Gravity liquid supply overturning blowing device for chip synthesizer

Abstract

The utility model discloses a gravity liquid supply turning blowing device for a chip synthesizer, which comprises a cabin body, and a chip placing seat, a turning cover mechanism, a rotating shaft, a turning driving unit, a gas and liquid supply supporting mechanism and a locking mechanism which are arranged in the cabin body, two ends of the rotating shaft are rotatably connected with two ends of the cabin body, and the turning cover mechanism is driven by the turning driving unit to rotate; the chip placing seat is fixed relative to the cabin body through a horizontal positioning mechanism, and the horizontal positioning mechanism is mounted on the outer wall of the cabin body; the cover turning mechanism comprises a cover frame, an upper cover and a plurality of pressing units, and acting force can be applied to the upper cover in the direction perpendicular to the plane where the cover frame is located through the pressing units. The locking mechanism is clamped into the groove to clamp the cover turning mechanism on the cabin body, and the cover turning mechanism is located above the chip placing seat. The chip synthesis device is high in function integration, and multiple functions such as chip cleaning, public reagent supply, reagent replacement, reagent cleaning, chip blowing and chip drying required in the chip synthesis process are achieved.

Description

Technical Field

[0001] The utility model belongs to the technical field of gene synthesis supporting equipment, and particularly relates to a gravity liquid supply flipping and sweeping device for a chip synthesizer. Background Art

[0002] DNA chip technology is a new technology that emerged in the 1990s and can detect hundreds, thousands, or even tens of thousands of genes at the same time. It has the characteristics of high throughput and parallelism, and has shown broad application prospects in many fields such as gene expression spectrum analysis, detection of gene mutations and polymorphism analysis, drug screening, and sequence analysis. With the continuous deepening of research and the continuous development of technology, biochips will surely play an extraordinary role in the field of life science research. Therefore, it is inevitable to involve chip synthesis equipment, commonly known as chip synthesizer.

[0003] The chip synthesis process requires multiple steps and processes, including chip cleaning, supplying common reagents, replacing reagents, cleaning reagents, purging chips, drying chips, etc. The integration of the above functions in existing synthesizers is low, and the degree of automation is also low, that is, the ability to quickly post-process the chip during chip synthesis is limited. In addition, the structural design of each part of the existing chip synthesizer is not compact enough, the layout is not reasonable enough, and there are problems such as large size.

[0004] The utility model aims to provide a gravity liquid supply flipping and sweeping device for a chip synthesizer, which can solve the above-mentioned problem. Utility Model Content

[0005] The main technical problem solved by the utility model is to provide a gravity liquid supply flip purge device for a chip synthesizer, which has strong functional integration and realizes multiple functions such as chip cleaning, supply of common reagents, reagent replacement, reagent cleaning, chip purge, chip drying, etc. required in the chip synthesis process; this device solves the need for rapid post-processing of chips during the chip synthesis process.

[0006] In order to solve the above technical problems, a technical solution adopted by the utility model is as follows: the utility model provides a gravity-feed liquid flip-purge device for a chip synthesizer, comprising a cabin and a chip placement seat arranged on the cabin, a flip mechanism, a rotating shaft, a flip drive unit, a gas-liquid support mechanism and a locking mechanism, wherein the cabin has a accommodating space inside, the two ends of the rotating shaft are rotatably connected to the two ends of the cabin, one side of the flip mechanism is sleeved on the rotating shaft, and the flip mechanism can be driven to rotate by the flip drive unit; one side of the chip placement seat is sleeved on the rotating shaft, and the chip placement seat is fixed relative to the cabin by a horizontal positioning mechanism, and the horizontal positioning mechanism is installed at the outer wall of the cabin;

[0007] The gas supply liquid support mechanism is installed outside the cabin and can communicate with the interior of the cabin;

[0008] The flip cover mechanism comprises a cover frame, an upper cover and a plurality of pressing units, wherein the pressing units are installed on the cover frame at intervals, and the upper cover is installed on the cover frame, and the upper cover can be driven to move in a direction perpendicular to the plane where the cover frame is located through the pressing units;

[0009] The locking mechanism is installed on the cabin body, and a plurality of grooves are arranged on the edge of the cover frame. The flip mechanism is clamped on the cabin body by the locking mechanism being inserted into the grooves, and the flip mechanism is located above the chip placement seat.

[0010] Furthermore, the horizontal positioning mechanism includes a positioning push rod and a positioning drive cylinder. A positioning hole is provided on the side wall of the chip placement seat. One end of the positioning push rod is installed on the positioning drive cylinder. The positioning drive cylinder can drive the other end of the positioning push rod to be inserted into the positioning hole or pulled out of the positioning hole.

[0011] Furthermore, the locking mechanism includes a locking plate and a locking drive cylinder, the locking plate is provided with a plurality of protruding locking tongues, the locking plate is installed at the power output end of the locking drive cylinder, and when the flip cover mechanism is in a buckled state, the locking plate is driven to extend by the locking drive cylinder, thereby driving the locking tongue to be stuck in the groove.

[0012] Furthermore, the clamping unit includes a clamping column and a clamping drive cylinder, the power output end of the clamping drive cylinder is installed at one end of the clamping column, and the other end of the clamping column is fixedly connected to the upper cover. The clamping drive cylinder drives the clamping column to move, thereby driving the upper cover to move.

[0013] Furthermore, the flip driving unit includes a flip driving motor and a worm, a worm wheel is provided at the end of the rotating shaft, and the flip driving motor drives the worm to rotate and drives the rotating shaft to rotate through the cooperation of the worm and the worm wheel.

[0014] Furthermore, the air supply and liquid support mechanism is connected to the interior of the cabin through a control valve and a pipeline.

[0015] Furthermore, the chip placement seat is provided with a contoured groove matching the shape of the composite board.

[0016] Furthermore, the chip placement seat is provided with a plurality of through holes, and an inclined seat is provided inside the cabin and below the chip placement seat. The inclined seat is high at both ends and low in the middle, and a strip hole is provided at a lower position of the inclined seat.

[0017] The beneficial effects of the utility model are:

[0018] As an important device in the chip synthesizer, the device of the utility model well solves various requirements in the chip synthesis process, has strong functional integration, and realizes multiple functions such as chip cleaning, supply of common reagents, reagent replacement, reagent cleaning, chip purging, chip drying, etc. required in the chip synthesis process; this device solves the need for the ability to quickly post-process the chip during the chip synthesis process; this device is compatible with the size of the existing standard carrier plate (or synthesis plate) in the biological industry;

[0019] At the same time, it can achieve the effect of great synthetic capacity with minimal reagent consumption, strong flexibility and high functional integration;

[0020] This device innovatively uses gravity to add liquid, achieving the need for rapid and uniform reagent addition on a large-area chip, solving the problems of bubbles, sealing, pressure difference and reagent consumption during the reagent addition process, while the reagent consumption is very low.

[0021] The above description is only an overview of the technical solution of the utility model. In order to more clearly understand the technical means of the utility model and implement it according to the contents of the specification, the following is a detailed description of the preferred embodiments of the utility model in conjunction with the accompanying drawings. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 It is one of the structural schematic diagrams of the utility model;

[0023] Figure 2 This is one of the structural schematic diagrams of the chip placement seat and the flip cover mechanism of the utility model;

[0024] Figure 3 This is the second structural diagram of the chip placement seat and the flip cover mechanism of the utility model (the chip placement seat is not installed);

[0025] Figure 4 This is the second structural diagram of the utility model (the cabin is not shown);

[0026] Figure 5 It is a cross-sectional view of the flip cover mechanism of the utility model (cut along a direction perpendicular to the width of the upper cover);

[0027] Figure 6 This is the third structural diagram of the utility model;

[0028] Figure 7 This is the fourth structural diagram of the utility model;

[0029] The reference numerals are as follows:

[0030] Cabin 1, tilting seat 11, strip hole 111, chip placement seat 2, positioning hole 21, profiling groove 22, through hole 23, flip cover mechanism 3, cover frame 31, groove 311, upper cover 32, upper cover body 311, sealing plate 312, pressing unit 33, pressing column 331, pressing drive cylinder 332, rotating shaft 4, worm gear 41, flip drive unit 5, flip drive motor 51, worm 52, air supply liquid support mechanism 6, locking mechanism 7, locking plate 71, locking tongue 711, locking drive cylinder 72, horizontal positioning mechanism 8, positioning push rod 81, positioning drive cylinder 82;

[0031] Composite board 10. DETAILED DESCRIPTION

[0032] The following is a specific embodiment of the present invention, and those skilled in the art can easily understand the advantages and effects of the present invention from the contents disclosed in this specification. The present invention can also be implemented in other different ways, that is, different modifications and changes can be made without departing from the scope disclosed by the present invention.

[0033] Embodiment: A gravity-fed liquid flip-purge device for a chip synthesizer, such as Figures 1 to 7 As shown, it includes a cabin 1 and a chip placement seat 2 arranged on the cabin, a flip mechanism 3, a rotating shaft 4, a flip driving unit 5, an air supply and liquid support mechanism 6 and a locking mechanism 7. The cabin has an accommodating space inside, and the two ends of the rotating shaft are rotatably connected to the two ends of the cabin. One side of the flip mechanism is sleeved on the rotating shaft, and the flip mechanism can be driven to rotate by the flip driving unit; one side of the chip placement seat is sleeved on the rotating shaft, and the chip placement seat is fixed relative to the cabin by a horizontal positioning mechanism 8, and the horizontal positioning mechanism is installed at the outer wall of the cabin;

[0034] The gas supply liquid support mechanism is installed outside the cabin and can communicate with the interior of the cabin;

[0035] The flip cover mechanism 3 includes a cover frame 31, an upper cover 32 and a plurality of pressing units 33. The pressing units are installed on the cover frame at intervals, and the upper cover is installed on the cover frame. The pressing units can drive the upper cover to move in a direction perpendicular to the plane where the cover frame is located.

[0036] The locking mechanism is installed on the cabin body, and a plurality of grooves 311 are provided on the edge of the cover frame. The locking mechanism is inserted into the grooves to engage the flip mechanism on the cabin body and the flip mechanism is located above the chip placement seat.

[0037] In this embodiment, the horizontal positioning mechanism 8 includes a positioning push rod 81 and a positioning drive cylinder 82. A positioning hole 21 is provided on the side wall of the chip placement seat. One end of the positioning push rod is mounted on the positioning drive cylinder, and the positioning drive cylinder can drive the other end of the positioning push rod to be inserted into or pulled out of the positioning hole. In this embodiment, the horizontal positioning mechanism is provided with two groups, and the positioning holes are provided on both sides of the chip placement seat, and each of the horizontal positioning mechanisms corresponds to one positioning hole.

[0038] In this embodiment, the locking mechanism 7 includes a locking plate 71 and a locking drive cylinder 72. The locking plate is provided with a plurality of protruding lock tongues 711. The locking plate is installed at the power output end of the locking drive cylinder. When the flip mechanism is in the buckled state, the locking plate is driven to extend by the locking drive cylinder, thereby driving the lock tongue to be stuck in the groove. In this embodiment, two locking drive cylinders are provided, which are respectively located at the two ends of the cabin body. The two ends of the locking plate are respectively installed at the power output end of the locking drive cylinder. The lock tongue is driven by the locking drive cylinder to be stuck in the groove or withdrawn from the groove. In this embodiment, a long strip through hole is provided on the side wall of the chip placement seat. The lock tongue passes through the through hole and can be stuck in the groove.

[0039] In this embodiment, the clamping unit 33 includes a clamping column 331 and a clamping drive cylinder 332. The power output end of the clamping drive cylinder is installed at one end of the clamping column, and the other end of the clamping column is fixedly connected to the upper cover. The clamping drive cylinder drives the clamping column to move, thereby driving the upper cover to move.

[0040] In this embodiment, the flip drive unit 5 includes a flip drive motor 51 and a worm 52. A worm wheel 41 is provided at the end of the rotating shaft. The flip drive motor drives the worm to rotate and drives the rotating shaft to rotate through the cooperation of the worm and the worm wheel. The flip drive unit is provided with two groups, which are respectively located at the two ends of the rotating shaft.

[0041] In this embodiment, the air supply liquid support mechanism is connected to the interior of the cabin through a control valve and a pipeline.

[0042] In this embodiment, the chip placement seat is provided with a profiling groove 22 that matches the shape of the composite board 10. The composite board is provided with a plurality of holes. In this embodiment, there are two profiling grooves, two upper covers, and eight clamping units, and every four clamping units correspond to one upper cover, but it is not limited thereto and can be set according to actual conditions.

[0043] In this embodiment, the chip placement seat is provided with a plurality of through holes 23, and an inclined seat 11 is provided inside the chamber and below the chip placement seat. The inclined seat is high at both ends and low in the middle, and a strip hole 111 is provided at a lower position of the inclined seat. The shape design of the inclined seat facilitates the collection and discharge of waste liquid.

[0044] In this embodiment, the upper cover includes a rigid upper cover body 311 and a sealing plate 312 with elastic sealing function (which can be understood as being provided with a circle of sealing ring) to ensure the sealing after buckling.

[0045] The working principle and working process of the utility model are as follows:

[0046] One side of the chip placement seat is fitted onto the shaft and is rotatably connected to the shaft. When the chip placement seat needs to be horizontally positioned and immobilized, the positioning push rod of the horizontal positioning mechanism is inserted into the positioning hole of the cabin body; when it needs to rotate together with the flip mechanism, the positioning push rod of the horizontal positioning mechanism is pulled out from the positioning hole of the cabin body, that is, the horizontal positioning mechanism is unlocked, and the locking mechanism is required to lock the flip mechanism and the chip placement seat together. Since the flip drive unit is driven by a flip drive motor, preferably a servo motor, it can drive the flip mechanism and the chip placement seat to rotate at any angle after linkage, so as to achieve more position requirements.

[0047] This device can have three states. The first is the gravity liquid supply cleaning position (there is a certain angle between the chip placement seat and the flip cover mechanism, such as 90°), where reagents can be added, purged, and cleaned under the action of gravity; the second is the horizontal liquid addition position, the device is closed (the flip cover mechanism is buckled on the top of the chip placement seat), reagents are added, purged, and cleaned; the third is the open position (the chip placement seat and the flip cover mechanism are basically 180°), and chips (or synthetic plates) can be taken and placed, monomers can be added, etc. in this state of the device;

[0048] Different proportions and combinations can be provided according to actual process requirements to achieve the requirements. The following is an example of a process flow. Figure 1 The positioning column of the horizontal positioning mechanism is in the state of being inserted into the positioning hole, so that the chip placement seat is horizontally positioned and locked. In this state, the chip (or synthetic plate) can be placed. Then this set of devices enters the chip main unit and adds monomer reagents through other mechanisms (this part does not belong to the scope of protection of the utility model, so it is not described in detail). After the monomer is completed, exit the main unit; enter Figure 6 The state shown in the figure realizes the closing of the device, and the upper cover moves to the upper side under the push of the flip drive unit. Figure 6The locking drive cylinder drives the locking plate to extend, thereby driving the locking tongue to get into the groove. At the same time, the horizontal positioning mechanism is unlocked (i.e., the positioning push rod is pulled out of the positioning hole). Then the pressing drive cylinder drives the pressing column to exert force on the upper cover, so that the upper cover is tightly buckled above the synthetic plate. At this time, the addition, purge and drying of common reagents in the horizontal position can be realized (this part is realized in conjunction with other mechanisms and does not belong to the scope of protection of the utility model, so it is not described in detail); if there is a need to add reagents vertically and slowly, the flipping drive unit can drive the upper cover 6 to move to a vertical position together with the chip placement seat 4. Figure 7 , this position can be set at any angle, such as 0-150°. The gas supply and liquid support mechanism is connected to the interior of the cabin through the control valve and pipeline, providing the required gas and liquid entry and exhaust gas and liquid for chip synthesis. After completion, it returns to Figure 1 , to realize a small cycle, and then the above devices and steps will be combined according to process requirements.

[0049] The above description is only an embodiment of the present invention, and does not limit the patent scope of the present invention. Any equivalent structure made using the contents of the specification and drawings of the present invention, or directly or indirectly used in other related technical fields, is also included in the patent protection scope of the present invention.

Claims

1. A gravity-fed liquid flipping and purge device for a chip synthesizer, characterized in that: The invention comprises a cabin (1), a chip placement seat (2) arranged on the cabin, a flip mechanism (3), a rotating shaft (4), a flip drive unit (5), an air supply and liquid support mechanism (6) and a locking mechanism (7); the cabin has a storage space inside, two ends of the rotating shaft are rotatably connected to two ends of the cabin, one side of the flip mechanism is mounted on the rotating shaft, and the flip mechanism can be driven to rotate by the flip drive unit; one side of the chip placement seat is mounted on the rotating shaft, and the chip placement seat is fixed relative to the cabin by a horizontal positioning mechanism (8), and the horizontal positioning mechanism is installed on the outer wall of the cabin; The gas supply liquid support mechanism is installed outside the cabin and can communicate with the interior of the cabin; The flip cover mechanism (3) comprises a cover frame (31), an upper cover (32) and a plurality of groups of pressing units (33), wherein the pressing units are installed on the cover frame at intervals, and the upper cover is installed on the cover frame, and the upper cover can be driven to move in a direction perpendicular to the plane where the cover frame is located through the pressing units; The locking mechanism is installed on the cabin body, and a plurality of grooves (311) are provided on the edge of the cover frame. The locking mechanism is inserted into the grooves to engage the flip mechanism on the cabin body, and the flip mechanism is located above the chip placement seat.

2. The gravity-fed liquid-feeding flip-purge device for a chip synthesizer according to claim 1, characterized in that: The horizontal positioning mechanism (8) comprises a positioning push rod (81) and a positioning drive cylinder (82); a positioning hole (21) is provided on the side wall of the chip placement seat; one end of the positioning push rod is mounted on the positioning drive cylinder; the other end of the positioning push rod can be driven by the positioning drive cylinder to be inserted into or pulled out of the positioning hole.

3. The gravity-fed liquid-feeding flip-purge device for a chip synthesizer according to claim 1, characterized in that: The locking mechanism (7) comprises a locking plate (71) and a locking drive cylinder (72), wherein the locking plate is provided with a plurality of protruding locking tongues (711), and the locking plate is mounted on the power output end of the locking drive cylinder. When the flip cover mechanism is in a buckled state, the locking plate is driven to extend by the locking drive cylinder, thereby driving the locking tongue to be stuck in the groove.

4. The gravity-fed liquid-feeding flip-purge device for a chip synthesizer according to claim 1, characterized in that: The clamping unit (33) comprises a clamping column (331) and a clamping drive cylinder (332), wherein a power output end of the clamping drive cylinder is mounted on one end of the clamping column, and the other end of the clamping column is fixedly connected to the upper cover, and the clamping drive cylinder drives the clamping column to move, thereby driving the upper cover to move.

5. The gravity-fed liquid-feeding flip-purge device for a chip synthesizer according to claim 1, characterized in that: The flip drive unit (5) comprises a flip drive motor (51) and a worm (52), a worm wheel (41) is provided at the end of the rotating shaft, and the flip drive motor drives the worm to rotate and drives the rotating shaft to rotate through the cooperation of the worm and the worm wheel.

6. The gravity-fed liquid-feeding flip-purge device for a chip synthesizer according to claim 1, characterized in that: The air supply liquid support mechanism is communicated with the interior of the cabin through a control valve and a pipeline.

7. The gravity-fed liquid-feeding flip-purge device for a chip synthesizer according to claim 1, characterized in that: The chip placement seat is provided with a contoured groove (22) matching the shape of the synthetic board.

8. The gravity-fed liquid-feeding flip-purge device for a chip synthesizer according to claim 1, characterized in that: The chip placement seat is provided with a plurality of through holes (23), and an inclined seat (11) is provided inside the chamber and below the chip placement seat. The inclined seat is high at both ends and low in the middle, and a strip hole (111) is provided at a lower position of the inclined seat.

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