Animal cell culture reaction box with oscillation function

Abstract

The utility model discloses a reaction box with an oscillation function for animal cell culture, which relates to the technical field of cell culture equipment and comprises a reaction box mechanism, the reaction box mechanism comprises a reaction box body, sliding components are mounted at the lower ends of the inner walls of the two sides of the reaction box body, and electric heating tubes are mounted at the bottom ends of the inner walls of the two sides of the sliding components. Protective screen frames fixed to the inner wall of the reaction box body are arranged on the outer sides of the two groups of electric heating pipes in a sleeving manner, a plurality of groups of high-temperature-resistant fans are uniformly fixed to the bottom wall of an inner cavity of the reaction box body, and a spraying assembly extending to the inner cavity of the reaction box body is fixed to the outer wall of one side of the reaction box body. Animal cell culture media are uniformly placed in inner cavities of a second placing hole and a first placing hole, and a pushing and guiding assembly is periodically started to push a motor frame to horizontally move, so that a rotating assembly can drive a lower culture plate and an upper culture plate to synchronously shake after impacting a first vertical plate; a culture solution in the animal cell culture medium can be shaken and shaken up, and the efficiency is relatively high.

Description

Technical Field

[0001] The utility model relates to the technical field of cell culture equipment, in particular to a reaction box with an oscillation function for animal cell culture. Background Art

[0002] Animal cell culture is a technology that takes out relevant tissues from an animal body, disperses them into single cells, and allows these cells to grow and proliferate under suitable culture conditions. This technology is not only the basis of animal cell engineering, but also plays an important role in modern biotechnology. The Chinese utility model patent document with announcement number: CN217516960U discloses a reaction box for animal cell culture, including a box body, a box door is movably installed on the front surface of the box body, a glass plate is clamped inside the box door, a water tank is detachably installed on the upper surface of the box body, a support plate is detachably installed inside the box body, a lighting lamp is fixedly installed on the top wall inside the box body, a control unit is fixedly installed on one side of the box body, a heat dissipation element is fixedly installed on one side of the box body, a mounting frame is detachably installed inside the lower surface of one side of the box body, a filter screen is detachably installed inside the mounting frame, and a windshield is clamped and installed on the outside of the lower surface of one side of the box body.

[0003] When the above utility model patent document is used, since the culture medium is slightly viscous, it is generally necessary to use a manual "cross shaking method" to shake the cells evenly, which takes a long time and is time-consuming and laborious. To this end, we propose a reaction box with a shaking function for animal cell culture to solve the above-mentioned problems. Utility Model Content

[0004] The purpose of this section is to summarize some aspects of the embodiments of the utility model and briefly introduce some preferred embodiments. Some simplifications or omissions may be made in this section and the specification abstract and application title of the utility model to avoid blurring the purpose of this section, specification abstract and application title, and such simplifications or omissions cannot be used to limit the scope of the utility model.

[0005] Therefore, the purpose of the utility model is to provide a reaction box with a shaking function for animal cell culture, which can solve the problem that the existing manual shaking of cells is time-consuming and labor-intensive.

[0006] The utility model provides a reaction box with an oscillation function for animal cell culture, which adopts the following technical scheme:

[0007] A reaction box with an oscillation function for animal cell culture, comprising a reaction box mechanism, which comprises a reaction box body, the lower ends of the inner walls on both sides of the reaction box body are both installed with sliding components, the bottom ends of the inner walls on both sides of the sliding components are both installed with electric heating tubes, the outer sides of two groups of the electric heating tubes are both sleeved with protective mesh frames fixed to the inner wall of the reaction box body, a plurality of groups of high-temperature resistant fans are evenly fixed to the bottom wall of the inner cavity of the reaction box body, a spray component extending into the inner cavity of the reaction box body is fixed to the outer wall of one side of the reaction box body, and the front side wall of the reaction box body is hinged with a switch door body through a hinge;

[0008] A culture mechanism comprises a mounting horizontal plate, wherein the mounting horizontal plate is fixed on the top walls of two groups of sliding components, a first vertical plate and a derivation component are respectively mounted on both sides of the top wall of the mounting horizontal plate, a rotating component is fixed at the end of the derivation component, a lower culture plate is fixed on the top wall of the rotating component, a vertical support component is fixed at the middle position of the top wall of the lower culture plate, an upper culture plate is fixed at the top wall position of the vertical support component, and the top walls of the lower culture plate and the upper culture plate are respectively and evenly provided with a plurality of groups of second placement holes and first placement holes.

[0009] By adopting the above technical solution, the animal cell culture medium is evenly placed in the inner cavity of the second placement hole and the first placement hole, and the derivation component is periodically started to push the motor frame to move horizontally, so that after the rotating component hits the first vertical plate, it can drive the lower culture plate and the upper culture plate to shake synchronously, and then the culture fluid inside the animal cell culture medium can be shaken and evenly distributed, with high efficiency.

[0010] Optionally, the derivation assembly includes a second vertical plate, which is fixed on the top wall of the mounting horizontal plate, a cylinder is fixed on the outer wall of the second vertical plate, and second springs are installed at both upper and lower ends of the inner wall of the second vertical plate.

[0011] Optionally, the rotating assembly includes a motor frame, which is fixed at the end position of the second spring, a driving motor is fixed at the top wall position of the inner cavity of the motor frame, and a rotating shaft fixed to the bottom wall position of the lower culture plate is installed at the output end of the driving motor.

[0012] Optionally, a strip slide is provided on the top wall of the mounting cross plate, and a second slide plate slidably connected to the strip slide is fixed on the bottom wall of the motor frame.

[0013] By adopting the above technical solution, the cylinder is periodically started to expand and contract, thereby pushing the motor frame to move horizontally in the inner cavity of the strip slide through the second slide, so that the motor frame automatically resets under the elastic force of the second spring after hitting the first vertical plate, so that the motor frame periodically impacts and vibrates, thereby driving the lower culture plate and the upper culture plate to shake synchronously, and then the culture fluid inside the animal cell culture medium can be shaken and evenly vibrated, with high efficiency.

[0014] Optionally, the vertical support assembly includes a vertical support tube, which is fixed on the top wall of the lower culture plate. The inner cavity of the vertical support tube is provided with a vertical support column fixed to the bottom wall of the upper culture plate. The outer wall of the vertical support column is provided with a screw hole, and the upper end of the outer wall of the vertical support tube is provided with a bolt matching the thread of the screw hole.

[0015] By adopting the above technical solution, the bolt is screwed to separate it from the screw hole, and then the upper culture plate is moved upward to drive the vertical support column and the vertical support tube to separate, thereby facilitating the removal of the upper culture plate.

[0016] Optionally, a plurality of sets of elastic snap-fit ​​components are fixed at equal angles inside the lower culture plate and the upper culture plate.

[0017] Optionally, the elastic snap-fit ​​assembly includes a transverse tube, which is fixed inside the upper culture plate and the lower culture plate, a first spring is fixed to the inner wall of the transverse tube, a transverse bar passing through the outer wall of the transverse tube is fixed to the end of the first spring, a clamping plate is fixed to the end of the transverse bar, and an anti-slip pad is fixed to the outer wall of the clamping plate.

[0018] By adopting the above technical solution, the animal cell culture medium is evenly placed in the inner cavities of the second placement hole and the first placement hole. Under the elastic force of the first spring, the cross bar can be driven to move synchronously in the inner cavity of the cross tube. Under the action of the cross bar, the clamping plate can be driven to move synchronously, so that the anti-slip pad can cooperate with the first placement hole and the second placement hole to fix the position of the animal cell culture medium, which is convenient for animal cell culture media of different sizes.

[0019] Optionally, the sliding assembly includes a sliding frame, the sliding frame is fixed on the inner wall of the reaction box body, and a first sliding plate fixed to the bottom wall of the mounting horizontal plate is slidably penetrated through the inner cavity of the sliding frame.

[0020] By adopting the above technical solution, the installation cross plate is pulled to drive the first slide plate to slide in the inner cavity of the slide frame, so as to facilitate taking and placing of the animal cell culture medium.

[0021] Optionally, the spray assembly includes a water tank, which is fixed on the outer wall of the reaction box body, and the top wall of the water tank is provided with a pluggable soft plug, a submersible pump is fixed at the bottom wall of the inner cavity of the water tank, and the output end of the submersible pump is equipped with a spray pipe extending into the inner cavity of the reaction box body.

[0022] By adopting the above technical solution, the soft plug is pulled out and an appropriate amount of distilled water is injected into the inner cavity of the water tank. The submersible pump is started to automatically extract the distilled water from the inner cavity of the water tank and evenly atomizes and sprays it onto the animal cell culture medium through the spray pipe, thereby increasing the humidity of the animal cell culture medium.

[0023] In summary, the present invention has at least one of the following beneficial effects:

[0024] The animal cell culture medium is evenly placed in the inner cavity of the second placement hole and the first placement hole, and the cross bar and the clamping plate can be driven to move synchronously under the elastic force of the first spring, so that the anti-slip pad can cooperate with the first placement hole and the second placement hole to fix the position of the animal cell culture medium, which is convenient for animal cell culture media of different sizes;

[0025] The cylinder is periodically activated to extend and retract, thereby pushing the motor frame to move horizontally, so that the motor frame hits the first vertical plate and then automatically resets under the elastic force of the second spring, so that the motor frame periodically hits and vibrates, thereby driving the lower culture plate and the upper culture plate to shake synchronously, and then the culture fluid inside the animal cell culture medium can be shaken and evenly vibrated, with high efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] In order to more clearly illustrate the technical solutions of the embodiments of the utility model, the drawings required for describing the embodiments are briefly introduced below. Obviously, the drawings described below are only some embodiments of the utility model. For ordinary technicians in this field, other drawings can be obtained based on these drawings without creative work.

[0027] Figure 1 This is a schematic diagram of the front cross-sectional structure of the utility model;

[0028] Figure 2 This is a schematic diagram of the front view structure of the utility model;

[0029] Figure 3 This is a schematic diagram of the front cross-sectional structure of the reaction box mechanism of the utility model;

[0030] Figure 4 This is a schematic diagram of the cross-sectional structure of the cultivation mechanism of the utility model;

[0031] Figure 5 This is a schematic diagram of the top view of the upper culture plate of the utility model;

[0032] Figure 6 It is a schematic diagram of the front view and cross-section structure of the rotating assembly and the derivation assembly of the utility model;

[0033] Figure 7 It is a schematic diagram of the front view and cross-section structure of the vertical support assembly of the utility model.

[0034] Explanation of reference numerals: 100, reaction box mechanism; 101, sliding assembly; 1011, first slide plate; 1012, sliding frame; 102, electric heating tube; 103, protective mesh frame; 104, high temperature resistant fan; 105, reaction box body; 106, spray assembly; 1061, submersible pump; 1062, spray pipe fitting; 1063, water tank; 1064, soft plug; 107, switch door body; 200, culture mechanism; 201, upper culture plate; 2011, first placement hole; 202, elastic snap assembly; 2021, cross bar; 2022, first spring; 2023, cross tube ; 2024, clamping plate; 2025, anti-slip pad; 203, lower culture plate; 2031, second placement hole; 204, first vertical plate; 205, mounting horizontal plate; 2051, strip slide; 206, second slide; 207, vertical support assembly; 2071, vertical support column; 20711, screw hole; 2072, bolt; 2073, vertical support tube; 208, rotating assembly; 2081, rotating shaft; 2082, driving motor; 2083, motor frame; 209, derivation assembly; 2091, second spring; 2092, second vertical plate; 2093, cylinder. DETAILED DESCRIPTION

[0035] The following is combined with Figure 1-7 The utility model is described in further detail.

[0036] Example 1, please refer to the attached figure in the specification Figure 1 , 2 3. A reaction box with an oscillation function for animal cell culture, comprising a reaction box mechanism 100, which includes a reaction box body 105, and the lower ends of the inner walls on both sides of the reaction box body 105 are both installed with sliding components 101, and the sliding components 101 include a sliding frame 1012, and the sliding frame 1012 is fixed on the inner wall of the reaction box body 105, and the inner cavity of the sliding frame 1012 is slidably penetrated by a first slide plate 1011. The front side wall of the reaction box body 105 is hinged with a switch door body 107 through a hinge, and the outer wall of the switch door body 107 is installed with a vertical handle and a lock body;

[0037] Please refer to the attached figure in the instruction manual Figure 1 , 4and 5, a culture mechanism 200, which includes a mounting transverse plate 205, which is fixed on the top walls of two groups of first slides 1011, and the first vertical plate 204 and the derivation assembly 209 are respectively mounted on both sides of the top wall of the mounting transverse plate 205, a rotating assembly 208 is fixed at the end of the derivation assembly 209, a lower culture plate 203 is fixed on the top wall of the rotating assembly 208, a vertical support assembly 207 is fixed at the middle position of the top wall of the lower culture plate 203, an upper culture plate 201 is fixed on the top wall position of the vertical support assembly 207, and the top walls of the lower culture plate 203 and the upper culture plate 201 are respectively and evenly provided with a plurality of groups of second placement holes 2031 and first placement holes 2011, and the cross-sections of the second placement holes 2031 and the first placement holes 2011 are both set to be circular.

[0038] Please refer to the attached figure in the instruction manual Figure 5 , multiple groups of elastic snap-fit ​​components 202 are fixed at equal angles inside the lower culture plate 203 and the upper culture plate 201. The elastic snap-fit ​​components 202 include a transverse tube 2023, which is fixed inside the upper culture plate 201 and the lower culture plate 203, a first spring 2022 is fixed to the inner wall of the transverse tube 2023, a transverse bar 2021 penetrating the outer wall of the transverse tube 2023 is fixed to the end of the first spring 2022, a clamping plate 2024 is fixed to the end of the transverse bar 2021, an anti-skid pad 2025 is fixed to the outer wall of the clamping plate 2024, and the cross sections of the clamping plate 2024 and the anti-skid pad 2025 are both set to arc shape.

[0039] The specific working principle is: place the reaction box 105 at an appropriate position, then open the switch door 107 and pull the installation cross plate 205 to the front side to drive the first slide plate 1011 to slide in the inner cavity of the slide frame 1012, and then evenly place the animal cell culture medium in the second placement hole 2031 and the first placement hole 2011. Under the elastic force of the first spring 2022, the cross bar 2021 can be driven to move synchronously in the inner cavity of the cross tube 2023. Under the action of the cross bar 2021, the clamping plate 2024 can be driven to move synchronously, so that the anti-slip pad 2025 can cooperate with the first placement hole 2011 and the second placement hole 2031 to fix the position of the animal cell culture medium, which is convenient for animal cell culture media of different sizes.

[0040] Example 2, please refer to the attached figure in the specification Figure 4 and 6A reaction box with an oscillation function for animal cell culture, comprising: a derivation component 209 including a second vertical plate 2092, the second vertical plate 2092 is fixed on the top wall of the installation horizontal plate 205, a cylinder 2093 is fixed on the outer wall of the second vertical plate 2092, and second springs 2091 are installed at both upper and lower ends of the inner wall of the second vertical plate 2092. A rotating component 208 includes a motor frame 2083, the motor frame 2083 is fixed at the end position of the second spring 2091, a driving motor 2082 is fixed at the top wall position of the inner cavity of the motor frame 2083, and a rotating shaft 2081 fixed to the bottom wall position of the lower culture plate 203 is installed at the output end of the driving motor 2082. A strip slide 2051 is opened on the top wall of the installation horizontal plate 205, and a second slide 206 slidably connected to the strip slide 2051 is fixed on the bottom wall of the motor frame 2083.

[0041] The specific working principle is: after the animal cell culture medium is placed, the cylinder 2093 is periodically started to expand and contract, thereby pushing the motor frame 2083 to move horizontally in the inner cavity of the strip slide 2051 through the second slide 206, so that the motor frame 2083 hits the first vertical plate 204 and then automatically resets under the elastic force of the second spring 2091, so that the motor frame 2083 periodically impacts and vibrates, thereby driving the lower culture plate 203 and the upper culture plate 201 to shake synchronously, and then the culture fluid inside the animal cell culture medium can be shaken and evenly vibrated, which is more efficient.

[0042] Example 3, please refer to the attached figure in the specification Figure 3 A reaction box with an oscillation function for animal cell culture, further comprising: electric heating tubes 102 are installed at the bottom ends of the inner walls on both sides of the sliding component 101, and the outer sides of the two groups of electric heating tubes 102 are sleeved with protective mesh frames 103 fixed to the inner wall of the reaction box body 105, and multiple groups of high-temperature resistant fans 104 are evenly fixed on the bottom wall of the inner cavity of the reaction box body 105. The protective mesh frames 103 are arranged at the outer sides of the multiple groups of high-temperature resistant fans 104, and the protective mesh frames 103 and the high-temperature resistant fans 104 are both arranged at the lower side of the installation horizontal plate 205.

[0043] The specific working principle is: when the temperature of the inner cavity of the reaction box 105 is too low, the electric heating tube 102 is started and energized to generate heat, thereby heating the air in the inner cavity of the reaction box 105, and multiple groups of high-temperature resistant fans 104 are started and energized to generate air flow, thereby increasing the air flow rate in the inner cavity of the reaction box 105, thereby making the temperature distribution in the inner cavity of the reaction box 105 uniform, making it convenient to heat up the animal cell culture fluid in the inner cavity of the reaction box 105 to prevent it from losing its activity due to excessive low temperature.

[0044] Example 4, please refer to the attached figure in the specification Figure 3A reaction box with an oscillation function for animal cell culture also includes a spray assembly 106 fixed to the outer wall of one side of the reaction box body 105, the spray assembly 106 includes a water tank 1063, the water tank 1063 is fixed on the outer wall of the reaction box body 105, a pluggable soft plug 1064 is provided on the top wall of the water tank 1063, a submersible pump 1061 is fixed to the bottom wall of the inner cavity of the water tank 1063, a spray pipe 1062 extending to the inner cavity of the reaction box body 105 is installed at the output end of the submersible pump 1061, and a nozzle is installed at the end of the spray pipe 1062, and the nozzle is arranged directly above the second placement hole 2031 and the first placement hole 2011.

[0045] The specific working principle is: pull out the soft plug 1064 and inject an appropriate amount of distilled water into the inner cavity of the water tank 1063, start the submersible pump 1061 to automatically extract the distilled water from the inner cavity of the water tank 1063 and evenly atomize and spray it onto the animal cell culture medium through the spray pipe 1062, start the drive motor 2082 to drive the rotating shaft 2081 to rotate, thereby driving the lower culture plate 203 and the upper culture plate 201 to rotate synchronously, thereby increasing the humidity of the animal cell culture medium at different positions.

[0046] Example 5, please refer to the attached figure in the specification Figure 4 and 7 A reaction box with an oscillation function for animal cell culture also includes a vertical support assembly 207 including a vertical support tube 2073, the vertical support tube 2073 is fixed on the top wall of the lower culture plate 203, a vertical support column 2071 fixed to the bottom wall of the upper culture plate 201 is passed through the inner cavity of the vertical support tube 2073, a screw hole 20711 is opened on the outer wall of the vertical support column 2071, and a bolt 2072 matching the thread of the screw hole 20711 is passed through the upper end of the outer wall of the vertical support tube 2073.

[0047] The specific working principle is: screw the bolt 2072 to separate it from the screw hole 20711, and then move the upper culture plate 201 upward to drive the vertical support column 2071 to separate from the vertical support tube 2073, so as to facilitate the disassembly of the upper culture plate 201.

[0048] The standard parts used in the present utility model document can all be purchased from the market. The various components in the present utility model document can be customized according to the description in the specification and the drawings. The specific connection methods of each part adopt conventional means such as mature bolts, rivets, welding, etc. in the prior art. The machinery, parts and equipment all adopt conventional models in the prior art, and the circuit connection adopts the conventional connection method in the prior art, which will not be described in detail here.

[0049] The above are all preferred embodiments of the present utility model, and are not intended to limit the protection scope of the present utility model. Therefore, any equivalent changes made based on the structure, shape, and principle of the present utility model should be included in the protection scope of the present utility model.

Claims

1. A reaction box with a shaking function for animal cell culture, characterized in that: include, A reaction box mechanism (100) comprises a reaction box body (105), wherein the lower ends of the inner walls on both sides of the reaction box body (105) are both installed with sliding components (101), the lower ends of the inner walls on both sides of the sliding components (101) are both installed with electric heating tubes (102), the outer sides of the two groups of electric heating tubes (102) are both sleeved with protective net frames (103) fixed to the inner walls of the reaction box body (105), a plurality of groups of high temperature resistant fans (104) are evenly fixed to the bottom wall of the inner cavity of the reaction box body (105), a spray component (106) extending into the inner cavity of the reaction box body (105) is fixed to the outer wall of one side of the reaction box body (105), and the front side wall of the reaction box body (105) is hinged with a switch door (107) via a hinge; A culture mechanism (200) comprises a mounting cross plate (205), wherein the mounting cross plate (205) is fixed on the top walls of two groups of sliding components (101), a first vertical plate (204) and a derivation component (209) are respectively mounted on both sides of the top wall of the mounting cross plate (205), a rotating component (208) is fixed at the end of the derivation component (209), a lower culture plate (203) is fixed on the top wall of the rotating component (208), a vertical support component (207) is fixed at the middle position of the top wall of the lower culture plate (203), an upper culture plate (201) is fixed on the top wall of the vertical support component (207), and a plurality of groups of second placement holes (2031) and first placement holes (2011) are respectively and evenly provided on the top walls of the lower culture plate (203) and the upper culture plate (201).

2. The reaction box with shaking function for animal cell culture according to claim 1, characterized in that: The derivation assembly (209) comprises a second vertical plate (2092), the second vertical plate (2092) being fixed on the top wall of the mounting horizontal plate (205), a cylinder (2093) being fixed on the outer wall of the second vertical plate (2092), and second springs (2091) being installed on both upper and lower ends of the inner wall of the second vertical plate (2092).

3. The reaction box with shaking function for animal cell culture according to claim 2, characterized in that: The rotating assembly (208) comprises a motor frame (2083), wherein the motor frame (2083) is fixed at the end position of the second spring (2091), a driving motor (2082) is fixed at the top wall position of the inner cavity of the motor frame (2083), and a rotating shaft (2081) fixed to the bottom wall position of the lower culture plate (203) is installed at the output end of the driving motor (2082).

4. The reaction box with shaking function for animal cell culture according to claim 1, characterized in that: The vertical support assembly (207) comprises a vertical support tube (2073), wherein the vertical support tube (2073) is fixed on the top wall of the lower culture plate (203), the inner cavity of the vertical support tube (2073) is provided with a vertical support column (2071) fixed to the bottom wall of the upper culture plate (201), the outer wall of the vertical support column (2071) is provided with a screw hole (20711), and the upper end of the outer wall of the vertical support tube (2073) is provided with a bolt (2072) that matches the thread of the screw hole (20711).

5. The reaction box with shaking function for animal cell culture according to claim 1, characterized in that: Multiple groups of elastic snap-fit ​​components (202) are fixed at equal angles inside the lower culture plate (203) and the upper culture plate (201).

6. The reaction box with shaking function for animal cell culture according to claim 5, characterized in that: The elastic snap-fit ​​assembly (202) comprises a transverse tube (2023), wherein the transverse tube (2023) is fixed inside the upper culture plate (201) and the lower culture plate (203), a first spring (2022) is fixed to the inner wall of the transverse tube (2023), a transverse bar (2021) penetrating the outer wall of the transverse tube (2023) is fixed to the end of the first spring (2022), a clamping plate (2024) is fixed to the end of the transverse bar (2021), and an anti-slip pad (2025) is fixed to the outer wall of the clamping plate (2024).

7. The reaction box with shaking function for animal cell culture according to claim 1, characterized in that: The sliding assembly (101) comprises a sliding frame (1012), wherein the sliding frame (1012) is fixed on the inner wall of the reaction box (105), and a first sliding plate (1011) is slidably penetrated through the inner cavity of the sliding frame (1012) and fixed to the bottom wall of the mounting horizontal plate (205).

8. The reaction box with shaking function for animal cell culture according to claim 3, characterized in that: The top wall of the mounting transverse plate (205) is provided with a strip slideway (2051), and the bottom wall of the motor frame (2083) is fixed with a second slide plate (206) slidably connected to the strip slideway (2051).

9. The reaction box with shaking function for animal cell culture according to claim 1, characterized in that: The spray assembly (106) comprises a water tank (1063), the water tank (1063) being fixed on the outer wall of the reaction box body (105), the top wall of the water tank (1063) being provided with a pluggable soft plug (1064), a submersible pump (1061) being fixed at the bottom wall of the inner cavity of the water tank (1063), and a spray pipe (1062) extending into the inner cavity of the reaction box body (105) being installed at the output end of the submersible pump (1061).

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