Oscillating frame for stem cell culture

Abstract

The utility model relates to the related technical field of biological culture, and discloses an oscillation frame for stem cell culture, which comprises a culture box, water absorption grooves are fixedly connected to two ends of the culture box, a heating box is fixedly connected outside the water absorption grooves, a water inlet is arranged between the water absorption grooves and the culture box, and a water outlet is arranged between the heating box and the culture box. A connecting port is formed between the water absorption tank and the heating box, one-way valve assemblies are arranged in the water inlet and the water inlet, a heating wire is fixedly connected into the heating box, and a piston plate is slidably connected into the water absorption tank; pure water in the incubator is sucked and heated in a reciprocating mode through reciprocating motion of the oscillating frame in the reciprocating oscillating process and then discharged, so that circulating suction and guiding-out of water in the incubator are achieved, rapid mixing of cold water and hot water in the incubator is achieved, and the practicability of the incubator is improved.

Description

Technical Field

[0001] The utility model relates to the technical field related to biological culture, in particular to an oscillation rack for stem cell culture. Background Art

[0002] Stem cells are a type of cells that are not fully differentiated and immature, but have the potential to regenerate various tissues, organs and the human body. They are called "universal cells" in the medical field. Stem cell culture medium is a special culture environment required for the culture of stem cells to achieve the adhesion or growth effect of stem cells. However, during the production process, traditional stem cell culture medium is prone to precipitation when left still for a long time, resulting in uneven reaction and affecting the culture effect of stem cells. Therefore, it is necessary to use an oscillating rack to shake the stem cell culture medium.

[0003] However, during the use of the shaking rack in the prior art, the culture tubes are usually heated by water bath heating. However, due to the limited flow rate of water in the incubator, the hot water in the incubator cannot be quickly mixed, resulting in uneven heat distribution in the incubator, and further resulting in different culture speeds in different culture tubes. Therefore, a shaking rack for stem cell culture is proposed. Utility Model Content

[0004] The purpose of the utility model is to provide a shaking rack for stem cell culture to solve the problems raised in the above background technology.

[0005] In order to achieve the above purpose, the utility model provides the following technical solutions:

[0006] A shaking frame for stem cell culture comprises an incubator, water suction grooves are fixedly connected at both ends of the incubator, a heating box is fixedly connected outside the water suction groove, a water inlet is arranged between the water suction groove and the incubator, a water outlet is arranged between the heating box and the incubator, a connecting port is arranged between the water suction groove and the heating box, a one-way valve assembly is arranged inside the water inlet and the water inlet, a heating wire is fixedly connected inside the heating box, a piston plate is slidably connected inside the water suction groove, a first spring is arranged on one side of the piston plate, a telescopic rod is fixedly connected on the other side of the piston plate, the telescopic rod passes through the water suction groove and is fixedly connected to a connecting frame arranged in the incubator, the connecting frame is fixedly connected to an shaking frame, a plurality of groups of slots distributed at equal distances are arranged in the shaking frame, and a crank slider assembly is connected to the piston plate.

[0007] As a further solution of the utility model: the crank slider assembly includes a sliding cylinder fixedly connected in the water suction trough, a driving rod is slidably connected in the sliding cylinder, one end of the driving rod is fixedly connected to the piston plate, the incubator is fixedly connected to a mounting plate, the mounting plate is fixedly connected to a mounting frame, the mounting frame is fixedly connected to a motor, one end of the crank is fixedly connected to the output shaft of the motor, the other end of the crank is rotatably connected to one end of a rocker arm, and the other end of the rocker arm is rotatably connected to the other end of the driving rod.

[0008] As a further solution of the utility model: a mounting groove is arranged on the top of the incubator, and a box cover is installed in the mounting groove.

[0009] As a further solution of the utility model: a lock buckle is rotatably connected to the outer wall of the incubator, and a hook is fixedly connected to the box cover, and the hook and the lock buckle cooperate with each other.

[0010] As a further solution of the utility model: the one-way valve assembly includes a sealing valve fixedly connected to the water inlet and the water inlet, a sealing ball is arranged in the sealing valve, a fixing frame is fixedly connected to the sealing valve, a sliding rod is slidably connected to the fixing frame, a top block is fixedly connected to the sliding rod, the top block and the sealing ball cooperate with each other, and a second spring mounted on the sliding rod is arranged between the top block and the fixing frame.

[0011] Compared with the prior art, the utility model has the following beneficial effects: the utility model reciprocatingly draws and heats the pure water in the incubator through the reciprocating motion of the oscillation frame during the reciprocating oscillation process, and then discharges it, thereby realizing the circulation of water in the incubator, and then realizing the rapid mixing of cold water and hot water in the incubator, thereby improving the practicability of the equipment. BRIEF DESCRIPTION OF THE DRAWINGS

[0012] Figure 1 The utility model is a schematic diagram of the structure of a shaking rack for stem cell culture.

[0013] Figure 2 This is a cross-sectional view of a shaking rack for stem cell culture in the utility model.

[0014] Figure 3 The utility model is a cross-sectional view of a shaking rack for stem cell culture.

[0015] Figure 4 for Figure 3 A partial enlarged view of point A in the middle.

[0016] In the figure: 1-incubator, 2-mounting slot, 3-box cover, 4-lock, 5-hook, 6-water absorption slot, 7-piston plate, 8-first spring, 9-telescopic rod, 10-connecting frame, 11-oscillation frame, 12-slot, 13-heating box, 14-connecting port, 15-heating wire, 16-water outlet, 17-water inlet, 18-sliding cylinder, 19-driving rod, 20-mounting plate, 21-mounting frame, 22-motor, 23-crank, 24-rocker arm, 25-sealing valve, 26-sealing ball, 27-fixed frame, 28-sliding rod, 29-top block, 30-second spring. DETAILED DESCRIPTION

[0017] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0018] See also Figure 1 to Figure 4In an embodiment of the utility model, a shaking frame for stem cell culture includes an incubator 1, water suction grooves 6 are fixedly connected at both ends of the incubator 1, a heating box 13 is fixedly connected to the outside of the water suction groove 6, a water inlet 17 is arranged between the water suction groove 6 and the incubator 1, a water outlet 16 is arranged between the heating box 13 and the incubator 1, a connecting port 14 is arranged between the water suction groove 6 and the heating box 13, a one-way valve assembly is arranged in the water inlet 17 and the water inlet 17, a heating wire 15 is fixedly connected inside the heating box 13, a piston plate 7 is slidably connected in the water suction groove 6, a first spring 8 is arranged on one side of the piston plate 7, a telescopic rod 9 is fixedly connected on the other side of the piston plate 7, the telescopic rod 9 passes through the water suction groove 6 and is fixedly connected to a connecting frame 10 arranged in the incubator 1, a shaking frame 11 is fixedly connected to the connecting frame 10, a plurality of groups of slots 12 distributed equidistantly are arranged in the shaking frame 11, the piston A crank slider assembly is connected to the plate 7. When the utility model is in use, the culture tube is first inserted into the slot 12, and then pure water is injected into the incubator 1. Then, the piston plate 7 is driven to reciprocate and extend through the crank slider assembly. The piston plate 7 drives the connecting frame 10 to reciprocate and extend through the telescopic rod 9. The connecting frame 10 drives the culture tube to reciprocate and oscillate through the oscillation frame 11. At the same time, when the piston plate 7 moves in the direction away from the water inlet 17, the water in the incubator 1 is sucked into the water absorption groove 6. Then, when the piston plate 7 moves toward the water inlet 17, the one-way valve assembly in the water inlet 17 is closed, so that the water in the water absorption groove 6 is injected into the heating box 13 along the connecting port 14, so that the water in the heating box 13 is heated through the heating wire 15, and the heated hot water is discharged into the incubator 1 along the water outlet 16, so that the culture tube in the incubator 1 is cultured at a constant temperature.

[0019] In one case of this embodiment, see Figure 1 to Figure 4 The crank slider assembly includes a sliding cylinder 18 fixedly connected to the water suction groove 6, a driving rod 19 is slidably connected in the sliding cylinder 18, one end of the driving rod 19 is fixedly connected to the piston plate 7, a mounting plate 20 is fixedly connected to the incubator 1, a mounting frame 21 is fixedly connected to the mounting plate 20, a motor 22 is fixedly connected to the mounting frame 21, one end of a crank 23 is fixedly connected to the output shaft of the motor 22, the other end of the crank 23 is rotatably connected to one end of a rocker arm 24, the other end of the rocker arm 24 is rotatably connected to the other end of the driving rod 19, the crank 23 slider assembly drives the crank 23 to rotate through the motor 22, the crank 23 drives the rocker arm 24 to reciprocate and extend, and the rocker arm 24 drives the driving rod 19 to reciprocate and extend.

[0020] In one case of this embodiment, see Figure 1 to Figure 4The top of the incubator 1 is provided with a mounting groove 2, a box cover 3 is installed in the mounting groove 2, a lock buckle 4 is rotatably connected to the outer wall of the incubator 1, a hook 5 is fixedly connected to the box cover 3, and the hook 5 and the lock buckle 4 cooperate with each other. The utility model installs the box cover 3 through the mounting groove 2, and realizes the fixed connection between the box cover 3 and the incubator 1 through the mutual cooperation of the hook 5 and the lock buckle 4, so that the incubator 1 is sealed and protected by the box cover 3.

[0021] In one case of this embodiment, see Figure 1 to Figure 4 The one-way valve assembly includes a sealing valve 25 fixedly connected to the water inlet 17 and the water inlet 17, a sealing ball 26 is arranged in the sealing valve 25, a fixing frame 27 is fixedly connected to the sealing valve 25, a sliding rod 28 is slidably connected to the fixing frame 27, a top block 29 is fixedly connected to the sliding rod 28, the top block 29 and the sealing ball 26 cooperate with each other, and a second spring 30 sleeved on the sliding rod 28 is arranged between the top block 29 and the fixing frame 27. The utility model performs one-way sealing on the water inlet 17 and the water inlet 17 through the mutual cooperation of the sealing ball 26 and the sealing valve 25. When the water pressure pushes the sealing ball 26 to move in a direction away from the sealing valve 25, the sealing ball 26 compresses the second spring 30 through the top block 29, thereby making the sealing ball 26 away from the sealing valve 25, thereby opening the sealing valve 25.

[0022] The working principle of the utility model is as follows: when the utility model is used, the culture tube is first inserted into the slot 12, and then pure water is injected into the incubator 1, and then the crank 23 is driven by the motor 22 to rotate, the crank 23 drives the rocker arm 24 to reciprocate, the rocker arm 24 drives the drive rod 19 to reciprocate, the drive rod 19 drives the piston plate 7 to reciprocate, the piston plate 7 drives the connecting frame 10 to reciprocate through the telescopic rod 9, and the connecting frame 10 drives the culture tube to reciprocate through the oscillation frame 11. At the same time, when the piston plate 7 moves away from the water inlet 17, the water in the incubator 1 is sucked into the water absorption groove 6. Then, when the piston plate 7 moves toward the water inlet 17, the one-way valve assembly in the water inlet 17 is closed, so that the water in the water absorption groove 6 is injected into the heating box 13 along the connecting port 14, so that the water in the heating box 13 is heated by the heating wire 15, and the heated hot water is discharged into the incubator 1 along the water outlet 16, so that the culture tubes in the incubator 1 are cultured at a constant temperature.

[0023] The above shows and describes the basic principle and main features of the utility model and the advantages of the utility model. For those skilled in the art, it is obvious that the utility model is not limited to the details of the above exemplary embodiments, and can be implemented in other specific forms without departing from the spirit or basic features of the utility model. Therefore, no matter from which point of view, the embodiments should be regarded as exemplary and non-restrictive. The scope of the utility model is limited by the attached claims rather than the above description, so it is intended to include all changes within the meaning and scope of the equivalent elements of the claims in the utility model. Any figure mark in the claims should not be regarded as limiting the claims involved.

[0024] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A shaking rack for stem cell culture, comprising an incubator, characterized in that: Water suction grooves are fixedly connected at both ends of the incubator, and a heating box is fixedly connected outside the water suction groove. A water inlet is arranged between the water suction groove and the incubator, a water outlet is arranged between the heating box and the incubator, a connecting port is arranged between the water suction groove and the heating box, and a one-way valve assembly is arranged in both the water inlet and the water inlet. A heating wire is fixedly connected in the heating box, a piston plate is slidably connected in the water suction groove, a first spring is arranged on one side of the piston plate, and a telescopic rod is fixedly connected on the other side of the piston plate. The telescopic rod passes through the water suction groove and is fixedly connected to a connecting frame arranged in the incubator, an oscillation frame is fixedly connected to the connecting frame, a plurality of groups of equidistantly distributed slots are arranged in the oscillation frame, and a crank slider assembly is connected to the piston plate.

2. The shaking rack for stem cell culture according to claim 1, characterized in that: The crank slider assembly includes a sliding cylinder fixedly connected in the water absorption trough, a driving rod slidably connected in the sliding cylinder, one end of the driving rod is fixedly connected to the piston plate, a mounting plate is fixedly connected to the incubator, a mounting frame is fixedly connected to the mounting plate, a motor is fixedly connected to the mounting frame, one end of the crank is fixedly connected to the output shaft of the motor, the other end of the crank is rotatably connected to one end of a rocker arm, and the other end of the rocker arm is rotatably connected to the other end of the driving rod.

3. The shaking rack for stem cell culture according to claim 1, characterized in that: The top of the incubator is provided with an installation groove, and a box cover is installed in the installation groove.

4. The shaking rack for stem cell culture according to claim 3, characterized in that: A lock buckle is rotatably connected to the outer wall of the incubator, and a hook is fixedly connected to the box cover, and the hook and the lock buckle cooperate with each other.

5. The shaking rack for stem cell culture according to claim 1, characterized in that: The one-way valve assembly includes a sealing valve fixedly connected to the water inlet and the water inlet, a sealing ball is arranged in the sealing valve, a fixing frame is fixedly connected to the sealing valve, a sliding rod is slidably connected to the fixing frame, a top block is fixedly connected to the sliding rod, the top block and the sealing ball cooperate with each other, and a second spring mounted on the sliding rod is arranged between the top block and the fixing frame.

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