Cassava leaf pathogen inoculator

Abstract

The utility model relates to the technical field of inoculators, and discloses a cassava leaf pathogen inoculator which comprises a frame body and an injection assembly, the frame body is of a concave structure, a sliding hole is formed in one extending end of the frame body, a supporting part used for supporting the surface of the blade is arranged on the surface of the other extending end of the frame body, and the sliding hole is right opposite to the supporting part; the injection assembly comprises a bacterial liquid cylinder and a piston push head, the piston push head is arranged in the bacterial liquid cylinder in a sliding mode, and the peripheral surface of the bacterial liquid cylinder is tightly connected with the side wall of the sliding hole, so that the bacterial liquid cylinder is relatively static in a normal state and slides in the direction close to the supporting part in the sliding hole under the action of external force, and bacterial liquid grooves are formed in the surfaces of the blades. Thus, through the design that the injection assembly is sleeved with the frame body, and the frame body is slidably connected with the injection assembly, a researcher can conduct inoculation operation with one hand, operation is more convenient, and practicability is higher.

Description

Technical Field

[0001] The utility model belongs to the technical field of inoculators, and in particular relates to a cassava leaf pathogen inoculator. Background Art

[0002] Cassava ( Manihot esculenta Crantz ) is a plant of the Euphorbiaceae family, cassava, which is a very important food crop in subtropical and tropical countries and regions. It has the advantages of strong adaptability, easy planting, and high yield. However, cassava will be attacked by pathogens during its growth, which greatly limits its yield.

[0003] When studying the pathogenic mechanism of cassava, researchers usually inoculate cassava to observe the infection of the pathogen on cassava leaves. During the inoculation process, researchers usually use a syringe for injection inoculation, but in this way, the researcher needs to support the fingers of one hand on the upper surface of the leaf, and use the other hand to press the bacterial liquid groove on the lower surface of the leaf with a needleless syringe, and then inject the bacterial liquid into the bacterial liquid groove to complete the inoculation. During the inoculation process, this inoculation method requires two hands to work when pressing the bacterial liquid groove, which is inconvenient to operate. Therefore, the practicality of the existing inoculator is low. Utility Model Content

[0004] In order to solve the deficiencies of the prior art, the utility model provides a cassava leaf pathogen inoculator.

[0005] The technical effects to be achieved by the utility model are achieved through the following technical aspects:

[0006] A cassava leaf pathogen inoculator, comprising: a frame and an injection assembly;

[0007] The frame is configured as a concave structure, a sliding hole is provided on one extending end of the frame, a support portion for supporting the surface of the blade is provided on the surface of the other extending end of the frame, and the sliding hole is arranged opposite to the support portion;

[0008] The injection assembly includes a bacteria liquid cylinder and a piston pusher head, the piston pusher head is slidably arranged in the bacteria liquid cylinder, a rotating lever is arranged on the bacteria liquid cylinder, and the outer peripheral surface of the bacteria liquid cylinder is tightly connected to the side wall of the sliding hole so as to be relatively still under normal conditions. Under the action of external force, the bacteria liquid cylinder slides in the sliding hole toward the support portion so that a bacteria liquid groove appears on the surface of the blade.

[0009] In some embodiments, the bacterial liquid cylinder includes a metering end and a liquid outlet end, and a poking leaf portion is provided on an end surface of the liquid outlet end away from the metering end.

[0010] In some embodiments, the number of the poking leaf parts is set to at least two.

[0011] In some embodiments, a limiting portion for preventing the bacteria liquid cylinder from penetrating the leaves is provided on the bacteria liquid cylinder.

[0012] In some embodiments, the limiting portion is configured as a silicone sheet.

[0013] In some embodiments, a clamping portion is provided on the outer peripheral surface of the bacteria liquid cylinder protruding outward, and the clamping portion is movably clamped with the surface of the frame.

[0014] In some embodiments, when the engaging portion is engaged with the surface of the frame, the distance between the end surface of the bacteria liquid cylinder close to the supporting portion and the surface of the supporting portion is 0.05 mm to 0.08 mm.

[0015] In some embodiments, a frosted layer is disposed on the outer surface of the bacterial liquid cylinder.

[0016] In some embodiments, the sliding hole and the injection assembly may be provided as at least two.

[0017] In summary, the utility model has at least the following benefits:

[0018] The cassava leaf pathogen inoculator provided by the utility model has a certain spacing between the bacterial liquid cylinder and the support part before use, so as to facilitate the entry of the cassava leaf; when inoculating, the frame is held, and a turning force is applied to the cassava leaf through the frame, so that the lower surface of the cassava leaf faces upward, at this time, the cassava leaf is located between the support part and the bacterial liquid cylinder, and then the bottom end of the bacterial liquid cylinder is aligned with the position where the leaf needs to be inoculated, and the bacterial liquid cylinder is pressed down with the thumb, and a pressing effect is generated on the lower surface of the leaf through the bottom of the bacterial liquid cylinder, until a bacterial liquid groove is ground out of the lower surface of the leaf, and finally, the piston push head is pressed down with the thumb, so that the bacterial liquid can be injected into the bacterial liquid groove of the leaf. In this way, by sleeved with a frame on the outer side of the injection assembly, and by the design of sliding connection between the frame and the injection assembly, the researcher can perform the inoculation operation with one hand, and the operation is more convenient and more practical. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 It is a schematic diagram of the overall structure of the cassava leaf pathogen inoculator according to an embodiment of the utility model;

[0020] Figure 2 It is a schematic diagram of the overall structure of the injection assembly of an embodiment of the utility model;

[0021] Figure 3 It is a schematic diagram of the partial structure of the injection assembly of an embodiment of the utility model.

[0022] Markings in the figure:

[0023] 10. Cassava leaf pathogen inoculator;

[0024] 100, frame; 110, sliding hole; 120, support part;

[0025] 200, injection assembly; 210, bacterial liquid cylinder; 211, metering end; 212, liquid outlet end; 213, leaf poking part; 214, limiting part; 215, clamping part; 220, piston push head; 230, rotating lever. DETAILED DESCRIPTION

[0026] In order to make the purpose, technical solution and advantages of the implementation of the utility model clearer, the technical solution in the implementation of the utility model will be clearly and completely described below in conjunction with the drawings in the implementation of the utility model. The described implementation is a part of the implementation of the utility model, not all of the implementations.

[0027] Therefore, the following detailed description of the embodiments of the present invention provided in the accompanying drawings is not intended to limit the scope of the claimed invention, but merely represents selected embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in the field without creative work are within the scope of protection of the present invention.

[0028] In the following embodiments and drawings, reference is made to Figures 1 to 3 In the coordinate system, the direction indicated by the arrow of the X-axis is right, the direction indicated by the arrow of the Y-axis is forward, and the direction indicated by the arrow of the Z-axis is up.

[0029] It is understandable that during inoculation, the lower surface of the leaf is used to inoculate the corresponding bacterial solution, and the bacterial solution is selected according to specific research needs and is not limited here.

[0030] Embodiment 1:

[0031] like Figure 1 and Figure 2 As shown, in some embodiments, a cassava leaf pathogen inoculator 10 includes: a frame 100 and an injection assembly 200; the frame 100 is configured as a concave structure, a sliding hole 110 is opened on an extended end of the frame 100, and a support portion 120 for supporting the surface of a leaf is provided on the surface of the other extended end of the frame 100, and the sliding hole 110 is arranged opposite to the support portion 120; the injection assembly 200 includes a bacteria liquid cylinder 210 and a piston push head 220, the piston push head 220 is slidably arranged in the bacteria liquid cylinder 210, and the outer peripheral surface of the bacteria liquid cylinder 210 is tightly connected to the side wall of the sliding hole 110, so as to be relatively stationary under normal conditions, and under the action of external force, the bacteria liquid cylinder 210 slides in the sliding hole toward the direction close to the support portion 120, so that a bacteria liquid groove appears on the leaf surface.

[0032] Specifically, the frame 100 is a side-mounted concave-shaped structure, and a sliding hole 110 that runs through the top and bottom is opened on the top of the frame 100. The sliding hole 110 is used to accommodate the injection assembly 200, so that the injection assembly 200 can slide along the Z-axis direction under the guidance of the sliding hole 110 until it abuts against the lower surface of the blade; the upper side surface of the bottom end of the frame 100 is set as a support part 120, and the support part 120 is used to support and fix the lower surface of the blade. In addition, the width of a single finger of a person is generally 1 cm, and the inoculator sets the width of the support part 120 to at least 2 cm, so that the support area of ​​the blade is larger, thereby effectively preventing the blade from being easily damaged due to concentrated force. The injection assembly 200 is used to make a water stain-like mark on the lower surface of the blade and inject bacterial liquid into the mark to complete the inoculation, wherein the mark is set as a bacterial liquid tank, and the bacterial liquid cylinder 210 is arranged in cooperation with the sliding hole 110 so as to be stationary relative to each other under normal conditions and slide in the sliding hole 110 under a certain external force; the way in which the piston push head 220 and the bacterial liquid cylinder 210 are used in cooperation is a conventional technical means known to those skilled in the art, and will not be described here in detail.

[0033] It is worth noting that before use, there is a certain distance between the bacterial liquid cylinder 210 and the support part 120 to facilitate the entry of the cassava leaves; when inoculating, the frame 100 is held, and a turning force is applied to the cassava leaves through the frame 100, so that the lower surface of the cassava leaves faces upward. At this time, the cassava leaves are located between the support part 120 and the bacterial liquid cylinder 210, and then the bottom end of the bacterial liquid cylinder 210 is aligned with the position where the leaves need to be inoculated, and the bacterial liquid cylinder 210 is pressed down with the thumb, and the lower surface of the leaves is pressed by the bottom of the bacterial liquid cylinder 210 until the lower surface of the leaves is ground out of the bacterial liquid groove, and finally, the piston push head 220 is pressed down with the thumb, so that the bacterial liquid can be injected into the bacterial liquid groove of the leaves. In this way, the frame 100 is sleeved on the outer side of the injection component 200, and the frame 100 is slidably connected to the injection component 200, so that the researchers can perform the inoculation operation with one hand, which is more convenient to operate and more practical.

[0034] Example 2

[0035] This example is a further implementation of Example 1. It can be understood that the thickness of cassava leaves is generally about 0.1 mm to 0.3 mm. Young leaves are generally 0.1 mm and can be directly injected and inoculated, while old leaves are generally about 0.3 mm and it is difficult to form a bacterial liquid groove. Therefore, in order to facilitate the formation of a bacterial liquid groove on the old leaves, Figures 1 to 3 As shown, in this embodiment, the bacteria liquid cylinder 210 includes a metering end 211 and a liquid outlet end 212 , and a poking leaf portion 213 is provided on an end surface of the liquid outlet end 212 away from the metering end 211 .

[0036] Specifically, the leaf poking part 213 may be, but is not limited to, saw teeth. When the liquid outlet 212 is pressed down to contact the cassava leaves, the leaf poking part 213 can better poke into the leaves, facilitating the formation of the bacterial liquid tank. After pressing, the bacterial liquid cylinder 210 is rotated, thereby driving the leaf poking part 213 to rotate. In this way, it is easier to form the required bacterial liquid tank on the old leaves, thereby effectively increasing the practicality of the inoculator.

[0037] To facilitate the use of the inoculator, Figure 3 As shown, in some embodiments, the number of the poking leaf portions 213 is set to at least two.

[0038] Specifically, at least two poking leaf portions 213 are connected to the bottom end surface of the liquid outlet end 212, the number of the poking leaf portions 213 is optional but not limited to three, and the three poking leaf portions 213 are evenly spaced, so that three shallow marks can be pressed out on the leaves by pressing down the bacteria liquid cylinder 210, and then the bacteria liquid cylinder 210 can be rotated, so that the bacteria liquid tank can be made more quickly.

[0039] In addition, it is worth mentioning that in order to prevent the leaf-poking portion 213 from pressing down too much and piercing the cassava leaves, the height of the leaf-poking portion 213 needs to be controlled. Since the height of the leaf-poking portion 213 is relatively small, the leaf-poking portion 213 can be obtained by laser cutting the end surface of the liquid outlet end 212.

[0040] To avoid penetrating cassava leaves, e.g. Figures 1 to 3 As shown, in some embodiments, the bacterial liquid cylinder 210 is provided with a stopper 214 for preventing the blades from penetrating.

[0041] Specifically, a limiting portion 214 is provided on the outer peripheral side of the liquid outlet end 212, and the distance between the bottom surface of the limiting portion 214 and the bottom surface of the leaf-poking portion 213 can be set to be less than 0.1 mm. In this way, after the leaf-poking portion 213 is pressed down to a corresponding distance, the blade surface abuts against the bottom surface of the limiting portion 214, thereby preventing the leaf-poking portion 213 from continuing to press down and penetrate the blade.

[0042] In order to prevent the limiting portion 214 from damaging the blade, in some embodiments, the limiting portion 214 is configured to be made of a soft material.

[0043] Specifically, the material of the limiting portion 214 may be, but is not limited to, silicone, and the limiting portion 214 may be, but is not limited to, a silicone sheet, and the buffering effect of the silicone sheet can prevent damage to the blade surface. In another embodiment, the limiting portion 214 may be configured as a ring wound by an adhesive tape, which is more cost-effective and has a simpler manufacturing process.

[0044] In order to facilitate the removal of the injection assembly 200, as Figure 1 and Figure 2As shown, in some embodiments, the width of the limiting portion 214 is smaller than the width of the sliding hole 110 .

[0045] Specifically, the injection assembly 200 can be separated from the frame 100 from above. When the bacterial liquid is absorbed, a pulling force is applied to the bacterial liquid cylinder 210 to pull the injection assembly 200 out of the sliding hole 110, so that the injection assembly 200 can absorb the bacterial liquid easily. When the injection assembly 200 absorbs the bacterial liquid, in order to prevent the limiting portion 214 from being contaminated with the bacterial liquid, a hollow needle can be inserted into the liquid outlet 212, and the bacterial liquid can be absorbed through the hollow needle. After the bacterial liquid is filled, the needle can be removed.

[0046] In order to facilitate the production of bacterial liquid tanks on leaves, such as Figure 1 and Figure 2 As shown, in some embodiments, a rotating lever 230 is disposed on the bacteria liquid cylinder 210 .

[0047] Specifically, a rotating lever 230 is protruding outward from the top of the bacteria liquid cylinder 210, and the specific orientation of the rotating lever 230 conforms to the ergonomic setting. After the bacteria liquid cylinder 210 is pressed down to produce an indentation, the rotating lever 230 is pushed, so that the injection assembly 200 can be rotated in the frame 100, thereby facilitating the grinding of the bacteria liquid tank. In addition, the damaged leaf tissue can also be pushed away, thereby ensuring the accommodation space of the bacteria liquid tank, so that the subsequent bacteria liquid can be injected into the bacteria liquid tank.

[0048] In order to facilitate the tight connection between the bacteria liquid cylinder 210 and the sliding hole 110 , in some embodiments, a frosted layer is provided on the outer surface of the bacteria liquid cylinder 210 .

[0049] Specifically, by simply rubbing the outer surface of the bacteria liquid cylinder 210 with sandpaper, a sanding layer is formed on the outer peripheral side of the bacteria liquid cylinder 210, so that the friction between the bacteria liquid cylinder 210 and the sliding hole 110 can be increased, which facilitates the close connection between the bacteria liquid cylinder 210 and the sliding hole 110.

[0050] In order to prevent the bacteria liquid cartridge 210 from being separated from the frame 100 from the lower end, in some embodiments, a clamping portion 215 is protruded outwardly from the outer peripheral surface of the bacteria liquid cartridge 210 , and the clamping portion 215 is movably clamped with the surface of the frame 100 .

[0051] Specifically, the clamping portion 215 is configured as a clamping ring, the bottom surface of which is movably in contact with the top end surface of the frame 100, so as to be limited by the top end surface of the frame 100, thereby preventing the bacterial liquid cylinder 210 from sliding off the frame 100 from the lower end of the sliding hole 110. The rotating lever 230 is connected to the outer peripheral side of the clamping portion 215.

[0052] Example 3

[0053] It is understandable that when the amount of bacterial liquid required for inoculation of the leaves is small, a syringe with a smaller volume needs to be selected. For example, the bacterial liquid cartridge 210 can select a syringe with a volume of 1 mL. At this time, due to the small structure, the processing accuracy of the structure in the bacterial liquid cartridge 210 of Example 2 is required to be high. In order to avoid this situation, this embodiment is similar to Example 2, and the difference is that, in this embodiment, the structure of the limiting portion 214 is removed, and when the clamping portion 215 is clamped with the surface of the frame 100, the distance between the end surface of the bacterial liquid cartridge 210 close to the support portion 120 and the surface of the support portion 120 is 0.05 mm to 0.08 mm.

[0054] In this way, the injection assembly 200 can be prevented from being over-pressed by controlling the distance between the leaf-poking portion 213 and the support portion 120 when the bacterial liquid cylinder 210 slides to the bottom, thereby preventing the cassava leaves from being poked by the injection assembly 200. For example, the distance between the leaf-poking portion 213 and the support portion 120 when the bacterial liquid cylinder 210 slides to the bottom is 0.06 mm.

[0055] In some embodiments, the poking leaf portion 213 is configured as a square ring with a width of 0.45 cm to 0.55 cm.

[0056] In this way, when a small syringe is replaced and the width of the liquid outlet end 212 is less than 0.45cm to 0.55cm, the leaf-poking portion 213 can be set as a square ring connected to the liquid outlet end 212, and the bottom end of the square ring is also serrated, and the width between the two ends of the leaf-poking portion 213 is 0.5cm. In this way, it is convenient to directly press on the cassava leaves to form a bacterial liquid tank with an injection area of ​​0.25cm².

[0057] Example 4

[0058] This embodiment is a further implementation of Embodiment 1, 2 or 3. It is understandable that multiple points need to be inoculated on an existing cassava leaf, but the existing syringe can only inoculate one bacterial liquid tank at a time. In order to inoculate multiple points at a time, in this embodiment, the sliding hole 110 and the injection assembly 200 can be set to at least two.

[0059] Specifically, the sliding holes 110 are arranged in parallel, and the distance between the two sliding holes 110 is set corresponding to the distance between the inoculation points, so that the two bacterial liquid cylinders 210 can be pressed at the same time, so that two points of inoculation can be performed on one leaf at the same time.

[0060] In order to facilitate pressing down the bacteria liquid cylinder 210 and the piston push head 220 at the same time, the cassava leaf pathogen inoculator 10 may also be provided with a card plate, which is movably connected to the top ends of multiple bacteria liquid cylinders 210 or the top ends of multiple piston push heads 220 through a groove body, so that by pressing down the card plate, multiple bacteria liquid cylinders 210 or multiple piston push heads 220 can be driven to be pressed down.

[0061] Example 5

[0062] This embodiment is a further implementation of Embodiment 1, 2, 3 or 4. It is understandable that the injection assembly 200 needs to be set up aseptically. In order to avoid contamination caused by contact between the injection assembly 200 and the desktop during placement, in this embodiment, an abutment placement piece is provided on the outer side of the bacterial liquid cylinder 210.

[0063] Specifically, the abutment member may be, but is not limited to, an abutment block, and the thickness of the abutment block is set accordingly to support and place the bacterial liquid cylinder 210 in the air. The abutment member is used to separate the injection assembly 200 from a surface such as a table during the inoculation interval to prevent the surface such as the table from contaminating the injection assembly 200 and avoid the need for additional disinfection.

[0064] In the present invention, unless otherwise clearly specified and limited, the terms "install", "connect", "connect", "fix" and the like should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0065] In the description of the present utility model, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inside", "outside", etc. indicate the orientation or position relationship based on the orientation or position relationship shown in the accompanying drawings, or the orientation or position relationship in which the utility model product is usually placed when in use, which is only for the convenience of describing the utility model and simplifying the description, and does not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present utility model. In addition, the terms "first", "second", "third", etc. are only used to distinguish the description, and cannot be understood as indicating or implying relative importance.

[0066] In addition, the terms "horizontal", "vertical", "overhanging" and the like do not mean that the components are required to be absolutely horizontal or overhanging, but can be slightly tilted. For example, "horizontal" only means that its direction is more horizontal than "vertical", and does not mean that the structure must be completely horizontal, but can be slightly tilted.

[0067] In the present utility model, unless otherwise clearly specified and limited, the first feature being above or below the second feature may include the first and second features being in direct contact, or may include the first and second features not being in direct contact but being in contact through another feature between them. Moreover, the first feature being above, above, and above the second feature includes the first feature being directly above and obliquely above the second feature, or simply means that the first feature is higher in level than the second feature. The first feature being below, below, and below the second feature includes the first feature being directly below and obliquely below the second feature, or simply means that the first feature is lower in level than the second feature.

[0068] Although the utility model is described in conjunction with the above specific embodiments, it is obvious that those skilled in the art can make many substitutions, modifications and changes based on the above content. Therefore, all such substitutions, improvements and changes are included in the spirit and scope of the appended claims.

Claims

1. A cassava leaf pathogen inoculator, characterized in that: include: A frame (100) and an injection assembly (200); The frame (100) is configured as a concave structure, a sliding hole (110) is provided on one extending end of the frame (100), a support portion (120) for supporting the surface of a blade is provided on the surface of the other extending end of the frame (100), and the sliding hole (110) and the support portion (120) are arranged opposite to each other; The injection assembly (200) comprises a bacteria liquid cylinder (210) and a piston push head (220), wherein the piston push head (220) is slidably disposed in the bacteria liquid cylinder (210), a rotating lever (230) is disposed on the bacteria liquid cylinder (210), and an outer peripheral surface of the bacteria liquid cylinder (210) is tightly connected to a side wall of the sliding hole (110) so as to be relatively stationary under normal conditions, and under the action of an external force, the bacteria liquid cylinder (210) slides in the sliding hole toward the direction approaching the support portion (120), so that a bacteria liquid groove appears on the surface of the blade.

2. The cassava leaf pathogen inoculator according to claim 1, characterized in that: The bacterial liquid cylinder (210) comprises a metering end (211) and a liquid outlet end (212), and a leaf-poking portion (213) is provided on an end surface of the liquid outlet end (212) away from the metering end (211).

3. The cassava leaf pathogen inoculator according to claim 2, characterized in that: The number of the poking leaf parts (213) is set to be at least two.

4. The cassava leaf pathogen inoculator according to claim 1, characterized in that: The bacterial liquid cylinder (210) is provided with a limiting portion (214) for preventing the bacteria from penetrating the leaves.

5. The cassava leaf pathogen inoculator according to claim 4, characterized in that: The limiting portion (214) is configured as a silicone sheet.

6. The cassava leaf pathogen inoculator according to claim 1, characterized in that: The outer peripheral surface of the bacterial liquid cylinder (210) is provided with a clamping portion (215) protruding outward, and the clamping portion (215) is movably clamped with the surface of the frame (100).

7. The cassava leaf pathogen inoculator according to claim 6, characterized in that: When the engaging portion (215) is engaged with the surface of the frame (100), the distance between the end surface of the bacterial liquid cylinder (210) close to the supporting portion (120) and the surface of the supporting portion (120) is 0.05 mm to 0.08 mm.

8. The cassava leaf pathogen inoculator according to any one of claims 1 to 7, characterized in that: A frosted layer is provided on the outer surface of the bacterial liquid cylinder (210).

9. The cassava leaf pathogen inoculator according to any one of claims 1 to 7, characterized in that: The sliding hole (110) and the injection assembly (200) may be provided in at least two.

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