Plant carbon sink real-time monitoring experimental device

Abstract

A plant carbon sink real-time monitoring experiment device comprises a plurality of culture boxes which are arranged in an arrayed mode, the inner space of each culture box is divided into an outer culture container and an inner culture container which are mutually independent, the inner culture containers are arranged on the lower portions in the outer culture containers, and sealed spaces are reserved on the upper portions in the outer culture containers. The outer culture container is communicated with an outer air inlet pipe, an outer air outlet pipe and an outer water conveying pipe, an adjustable LED light source for providing illumination is arranged on the outer culture container, the adjustable LED light source is connected with a voltage-adjustable power supply, and a gas concentration detection device and a temperature and humidity detection device are arranged in the outer culture container; the inner cultivation container is communicated with an inner air inlet pipe, an inner air outlet pipe and an inner water conveying pipe; the inner cultivation container comprises a lower container body and an upper container body which are vertically sealed, the lower container body is of a barrel body structure with an opening in the top, and the upper container body comprises a bottom wrapping opening matched with the edge of the opening in the top of the lower container body in a sealing mode, an elastic connecting part with a cavity formed in the middle and an elastic wrapping opening located at the top end.

Description

Technical Field

[0001] The utility model relates to the technical field of plant cultivation, in particular to a plant carbon sink real-time monitoring experimental device. Background Art

[0002] In order to cope with the environmental problem of global warming, improving the carbon fixation capacity of plants is one of the ways to reduce greenhouse gas emissions. Plant photosynthesis converts CO2 into a variety of organic matter and fixes it in the body, providing materials and energy for its growth. It is a basic link in the growth and development process and has great carbon sequestration capacity.

[0003] In order to achieve carbon peak before the scheduled deadline, it is necessary to develop carbon fixation technology, which includes physical carbon fixation and biological carbon fixation. Physical carbon fixation is the long-term storage of carbon dioxide in exploited oil and gas wells, coal seams and deep seas; biological carbon fixation is the conversion of inorganic carbon, namely carbon dioxide in the atmosphere, into organic carbon, namely carbohydrates, through natural vegetation, and fixes it in plants or soil. Natural carbon fixation can not only reduce the carbon content in the air, but also increase the oxygen content in the air, which is more advantageous than physical carbon fixation.

[0004] Land resources within cities are precious and the available green space is limited. Therefore, in order to improve the carbon sequestration capacity of plants within cities, it is necessary to study the carbon sequestration capacity of vegetation, so as to select plants with better carbon sequestration capacity from a large number of plant varieties for planting. At the same time, it is also necessary to understand the factors affecting the carbon sequestration capacity of plants. However, there is currently a lack of detection equipment for detecting the carbon sequestration capacity of trees.

[0005] In view of this, in order to experimentally detect the influencing factors of plant carbon fixation ability, the utility model provides a plant carbon sink real-time monitoring experimental device. Utility Model Content

[0006] The utility model aims to solve the problems existing in the above-mentioned prior art and provides a plant carbon sink real-time monitoring experimental device, which can carry out different plant cultivation experiments by changing different light intensities and different living environment parameters, and then understand the influencing factors of plant carbon fixation ability to meet different needs in teaching and practice.

[0007] The utility model solves the technical problem by adopting a technical solution: the plant carbon sink real-time monitoring experimental device comprises a plurality of culture boxes arranged in an array, the internal space of the culture box is divided into an outer culture container and an inner culture container which are arranged in independent spaces, the inner culture container is placed in the lower part of the outer culture container, a sealed space is reserved in the upper part of the outer culture container, the outer culture container is connected with an outer air inlet pipe, an outer air outlet pipe and an outer water pipe, an adjustable LED light source for providing illumination is arranged on the outer culture container, the adjustable LED light source is connected with an adjustable voltage power supply, a gas concentration detection device and a temperature and humidity detection device are arranged in the outer culture container; the inner culture container is connected with an inner air inlet pipe, an inner air outlet pipe and an inner water pipe;

[0008] The inner cultivation container includes a lower container body and an upper container body which are sealed up and down. The lower container body is a barrel structure with an open top. The upper container body includes a bottom wrapping opening which is sealed and fitted on the open edge of the top of the lower container body, an elastic connecting part which forms a cavity in the middle, and an elastic wrapping opening located at the top.

[0009] Preferably, the incubator comprises a box body with a placement opening at the front end and a sealed door sealed on the outside of the placement opening, mounting holes are provided on the left and right sides of the rear end of the box body, and a positioning connecting plate sealingly connected to the outer wall of the incubator and surrounding the mounting hole is provided on the outside of the mounting hole, and the positioning connecting plate is sealed with pipe joints respectively connected to the external air inlet pipe, the external air outlet pipe, the external water pipe, the internal air inlet pipe, the internal air outlet pipe and the internal water pipe.

[0010] Preferably, the external air inlet pipe is arranged on the left side of the box body, and the external air outlet pipe is arranged on the right side of the box body. The lower ends of the external air inlet pipe and the external air outlet pipe are provided with gas driving devices located inside the box body. The gas driving device at the left end drives the gas to flow from bottom to top, and the gas driving device at the right end drives the gas to flow from top to bottom.

[0011] Preferably, the gas driving device includes a driving fan located in the box body and a supporting positioning portion installed at the lower end of the driving fan for forming a flow space below the driving fan.

[0012] Preferably, a controller installed in the box is provided between the left and right gas drive devices, the gas concentration detection device and the temperature and humidity detection device are installed on the controller, and the controller is also provided with a light sensor located at the upper end of the temperature and humidity detection device and arranged toward the LED light source.

[0013] Preferably, the outer air inlet pipe and the inner air inlet pipe are respectively connected to an air pump, the outer water pipe and the inner water pipe are driven to deliver water by a peristaltic pump, the air pump and the peristaltic pump are respectively electrically connected to a power supply through a relay, and the outer air inlet pipe and the inner air inlet pipe are both provided with an electromagnetic valve located between the box and the air pump.

[0014] Preferably, a contact portion is provided on the outer side of the front end of the box body and is arranged around the box body opening. The sealing door extends outward and is arranged corresponding to the size of the contact portion. A sealing portion is protruding on the front end surface of the contact portion and is used to achieve contact and sealing with the inner side surface of the sealing door. A detachable connecting structure is provided on the contact portion to press and fix the sealing door to the outer side of the sealing portion.

[0015] Preferably, a control box is also included, in which a plurality of placement spaces are horizontally divided by partition plates, an air pump is fixedly installed in the upper placement space, an interval support plate installed in the placement space is provided at the upper end of the air pump, a solenoid valve is fixedly connected to the upper end of the interval support plate and is arranged corresponding to the air pump, a control circuit board electrically connected to the relay, power supply and solenoid valve respectively is provided in the placement space, and a data storage device respectively arranged corresponding to the incubator is connected to the control circuit board.

[0016] Preferably, the adjustable LED light source is a light source with a wavelength of 1100nm-200nm.

[0017] The beneficial effects of the utility model are as follows: a plurality of culture boxes 7 for control experiments and relatively sealed are arranged in the utility model, an adjustable LED light source is arranged on the culture box, and air and water are introduced into the culture box to ensure the normal growth requirements of the plants, and the voltage can be adjusted by a voltage-regulating power supply to change the light intensity of the corresponding adjustable LED light source, and the internal space in the culture box is divided into two independent spaces which respectively surround the sealed stems and leaves and roots of the plants, thereby making the detection of the change in the carbon dioxide concentration on the outside of the stems and leaves more accurate, and thereby making the experimental device simultaneously used to detect the photocarbon fixation ability of plants under different conditions such as light intensity and photoperiod. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 It is a schematic diagram of the structure of the utility model;

[0019] Figure 2 It is a left view of the utility model;

[0020] Figure 3 is a schematic diagram of the structure of the incubator;

[0021] Figure 4 It is a schematic diagram of the structure of the inner cultivation container;

[0022] Figure 5 This is a schematic diagram of the structure of the control box.

[0023] Description of the accompanying drawings: 1. incubator, 1-1. box body, 1-2. sealed door, 2. outer culture container, 3. outer air inlet pipe, 4. outer air outlet pipe, 5. outer water pipe, 6. inner culture container, 6-1. lower container body, 6-2. upper container body, 6-2-1. bottom wrapped opening, 6-2-2. elastic connection part, 6-2-3. elastic wrapped opening, 7. adjustable LED light source, 8. inner air inlet pipe, 9. inner air outlet pipe, 10. inner water pipe Water pipe, 11. Mounting hole, 12. Positioning connecting plate, 13. Pipe joint, 14. Gas driving device, 15. Support positioning part, 16. Controller, 17. Light sensor, 18. Air pump, 19. Peristaltic pump, 20. Relay, 21. Solenoid valve, 22. Contact part, 23. Sealing part, 24. Power supply, 25. Control box, 26. Partition plate, 27. Spacer support plate, 28. Control circuit board, 29. Data storage device. DETAILED DESCRIPTION

[0024] The utility model is further described below in conjunction with the accompanying drawings:

[0025] The plant is divided into three parts from top to bottom: the first is the stem and leaves at the top that receive light, the second is the main trunk in the middle, and the third is the root tendon at the bottom. Among them, the stem and leaves at the top that receive light play a major role in plant photosynthesis.

[0026] See attached Figure 1-3 As shown: In order to study the influence on the carbon fixation capacity of plants, a plant carbon sink real-time monitoring experimental device is disclosed in this embodiment, which includes a plurality of culture boxes 1 arranged in an array, and a control test is carried out through the plurality of culture boxes 1 to clarify the corresponding influencing factors; the internal space of the culture box 1 is divided into an outer culture container 2 and an inner cultivation container 6 which are arranged in independent spaces; the outer culture container 2 and the inner cultivation container 6 in this embodiment are arranged in an inner and outer structure, that is, the inner cultivation container 6 is placed in the lower part of the outer culture container 2, and a sealed space is reserved in the upper part of the outer culture container 2, and during the experimental detection, the roots of the test plants are surrounded and sealed in the inner cultivation container 6, and the stems and leaves of the test plants are surrounded and sealed in the outer culture container 2, so that the stems and leaves and roots of the plants are separated for experimental detection;

[0027] Specifically, the inner cultivation container 6 is used to cultivate the test plants and relatively wrap and seal the roots of the plants and the cultivation substrate, and at the same time, the stems and leaves of the test plants are sealed and wrapped in the outer cultivation container 2; and in this embodiment, the outer cultivation container 2 and the inner cultivation container 6 form two independent cultivation spaces separated from each other; so that the stems, leaves and roots of the plants are respectively in two independent spaces, and the parameters in the independent spaces can be controlled and monitored separately, so as to avoid the mutual circulation of air in the two spaces when monitoring the stems, leaves or roots of the test plants during the test, thereby ensuring the accuracy of data detection in the independent spaces;

[0028] Specifically, in order to ensure the normal growth requirements of the test plants and to ensure that the parameter data of each control group before the test are consistent, the outer culture container 2 in this embodiment is connected with an outer air inlet pipe 3, an outer air outlet pipe 4 and an outer water pipe 5, and the outer air inlet pipe 3 is connected with an air pump 18. In this embodiment, the outside air is pumped into the outer culture container 2 by the air pump 18; the movable end of the outer water pipe 5 is arranged in a water tank and driven by a peristaltic pump to pump water into the outer culture container 2, and an adjustable LED light source 7 for providing illumination is provided on the outer culture container 2, and the adjustable LED light source 7 is connected with an adjustable voltage power supply, wherein the adjustable LED light source 7 in this embodiment is a light source with a wavelength of 1100nm-200nm; the adjustable LED light source 7 can specifically be one or more of red light, far red light, purple light, blue light, green light, white light, warm white light, cold white light, ultraviolet A, ultraviolet B and ultraviolet C, wherein ultraviolet A is ultraviolet light with a wavelength in the 320-400nm band; ultraviolet B is ultraviolet light with a wavelength of Ultraviolet rays in the 280-320nm band; ultraviolet rays C are ultraviolet rays with a wavelength in the 100-280nm band; a gas concentration detection device, a temperature and humidity detection device and an air pressure detection device are arranged in the outer culture container 2, wherein the gas concentration detection device may be a carbon dioxide concentration sensor, and the temperature and humidity detection device and the air pressure detection device may be combined into a temperature, humidity and air pressure sensor; when in use, in this embodiment, gas is injected into the outer culture container 2 through the outer air inlet pipe 3 to control the carbon dioxide concentration in the outer culture container 2, and water is transported into the incubator 1 through the outer water pipe 5 to control the humidity in the incubator 1, thereby ensuring that the carbon dioxide concentration and humidity in different incubators 1 are the same before conducting a test on the effect of different light intensities on the light carbon fixation ability of plants; and then after a period of illumination by the adjustable LED light source 7, the carbon fixation ability of the test plant under the corresponding parameter conditions can be reflected by the changes in the carbon dioxide concentration and humidity in the incubator 1;

[0029] Similarly, the inner cultivation container 6 in this embodiment is also connected with an inner air inlet pipe 8, an inner air outlet pipe 9 and an inner water pipe 10, through which air, water and fertilizer can be transported into the inner cultivation container 6, thereby ensuring the normal growth requirements of the test plants when the external cultivation container 2 is tested and monitored; similarly, the inner air inlet pipe 8 is also connected to an air pump, and the active end of the inner water pipe 10 is also set in the water tank and driven by a peristaltic pump. In order to monitor or adjust the carbon dioxide concentration, temperature and humidity or other parameters in the inner cultivation container 6, a detection sensor for the corresponding parameters can also be set in the inner cultivation container 6; thus, the device can more accurately control the parameters in the inner cultivation container 6 before conducting a test on the outer cultivation container 2, further ensuring the accuracy of the test; at the same time, it can also ensure that the parameters in the outer cultivation container 2 in the incubator 1 remain consistent, by controlling one of the parameters such as the carbon dioxide concentration, humidity, and water and fertilizer supply in the inner cultivation container 6 to correspondingly monitor the changes in the carbon dioxide concentration and humidity in the inner and outer cultivation containers 2 within a certain period of time, thereby reflecting whether the different parameter data in the inner cultivation container 6 (i.e., the external environment of the plant roots) will affect the carbon fixation capacity of the plant;

[0030] Further, such as Figure 3 and 4 As shown, the inner cultivation container 6 in this embodiment includes a lower container body 6-1 and an upper container body 6-2 which are sealed up and down. The lower container body 6-1 is a barrel structure with an open top. The cultivation substrate is put into the lower container body 6-1 from the open top and piled up and laid in the lower container body 6-1. The roots of the test plants are correspondingly planted in the cultivation substrate; the upper container body 6-2 corresponds to surround the open top of the lower container body 6-1 and the roots of the test plants to form a relatively sealed independent space; specifically, the upper container body 6-2 in this embodiment includes a bottom wrapping opening 6-2-1 that is sealed and matched on the open edge of the top of the lower container body 6-1, an elastic connecting portion 6-2-2 that forms a cavity in the middle, and an elastic wrapping opening 6-2-3 located at the top; as shown Figure 4 As shown, the upper container body 6-2 in this embodiment is made of elastic rubber material. When in use, the bottom wrapping opening 6-2-1 is elastically deformed outwards and then clamped and sealed on the outside of the open top of the lower container body 6-1. Similarly, the elastic wrapping opening 6-2-3 is deformed outwards and clamped and sealed on the outside of the trunk of the test plant, thereby achieving wrapping and sealing of the roots of the test plant through the upper container body 6-2.

[0031] The main trunk of the test plant passes through the elastic wrapping opening 6-2-3 and fits tightly with the elastic wrapping opening 6-2-3, so that the stem and leaf parts of the test plant at the upper end are located in the outer culture container 2, while the root and cultivation substrate at the lower end of the test plant are wrapped and sealed in the lower container body 6-1; so that the stem and leaf parts and rhizome parts of the plant are separated by the inner cultivation container 6 and are in two independent spaces;

[0032] Furthermore, Figure 3 As shown, the incubator 1 in this embodiment includes a box body 1-1 with a placement opening at the front end and a sealing door 1-2 sealed outside the placement opening. The sealing door 1-2 and the box body 1-1 in this embodiment surround a sealed internal space; further, in order to ensure the sealing of the internal space, a contact portion 22 arranged around the opening of the box body 1-1 is provided on the outside of the front end of the box body 1-1, and the sealing door 1-2 extends outward and is arranged corresponding to the size of the contact portion 22. A sealing portion 23 for contacting and sealing with the inner side surface of the sealing door 1-2 is protruded on the front end surface of the contact portion 22, and a sealing portion 23 for pressing and fixing the sealing door 1-2 on the sealing portion 23 is provided on the contact portion 22. 3. Specifically, the left side of the sealing door 1-2 in this embodiment is rotatably connected to the contact part 22 through a hinge, and the contact part 22 is sealed with the front end opening of the box body 1-1, and the sealing part 23 is an elastic sealing ring; wherein the detachable connection structure comprises screw holes 30 respectively correspondingly opened on the contact part 22 and the sealing door 1-2, and fixing screws 31 screwed into the screw holes 30 from the outside to the inside, and the screw holes 30 are opened on the outside of the sealing part 23; when sealing, the fixing screws 31 are screwed into the screw holes 30 in sequence, and then the sealing door 1-2 is pressed and sealed on the outside of the sealing part 23 during the process of the fixing screws 31 being gradually screwed in.

[0033] In order to facilitate intuitive observation of the plant conditions in the incubator 1, the incubator 1 is made of glass material in this embodiment, and the adjustable LED light source 7 is installed on the outer side of the upper end of the outer culture container 2, that is, the outer side of the upper end of the incubator 1; in order to facilitate the disassembly and connection of the pipeline and the processing and manufacturing of the incubator 1 while ensuring the sealing effect of the incubator 1, in this embodiment, mounting holes 11 are opened on the left and right sides of the rear end of the box body 1-1, and a positioning connecting plate 12 is provided on the outer side of the mounting hole 11, which is sealed and connected to the outer wall of the incubator 1 and surrounds the mounting hole 11. The positioning connecting plate 12 is installed with a pipeline joint 13 connected to the outer air inlet pipe 3, the outer air outlet pipe 4, the outer water pipe 5, the inner air inlet pipe 8, the inner air outlet pipe 9 and the inner water pipe 10 respectively; the pipeline joint 13 passes through The positioning connecting plate 12 is sealed and connected to the positioning connecting plate 12; when in use, the outer air inlet pipe 3, the outer air outlet pipe 4, and the outer water pipe 5 are respectively connected to the outer culture container 2 by a hose sleeved on the connecting end outside the pipe joint 13; at the same time, in order to realize the connection between the inner air inlet pipe 8, the inner air outlet pipe 9 and the inner water pipe 10 and the inner cultivation container 6, a pipe joint can be correspondingly provided on the inner cultivation container 6, and then the hose is first connected to the connecting end outside the pipe joint 13 on the positioning connecting plate 12, and then the pipe joint 13 on the positioning connecting plate 12 and the pipe joint on the inner cultivation container 6 are connected by other hoses, so that the inner air inlet pipe 8, the inner air outlet pipe 9 and the inner water pipe 10 are connected to the inner cultivation container 6 by a segmented connection.

[0034] The positioning connecting plate 12 in the embodiment is made of polycarbonate material. Since it is relatively difficult to make small holes in the glass culture box 1, and multiple small holes can easily cause the glass to break, it is relatively simple to make small holes on the polycarbonate positioning connecting plate 12. Therefore, in this embodiment, a large hole is made on the glass culture box 1 and then the pipe joint 13 is installed by sealing and installing the positioning connecting plate 12 with small holes, so that the processing of the culture box 1 is relatively convenient, and the installation and removal of the pipe joint 13 are also convenient.

[0035] like Figure 3 As shown, since the air in the closed environment does not flow, it will cause air stratification, and carbon dioxide will sink to the bottom of the outer culture container 2, thereby affecting the photosynthesis of the test plant and the detection of the carbon dioxide concentration detection device, and thus it is necessary to ensure the air flow in the incubator 1. In this embodiment, a gas driving device 14 is provided in the incubator 1, and thus the stratified air in the outer culture container 2 can be driven by the gas driving device 14 to mix and move during the test;

[0036] Furthermore, in this embodiment, two gas driving devices 14 may be provided, and the two gas driving devices 14 are respectively located on the left and right sides of the box body 1-1. The gas driving device 14 at the left end can drive the carbon dioxide deposited at the bottom to flow from bottom to bottom, while the gas driving device 14 at the right end makes the gas at the top flow from top to bottom, thereby making the air in the outer culture container 2 flow in a clockwise direction, so that the air in the outer culture container 2 is mixed more evenly;

[0037] Furthermore, in this embodiment, the outer air inlet pipe 3 is arranged on the left side of the box body 1-1, and the outgoing air pipe 4 is arranged on the right side of the box body 1-1, so that the gas driving device 14 is respectively located at the lower ends of the outer air inlet pipe 3 and the outgoing air pipe 4, and at the same time, the left end gas driving device 14 drives the gas to flow from bottom to top, and the right end gas driving device 14 drives the gas to flow from top to bottom; and then when the carbon dioxide in the outer culture container 2 is deposited, the left end gas driving device 14 can drive the carbon dioxide deposited at the bottom to flow from bottom to bottom, and the right end gas driving device 14 makes the top gas flow from top to bottom, and then The air in the outer culture container 2 is made to flow in a clockwise direction, and the outer air inlet pipe 3 on the left is made to take in air, so that the deposited carbon dioxide flows clockwise and mixes with the newly entered air, and the gas at the upper end first passes through the outer air inlet pipe 4 on the right when flowing clockwise, so that part of the gas with a low carbon dioxide content is discharged from the outer air inlet pipe 4, thereby correspondingly increasing the carbon dioxide concentration in the incubator 1; when it is necessary to maintain a higher carbon dioxide concentration in the outer culture container 2 or the inner culture container 6, the carbon dioxide gas can also be directly input through the outer air inlet pipe 3 and the inner air inlet pipe 8;

[0038] On the contrary, if it is necessary to reduce the carbon dioxide concentration in the incubator 1, the gas driving device 14 at the left end drives the gas to flow from top to bottom, while the gas driving device 14 at the right end drives the gas to flow from bottom to top, and then when carbon dioxide is deposited, the gas in the incubator 1 flows counterclockwise under the action of the gas driving device 14, and at the same time, the outer air inlet pipe 3 on the left side takes in air, and the carbon dioxide deposited at the lower end flows counterclockwise, so that part of the carbon dioxide gas is discharged from the outer air pipe 4 on the right side, thereby correspondingly reducing the carbon dioxide concentration in the incubator 1; when it is necessary to maintain a lower carbon dioxide concentration in the outer culture container 2 or the inner culture container 6, oxygen can also be directly input through the outer air inlet pipe 3 and the inner air inlet pipe 8;

[0039] Specifically, Figure 3As shown, the gas driving device 14 in this embodiment includes a driving fan located in the box body 1-1 and a supporting and positioning portion 15 installed at the lower end of the driving fan for forming a flow space below the driving fan. In this embodiment, the supporting and positioning portion 15 is installed at the lower end of the driving fan, so that the driving fan is installed at a certain height, and the installation positioning of the driving fan is achieved. At the same time, a flow space can be formed at the lower end of the driving fan, thereby achieving a directional flow of gas in the outer culture container 2. At the same time, leaving the flow space can also make the deposited carbon dioxide directly located at the lower end of the driving fan, which is convenient for the driving fan to directly and quickly drive the deposited carbon dioxide to flow, and avoid the deposited carbon dioxide from mixing with the upper air before passing through the external air inlet pipe 3 or the external air outlet pipe 4;

[0040] A controller 16 installed in the box 1-1 is provided between the left and right gas driving devices 14, and a gas concentration detection device and a temperature and humidity detection device are installed on the controller 16. Figure 3 As shown, in this embodiment, the carbon dioxide concentration sensor is arranged on the left side of the upper end of the controller 16, and the temperature, humidity and air pressure sensor is arranged at the center position of the upper end of the controller 16. The controller 16 is also provided with a light sensor 17 located at the upper end of the temperature and humidity detection device and arranged toward the LED light source; wherein, the controller 16 in this embodiment is arranged at the bottom of the box 1-1 and between the left and right gas driving devices 14, thereby relatively extending the flow path of the carbon dioxide deposited at the bottom of the box 1-1, thereby enabling the gas to flow in a directional manner and drive most of the carbon dioxide gas deposited at the bottom of the box 1-1 to flow;

[0041] Specifically, the air pump 18 and the peristaltic pump 19 in this embodiment are electrically connected to the power supply 24 through the relay 20, and the outer air inlet pipe 3 and the inner air inlet pipe 8 are both provided with a solenoid valve 21 located between the box body 1-1 and the air pump 18; the solenoid valve 21 is further provided on the outer air inlet pipe 3 and the inner air inlet pipe 8 to further confirm the gas supply signal, and then when it is necessary to fill the outer culture container 2 with air, it is necessary not only to start the air pump 18 but also to open the solenoid valve 21 so that the gas can pass into the outer culture container 2, thereby ensuring the accurate execution of the gas supply signal and the accuracy of the experimental results;

[0042] like Figure 1 and 5As shown, the experimental device in this embodiment also includes a control box 25, and a plurality of placement spaces are horizontally divided in the control box 25 by a partition plate 26. The air pump 18 is fixedly installed in the upper placement space, and an interval support plate 27 installed in the placement space is provided at the upper end of the air pump 18. The solenoid valve 21 is fixedly connected to the upper end of the interval support plate 27 and is arranged correspondingly to the air pump 18. The air pump 18 and the solenoid valve 21 are installed correspondingly, so that it is convenient to perform corresponding inspections on the air pump 18 and the solenoid valve 21 when a fault occurs; at the same time, the interval support plate 27 can be made of a metal heat-conducting material, so that it is convenient to dissipate heat from the air pump 18;

[0043] Among them, a control circuit board 28 electrically connected to the relay 20, the power supply 24 and the solenoid valve 21 is also provided in the placement space. In this embodiment, a protection box is provided on the outside of the control circuit board 28. The control circuit board 28 is connected to a data storage device 29 respectively corresponding to the incubator 1. The real-time data changes of various parameters in the incubator 1 can be recorded and stored through the data storage device 29, which is convenient for subsequent recording and research.

[0044] This embodiment also discloses a method for using a plant carbon sink real-time monitoring experimental device, comprising the following steps:

[0045] Step 1: Transplant the test plants with the same number and size of leaves into the lower container body 6-1 and ensure that the transplanted test plants survive, then pull the bottom wrapping opening 6-2-1 at the lower end of the upper container body 6-2 outward to cover the outside of the open top of the lower container body 6-1, so that the elastic wrapping opening 6-2-3 at the upper end of the upper container body 6-2 is pulled outward to cover the outside of the trunk of the test plant, and then the roots of the test plant are wrapped and sealed by the upper container body 6-2;

[0046] Step 2: Place the inner cultivation container 6 with the test plant in step 1 into the lower part of the outer cultivation container 2 and connect the inner air inlet pipe 8, the inner air outlet pipe 9 and the inner water pipe 10 to the inner cultivation container 6 respectively, close the sealing door 1-2 so that the inner space of the incubator 1 is sealed and divided into the outer cultivation container 2 and the inner cultivation container 6, so that the stem and leaf parts of the test plant are in the outer cultivation container 2, and the root parts of the test plant are in the inner cultivation container 6;

[0047] Step three: Ventilate the outer culture container 2 through the air pump 18 and the external air inlet pipe 3, the air pressure in the outer culture container 2 increases, so that part of the gas is discharged from the external air pipe 4, thereby changing the gas concentration in the outer culture container 2, thereby realizing the adjustment of the gas concentration in different culture boxes 1; similarly, ventilate the inner cultivation container 6 through the air pump 18 and the internal air inlet pipe 8 to adjust the gas concentration in the inner cultivation container 6; introduce water into the outer culture container 2 through the peristaltic pump 19 and the external water pipe 5, thereby changing the humidity in the outer culture container 2; similarly, change the humidity in the inner cultivation container 6 through the peristaltic pump 19 and the internal water pipe 10.

[0048] When it is necessary to detect the effect of light intensity on the photo-carbon fixation ability of plants, the fourth step can be performed: the light intensity of the adjustable LED light sources 7 on different culture boxes 1 is made different through the adjustable voltage power supply, and the humidity and carbon dioxide concentration in the culture box 1 are ensured to be the same through the external air inlet pipe 3 and the external water pipe 5; after a period of cultivation, the photo-carbon fixation ability of the plants is indirectly reflected by respectively detecting the changes in humidity and carbon dioxide concentration in the external culture container 2; at the same time, the device can also detect the carbon fixation ability of plants under different photoperiods; specifically, the working time of the adjustable LED light source is changed by controlling the switching time of the voltage regulating power supply, thereby changing the photoperiod, and finally, after a period of cultivation, the changes in humidity and carbon dioxide concentration in the external culture container 2 are respectively detected to indirectly reflect the photo-carbon fixation ability of the plants;

[0049] In this experimental device, the stems, leaves and roots of the test plants are respectively surrounded and sealed in different independent spaces, thereby avoiding the influence of the plant roots and the cultivation matrix when the light carbon fixation ability experiment of the plant stems and leaves is carried out, so as to ensure the accuracy of the experimental results; at the same time, this experimental device can also carry out control experiments under different parameter conditions. When it is necessary to carry out the influence of different wavelengths of light on the light carbon fixation ability of the plant, it is only necessary to make the adjustable LED light source 7 emit different red light or blue light in step three and then make the other parameters in the outer culture container 2 and the inner culture container 6 consistent; at the same time, this experimental device can also monitor and record the changes in carbon dioxide concentration and humidity outside the stems, leaves and roots of the plants respectively during the experiment; and then it can be experimentally detected whether the light carbon fixation ability of the plants is affected when the plant roots are in different carbon dioxide concentrations or humidity; at the same time, the outer culture container 2 and the inner culture container 6 can even be placed in different carbon dioxide concentrations or humidity, so as to further study whether there is an influence on the light carbon fixation ability of the test plants.

[0050] Although the present invention has been shown and described with reference to the preferred embodiments, it will be understood by those skilled in the art that various changes in form and details may be made therein within the scope of the claims.

Claims

1. A plant carbon sink real-time monitoring experimental device, the device comprising a plurality of culture boxes (1) arranged in an array, characterized in that: The internal space of the culture box (1) is divided into an outer culture container (2) and an inner culture container (6) which are arranged in independent spaces. The inner culture container (6) is placed in the lower part of the outer culture container (2). A sealed space is reserved in the upper part of the outer culture container (2). The outer culture container (2) is connected to an outer air inlet pipe (3), an outer air outlet pipe (4) and an outer water pipe (5). The outer culture container (2) is provided with an adjustable LED light source (7) for providing illumination. The adjustable LED light source (7) is connected to an adjustable voltage power supply. A gas concentration detection device and a temperature and humidity detection device are arranged in the outer culture container (2); and the inner culture container (6) is connected to an inner air inlet pipe (8), an inner air outlet pipe (9) and an inner water pipe (10). The inner cultivation container (6) comprises a lower container body (6-1) and an upper container body (6-2) which are sealed up and down, the lower container body (6-1) being a barrel structure with an open top, and the upper container body (6-2) comprising a bottom wrapping opening (6-2-1) sealably fitted on the open edge of the top of the lower container body (6-1), an elastic connecting portion (6-2-2) forming a cavity in the middle, and an elastic wrapping opening (6-2-3) located at the top.

2. The plant carbon sink real-time monitoring experimental device according to claim 1 is characterized by: The incubator (1) comprises a box body (1-1) having a placement opening at the front end and a sealing door (1-2) sealedly arranged outside the placement opening; the box body (1-1) has mounting holes (11) on the left and right sides of the rear end; a positioning connection plate (12) is provided outside the mounting hole (11) and is sealingly connected to the outer wall of the incubator (1) and surrounds the mounting hole (11); the positioning connection plate (12) is sealingly connected to a pipe joint (13) respectively connected to an external air inlet pipe (3), an external air outlet pipe (4), an external water pipe (5), an internal air inlet pipe (8), an internal air outlet pipe (9) and an internal water pipe (10).

3. A plant carbon sink real-time monitoring experimental device according to claim 2, characterized in that: The external air inlet pipe (3) is arranged on the left side of the box body (1-1), and the external air outlet pipe (4) is arranged on the right side of the box body (1-1). The lower ends of the external air inlet pipe (3) and the external air outlet pipe (4) are both provided with a gas driving device (14) located inside the box body (1-1). The gas driving device (14) at the left end drives the gas to flow from bottom to top, and the gas driving device (14) at the right end drives the gas to flow from top to bottom.

4. A plant carbon sink real-time monitoring experimental device according to claim 3, characterized in that: The gas driving device (14) comprises a driving fan located in the box body (1-1) and a supporting positioning portion (15) installed at the lower end of the driving fan and used to form a flow space below the driving fan.

5. The real-time monitoring experimental device for plant carbon sink according to claim 3 is characterized by: A controller (16) installed in the box (1-1) is provided between the gas driving devices (14); the gas concentration detection device and the temperature and humidity detection device are installed on the controller (16); and the controller (16) is also provided with a light sensor (17) located at the upper end of the temperature and humidity detection device and arranged toward the LED light source.

6. A plant carbon sink real-time monitoring experimental device according to claim 1, characterized in that: The outer air intake pipe (3) and the inner air intake pipe (8) are respectively connected to an air pump (18); the outer water delivery pipe (5) and the inner water delivery pipe (10) are driven to deliver water via a peristaltic pump (19); the air pump (18) and the peristaltic pump (19) are respectively electrically connected to a power source (24) via a relay (20); and the outer air intake pipe (3) and the inner air intake pipe (8) are both provided with a solenoid valve (21) located between the box body (1-1) and the air pump (18).

7. A plant carbon sink real-time monitoring experimental device according to claim 2, characterized in that: The outer side of the front end of the box body (1-1) is provided with a contact portion (22) arranged around a circumference of the box body opening; the sealing door (1-2) extends outward and is arranged corresponding to the size of the contact portion (22); a sealing portion (23) is protrudingly provided on the front end surface of the contact portion (22) for achieving contact and sealing with the inner side surface of the sealing door (1-2); and a detachable connection structure is provided on the contact portion (22) for pressing and fixing the sealing door (1-2) on the outer side of the sealing portion (23).

8. The plant carbon sink real-time monitoring experimental device according to claim 6 is characterized by: The invention also comprises a control box (25), wherein a plurality of placement spaces are horizontally divided by a partition plate (26) in the control box (25), the air pump (18) is fixedly installed in the placement space at the upper end, the upper end of the air pump (18) is provided with a spacing support plate (27) installed in the placement space, the solenoid valve (21) is fixedly connected to the upper end of the spacing support plate (27) and is arranged corresponding to the air pump (18), the placement space is provided with a control circuit board (28) electrically connected to the relay (20), the power supply (24) and the solenoid valve (21), respectively, and the control circuit board (28) is connected to a data storage device (29) arranged corresponding to the incubator (1).

9. The plant carbon sink real-time monitoring experimental device according to claim 1 is characterized by: The adjustable LED light source (7) is a light source with a wavelength of 1100nm-200nm.

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