Portable culture bag for stem cell culture

Abstract

The utility model relates to the related technical field of stem cell culture, in particular to a portable culture bag for stem cell culture, which comprises a culture bag, two sides of the culture bag are fixedly connected with connecting strips, one side of each connecting strip is fixedly connected with a first connecting ring, one side of each connecting strip is fixedly connected with a first cavity, and the other side of each connecting strip is fixedly connected with a second cavity. One side of the connecting strip is fixedly connected with a second cavity, one side of the connecting strip is fixedly connected with a third cavity, one side of the connecting strip is fixedly connected with a fourth cavity, and a fixing hole is formed in one side of the connecting strip. According to the stem cell culture bag, the damage of external impact force to cultured stem cells in the culture bag is reduced, meanwhile, under the condition that the liquid inlet, the scavenging port, the waste liquid port and the sampling port are independently formed, different liquid or gas can be input or output through the functional ports, and the situation of mutual pollution in the culture bag is avoided.

Description

Technical Field

[0001] The utility model relates to the technical field related to stem cell culture, in particular to a portable culture bag for stem cell culture. Background Art

[0002] Stem cell culture is a biotechnology that aims to cultivate and expand stem cells for use in research, treatment, and regenerative medicine. The design of the stem cell culture bag aims to simulate the growth environment of stem cells in the body and provide a suitable culture space for stem cells. It can maintain the activity and characteristics of stem cells and promote their proliferation and differentiation. Stem cell culture bags are widely used in stem cell research, tissue engineering, and regenerative medicine. Therefore, there is a special need for a portable stem cell culture bag.

[0003] However, during the use of existing stem cell culture bags, since the stem cell culture bags have no cavity protection, when the culture bags are transferred, the stem cells in the culture bags may be damaged due to environmental factors, thereby reducing research efficiency. At the same time, since the stem cell culture bags have no independent interface and clamping mechanism, contamination may occur when inputting or outputting inside the interface, resulting in a long research sampling time and affecting efficiency. Utility Model Content

[0004] The utility model aims to provide a portable culture bag for stem cell culture, so as to solve the problem that the existing stem cell culture bag proposed in the above background technology has no protective mechanism during use, so when the culture bag is transferred, the stem cells in the culture bag may be damaged due to environmental factors, thereby reducing the research efficiency. At the same time, since the culture bag for stem cell culture has no independent interface and tightening mechanism, contamination may occur when input or output is performed inside the interface, resulting in a long research sampling time and affecting the efficiency.

[0005] To achieve the above-mentioned purpose, the utility model provides the following technical solutions: a portable culture bag for stem cell culture, comprising a culture bag, two sides of the culture bag are fixedly connected with connecting strips, one side of the connecting strip is fixedly connected with a first connecting ring, one end of the culture bag is fixedly connected with a liquid inlet, one end of the culture bag is fixedly connected with a ventilation port, one end of the culture bag is fixedly connected with a waste liquid port, one side of the culture bag is fixedly connected with a sampling port, one end of the connecting strip is provided with a clamping mechanism, one side of the connecting strip is fixedly connected with a first cavity, one side of the connecting strip is fixedly connected with a second cavity, one side of the connecting strip is fixedly connected with a third cavity, one side of the connecting strip is fixedly connected with a fourth cavity, and one side of the connecting strip is provided with a fixing hole;

[0006] The clamping mechanism includes a hose, a fixed block, a sliding groove, a first clamping block, a second clamping block, a screw, a handle, a second connecting ring, a hard tube and a cover, wherein one end of the first connecting ring is fixedly connected to the hose, the surface of the hose is movably sleeved with a fixed block, a sliding groove is provided inside the fixed block, one side of the surface of the sliding groove is fixedly connected to the first clamping block, one side of the surface of the sliding groove is slidably connected to the second clamping block, one side of the surface of the sliding groove is fixedly connected to the screw, the other end of the screw is fixedly connected to the handle, the other end of the hose is fixedly connected to the second connecting ring, the other end of the second connecting ring is fixedly connected to the hard tube, and the surface of the hard tube is threadedly connected to a cover.

[0007] Preferably, the number of the first connecting rings is four groups, and the first connecting rings are fixedly connected to the connecting strips.

[0008] Preferably, the liquid inlet, ventilation port, waste liquid port and sampling port are all fixedly connected to the connecting strip, and the first cavity, the second cavity, the third cavity and the fourth cavity are all fixedly connected to the connecting strip.

[0009] Preferably, the screw rod is threadedly connected to the fixing block, and the second pressing block is slidably connected to the fixing block via the screw rod.

[0010] Preferably, the hard tube is connected to the soft tube via the second connecting ring, and the soft tube is connected to the interior of the culture bag via the first connecting ring.

[0011] Preferably, the material of the hose is polyvinyl chloride (PVC), and the material of the hard tube is polypropylene (PP).

[0012] Preferably, the first cavity, the second cavity, the third cavity and the fourth cavity are all filled with nitrogen.

[0013] Compared with the prior art, the beneficial effects of the utility model are as follows: the portable culture bag for stem cell culture, through the protection setting of the cavity, can avoid external factors such as bumps and other impacts on the cultured stem cells in the culture bag when the culture bag is transferred, thereby improving research efficiency. At the same time, because the culture bag for stem cell culture is provided with an independent interface and a clamping mechanism, contamination is avoided when inputting or outputting inside the interface, reducing the research sampling time, and greatly improving the efficiency of stem cell research. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 This is a schematic diagram of the side view of the appearance structure of the utility model;

[0015] Figure 2 It is a schematic diagram of the exploded structure of part of the clamping mechanism of the utility model;

[0016] Figure 3It is a schematic diagram of the exploded structure of part of the clamping mechanism of the utility model;

[0017] Figure 4 This is a schematic diagram of the top view of the appearance of the cavity of the utility model;

[0018] Figure 5 It is a partial structural schematic diagram of the connecting strip of the utility model.

[0019] In the figure: 1. culture bag; 2. connecting strip; 3. first connecting ring; 4. liquid inlet; 5. ventilation port; 6. waste liquid port; 7. sampling port; 8. clamping mechanism; 801. hose; 802. fixing block; 803. sliding groove; 804. first clamping block; 805. second clamping block; 806. screw; 807. handle; 808. second connecting ring; 809. hard tube; 810. cover; 9. first cavity; 10. second cavity; 11. third cavity; 12. fourth cavity; 13. fixing hole. DETAILED DESCRIPTION

[0020] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0021] See also Figure 1-5 The utility model provides a technical solution: a portable culture bag for stem cell culture, comprising a culture bag 1, connecting strips 2 are fixedly connected to both sides of the culture bag 1, a first connecting ring 3 is fixedly connected to one side of the connecting strip 2, a liquid inlet 4 is fixedly connected to one end of the culture bag 1, a ventilation port 5 is fixedly connected to one end of the culture bag 1, a waste liquid port 6 is fixedly connected to one end of the culture bag 1, a sampling port 7 is fixedly connected to one side of the culture bag 1, a pressing mechanism 8 is provided at one end of the connecting strip 2, a first cavity 9 is fixedly connected to one side of the connecting strip 2, a second cavity 10 is fixedly connected to one side of the connecting strip 2, a third cavity 11 is fixedly connected to one side of the connecting strip 2, a fourth cavity 12 is fixedly connected to one side of the connecting strip 2, and a fixing hole 13 is opened on one side of the connecting strip 2;

[0022] The clamping mechanism 8 includes a hose 801, a fixed block 802, a sliding groove 803, a first clamping block 804, a second clamping block 805, a screw 806, a handle 807, a second connecting ring 808, a hard tube 809 and a cover 810. One end of the first connecting ring 3 is fixedly connected to the hose 801, the surface of the hose 801 is movably sleeved with the fixed block 802, the interior of the fixed block 802 is provided with a sliding groove 803, one side of the surface of the sliding groove 803 is fixedly connected to the first clamping block 804, one side of the surface of the sliding groove 803 is slidably connected to the second clamping block 805, and the second clamping block 809 is fixedly connected to the first clamping block 804. A screw rod 806 is fixedly connected to one side of the block 805, and a handle 807 is fixedly connected to the other end of the screw rod 806. A second connecting ring 808 is fixedly connected to the other end of the hose 801, and a hard tube 809 is fixedly connected to the other end of the second connecting ring 808. A cover 810 is threadedly connected to the surface of the hard tube 809. During use, when the culture bag 1 needs to be transported or moved, the first cavity 9, the second cavity 10, the third cavity 11, and the fourth cavity 12 will play a protective role to a certain extent, buffering the collision load during the transportation or movement of the culture bag 1, and reducing The external impact force can damage the cultured stem cells in the culture bag 1. At the same time, when the liquid inlet 4, the ventilation port 5, the waste liquid port 6, and the sampling port 7 are independently arranged, each functional interface can input or output different liquids or gases to avoid mutual contamination in the culture bag 1. During use, by manually rotating the handle 807, the rotation of the handle 807 will drive the screw 806 to rotate, and the rotation of the screw 806 will drive the second pressing block 805 to move. When the second pressing block 805 moves close to the first pressing block 804, the first pressing block 804 The interaction with the second clamping block 805 forces the hose 801 to close. When the second clamping block 805 moves to the farthest distance from the first clamping block 804, the hose 801 is in an unobstructed state. When liquid or gas needs to be input or output to a certain functional interface, the cover 810 can be manually unscrewed, and then the handle 807 can be manually unscrewed to ensure that the entire functional interface remains unobstructed. When a certain functional interface completes the input or output of liquid or gas, the handle 807 is first tightened to close the hose 801, and then the cover 810 is tightened to achieve secondary closure of the certain functional interface.

[0023] Furthermore, the number of first connecting rings 3 is four groups, and the first connecting rings 3 are fixedly connected to the connecting strips 2. By setting four groups of first connecting rings 3, the components of each functional interface can be unified to reduce costs. By setting the first connecting rings 3 and the connecting strips 2, the culture bag 1 and each functional interface can be integrated as a whole.

[0024] Furthermore, the liquid inlet 4, the ventilation port 5, the waste liquid port 6, and the sampling port 7 are all fixedly connected to the connecting strip 2, and the first cavity 9, the second cavity 10, the third cavity 11, and the fourth cavity 12 are all fixedly connected to the connecting strip 2. Through the arrangement of the liquid inlet 4, the ventilation port 5, the waste liquid port 6, the sampling port 7 and the connecting strip 2, the connection between the liquid inlet 4, the ventilation port 5, the waste liquid port 6, the sampling port 7 and the clamping mechanism 8 can be made more stable and durable. Through the arrangement of the first cavity 9, the second cavity 10, the third cavity 11, the fourth cavity 12 and the connecting strip 2, the connection between the first cavity 9, the second cavity 10, the third cavity 11, the fourth cavity 12 and the various components of the connecting strip 2 can be made more stable.

[0025] Furthermore, the screw 806 is threadedly connected to the fixed block 802, and the second clamping block 805 is slidingly connected to the fixed block 802 through the screw 806. Through the setting of the screw 806 and the fixed block 802, the screw 806 and the fixed block 802 will convert the rotational motion into linear motion. Through the setting of the second clamping block 805, the screw 806 and the fixed block 802, the second clamping block 805 can cooperate with the first clamping block 804 to clamp the hose 801, so as to achieve the effect of closing the functional interface.

[0026] Furthermore, the hard tube 809 is connected to the hose 801 through the second connecting ring 808, and the hose 801 is connected to the inside of the culture bag 1 through the first connecting ring 3. Through the arrangement of the hard tube 809, the second connecting ring 808 and the hose 801, the cover 810 can seal the hard tube 809, and at the same time, the hose 801 is also sealed for a second time, thereby protecting the growth conditions of the cultured stem cells in the culture bag 1.

[0027] Furthermore, the material of the hose 801 is polyvinyl chloride PVC, and the material of the hard tube 809 is polypropylene PP. By setting the hose 801 made of polyvinyl chloride PVC, polyvinyl chloride PVC has good strength and durability, and polyvinyl chloride PVC has good chemical stability. By setting the hard tube 809 made of polypropylene PP, polypropylene PP helps to connect the hose 801 and ensure the sealing and stability of the hose 801.

[0028] Furthermore, the first cavity 9, the second cavity 10, the third cavity 11, and the fourth cavity 12 are all filled with nitrogen. Through the setting of the first cavity 9, the second cavity 10, the third cavity 11, and the fourth cavity 12, nitrogen is an inert gas that is tasteless, odorless, non-toxic, harmless to the human body, and non-flammable. Nitrogen has good stability and inertness, can provide suitable support and pressure distribution, and avoids the safety risks that may be caused by gases such as oxygen.

[0029] Working principle: During use, when it is necessary to input or output liquid or gas to a certain functional interface, the cover 810 can be manually unscrewed, and then the handle 807 can be manually unscrewed to ensure that the entire functional interface remains unobstructed. When a certain functional interface completes the input or output of liquid or gas, the handle 807 is first tightened to close the hose 801, and then the cover 810 is tightened to achieve secondary closure of the functional interface. When the culture bag 1 needs to be transported or moved, the first cavity 9, the second cavity 10, the third cavity 11, and the fourth cavity 12 will play a protective role to a certain extent, buffering the collision load during the transportation or movement of the culture bag 1, and reducing the damage of the external impact force to the cultured stem cells in the culture bag 1. At the same time, when the liquid inlet 4, the ventilation port 5, the waste liquid port 6, and the sampling port 7 are independently set, each functional interface can input or output different liquids or gases to avoid mutual contamination in the culture bag 1.

[0030] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A portable culture bag for stem cell culture, comprising a culture bag (1), characterized in that: The culture bag (1) is fixedly connected to connecting strips (2) on both sides, and a first connecting ring (3) is fixedly connected to one side of the connecting strip (2). One end of the culture bag (1) is fixedly connected to a liquid inlet (4), one end of the culture bag (1) is fixedly connected to a ventilation port (5), one end of the culture bag (1) is fixedly connected to a waste liquid port (6), one side of the culture bag (1) is fixedly connected to a sampling port (7), one end of the connecting strip (2) is provided with a clamping mechanism (8), one side of the connecting strip (2) is fixedly connected to a first cavity (9), one side of the connecting strip (2) is fixedly connected to a second cavity (10), one side of the connecting strip (2) is fixedly connected to a third cavity (11), one side of the connecting strip (2) is fixedly connected to a fourth cavity (12), and one side of the connecting strip (2) is provided with a fixing hole (13); The clamping mechanism (8) comprises a hose (801), a fixed block (802), a sliding groove (803), a first clamping block (804), a second clamping block (805), a screw (806), a handle (807), a second connecting ring (808), a hard tube (809) and a cover (810), wherein one end of the first connecting ring (3) is fixedly connected to the hose (801), the surface of the hose (801) is movably sleeved with the fixed block (802), the interior of the fixed block (802) is provided with a sliding groove (803), and the sliding groove (803) is ) is fixedly connected to one side of the surface of the first clamping block (804), and a second clamping block (805) is slidably connected to one side of the surface of the sliding groove (803), and a screw rod (806) is fixedly connected to one side of the second clamping block (805), and the other end of the screw rod (806) is fixedly connected to a handle (807), and the other end of the hose (801) is fixedly connected to a second connecting ring (808), and the other end of the second connecting ring (808) is fixedly connected to a hard tube (809), and a sealing cover (810) is threadedly connected to the surface of the hard tube (809).

2. A portable culture bag for stem cell culture according to claim 1, characterized in that: The number of the first connecting rings (3) is four groups, and the first connecting rings (3) are fixedly connected to the connecting strip (2).

3. A portable culture bag for stem cell culture according to claim 1, characterized in that: The liquid inlet (4), the ventilation port (5), the waste liquid port (6), and the sampling port (7) are all fixedly connected to the connecting strip (2), and the first cavity (9), the second cavity (10), the third cavity (11), and the fourth cavity (12) are all fixedly connected to the connecting strip (2).

4. A portable culture bag for stem cell culture according to claim 1, characterized in that: The screw rod (806) is threadedly connected to the fixed block (802), and the second pressing block (805) is slidably connected to the fixed block (802) via the screw rod (806).

5. A portable culture bag for stem cell culture according to claim 1, characterized in that: The hard tube (809) is connected to the hose (801) via the second connecting ring (808), and the hose (801) is connected to the interior of the culture bag (1) via the first connecting ring (3).

6. A portable culture bag for stem cell culture according to claim 1, characterized in that: The material of the hose (801) is polyvinyl chloride (PVC), and the material of the hard tube (809) is polypropylene (PP).

7. A portable culture bag for stem cell culture according to claim 1, characterized in that: The first cavity (9), the second cavity (10), the third cavity (11), and the fourth cavity (12) are all filled with nitrogen.

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