and the large size, so that the small cells are easily pulled to the middle of the fluid; therefore, a large number of cells can be uniformly collected on the smear, the cells from large to small can be collected on the smear, and the relatively long longitudinal moving belt and the relatively long transverse moving belt can also be collected, so that a high-quality smear can be directly formed.

Description

Technical Field

[0001] The utility model relates to the technical field of cell smears, in particular to a centrifuge tube used for cell centrifugal smears. Background Art

[0002] Centrifuge tubes are tubular sample containers, which have been widely used in biological sciences, especially in the fields of biochemistry and molecular biology research. The centrifuge tubes currently used are generally traditional columnar centrifuge tubes, which are mainly used for the separation and preparation of various biological samples. The biological sample suspension is placed in the centrifuge tube. Under high-speed rotation, due to the huge centrifugal force, the suspended tiny particles settle at a certain speed, thereby being separated from the solution. The separated sediment is then used to make smears or cell wax blocks. However, the existing centrifuge tubes have the following problems:

[0003] 1-During the process of cell collection, it is difficult to take out the sediment at the bottom of the traditional columnar centrifuge tube, which makes it difficult to make cell wax blocks;

[0004] 2- During the cell collection process, large cells and small cells have different speeds, so during the collection process, large and small cells will produce a certain transition zone, in which there are a large number of nucleated cells with diagnostic significance. However, after centrifugation in traditional columnar centrifuge tubes, this transition zone is very short or even non-existent, and it is impossible to completely collect and make wax blocks, which brings difficulties to cell diagnosis;

[0005] 3- During the cytological diagnosis process, a cell smear that has not been fixed and dehydrated is needed for auxiliary examination. However, a good cell smear requires skilled technicians to operate, and also requires a large amount of sediment and cell transition zones in the sediment. However, the existing centrifuge tubes do not have the function of directly forming a high-quality smear. Utility Model Content

[0006] Based on this, the purpose of the utility model is to provide a centrifuge tube for cell centrifugation smear, which is convenient for taking out the sediment, can increase the extension of the transition zone, and can directly form a high-quality smear.

[0007] A centrifuge tube for cell centrifugal smear comprises a lower tube and an upper tube arranged on the lower tube, a sealing cover is detachably connected to one end of the upper tube away from the lower tube, a smear is arranged in the centrifuge tube through a limiting component, the smear can absorb cells, the smear is arranged in the centrifuge tube in an inclined manner, and the inner wall of the lower tube is adhered with lens paper for collecting centrifugal sediment.

[0008] Furthermore, the inner wall of the lower tube is provided with an internal thread, the upper tube is provided with an external thread, and the lower tube is connected to the external thread provided on the upper tube via the internal thread.

[0009] Furthermore, the lower tube is connected to the upper tube in a socket-type manner.

[0010] Furthermore, the smear includes a main body and a plurality of through holes opened on the main body.

[0011] Furthermore, the limiting assembly includes a first fixing block arranged in the upper tube and a slot opened on the first fixing block, and the upper part of the smear is located in the slot.

[0012] Furthermore, the limiting assembly includes a second fixing block arranged in the upper tube and a slide groove opened on the second fixing block, and the side of the smear is located in the slide groove.

[0013] Furthermore, the upper pipe is threadedly connected to the sealing cover.

[0014] Furthermore, the lens cleaning paper is provided with protrusions.

[0015] The utility model arranges a smear in a centrifuge tube. During the centrifugal operation, according to Bernoulli's principle, small cells are easily pulled to the middle of the fluid due to their small mass, while large cells are correspondingly pushed to both sides of the fluid due to their large mass and large volume. Therefore, a large number of cells can be collected evenly on the smear, and cells arranged from large to small can be collected on the smear, and relatively long longitudinal transition zones and transverse transition zones can also be collected, so that a high-quality smear can be directly formed. At the same time, lens cleaning paper is attached to the inner wall of the lower tube. When the centrifugal operation is completed, the precipitate can be taken out by simply taking the lens cleaning paper out of the lower tube, thereby achieving the effect of conveniently taking out the precipitate. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 It is a three-dimensional structural schematic diagram of the utility model.

[0017] Figure 2 It is a schematic diagram of the cross-sectional three-dimensional structure of the utility model.

[0018] Figure 3 It is a schematic diagram of the three-dimensional structure of the smear of the utility model.

[0019] Figure 4 It is a schematic diagram of the three-dimensional structure of the first fixing block of the utility model.

[0020] Figure 5 It is a schematic diagram of the lens cleaning paper and the raised three-dimensional structure of the utility model.

[0021] Figure 6 It is a schematic diagram of the three-dimensional structure of the second fixing block of the utility model.

[0022] Figure 7 It is a schematic diagram of the three-dimensional structure of the second fixing block and the sliding groove of the utility model.

[0023] Figure 8 It is a schematic diagram of the three-dimensional structure of the longitudinal transfer belt of the utility model.

[0024] Fig. 9 It is a schematic diagram of the three-dimensional structure of the transverse transfer belt of the utility model.

[0025] Fig.10 It is a schematic diagram of the three-dimensional structure of the smear sheet and the connecting sheet of the utility model.

[0026] Description of main component symbols:

[0027] 1-lower tube, 2-upper tube, 3-sealing cover, 41-lens cleaning paper, 42-protrusion, 5-smear, 51-main body, 52-through hole, 61-first fixed block, 62-slot, 63-second fixed block, 64-slide groove, 7-longitudinal transition zone, 8-lateral transition zone, 9-small cell, 10-large cell, 11-connecting piece, 111-connecting part, 112-protrusion.

[0028] The following specific implementation manner will further illustrate the present utility model in conjunction with the above-mentioned drawings. DETAILED DESCRIPTION

[0029] In order to facilitate the understanding of the present invention, the present invention will be described more fully below with reference to the relevant drawings. Several embodiments of the present invention are given in the drawings. However, the present invention can be implemented in many different forms and is not limited to the embodiments described herein. On the contrary, the purpose of providing these embodiments is to make the disclosure of the present invention more thorough and comprehensive.

[0030] It should be noted that when an element is referred to as being "fixed to" another element, it may be directly on the other element or there may be a central element. When an element is considered to be "connected to" another element, it may be directly connected to the other element or there may be a central element at the same time. The terms "vertical", "horizontal", "left", "right" and similar expressions used herein are for illustrative purposes only.

[0031] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as those commonly understood by those skilled in the art in the technical field of the present invention. The terms used herein in the specification of the present invention are only for the purpose of describing specific embodiments and are not intended to limit the present invention. The term "and / or" used herein includes any and all combinations of one or more related listed items.

[0032] A centrifuge tube for cytospin, such as Figure 1-Figure 5 and Figure 8-Figure 9 As shown, it includes a lower tube 1 and an upper tube 2 arranged on the lower tube 1, and a sealing cover 3 is detachably connected to the end of the upper tube 2 away from the lower tube 1. A smear 5 is arranged in the centrifuge tube through a limiting component, and the smear 5 can adsorb cells. The smear 5 is inclinedly arranged in the centrifuge tube, and the inner wall of the lower tube 1 is adhered with lens paper 41 for collecting centrifugal precipitation. The inner wall of the lower tube 1 is provided with an internal thread, and the upper tube 2 is provided with an external thread. The lower tube 1 is connected to the external thread opened on the upper tube 2 through the internal thread, and the upper tube 2 is threadedly connected to the sealing cover 3, and the smear 5 includes a main body 51 and a plurality of through holes 52 opened on the main body 51.

[0033] When using the centrifuge tube, first place the smear 5 into the upper tube 2 and limit the smear 5 with the limiting component, which can effectively prevent the smear 5 from shifting during the subsequent centrifugation process. Then the operator can rotate the lower tube 1 onto the upper tube 2, and the fixed connection between the lower tube 1 and the upper tube 2 is achieved through the internal thread of the lower tube 1 and the external thread of the upper tube 2. Then the operator can rotate and remove the sealing cover 3 from the upper tube 2. After removing the sealing cover 3 from the upper tube 2, the operator can pour the liquid to be centrifuged into the space formed by the lower tube 1 and the upper tube 2. After pouring the liquid to be centrifuged, the operator can reinstall the sealing cover 3 on the upper tube 2 and tighten the sealing cover 3 to prevent the liquid in the tube from leaking during the centrifugation. Then the operator places the centrifuge tube into the centrifuge for centrifugation. During the centrifugation, Figure 8-Figure 9As shown, when the liquid passes through the gap, the flow rate will be accelerated. Therefore, when the liquid passes through the through hole 52 and the gap between the smear 5 and the lower tube 1 or the upper tube 2, according to the Bernoulli principle, the small cells 9 are easily pulled to the middle of the fluid due to their small mass, while the large cells 10 are pushed to the two sides of the fluid due to their large mass and large volume. Therefore, a large number of cells can be collected evenly on the smear 5, and cells arranged from large to small can be collected on the smear 5. Also, relatively long longitudinal transition bands 7 and transverse transition bands 8 can be collected. Thus, a high-quality smear 5 can be directly formed, which is convenient for subsequent cell diagnosis. When the centrifugation work is completed, the separated precipitate will be located in the lower tube 1, and then the operator can take the centrifuge tube out of the centrifuge. After taking out the centrifuge tube, the operator removes the sealing cover 3 from the upper tube 2. After removing the sealing cover 3 Pour out the clear liquid above to keep the precipitate in the lower tube 1. Then the operator can separate the lower tube 1 from the upper tube 2, and then take out the smear 5 with collected cells. After the smear 5 is taken out, fit the main body 51 of the smear 5 with the connecting part 111 of the connecting piece 11, so that the protrusion 112 of the connecting piece 11 is located in the through hole 52 of the smear 5, thereby assembling the connecting piece 11 and the smear 5 together, and then drip neutral resin thereon, and then cover it with a cover glass, so that the cells on the smear 5 can be observed under a microscope. The operator can tear off the lens paper 41 from the inner wall of the lower tube 1 so that the lens paper 41 is no longer attached to the inner wall of the lower tube 1, and then take out the lens paper 41 wrapped with the precipitate from the lower tube 1, thereby facilitating the removal of the precipitate in the centrifuge tube. After the precipitate is taken out, it is fixed and dehydrated to finally form a pathological wax block.

[0034] Further description: In exfoliative cytology pathology diagnosis, the most important thing is to identify and collect target cells with diagnostic information, such as nucleated cells or tumor cells. These target cells usually have a relatively large volume and molecular weight. In contrast, non-target cells constitute the background of the cell sample, including anucleated red blood cells and components such as mucus proteins with smaller volume and molecular weight. The morphological characteristics, size, nuclear morphology, and nuclear-cytoplasmic ratio of target cells are crucial for the diagnosis and differential diagnosis of the disease, and provide important clues for diagnosis.

[0035] In summary, by arranging the smear 5 in the centrifuge tube, during the centrifugation process, according to the Bernoulli principle, small cells are easily pulled to the middle of the fluid due to their small mass, while large cells are pushed to both sides of the fluid due to their large mass and large volume. Therefore, a large number of cells can be collected evenly on the smear 5, and cells arranged from large to small can be collected on the smear 5, and relatively long longitudinal transition bands 7 and transverse transition bands 8 can also be collected, thereby directly forming a high-quality cell smear 5. At the same time, by attaching the lens paper 41 to the inner wall of the lower tube 1, when the centrifugation work is completed, the precipitate can be removed by simply taking out the lens paper 41 from the lower tube 1, thereby achieving the effect of conveniently removing the precipitate.

[0036] like Figure 4 As shown, the limiting assembly includes a first fixing block 61 disposed in the upper tube 2 and a slot 62 opened on the first fixing block 61 , and the upper portion of the smear 5 is located in the slot 62 .

[0037] When the operator needs to place the smear 5 into the centrifuge tube, the operator can place the upper part of the smear 5 into the slot 62. Through the setting of the slot 62, the smear 5 will not move when the centrifuge tube is centrifuged, so that the smear 5 is stable when collecting cells.

[0038] like Figure 5 As shown, the lens cleaning paper 41 is provided with a protrusion 42 .

[0039] Among them, by providing a protrusion on the lens cleaning paper 41, when the lens cleaning paper 41 needs to be taken out from the lower tube 1, the lens cleaning paper 41 can be easily peeled off from the inner wall of the lower tube 1 by simply pulling the protrusion. At the same time, when the lower tube 1 and the upper tube 2 are combined together, the upper tube 2 presses the protrusion 42 to prevent the liquid from pushing the protrusion 42 during centrifugal operation, resulting in the lens cleaning paper 41 and the inner wall of the lower tube 1 not fitting tightly.

[0040] The second embodiment of the utility model is different from the first embodiment in that the lower tube 1 is connected to the upper tube 2 in a socket-type manner.

[0041] The operator can separate and install the upper tube 2 and the lower tube 1 by plugging and unplugging.

[0042] like Figure 6-Figure 7 As shown, the third embodiment of the utility model is different from the first embodiment in that the limiting assembly includes a second fixed block 63 arranged in the upper tube 2 and a slide groove 64 opened on the second fixed block 63, and the side of the smear 5 is located in the slide groove 64.

[0043] When the operator needs to place the smear 5 into the centrifuge tube, the operator can slide the side of the smear 5 into the slide groove 64. Through the setting of the slide groove 64, the smear 5 will not move when the centrifuge tube is centrifuged, so that the smear 5 is stable when collecting cells.

[0044] In the description of this specification, the description with reference to the terms "one embodiment", "some embodiments", "example", "specific example", or "some examples" means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the utility model. In this specification, the schematic representation of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any one or more embodiments or examples in a suitable manner.

[0045] The above-mentioned embodiments only express several implementation methods of the utility model, and the descriptions thereof are relatively specific and detailed, but they cannot be understood as limiting the scope of the patent of the utility model. It should be pointed out that, for ordinary technicians in this field, several variations and improvements can be made without departing from the concept of the utility model, and these all belong to the protection scope of the utility model. Therefore, the protection scope of the utility model patent shall be based on the attached claims.

Claims

1. A centrifuge tube for cytocentrifugation, It is characterized in that It comprises a lower tube and an upper tube arranged on the lower tube, wherein a sealing cover is detachably connected to one end of the upper tube away from the lower tube, a smear is arranged in the centrifuge tube through a limiting component, the smear can adsorb cells, the smear is arranged in an inclined manner in the centrifuge tube, and the inner wall of the lower tube is adhered with lens paper for collecting centrifugal sediment.

2. The centrifuge tube according to claim 1, It is characterized in that The inner wall of the lower tube is provided with an internal thread, the upper tube is provided with an external thread, and the lower tube is connected with the external thread provided on the upper tube through the internal thread.

3. The centrifuge tube according to claim 1, It is characterized in that The lower tube is connected to the upper tube in a socket-and-spigot manner.

4. The centrifuge tube according to claim 1, It is characterized in that The smear comprises a main body and a plurality of through holes opened in the main body.

5. The centrifuge tube according to claim 1, It is characterized in that The limiting assembly includes a first fixing block arranged in the upper tube and a slot opened on the first fixing block, and the upper part of the smear is located in the slot.

6. The centrifuge tube according to claim 1, It is characterized in that The limiting assembly includes a second fixing block arranged in the upper tube and a slide groove opened on the second fixing block, and the side of the smear is located in the slide groove.

7. The centrifuge tube according to claim 1, It is characterized in that The upper pipe is threadedly connected with the sealing cover.

8. The centrifuge tube according to claim 1, It is characterized in that The lens cleaning paper is provided with protrusions.

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