NK cell amplification kit
By designing a stable clamping assembly and UV disinfection system, the damage and contamination of the reagent bottles of the NK cell amplification kit during transportation and use are solved, and the stable storage and sterile environment of the test tube are achieved.
Patent Information
- Application Number
- CN202421138458.9
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-05-23
- Publication Date
- 2025-01-03
- Estimated Expiration
- 2034-05-23
AI Technical Summary
The existing NK cell amplification kits are prone to damage and cell contamination during delivery and use, and lack effective protective measures.
An NK cell amplification kit including an open-up box, a hinged cover plate, a transverse fixed placement plate, a plurality of storage holes and a clamping assembly is designed, and the test tube is stably clamped using the clamping assembly and the limiting groove. The cover plate and light shield are combined with ultraviolet lamps to disinfect bacteria to ensure a sterile environment.
Effectively prevents test tubes from shaking and damage, ensures the sterile environment of NK cells, and improves the practicality and safety of the kit.
Smart Images
Figure CN222292371U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of cell culture, in particular to a NK cell amplification kit. Background Art
[0002] NK cells, or Natural Killer cells, are an important type of cell in the human immune system. They are a type of lymphocytes and are mainly found in the blood, lymphatic tissues, and certain organs. The main function of NK cells is to recognize and kill virus-infected cells and certain cancer cells. Unlike T cells, which need to recognize specific antigens in advance, NK cells can directly recognize and attack abnormal cells, so they are called "natural" killers.
[0003] An amplification kit is usually used for NK cell culture. Most of the existing amplification kits are lacking in protection. Since they are placed in the amplification kit, they will inevitably shake during transportation, causing the reagent bottles to easily collide and possibly be damaged. At the same time, when the staff takes the reagent bottles, the bacteria on their hands are easily brought into the amplification kit, causing the NK cells to be contaminated. Utility Model Content
[0004] In view of the technical problems in the above-mentioned prior art, the utility model provides a NK cell expansion kit, the purpose of which is to solve the above-mentioned problems.
[0005] In order to achieve the above purpose, the utility model provides the following technical solutions:
[0006] A NK cell expansion kit comprises a box body with an opening upward, wherein a test tube is placed in the box body, a cover plate is hingedly connected to the upper side wall of the box body, a placement plate is fixedly arranged horizontally in the box body, a plurality of storage holes are evenly arranged on the placement plate corresponding to the test tubes, a plurality of clamping components are movably arranged on the inner walls of the storage holes corresponding to the test tubes, ultraviolet lamps are arranged around the top of the inner side wall of the box body, a shading plate is arranged above the placement plate, and through holes are arranged on the surface of the shading plate corresponding to the test tubes.
[0007] Preferably, the clamping assembly includes four longitudinally arranged clamping blocks, the four clamping blocks are arranged in a circular array and the inner wall of the storage hole, the inner wall of the storage hole is respectively provided with active cavities corresponding to the four clamping blocks, the clamping block extends into the active cavity toward one side of the active cavity and is slidably connected thereto, a limit block is provided at one end of the clamping block located in the active cavity, a spring is provided at one end of the limit block facing away from the clamping block, one end of the spring is fixed on the limit block, and the other end is fixed on the side wall of the active cavity opposite to the limit block.
[0008] Preferably, the upper sides of the four clamping blocks are each provided with an inclined surface slanting downward toward the middle of the storage hole.
[0009] Preferably, arc-shaped grooves are provided at the clamping parts of the four clamping blocks corresponding to the test tubes.
[0010] Preferably, a limiting groove is provided at the bottom of the box body corresponding to the bottom of the test tube.
[0011] Preferably, a light shield is fixedly provided on the lower side of the cover plate corresponding to the test tube, and when the cover plate is closed, the lower side of the light shield is just in contact with the light shield.
[0012] Compared with the prior art, the utility model has the following beneficial effects:
[0013] The utility model provides a NK cell expansion kit. When storing, the cover plate is opened, and the test tube is inserted into the storage hole. When the bottom of the test tube abuts against the inclined surface of the clamping block, the spring can be compressed downward to retract the clamping block into the movable cavity, so that the test tube can be smoothly moved down to the bottom to abut against the limiting groove. The clamping block clamps the test tube with the spring force, so that the test tube is stabilized in the storage hole, so that the test tube will not shake during transportation, and the test tube will not be damaged.
[0014] After taking out the test tube or placing it to sterilize the bacteria at its mouth, close the cover, use the hood to shield the test tube, and then use ultraviolet light to sterilize the bacteria entering from the outside into the box, ensuring a sterile environment inside the box to prevent NK cells from being contaminated. At the same time, the combination of the hood and the ultraviolet light will not affect the NK cells when the ultraviolet light is used to sterilize the bacteria, and is highly practical. BRIEF DESCRIPTION OF THE DRAWINGS
[0015] Figure 1 This is a front internal schematic diagram of a NK cell expansion kit described in the utility model;
[0016] Figure 2 This is an enlarged view of point A of a NK cell expansion kit described in the utility model;
[0017] Figure 3 It is a top view schematic diagram of a NK cell expansion kit described in the utility model;
[0018] Figure 4 This is an enlarged view of point B of the NK cell expansion kit described in the present invention.
[0019] In the figure: 1. box body; 11. cover plate; 12. limiting groove; 13. light shield; 2. test tube; 3. placement plate; 31. storage hole; 32. movable cavity; 4. clamping assembly; 41. clamping block; 42. limiting block; 43. spring; 44. inclined surface; 45. arc groove; 5. ultraviolet lamp; 6. light shield; 61. through hole. DETAILED DESCRIPTION
[0020] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.
[0021] See also Figures 1 to 4 In the embodiment proposed in the present application, there is: an NK cell expansion kit, comprising a box body 1 with an opening upward, a test tube 2 is placed in the box body 1, a cover plate 11 is hingedly connected to the upper side wall of the box body 1, a placement plate 3 is fixedly arranged in the box body 1, a plurality of storage holes 31 are evenly arranged on the placement plate 3 corresponding to the test tube 2, a plurality of storage holes 31 are movably arranged on the inner walls corresponding to the test tubes 4, a limited groove 12 is arranged at the bottom of the box body 1 corresponding to the bottom of the test tube 2, ultraviolet lamps 5 are arranged around the top of the inner side wall of the box body 1, a shading plate 6 is arranged above the placement plate 3, a through hole 61 is arranged on the surface of the shading plate 6 corresponding to the test tube 2, a shading cover 13 is fixedly arranged on the lower side of the cover plate 11 corresponding to the test tube 2, and when the cover plate 11 is closed, the lower side of the shading cover 13 just abuts against the shading plate 6;
[0022] When storing, the cover 11 is opened and the test tube 2 is inserted into the storage hole 31. The test tube 2 is stabilized in the storage hole 31 by the clamping of the clamping assembly 4 and the limiting groove 12, so that it will not shake during transportation and the test tube will not be damaged. At the same time, after the test tube 2 is taken or placed to sterilize its tube mouth, the cover 11 is covered, the shading hood 13 shields the test tube 2, and then the bacteria entering from the outside into the box body 1 are sterilized by the ultraviolet lamp 5, so as to ensure a sterile environment in the box body 1 and avoid contamination of NK cells. At the same time, the cooperation of the shading plate 6 and the shading hood 13 will not affect the NK cells when the ultraviolet lamp 5 kills bacteria, and the practicability is high.
[0023] In another embodiment, see Figures 1 to 4The clamping assembly 4 includes four clamping blocks 41 arranged longitudinally, and the four clamping blocks 41 are arranged in a circular array and on the inner side wall of the storage hole 31. The upper sides of the four clamping blocks 41 are provided with an inclined surface 44 inclined downward toward the middle of the storage hole 31, and the clamping portion thereof is provided with an arc groove 45 at the position corresponding to the test tube 2. The inner side wall of the storage hole 31 is provided with movable cavities 32 corresponding to the four clamping blocks 41, respectively. The clamping block 41 extends into the movable cavity 32 toward one side of the movable cavity 32 and is slidably connected thereto. A limiting block 42 is provided at one end of the clamping block 41 located in the movable cavity 32, and a spring 43 is provided at one end of the limiting block 42 facing away from the clamping block 41, and one end of the spring 43 is fixed on the limiting block 42, and the other end is fixed on the side wall of the movable cavity 32 opposite to the limiting block 42;
[0024] When storing the test tube 2, the cover 11 is opened and the test tube 2 is inserted into the storage hole 31. When the bottom of the test tube 2 abuts against the inclined surface 44 of the clamping block 41, the spring 43 can be compressed downward to retract the clamping block 41 into the movable cavity 32, so that the test tube 2 can be smoothly moved down to the bottom to abut against the limiting groove 12. The clamping block 41 clamps the test tube 2 with the elastic force of the spring 43, thereby stabilizing the test tube 2 in the storage hole 31. The setting of the arc groove 45 makes it fit the wall of the test tube 2 more closely when clamping the test tube 2, making the clamping more stable, so that there will be no shaking during transportation, and the test tube 2 will not be damaged.
[0025] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device. In the absence of further restrictions, the elements defined by the sentence "comprise a ..." do not exclude the existence of other identical elements in the process, method, article or device including the elements.
[0026] In the description of the present invention, unless otherwise specified, "plurality" means two or more; the terms "upper", "lower", "left", "right", "inside", "outside", "front end", "rear end", "head", "tail", etc. indicate directions or positional relationships based on the directions or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the referred device or element must have a specific direction, be constructed and operated in a specific direction, and therefore cannot be understood as a limitation on the present invention.
[0027] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.
Claims
1. A NK cell expansion kit, comprising a box body (1) with an opening facing upward, a test tube (2) placed in the box body (1), and a cover plate (11) hingedly connected to the upper side wall of the box body (1), characterized in that: A placement plate (3) is fixedly disposed transversely in the box body (1), a plurality of storage holes (31) are evenly disposed on the placement plate (3) corresponding to the test tubes (2), a plurality of clamping components (4) are movably disposed on the inner walls of the storage holes (31) corresponding to the test tubes, ultraviolet lamps (5) are disposed around the top of the inner side wall of the box body (1), a shading plate (6) is disposed above the placement plate (3), and a through hole (61) is disposed on the surface of the shading plate (6) corresponding to the test tubes (2).
2. A NK cell expansion kit according to claim 1, characterized in that: The clamping assembly (4) comprises four clamping blocks (41) arranged longitudinally, the four clamping blocks (41) are arranged in a circular array and on the inner wall of the storage hole (31), the inner wall of the storage hole (31) is respectively provided with movable cavities (32) corresponding to the four clamping blocks (41), the clamping block (41) extends into the movable cavity (32) towards one side of the movable cavity (32) and is slidably connected thereto, a limiting block (42) is provided at one end of the clamping block (41) located in the movable cavity (32), a spring (43) is provided at one end of the limiting block (42) facing away from the clamping block (41), one end of the spring (43) is fixed on the limiting block (42), and the other end is fixed on the side wall of the movable cavity (32) opposite to the limiting block (42).
3. A NK cell expansion kit according to claim 2, characterized in that: The upper sides of the four clamping blocks (41) are each provided with an inclined surface (44) which is inclined downward toward the middle of the storage hole (31).
4. A NK cell expansion kit according to claim 2, characterized in that: The clamping parts of the four clamping blocks (41) are provided with arc grooves (45) at locations corresponding to the test tube (2).
5. The NK cell expansion kit according to claim 1, characterized in that: The bottom of the box body (1) is provided with a limiting groove (12) corresponding to the bottom of the test tube (2).
6. The NK cell expansion kit according to claim 1, characterized in that: A light shield (13) is fixedly provided on the lower side of the cover plate (11) corresponding to the test tube (2); when the cover plate (11) is closed, the lower side of the light shield (13) is in contact with the light shield plate (6).