Gene sequencing equipment

Abstract

The utility model belongs to the technical field of gene sequencing, and discloses gene sequencing equipment. The gene sequencing equipment comprises an optical detection device, a fluid storage device and a fluid suction device, the optical detection device is used for performing optical detection on a fluorescence signal of a to-be-detected gene segment fixed in a flow cell, and the fluid storage device comprises a sample library storage mechanism, a reagent storage mechanism and a cleaning agent storage mechanism; the fluid suction device comprises a driving mechanism and a suction mechanism, the suction mechanism comprises a mounting part and a sample suction needle, a reagent suction needle and a cleaning agent suction needle which are arranged on the mounting part, and the sample suction needle, the reagent suction needle and the cleaning agent suction needle are all communicated with the flow cell; the sample suction needle, the reagent suction needle and the cleaning agent suction needle are arranged corresponding to the sample library storage mechanism, the reagent storage mechanism and the cleaning agent storage mechanism, and the driving mechanism is used for driving the suction mechanism to move towards or away from the fluid storage device, so that the structure is simple, the operation is convenient, the detection efficiency is high, and the cost is low.

Description

Technical Field

[0001] The utility model relates to the technical field of gene sequencing, in particular to a gene sequencing device. Background Art

[0002] Gene sequencing is a new gene testing technology that can analyze and determine the entire gene sequence from blood or saliva to predict the possibility of suffering from various diseases. A gene sequencing device is a device that can perform gene sequencing. The gene sequencing device includes an optical detection device, a fluid storage device, and a fluid suction device. The fluid storage device is used to store fluids such as sample libraries, reagents, and cleaning agents required for the test, and the gene sequencing device uses a fluid suction device to absorb these fluids into a flow pool. The fluid undergoes a biochemical reaction in the flow pool. After the biochemical reaction is completed, the optical detection device can optically detect the fluorescent signal of the gene fragment to be tested fixed in the flow pool, thereby obtaining the gene test results.

[0003] The fluid suction device of the existing gene sequencing equipment can generally only control the suction of one fluid at a time. This configuration requires a corresponding fluid suction device to be set up for each fluid, or each fluid is sucked into the flow pool by a fluid suction device in turn. This will cause the fluid suction device to have a complex structure and inconvenient operation, and the detection efficiency is low, which will also increase the cost of the gene sequencing equipment.

[0004] Therefore, a new gene sequencing device is urgently needed to solve the problems of complex structure, inconvenient operation and high cost. Utility Model Content

[0005] The utility model aims to provide a gene sequencing device with a simple structure, convenient operation, high detection efficiency and low cost.

[0006] To achieve this purpose, the utility model adopts the following technical solutions:

[0007] Gene sequencing equipment, including:

[0008] An optical detection device, used for optically detecting the fluorescent signal of the gene fragment to be detected fixed in the flow cell;

[0009] A fluid storage device, including a sample library storage mechanism, a reagent storage mechanism, and a cleaning agent storage mechanism;

[0010] A fluid suction device comprises a driving mechanism and a suction mechanism, wherein the suction mechanism comprises a mounting part and a sample suction needle, a reagent suction needle and a cleaning agent suction needle arranged on the mounting part, wherein the sample suction needle, the reagent suction needle and the cleaning agent suction needle are all connected to the flow pool, and the sample suction needle, the reagent suction needle and the cleaning agent suction needle are arranged correspondingly to the sample library storage mechanism, the reagent storage mechanism and the cleaning agent storage mechanism, and the driving mechanism is used to drive the suction mechanism to move toward or away from the fluid storage device so as to absorb the fluid stored in the fluid storage device into the flow pool.

[0011] As a preferred embodiment, the fluid suction device further comprises:

[0012] The first guide mechanism comprises a first guide rail and a first slider, the first guide rail extends along the movement direction of the suction mechanism, the first slider is slidably connected to the first guide rail, and the first slider is connected to the suction mechanism.

[0013] As a preferred embodiment, the fluid suction device further comprises:

[0014] The first position detection mechanism is used to detect the position of the suction mechanism in the moving direction.

[0015] As a preferred solution, the fluid storage device further comprises:

[0016] A temperature control mechanism, used to control the temperature of the reagent storage mechanism;

[0017] The condensate pump is used to discharge the condensed water generated in the reagent storage mechanism due to temperature control.

[0018] As a preferred solution, the optical detection device comprises:

[0019] A carrying mechanism, used for carrying and fixing the flow cell;

[0020] A light source mechanism, used for emitting excitation light to the flow cell;

[0021] The collecting mechanism is used to collect the fluorescent signal of the gene fragment to be tested fixed in the flow cell.

[0022] As a preferred solution, the optical detection device further comprises:

[0023] The air extraction mechanism is used to provide the required negative pressure for the supporting mechanism.

[0024] As a preferred solution, the bearing mechanism includes:

[0025] A carrying platform, used for carrying and fixing the flow cell;

[0026] The moving component is used to drive the supporting platform to move or rotate.

[0027] As a preferred solution, the gene sequencing device further includes:

[0028] A cooling device is used to dissipate heat for the temperature control component in the supporting mechanism.

[0029] As a preferred solution, the gene sequencing device further includes:

[0030] A box body, wherein the optical detection device, the fluid storage device and the fluid suction device are all located in the box body, the box body is provided with an opening, and the box body is also provided with a display screen, and the display screen can block the opening or open the opening;

[0031] The driving device is used for driving the display screen to move toward or away from the opening.

[0032] As a preferred solution, the box body is provided with a safety protection member, and the safety protection member is used to detect whether the opening is blocked.

[0033] Beneficial effects:

[0034] The utility model provides a gene sequencing device, wherein a fluid suction device of the device comprises a driving mechanism and a suction mechanism, wherein the suction mechanism comprises a mounting part and a sample suction needle, a reagent suction needle and a cleaning agent suction needle arranged on the mounting part, wherein the sample suction needle, the reagent suction needle and the cleaning agent suction needle are all connected to a flow pool, and the sample suction needle, the reagent suction needle and the cleaning agent suction needle are arranged correspondingly to a sample library storage mechanism, a reagent storage mechanism and a cleaning agent storage mechanism, and the driving mechanism is used to drive the suction mechanism to move toward or away from a fluid storage device to suck the fluid stored in the fluid storage device into the flow pool, so that when performing a test, one driving mechanism can simultaneously drive the suction mechanism to suck all kinds of fluids, without setting a corresponding driving mechanism and suction mechanism for each fluid, thereby greatly simplifying the structure of the gene sequencing device and reducing the cost. In addition, one driving mechanism is simpler to set and operate than multiple driving mechanisms, and has a higher detection efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0035] Figure 1 It is a structural schematic diagram of a fluid storage device and a fluid suction device provided by an embodiment of the utility model;

[0036] Figure 2 It is a structural schematic diagram of an optical detection device provided by an embodiment of the utility model;

[0037] Figure 3 It is a schematic diagram of the structure of the cooling device provided by the embodiment of the utility model;

[0038] Figure 4 It is a structural schematic diagram of a gene sequencing device provided by an embodiment of the utility model;

[0039] Figure 5 It is a structural schematic diagram of a display screen and a driving device provided by an embodiment of the utility model;

[0040] Figure 6 It is a structural schematic diagram of an interface component provided by an embodiment of the utility model;

[0041] Figure 7 It is a structural schematic diagram of an electrical control device provided in an embodiment of the utility model.

[0042] In the figure:

[0043] 10. Optical detection device; 11. Carrying mechanism; 111. Carrying platform; 112. Moving assembly; 12. Light source mechanism; 13. Collection mechanism; 14. Air extraction mechanism;

[0044] 20. Fluid storage device; 21. Sample library storage mechanism; 22. Reagent storage mechanism; 23. Cleaning agent storage mechanism; 24. Temperature control mechanism; 25. Condensate pump;

[0045] 30. Fluid suction device; 31. Driving mechanism; 32. Suction mechanism; 321. Mounting member; 322. Sample suction needle; 323. Reagent suction needle; 324. Cleaning agent suction needle; 33. First guide mechanism; 331. First guide rail; 332. First slider; 34. First position detection mechanism; 35. Suction liquid pump; 36. Selection valve;

[0046] 40. Cooling device; 41. Water tank; 42. Fan; 43. Cooling radiator; 44. Extraction pump;

[0047] 50. Electrical control device; 51. Computer mechanism; 52. DC power supply mechanism; 53. Power distribution mechanism; 54. Relay;

[0048] 60. driving device; 61. driving member; 62. gear mechanism; 63. rack; 64. second position detection mechanism; 65. second guide mechanism;

[0049] 70. Box body; 71. Opening; 72. Display screen; 73. Shielding member; 74. Safety protection member; 75. Switch; 76. Interface assembly; 761. Waste liquid interface; 762. Waste liquid signal interface; 77. Heat sink. DETAILED DESCRIPTION

[0050] The present invention is further described in detail below in conjunction with the accompanying drawings and embodiments. It is to be understood that the specific embodiments described herein are only used to explain the present invention, rather than to limit the present invention. It should also be noted that, for ease of description, only the parts related to the present invention, rather than all structures, are shown in the accompanying drawings.

[0051] In the description of the present invention, unless otherwise clearly specified and limited, the terms "connected", "connected", and "fixed" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0052] In the present utility model, unless otherwise clearly specified and limited, a first feature being "above" or "below" a second feature may include that the first and second features are in direct contact, or may include that the first and second features are not in direct contact but are in contact through another feature between them. Moreover, a first feature being "above", "above" and "above" a second feature includes that the first feature is directly above and obliquely above the second feature, or simply indicates that the first feature is higher in level than the second feature. A first feature being "below", "below" and "below" a second feature includes that the first feature is directly below and obliquely below the second feature, or simply indicates that the first feature is lower in level than the second feature.

[0053] In the description of this embodiment, the terms "upper", "lower", "left", "right" and other directions or positional relationships are based on the directions or positional relationships shown in the drawings, and are only for the convenience of description and simplified operation, rather than indicating or implying that the device or element referred to must have a specific direction, be constructed and operated in a specific direction, and therefore cannot be understood as a limitation of the present invention. In addition, the terms "first" and "second" are only used to distinguish in the description and have no special meaning.

[0054] Gene sequencing is a new type of gene testing technology that can analyze and determine the entire gene sequence from blood or saliva to predict the possibility of suffering from various diseases. Gene sequencing equipment is a device that can perform gene sequencing. Figure 1 and Figure 2As shown, this embodiment provides a gene sequencing device, which includes an optical detection device 10, a fluid storage device 20 and a fluid suction device 30. The optical detection device 10 is used to optically detect the fluorescent signal of the gene fragment to be detected fixed in the flow pool. The fluid storage device 20 includes a sample library storage mechanism 21, a reagent storage mechanism 22 and a cleaning agent storage mechanism 23. The fluid suction device 30 includes a driving mechanism 31 and a suction mechanism 32. The suction mechanism 32 includes a mounting member 321 and a sample suction device disposed on the mounting member 321. The sampling needle 322, the reagent suction needle 323 and the cleaning agent suction needle 324 are all connected to the flow pool. The sample suction needle 322, the reagent suction needle 323 and the cleaning agent suction needle 324 are arranged corresponding to the sample library storage mechanism 21, the reagent storage mechanism 22 and the cleaning agent storage mechanism 23. The driving mechanism 31 is used to drive the suction mechanism 32 to move toward or away from the fluid storage device 20, so as to absorb the fluid stored in the fluid storage device 20 into the flow pool.

[0055] When the gene sequencing device is testing, one driving mechanism 31 can simultaneously drive the suction mechanism 32 to suck all kinds of fluids, without having to set up a corresponding driving mechanism 31 and suction mechanism 32 for each fluid, thereby greatly simplifying the structure of the gene sequencing device and reducing the cost of the gene sequencing device. In addition, setting up one driving mechanism 31 is simpler to operate than setting up multiple driving mechanisms 31, and the detection efficiency is also higher.

[0056] In addition, if Figure 1 As shown, in the present embodiment, the driving mechanism 31 is a structure driven by a rotating motor and a screw-nut assembly. The driving of this structure is stable and has high motion accuracy. In other embodiments, the driving mechanism 31 can also be a structure with simple structure and low cost, such as a cylinder. All other structures that can realize the driving function are within the protection scope of this embodiment.

[0057] like Figure 1 As shown, the fluid storage device 20 also includes a temperature control mechanism 24, which is used to control the temperature of the reagent storage mechanism 22, so that the reagent storage mechanism 22 is maintained at a relatively low temperature, thereby ensuring the stability and use effect of the reagent stored inside. In this embodiment, the temperature control mechanism 24 can be any temperature control structure in the prior art, and this embodiment does not limit this.

[0058] In addition, if Figure 1As shown, since condensed water will be generated in the reagent storage mechanism 22 during the process of the temperature control mechanism 24 controlling the temperature of the reagent storage mechanism 22, the fluid storage device 20 is also provided with a condensate pump 25, which is used to discharge the condensed water generated in the reagent storage mechanism 22 due to temperature control, thereby avoiding the accumulation of condensed water in the reagent storage mechanism 22 and causing damage to the equipment. In this embodiment, the condensate pump 25 is a water pump, which has a simple structure, low cost and good extraction effect. In other embodiments, a guide groove or other structure may be provided to discharge the condensed water in the reagent storage mechanism 22, which is not limited in this embodiment.

[0059] like Figure 1 As shown, the fluid suction device 30 also includes a suction pump 35, which is connected to the sample suction needle 322, the reagent suction needle 323 and the cleaning agent suction needle 324, so that the suction pump 35 can suck the fluid in the fluid storage device 20 and transport the fluid to the flow pool. This suction method is easy to operate, precise in control and quick in response. In this embodiment, the suction pump 35 is a syringe pump.

[0060] In addition, if Figure 1 As shown, the fluid suction device 30 also includes a selection valve 36, which is arranged on the fluid passage and used to control the on-off of the fluid passage. The gene sequencing device also includes a control system, and the selection valve 36 is communicatively connected with the control system. The control system can selectively control the on-off of the selection valve 36 according to the progress of the detection, so as to accurately control the required fluid to be sucked into the flow pool, ensuring the smooth progress of the detection process. In this embodiment, the selection valve 36 is a rotary switching valve.

[0061] like Figure 1 As shown, the fluid suction device 30 further includes a first guide mechanism 33, which includes a first guide rail 331 and a first slider 332. The first guide rail 331 extends along the movement direction of the suction mechanism 32, and the first slider 332 is slidably connected to the first guide rail 331, and the first slider 332 is connected to the mounting member 321. The first guide mechanism 33 can guide the movement of the suction mechanism 32 to prevent the suction mechanism 32 from being deflected during the movement, thereby ensuring that the sample suction needle 322, the reagent suction needle 323, and the cleaning agent suction needle 324 are accurately inserted into the corresponding fluid storage device 20.

[0062] like Figure 1As shown, the fluid suction device 30 also includes a first position detection mechanism 34, which is used to detect the position of the mounting member 321 in the direction of movement. The first position detection mechanism 34 and the drive mechanism 31 are also connected to the control system in communication. When the first position detection mechanism 34 detects that the mounting member 321 moves to the specified position, it transmits a signal to the control system, and the control system controls the drive mechanism 31 to stop driving, thereby ensuring that the mounting member 321 stops at the specified position, thereby ensuring that the suction mechanism 32 can completely move to the bottom of the fluid storage device 20, so as to completely absorb the fluid, and will not damage the fluid storage device 20 or other structures due to the long movement distance. In this embodiment, the suction mechanism 32 specifically moves in the up and down directions, and two first position detection mechanisms 34 are arranged in the up and down directions, so that the upper limit position and the lower limit position of the suction mechanism 32 can be accurately controlled. In this embodiment, the first position detection mechanism 34 is a photoelectric sensor, and a shielding portion is provided on the mounting member 321. When the mounting member 321 moves to the position of the photoelectric sensor, the light emitted by the photoelectric sensor will be shielded by the shielding portion, so that the photoelectric sensor senses the position of the mounting member 321. The photoelectric sensor is sensitive and the control is rapid. In other embodiments, the first position detection mechanism 34 can also be a potentiometric position sensor or a capacitive position sensor, etc. All other structures that can realize the position detection function are within the protection scope of this embodiment.

[0063] like Figure 2 As shown, the optical detection device 10 includes a carrying mechanism 11, a light source mechanism 12 and a collection mechanism 13. The carrying mechanism 11 is used to carry and fix the flow cell, the light source mechanism 12 is used to emit excitation light to the flow cell, and the collection mechanism 13 is used to collect the fluorescence signal of the gene fragment to be tested fixed in the flow cell. After the biochemical reaction in the flow cell is completed, the light source mechanism 12 emits excitation light to the flow cell to induce the fluorescence signal of the gene fragment to be tested fixed in the flow cell, and then the collection mechanism 13 can collect the fluorescence signal to obtain the signal required for the detection result.

[0064] like Figure 2 As shown, the bearing mechanism 11 includes a bearing platform 111 and a moving component 112, the bearing platform 111 is used to bear the flow pool, and the moving component 112 is used to drive the bearing platform 111 to move or rotate. During the shooting process of the acquisition mechanism 13, the driving mechanism 31 simultaneously drives the bearing platform 111 to move or rotate, thereby ensuring that the acquisition mechanism 13 can collect the fluorescent signal in the flow pool. In this embodiment, the driving mechanism 31 can be a rotary motor drive in conjunction with a screw nut drive to drive the bearing platform 111 to move, or a cylinder or other structure to drive the bearing platform 111 to move, or it can be a structure such as the XXY alignment platform in the prior art that can drive the bearing platform 111 to move and drive the bearing platform 111 to rotate, and this embodiment does not limit this.

[0065] like Figure 2 and Figure 3 As shown, the optical detection device 10 also includes an air extraction mechanism 14, which is used to provide the required negative pressure for the carrying mechanism 11. The air extraction mechanism 14 can provide negative pressure for the carrying platform 111, and then firmly adsorb the flow cell on the carrying platform 111 through the negative pressure, thereby avoiding the relative displacement between the flow cell and the carrying platform 111 when the moving component 112 drives the carrying platform 111 to move or rotate, resulting in inaccurate collection by the collection mechanism 13, thereby ensuring the accuracy of the detection. In this embodiment, the air extraction mechanism 14 is a vacuum air pump or vacuum generator in the prior art, which will not be described in detail in this embodiment.

[0066] like Figure 2 and Figure 3 As shown, the gene sequencing device also includes a cooling device 40, which is used to dissipate heat from the temperature control component in the supporting mechanism 11, thereby ensuring that the flow pool on the supporting platform 111 is maintained at a suitable temperature, providing the required temperature conditions for normal biochemical reactions in the flow pool.

[0067] Specifically, if Figure 2 and Figure 3 As shown, the cooling device 40 includes a Peltier, a cold radiator 43, a water tank 41, an extraction pump 44 and a fan 42. The cold end of the Peltier is in close contact with the support platform 111, so as to cool the support platform 111. The extraction pump 44 draws cooling water from the water tank 41 to cool the hot end of the Peltier. When the cooling water circulates to the cold radiator 43, the fan 42 cools the cold radiator 43, thereby ensuring that the cooling water can maintain a low temperature state, thereby ensuring the cooling effect of the Peltier on the support platform 111.

[0068] like Figure 4 and Figure 5 As shown, the gene sequencing device also includes a box 70 and a driving device 60. The optical detection device 10, the fluid storage device 20 and the fluid suction device 30 are all located in the box 70. The box 70 is provided with an opening 71. The box 70 is also provided with a display screen 72. The display screen 72 can block the opening 71 or open the opening 71. The driving device 60 is used to drive the display screen 72 to move toward or away from the opening 71. Specifically, as Figure 4As shown, inside the box 70, the fluid suction device 30 is arranged below the optical detection device 10, and the display screen 72 and the opening 71 are set at a position on the box 70 directly opposite the optical detection device 10. Before the detection starts, the operator is required to place the flow pool from the opening 71 to the support table 111, and then the driving mechanism 31 drives the display screen 72 to block the opening 71, so as to prevent the excitation light emitted by the light source mechanism 12 from affecting the operator or the surrounding environment, and at the same time, it also ensures that the detection process is carried out in a relatively closed environment inside the box 70, thereby ensuring the accuracy of the detection results.

[0069] Specifically, if Figure 5 As shown, the driving device 60 includes a driving member 61, a gear mechanism 62 and a rack 63. The output end of the driving device 60 is connected to the gear mechanism 62, and the gear mechanism 62 is meshed with the rack 63. The display screen 72 is arranged on the rack 63, so that the output end of the driving member 61 rotates to drive the gear mechanism 62 to rotate, and then drives the rack 63 to move in the up and down directions, thereby driving the display screen 72 to open or block the opening 71. This driving method is stable and low in cost. The driving member 61 is specifically a rotating motor. In other embodiments, the gear mechanism 62 and the rack 63 can also be a structure with stable transmission and precise control such as a screw nut assembly, or a structure with simple structure and low cost such as a cylinder. This embodiment does not limit this.

[0070] In addition, if Figure 5 As shown, the driving device 60 also includes a second guiding mechanism 65, and the second guiding mechanism 65 includes a second guide rail and a second slider. The second guide rail extends in the up-down direction, and the second slider is connected to the second guide rail slider, and the second slider is connected to the display screen 72. The second guiding mechanism 65 can guide the up-down movement of the display screen 72, thereby ensuring that the display screen 72 does not deflect during the up-down movement, thereby ensuring that the display screen 72 can smoothly close and open the opening 71, and ensuring that the appearance of the gene sequencing device is neat and beautiful.

[0071] In addition, if Figure 5As shown, the driving device 60 also includes a second position detection mechanism 64, which is used to detect the position of the display screen 72 in the up and down direction. When the second position detection mechanism 64 and the driving device 60 are also connected to the control system, when the second position detection mechanism 64 detects that the display screen 72 moves to the specified position, it transmits a signal to the control system, and the control system controls the driving device 60 to stop driving, thereby ensuring that the display screen 72 stops at the specified position, thereby ensuring that the display screen 72 can completely block the opening 71, and will not affect other structures or the aesthetics of the entire device due to the long movement distance. In this embodiment, the second position detection mechanism 64 is arranged on the box body 70, and two are arranged along the up and down direction, so that the upper limit position and the lower limit position of the display screen 72 can be accurately controlled. In this embodiment, the second position detection mechanism 64 is a photoelectric sensor, and a shielding member 73 is arranged on the display screen 72. When the display screen 72 moves to the position of the photoelectric sensor, the light emitted by the photoelectric sensor will be blocked by the shielding member 73, so that the photoelectric sensor senses the position of the display screen 72, and the photoelectric sensor is sensitive and controlled quickly. In other embodiments, the second position detection mechanism 64 may also be a potential position sensor or a capacitive position sensor, etc. All structures that can realize the position detection function are within the protection scope of the embodiments of the present disclosure.

[0072] In addition, if Figure 4 and Figure 5 As shown, the box 70 is also provided with a switch 75, and the switch 75 facilitates the operator to control the display screen 72 to move upward or downward.

[0073] like Figure 5 As shown, the box 70 is provided with a safety protection member 74, which is used to detect whether the opening 71 is blocked. The safety protection member 74 is connected to the control system. When it is detected that there is an obstruction at the opening 71, the safety protection member 74 transmits a signal to the control system, and the control system controls the drive device 60 to stop driving the display screen 72 to move until the safety protection member 74 detects that there is no obstruction at the opening 71 and then continues to move, thereby preventing the display screen 72 from hitting the obstruction and causing damage to the display screen 72, and also preventing the display screen 72 from starting to move when the operator or other objects are still in the opening 71, thereby causing the operator to be pinched or other objects to be damaged. In the embodiment of the present disclosure, the safety protection member 74 is a grating, which has a fast response speed and high sensing accuracy. In other embodiments, the safety protection member 74 can also be a precise sensing structure such as an infrared detector. All other structures that can achieve safety protection functions are within the protection scope of this embodiment.

[0074] like Figure 4 and Figure 6As shown, the housing 70 is also provided with an interface component 76, and the interface component 76 is provided with a plurality of interfaces, which can be used for external power supply of the gene detection device, connecting network cables, external image signals and other required equipment. Among them, the interface component 76 is provided with a waste liquid interface 761, and the waste liquid interface 761 is connected to the flow cell. When the detection work in the flow cell is completed, the waste liquid will be collected by the waste liquid interface 761 to the waste liquid recovery device outside, which is convenient for subsequent centralized and unified treatment to avoid environmental pollution. At the same time, the interface component 76 is also provided with a waste liquid signal interface 762, and a position detection component is provided in the waste liquid recovery device. The waste liquid signal interface 762 and the control system are both connected to the position detection component for communication. The position detection component can detect the liquid height in the waste liquid recovery device. When the height of the waste liquid is detected to reach the specified position, a signal is transmitted to the control system to notify the operator, and the operator can handle the waste liquid according to the actual situation, thereby avoiding the waste liquid height exceeding the waste liquid recovery device and overflowing into the external environment. In this embodiment, the waste liquid detection component can be a photoelectric sensor, etc., as long as it can successfully detect the waste liquid height and transmit a signal to the control system, and this embodiment does not limit this.

[0075] In addition, if Figure 4 As shown, the box 70 is also provided with a heat sink 77, which can transfer the heat inside the box 70, thereby ensuring that the temperature inside the box 70 is appropriate, and then ensuring that the structure inside the box 70 works normally. In this embodiment, the heat sink 77 is a fan, which has a simple structure and low cost. In other embodiments, the heat sink 77 can also be a structure such as a heat sink fin, which is not limited in this embodiment.

[0076] In addition, the box 70 is also externally connected to operating devices such as a keyboard and a mouse, which facilitates the operator to perform input operations on the gene sequencing device.

[0077] like Figure 7 As shown, the gene sequencing device also includes an electrical control device 50, which is used to provide power support and control system for the entire device. The electrical control device 50 includes a computer mechanism 51, a DC power supply mechanism 52, a power distribution mechanism 53 and a relay 54. The computer mechanism 51 provides physical support for the control system, the DC power supply mechanism 52 and the power distribution mechanism 53 provide power support for various components in the device, and the relay 54 can protect the entire circuit.

[0078] Combine the following Figure 1 , Figure 2 , Figure 4 and Figure 5 The workflow of the gene sequencing equipment is described below:

[0079] First, if Figure 4 and Figure 5As shown, the driving device 60 drives the display screen 72 to move upward to open the opening 71;

[0080] Secondly, if Figure 2 , Figure 4 and Figure 5 As shown, the operator places the flow cell on the carrier 111 through the opening 71;

[0081] Then, if Figure 1 As shown, the driving mechanism 31 drives the suction mechanism 32 to move toward the direction close to the fluid storage device 20;

[0082] Then, if Figure 1 As shown, the sample aspirating needle 322 aspirates the sample from the sample library storage mechanism 21 to the flow cell, and the reagent aspirating needle 323 aspirates the reagent from the reagent aspirating mechanism 32 to the flow cell;

[0083] Then, if Figure 2 As shown, biochemical reactions occur in the flow cell;

[0084] Then, if Figure 2 As shown, the light source mechanism 12 emits excitation light to the flow cell;

[0085] Finally, if Figure 2 As shown, the collecting mechanism 13 collects the fluorescent signal of the gene fragment to be tested fixed in the flow pool, and at the same time the moving component 112 drives the supporting platform 111 to move or rotate.

[0086] Obviously, the above embodiments of the present invention are merely examples for the purpose of clearly illustrating the present invention, and are not intended to limit the implementation methods of the present invention. For those skilled in the art, various obvious changes, readjustments and substitutions can be made without departing from the scope of protection of the present invention. It is not necessary and impossible to list all implementation methods here. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention shall be included in the scope of protection of the claims of the present invention.

Claims

1. A gene sequencing device, characterized in that: include: An optical detection device (10) is used to optically detect the fluorescent signal of the gene fragment to be detected fixed in the flow cell; A fluid storage device (20), comprising a sample library storage mechanism (21), a reagent storage mechanism (22) and a cleaning agent storage mechanism (23); A fluid aspirating device (30) comprises a driving mechanism (31) and an aspirating mechanism (32). The aspirating mechanism (32) comprises a mounting member (321) and a sample aspirating needle (322), a reagent aspirating needle (323) and a cleaning agent aspirating needle (324) arranged on the mounting member (321). The sample aspirating needle (322), the reagent aspirating needle (323) and the cleaning agent aspirating needle (324) are all connected to the flow pool. The sample aspirating needle (322), the reagent aspirating needle (323) and the cleaning agent aspirating needle (324) are arranged correspondingly to the sample library storage mechanism (21), the reagent storage mechanism (22) and the cleaning agent storage mechanism (23). The driving mechanism (31) is used to drive the aspirating mechanism (32) to move in a direction approaching or away from the fluid storage device (20) so as to aspirate the fluid stored in the fluid storage device (20) into the flow pool.

2. The gene sequencing device according to claim 1, characterized in that: The fluid suction device (30) further comprises: A first guide mechanism (33), the first guide mechanism (33) comprising a first guide rail (331) and a first slider (332), the first guide rail (331) extending along the movement direction of the suction mechanism (32), the first slider (332) being slidably connected to the first guide rail (331), and the first slider (332) being connected to the suction mechanism (32).

3. The gene sequencing device according to claim 1, characterized in that: The fluid suction device (30) further comprises: The first position detection mechanism (34) is used to detect the position of the suction mechanism (32) in the moving direction.

4. The gene sequencing device according to claim 1, characterized in that: The fluid storage device (20) further comprises: A temperature control mechanism (24) for controlling the temperature of the reagent storage mechanism (22); The condensate pump (25) is used to discharge condensed water generated in the reagent storage mechanism (22) due to temperature control.

5. The gene sequencing device according to any one of claims 1 to 4, characterized in that: The optical detection device (10) comprises: A carrying mechanism (11), used for carrying and fixing the flow cell; A light source mechanism (12) for emitting excitation light to the flow cell; The collecting mechanism (13) is used to collect the fluorescent signal of the gene fragment to be tested fixed in the flow cell.

6. The gene sequencing device according to claim 5, characterized in that: The optical detection device (10) further comprises: The air extraction mechanism (14) is used to provide the required negative pressure for the supporting mechanism (11).

7. The gene sequencing device according to claim 5, characterized in that: The carrying mechanism (11) comprises: A carrying platform (111), used for carrying and fixing the flow cell; The moving component (112) is used to drive the supporting platform (111) to move or rotate.

8. The gene sequencing device according to claim 5, characterized in that: The gene sequencing device also includes: A cooling device (40) is used to dissipate heat for the temperature control component in the supporting mechanism (11).

9. The gene sequencing device according to any one of claims 1 to 4, characterized in that: The gene sequencing device also includes: A box (70), wherein the optical detection device (10), the fluid storage device (20) and the fluid suction device (30) are all located in the box (70), the box (70) is provided with an opening (71), and the box (70) is also provided with a display screen (72), and the display screen (72) can block the opening (71) or open the opening (71); The driving device (60) is used to drive the display screen (72) to move toward or away from the opening (71).

10. The gene sequencing device according to claim 9, characterized in that: The box body (70) is provided with a safety protection member (74), and the safety protection member (74) is used to detect whether the opening (71) is blocked.

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