Multi-channel nucleic acid extractor

Abstract

The utility model provides a multichannel nucleic acid extractor, including chassis subassembly, magnetic separation drive module, magnetic separation module and incubation module, chassis subassembly includes bottom plate and first slide rail, the magnetic separation drive module is fixed on the bottom plate, incubation module is in slip connection with first slide rail, the magnetic separation drive module is fixed on the first slide rail, the magnetic separation drive module is fixed on the first slide rail, and the incubation module is fixed on the first slide rail. The magnetic separation driving module is arranged on the first sliding rail and moves in the length direction of the first sliding rail, and the first sliding rail faces the magnetic separation driving module. The whole extractor is composed of the base plate assembly, the magnetic separation driving module, the magnetic separation module and the incubation module, complete extraction work can be carried out, all the parts are reasonably arranged in different modules in a classified mode through functions, extraction work of multiple sets of samples is achieved, the overall complexity of the extractor is simplified, and meanwhile the extraction efficiency is improved. The extraction efficiency is greatly improved.

Description

Technical Field

[0001] The utility model belongs to the field of medical instruments, and specifically relates to a multi-channel nucleic acid extraction instrument. Background Art

[0002] The nucleic acid extractor is an instrument that uses matching nucleic acid extraction reagents to automatically complete the sample nucleic acid extraction work. It is widely used in various fields such as disease control centers, clinical disease diagnosis, blood transfusion safety, forensic identification, environmental microbiology testing, food safety testing, animal husbandry and molecular biology research. Standard nucleic acid extraction includes the following basic steps: 1. Lysis: Add lysis solution to the sample, and achieve mixing and full reaction of the reaction solution through mechanical movement and heating to lyse the cells and release nucleic acids; 2. Adsorption: Add magnetic beads to the sample lysis solution, mix thoroughly, and use the characteristics of magnetic beads with strong affinity for nucleic acids under high salt and low pH to adsorb nucleic acids, and separate the magnetic beads from the lysis solution under the action of an external magnetic field; 3. Washing: Transfer the magnetic beads adsorbed with nucleic acids to a new washing buffer and mix thoroughly to wash away impurities, and then separate the magnetic beads from the washing buffer under the action of an external magnetic field; 4. Elution: Transfer the magnetic beads to the elution buffer, remove the external magnetic field, and mix the magnetic beads and the elution solution thoroughly. The bound nucleic acids can be separated from the magnetic beads and mixed into the elution buffer, thereby obtaining purified nucleic acids. The entire extraction process is cumbersome, and the current methods of performing nucleic acid extraction are mainly manual extraction and machine extraction. Manual extraction consumes a lot of human resources, and for machine extraction, although there are some machine-extracted nucleic acid extraction instrument products on the market, due to the cumbersome extraction steps, the structures of these products are very complicated, and the coordination of various parts is inefficient, making the average time for a complete extraction about half an hour, which is very time-consuming and difficult to reduce. Therefore, the efficiency is very low and difficult to improve. Utility Model Content

[0003] The utility model aims to solve the deficiencies of the prior art and provides a multi-channel nucleic acid extraction instrument.

[0004] The technical solution adopted by the utility model is as follows:

[0005] A multi-channel nucleic acid extractor comprises a chassis assembly, a magnetic separation drive module, a magnetic separation module and an incubation module, wherein the chassis assembly comprises a bottom plate and a first slide rail, the magnetic separation drive module is fixedly arranged on the bottom plate, the incubation module is slidably connected to the first slide rail and moves along the length direction of the first slide rail, and the direction of the first slide rail is arranged to be toward the magnetic separation drive module; the magnetic separation drive module is provided with a second slide rail arranged vertically, the magnetic separation module is slidably connected to the second slide rail and moves up and down along the second slide rail; the incubation module is provided with eight rows of reagent tubes, and the magnetic separation module is provided with a magnetic rod for extending downward into the eight rows of reagent tubes.

[0006] Furthermore, the number of the eight-row reagent tubes is set to four rows, and they are arranged in an array parallel to each other. The eight-row reagent tubes include eight grids, and the grids are arranged in an array along the straight direction of the eight-row reagent tubes. The number of the magnetic rods is set to four, and they are used to extend into four of the eight-row reagent tubes respectively.

[0007] Furthermore, the magnetic separation module also includes a guide shaft, a magnetic rod fixing block, and a screw motor. The magnetic rod fixing block is axially sleeved on the guide shaft, and the output shaft of the screw motor is threadedly connected to the magnetic rod fixing block for driving the magnetic rod fixing block to rise and fall. The magnetic rod is connected to the bottom of the magnetic rod fixing block.

[0008] Furthermore, the incubation module includes a fixed bottom plate, a test tube fixing block is arranged on the fixed bottom plate, the eight-row reagent tubes are installed on the test tube fixing block, and a heating plate for heating is arranged in the test tube fixing block.

[0009] Furthermore, a rubber-coated roller is provided on one side of the fixed bottom plate, the rubber-coated roller abuts against the bottom plate and rolls relative to the bottom plate when the incubation module moves.

[0010] Furthermore, the magnetic separation driving module includes a magnetic separation driving fixed sheet metal, the second slide rail is arranged on the magnetic separation driving fixed sheet metal, and also includes a second belt for driving the magnetic separation module to rise and fall.

[0011] Furthermore, the bottom of the magnetic separation drive fixing sheet metal is provided with an opening for the incubation module to pass through.

[0012] Furthermore, a first belt for driving the incubation module to move is provided at the bottom of the base plate.

[0013] Furthermore, bakelite is provided at the bottom of the fixed base plate.

[0014] The beneficial effect of the utility model lies in that the entire extractor is composed of four parts: a chassis assembly, a magnetic separation drive module, a magnetic separation module and an incubation module, so as to be able to carry out complete extraction work. The functions of each part are classified and reasonably arranged in different modules, and the reagent tubes are loaded on the incubation module to perform the heating and lysis function, and the movement of the reagent tubes is realized with the base slide rail setting. In conjunction with the setting of the magnetic rod on the magnetic separation module and the movement of the magnetic rod provided by the slide rail, the multiple flexibility ensures that when multiple groups of reagent tubes and multiple magnetic rods are set, each magnetic rod can fully interact with each reagent tube to realize the extraction of multiple groups of samples. While simplifying the overall complexity of the device, the extraction efficiency is greatly improved. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 The overall structure diagram of the nucleic acid extraction instrument provided by the utility model;

[0016] Figure 2 The structure diagram of the chassis assembly provided by the utility model Figure 1 ;

[0017] Figure 3 The structure diagram of the chassis assembly provided by the utility model Figure 2 ;

[0018] Figure 4 The structure of the incubation module provided by the utility model is shown in FIG. Figure 1 ;

[0019] Figure 5 The structure of the incubation module provided by the utility model is shown in FIG. Figure 2 ;

[0020] Figure 6 The structure diagram of the magnetic separation drive module provided by the utility model Figure 1 ;

[0021] Figure 7 The structure diagram of the magnetic separation drive module provided by the utility model Figure 2 ;

[0022] Figure 8 The structure diagram of the magnetic separation module provided by the utility model Figure 1 ;

[0023] Fig. 9 The structure diagram of the magnetic separation module provided by the utility model Figure 2 ;

[0024] In the figure: 1-chassis assembly, 11-rear plate, 110-first stepper motor, 111-control board, 112-stepper controller, 12-drag chain, 13-first belt, 14-first synchronous pulley, 15-USB connector, 16-button switch, 17-first slide rail, 18-first optical coupler,

[0025] 2-incubation module, 21-row reagent tubes, 22-heating plate, 23-test tube fixing block, 24-drag chain fixing sheet metal, 25-pulley fixing sheet metal, 26-bakelite, 27-first slider, 28-fixed bottom plate, 29-rubber-coated roller,

[0026] 3-magnetic separation module, 31-magnetic bar fixing block, 32-guide shaft, 33-screw nut, 34-magnetic bar, 35-linear bearing, 36-second slider, 37-magnetic separation fixing frame, 38-synchronous belt fixing plate, 39-screw motor,

[0027] 4-magnetic separation drive module, 41-magnetic separation drive fixing sheet metal, 42-second optical coupler, 43-second slide rail, 44-motor fixing bracket, 45-second stepping motor, 46-power supply. DETAILED DESCRIPTION

[0028] In order to describe the utility model more specifically, the technical solution of the utility model is described in detail below in conjunction with the accompanying drawings and specific implementation methods. These descriptions only show how the utility model is implemented and cannot limit the specific scope of the utility model. The scope of the utility model is defined in the claims.

[0029] like Figure 1 As shown, this embodiment provides a multi-channel nucleic acid extraction instrument, including a chassis assembly 1, on which a magnetic separation drive module 4 is installed, on which a magnetic separation module 3 is installed, and the chassis assembly 1 is also equipped with an incubation module 2.

[0030] Specifically, refer to Figure 2 and Figure 3 The chassis assembly 1 includes a bottom plate 19 and a back plate 11. A control board 111 is installed at the front end of the back plate 11. The control board 111 is electrically connected to the incubation module 2, the magnetic separation module 3, and the magnetic separation drive module 4, and receives and sends instructions to control the actions of each module. A step controller 112, a USB connection port 15, a filter and a button switch 16 are also installed on the back plate 11. The button switch 16 is used to control the opening and closing of the multi-channel nucleic acid extractor.

[0031] The bottom plate 19 is provided with a first slide rail 17. The number of the first slide rails 17 is set to two and they are arranged parallel to each other. Figure 5The incubation module 2 includes a fixed bottom plate 28, and a first slider 27 is arranged at the bottom of the fixed bottom plate 28, so that the first slide rail 17 can slide with the first slider 27, so that the incubation module 2 can be installed on the bottom plate 19 and can perform a translational movement along the direction of the first slide rail 17, and a first optical coupler 18 is arranged at one end of the first slide rail 17 for confirming the relative position of the incubation module 2 on the first slide rail 17.

[0032] In order to drive the incubation module 2, two first synchronous pulleys 14 arranged along the length direction of the first slide rail 17 are provided at the bottom of the base plate 19, and the first belt 13 is commonly sleeved on the two first synchronous pulleys 14. The incubation module 2 includes a fixed base plate 28, and a pulley fixing sheet metal 25 is provided at the bottom of the fixed base plate 28. A strip groove is connected on the base plate 19, so that the pulley fixing sheet metal 25 can pass downward through the strip groove and be connected to the first belt 13. A first stepper motor 110 is also provided on the base plate 19, which is used to control the rotation of the first synchronous pulley 14 to control the operation of the first belt 13, thereby driving the incubation module 2 to move on the base plate 19.

[0033] In addition, in order to facilitate wiring, improve the stability and smoothness of the movement of the incubation module 2, and avoid jitter during translation as much as possible, in this embodiment, a drag chain 12 is further arranged at the bottom of the base plate 19, and a drag chain fixing sheet metal 24 is arranged at the bottom of the fixed base plate 28. A corresponding strip groove is opened on the base plate 19 for the drag chain fixing sheet metal 24 to pass downward, so that the drag chain fixing sheet metal 24 is connected to the drag chain 12, and then when the incubation module 2 is translated, it is stabilized by the drag chain 12; a rubber-coated roller 29 is also arranged on the front part of the base plate, and the rubber-coated roller 29 is against the base plate 19 and rolls relative to the base plate 19 when the incubation module 2 moves. The rubber-coated material on the surface of the rubber-coated roller 29 can effectively fit with the surface of the base plate 19, reduce bouncing during movement, and absorb vibration, so as to effectively ensure the stability and smooth movement of the incubation module.

[0034] For the incubation module 2, refer to Figure 4A test tube fixing block 23 is installed on the fixed bottom plate 28, and four columns of eight-row reagent tubes 21 are arranged on the test tube fixing block 23. Each column of the eight-row reagent tubes 21 includes eight holes, and the eight holes are arranged in an array along a straight line direction. The straight line direction is the length direction of the first slide rail 17, and the four columns of eight-row reagent tubes 21 are arranged in an array parallel to each other in a direction perpendicular to the straight line direction; a heating plate 22 is also provided inside the test tube fixing block 23 for heating the eight-row reagent tubes 21 to promote cracking, and bakelite 26 is also provided at the bottom of the bottom plate 19 for heat insulation. On the one hand, the heat energy is maintained in the test tube fixing block 23 to ensure the heating effect, and on the other hand, the heat is prevented from diffusing to the bottom and affecting other components.

[0035] The magnetic separation drive module 4 is fixedly mounted on the bottom plate 19. Figure 6 and Figure 7 The magnetic separation drive module 4 includes a magnetic separation drive fixed sheet metal 41, the bottom of the magnetic separation drive fixed sheet metal 41 is fixedly connected to the bottom plate 19, and two second slide rails 43 parallel to each other are arranged thereon, and the length direction of the second slide rail 43 is arranged vertically, and the back side of the magnetic separation drive fixed sheet metal 41 is provided with two second synchronous pulleys arranged along the length direction of the second slide rail 18, and the two second synchronous pulleys are jointly sleeved with a second belt, and a second stepping motor 45 is also provided for driving the rotation of the second synchronous pulley to drive the operation of the second belt, and a power supply 46 is also provided for powering the second stepping motor 45.

[0036] Reference Figure 8 and Fig. 9 The magnetic separation module 3 includes a magnetic separation fixing frame 37, and two second sliders 36 are respectively arranged on the back side of the magnetic separation fixing frame 37, which are opposite to the second slide rail 18 respectively. The second slide rail 18 can be slidably engaged with the second slider 36, so that the magnetic separation module 3 can be installed on the magnetic separation drive fixing sheet metal 41 and can be moved vertically along the direction of the second slide rail 18, and a second optical coupler 42 is arranged at one end of the second slide rail 18 for confirming the relative position of the magnetic separation module 3 on the second slide rail 18.

[0037] In addition, a synchronous belt fixing plate 38 is also provided on the back side of the magnetic separation fixing frame 37, and a connecting through groove is opened on the magnetic separation drive fixing sheet metal 41. The synchronous belt fixing plate 38 can pass through the through groove to connect with the second belt, and then the operation of the second belt can drive the synchronous belt fixing plate 38 to move, thereby driving the movement of the magnetic separation module 3.

[0038] A guide shaft 32 is provided on the front side of the magnetic separation fixing frame 37. The guide shaft 32 is arranged in the vertical direction and is provided with two parallel to each other. A magnetic rod fixing block 31 is jointly sleeved on the two guide shafts 32. The magnetic rod fixing block 31 can slide along the axial direction of the guide shaft 32, that is, move in the vertical direction. A screw motor 39 is provided on the magnetic separation fixing frame 37. The output shaft of the screw motor 39 is provided with a vertically downward screw. A screw nut 33 is threaded on the screw. The screw nut 33 is fixedly connected to the magnetic rod fixing block 31, and when the screw motor 39 is running, the magnetic rod fixing block 31 can be driven to move up and down through the screw nut 33.

[0039] A magnetic rod 34 is arranged at the bottom of the magnetic rod fixing block 31, and a hole is opened on the magnetic separation fixing frame 37 for the magnetic rod 34 to pass downward. In this embodiment, four magnetic rods 34 are arranged, and the four magnetic rods 34 are arranged along the arrangement direction and arrangement spacing between the four columns of the eight-row reagent tubes 21, so that the four magnetic rods 34 can correspond one by one to the four columns of the eight-row reagent tubes 21 respectively.

[0040] The length direction of the first slide rail 17 in this embodiment is set to be the direction of approaching or moving away from the magnetic separation module 3, and the bottom of the magnetic separation drive fixed sheet metal 41 is provided with an opening for the incubation module 2 to pass through, that is, when the incubation module 2 moves along the first slide rail 17, it can move to the bottom of the magnetic separation module 3 and adjust the position by moving forward and backward, so that the four magnetic rods 34 can be simultaneously inserted into the holes in the same row of four columns and eight rows of reagent tubes 21 when moving downward.

[0041] In this embodiment, in each column of the eight-row reagent tubes 21, the insertion and extraction order of the magnetic rod 34 can be set to insert the magnetic rod in the order of the first well-the third well-the second well-the fourth well-the fifth well-the seventh well-the second well-the first well along the length direction of the eight-row reagent tubes, and the extraction steps in each insertion in the corresponding order are magnetic sleeve installation-magnetic absorption-lysis-washing-washing-elution-magnetic loss-magnetic removal.

[0042] In this embodiment, four magnetic bars are arranged corresponding to four columns and eight rows of reagent tubes, so that nucleic acid can be extracted from four samples at the same time, which greatly reduces the extraction time and improves the extraction efficiency. Compared with traditional nucleic acid extractors, the nucleic acid extractor provided in this embodiment has high extraction efficiency and automation, and can extract nucleic acid from multiple reagent tubes at the same time, which is particularly suitable for the rapid extraction of small batches of nucleic acid. At the same time, the instrument is small in size and easy to transport and store.

[0043] Although the utility model is disclosed as above, the utility model is not limited thereto. Any person skilled in the art can make various changes and modifications without departing from the spirit and scope of the utility model. Therefore, the protection scope of the utility model shall be subject to the scope defined by the claims.

Claims

1. A multi-channel nucleic acid extraction instrument, characterized in that: It includes a chassis assembly, a magnetic separation drive module, a magnetic separation module and an incubation module, the chassis assembly includes a base plate and a first slide rail, the magnetic separation drive module is fixedly arranged on the base plate, the incubation module is slidably connected to the first slide rail, and moves along the length direction of the first slide rail, and the direction of the first slide rail is set to face the magnetic separation drive module; the magnetic separation drive module is provided with a second slide rail arranged vertically, the magnetic separation module is slidably connected to the second slide rail, and moves up and down along the second slide rail; the incubation module is provided with eight rows of reagent tubes, and the magnetic separation module is provided with a magnetic rod for extending downward into the eight rows of reagent tubes.

2. The multi-channel nucleic acid extractor according to claim 1, characterized in that: The number of the eight-row reagent tubes is set to four rows, and they are arranged in a parallel array. The eight-row reagent tubes include eight wells, and the wells are arranged in an array along the straight direction of the eight-row reagent tubes. The number of the magnetic rods is set to four, and they are used to extend into four of the eight-row reagent tubes respectively.

3. The multi-channel nucleic acid extractor according to claim 1 or 2, characterized in that: The magnetic separation module also includes a guide shaft, a magnetic rod fixing block, and a screw motor. The magnetic rod fixing block is axially sleeved on the guide shaft. The output shaft of the screw motor is threadedly connected to the magnetic rod fixing block for driving the magnetic rod fixing block to rise and fall. The magnetic rod is connected to the bottom of the magnetic rod fixing block.

4. The multi-channel nucleic acid extractor according to claim 1, characterized in that: The incubation module comprises a fixed bottom plate, a test tube fixing block is arranged on the fixed bottom plate, the eight-row reagent tubes are installed on the test tube fixing block, and a heating plate for heating is arranged in the test tube fixing block.

5. The multi-channel nucleic acid extraction instrument according to claim 4, characterized in that: A rubber-coated roller is also provided on one side of the fixed bottom plate, and the rubber-coated roller abuts against the bottom plate and rolls relative to the bottom plate when the incubation module moves.

6. The multi-channel nucleic acid extraction instrument according to claim 1, characterized in that: The magnetic separation driving module includes a magnetic separation driving fixed sheet metal, the second slide rail is arranged on the magnetic separation driving fixed sheet metal, and also includes a second belt for driving the magnetic separation module to rise and fall.

7. The multi-channel nucleic acid extraction instrument according to claim 6, characterized in that: The bottom of the magnetic separation drive fixing sheet metal is provided with an opening for the incubation module to pass through.

8. The multi-channel nucleic acid extraction instrument according to claim 1, characterized in that: A first belt for driving the incubation module to move is provided at the bottom of the base plate.

9. The multi-channel nucleic acid extraction instrument according to claim 4, characterized in that: The bottom of the fixed bottom plate is provided with bakelite.

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