Detection device for enzyme-linked immunosorbent assay
By using electric push rods, pulleys and drive motor systems in the microplate reader to fix the microplate, the problem of pouring or shaking of the microplate during the microplate reader operation is solved, the stability of antibodies and antigens is achieved, and the detection is improved. accuracy.
Patent Information
- Application Number
- CN202420844868.9
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-04-23
- Publication Date
- 2025-01-14
- Estimated Expiration
- 2034-04-23
AI Technical Summary
During use of existing microplate reader, the microplate is prone to tipping or shaking, resulting in the loss of antibodies or antigens, especially when the lid needs to be lifted up or the use of pull-out structures.
An enzyme-linked immunosorbent assay detection device was designed, using an electric push rod and pulley system to fix the microplate, and the screw slider is driven to move in the linear guide rail through the driving motor, and the microplate is clamped with the fixed and movable card plates. , ensure its stability.
Effectively prevent the microplate from pouring or shaking during operation, ensuring the stability of antigens and antibodies, thereby improving the accuracy and reliability of detection.
Smart Images
Figure CN222353917U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of tumor index detection, in particular to an enzyme-linked immunosorbent assay detection device. Background Art
[0002] Enzyme-linked immunosorbent assay (ELISA): It is the most widely used technology in enzyme immunoassay technology. Its basic method is to adsorb known antigens or antibodies on the surface of a solid phase carrier (polystyrene microplate), allow the enzyme-labeled antigen-antibody reaction to proceed on the solid phase surface, and use a washing method to remove free components in the liquid phase. Commonly used ELISA methods include the double antibody sandwich method and the indirect method. The former is used to detect macromolecular antigens, and the latter is used to determine specific antibodies.
[0003] In the prior art, the enzyme-linked immunosorbent assay requires the use of an enzyme reader, which can effectively detect tumor markers. However, during use, the existing enzyme reader requires the microplate and the antibodies or antigens inside it to be placed inside the enzyme reader. Some models of enzyme readers require the cover to be lifted and then the microplate is placed therein. This method is prone to tipping over the microplate during operation, thereby causing the antibodies and antigens to tip over. During use, the enzyme reader that uses a pull-out structure to place the microplate has a poor control of the pull-out speed, which can easily cause the microplate to shake, thereby causing the antigen or antibody to be lost. Therefore, we propose an enzyme-linked immunosorbent assay detection device. Utility Model Content
[0004] The purpose of the utility model is to solve the shortcomings existing in the prior art. The enzyme-linked immunosorbent assay requires the use of an enzyme reader, and the enzyme reader can effectively detect tumor indicators. However, during the use of the existing enzyme reader, a microplate and the antibodies or antigens therein need to be placed inside the enzyme reader. Among them, some types of enzyme readers require the cover to be lifted up and then the microplate is placed therein. In this way, the microplate is easily tipped over during operation, thereby causing the antibody and antigen to tip over. During the use of the enzyme reader that uses a pull-out structure to place the microplate, its pull-out speed is not well controlled, and it is also easy to cause the microplate to shake, thereby causing the antigen or antibody to be lost.
[0005] In order to achieve the above purpose, the utility model adopts the following technical solutions:
[0006] An enzyme-linked immunosorbent assay detection device includes an enzyme-labeled instrument body, a base is installed at the bottom of the enzyme-labeled instrument body, a display screen is installed at the top of the enzyme-labeled instrument body, and a detection chamber is opened on the right side of the enzyme-labeled instrument body, and a container is slidably installed inside the detection chamber, and electric push rods are installed on both sides of the container and inside the detection chamber, and several groups of pulleys are symmetrically installed on both sides of the bottom of the container, and a accommodating cavity is opened through the top of the container, and a fixed card plate and a movable card plate are installed inside the accommodating cavity.
[0007] As a preferred solution of the utility model, a sealing strip is bonded to the outer periphery of the container and the inner wall of the detection chamber, and a manual handle is also installed on the front of the container, and the sealing strip is made of silicone material.
[0008] As a preferred solution of the utility model, a connection pad is installed at the fixed end of the electric push rod and located inside the detection chamber. The fixed end of the electric push rod is fixedly connected to the detection chamber through the connection pad, and the output end of the electric push rod is fixedly connected to the container.
[0009] As a preferred solution of the utility model, a first support plate is installed on the right side of the fixed card plate and inside the accommodating cavity, and an anti-slip pad is also bonded to the top of the fixed card plate, and the material used for the anti-slip pad is silicone material.
[0010] As a preferred solution of the utility model, a second support plate is installed on the left side of the movable clamping plate and located inside the accommodating cavity, and a lead screw slider is installed on the back side of the movable clamping plate, and a linear guide rail is opened on the periphery of the lead screw slider and located on the inner back side of the accommodating cavity, and a lead screw is rotatably installed inside the linear guide rail, and a drive motor is installed on the right side of the lead screw and located inside the container, and the lead screw slider is slidably connected to the linear guide rail and intersects with the lead screw thread.
[0011] As a preferred solution of the utility model, a micro-porous plate is clamped on the top of the fixed clamping plate and the movable clamping plate.
[0012] As a preferred solution of the utility model, the pulley is rotatably connected to the container and slidably connected to the detection chamber.
[0013] The technical effect of adopting the above further solution is that the microplate can be effectively fixed by the fixed clamping plate and the movable clamping plate, thereby effectively ensuring the stability of the microplate.
[0014] Compared with the prior art, the beneficial effects of the utility model are:
[0015] In the utility model, through the setting of the electric push rod, the electric push rod can effectively push the container so that it can slide stably in the detection chamber, and the pulley can effectively make the container more stable during the sliding process, avoiding the antigen or antibody on the internal microplate from tipping over due to shaking, and the driving motor can effectively drive the screw rod to rotate, thereby driving the screw rod slider to move in the linear guide rail, thereby moving the movable clamping plate, and cooperating with the fixed clamping plate to clamp the microplate, so that the microplate can effectively remain stable when moving. BRIEF DESCRIPTION OF THE DRAWINGS
[0016] Figure 1 It is a schematic diagram of the overall structure of the utility model;
[0017] Figure 2 This is a schematic diagram of the container storage structure of the utility model;
[0018] Figure 3 This is a schematic diagram of the structure of the electric push rod of the utility model;
[0019] Figure 4 This is a schematic diagram of the structure of the connection pad of the utility model.
[0020] Legend: 1. ELISA instrument body; 2. Base; 3. Display screen; 4. Detection chamber; 5. Container; 51. Sealing strip; 52. Manual handle; 6. Electric push rod; 61. Connecting pad; 7. Pulley; 8. Accommodating chamber; 9. Fixed card plate; 91. First support plate; 92. Anti-slip pad; 10. Movable card plate; 101. Second support plate; 102. Screw slider; 103. Linear guide; 104. Screw; 105. Drive motor; 11. Microplate. DETAILED DESCRIPTION
[0021] The technical solutions in the embodiments of the utility model will be clearly and completely described below in conjunction with the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, rather than all of the embodiments. Based on the embodiments of the utility model, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the utility model.
[0022] In order to facilitate the understanding of the present invention, the present invention will be described more comprehensively below with reference to the relevant literature, and several embodiments of the present invention are given. However, the present invention can be implemented in many different forms and is not limited to the embodiments described herein. On the contrary, the purpose of providing these embodiments is to make the disclosure of the present invention more thorough and comprehensive.
[0023] It should be noted that when an element is referred to as being "fixed on" another element, it may be directly on the other element or there may also be a central element. When an element is considered to be "connected to" another element, it may be directly connected to the other element or there may be a central element at the same time. The terms "vertical", "horizontal", "left", "right" and similar expressions used in this article are for illustrative purposes only.
[0024] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by technicians in the technical field of the present invention. The terms used in the specification of the present invention are only for the purpose of describing specific embodiments and are not intended to limit the present invention. The term "and / or" used in this article includes any and all combinations of one or more related listed items.
[0025] Example 1
[0026] like Figure 1-4 As shown, the utility model provides a technical solution: an enzyme-linked immunosorbent assay detection device, comprising an ELISA instrument body 1, a base 2 is installed at the bottom of the ELISA instrument body 1, and a display screen 3 is installed on the top of the ELISA instrument body 1, and the detection value can be effectively displayed through the display screen 3, and a detection chamber 4 is opened on the right side of the ELISA instrument body 1, and a container 5 is slidably installed inside the detection chamber 4, and an electric push rod 6 is installed on both sides of the container 5 and inside the detection chamber 4, and a plurality of groups of pulleys 7 are symmetrically installed on both sides of the bottom of the container 5, and a accommodating cavity 8 is opened through the top of the container 5, and a fixed card plate 9 and a movable card plate 10 are installed inside the accommodating cavity 8.
[0027] Example 2
[0028] like Figure 1-4 As shown, a sealing strip 51 is bonded to the periphery of the container 5 and the inner wall of the detection chamber 4, and a manual handle 52 is installed on the front of the container 5. The sealing strip 51 is made of silicone material, and the manual handle 52 is convenient for use in special circumstances.
[0029] A connecting pad 61 is installed at the fixed end of the electric push rod 6 and located inside the detection chamber 4. The fixed end of the electric push rod 6 is fixedly connected to the detection chamber 4 through the connecting pad 61, and the output end of the electric push rod 6 is fixedly connected to the container 5. The electric push rod 6 can stably push the container 5 out to improve stability.
[0030] A first support plate 91 is installed on the right side of the fixed card plate 9 and inside the accommodating cavity 8, and an anti-skid pad 92 is bonded to the top of the fixed card plate 9. The anti-skid pad 92 is made of silicone material, and the stability of the microporous plate 11 can be maintained by the anti-skid pad 92.
[0031] A second support plate 101 is installed on the left side of the movable card plate 10 and located inside the accommodating chamber 8, and a screw slider 102 is installed on the back side of the movable card plate 10. A linear guide rail 103 is provided on the periphery of the screw slider 102 and located on the inner back side of the accommodating chamber 8, and a screw rod 104 is rotatably installed inside the linear guide rail 103, and a driving motor 105 is installed on the right side of the screw rod 104 and located inside the container 5. The screw slider 102 is slidably connected to the linear guide rail 103 and intersects with the thread of the screw rod 104. The driving motor 105 can effectively drive the screw rod 104, thereby driving the screw slider 102 to move linearly, so that the movable card plate 10 clamps the microplate 11.
[0032] A micro-porous plate 11 is clamped on the top of the fixed clamping plate 9 and the movable clamping plate 10 .
[0033] The pulley 7 is rotationally connected to the container 5 and slidably connected to the detection chamber 4, which can make the movement of the container 5 more stable.
[0034] The working process of the utility model is as follows: when using an enzyme-linked immunosorbent assay detection device to detect tumor indicators, first, the microplate 11 is taken out, and the tumor sample and the solution are placed in the microplate 11. Then, the container 5 is pushed out at a uniform speed in the detection chamber 4 by the electric push rod 6. At the same time, the pulley 7 at the bottom of the container 5 also keeps the container 5 stable. When the container 5 is completely pushed out, at this time, one end of the microplate 11 is stably placed on the fixed card plate 9 in the accommodating chamber 8 so that it is fully in contact with the anti-slip pad 92. After completion, the electric push rod 6 is driven to push the container 5 out of the detection chamber 4. The lead screw 105 drives the lead screw slider 102 to slide inside the linear guide 103 toward the position of the microplate 11, supports the other end of the microplate 11, and relies on the second support plate 101 to clamp the microplate 11. After completion, the container 5 can be recovered to the detection chamber 4 through the electric push rod 6 and the pulley 7, and the ELISA instrument body 1 is controlled by the display screen 3 to detect the tumor sample. The utility model can effectively ensure the stability of the microplate and the antigens and antibodies therein through the design, thereby avoiding affecting the detection of the enzyme-linked immunosorbent assay.
[0035] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.
Claims
1. An enzyme-linked immunosorbent assay detection device, comprising an ELISA body (1), characterized in that: A base (2) is installed at the bottom of the microplate reader body (1), a display screen (3) is installed at the top of the microplate reader body (1), and a detection chamber (4) is opened on the right side of the microplate reader body (1), and a container (5) is slidably installed inside the detection chamber (4), and electric push rods (6) are installed on both sides of the container (5) and inside the detection chamber (4), and a plurality of groups of pulleys (7) are symmetrically installed on both sides of the bottom of the container (5), and a receiving chamber (8) is opened through the top of the container (5), and a fixed card plate (9) and a movable card plate (10) are installed inside the receiving chamber (8).
2. An ELISA detection device according to claim 1, characterized in that: A sealing strip (51) is bonded to the outer periphery of the container (5) and the inner side wall of the detection chamber (4), and a manual handle (52) is also installed on the front of the container (5). The sealing strip (51) is made of silicone material.
3. An ELISA detection device according to claim 1, characterized in that: A connection pad (61) is installed at the fixed end of the electric push rod (6) and is located inside the detection chamber (4); the fixed end of the electric push rod (6) is fixedly connected to the detection chamber (4) via the connection pad (61), while the output end of the electric push rod (6) is fixedly connected to the container (5).
4. The ELISA detection device according to claim 1, characterized in that: A first support plate (91) is installed on the right side of the fixed card plate (9) and inside the accommodating cavity (8), and an anti-skid pad (92) is also bonded to the top of the fixed card plate (9), wherein the anti-skid pad (92) is made of silicone material.
5. The ELISA detection device according to claim 1, characterized in that: A second support plate (101) is installed on the left side of the movable clamping plate (10) and located inside the accommodating chamber (8), and a lead screw slider (102) is installed on the back side of the movable clamping plate (10), and a linear guide rail (103) is provided on the periphery of the lead screw slider (102) and located on the inner back side of the accommodating chamber (8), and a lead screw (104) is rotatably installed inside the linear guide rail (103), and a driving motor (105) is installed on the right side of the lead screw (104) and located inside the container (5), and the lead screw slider (102) is slidably connected to the linear guide rail (103) and intersects with the thread of the lead screw (104).
6. An ELISA detection device according to claim 1, characterized in that: A microporous plate (11) is clamped on the top of the fixed clamping plate (9) and the movable clamping plate (10).
7. An ELISA detection device according to claim 1, characterized in that: The pulley (7) is rotationally connected to the container (5) and slidably connected to the detection chamber (4).