Dental pulp stem cell quantitative analysis device

Abstract

The utility model discloses a dental pulp stem cell quantitative analysis device which comprises a base, a microscope and a controller are fixedly installed on the top face of the base, a detection mechanism is further arranged on the base, and the detection mechanism comprises a placing assembly, a lifting assembly and a feeding assembly. By arranging the limiting frames and the connecting rods, different specifications and sizes can be conveniently fixed, during use, a pressing plate is pressed to push a top plate to move through the connecting rods, then the top plate drives the limiting frames to move to enlarge the distance between the limiting frames, and then a slide frame for storing slides is placed on a partition plate and located between the limiting frames; at the moment, the pressing plate is loosened, the limiting frame can be driven by the elastic force of the fixing box to enable the slide rack to be located in the middle of the partition plate to be positioned, the slide rack positioning device can adapt to slide racks of different specifications to position the slide racks, and convenience and rapidness are achieved.

Description

Technical Field

[0001] The utility model relates to the technical field of detection and analysis instruments, in particular to a dental pulp stem cell quantitative analysis device. Background Art

[0002] Dental pulp stem cells are adult stem cells isolated from dental pulp tissue with self-renewal and multidirectional differentiation potentials. Quantitative analysis of dental pulp stem cells usually involves the measurement and analysis of the number, activity, differentiation potential, and specific protein expression of dental pulp stem cells.

[0003] Referring to the fully automatic cell DNA quantitative analysis equipment disclosed in the patent application with publication number CN207557262U, the glass slide device and the detection device are respectively fixedly mounted on the bottom plate, and the glass slide device is provided with a shell and a door mounted on the shell, and the moving device is fixedly connected to the detection device. The effects of the utility model are: 1. The glass slide device automatically feeds, automatically detects and automatically prints data, and has high detection efficiency and automation; 2. The multi-station fixture is used to detect samples, which is more convenient for operation and batch detection.

[0004] However, the above-mentioned technical device uses a moving device to drive the glass slide device to feed the material, and the glass slide device uses a spacer to fix the position of the glass slide. When the size of the glass slide changes, it is often necessary to replace the entire glass slide plate to adapt to the new glass slide specifications. For example, when the size of the glass slide changes, the glass slide plate on which the glass slide is placed needs to be replaced, and the above-mentioned device is not convenient for fixing glass slide plates of different specifications, and its practicality is general. Utility Model Content

[0005] In view of the deficiencies of the prior art, the utility model provides a dental pulp stem cell quantitative analysis device, which solves the problem that the prior cell quantitative analysis device is inconvenient to fix glass slides of different specifications and has general practicality.

[0006] To achieve the above objectives, the utility model is implemented through the following technical solutions: a dental pulp stem cell quantitative analysis device, comprising a base, a microscope and a controller are fixedly mounted on the top surface of the base, and a detection mechanism is also arranged on the top surface of the base, and the detection mechanism includes:

[0007] A placement assembly comprises a fixed box fixedly arranged on the left side of the base, a placement box movably arranged in the fixed box, a placement rack movably arranged in the placement box, a plurality of partitions movably arranged in the placement rack, and a glass slide rack placed on the partition;

[0008] A lifting component is arranged inside the fixed box, and the lifting component is connected to the placement box and the controller respectively, and is used to adjust the height of the placement box;

[0009] The feeding assembly is arranged on the right side of the fixed box, connected to the controller, and communicated with the inside of the fixed box, and is used to drive the glass slide in the fixed box to move to the bottom of the microscope to quantitatively analyze the dental pulp stem cells on the glass slide.

[0010] Preferably, a movable groove is provided at the inner bottom of the placement rack, four limit racks are movably provided in the movable groove, four limit racks are provided on the placement rack and the partition plate, the limit racks are correspondingly inserted into the limit racks, a spring is fixedly connected between the lower end of the limit rack and the movable groove, and a connecting component is movably installed on the top surface of the placement rack.

[0011] Preferably, the connection assembly comprises:

[0012] Two top plates, two top plates are arranged above the placement rack, the two top plates are arranged in parallel at the top of the limiting rack, and the two top plates are perpendicular to the setting direction of the spring;

[0013] A connecting rod, wherein each of the top plates is movably hinged with a connecting rod in the middle;

[0014] A pressure plate is movably hinged at the top ends of the two connecting rods.

[0015] Preferably, the lifting assembly comprises:

[0016] A slide slot is provided inside the rear side wall of the fixed box;

[0017] A first screw rod is rotatably disposed in the slide groove;

[0018] A first motor is fixedly arranged at the top of the slide slot, the first motor is connected to the controller, and the output shaft of the first motor is coaxially fixedly connected to the upper end of the first screw;

[0019] A slider is fixedly arranged at the rear side of the placement box, and the slider is slidably embedded in the slide groove, and the first screw rod penetrates and threadably engages with the slider;

[0020] The laser locator is fixedly arranged on the right side wall of the inner cavity of the fixed box and is connected to the controller.

[0021] Preferably, the feeding assembly comprises:

[0022] A notch is provided on the right side of the fixed box;

[0023] A fixed frame is fixedly arranged on the right side of the fixed box, a connecting plate is movably arranged in the fixed frame, a mounting groove is arranged on the bottom surface of the fixed frame, and a connecting groove is arranged on the front side of the fixed frame;

[0024] A second screw rod is movably arranged inside the connecting groove, a second motor is fixedly arranged on the right side of the fixing frame, the second motor is connected to the controller, an output shaft of the second motor is coaxially fixedly connected to the second screw rod, and the second screw rod penetrates and is threadedly engaged with one end of the connecting plate;

[0025] The card plate is fixedly arranged on the left side of the connecting plate. A card slot is opened on the right side of the upper surface of the glass slide rack. The card plate is used to movably pass through the slot and be clamped in the card slot.

[0026] The working principle of the utility model is as follows: when in use, the user places the glass slide on the glass slide rack, and then presses the pressure plate to push the top plate to move through the connecting rod, and then the top plate will drive the limit frame to move to expand the distance between the limit frames, and then the glass slide rack is placed on the partition so that it is located between the limit frames, then the pressure plate is released, and the limit frame is driven to reset through the elastic force of the spring, so that the glass slide rack is located in the middle of the partition and fixed in position, and then the placement rack is picked up and placed inside the placement box, and the position of the glass slide rack can be detected by the laser locator. When the laser locator detects the position of the glass slide rack, the second motor is started by the controller to drive the second screw to rotate to move the connecting plate to the left, and then the connecting plate will drive the card plate to pass through the slot and make The left end of the card plate is stuck in the card slot. At this time, the second motor is started to drive the second screw to rotate in the opposite direction, so that the connecting plate moves to the right. Then the connecting plate hooks the card slot through the card plate to pull the glass slide rack out of the partition. When it moves to the appropriate position, the second motor is turned off to fix the position of the connecting plate. Then the glass slide on the glass slide rack can be observed through a microscope to perform quantitative analysis on its dental pulp stem cells. When the laser locator detects that there is no glass slide rack in the corresponding position, the first motor will be controlled by the controller to drive the first screw to rotate so that the slider drives the placement box to rise. When the laser locator re-detects the position of the glass slide rack, the controller will be controlled to turn off the first motor to fix the position of the placement box, which is convenient for the feeding component to load materials for quantitative analysis.

[0027] The utility model provides a dental pulp stem cell quantitative analysis device. Compared with the prior art, it has the following beneficial effects:

[0028] The dental pulp stem cell quantitative analysis device can conveniently fix sizes of different specifications by means of the provided limit frames and connecting rods. When in use, the pressing plate is pressed to push the top plate to move via the connecting rod, and then the top plate drives the limit frames to move to expand the distance between the limit frames. Then, the slide rack for storing the slides is placed on the partition so that it is located between the limit frames. At this time, the pressing plate is released and the elastic force of the fixing box drives the limit frames so that the slide rack is located in the middle of the partition to position it. The slide racks of different specifications can be adapted to be positioned for convenience and speed.

[0029] The dental pulp stem cell quantitative analysis device facilitates pulling the glass slide rack to load materials for analysis through a microscope by means of the provided connecting plate and the clamping plate. When in use, the second motor is started to drive the second screw to rotate so that the connecting plate moves to the left, and then the connecting plate drives the clamping plate to pass through the inside of the slot and clamp the bottom surface of the clamping plate in the clamping slot. At this time, the second motor is started again to drive the second screw to rotate in the opposite direction so that the connecting plate moves to the right, and then the connecting plate hooks the clamping slot through the clamping plate to pull the glass slide rack out of the partition. When it moves to a suitable position, the second motor is turned off to fix the position of the connecting plate, and then the glass slide on the glass slide rack can be observed through a microscope to perform a quantitative analysis of the dental pulp stem cells. The device is convenient, fast and practical. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] Figure 1 It is a three-dimensional schematic diagram of the utility model;

[0031] Figure 2 It is a three-dimensional schematic diagram of the detection mechanism of the utility model;

[0032] Figure 3 It is a front cross-sectional perspective schematic diagram of the detection mechanism of the utility model;

[0033] Figure 4 For the utility model Figure 3 The enlarged schematic diagram at A in the middle;

[0034] Figure 5 It is a side cross-sectional stereoscopic schematic diagram of the detection mechanism of the utility model.

[0035] In the figure: 1. base; 2. detection mechanism; 21. placement component; 211. fixed box; 212. partition; 213. placement box; 214. placement frame; 215. limit groove; 216. limit frame; 217. top plate; 218. connecting rod; 219. pressure plate; 2110. movable groove; 2111. spring; 22. lifting component; 221. first motor; 222. slide groove; 223. first screw rod; 224. slider; 225. laser locator; 23. feeding component; 231. notch; 232. card slot; 233. connecting plate; 234. fixed frame; 235. connecting groove; 236. second screw rod; 237. second motor; 238. mounting groove; 239. card plate; 2310. slide rack; 3. microscope; 4. controller. DETAILED DESCRIPTION

[0036] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments of the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0037] Embodiment: A dental pulp stem cell quantitative analysis device comprises a base 1, on the top surface of which a microscope 3, a controller 4 and a detection mechanism 2 are fixedly mounted.

[0038] The detection mechanism 2 includes a placement component 21, a lifting component 22 and a feeding component 23;

[0039] The placement assembly 21 includes a fixed box 211 fixedly arranged on the left side of the base 1, a placement box 213 is movably arranged in the fixed box 211, a placement rack 214 is movably arranged in the placement box 213, a plurality of partitions 212 are movably arranged in the placement rack 214, a glass slide rack 2310 is placed on the partition 212, a movable groove 2110 is arranged at the inner bottom of the placement rack 214, four limit racks 216 are movably arranged in the movable groove 2110, and four limit racks 215 are arranged on the placement rack 214 and the partition 212, and the limit racks 216 are correspondingly penetrated in the limit racks 215 A spring 2111 is fixedly connected between the lower end of the limit frame 216 and the movable groove 2110, and a connecting assembly is movably installed on the top surface of the placement frame 214, and the connecting assembly includes a top plate 217, a connecting rod 218 and a pressing plate 219; two top plates 217 are arranged above the placement frame 214, and the two top plates 217 are arranged in parallel at the top of the limit frame 216, and the two top plates 217 are perpendicular to the setting direction of the spring 2111; a connecting rod 218 is movably hinged in the middle of each top plate 217; and a pressing plate 219 is movably hinged at the top of the two connecting rods 218;

[0040] The lifting assembly 22 is arranged inside the fixed box 211, and the lifting assembly 22 is respectively connected to the placement box 213 and the controller 4, and is used to adjust the height of the placement box 213. The lifting assembly 22 includes a slide 222, a first screw 223, a first motor 221, a slider 224 and a laser locator 225; the slide 222 is opened inside the rear side wall of the fixed box 211; the first screw 223 is rotatably arranged in the slide 222; the first motor 221 is fixedly arranged at the top of the slide 222, the first motor 221 is connected to the controller 4, and the output shaft of the first motor 221 is coaxially fixedly connected with the upper end of the first screw 223; the slider 224 is fixedly arranged at the rear side of the placement box 213, and the slider 224 is slidably embedded in the slide 222, and the first screw 223 penetrates and threadedly engages with the slider 224; the laser locator 225 is fixedly arranged on the right side wall of the inner cavity of the fixed box 211, and is connected to the controller 4;

[0041] The feeding assembly 23 is arranged on the right side of the fixed box 211, the feeding assembly 23 is connected to the controller 4, and the feeding assembly 23 is communicated with the inside of the fixed box 211, and is used to drive the glass slide in the fixed box 211 to move to the bottom of the microscope 3, and quantitatively analyze the dental pulp stem cells on the glass slide. The feeding assembly 23 includes a notch 231, a fixed frame 234, a second screw 236 and a clamping plate 239; the notch 231 is opened on the right side of the fixed box 211; the fixed frame 234 is fixedly arranged on the right side of the fixed box 211, and a connecting plate 233 is movably arranged in the fixed frame 234, and a mounting plate 239 is opened on the bottom surface of the fixed frame 234. Slot 238, a connecting slot 235 is provided on the front side of the fixed frame 234; a second screw rod 236 is movably arranged inside the connecting slot 235, a second motor 237 is fixedly arranged on the right side of the fixed frame 234, the second motor 237 is connected to the controller 4, the output shaft of the second motor 237 is coaxially fixedly connected with the second screw rod 236, the second screw rod 236 passes through and threadedly engages with one end of the connecting plate 233; a clamping plate 239 is fixedly arranged on the left side of the connecting plate 233, a clamping slot 232 is provided on the right side of the upper surface of the glass slide rack 2310, the clamping plate 239 is used to movably pass through the slot 231 and be clamped in the clamping slot 232.

[0042] The working principle of the above embodiment is as follows: when in use, the user places the glass slide on the glass slide rack 2310, then presses the pressure plate 219 to push the top plate 217 to move through the connecting rod 218, and then the top plate 217 will drive the limit frame 216 to move to expand the distance between the limit frames 216, and then place the glass slide rack 2310 on the partition 212 so that it is located between the limit frames 216, then release the pressure plate 219, and the limit frame 216 is driven to reset by the elastic force of the spring 2111, so that the glass slide rack 2310 is located in the middle of the partition 212 and fixed in position, and then the placement rack 214 is picked up and placed inside the placement box 213, at this time, the position of the glass slide rack 2310 can be detected by the laser locator 225, when the laser locator 225 detects the position of the glass slide rack 2310, the second motor 237 is started by the controller 4 to drive the second screw 236 to rotate to make the connecting plate 233 move to the left, and then the connecting plate 233 will drive the card plate 239 to pass through the slot 2 31, and the left end of the card plate 239 is stuck in the card slot 232, then the second motor 237 is started to drive the second screw 236 to rotate in the opposite direction, so that the connecting plate 233 moves to the right, and then the connecting plate 233 hooks the card slot 232 through the card plate 239 to pull the glass slide rack 2310 out of the partition 212. When it moves to a suitable position, the second motor 237 is turned off to fix the position of the connecting plate 233, and then the glass slide on the glass slide rack 2310 can be observed through the microscope 3 to perform quantitative analysis on the dental pulp stem cells. When the laser locator 225 detects that there is no glass slide rack 2310 at the corresponding position, the first motor 221 is controlled by the controller 4 to drive the first screw 223 to rotate so that the slider 224 drives the placement box 213 to rise. When the laser locator 225 detects the position of the glass slide rack 2310 again, the controller 4 is controlled to turn off the first motor 221 to fix the position of the placement box 213, so as to facilitate the feeding component 23 to load the material for quantitative analysis.

[0043] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include"-"comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process-method-article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process-method-article or device.

[0044] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A dental pulp stem cell quantitative analysis device, comprising a base (1), a microscope (3) and a controller (4) being fixedly mounted on the top surface of the base (1), characterized in that: The top surface of the base (1) is also provided with a detection mechanism (2), and the detection mechanism (2) comprises: A placement assembly (21) comprises a fixed box (211) fixedly arranged on the left side of the base (1), a placement box (213) being movably arranged in the fixed box (211), a placement rack (214) being movably arranged in the placement box (213), a plurality of partitions (212) being movably arranged in the placement rack (214), and a glass slide rack (2310) being placed on the partition (212); A lifting component (22) is arranged inside the fixed box (211), and the lifting component (22) is connected to the placement box (213) and the controller (4) respectively, and is used to adjust the height of the placement box (213); A feeding assembly (23) is arranged on the right side of the fixed box (211), the feeding assembly (23) is connected to the controller (4), and the feeding assembly (23) is communicated with the interior of the fixed box (211), and is used to drive the glass slide in the fixed box (211) to move to the bottom of the microscope (3) to quantitatively analyze the dental pulp stem cells on the glass slide; The inner bottom of the placement rack (214) is provided with a movable groove (2110), and four limit racks (216) are movably provided in the movable groove (2110). The placement rack (214) and the partition (212) are both provided with four limit racks (215), and the limit racks (216) are correspondingly inserted into the limit racks (215). A spring (2111) is fixedly connected between the lower end of the limit rack (216) and the movable groove (2110), and a connecting component is movably installed on the top surface of the placement rack (214); The connection component comprises: Two top plates (217), wherein the two top plates (217) are disposed above the placement frame (214), the two top plates (217) are disposed in parallel at the top of the limiting frame (216), and the two top plates (217) are disposed perpendicularly to the direction in which the spring (2111) is disposed; A connecting rod (218), wherein a connecting rod (218) is movably hinged at the middle of each top plate (217); A pressure plate (219) is movably hingedly connected to the top ends of the two connecting rods (218).

2. A dental pulp stem cell quantitative analysis device according to claim 1, characterized in that: The lifting assembly (22) comprises: A slide groove (222) is provided inside the rear side wall of the fixed box (211); A first screw rod (223) rotatably disposed in the slide groove (222); A first motor (221) is fixedly arranged at the top of the slide slot (222), the first motor (221) is connected to the controller (4), and the output shaft of the first motor (221) is coaxially fixedly connected to the upper end of the first screw rod (223); A slider (224) is fixedly arranged on the rear side of the placement box (213), and the slider (224) is slidably embedded in the slide groove (222), and the first screw rod (223) penetrates and is threadedly engaged with the slider (224); The laser locator (225) is fixedly disposed on the right side wall of the inner cavity of the fixed box (211) and is connected to the controller (4).

3. The dental pulp stem cell quantitative analysis device according to claim 1, characterized in that: The feeding assembly (23) comprises: A notch (231) is provided on the right side of the fixing box (211); A fixed frame (234) is fixedly arranged on the right side of the fixed box (211), a connecting plate (233) is movably arranged in the fixed frame (234), a mounting groove (238) is provided on the bottom surface of the fixed frame (234), and a connecting groove (235) is provided on the front side of the fixed frame (234); a second screw rod (236) movably arranged inside the connecting groove (235); a second motor (237) is fixedly arranged on the right side of the fixing frame (234); the second motor (237) is connected to the controller (4); an output shaft of the second motor (237) is coaxially fixedly connected to the second screw rod (236); the second screw rod (236) penetrates through and is threadedly engaged with one end of the connecting plate (233); A clamping plate (239) is fixedly arranged on the left side of the connecting plate (233); a clamping slot (232) is provided on the right side of the upper surface of the glass slide rack (2310); and the clamping plate (239) is used to movably penetrate the notch (231) and be clamped in the clamping slot (232).

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