Portable nucleic acid detector and nucleic acid detection device

Abstract

The utility model relates to a portable nucleic acid detector and a nucleic acid detection device. The portable nucleic acid detector comprises a column body, a filter element, a sealing film, a first puncture piece, a second puncture piece and a third puncture piece, and the column body is provided with a first containing channel, a second containing channel, a third containing channel and an air pressure balance channel which penetrate through the column body. The same end face of the column body is provided with a first opening communicated with the first containing channel, a second opening communicated with the second containing channel, a third opening communicated with the third containing channel and a fourth opening communicated with the air pressure balance channel, and the column body is connected with a sealing film used for sealing the first opening, the second opening and the third opening. The puncturing piece can puncture the sealing film, and the column body can be connected with the sample reaction tube in a sealing manner. The portable nucleic acid detector and the nucleic acid detection device are low in cost, convenient to carry, easy to operate, rapid in detection and convenient to read.

Description

Technical Field

[0001] The present application relates to the technical field of nucleic acid detection, and in particular to a portable nucleic acid detector and a nucleic acid detection device. Background Art

[0002] Nucleic acid testing can be used to detect viruses quickly and efficiently, thereby timely discovering and preventing virus transmission. Individualized decentralized testing can greatly reduce the risk of transmission caused by gatherings of people, reduce the pressure on infectious disease prevention and control in large hospitals, and prevent the risk of cross-contamination in large-scale diagnostic units.

[0003] At present, nucleic acid testing instruments are generally bulky, inconvenient to carry and use. In addition, nucleic acid testing usually requires professional laboratory conditions and operators, with high testing costs and cumbersome operations, which cannot meet the needs of individualized nucleic acid testing. Utility Model Content

[0004] Based on this, one or more embodiments of the present application provide a portable nucleic acid detector and a nucleic acid detection device.

[0005] The technical solution of this application includes the following contents:

[0006] A portable nucleic acid detector comprises a column, a filter element, a sealing membrane, a first puncture member, a second puncture member and a third puncture member;

[0007] The column has a first accommodating channel, a second accommodating channel, a third accommodating channel and an air pressure balance channel running through the column, the first accommodating channel is used to accommodate an amplification reagent for nucleic acid detection, the second accommodating channel is used to accommodate a test strip for nucleic acid detection, the third accommodating channel is used to accommodate a diluent for nucleic acid detection, and the filter element is provided in the air pressure balance channel;

[0008] The same end surface of the column has a first opening connected to the first accommodating channel, a second opening connected to the second accommodating channel, a third opening connected to the third accommodating channel, and a fourth opening connected to the air pressure balance channel, and the column is connected to the sealing film for sealing the first opening, the second opening, and the third opening;

[0009] The column can be sealed and connected to the sample reaction tube;

[0010] The first accommodating channel is provided with a movable first puncture member, the first puncture member can be dynamically sealed with the inner wall of the first accommodating channel, and the first puncture member can pierce the sealing film; the second accommodating channel is provided with a movable second puncture member, the second puncture member is connected to the test strip, the second puncture member can be dynamically sealed with the inner wall of the second accommodating channel, and the second puncture member can pierce the sealing film; the third accommodating channel is provided with a movable third puncture member, the third puncture member can be dynamically sealed with the inner wall of the third accommodating channel, and the third puncture member can pierce the sealing film.

[0011] In one embodiment, the first piercing member includes a first sealing portion and a first piercing portion connected to each other, and the first sealing portion is dynamically sealed with the inner wall of the first accommodating channel;

[0012] An end of the first sealing portion away from the first puncture portion protrudes from the column to provide a force application site.

[0013] In one embodiment, a radial dimension of the first sealing portion is larger than a dimension of the first opening, so that the first sealing portion is confined within the first accommodating channel.

[0014] In one embodiment, the second piercing member includes a second sealing portion and a second piercing portion connected to each other, and the second sealing portion is dynamically sealed with the inner wall of the second accommodating channel;

[0015] An end of the second sealing portion away from the second puncture portion protrudes from the column to provide a force application point.

[0016] In one embodiment, a radial dimension of the second sealing portion is larger than a dimension of the second opening, so that the second sealing portion is confined within the second accommodating channel.

[0017] In one embodiment, a portion of the test strip is embedded in the second sealing portion, and another portion of the test strip extends to the second puncture portion.

[0018] In one embodiment, the third puncture member includes a third sealing portion and a third puncture portion connected to each other, and the third sealing portion is dynamically sealed with the inner wall of the third accommodating channel;

[0019] An end of the third sealing portion away from the third puncture portion protrudes from the column to provide a force application point.

[0020] In one embodiment, a radial dimension of the third sealing portion is greater than a dimension of the third opening, so that the third sealing portion is confined within the third accommodating channel.

[0021] In one embodiment, the column further includes a plug-in connector, which enables the column and the sample reaction tube to be sealed and connected by plugging.

[0022] A portable nucleic acid detection device, comprising:

[0023] A nucleic acid extraction tube, which is used to extract nucleic acid to be tested from a biological sample;

[0024] a sample reaction tube containing a solid amplification reagent for amplifying the nucleic acid to be tested; and

[0025] The portable nucleic acid detector described in any of the above technical solutions.

[0026] By using the portable nucleic acid detector and nucleic acid detection device of the present application, the amplification reagent, test strip and diluent used for nucleic acid detection can be pre-stored in different containing channels respectively, which saves reagent storage space and eliminates the time for liquid preparation, and is convenient for taking and using. When testing is needed, the portable nucleic acid detector and the sample reaction tube are sealed and connected, and then different piercing members are controlled to pierce the sealing membranes of different containing channels in turn, so that the amplification reagent used for nucleic acid detection enters the sample reaction tube for reaction, the diluent enters the sample reaction tube for dilution, and the test strip sample reaction tube is used for detection. The whole process does not require the addition of other reagents. It is simple to operate, easy to use, and easy to read. It has the advantages of being portable, simple, and intuitive, and is suitable for individualized nucleic acid detection.

[0027] By using the portable nucleic acid detector of the present application, the nucleic acid detection process can be carried out under relatively sealed conditions without causing nucleic acid leakage, thus having higher safety.

[0028] It should be understood that the content described in this section is not intended to identify the key or important features of the embodiments of the present disclosure, nor is it intended to limit the scope of the present disclosure. Other features of the present disclosure will become easily understood through the following description. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] By reading the detailed description below with reference to the accompanying drawings, the above and other purposes, features and advantages of the exemplary embodiments of the present application will become readily understood. In the accompanying drawings, several embodiments of the present application are shown in an exemplary and non-limiting manner, wherein:

[0030] In the drawings, the same or corresponding reference numerals represent the same or corresponding parts.

[0031] Figure 1 The structure of the portable nucleic acid detector in the embodiment of the present application is shown Figure 1 .

[0032] Figure 2The structure of the portable nucleic acid detector in the embodiment of the present application is shown Figure 2 .

[0033] Figure 3 The structure of the portable nucleic acid detector in the embodiment of the present application is shown Figure 3 .

[0034] Figure 4 The structure of the portable nucleic acid detector in the embodiment of the present application is shown Figure 4 .

[0035] Figure 5 The structure of the portable nucleic acid detector in the embodiment of the present application is shown Figure 5 .

[0036] Figure 6 The structure of the nucleic acid detection device of the embodiment of the present application is shown Figure 1 .

[0037] Figure 7 The structure of the nucleic acid detection device of the embodiment of the present application is shown Figure 2 .

[0038] Figure 8 The structure of the nucleic acid detection device of the embodiment of the present application is shown Figure 3 .

[0039] Explanation of the accompanying drawings: 1-portable nucleic acid detector; 2-nucleic acid extraction tube; 3-sample reaction tube; 10-column; 11-first accommodating channel; 12-second accommodating channel; 13-third accommodating channel; 14-air pressure balance channel; 20-filter element; 30-sealing membrane; 41-first piercing member; 42-second piercing member; 43-third piercing member; 112-first opening; 122-second opening; 132-third opening; 142-fourth opening; 111-fifth opening; 121-sixth opening; 131-seventh opening; 141-eighth opening. DETAILED DESCRIPTION

[0040] The above-mentioned purpose, features and advantages of the present application can be more clearly understood, and the specific implementation methods of the present application are described in detail below in conjunction with the accompanying drawings. Many specific details are set forth in the following description to facilitate a full understanding of the present application. However, the present application can be implemented in many other ways different from those described herein, and those skilled in the art can make similar improvements without violating the connotation of the present application, so the present application is not limited by the specific embodiments disclosed below.

[0041] The first aspect of the present application provides a portable nucleic acid detector.

[0042] Figure 1The structure of the portable nucleic acid detector in the embodiment of the present application is shown Figure 1 ; Figure 2 The structure of the portable nucleic acid detector in the embodiment of the present application is shown Figure 2 ; Figure 3 The structure of the portable nucleic acid detector in the embodiment of the present application is shown Figure 3 ; Figure 4 The structure of the portable nucleic acid detector in the embodiment of the present application is shown Figure 4 ; Figure 5 The structure of the portable nucleic acid detector in the embodiment of the present application is shown Figure 5 ; Please refer to Figure 1 , Figure 2 , Figure 3 , Figure 4 and Figure 5 .

[0043] like Figure 1 and Figure 2 As shown, a portable nucleic acid detector according to an embodiment of the present application includes a column 10, a filter element 20, a sealing membrane 30, a first puncture member 41, a second puncture member 42 and a third puncture member 43; the column 10 has a first accommodating channel 11, a second accommodating channel 12, a third accommodating channel 13 and an air pressure balance channel 14 running through the column 10, the first accommodating channel 11 is used to accommodate liquid reagents for nucleic acid amplification, the second accommodating channel 12 is used to accommodate test strips for nucleic acid detection, the third accommodating channel 13 is used to accommodate diluent, a filter element 20 is arranged in the air pressure balance channel 14, one end face of the column 10 has a first opening 112 connected to the first accommodating channel 11, a second opening 122 connected to the second accommodating channel 12, and a third opening 132 connected to the third accommodating channel 13, the column 1 0 is connected to a sealing film 30 for sealing the first opening 112, the second opening 122 and the third opening 132, and the column 10 can be sealed with the sample reaction tube; a movable first puncture member 41 is provided in the first accommodating channel 11, and the first puncture member 41 can be dynamically sealed with the inner wall of the first accommodating channel 11, and the first puncture member can pierce the sealing film 30; a movable second puncture member 42 is provided in the second accommodating channel 12, and a test strip is connected to the second puncture member 42, and the second puncture member 42 can be dynamically sealed with the inner wall of the second accommodating channel 12, and the second puncture member can pierce the sealing film 30; a movable third puncture member 43 is provided in the third accommodating channel 13, and the third puncture member 43 can be dynamically sealed with the inner wall of the third accommodating channel 13, and the third puncture member 43 can pierce the sealing film 30.

[0044] It can be understood that the other end surface of the column 10 has a fifth opening 111 connected to the first accommodating channel 11 , a second opening 121 connected to the second accommodating channel 12 , and a third opening 131 connected to the third accommodating channel 13 .

[0045] It can be understood that one of the openings of the air pressure balance channel 14 (the fourth opening 141) is on the same end surface as the first opening 112, the second opening 122 and the third opening 132, and the other opening of the air pressure balance channel 14 (the eighth opening 141) is on the same end surface as the fifth opening 111, the sixth opening 121 connected to the second accommodating channel 12, the seventh opening 131 connected to the third accommodating channel 13 and the other opening of the air pressure balance channel 14.

[0046] Preferably, the column 10 is transparent, so as to facilitate observation of the test strip connected to the second puncture member 42 disposed in the second accommodating channel 12 .

[0047] Preferably, the filter element 20 is a material that can filter nucleic acids, so that the portable nucleic acid detector does not release aerosols containing nucleic acids to the outside during the detection process.

[0048] Preferably, the filter element 20 can be dynamically sealed with the inner wall of the air pressure balance channel 14 and also has a gas permeation function.

[0049] like Figure 1 , Figure 2 and Figure 3 As shown, the first puncture member 41 of the portable nucleic acid detector of one embodiment of the present application includes a first sealing portion and a first puncture portion connected to each other, the first sealing portion is dynamically sealed with the inner wall of the first accommodating channel 11, and the end of the first sealing portion away from the first puncture portion protrudes from the column 10 to provide a force application site.

[0050] Preferably, a radial dimension of the first sealing portion is larger than a dimension of the first opening, so that the first sealing portion is confined within the first accommodating channel.

[0051] like Figure 1 , Figure 2 and Figure 4 As shown, the second puncture member 42 of the portable nucleic acid detector of one embodiment of the present application includes a second sealing portion and a second puncture portion connected to each other, the second sealing portion is dynamically sealed with the inner wall of the second accommodating channel 12, a portion of the test strip is embedded in the second sealing portion, another portion of the test strip extends to the second puncture portion, and the end of the second sealing portion away from the first puncture portion protrudes from the column 10 to provide a force application site.

[0052] Preferably, a radial dimension of the second sealing portion is larger than a dimension of the second opening, so that the second sealing portion is confined within the second accommodating channel.

[0053] like Figure 1 , Figure 2 and Figure 5As shown, the third puncture member 43 of the portable nucleic acid detector of one embodiment of the present application includes a third sealing portion and a third puncture portion connected to each other, the third sealing portion is dynamically sealed with the inner wall of the third accommodating channel 13, and the end of the third sealing portion away from the third puncture portion protrudes from the column 10 to provide a force application site.

[0054] Preferably, a radial dimension of the third sealing portion is larger than a dimension of the third opening, so that the third sealing portion is confined within the third accommodating channel.

[0055] The second aspect of the present application provides a portable nucleic acid detection device.

[0056] Figure 6 The structure of the portable nucleic acid detection device of the present application is shown in FIG. Figure 1 ; Figure 7 The structure of the portable nucleic acid detection device of the present application is shown in FIG. Figure 2 ; Figure 8 The structure of the portable nucleic acid detection device of the present application is shown in FIG. Figure 3 ; Please refer to Figure 6 , Figure 7 and Figure 8 .

[0057] like Figure 6 , Figure 7 and Figure 8 As shown, a portable nucleic acid detection device according to an embodiment of the present application includes a portable nucleic acid detector 1, a nucleic acid extraction tube 2 and a sample reaction tube 3.

[0058] Preferably, the structure of the portable nucleic acid detector 1 refers to the portable nucleic acid detector described in any technical solution in the first aspect.

[0059] like Figure 7 As shown, the sample reaction tube 3 of the portable nucleic acid detection device of one embodiment of the present application is sealed and connected to the nucleic acid detector 1 at one end of the sealing film 30, and the sample reaction tube 3 is connected to the atmosphere only through the air pressure balance channel 14.

[0060] like Figure 8 As shown, the first piercing member 41, the second piercing member 42 and the third piercing member 43 of the nucleic acid detector 1 of the portable nucleic acid detection device of one embodiment of the present application respectively pierce the sealing film 30 and partially enter the sample reaction tube 3.

[0061] The technical features of the above-mentioned implementation modes and examples can be combined in any appropriate manner. To make the description concise, not all possible combinations of the technical features in the above-mentioned implementation modes and examples are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0062] The above-described embodiments only express several implementation methods of the present application, but they cannot be understood as limiting the scope of the patent application. It should be pointed out that, for those of ordinary skill in the art, without departing from the concept of the present application, several variations and improvements can also be made, which all belong to the protection scope of the present application. In addition, it should be understood that after reading the above-mentioned teaching content of the present application, those skilled in the art can make various changes or modifications to the present application, and the equivalent forms obtained also fall within the protection scope of the present application. It should also be understood that the technical solutions obtained by those skilled in the art on the basis of the technical solutions provided in the present application through logical analysis, reasoning or limited experiments are all within the protection scope of the claims attached to the present application. Therefore, the protection scope of the patent of the present application shall be based on the attached claims, and the description can be used to interpret the content of the claims.

Claims

1. A portable nucleic acid detector, characterized in that: It includes a column, a filter element, a sealing film, a first piercing member, a second piercing member and a third piercing member; The column has a first accommodating channel, a second accommodating channel, a third accommodating channel and an air pressure balance channel running through the column, the first accommodating channel is used to accommodate an amplification reagent for nucleic acid detection, the second accommodating channel is used to accommodate a test strip for nucleic acid detection, and the third accommodating channel is used to accommodate a diluent for nucleic acid detection. The air pressure balance channel is provided with the filter element, the same end surface of the column has a first opening connected to the first accommodating channel, a second opening connected to the second accommodating channel and a third opening connected to the third accommodating channel, and the column is connected to the sealing film for sealing the first opening, the second opening and the third opening; the column can be sealed and connected to the sample reaction tube; The first accommodating channel is provided with a movable first puncture member, the first puncture member can be dynamically sealed with the inner wall of the first accommodating channel, and the first puncture member can pierce the sealing film; the second accommodating channel is provided with a movable second puncture member, the second puncture member is connected to the test strip, the second puncture member can be dynamically sealed with the inner wall of the second accommodating channel, and the second puncture member can pierce the sealing film; the third accommodating channel is provided with a movable third puncture member, the third puncture member can be dynamically sealed with the inner wall of the third accommodating channel, and the third puncture member can pierce the sealing film.

2. The portable nucleic acid detector according to claim 1, characterized in that: The first puncture member comprises a first sealing portion and a first puncture portion connected to each other, and the first sealing portion is dynamically sealed with the inner wall of the first accommodating channel; An end of the first sealing portion away from the first puncture portion protrudes from the column to provide a force application site.

3. The portable nucleic acid detector according to claim 2, characterized in that: The radial dimension of the first sealing portion is greater than the dimension of the first opening, so that the first sealing portion is confined within the first accommodating channel.

4. The portable nucleic acid detector according to claim 1, characterized in that: The second puncture member comprises a second sealing portion and a second puncture portion connected to each other, and the second sealing portion is dynamically sealed with the inner wall of the second accommodating channel; An end of the second sealing portion away from the second puncture portion protrudes from the column to provide a force application point.

5. The portable nucleic acid detector according to claim 4, characterized in that: The radial dimension of the second sealing portion is greater than the dimension of the second opening, so that the second sealing portion is confined within the second accommodating channel.

6. The portable nucleic acid detector according to claim 4 or 5, characterized in that: A portion of the test strip is embedded in the second sealing portion, and another portion of the test strip extends to the second puncture portion.

7. The portable nucleic acid detector according to claim 1, characterized in that: The third puncture member comprises a third sealing portion and a third puncture portion connected to each other, and the third sealing portion is dynamically sealed with the inner wall of the third accommodating channel; An end of the third sealing portion away from the third puncture portion protrudes from the column to provide a force application point.

8. The portable nucleic acid detector according to claim 7, characterized in that: The radial dimension of the third sealing portion is greater than the dimension of the third opening, so that the third sealing portion is confined within the third accommodating channel.

9. The portable nucleic acid detector according to any one of claims 1 to 5 and 7 to 8, characterized in that: The column also includes a plug-in connector, which can seal and connect the column and the sample reaction tube in a plug-in manner.

10. A nucleic acid detection device, characterized in that: include: A nucleic acid extraction tube, which is used to extract nucleic acid to be tested from a biological sample; A sample reaction tube, wherein the sample reaction tube contains a solid amplification reagent for amplifying the nucleic acid to be tested; as well as A portable nucleic acid detector according to any one of claims 1 to 9.

Images