Reagent mixing device for virus packaging

Abstract

The utility model relates to the technical field of reagent mixing, and discloses a reagent mixing device for virus packaging, which comprises an outer shell, a rotating disc is arranged in the outer shell, a test tube box is arranged on the rotating disc, and a pressing mechanism and a limiting mechanism which are used for fixing test tubes are arranged in the test tube box. Through the matching of the positioning block and the positioning rod, the test tube box can be conveniently mounted on the rotating disc, and then the rotating disc drives the test tube box to rotate, so that reagents in test tubes rotate at a high speed, and the mixing is realized. And the lower connecting disc is driven by the pressing rod to move upwards, so that the pressing block enters the storage groove, one end of the pressing block is attached to the wall of the test tube, and the test tube is fixed in the storage groove. The test tube is prevented from shaking during rotation and the equipment safety is improved.

Description

Technical Field

[0001] The utility model relates to the field of reagent mixing, in particular to a reagent mixing device for virus packaging. Background Art

[0002] Virus packaging refers to the process of cloning the target gene into a viral vector in vitro through certain means, so that the target gene can be expressed and delivered by the virus. Virus packaging technology is an indispensable technology in the fields of gene therapy and vaccine research.

[0003] When conducting relevant experiments, three reagents are generally required: packaging plasmid, auxiliary plasmid and viral plasmid, and these reagents need to be shaken and mixed. If they are mixed manually, it is difficult to ensure a completely uniform reaction, and it is impossible to mix the reagents uniformly in large quantities. Therefore, the applicant proposes a reagent mixing device for virus packaging. It can quickly and stably shake and mix the reagents. Utility Model Content

[0004] In order to solve the technical problem of rapid and stable shaking and mixing of reagents, the utility model provides a reagent mixing device for virus packaging.

[0005] The utility model is implemented by the following technical scheme: a reagent mixing device for virus packaging, comprising an outer shell, wherein the outer shell is provided with:

[0006] A rotating disk, the rotating disk is rotatably mounted inside the outer shell;

[0007] A test tube box, the test tube box is clamped on the rotating disk, and a plurality of storage slots are provided in the test tube box;

[0008] A clamping mechanism, which is arranged in the test tube box and includes a clamping block and a clamping rod, one end of which extends out of the test tube box, the clamping rod is used to control the movement of the clamping block, and the clamping block is used to fix the test tube;

[0009] A limiting mechanism is provided between the pressing rod and the test tube box, and the limiting mechanism is used to adjust the position of the pressing rod.

[0010] Through the above technical solution, the test tube is fixed by the clamping mechanism to prevent the test tube from shaking or even being thrown out during rotation and mixing. The limiting mechanism cooperates to ensure that the clamping block in the clamping mechanism can work stably without damaging the test tube.

[0011] As a further improvement of the above solution, a rotating shaft is fixedly connected to the bottom of the rotating disk, a motor is further connected to the lower end of the rotating shaft, and the rotating shaft and the outer shell are rotatably connected.

[0012] As a further improvement of the above solution, a circular enclosure is fixedly provided on the rotating disk, and a positioning rod and a positioning block are fixedly connected inside the enclosure. At the same time, holes corresponding to the positioning rod and the positioning block are opened at the bottom of the test tube box.

[0013] Through the above technical solution, the test tube box can be easily installed on the rotating disk through the cooperation of the cross-shaped positioning block and the four positioning rods, and when the rotating disk rotates, the positioning block and the positioning rods can fix the test tube box.

[0014] As a further improvement of the above solution, the clamping mechanism further includes:

[0015] A lower connecting plate, wherein the lower connecting plate is fixedly connected below the pressing rod;

[0016] An oblique connecting rod, one end of which is rotatably connected to the lower connecting plate, and the other end of which is rotatably connected to the pressing block.

[0017] As a further improvement of the above solution, a clamping hole is opened on the inner wall of the storage groove, and the clamping block is movably installed in the clamping hole.

[0018] Through the above technical solution, when the clamping rod and the lower connecting plate move up and down, the clamping block is driven to move through the cooperation of the oblique connecting rod and move in the clamping hole.

[0019] As a further improvement of the above solution, a cavity is provided in the test tube box, and the limiting mechanism is installed in the cavity. The limiting mechanism includes:

[0020] A limit block, wherein the limit block is fixedly connected to the clamping rod, and two sides of the limit block are arranged as hemispherical structures;

[0021] A limiting rod, the limiting rod is movably installed in the test tube box, and one end of the limiting rod close to the limiting block is set to be spherical;

[0022] A limit spring is sleeved on the outer side of the limit rod.

[0023] As a further improvement of the above solution, a cover plate is rotatably connected to the top of the outer shell.

[0024] Compared with the prior art, the beneficial effects of the present invention are:

[0025] The utility model facilitates the installation of the test tube box on the rotating disk through the cooperation of the positioning block and the positioning rod, and then the rotating disk drives the test tube box to rotate, so that the reagents in the test tube rotate at high speed, thereby achieving mixing. In addition, the clamping rod drives the lower connecting disk to move upward, so that the clamping block enters the storage tank, and one end of the clamping block fits the test tube wall to fix the test tube in the storage tank. The test tube is prevented from shaking during rotation, thereby improving the safety of the equipment. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 It is a schematic diagram of the overall structure of the utility model;

[0027] Figure 2 This is an exploded view of the internal structure of the outer shell of the utility model;

[0028] Figure 3 This is a cross-sectional view of the test tube box of the utility model from the first perspective;

[0029] Figure 4 For the utility model test tube box is trying;

[0030] Figure 5 This is a cross-sectional view of the test tube box of the utility model from a second viewing angle.

[0031] Explanation of main symbols: 1. Outer shell; 2. Rotating disk; 3. Rotating shaft; 4. Motor; 5. Enclosure; 6. Positioning rod; 7. Positioning block; 8. Clamping mechanism; 81. Lower connecting disk; 82. Clamping rod; 83. Oblique connecting rod; 84. Clamping block; 9. Limiting mechanism; 91. Limiting block; 92. Limiting rod; 93. Limiting spring; 10. Test tube box; 11. Storage hole; 12. Clamping hole; 13. Cover plate. DETAILED DESCRIPTION

[0032] The present invention is further described below in conjunction with the accompanying drawings and specific implementation methods. It should be noted that, under the premise of no conflict, the various embodiments or technical features described below can be arbitrarily combined to form a new embodiment.

[0033] Please combine Figure 1 - Figure 5 The reagent mixing device for virus packaging of the present embodiment comprises an outer shell 1, firstly, a cover plate 13 is rotatably mounted on the top of the outer shell 1, and a rotating disk 2 is rotatably mounted on the inner wall of the outer shell 1, wherein a rotating shaft 3 is fixedly connected to the bottom of the rotating disk 2, and the outer side of the rotating shaft 3 is rotatably connected to the outer shell 1 through a bearing ring, and the bottom is connected to a motor 4. The motor 4 drives the rotating disk 2 to rotate through the rotating shaft 3.

[0034] In addition, a circular enclosure 5 is fixedly connected to the upper surface of the rotating disk 2. Inside the enclosure 5, positioning rods 6 and positioning blocks 7 are arranged. The positioning blocks 7 are cross-shaped structures. There are four positioning rods 6 located on the outside of the positioning blocks 7. The positioning rods 6 and the positioning blocks 7 are both fixedly connected to the rotating disk 2.

[0035] A test tube box 10 is matched with the rotating disk 2, and a cross-shaped hole corresponding to the positioning block 7 and a hole corresponding to the positioning rod 6 are opened at the bottom of the test tube box 10. Through the cooperation of the positioning block 7, the four positioning rods 6 and the corresponding holes, the test tube box 10 is clamped on the rotating disk 2. In actual use, the diameter of the enclosure 5 can be set to be 5-10 mm larger than the diameter of the test tube box 10, and the test tube box 10 is located inside the enclosure 5.

[0036] In this embodiment, eight storage slots are provided at equal angles on the test tube box 10, and the storage slots are used to place test tubes. Because the rotating disk 2 needs to rotate when the reagents are mixed, in order to further fix the test tubes, a clamping mechanism 8 is also provided in the test tube box 10. Specifically, the clamping mechanism 8 includes a lower connecting disk 81 and a clamping rod 82. First, the lower connecting disk 81 is fixedly connected to the bottom of the clamping rod 82, and the upper end of the clamping rod 82 extends out of the test tube box 10. In addition, an oblique connecting rod 83 is rotatably connected to the lower connecting disk 81, and a clamping block 84 is rotatably connected to the other end of the oblique connecting rod 83.

[0037] A through clamping hole 12 is provided on the inner wall of the storage groove, and a clamping block 84 is movably installed in the clamping hole 12. The clamping block 84 is arranged in an arc shape at one end facing the test tube to facilitate fitting with the test tube wall. In addition, a protrusion is arranged on the side of the clamping hole 12 to limit the clamping block 84, so that the clamping block 84 can only move a limited distance along the direction of the clamping hole 12, thereby preventing the clamping block 84 from moving too long and damaging the test tube.

[0038] In the initial state, the oblique connecting rod 83 is in an inclined state, and the pressing block 84 is located in the pressing hole 12 (not entering the storage slot). When the pressing rod 82 moves upward, the inclined oblique connecting rod 83 gradually becomes horizontal, and at this time the oblique connecting rod 83 pushes the pressing block 84 to move into the storage slot, and one end of the pressing block 84 fits against the test tube wall and gradually presses the test tube into the storage slot.

[0039] Because the pressing block 84 is required to press the test tube all the time when the reagents are mixed, in order to keep the pressing block 84 always in a pressing state on the test tube, a cavity is opened in the test tube box 10, and a limiting mechanism 9 is arranged in the cavity.

[0040] Specifically, the limiting mechanism 9 includes a limiting block 91. First, the limiting block 91 is fixedly connected to the pressing rod 82, and hemispherical protrusions are arranged at both ends of the limiting block 91. In addition, the limiting mechanism 9 also includes a limiting rod 92. The end of the limiting rod 92 close to the limiting block 91 is provided with a spherical structure, and the outer sleeve of the limiting rod 92 is provided with a limiting spring 93. Under the push of the limiting spring 93, the limiting rod 92 approaches and fits the limiting block 91. The spherical and hemispherical structures on the limiting rod 92 and the limiting block 91 will fit and contact.

[0041] In the initial state, that is, when the clamping rod 82 is not pulled upward, the limit block 91 is in the lower half of the cavity. At this time, the limit rod 92 is located above the hemispherical structure on the side of the limit block 91, and the limit block 91 is blocked by the limit rod 92 group. When the clamping rod 82 moves upward, it drives the limit block 91 to move upward, and the side of the limit block 91 will first push the limit rod 92 to move away from the limit block 91, and the limit rods 92 on both sides move in the direction away from the middle limit block 91, and the limit spring 93 is compressed.

[0042] When the limit block 91 moves upward and moves to the top of the limit rod 92 (blocked by the cavity wall group of the test tube box 10), the limit rod 92 is reset under the restoring force of the limit spring 93. At this time, the limit rod 92 is located below the hemispherical structure on the side of the limit block 91, and presses against the limit block 91 from the bottom.

[0043] When the clamping rod 82 is pressed downward, the process is opposite to the above, the oblique connecting rod 83 changes from a horizontal state to an inclined state, and the clamping block 84 is retracted, which makes it convenient to take out the test tube.

[0044] The implementation process and principle of a reagent mixing device for virus packaging in the embodiment of the present application are as follows:

[0045] (I): Place the test tube containing the solution in the storage hole 11 of the test tube box 10, and then pull the clamping rod 82 upward. When the clamping rod 82 moves upward, the inclined diagonal connecting rod 83 gradually becomes horizontal, and the diagonal connecting rod 83 pushes the clamping block 84 to move into the storage slot, and one end of the clamping block 84 fits against the test tube wall and presses the test tube into the storage slot.

[0046] The clamping rod 82 moves upward and drives the limit block 91 to move upward. When the limit block 91 moves to the upper part of the limit rod 92, it is blocked. At this time, the limit block 91 cannot move downward, that is, the clamping rod 82 cannot move downward. The clamping block 84 always maintains a state of being in contact with the wall of the test tube, that is, the test tube is always fixed in the storage hole 11.

[0047] (ii): Through the positioning effect of the limit block 91 and the limit rod 92, the test tube box 10 is clamped on the rotating disk 2. Then the motor 4 is started, and the rotating disk 2 is driven by the motor 4 to rotate. The rotating disk 2 drives the test tube box 10 to rotate. The reagent solution in the test tube also rotates at a high speed to achieve mixing. Therefore, during the rotation process, the clamping block 84 always presses the test tube. Therefore, there is no need to worry about the test tube shaking or even being thrown out during rotation, which improves the safety of the equipment.

[0048] (III): After the mixing is completed, when the pressing rod 82 is pressed down, the oblique connecting rod 83 changes from a horizontal state to an inclined state, and the pressing block 84 is retracted and enters the pressing hole 12, so that the test tube can be easily taken out.

[0049] The above-mentioned implementation modes are only preferred implementation modes of the present invention, and cannot be used to limit the protection scope of the present invention. Any non-substantial changes and substitutions made by technicians in this field on the basis of the present invention shall fall within the scope of protection required by the present invention.

Claims

1. A reagent mixing device for virus packaging, comprising an outer shell, characterized in that: The outer shell is provided with: A rotating disk, the rotating disk is rotatably mounted inside the outer shell; A test tube box, the test tube box is clamped on the rotating disk, and a plurality of storage slots are provided in the test tube box; A clamping mechanism, which is arranged in the test tube box and includes a clamping block and a clamping rod, one end of which extends out of the test tube box, the clamping rod is used to control the movement of the clamping block, and the clamping block is used to fix the test tube; A limiting mechanism is provided between the pressing rod and the test tube box, and the limiting mechanism is used to adjust the position of the pressing rod.

2. The virus packaging reagent mixing device according to claim 1, characterized in that: The bottom of the rotating disk is fixedly connected with a rotating shaft, the lower end of the rotating shaft is also connected with a motor, and the rotating shaft is rotatably connected to the outer shell.

3. The virus packaging reagent mixing device according to claim 1, characterized in that: A circular enclosure is fixedly arranged on the rotating disk, and a positioning rod and a positioning block are fixedly connected inside the enclosure. Meanwhile, holes corresponding to the positioning rod and the positioning block are opened at the bottom of the test tube box.

4. The virus packaging reagent mixing device according to claim 1, characterized in that: The clamping mechanism also includes: A lower connecting plate, wherein the lower connecting plate is fixedly connected below the pressing rod; An oblique connecting rod, one end of which is rotatably connected to the lower connecting plate, and the other end of which is rotatably connected to the pressing block.

5. The virus packaging reagent mixing device according to claim 1, characterized in that: A pressing hole is provided on the inner wall of the storage groove, and the pressing block is movably installed in the pressing hole.

6. The virus packaging reagent mixing device according to claim 1, characterized in that: The test tube box is provided with a cavity, the limiting mechanism is installed in the cavity, and the limiting mechanism comprises: A limit block, wherein the limit block is fixedly connected to the clamping rod, and two sides of the limit block are arranged as hemispherical structures; A limiting rod, the limiting rod is movably installed in the test tube box, and one end of the limiting rod close to the limiting block is set to be spherical; A limit spring is sleeved on the outer side of the limit rod.

7. The virus packaging reagent mixing device according to claim 1, characterized in that: The top of the outer shell is also rotatably connected with a cover plate.

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