Portable nucleic acid molecule diagnosis device

Abstract

The utility model belongs to the field of nucleic acid detection, and particularly relates to a portable nucleic acid molecule diagnosis device which comprises an upper shell and a lower cover, the upper shell is fixedly connected with the lower cover, a sample adding hole is formed in the upper shell, a state display is arranged on the upper shell, a button is arranged at the top of the upper shell, and a battery is arranged on the button. The upper shell comprises a detachable part and a fixed base, the detachable part and the fixed base are detachable, the detachable part is provided with a connecting part, the connecting part is provided with an electronic contact point, the middle part of one side of the connecting part is provided with an in-place detection sensor, one side of the fixed base is provided with a connecting port, and the connecting port is connected with the electronic contact point. The liquid separation reaction assembly is arranged below the detachable part; the device is simple in structure, the size of the device can be made into the size of a palm, the device is convenient to carry and move, meanwhile, sample diversion, heating and chromogenic reaction can be completed through the built-in liquid separation reaction assembly, and a result can be read quickly.

Description

Technical Field

[0001] The utility model relates to the field of nucleic acid detection, in particular to a portable nucleic acid molecule diagnostic device. Background Art

[0002] Nucleic acid molecular diagnostic methods are widely used. Molecular diagnostic methods usually involve rapid amplification of nucleic acid molecules by the RNA or DNA components in the sample under the joint action of various specific enzyme components. Molecular diagnostic methods have the characteristics of strong specificity, high sensitivity, simple operation, and time saving. For example, reverse transcription-polymerase chain reaction, real-time fluorescence quantitative method, etc. Among them, reverse transcription-polymerase chain reaction is to amplify viral nucleic acid first, and then analyze the amplified product by gel electrophoresis. This method can only be used for qualitative analysis, and the lid needs to be opened during the experiment. The operation is complicated and the product is easily contaminated to produce false positive results; real-time fluorescence quantitative method uses probes or dyes to quantitatively analyze viral RNA, and has strong specificity and high sensitivity. It can effectively avoid contamination caused by human operation and reduce the probability of false positive results. However, it requires expensive experimental equipment and experienced experimental personnel, which limits the wide application of this method.

[0003] In actual use by personnel in this field, firstly, various types of luminescence or color change reaction detection operations are difficult, the equipment is large in size, and requires a high detection threshold; secondly, there is a lack of integrated equipment with optimized size, complete functions and easy operation. If the current equipment is used, the operation process is relatively complicated, the test results cannot be obtained intuitively, and it cannot be applied to instant sample testing in various occasions. Utility Model Content

[0004] In order to make up for the shortcomings of the prior art and solve the problems of difficult detection operation and high detection threshold, the utility model proposes a portable nucleic acid molecule diagnostic device.

[0005] The technical solution adopted by the utility model to solve its technical problems is: a portable nucleic acid molecular diagnostic device described in the utility model includes a box body, a liquid separation reaction component and a main control circuit component, the box body includes an upper shell and a lower cover, the upper shell is fixedly connected to the lower cover, a sample addition hole is arranged on the upper shell, a status display is arranged on the upper shell, a button is arranged on the top of the upper shell, the upper shell includes a detachable part and a fixed base, the detachable part and the fixed base are detachable, a connecting part is arranged on the detachable part, an electronic contact point is arranged on the connecting part, an in-place detection sensor is arranged in the middle of one side of the connecting part, a connecting port is arranged on one side of the fixed base, and the liquid separation reaction component is arranged below the detachable part.

[0006] Preferably, the liquid separation reaction component includes a liquid conducting groove, a liquid separation structure, a sealing gasket, a color reaction layer, a temperature adjustment unit and a base, the liquid separation structure is arranged at the bottom of the liquid conducting groove, the color reaction layer is arranged at the bottom of the liquid separation structure, the sealing gasket is arranged between the color reaction layer and the liquid separation structure, the temperature adjustment unit is arranged at the bottom of the color reaction layer, the base is arranged at the bottom of the temperature adjustment unit, and a plurality of reaction chambers are opened on the top of the color reaction layer.

[0007] Preferably, the main control circuit assembly includes a main control component, a temperature control component, a detection light control component, a color sensor, a display component and a signal transmission component. The main control component is electrically connected to the temperature control component, the detection light control component is electrically connected to the main control component, the color sensor is electrically connected to the main control component, the display component is electrically connected to the main control component, and the signal transmission component is electrically connected to the main control component.

[0008] Preferably, the sealing gasket is made of silicone rubber.

[0009] Preferably, the status display and buttons can be combined into a display device.

[0010] Preferably, the in-position detection sensor is a Hall sensor or a proximity sensor.

[0011] The utility model is beneficial in that:

[0012] 1. The utility model has a simple structure and can be made into a palm-sized volume, which is easy to carry and move. It is suitable for instant sample detection in various occasions. At the same time, the sample diversion, heating and color development reaction can be completed through the built-in liquid separation reaction component, and the results can be read quickly;

[0013] 2. The utility model can be used by non-professionals, such as physical examination institutions or family members, thus expanding the scope of use of nucleic acid detection devices.

[0014] 3. The utility model does not require the purchase of additional professional equipment during use. The device contains all the reaction consumables required for nucleic acid testing, reducing the cost of use. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] In order to more clearly illustrate the embodiments of the utility model or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the utility model. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0016] Figure 1 It is a schematic diagram of the main body of the utility model;

[0017] Figure 2 This is an overall schematic diagram of the upper shell of the utility model;

[0018] Figure 3 It is a schematic diagram of an upper housing of another embodiment of the utility model;

[0019] Figure 4 This is a schematic diagram of the assembly of the upper housing of the utility model;

[0020] Figure 5 This is a schematic diagram of the exploded structure of the liquid separation reaction component of the utility model;

[0021] Figure 6 It is a structural schematic diagram of the control circuit component of the utility model.

[0022] In the figure: 100, box body; 101, upper shell; 1011, sample injection hole; 1012, status display; 1013, button; 1014, display device; 1015, detachable part; 10151, connection part; 10152, electronic contact point; 10153, in-place detection sensor; 1016, fixed base; 10161, connection port; 102, lower cover; 200, liquid separation reaction component; 201, liquid guide groove; 202, liquid separation structure; 203, sealing gasket; 204, color reaction layer; 205, temperature adjustment unit; 206, base; 300, main control circuit component; 301, main control unit; 302, temperature control unit; 303, detection light control unit; 304, color sensor; 305, display unit; 306, signal transmission unit. DETAILED DESCRIPTION

[0023] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0024] See also Figure 1-Figure 6As shown, a portable nucleic acid molecular diagnostic device includes a box body 100, a liquid separation reaction component 200 and a main control circuit component 300. The box body 100 includes an upper shell 101 and a lower cover 102. The upper shell 101 is fixedly connected to the lower cover 102. The upper shell 101 is provided with a sample addition hole 1011, a status display 1012, and a button 1013 on the top of the upper shell 101. The upper shell 101 includes a detachable part 1015 and a fixed part 1016. The fixed base 1016, the detachable part 1015 and the fixed base 1016 are detachable, the detachable part 1015 is provided with a connecting part 10151, the connecting part 10151 is provided with an electronic contact point 10152, a position detection sensor 10153 is provided in the middle of one side of the connecting part 10151, a connecting port 10161 is provided on one side of the fixed base 1016, and the liquid separation reaction component 200 is provided below the detachable part 1015;

[0025] When working, the connecting part 10151 and the connecting port 10161 are complementary structures, and the connection between the two parts can be completed by some snap-fit ​​structures or screw locking mechanisms; wherein, the connecting part 10151 is an asymmetric shape to prevent misalignment of the connection, and this structure is in an asymmetric position in the overall structure, and one or more can be set to improve the connection strength; wherein, the electronic contact points 10152 arranged on both sides of the connecting part 10151 are used to conduct the circuit between the fixed base 1016 and the detachable part 1015. Since the electronic contact points 10152 are arranged at the outer edge of the connecting part 10151, the electronic contact points 10152 can be connected to perform functions such as installation in place judgment and information transmission; at the same time, an in-place detection sensor 10153 for in-place detection is also provided.

[0026] Further, the liquid separation reaction component 200 includes a liquid guide groove 201, a liquid separation structure 202, a sealing gasket 203, a color reaction layer 204, a temperature adjustment unit 205 and a base 206, the liquid separation structure 202 is arranged at the bottom of the liquid guide groove 201, the color reaction layer 204 is arranged at the bottom of the liquid separation structure 202, the sealing gasket 203 is arranged between the color reaction layer 204 and the liquid separation structure 202, the temperature adjustment unit 205 is arranged at the bottom of the color reaction layer 204, the base 206 is arranged at the bottom of the temperature adjustment unit 205, and a plurality of reaction chambers are opened on the top of the color reaction layer 204;

[0027] When working, the liquid separation reaction assembly 200 includes six main parts, which are connected in sequence, namely, a liquid guide groove 201, a liquid separation structure 202, a sealing gasket 203, a color reaction layer 204, a temperature adjustment unit 205, and a base 206. Accordingly, the functional structures of the liquid separation and reaction module 200 are in a vertically stacked state, and can be matched with other structures by setting a bayonet structure on each functional structure. Of course, the functional modules can also be bonded together by adhesives, or fasteners such as screws can be used to lock all parts. The technicians can adopt appropriate methods according to different design requirements, which will not be described one by one here.

[0028] Further, the main control circuit assembly 300 includes a main control 301, a temperature control component 302, a detection light control component 303, a color sensor 304, a display component 305 and a signal transmission component 306, the main control 301 is electrically connected to the temperature control component 302, the detection light control component 303 is electrically connected to the main control 301, the color sensor 304 is electrically connected to the main control 301, the display component 305 is electrically connected to the main control 301, and the signal transmission component 306 is electrically connected to the main control 301;

[0029] When working, the main control 301 is used to complete the functions of temperature management, signal processing, result interpretation, information display, and information transmission of the equipment, and is also used to complete the signal processing and function realization of all modules. There is at least one temperature control unit 302, and its temperature can be controlled individually or separately by the main control unit 301; there can also be multiple detection light control components 303 and color sensor sensors 304, and the main control unit 301 can control one or more detection light control components 303 and color sensor sensors 304 individually; one detection light control component 303 can control one detection light, and can also include multiple detection lights; the color sensor sensor 304 can be composed of one or more color sensors and a control module to obtain the color change values ​​of one or more reaction chambers, and the different detection lights in the detection light control component 303 have multiple wavelengths.

[0030] Further, the sealing gasket 203 is made of silicone rubber;

[0031] When working, the elastic material has the characteristics of being hydrophobic, non-adsorbing nucleic acids and being temperature-resistant, and can also be other elastic materials.

[0032] Further, the status display 1012 and the button 1013 may be combined into a display device 1014;

[0033] When working, this is another embodiment of the present device, and the display device 1014 combines the functions of the status display 1012 and the button 1013.

[0034] Further, the in-place detection sensor 10153 is a Hall sensor or a proximity sensor;

[0035] A general term for sensors that replace contact detection methods such as limit switches and are designed to detect objects without contact. They can convert the movement and presence information of the detected object into electrical signals. Among the detection methods that convert to electrical signals, there are methods that utilize eddy currents generated in the metal body of the detected object due to electromagnetic induction, methods that capture the capacitance change of electrical signals caused by the approach of the detected object, and methods such as sharp stones and guide switches.

[0036] Working principle: When using the device, it is necessary to add the external test sample into the device through the sample addition hole 1011, and press the button 1013 to start the test. The test sample enters the liquid separation reaction component 200, wherein the liquid guide groove 201 is used to guide the sample added through the sample addition hole 1011 to enter, so that the liquid is evenly guided into the liquid circulation pipeline of the liquid separation structure 202, and then the upper liquid separation structure 202 evenly distributes the sample into a plurality of different fluid channels, and the lower liquid separation structure 202 introduces the liquid into the lower liquid separation structure 202 through the liquid outlet, and the color sensor 304 performs sensing measurement, and the sealing gasket 203 seals the liquid separation structure 202 and the color reaction layer 204, and the temperature adjustment unit 205 provides the color reaction layer 204 with a specified reaction temperature. When the test is in progress, the light source of the incident light is lit in sequence according to a certain time sequence, and the readings are respectively made according to the time sequence. The optical signals of each reaction chamber are electrically connected to the detection lamp array independently through the detection lamp control component 303 after the color reaction layer 204 is heated to the specified temperature and the sample in the reaction chamber changes color. The light path of the light after lighting irradiates through the reaction chamber and finally reaches the color sensor 304. The color sensor 304 transmits the optical signal to the main control 301. The main control 301 displays the positive or negative test result according to the optical signal instruction indicator array, which is convenient for the operator to observe and judge. The result can be observed after 30 minutes. The color development method of this device is the hydroxyl tea blue indicator method, and the visual dye is used for detection. The color change in visible light will occur, which is conducive to the formation of a direct detection method of "sample in-result out". Compared with the pH color development method, the detection device is not easily disturbed during use and has higher sensitivity. Compared with the fluorescence method, the detection device does not need to be used with other professional instruments and has low cost.

[0037] The above shows and describes the basic principle, main features and advantages of the utility model. Those skilled in the art should understand that the utility model is not limited by the above embodiments, and the above embodiments and descriptions are only for explaining the principle of the utility model. Without departing from the spirit and scope of the utility model, the utility model may have various changes and improvements, and these changes and improvements fall within the scope of the utility model to be protected.

Claims

1. A portable nucleic acid molecular diagnostic device, characterized in that: The invention comprises a box body (100), a liquid separation reaction component (200) and a main control circuit component (300), wherein the box body (100) comprises an upper shell (101) and a lower cover (102), wherein the upper shell (101) is fixedly connected to the lower cover (102), wherein a sample addition hole (1011) is arranged on the upper shell (101), a status display (1012) is arranged on the upper shell (101), a button (1013) is arranged on the top of the upper shell (101), and the upper shell (101) comprises a detachable part (1015) and a fixed base (1016). 16), the detachable part (1015) and the fixed base (1016) are detachable, the detachable part (1015) is provided with a connecting part (10151), the connecting part (10151) is provided with an electronic contact point (10152), a position detection sensor (10153) is provided in the middle of one side of the connecting part (10151), a connecting port (10161) is provided on one side of the fixed base (1016), and the liquid separation reaction component (200) is arranged below the detachable part (1015).

2. A portable nucleic acid molecular diagnostic device according to claim 1, characterized in that: The liquid separation reaction component (200) comprises a liquid conducting groove (201), a liquid separation structure (202), a sealing gasket (203), a color reaction layer (204), a temperature adjustment unit (205) and a base (206); the liquid separation structure (202) is arranged at the bottom of the liquid conducting groove (201); the color reaction layer (204) is arranged at the bottom of the liquid separation structure (202); the sealing gasket (203) is arranged between the color reaction layer (204) and the liquid separation structure (202); the temperature adjustment unit (205) is arranged at the bottom of the color reaction layer (204); the base (206) is arranged at the bottom of the temperature adjustment unit (205); and a plurality of reaction chambers are provided at the top of the color reaction layer (204).

3. A portable nucleic acid molecular diagnostic device according to claim 1, characterized in that: The main control circuit component (300) comprises a main control component (301), a temperature control component (302), a detection light control component (303), a color sensor (304), a display component (305) and a signal transmission component (306); the main control component (301) is electrically connected to the temperature control component (302), the detection light control component (303) is electrically connected to the main control component (301), the color sensor (304) is electrically connected to the main control component (301), the display component (305) is electrically connected to the main control component (301), and the signal transmission component (306) is electrically connected to the main control component (301).

4. A portable nucleic acid molecular diagnostic device according to claim 2, characterized in that: The sealing gasket (203) is made of silicone rubber.

5. A portable nucleic acid molecular diagnostic device according to claim 1, characterized in that: The status display (1012) and the button (1013) may be combined into a display device (1014).

6. A portable nucleic acid molecular diagnostic device according to claim 1, characterized in that: The in-place detection sensor (10153) is a Hall sensor or a proximity sensor.

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