Tissue culture box for genetic breeding of radishes

Abstract

The utility model discloses a tissue culture box for genetic breeding of radish, and relates to the technical field of tissue culture, the tissue culture box comprises a box body, a culture cavity arranged in the box body and a ventilation assembly fixedly arranged on the box body, the ventilation assembly comprises a through pipe communicated with the culture cavity, an HEPA filter fixedly arranged in the through pipe and a plurality of groups of ultraviolet lamps, the HEPA filter is closer to the box body than the ultraviolet lamp, and the ventilation assembly further comprises a reflective layer fixedly arranged on the inner wall of the through pipe in an attached mode; the device has the advantages that the HEPA filter and the ultraviolet lamp are respectively arranged in the through pipe, so that double air sterilization and detoxification treatment is provided when air is introduced between the culture cavity and the outside of the closed frame, and the treatment effect is improved; a light reflecting layer is arranged on the inner wall of the through pipe, so that light of the ultraviolet lamp is reflected to be fully distributed in the through pipe, and the sterilization and disinfection treatment effect is further improved.

Description

Technical Field

[0001] The utility model relates to the technical field of tissue culture, and more specifically to a tissue culture box for radish genetic breeding. Background Art

[0002] "Tissue culture box for radish genetic breeding" refers to a special equipment used for experimental operations such as plant in vitro culture, tissue culture, and cell culture when conducting radish genetic breeding research; this incubator can provide radish tissues or cells with strictly controlled sterile, constant temperature, constant humidity, suitable light and other environmental conditions to promote their growth, differentiation and regeneration, and facilitate scientific researchers to carry out genetic improvement, germplasm resource preservation, mutant screening, gene transformation and other work;

[0003] In order to ensure the sterility of the environment, the existing {publication (publication) number: CN212128117U, a microbial incubator for bioengineering} and {publication (publication) number: CN212753663U, an alpine potato tissue culture device} both propose to use "ultraviolet lamps" to disinfect the cultivation environment, but the air in the cultivation environment also needs to be sterilized and detoxified. Although a "HEPA filter" is proposed to perform physical interception and sterilization and detoxification of ventilation, the sterilization and detoxification effect of the air is still not good with such a setting alone. Utility Model Content

[0004] The purpose of the utility model is to solve the above technical problems and provide a tissue culture box for radish genetic breeding.

[0005] In order to achieve the above-mentioned purpose, the utility model specifically adopts the following technical solutions:

[0006] The utility model provides a tissue culture box for radish genetic breeding, comprising a box body, a culture cavity arranged in the box body and a ventilation component fixed on the box body;

[0007] The ventilation component comprises a through pipe communicated with the culture chamber, a HEPA filter fixed in the through pipe and a plurality of groups of ultraviolet lamps.

[0008] As a preferred technical solution of the present utility model, the HEPA filter is closer to the box body than the ultraviolet lamp.

[0009] As a preferred technical solution of the utility model, the ventilation component also includes a reflective layer adhered and fixed to the inner wall of the through pipe.

[0010] As a preferred technical solution of the utility model, the box body includes a base, a closing frame fixed to the top of the base, an opening and closing door installed on the closing frame, a plurality of groups of culture tanks arranged at the top of the base and a controller fixed to the outside of the base. The culture chamber is formed between the base and the closing frame, one end of the through pipe passes through the outside of the closing frame to communicate with the culture chamber, and a plurality of groups of culture tanks are arranged in the culture chamber.

[0011] As a preferred technical solution of the utility model, a plurality of groups of monitors, processors, fans and lighting lamps are respectively arranged in the culture chamber and are all fixedly mounted on the closed frame.

[0012] As a preferred technical solution of the utility model, a plurality of switch valves communicating with the culture chamber are fixedly mounted outside the closed frame.

[0013] As a preferred technical solution of the utility model, a base frame is fixedly installed at the bottom end of the base.

[0014] The beneficial effects of the utility model are as follows:

[0015] By respectively arranging a HEPA filter 32 and an ultraviolet lamp 33 in the through pipe 31, a double air sterilization and detoxification treatment is provided when ventilation is between the culture chamber 2 and the outside of the closed frame 12, thereby improving the treatment effect; and a reflective layer 34 is arranged on the inner wall of the through pipe 31 to reflect the light of the ultraviolet lamp 33 to enhance the coverage of the through pipe 31, thereby further improving the effect of the sterilization and disinfection treatment. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 It is a structural schematic diagram of the utility model.

[0017] Figure numerals: box body -1, culture chamber -2, ventilation component -3, monitor -4, processor -5, switch valve -6, fan -7, base frame -8, lighting lamp -9, base -11, closing frame -12, opening and closing door -13, culture tank -14, controller -15. DETAILED DESCRIPTION Embodiment 1:

[0018] like Figure 1 As shown, this embodiment proposes: a tissue culture box for radish genetic breeding, comprising a box body 1, a culture chamber 2 arranged in the box body 1, and a ventilation component 3 fixed on the box body 1;

[0019] The box body 1 includes a base 11, a closed frame 12 fixed to the top of the base 11, and a culture chamber 2 is formed between the base 11 and the closed frame 12 (as shown in the figure, the space between the inner side of the closed frame 12 and the top of the base 11 is the culture chamber 2).

[0020] An opening and closing door 13 installed on the closing frame 12 (as shown in the figure, it is arranged at the rear end of the closing frame 12 and is rotatably arranged by a hinge or the like to open and close the closing frame 12);

[0021] Several groups of cultivation tanks 14 are arranged at the top of the base 11, and several groups of cultivation tanks 14 are arranged in the cultivation chamber 2 {as shown in the figure, the cultivation tank 14 is at the top of the base 11, by selecting radish plants with excellent traits or characteristics to be improved as mother plants, selecting young and tender tissues (such as stem tips, leaves, root tips, etc.) on them, putting them into a solution containing a fungicide (such as sodium hypochlorite, alcohol, etc.) for surface disinfection, and then placing them into the cultivation tank 14};

[0022] A controller 15 fixedly mounted on the outside of the base 11 (the controller 15 is a PLC controller, which is connected to an external power source to supply power to electrical components);

[0023] One end of the through tube 31 passes through the outside of the closed frame 12 to communicate with the culture chamber 2 (as shown in the figure, the left end of the through tube 31 is fixed to the right end of the closed frame 12 and penetrates into the culture chamber 2);

[0024] The ventilation assembly 3 includes a through pipe 31 connected to the culture chamber 2, a HEPA filter 32 fixed in the through pipe 31, and a plurality of groups of ultraviolet lamps 33 (the interception effect of the HEPA filter 32 and the sterilization and disinfection effect of the ultraviolet lamps 33 provide a double air sterilization and detoxification process when the ventilation is between the culture chamber 2 and the outside of the closed frame 12);

[0025] The HEPA filter 32 is closer to the box 1 than the ultraviolet lamp 33 (as shown in the figure, the HEPA filter 32 is arranged on the left side of the ultraviolet lamp 33 to prevent the lamp from continuously irradiating directly into the culture chamber 2 and affecting the growth of the culture);

[0026] The culture chamber 2 is provided with several groups of monitors 4 (including temperature and humidity sensors, oxygen sensors, carbon dioxide sensors, etc.), processors 5, fans 7 and lighting lamps 9 (the lighting lamps 9 can be fluorescent lamps, etc., to provide lighting for cultivation) and they are all fixed to the closed frame 12;

[0027] Monitor 4 includes temperature and humidity sensors, oxygen sensors, carbon dioxide sensors, etc.;

[0028] The processor 5 includes a heating plate, a similar compression refrigeration system, a humidifier, an ultraviolet lamp (the ultraviolet lamp is used to sterilize and disinfect the culture chamber 2), etc.;

[0029] The closed frame 12 is externally fixed with a plurality of switch valves 6 communicating with the culture chamber 2 (the switch valves 6 are electric switch valves, etc., for connecting to external equipment for generating oxygen or carbon dioxide, such as an oxygen generator or a carbon dioxide generator).

[0030] As shown in the figure, the fans 7 are provided with two groups, one group is provided at the opening where the through pipe 31 communicates with the inside of the closed frame 12 to improve the ventilation effect, and the other group is provided in the culture chamber 2 to quickly blow the heat, cold air, moisture, oxygen, carbon dioxide, etc. therein;

[0031] A bottom frame 8 is fixedly mounted on the bottom end of the base 11 to provide overall support and stability. Embodiment 2:

[0032] like Figure 1 As shown, the difference from Example 1 is that the ventilation component 3 also includes a reflective layer 34 (the reflective layer 34 can be an aluminum film, etc.) fixed to the inner wall of the through pipe 31, which reflects the light of the ultraviolet lamp 33 to enhance the coverage inside the through pipe 31, thereby further improving the effect of the sterilization and disinfection treatment.

[0033] The control method of the present invention is automatic control through a controller. The control circuit of the controller can be realized by simple programming by technicians in this field. The provision of power is also common knowledge in this field. The present invention is mainly used to protect mechanical devices, so the present invention will no longer explain the control method and circuit connection in detail.

[0034] The above shows and describes the basic principles and main features of the utility model and the advantages of the utility model. For those skilled in the art, it is obvious that the utility model is not limited to the details of the above exemplary embodiments, and can be implemented in other specific forms without departing from the spirit or basic features of the utility model. Therefore, no matter from which point of view, the embodiments should be regarded as exemplary and non-restrictive. The scope of the utility model is limited by the attached claims rather than the above description, and it is intended to include all changes within the meaning and scope of the equivalent elements of the claims. Any figure mark in the claims should not be regarded as limiting the claims involved.

[0035] In addition, it should be understood that although the present specification is described according to implementation modes, not every implementation mode contains only one independent technical solution. This description of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each embodiment may also be appropriately combined to form other implementation modes that can be understood by those skilled in the art.

Claims

1. A tissue culture box for radish genetic breeding, comprising a box body (1), a culture chamber (2) arranged in the box body (1), and a ventilation component (3) fixedly arranged on the box body (1); It is characterized in that The ventilation assembly (3) comprises a through pipe (31) communicating with the culture chamber (2), a HEPA filter (32) fixed in the through pipe (31), and a plurality of groups of ultraviolet lamps (33).

2. The tissue culture box for radish genetic breeding according to claim 1, characterized in that: The HEPA filter (32) is closer to the housing (1) than the ultraviolet lamp (33).

3. The tissue culture box for radish genetic breeding according to claim 2, characterized in that: The ventilation assembly (3) further comprises a reflective layer (34) which is fixedly attached to the inner wall of the through pipe (31).

4. The tissue culture box for radish genetic breeding according to claim 3, characterized in that: The housing (1) comprises a base (11), a closing frame (12) fixedly mounted on the top of the base (11), an opening and closing door (13) mounted on the closing frame (12), a plurality of groups of culture tanks (14) arranged on the top of the base (11), and a controller (15) fixedly mounted on the outside of the base (11); a culture chamber (2) is formed between the base (11) and the closing frame (12); one end of a through pipe (31) passes through the outside of the closing frame (12) to communicate with the inside of the culture chamber (2); and a plurality of groups of culture tanks (14) are arranged in the culture chamber (2).

5. The tissue culture box for radish genetic breeding according to claim 4, characterized in that: A plurality of groups of monitors (4), processors (5), fans (7) and lighting lamps (9) are respectively arranged in the culture chamber (2), and are all fixedly mounted on the closing frame (12).

6. The tissue culture box for radish genetic breeding according to claim 5, characterized in that: A plurality of switch valves (6) connected to the culture chamber (2) are fixedly mounted outside the closing frame (12).

7. The tissue culture box for radish genetic breeding according to claim 6, characterized in that: A bottom frame (8) is fixedly mounted on the bottom end of the base (11).

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