Isolation magnetic bead type suction head

Abstract

An isolation magnetic bead type suction head comprises a plug arranged at the upper end of a test tube, the plug is communicated with a plurality of communicating holes, communicating heads are arranged at the upper ends of the communicating holes, and when the communicating heads are used for suction, the inside and the outside of the pointed test tube can be under the same pressure, so that the suction operation of an extracting solution is facilitated, and impurities can be prevented from entering the extracting solution. According to the utility model, the extraction liquid can be conveniently sucked, the extraction liquid can be prevented from being polluted, and meanwhile, magnetic beads can be prevented from being sucked out.

Description

Technical Field

[0001] The utility model relates to the technical field of DNA extraction, in particular to an isolated magnetic bead type suction head. Background Art

[0002] With the development of biological DNA extraction methods, it is now popular to use more efficient and environmentally friendly magnetic bead kits to replace the previous phenol-chloroform extraction method. The magnetic bead kit is also suitable for high-throughput nucleic acid extraction instruments, which is irreplaceable by traditional methods.

[0003] During extraction, the magnetic beads are usually located at the bottom of the pointed test tube by magnetic attraction, and then the obtained extract is extracted by a pipette. During the suction process, foreign impurities are easily introduced into the extract, thereby contaminating the extract. Furthermore, the magnetic beads are easily introduced into the suction tube, thereby affecting subsequent detection operations. Utility Model Content

[0004] The utility model provides an isolated magnetic bead type suction head to solve the above-mentioned deficiencies of the prior art, can avoid the pollution of the extraction liquid by the outside world, and prevents the magnetic beads from being extracted, and has strong practicality.

[0005] In order to achieve the purpose of this utility model, the following technologies are proposed:

[0006] An isolated magnetic bead type suction head includes a plug arranged at the upper end of a test tube, a plurality of connecting holes are connected on the plug, and a connecting head is arranged at the upper end of the connecting holes. When the connecting head is used for suction, the inside and the outside of the pointed test tube can be at the same pressure, thereby facilitating the suction operation of the extract and preventing impurities from entering the extract.

[0007] An inner extension tube is passed through the plug, and an isolation disk is provided at the inner end of the inner extension tube. A lower suction tube is provided at the lower end of the isolation disk, and a conical part is provided at the upper end of the isolation disk. The upper end of the conical part is a small end, and a plurality of first holes are provided on the conical wall of the conical part. A second hole is provided on the isolation disk, and a third hole is provided on the lower suction tube. An upper conical tube is provided at the upper end of the inner extension tube, and an upper tube is provided at the upper end of the upper conical tube. A connecting tube head is connected to the upper end of the upper conical tube, and the lower end of the upper conical tube is a small end. A ball is provided in the upper conical tube, and a spring is provided at the upper end of the ball. The upper end of the spring is provided inside the upper end of the upper tube. When performing operations such as connection, the ball can close the inner extension tube under the action of the spring to prevent impurities from entering. During the suction process, the isolation disk can block the upward movement of the magnetic beads, thereby preventing the magnetic beads from being sucked out, and the second hole opened on it is smaller than the diameter of the magnetic beads. Even if some magnetic beads are located above the isolation disk after magnetic suction, the first hole can prevent the magnetic beads from entering. The setting of the conical part expands the liquid inlet area and prevents the magnetic beads from blocking the first hole. The lower pipette extends into the interior of the pointed test tube, where a large number of magnetic beads exist due to magnetic suction, and the third hole opened on it facilitates the suction of the extract therein.

[0008] Further, the connecting head includes a connecting pipe arranged at the upper end of the connecting hole, an air inlet cover is arranged at the upper end of the connecting pipe, a connecting end pipe is arranged at the upper end of the air inlet cover, an inner cover is arranged at the inner side end of the connecting end pipe, air holes are respectively provided on the inner cover and the air inlet cover, a connecting inner pipe is provided at the inner side end of the inner cover, an end plate is arranged at the inner side end of the connecting inner pipe, a plurality of air holes are provided on the end plate, and the inner cover is filled with activated carbon. The activated carbon arranged inside the inner cover facilitates filtering of the incoming air to prevent impurities from entering the test tube, and this design method also facilitates the replacement of the inner cover.

[0009] Furthermore, an end cap is provided at the outer end of the connecting end pipe to facilitate operations such as replacement of the inner cover.

[0010] Furthermore, the lower end of the lower suction pipe is a conical structure, and the lower end is a small end, so as to facilitate the insertion operation of the lower suction pipe.

[0011] Furthermore, the axial direction of the first hole and the conical surface of the conical member are perpendicular to each other, which can prevent the magnetic beads from entering the first hole.

[0012] Furthermore, the outer periphery of the isolation disk is in a conical structure, and the lower end thereof is a small end so as to limit the lower end of the inner extension tube.

[0013] The advantages of the above technical solution are:

[0014] The utility model is convenient for sucking the extracting liquid, can prevent the extracting liquid from being contaminated, and can also prevent the magnetic beads from being sucked out. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] In order to make the purpose, technical solutions and advantages of the utility model clearer, the utility model will be further described in detail below with reference to the accompanying drawings.

[0016] Figure 1 A three-dimensional structural diagram of one embodiment is shown.

[0017] Figure 2 A cross-sectional view of one embodiment is shown.

[0018] Figure 3 An enlarged view of A is shown.

[0019] Figure 4 An enlarged view of B is shown. DETAILED DESCRIPTION

[0020] like Figure 1~Figure 4 As shown, an isolated magnetic bead type suction head includes a plug 10 arranged at the upper end of a test tube 1, a plurality of connecting holes 11 are connected on the plug 10, and a connecting head is arranged at the upper end of the connecting hole 11. The connecting head includes a connecting tube 2 arranged at the upper end of the connecting hole 11, an air inlet cover 20 is arranged at the upper end of the connecting tube 2, a connecting end tube 21 is arranged at the upper end of the air inlet cover 20, and an end cap is arranged at the outer end of the connecting end tube 21. An inner cover 22 is arranged at the inner side end of the connecting end tube 21, and air holes are respectively opened on the inner cover 22 and the air inlet cover 20, and a connecting inner tube 23 is arranged at the inner side end of the inner cover 22, and an end plate is arranged at the inner side end of the connecting inner tube 23, and a plurality of air holes are opened on the end plate, and the inner cover 22 is filled with activated carbon.

[0021] The plug 10 is penetrated by an inner extension tube 3, and the inner end of the inner extension tube 3 is an isolation disk 37, the outer periphery of which is a conical structure, and its lower end is a small end. The lower end of the isolation disk 37 is provided with a lower suction pipe 39, and the lower end of the lower suction pipe 39 is a conical structure, and the lower end is a small end. The upper end of the isolation disk 37 is provided with a conical member 35, and the upper end of the conical member 35 is a small end. A plurality of first holes 36 are opened on the conical wall of the conical member 35, and the axial direction of the first hole 36 is perpendicular to the conical surface of the conical member 35. A second hole 38 is provided on the isolation disk 37, a third hole is provided on the lower suction pipe 39, an upper conical tube 30 is provided at the upper end of the inner extension tube 3, an upper tube 31 is provided at the upper end of the upper conical tube 30, a connecting tube head 34 is connected to the upper end of the upper conical tube 30, the lower end of the upper conical tube 30 is a small end, a ball 32 is provided in the upper conical tube 30, a spring 33 is provided at the upper end of the ball 32, and the upper end of the spring 33 is provided inside the upper end of the upper tube 31.

[0022] In this embodiment, when the inner cover 22 is replaced, the inner cover 22 that has been used for a long time is taken out, and then a new inner cover 22 is screwed onto the upper end of the air intake cover 20.

[0023] When performing the suction operation in this embodiment, the pointed test tube 1 is first placed on the permanent magnetic table, and the magnetic beads are adsorbed by the permanent magnetic table. The magnetic beads are located at the lower end of the pointed test tube 1 through the adsorption, and then the inner extension tube 3 is passed through the test tube, and the plug 10 is plugged at the upper end of the test tube 1, and then the suction tube of the pipette is connected to the connecting tube head 34, and then the pipette is started, and the extraction liquid located at the lower end of the test tube 1 enters the lower suction tube 39 through the third hole through the suction of the pipette, and the extraction liquid located at the upper part of the test tube 1 will enter the inner extension tube 3 through the first hole 36, and in the process of suction, the ball 32 moves upward from the upper conical tube 30, and then under the action of suction, the extraction liquid enters the upper conical tube 30 and finally enters the pipette, and in the process of suction, the outside air will enter the inner cover 22 through the air inlet cover 20, and the impurities therein are filtered by the activated carbon, and then enter the inside of the test tube 1 through the air vent, so as to facilitate the extraction operation of the extraction liquid.

[0024] The above description is only a preferred embodiment of the utility model and is not intended to limit the utility model. Obviously, those skilled in the art can make various changes and modifications to the utility model without departing from the spirit and scope of the utility model. Thus, if these modifications and variations of the utility model fall within the scope of the claims of the utility model and their equivalents, the utility model is also intended to include these modifications and variations.

Claims

1. An isolated magnetic bead type suction head, characterized in that: It comprises a plug (10) arranged at the upper end of the test tube (1), a plurality of communication holes (11) being connected to the plug (10), and a communication head being arranged at the upper end of the communication hole (11); The plug (10) is provided with an inner extension tube (3), an isolation disk (37) at the inner side end of the inner extension tube (3), a lower suction tube (39) at the lower end of the isolation disk (37), a conical member (35) at the upper end of the isolation disk (37), a plurality of first holes (36) are provided on the conical wall of the conical member (35), a second hole (38) is provided on the isolation disk (37), a third hole is provided on the lower suction tube (39), an upper conical tube (30) is provided at the upper end of the inner extension tube (3), an upper tube (31) is provided at the upper end of the upper conical tube (30), a connecting tube head (34) is connected at the upper end of the upper conical tube (30), a ball (32) is provided in the upper conical tube (30), a spring (33) is provided at the upper end of the ball (32), and the upper end of the spring (33) is arranged inside the upper end of the upper tube (31).

2. The isolated magnetic bead type suction head according to claim 1, characterized in that: The connecting head comprises a connecting pipe (2) arranged at the upper end of the connecting hole (11); an air inlet cover (20) is arranged at the upper end of the connecting pipe (2); a connecting end pipe (21) is arranged at the upper end of the air inlet cover (20); an inner cover (22) is arranged at the inner side end of the connecting end pipe (21); air holes are respectively provided on the inner cover (22) and the air inlet cover (20); a connecting inner pipe (23) is provided at the inner side end of the inner cover (22); an end plate is arranged at the inner side end of the connecting inner pipe (23); a plurality of air holes are provided on the end plate; and activated carbon is filled in the inner cover (22).

3. The isolated magnetic bead type suction head according to claim 2, characterized in that: An end cap is provided at the outer end of the connecting end pipe (21).

4. The isolated magnetic bead type suction head according to claim 1, characterized in that: The lower end of the lower suction pipe (39) is a conical structure, and the lower end is a small end.

5. The isolated magnetic bead type suction head according to claim 1, characterized in that: The axial direction of the first hole (36) and the conical surface of the conical member (35) are perpendicular to each other.

6. The isolated magnetic bead type suction head according to claim 1, characterized in that: The outer circumference of the isolation disk (37) is in a conical structure, and the lower end thereof is a small end.

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