Collecting and separating device for stem cell exosome
By adopting cylinder-driven synchronous rotation and micro-water pump control in the stem cell exosome separation device, the problem of exosome exudation in the prior art is solved, and the purity and efficiency of the collected components are improved.
Patent Information
- Application Number
- CN202420906737.9
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-04-28
- Publication Date
- 2024-12-20
- Estimated Expiration
- 2034-04-28
AI Technical Summary
During the centrifugation process of the existing stem cell exosome separation device, exosomes with small diameters are prone to seep out, resulting in high impurities in the collection component, affecting the collection effect.
A device including a base, a scaffold, a centrifugal assembly and a collection assembly was designed. The cylinder drives the collection cylinder to lift and lower, so that the centrifugal assembly and the collection assembly rotate simultaneously, and combine it with a micro water pump and a control valve to realize the stem cell exosomes in the test tube. Collect separately to reduce impurity content.
It effectively avoids exudation of exosomes during centrifugation, improves the purity and collection efficiency of the collected components, and reduces the impurity content.
Smart Images
Figure CN222205238U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of medical devices, in particular to a collection and separation device for stem cell exosomes. Background Art
[0002] Exosomes are membrane vesicles with a diameter of about 30-150nm that are formed by the inward indentation of the cell membrane through endocytosis. After the multivesicular endosomes fuse with the cell membrane, they are released into the extracellular matrix. Exosomes are responsible for transmission and are an important medium for transmitting information between cells. In recent years, great progress has been made in the research on stem cell exosomes at home and abroad. Exosomes have strong repair, regeneration and protection effects in the cardiovascular system, traumatic brain injury, musculoskeletal injury, liver injury, kidney injury, etc. Stem cell exosomes are the best choice and a new type of repair method that can achieve cell regeneration.
[0003] A device for separating and collecting stem cell exosomes was published in Chinese patent publication number CN214937370U. The device can place separated plasma into a placement plate. Due to the drainage effect of the guide groove and the guide hole, the separated plasma is transported to the collection box through the guide tube, making it convenient for medical staff to collect the secretions of the separated stem cells.
[0004] However, after centrifugation, when the above structure is separated through the first through hole and the second through hole, exosomes with small diameters are easy to seep out from the first through hole and the second through hole, which easily leads to a high content of impurities in the collected components and affects the collection effect. Utility Model Content
[0005] The utility model provides a collection and separation device for stem cell exosomes to solve the problems raised in the background technology.
[0006] To achieve the above-mentioned purpose, the utility model provides the following technical solutions: a stem cell exosome collection and separation device, comprising a base, a bracket installed on the outside of the base, and a centrifugal assembly, wherein the centrifugal assembly is arranged on the upper surface of the base, and a test tube is detachably installed on the centrifugal assembly; a collection assembly, wherein the collection assembly is arranged on the inner side of the bracket and is used for collecting the separated stem cell exosomes in the test tube; the collection assembly comprises a cylinder fixed on the bracket, wherein a collection barrel is connected to a bearing at the telescopic end of the cylinder, and collection tubes corresponding to the test tubes are arranged on the outside of the collection barrel, and a straw that can extend into the test tube is installed at the lower end of the collection tube, and a connecting piece is provided at the lower end of the collection barrel for connecting the collection assembly and the centrifugal assembly so that the collection assembly and the centrifugal assembly rotate synchronously.
[0007] As a preferred technical solution of the utility model, the centrifuge assembly includes a turntable rotatably mounted on the upper surface of the base, a central axis is fixed at the center of the turntable, a mounting frame is detachably mounted on the upper end of the central axis, and placement slots for test tube installation are evenly spaced on the mounting frame; a fixed slot adapted to the central axis is opened in the middle of the mounting frame, and a second limit plate abutting against the lower surface of the mounting frame is fixed at the outer side of the central axis near the upper end; mounting slots are evenly spaced on the mounting frame, and a positioning slot is opened on the outer edge of the mounting frame.
[0008] As a preferred technical solution of the utility model, the connecting part includes a telescopic rod fixed to the middle part of the lower surface of the collection tube, a first limit plate is fixed to the lower end of the telescopic rod, and a top cover is slidably installed on the telescopic rod, a sliding hole adapted to the telescopic rod is opened in the middle of the top cover, and a spring is installed on the outside of the telescopic rod between the sliding hole and the collection tube; through holes are opened on the top cover at equal intervals, and the collection tube passes through the through holes and is slidably connected thereto; a positioning plate is fixed to the lower end of the outer side of the top cover, and the positioning plate is adapted to the positioning groove, and limiting columns are symmetrically fixed to the lower surface of the top cover, and the lower ends of the limiting columns can be inserted into the installation through grooves.
[0009] As a preferred technical solution of the utility model, limit strips are symmetrically fixed on both sides of the central axis, and limit slots adapted to the limit strips are opened on the fixed through slots.
[0010] As a preferred technical solution of the utility model, a micro water pump connected to the collection pipe is installed inside the collection tube, and an output pipe is installed at the lower end of the outer side of the collection tube, and a control valve is provided on the output pipe.
[0011] As a preferred technical solution of the utility model, the central shaft is a component made of stainless steel, a magnet is fixed to the lower end of the first limiting plate, and the magnet is magnetically attracted to the central shaft.
[0012] Compared with the prior art, the utility model provides a collection and separation device for stem cell exosomes, which has the following beneficial effects:
[0013] The stem cell exosome collection and separation device drives the collection tube to rise and fall by a cylinder so that the connecting piece contacts the centrifugal assembly, thereby allowing the centrifugal assembly to drive the collection assembly to rotate synchronously, thereby avoiding affecting the collection operation of the collection tube. After the cells are broken, sedimentation and liquid separation are performed, and then the test tube is collected separately through the collection tube, which is beneficial to the collection of stem cell exosomes inside the test tube and reduces the impurity content after collection. BRIEF DESCRIPTION OF THE DRAWINGS
[0014] Figure 1 It is a schematic diagram of the structure of the utility model;
[0015] Figure 2This is a schematic diagram of the structure of the top cover of the utility model when it is moving upward;
[0016] Figure 3 For the utility model Figure 2 Another perspective of the picture;
[0017] Figure 4 This is a schematic diagram of the collection structure of the test tube in the utility model;
[0018] Figure 5 For this utility model Figure 4 Schematic diagram of the internal structure.
[0019] In the figure: 1. base; 2. bracket; 3. cylinder; 4. turntable; 5. central axis; 6. mounting frame; 7. placement slot; 8. test tube; 9. collection tube; 10. telescopic rod; 11. spring; 12. output tube; 13. top cover; 14. collection tube; 15. installation slot; 16. positioning slot; 17. positioning plate; 18. sliding hole; 19. fixed slot; 20. first limit plate; 21. limit column; 22. straw; 23. second limit plate; 24. micro water pump. DETAILED DESCRIPTION
[0020] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.
[0021] See also Figure 1-Figure 5 The utility model discloses a collection and separation device for stem cell exosomes, including a base 1, a bracket 2 installed on the outside of the base 1, and a centrifugal component. The centrifugal component is arranged on the upper surface of the base 1, and a test tube 8 is detachably installed on the centrifugal component. Through the arrangement of the centrifugal component, the plasma containing stem cells in the test tube 8 is subjected to centrifugal action, which is conducive to the breakage of plasma cells and the release of stem cell exosomes. In addition, in order to facilitate the collection of stem cell exosomes, this embodiment also includes a collection component, which is arranged on the inner side of the bracket 2 and is used for collecting the stem cell exosomes in the test tube 8 after separation.
[0022] The above-mentioned collection component includes a cylinder 3 fixed on the bracket 2, and the telescopic end bearing of the cylinder 3 is connected to a collection tube 9. A collection tube 14 corresponding to the test tube 8 is provided on the outside of the collection tube 9, and a suction tube 22 that can extend into the test tube 8 is installed at the lower end of the collection tube 14; a connecting piece is provided at the lower end of the collection tube 9 for connecting the collection component and the centrifugal component so that the collection component and the centrifugal component rotate synchronously.
[0023] The cylinder 3 drives the collection tube 9 to rise and fall so that the connecting piece contacts the centrifugal assembly, thereby allowing the centrifugal assembly to drive the collection assembly to rotate synchronously, avoiding affecting the collection operation of the collection tube 14. After the cells are broken, sedimentation and liquid separation are performed, and then the test tube 8 is collected separately through the collection tube 14, which is beneficial to the collection of stem cell exosomes inside the test tube 8 and reduces the impurity content after collection.
[0024] like Figure 1 , Figure 2 , Figure 4 and Figure 5 As shown, the centrifuge assembly provided in this embodiment includes a turntable 4 rotatably mounted on the upper surface of the base 1, a central axis 5 is fixed at the center of the turntable 4, a mounting frame 6 is detachably mounted on the upper end of the central axis 5, and placement slots 7 for installing test tubes 8 are evenly spaced on the mounting frame 6. When the turntable 4 rotates, the test tubes 8 are driven to rotate, so that the test tubes 8 are subjected to centrifugal action, and further the plasma containing stem cells in the test tubes 8 are subjected to centrifugal action, which is beneficial to the breakage of plasma cells and the release of stem cell exosomes.
[0025] In addition, in order to facilitate the installation of the mounting frame 6, a fixed through groove 19 adapted to the central axis 5 is opened in the middle of the mounting frame 6, and a second limiting plate 23 that abuts against the lower surface of the mounting frame 6 is fixed to the outer side of the central axis 5 near the upper end. The fixing through groove 19 and the central axis 5 are plugged in, and the lower surface of the mounting frame 6 is limited by the second limiting plate 23, thereby achieving the fixation of the mounting frame 6.
[0026] Furthermore, in order to facilitate the positioning of the mounting frame 6 , mounting through grooves 15 are provided at equal intervals on the mounting frame 6 , and a positioning groove 16 is provided on the outer edge of the mounting frame 6 .
[0027] like Figure 3-Figure 5 As shown, the above-mentioned connecting piece includes a telescopic rod 10 fixed to the middle part of the lower surface of the collection tube 9, and the telescopic rod 10 is composed of a sleeve and an insertion rod. When the top cover 13 is separated from the test tube 8, the insertion rod will extend from the inside of the sleeve due to the gravity of the top cover 13. At this time, the telescopic rod 10 is at its longest. When the top cover 13 is against the test tube 8, the collection tube 9 can continue to descend to facilitate the subsequent extraction after liquid separation. A first limit plate 20 is fixed to the lower end of the telescopic rod 10, and a top cover 13 is slidably installed on the telescopic rod 10. A sliding hole 18 adapted to the telescopic rod 10 is opened in the middle of the top cover 13. A spring 11 is installed on the outside of the telescopic rod 10 between the sliding hole 18 and the collection tube 9. During the descent of the collection tube 9, the telescopic rod 10 is against the central axis 5. Under the elastic force of the spring 11, the top cover 13 covers the upper end of the test tube 8 to prevent the plasma inside the test tube 8 from overflowing during centrifugation.
[0028] In order to facilitate the movement of the top cover 13, through holes are opened on the top cover 13 at equal intervals, and the collection tube 14 passes through the through holes and is slidably connected thereto.
[0029] In addition, in order to facilitate the positioning of the mounting frame 6 when it is connected to the top cover 13, a positioning plate 17 is fixed to the lower end of the outer side of the top cover 13, and the positioning plate 17 is adapted to the positioning groove 16. Through the cooperation between the positioning plate 17 and the positioning groove 16, the top cover 13 can cover the test tube 8. At this time, the pipette 22 is inserted into the test tube 8. By controlling the height of the collection tube 9, the depth of the pipette 22 inserted into the test tube 8 is controlled, so as to facilitate extraction and collection according to the actual liquid separation situation. The limiting column 21 is symmetrically fixed on the lower surface of the top cover 13, and the lower end of the limiting column 21 can be inserted into the mounting groove 15. Through the cooperation between the limiting column 21 and the mounting groove 15, the mounting frame 6 can drive the top cover 13 to rotate.
[0030] like Figure 2 As shown, in order to further improve the stability of the mounting frame 6 during installation, limit strips are symmetrically fixed on both sides of the central axis 5, and limit grooves adapted to the limit strips are opened on the fixed through grooves 19 to improve the coordination between the limit grooves and the limit strips and improve the stability of the mounting frame 6.
[0031] like Figure 5 As shown, in order to facilitate the collection of stem cell exosomes, a micro water pump 24 connected to the collection tube 14 is installed inside the collection tube 9, and an output tube 12 is installed at the lower end of the outer side of the collection tube 9. A control valve is provided on the output tube 12. The stem cell exosomes are sucked into the collection tube 9 through the cooperation of the micro water pump 24 and the collection tube 14, and the output of the stem cell exosomes is controlled by the output tube 12 and the control valve.
[0032] like Figure 5 As shown, in order to improve the stability of the connection between the central shaft 5 and the first limiting plate 20 , the central shaft 5 is a component of stainless steel, and a magnet is fixed at the lower end of the first limiting plate 20 , and the magnet is magnetically attracted to the central shaft 5 .
[0033] The working principle and use process of the utility model are as follows: when in use, the plasma containing stem cells is placed inside the test tube 8, the test tube 8 is installed inside the placement slot 7, and then the mounting frame 6 is installed on the central axis 5, and the cylinder 3 is started so that the collection tube 9 drives the top cover 13 to cover the top of the mounting frame 6. During this process, the top cover 13 is rotated so that the positioning plate 17 is aligned with the positioning slot 16. At this time, the limit column 21 below the top cover 13 is inserted into the mounting slot 15, so that the mounting frame 6 drives the top cover 13 to rotate. At this time, the upper end of the test tube 8 is in a closed state, which can prevent the plasma from overflowing during the centrifugation process. After the centrifugation is completed, the test tube 8 is left to stand. After the solution in the test tube 8 is separated, the cylinder 3 is continued to be started to control the collection tube 9 to move downward. At this time, the telescopic rod 10 is contracted, the spring 11 is compressed, and the top cover 13 is fixed above the central axis 5. By the downward movement of the collection tube 9, the collection tube 14 can be moved downward, and then the suction tube 22 is controlled to extend into the test tube 8. The depth of the internal depth, so as to collect stem cell exosomes according to the actual separation situation.
[0034] It should be noted that, in this article, terms such as "comprises", "includes" or any other variations thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also includes other elements not explicitly listed, or also includes elements inherent to such process, method, article or device. In the absence of more restrictions, an element defined by the sentence "comprises a ..." does not exclude the existence of other identical elements in the process, method, article or device including the element.
[0035] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.
Claims
1. A device for collecting and separating stem cell exosomes, comprising a base (1) and a support (2) installed outside the base (1), characterized in that: Also includes: A centrifugal assembly, the centrifugal assembly being arranged on the upper surface of the base (1), and a test tube (8) being detachably mounted on the centrifugal assembly; A collection component, which is arranged on the inner side of the support (2) and is used for collecting the separated stem cell exosomes in the test tube (8); The collection assembly comprises a cylinder (3) fixed on a support (2); a collection tube (9) is connected to a bearing at the telescopic end of the cylinder (3); a collection tube (14) corresponding to the test tube (8) is arranged on the outside of the collection tube (9); a suction tube (22) which can extend into the test tube (8) is installed at the lower end of the collection tube (14); The lower end of the collecting tube (9) is provided with a connecting piece for connecting the collecting assembly and the centrifugal assembly so that the collecting assembly and the centrifugal assembly rotate synchronously.
2. The stem cell exosome collection and separation device according to claim 1, characterized in that: The centrifuge assembly comprises a turntable (4) rotatably mounted on the upper surface of a base (1), a central axis (5) being fixed at the center of the turntable (4), a mounting frame (6) being detachably mounted on the upper end of the central axis (5), and placement slots (7) for mounting test tubes (8) being provided at equal intervals on the mounting frame (6); A fixed through slot (19) adapted to the central axis (5) is provided in the middle of the mounting frame (6), and a second limiting plate (23) abutting against the lower surface of the mounting frame (6) is fixed on the outer side of the central axis (5) near the upper end; The mounting frame (6) is provided with mounting through slots (15) at equal intervals, and a positioning slot (16) is provided on the outer edge of the mounting frame (6).
3. The stem cell exosome collection and separation device according to claim 2, characterized in that: The connecting member comprises a telescopic rod (10) fixed to the middle part of the lower surface of the collection tube (9), a first limit plate (20) is fixed to the lower end of the telescopic rod (10), and a top cover (13) is slidably mounted on the telescopic rod (10), a sliding hole (18) adapted for the telescopic rod (10) is opened in the middle part of the top cover (13), and a spring (11) is mounted on the outer side of the telescopic rod (10) between the sliding hole (18) and the collection tube (9); The top cover (13) is provided with through holes at equal intervals, and the collection tube (14) passes through the through holes and is slidably connected thereto; A positioning plate (17) is fixed to the lower end of the outer side of the top cover (13), and the positioning plate (17) is adapted to the positioning groove (16). A limiting column (21) is symmetrically fixed to the lower surface of the top cover (13), and the lower end of the limiting column (21) can be inserted into the installation through groove (15).
4. The stem cell exosome collection and separation device according to claim 3, characterized in that: Limiting strips are symmetrically fixed on both sides of the central axis (5), and limiting grooves adapted to the limiting strips are provided on the fixed through groove (19).
5. The stem cell exosome collection and separation device according to any one of claims 1 to 4, characterized in that: A micro water pump (24) connected to the collection tube (14) is installed inside the collection tube (9), and an output tube (12) is installed at the lower end of the outer side of the collection tube (9), and a control valve is provided on the output tube (12).
6. The stem cell exosome collection and separation device according to claim 3, characterized in that: The central shaft (5) is a component made of stainless steel, and a magnet is fixed to the lower end of the first limiting plate (20), and the magnet is magnetically attracted to the central shaft (5).