Magnetic bead separation device

Abstract

The utility model relates to a magnetic bead separation device which comprises an upper fixed supporting plate, a lower fixed supporting plate, a magnetic field area vertically arranged between the upper fixed supporting plate and the lower fixed supporting plate, and a container arranged in the magnetic field area and used for bearing a magnetic bead sample to be separated, the magnetic field area comprises a plurality of first magnetic plates vertically arranged between the upper fixed supporting plate and the lower fixed supporting plate, and second magnetic plates located between the first magnetic plates and slidably connected with the upper fixed supporting plate and the lower fixed supporting plate. Compared with the prior art, the magnetic bead separation device can improve the treatment capacity to the greatest extent while providing a uniform magnetic field, and can quickly, efficiently and uniformly adsorb magnetic particles under the action of the magnetic field, so that the magnetic particles are separated from a solution, and the adsorption efficiency is high; the magnetic field intensity of the central point in the magnetic field space is controlled by adjusting different distances between the magnetic plates, so that particles with different magnetic intensities are treated in a targeted manner, and the treatment efficiency is improved.

Description

Technical Field

[0001] The utility model relates to the technical field of biomedical equipment, in particular to a magnetic bead separation device. Background Art

[0002] Magnetic beads refer to a type of biomedical material with separation or adsorption functions. Due to their small particle size, large specific surface area, strong adsorption capacity, easy separation and purification, and easy automation, they are widely used in nucleic acid extraction, protein purification, cell sorting, chemiluminescence and other fields. Due to their magnetic properties, magnetic beads dispersed in a solution can be quickly adsorbed and enriched under the action of an external magnetic field to achieve separation, purification and other operations.

[0003] However, in existing magnetic bead separation devices, due to the small area of ​​the external magnet and the short range of the magnetic field, most separation devices have low processing capacity for magnetic bead solutions, and the manpower and time costs in the washing process are high, which is not conducive to industrial production. For example, patent CN206684167U discloses a magnetic bead separation device with controllable magnetism, and the maximum processing capacity of the device is only 200mL.

[0004] Therefore, the use of more efficient and uniform magnetic bead separation devices is of great significance in shortening separation time and increasing processing volume. Summary of the invention

[0005] The purpose of the utility model is to provide a magnetic bead separation device in order to overcome the defects in the prior art.

[0006] The purpose of the utility model can be achieved through the following technical solutions:

[0007] The technical solution of the utility model is to provide a magnetic bead separation device, including an upper fixed support plate, a lower fixed support plate, a magnetic field area erected between the upper fixed support plate and the lower fixed support plate, and a container arranged in the magnetic field area and carrying a magnetic bead sample to be separated, wherein the magnetic field area includes a plurality of first magnetic plates erected between the upper fixed support plate and the lower fixed support plate, and a second magnetic plate located between the first magnetic plates and slidably connected to the upper fixed support plate and the lower fixed support plate.

[0008] In some specific embodiments, a plurality of magnets are disposed on the side walls of the first magnetic plate and the second magnetic plate.

[0009] In some specific embodiments, the directions of the magnet poles on the side walls of the first magnetic plate and the second magnetic plate are respectively consistent, and the directions of the magnet poles on the opposite side walls of two adjacent magnetic plates are opposite.

[0010] In some embodiments, the magnet is a permanent magnet.

[0011] Preferably, the magnet is a sintered NdFeB permanent magnet.

[0012] In some specific embodiments, the effective area of ​​the first magnetic plate is greater than the effective area of ​​the second magnetic plate.

[0013] Preferably, the effective area of ​​the first magnetic plate is twice the effective area of ​​the second magnetic plate.

[0014] In some specific embodiments, a plurality of first slide grooves parallel to the first magnetic plate are provided on the bottom of the upper fixed support plate, a second slide groove corresponding to the first slide groove is provided on the top of the lower fixed support plate, and sliders slidably connected to the first slide groove and the second slide groove are respectively provided on the top and bottom of the second magnetic plate.

[0015] In some embodiments, the container is a transparent container without a lid.

[0016] In some specific embodiments, a water inlet is provided at an upper end of one side wall of the container, and a water outlet is provided at a lower end of an opposite side wall.

[0017] In some specific embodiments, the water inlet is located at the upper end of the central axis of one side wall of the container, and the water outlet is located at the lower end of the central axis of another side wall of the container.

[0018] Compared with the prior art, the utility model has the following beneficial effects:

[0019] The utility model arranges magnets on magnetic plates standing opposite to each other to form a uniform magnetic field space in the magnetic plate space, increases the magnetic field working distance through the interaction between magnets with relative magnetic poles, and enables the magnetic bead separation device to maximize the processing capacity while providing a uniform magnetic field. Under the action of the magnetic field, the magnetic particles are quickly, efficiently and uniformly adsorbed, the magnetic particles are distinguished from the solution, and the adsorption efficiency is high. In addition, the utility model controls the magnetic field strength of the center point in the magnetic field space by adjusting the different spacings between the magnetic plates, so as to specifically process particles with different magnetic strengths and improve the processing efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 It is a structural schematic diagram of the magnetic field operation part of the utility model.

[0021] Figure 2 It is a structural schematic diagram of the container of the utility model.

[0022] Figure 3 It is a schematic diagram of a use example of the utility model.

[0023] Figure 4 This is another schematic diagram of an example of use of the present invention.

[0024] The following are marked in the figure:

[0025] 1 is an upper fixed support plate, 2 is a first magnetic plate, 3 is a second magnetic plate, 4 is a magnet, 5 is a container, 6 is a water inlet, 7 is a water outlet, and 8 is a lower fixed support plate. DETAILED DESCRIPTION

[0026] The present invention is described in detail below in conjunction with the accompanying drawings and specific embodiments. This embodiment is implemented based on the technical solution of the present invention, and provides a detailed implementation method and a specific operation process, but the protection scope of the present invention is not limited to the following embodiments.

[0027] It should be noted that similar reference numerals and letters denote similar items in the following drawings, and therefore, once an item is defined in one drawing, further definition and explanation thereof is not required in subsequent drawings.

[0028] In conjunction with the accompanying drawings, some embodiments of the present invention are described in detail below. In the absence of conflict, the following embodiments and features in the embodiments can be combined with each other.

[0029] In the following embodiments, unless otherwise specified, functional components or structures are conventional components or conventional structures used in the art to achieve corresponding functions.

[0030] Embodiment 1:

[0031] The present embodiment provides a magnetic bead separation device, including an upper fixed support plate 1, a lower fixed support plate 8, a magnetic field area erected between the upper fixed support plate 1 and the lower fixed support plate 8, and a container 5 arranged in the magnetic field area and carrying a magnetic bead sample to be separated, wherein the magnetic field area includes a plurality of first magnetic plates 2 erected between the upper fixed support plate 1 and the lower fixed support plate 8, and a second magnetic plate 3 located between the first magnetic plates 2 and slidably connected to the upper fixed support plate 1 and the lower fixed support plate 8. Both the upper fixed support plate 1 and the lower fixed support plate 8 have a certain stability to prevent the magnetic plates from attracting each other. A plurality of magnets 4 are arranged on the side walls of the first magnetic plate 2 and the second magnetic plate 3. Preferably, the magnet 4 is a sintered NdFeB permanent magnet to achieve the largest possible increase in the magnetic field area under the premise of saving costs. The magnetic pole directions of the magnets 4 on the side walls of the first magnetic plate 2 and the second magnetic plate 3 are respectively consistent, and the magnetic pole directions of the magnets on the opposite side walls of the two adjacent magnetic plates are opposite, which is used to strengthen the magnetic field strength and action distance of the magnetic field area. The effective area of ​​the first magnetic plate 2 is greater than the effective area of ​​the second magnetic plate 3. For example, the effective area of ​​the first magnetic plate 2 is twice the effective area of ​​the second magnetic plate 3. Figure 1As shown, the effective area of ​​the first magnetic plate 2 is 2d*h, and the effective area of ​​the second magnetic plate 3 is d*h. A plurality of first slide grooves parallel to the first magnetic plate 2 are provided on the bottom of the upper fixed support plate 1, and a second slide groove corresponding to the first slide groove is provided on the top of the lower fixed support plate 8. Slide blocks slidably connected to the first slide groove and the second slide groove are provided on the top and bottom of the second magnetic plate 3, respectively. Since the distances from the second magnetic plate 3 to the two first magnetic plates 2 are different, and the distances from the ends of the first magnetic plates 2 are also different, the magnetic field area of ​​the utility model can be divided into three areas of different strengths, namely, the space between the second magnetic plate 3 and the two first magnetic plates 2, and the space between the two first magnetic plates 2. For example, as Figure 1 As shown, one end of the first magnetic plate 2 and the second magnetic plate 3 are flush, the distance between the second magnetic plate 3 and one first magnetic plate 2 is b, the distance between the second magnetic plate 3 and another first magnetic plate 2 is a, and the distance between the two first magnetic plates 2 is c. Therefore, the sizes of the three regions of different strengths divided by the magnetic field are a*d*h, b*d*h, and c*d*h, respectively. This technical solution adjusts the mutual attraction between the magnets 4 by adjusting the relative distance between the magnetic plates. The variation of the attraction of the magnets 4 with the distance can be expressed by the following formula: F(d) = K*(m1*m2) / d 2 , where F(d) is the magnetic attraction, K is a constant, m1 is the magnetic moment of magnet 1, m2 is the magnetic moment of magnet 2, and d is the distance between the two magnets. It can be seen that the attraction between the magnets is inversely proportional to the distance. For magnetic bead solutions with stronger magnetism, they can be separated in a large-pitch magnetic field space to improve processing capacity; for magnetic bead solutions with weaker magnetism, they can be separated in a small-pitch magnetic field space to speed up adsorption time.

[0032] like Figure 2-4 As shown, the container 5 is a transparent uncovered container. Preferably, the container 5 is also a plastic container, which is convenient for observing the adsorption of magnetic beads inside the container 5. In addition, the container 5 is an uncovered open structure, which is convenient for material input and container cleaning. A water inlet 6 is provided at the upper end of one side wall of the container 5, and a water outlet 7 is provided at the lower end of the opposite side wall. Exemplarily, the water inlet 6 is located at the upper end of the central axis of one side wall of the container 5 to ensure that the inflowing magnetic beads are evenly adsorbed on the magnetic plates on both sides, and the water outlet 7 is located at the lower end of the central axis of the other side wall of the container 5. After the magnetic beads are completely adsorbed on the side wall, the clarified waste liquid can be discharged through the water outlet 7 to ensure that the adsorbed magnetic beads are not disturbed when the waste liquid is discharged and can be completely discharged. In addition, the water inlet 6 and the water outlet 7 can be matched with pipelines and pumps to achieve automated processing.

[0033] Exemplarily, the container 5 is of size c*d*h and is placed between the two first magnetic plates 2. When the water inlet 6 and the water outlet 7 of the container 5 are closed, the magnetic bead solution can be poured in through the top opening; the water inlet 6 can also be opened, and the connecting pipe and the pump flow into the magnetic bead solution from the water inlet 6, which is convenient for automation. The container 5 containing the magnetic bead solution keeps the water inlet 6 and the water outlet 7 closed, and is placed between the two first magnetic plates 2 until the magnetic beads are completely adsorbed to the side, and the solution is in a clear state. The water outlet 7 can be opened to allow the waste liquid to flow out and retain the magnetic beads. If the magnetic bead solution cannot be completely separated under the magnetic field strength or the separation time is long, a smaller container can be replaced, such as a container 5 of size a*d*h, which is placed in the magnetic field gap between the corresponding first magnetic plate 2 and the second magnetic plate 3, so that the magnetic beads can be separated by magnetic adsorption faster, although a certain processing capacity is sacrificed, but the processing time is saved.

[0034] In summary, the magnetic bead separation device provided by the utility model is suitable for fast, efficient, continuous and automated separation and washing operations of batch magnetic beads in industrial production. It not only improves the spatial size and intensity of the magnetic field and improves the processing capacity while controlling costs, but also can optimize the appropriate magnetic field strength according to the properties of the magnetic beads to be separated. It has a wide range of applications and meets the efficiency and quality of magnetic bead separation and washing.

[0035] The above description of the embodiments is to facilitate the understanding and use of the utility model by those skilled in the art. It is obvious that those familiar with the art can easily make various modifications to these embodiments and apply the general principles described herein to other embodiments without creative work. Therefore, the utility model is not limited to the above embodiments, and improvements and modifications made by those skilled in the art based on the disclosure of the utility model without departing from the scope of the utility model should be within the scope of protection of the utility model.

Claims

1. A magnetic bead separation device, characterized in that: The invention comprises an upper fixed support plate (1), a lower fixed support plate (8), a magnetic field area vertically arranged between the upper fixed support plate (1) and the lower fixed support plate (8), and a container (5) arranged in the magnetic field area and carrying a magnetic bead sample to be separated. The magnetic field area comprises a plurality of first magnetic plates (2) vertically arranged between the upper fixed support plate (1) and the lower fixed support plate (8), and a second magnetic plate (3) located between the first magnetic plates (2) and slidably connected to the upper fixed support plate (1) and the lower fixed support plate (8).

2. The magnetic bead separation device according to claim 1, characterized in that: A plurality of magnets (4) are provided on the side walls of the first magnetic plate (2) and the second magnetic plate (3).

3. The magnetic bead separation device according to claim 2, characterized in that: The magnetic pole directions of the magnets (4) on the side walls of the first magnetic plate (2) and the second magnetic plate (3) are respectively consistent, and the magnetic pole directions of the magnets on the opposite side walls of two adjacent magnetic plates are opposite.

4. The magnetic bead separation device according to claim 2, characterized in that: The magnet (4) is a permanent magnet.

5. The magnetic bead separation device according to claim 1, characterized in that: The effective area of ​​the first magnetic plate (2) is greater than the effective area of ​​the second magnetic plate (3).

6. The magnetic bead separation device according to claim 5, characterized in that: The effective area of ​​the first magnetic plate (2) is twice the effective area of ​​the second magnetic plate (3).

7. The magnetic bead separation device according to claim 1, characterized in that: The bottom of the upper fixed support plate (1) is provided with a plurality of first slide grooves parallel to the first magnetic plate (2), the top of the lower fixed support plate (8) is provided with second slide grooves corresponding to the first slide grooves, and the top and bottom of the second magnetic plate (3) are respectively provided with sliders slidably connected to the first slide grooves and the second slide grooves.

8. The magnetic bead separation device according to claim 1, characterized in that: The container (5) is a transparent container without a cover.

9. The magnetic bead separation device according to claim 8, characterized in that: A water inlet (6) is provided at the upper end of one side wall of the container (5), and a water outlet (7) is provided at the lower end of the opposite side wall.

10. The magnetic bead separation device according to claim 9, characterized in that: The water inlet (6) is located at the upper end of the central axis of one side wall of the container (5), and the water outlet (7) is located at the lower end of the central axis of the other side wall of the container (5).

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