Protein prefabricated glue preparation device

Abstract

The utility model provides a protein prefabricated glue preparation device, and belongs to the technical field of biology. The protein prefabricated glue preparation device provided by the utility model comprises a glue plate main body and a glue plate comb, a containing cavity is formed in the rubber plate body, and a plug-in opening is formed in one end of the containing cavity. The rubber plate comb is inserted into the insertion port along the length direction of the rubber plate main body, and at least part of the rubber plate comb is accommodated in the accommodating cavity, so that gel in the accommodating cavity can form a gel hole; a guide part extending in the length direction is arranged on the rubber plate comb; a matching part corresponding to the guide part is arranged in the insertion port, and the matching part can be in sliding fit with the guide part in the length direction so as to limit the degree of freedom in the width direction when the rubber plate comb is inserted and pulled out. According to the protein prefabricated gel preparation device provided by the invention, the gel holes can be prevented from being damaged when the gel plate comb is pulled out, so that the analysis efficiency of gel electrophoresis is promoted to be improved.

Description

Technical Field

[0001] The present application relates to the field of biotechnology, and in particular to a device for preparing protein precast gels. Background Art

[0002] Gel electrophoresis is an important analytical method for separating and identifying biomacromolecules (such as proteins, peptides, etc.). During gel electrophoresis, a protein precast gel preparation device containing gel is placed in an electrophoresis tank, and protein samples are added to the gel holes of the protein precast gel preparation device. The protein samples are directed to move in the gel under the action of the electric field to achieve separation of the protein samples.

[0003] In the prior art, a protein precast gel preparation device is usually composed of a gel plate body and a gel plate comb. The gel plate body is designed with upper and lower openings, the upper opening is connected to the lower opening and forms a receiving cavity for accommodating the gel, and the gel plate comb is inserted into the receiving cavity so that the gel can wrap around the comb teeth of the gel plate comb and form gel holes.

[0004] When in use, the gel plate comb is pulled out from the gel plate body, and then the sample is added to the gel well. However, the pulling out process of the gel plate comb is very likely to produce movement in different directions, resulting in the destruction of the gel well structure, thereby reducing the analysis efficiency of gel electrophoresis.

[0005] In view of the above, there is an urgent need for a new protein precast gel preparation device to solve or at least alleviate the shortcomings of the above-mentioned prior art. Utility Model Content

[0006] The present application provides a protein precast gel preparation device, aiming to solve the problem in the prior art that the removal process of the gel plate comb of the protein precast gel preparation device easily damages the gel pore structure, resulting in low analysis efficiency of gel electrophoresis.

[0007] The present application provides a device for preparing protein precast gel, comprising a gel plate body and a gel plate comb; a receiving cavity is arranged in the gel plate body, and a plug-in interface is opened at one end of the receiving cavity; the gel plate comb is plugged into the plug-in interface along the length direction of the gel plate body, and is at least partially received in the receiving cavity, so that gel holes can be formed in the gel in the receiving cavity; a guide portion extending along the length direction is arranged on the gel plate comb; a matching portion corresponding to the guide portion is arranged in the plug-in interface, and the matching portion is arranged to be able to slide and match with the guide portion along the length direction, so as to limit the freedom of the gel plate comb in the width direction when the gel plate comb is inserted and removed.

[0008] In some embodiments, the rubber sheet comb includes a comb body and a plurality of comb teeth; the plurality of comb teeth are connected to one end of the comb body facing the plug-in interface and are arranged at intervals along the width direction; the comb teeth are inserted into the receiving cavity, and the two sides of the comb teeth along the thickness direction are tightly fitted with the rubber sheet body, and the cross-sectional size of the comb teeth in the thickness direction gradually decreases toward the receiving cavity at least partially; the guide portion is arranged on the comb body.

[0009] In some embodiments, the comb teeth include an extension section, a transition section and a protruding section connected in sequence along the length direction; one end of the extension section is connected to the comb body, and the other end extends toward the length direction; the transition section is provided with a first wedge surface and a second wedge surface relatively facing the rubber plate body, and the distance between the first wedge surface and the second wedge surface gradually decreases in the length direction; the protruding section extends along the length direction based on the transition section.

[0010] In some embodiments, the rubber plate body includes a first plate member and a second plate member; the first plate member is covered on the second plate member, and a receiving cavity is formed between the first plate member and the second plate member; an open groove is provided at one end of the first plate member for accommodating the comb body; the second plate member and the open groove form an insertion interface, and the mating part is provided on the second plate member.

[0011] In some embodiments, a first transition surface adapted to the first wedge-shaped surface is provided on one side of the first plate member opposite to the second plate member; a second transition surface adapted to the second wedge-shaped surface is provided on one side of the second plate member opposite to the first plate member; the first transition surface and the second transition surface are both provided with a main body surface extending along the length direction at one end away from the opening groove; the two main body surfaces are relatively arc-shaped and gradually transitioned at one end away from the opening groove to form a through opening, which is connected to the receiving cavity for contacting the gel with the electrophoresis liquid.

[0012] In some embodiments, a plurality of reinforcing ribs arranged at intervals along the length direction are provided on a surface of the first plate and / or the second plate facing away from the accommodating cavity; a reinforcing portion extending along the width direction is also provided on the first plate, and the reinforcing portion is arranged at the bottom of the opening groove and protrudes from the first plate in a direction away from the second plate.

[0013] In some embodiments, a sealing portion is further provided at one end of the first plate member and the second plate member away from the opening groove, the through opening is provided on the sealing portion, and the sealing portion is used for sealing by adhesive tape.

[0014] In some embodiments, the height difference between the two ends of the first wedge surface in the length direction is set to 0.8-1.2 mm; the height difference between the two ends of the second wedge surface in the length direction is set to 0.2-0.3 mm; the size of the protruding section along the length direction is set to 0.8-1.2 mm, and the size of the protruding section along the thickness direction is set to 0.5-2 mm.

[0015] In some embodiments, a protrusion is provided at one end of the comb body away from the comb teeth; an insertion groove recessed along the thickness direction is also provided on one side of the comb body away from the guide portion, and the insertion groove is at least partially provided on the protrusion.

[0016] In some embodiments, the guide portion is configured as a groove extending in the length direction and recessed in the thickness direction; the matching portion is configured as a ridge extending in the length direction and protruding in the thickness direction.

[0017] Compared with the prior art, the protein precast gel preparation device provided in this application has at least the following beneficial effects:

[0018] The present application optimizes the structure of a protein precast gel preparation device, and the protein precast gel preparation device includes two major components: a gel plate body and a gel plate comb; wherein a receiving chamber is provided in the gel plate body, so that the gel can be received in the receiving chamber, and a plug-in interface is provided at one end of the receiving chamber for the gel plate comb to be plugged and fixed on the gel plate body; the gel plate comb is plugged into the plug-in interface along the length direction of the gel plate body and at least partially received in the receiving chamber, and the gel plate comb located in the receiving chamber can make the gel including this part of the gel plate comb to form a gel hole; a guide portion extending along the length direction is provided on the gel plate comb, and a matching portion corresponding to the guide portion is provided in the plug-in interface, and the matching portion can slide and match with the guide portion along the length direction, so that the gel plate comb is limited in freedom in the width direction when being plugged in and out, and the gel plate comb can only move in the length direction, so that the damage to the gel hole structure caused by abnormal movement in the width direction can be avoided when it is pulled out, thereby greatly improving the analysis efficiency of gel electrophoresis.

[0019] Other advantages and features of the protein precast gel preparation device provided by the utility model will be further described in the subsequent specific embodiments. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] In order to more clearly illustrate the specific implementation of the present application or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the specific implementation or the prior art description. Obviously, the drawings described below are some implementations of the present application. For those skilled in the art, other drawings can be obtained based on these drawings without creative work.

[0021] Figure 1 This is a schematic diagram of the overall structure of the protein precast gel preparation device provided in this application;

[0022] Figure 2 A schematic diagram of the overall structure of the rubber comb provided in this application;

[0023] Figure 3 A schematic diagram of the overall structure of the rubber plate body provided in this application;

[0024] Figure 4 An enlarged view of the local structure A of the rubber comb provided in this application;

[0025] Figure 5 An exploded view of the protein precast gel preparation device provided in this application;

[0026] Figure 6 A front view of the protein precast gel preparation device provided in the present application;

[0027] Figure 7 This is a BB cross-sectional view of the protein precast gel preparation device provided in this application;

[0028] Figure 8 An enlarged view of the local structure C of the protein precast gel preparation device provided in this application;

[0029] Fig. 9 This is a bottom view of the protein precast gel preparation device provided in the present application.

[0030] The reference numerals are as follows:

[0031] 10. Protein precast gel preparation device;

[0032] 100, rubber plate body; 110, first plate; 111, opening groove; 112, first transition surface; 113, reinforcement portion; 120, second plate; 121, matching portion; 122, second transition surface; 123, matching section; 101, receiving cavity; 102, plug-in interface; 103, main body surface; 104, through-hole; 105, reinforcement rib; 106, sealing portion;

[0033] 200, rubber comb; 210, comb body; 211, guide portion; 212, plug-in slot; 213, protrusion; 220, comb teeth; 221, extension section; 222, transition section; 2221, first wedge-shaped surface; 2222, second wedge-shaped surface; 223, protrusion section. DETAILED DESCRIPTION

[0034] In the description of the present application, it should be understood that if terms such as "center", "longitudinal", "lateral", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise", "axial", "radial", "circumferential" and the like appear to indicate the orientation or position relationship, unless otherwise specified, they are understood to be based on the orientation or position relationship shown in the drawings, which is only for the convenience of describing the present application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as limiting the present application.

[0035] In addition, if there is a feature defined as "first" or "second", it is only used for descriptive purposes and cannot be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Features defined as "first" or "second" may explicitly or implicitly include at least one of the defined features. If there is a description of "plurality", the general meaning is to include at least two, such as two, three, etc., unless otherwise clearly and specifically defined.

[0036] In this application, unless otherwise clearly specified and limited, the terms such as "installed", "connected", "connected", "fixed" and so on should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integrated connection; it can be a mechanical connection or an electrical connection, it can be a direct connection, or it can be an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements. For those skilled in the art, the specific meanings of the above terms in this application can be understood according to the specific circumstances.

[0037] In the description of this specification, if the terms "one embodiment", "some embodiments", "example", "specific example", or "some examples" appear, it means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present application. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described may be combined in any one or more embodiments or examples in a suitable manner. In addition, those skilled in the art may combine and combine the different embodiments or examples described in this specification and the features of the different embodiments or examples, unless they are contradictory.

[0038] It should be noted that the length direction in the present application refers to the direction of the plugging of the rubber sheet comb 200, the thickness direction refers to the thickness direction of the rubber sheet body 100, and the width direction refers to the vertical direction of the plane formed by the length direction and the thickness direction.

[0039] Based on the above, the general concept of the embodiment of the utility model is to provide a protein precast gel preparation device 10, which mainly includes two major components: a gel plate body 100 and a gel plate comb 200. By setting a receiving cavity 101 in the gel plate body 100, the gel can be received in the gel plate body 100. By providing a plug-in interface 102 at one end of the receiving cavity 101, the gel plate comb 200 can be plugged into the gel plate body 100 through the plug-in interface 102, and the gel plate comb 200 can be partially received in the receiving cavity 101, so that the gel plate comb 200 in the receiving cavity 101 can be wrapped after the receiving cavity 101 is filled with gel. 0, to form a gel hole; by providing a guide portion 211 extending along the length direction on the gel plate comb 200, and correspondingly providing a matching portion 121 on the plug-in interface 102 of the gel plate main body 100, the gel plate comb 200 can be plugged in and out of the gel plate main body 100 through the cooperation between the guide portion 211 and the corresponding matching portion 121, and the matching portion 121 limits the freedom of the guide portion 211 in the width direction, so that the gel plate comb 200 only moves in the plug-in direction (length direction), thereby avoiding damage to the gel holes due to abnormal movement in the width direction when the protein precast gel preparation device 10 is used, thereby promoting the improvement of the analysis efficiency of gel electrophoresis.

[0040] Based on the above general concept, reference Figures 1 to 9 As shown, an embodiment of the present application provides a protein precast gel preparation device 10, including a gel plate body 100 and a gel plate comb 200; a receiving cavity 101 is provided in the gel plate body 100, and an insertion port 102 is opened at one end of the receiving cavity 101; the gel plate comb 200 is plugged into the insertion port 102 along the length direction of the gel plate body 100, and is at least partially received in the receiving cavity 101, so that gel holes can be formed in the gel in the receiving cavity 101; the gel plate comb 200 is provided with a guide portion 211 extending along the length direction; a matching portion 121 corresponding to the guide portion is provided in the insertion port 102, and the matching portion 121 is configured to be able to slide and cooperate with the guide portion 211 along the length direction, so as to limit the freedom of the gel plate comb 200 in the width direction when plugging and unplugging.

[0041] It can be understood that in this embodiment, the gel can be placed in the receiving cavity 101 through the plug interface 102. After the gel fills the receiving cavity 101, the gel comb 200 passes through the plug interface 102 along the length direction of the gel body 100 and is partially inserted into the receiving cavity 101, so that the gel in the receiving cavity 101 is squeezed by the gel comb 200 to form gel holes, so that when the protein precast gel preparation device 10 is used, the gel can be loaded through the gel holes by pulling out the gel comb 200.

[0042] Therefore, through the design of the guide part 211 on the rubber sheet comb 200 and the matching part 121 on the rubber sheet main body 100, the rubber sheet comb 200 is limited in the width direction of the matching part 121 and in the thickness direction of the rubber sheet main body 100, so that the part of the rubber sheet comb 200 located in the receiving cavity 101 can be stably pulled out along the length direction, thereby ensuring the stability of the rubber hole.

[0043] Specifically, refer to Figure 2 and Figure 3 As shown, the guide portion 211 in this embodiment is configured as a groove extending along the length direction and recessed along the thickness direction; and the matching portion 121 is configured as a ridge extending along the length direction and protruding along the thickness direction. The ridge and the groove are adapted to each other so that the rubber plate comb 200 can be stably pulled out of the rubber hole during use to avoid damage to the rubber hole.

[0044] It should be clarified that the guide portion 211 in some embodiments can also be designed as a ridge extending in the length direction and protruding in the thickness direction, and the matching portion 121 can also be correspondingly designed as a groove extending in the length direction and recessed in the thickness direction, that is, the guide portion 211 and the matching portion 121 only need to satisfy the guidance in the length direction and the limitation in the width direction.

[0045] In addition, in this embodiment, two guide portions 211 are arranged at intervals along the width direction, and correspondingly, two matching portions 121 are also arranged at intervals along the width direction. Through the design of two guide portions 211 and two matching portions 121, the abnormal movement of the rubber plate comb in the width direction when plugging and unplugging can be further avoided, so as to significantly improve the structural stability of the rubber hole; in some implementations, the number of guide portions 211 and matching portions 121 can also be designed to be more than two, that is, to ensure that the guide portion 211 can be slidably matched with the corresponding matching portion 121 in the length direction.

[0046] Continue to refer Figure 2 In order to further improve the stability of the glue hole, the rubber plate comb 200 in this embodiment includes a comb body 210 and a plurality of comb teeth 220; the plurality of comb teeth 220 are connected to one end of the comb body 210 facing the plug-in port 102 and are arranged at intervals along the width direction; the comb teeth 220 are inserted into the receiving cavity 101, and the two sides of the comb teeth 220 along the thickness direction are tightly fitted with the rubber plate body 100, and the cross-sectional size of the comb teeth 220 in the thickness direction is gradually reduced toward the plug-in port 102 at least partially; two guide portions 211 are arranged on the comb body 210 at intervals along the width direction.

[0047] The comb body 210 in this embodiment is a plate-shaped member, and a plurality of comb teeth 220 are connected to one end of the comb body 210 facing the plug-in port 102 and are arranged at intervals along the width direction. After the rubber sheet comb 200 is inserted into the rubber sheet body 100, the comb teeth 220 are inserted into the receiving cavity 101, and the two surfaces of the comb teeth 220 along the thickness direction are tightly fitted with the rubber sheet body 100, so that the degree of freedom of the comb teeth 220 in the thickness direction is limited by the rubber sheet body 100, and the two guide portions 211 are arranged at intervals along the width direction on the comb body 210. 0, thereby limiting the freedom of the comb teeth 220 in the width direction, so that the comb teeth 220 can only be pulled out along the length direction; after the gel is filled in the receiving cavity 101, multiple comb teeth 220 are inserted into the gel, and the gel is filled in the interval space between the multiple comb teeth 220 to form a glue hole. The design of the cross-sectional size of the comb teeth 220 in the thickness direction gradually shrinking at least partially toward the receiving cavity 101 can avoid scratching the glue hole when the comb teeth 220 are pulled out, so that the stability of the glue hole is further improved.

[0048] For further information, please refer to Figure 4 In order to facilitate the loading of samples into the gel holes formed by the gel, the comb teeth 220 include an extension section 221, a transition section 222 and a protruding section 223 connected in sequence along the length direction; one end of the extension section 221 is connected to the comb body 210, and the other end extends toward the length direction; the transition section 222 is provided with a first wedge surface 2221 and a second wedge surface 2222 facing the gel plate body 100, and the distance between the first wedge surface 2221 and the second wedge surface 2222 gradually decreases in the length direction; the protruding section 223 extends along the length direction based on the transition section 222.

[0049] In this embodiment, the extension section 221 is in the shape of a square column as a whole. One end of the extension section 221 is connected to the comb body 210, and the other end extends in the length direction and connects to the transition section 222, so that the top section of the glue hole can be in the shape of a square column; the transition section 222 is a double-sided wedge-shaped column, and the two sides of the transition section 222 facing the rubber plate body 100 are respectively a first wedge surface 2221 and a second wedge surface 2222, and the distance between the first wedge surface 2221 and the second wedge surface 2222 gradually decreases in the length direction, so that the center of the glue hole The section is wide at the top and narrow at the bottom, so that when adding samples to the gel well, a sample adding mechanism such as a pipette or a dropper has more operating space, which is convenient for loading samples into the gel well and significantly increases the sample holding capacity of the gel well; the protruding section 223 is square columnar as a whole, and its thickness is less than that of the extending section 221, so that the bottom section of the gel well is also square columnar, providing a more regular swimming lane for the sample, allowing the sample to swim more evenly in the bottom section of the gel well in a direction away from the plug interface 102 under the action of the electric field force.

[0050] Furthermore, refer to Figure 5The rubber plate body 100 in this embodiment includes a first plate member 110 and a second plate member 120; the first plate member 110 is covered on the second plate member 120, and a receiving cavity 101 is formed between the first plate member 110 and the second plate member 120; an opening groove 111 is provided at one end of the first plate member 110 for accommodating the comb body 210; the second plate member 120 and the opening groove 111 form an insertion interface 102, and two matching parts 121 are provided on the second plate member 120.

[0051] In this embodiment, the rubber plate body 100 mainly includes a first plate member 110 and a second plate member 120, wherein the first plate member 110 is covered on the second plate member 120, and a receiving cavity 101 is formed between the first plate member 110 and the second plate member 120 to accommodate the comb teeth 220 and the gel; wherein, an opening groove 111 facing the thickness direction is further provided at one end of the first plate member 110, and the opening groove 111 and the second plate member 120 jointly form a plug-in interface 102, so that a side of the comb body 210 facing away from the second plate member 120 can be exposed by the opening groove 111, so as to increase the contact area of ​​the rubber plate comb 200 when plugging and unplugging; two matching parts 121 are provided on the second plate member 120, and a side of the comb body 210 facing away from the first plate member 110 is in contact with the second plate member 120, and the guide part 211 of the comb body 210 can slide along the matching part 121, thereby preventing the comb teeth 220 from twisting the glue hole and causing damage to the glue hole.

[0052] Considering the wedge-shaped structure of the comb teeth 220, reference Figures 5 to 9 In this embodiment, a first transition surface 112 adapted to the first wedge surface 2221 is provided on one side of the first plate 110 opposite to the second plate 120; a second transition surface 122 adapted to the second wedge surface 2222 is provided on one side of the second plate 120 opposite to the first plate 110; the first transition surface 112 and the second transition surface 122 are both provided with a main body surface 103 extending along the length direction at one end away from the opening groove 111; the two main body surfaces 103 are relatively arc-shaped and gradually transitioned at one end away from the opening groove 111 to form a through hole 104, and the through hole 104 is connected to the receiving chamber 101 for contacting the gel with the electrophoresis liquid.

[0053] It can be understood that, since the spacing between the first wedge-shaped surface 2221 and the second wedge-shaped surface 2222 on the comb teeth 220 gradually decreases in the direction toward the receiving cavity 101, the spacing between the first transition surface 112 and the second transition surface 122 in the direction away from the plug-in port 102 in this embodiment is gradually reduced in adaptability, so as to respectively fit with the first wedge-shaped surface 2221 and the second wedge-shaped surface 2222. The ends of the first transition surface 112 and the second transition surface 122 away from the opening groove 111 are both designed to be formed by a main body surface 103 extending in the length direction, and the spacing between the two main body surfaces 103 is adapted to the thickness of the protruding section 223, so that the protruding section 223 can fit closely with the two main body surfaces 103 in the thickness direction after being inserted into the receiving cavity 101.

[0054] In addition, in order to meet the power supply requirement of the gel, a through hole 104 is provided at one end of the receiving cavity 101 away from the plug interface 102 in the present embodiment, so that the gel can contact the electrophoresis liquid through the through hole 104 during electrophoresis; the through hole 104 is formed by a relatively arc-shaped tapered transition at one end of the two main body surfaces 103 away from the opening groove 111. The relatively arc-shaped tapered transition at one end of the two main body surfaces 103 away from the opening groove 111 can reduce the distance between the two main body surfaces 103 away from the end of the opening groove 111, thereby reducing the size of the formed through hole 104 in the thickness direction, so that the bottom of the receiving cavity 101 is in a rounded-corner closing form. Through this design, the binding force of the first plate 110 and the second plate 120 on the gel can be enhanced, so that the gel can be stably fixed in the receiving cavity 101, and the gel can be prevented from overflowing from the through hole 104 at the bottom of the rubber plate body 100, and at the same time, the expansion phenomenon caused by the gel contacting with the electrophoresis liquid during electrophoresis is improved.

[0055] like Fig. 9 As shown, in order to facilitate the sealing of the internal gel during storage of the protein precast gel preparation device 10, a sealing portion 106 is further provided at one end of the first plate 110 and the second plate 120 away from the opening groove 111 in this embodiment, and the through-hole 104 is provided on the sealing portion 106, and the sealing portion 106 is used for sealing with tape.

[0056] It can be understood that the sealing portion 106 can be used for tape to stick, and the through-hole 104 is arranged on the sealing portion 106. Since the through-hole 104 is formed by a relatively arc-shaped gradual transition at one end of the two main surfaces 103 away from the opening groove 111, the through-hole 104 is smaller in thickness direction than conventional designs, so that the tape has a larger sticking area, the bonding strength of the tape is improved, and the sealing of the through-hole 104 is improved, thereby avoiding the expansion phenomenon caused by contact with water when the gel is stored.

[0057] Continue to refer Figure 5 and Figure 6 In order to improve the deformation resistance of the rubber plate body 100, the first plate 110 and the second plate 120 in this embodiment are both provided with a plurality of reinforcing ribs 105 arranged at intervals along the length direction on the side facing away from the receiving cavity 101. Among them, two reinforcing ribs 105 are designed on the first plate 110, and three reinforcing ribs 105 are designed on the second plate 120. The reinforcing ribs 105 on the first plate 110 and the reinforcing ribs 105 on the second plate 120 are aligned in the thickness direction, so that the overall deformation resistance of the rubber plate body 100 is improved, so as to avoid deformation caused by the internal gel, resulting in bending or irregularity of the experimental strip (gel as a whole) after electrophoresis; at the same time, through this design, the rubber plate body 100 can be prevented from being touched by the sample loading mechanism during the sample loading process, resulting in the separation of the first plate 110 and the second plate 120, and the phenomenon of sample leakage or sample string holes.

[0058] In addition, since the sample loading mechanism needs to load samples to the glue holes through the opening groove 111, in order to avoid structural damage to the opening groove 111 of the first plate 110 when the sample loading mechanism loads samples, a reinforcement portion 113 is also designed on the first plate 110 in this embodiment. The reinforcement portion 113 extends along the width direction and is located at the bottom of the opening groove 111. By designing the reinforcement portion 113 to protrude from the first plate 110 in a direction away from the second plate 120, the thickness of the bottom of the opening groove 111 is increased to enhance the load resistance of the bottom of the opening groove 111, so that when the opening groove 111 of the first plate 110 is subjected to a load applied by the sample loading mechanism, structural deformation and damage are not easily generated. In this embodiment, the height difference between the two ends of the first wedge-shaped surface 2221 in the length direction is set to 0.8-1.2 mm; the height difference between the two ends of the second wedge-shaped surface 2222 in the length direction is set to 0.2-0.3 mm; thus, the first wedge-shaped surface 2221 has a higher slope in the length direction than the second wedge-shaped surface 2222, so as to provide a higher operating space for the sample loading mechanism, and make the glue hole have a higher volume, so as to facilitate the increase of the sample loading amount; and the size of the protruding section 223 along the length direction is set to 0.8-1.2 mm to ensure that the bottom section of the glue hole has a certain depth of hole depth, so that the sample can be subjected to a more uniform electric field force at the bottom section of the glue hole, and the size of the protruding section 223 along the thickness direction is set to 0.5-2 mm, so that the gel thickness between the two main surfaces 103 can meet common requirements.

[0059] In a specific embodiment, the number of comb teeth 220 is designed to be 15, so that 15 glue holes can be formed in the receiving cavity 101, and the height difference between the two ends of the first wedge surface 2221 in the length direction is set to 1mm; the height difference between the two ends of the second wedge surface 2222 in the length direction is set to 0.25mm; the thickness of the protruding section 223 is designed to be 1mm, so that the thickness of most of the gel except for the part close to the plug-in port 102 and the through-port 104 is 1mm.

[0060] It should be noted that the number of the comb teeth 220 in this embodiment can be adaptively designed according to the requirements, and the thickness of the protruding section 223 can be adjusted according to the requirements of the gel thickness.

[0061] like Figure 6As shown, in order to facilitate the plugging and unplugging of the rubber sheet comb 200, a protrusion 213 is provided at one end of the comb body 210 away from the comb teeth 220 in this embodiment, and the protrusion 213 protrudes from the comb body 210 in a direction away from the comb teeth 220. A plugging and unplugging groove 212 recessed along the thickness direction is also provided on the side of the comb body 210 away from the guide portion 211, and the plugging and unplugging groove 212 is at least partially provided on the protrusion 213. The plugging and unplugging groove 212 can be exposed to the rubber sheet body along the thickness direction through the opening groove 111, so that the operator can plug and unplug the rubber sheet comb 200 on the rubber sheet body through the plugging and unplugging groove 212 on the protrusion 213, so as to improve the plugging and unplugging convenience of the rubber sheet comb 200.

[0062] It should be noted that in this embodiment, two protrusions 213 are arranged at intervals along the width direction, so that the operator can hold it with both hands, further improving the convenience of plugging and unplugging the rubber comb 200. In addition, in order to increase the contact area between the second plate 120 and the rubber comb 200, the second plate 120 is also designed with a matching section 123 with the same shape as the protrusion 213 projected along the thickness direction. The matching section 123 is designed at the end of the second plate 120 away from the through-hole 104, so that after the rubber comb 200 is plugged into the rubber body 100, the protrusion 213 can fit tightly with the matching section 123, avoiding the rubber comb 200 from being touched by external objects and causing abnormal movement in the thickness direction, thereby damaging the first plate 110, the second plate 120 or the glue hole and other structures, so as to improve the structural stability of the protein precast gel preparation device 10.

[0063] In summary, the embodiment of the present application optimizes the structure of the protein precast gel preparation device 10 so that the freedom of the gel comb 200 in the width direction is restricted when the gel comb 200 is plugged in and out, and the gel comb 200 can only move in the length direction, thereby avoiding damage to the gel hole structure due to twisting and turning in the width direction when being pulled out; by further structural design of the comb body 210, comb teeth 220, the first plate 110 and the second plate 120, the overall strength of the gel body 100 is higher, the operating space of the sample loading mechanism is larger during sample loading, sample loading is more convenient, and the sample loading amount is larger, thereby synergistically promoting the improvement of the analysis efficiency of gel electrophoresis.

[0064] Although the embodiments of the present application have been shown and described above, it can be understood that the above embodiments are exemplary and cannot be understood as limitations on the present application. Those skilled in the art can change, modify, replace and modify the above embodiments within the scope of the present application.

Claims

1. A protein precast gel preparation device (10), characterized in that: It comprises a rubber sheet body (100) and a rubber sheet comb (200); A receiving cavity (101) is provided in the rubber plate body (100), and an insertion port (102) is provided at one end of the receiving cavity (101); The rubber sheet comb (200) is inserted into the insertion port (102) along the length direction of the rubber sheet body (100) and is at least partially received in the receiving cavity (101), so that the gel in the receiving cavity (101) can form gel holes; The rubber plate comb (200) is provided with a guide portion (211) extending along the length direction; A matching portion (121) corresponding to the guide portion is arranged in the plug-in port (102); the matching portion (121) is arranged to be able to slide and match with the guide portion (211) along the length direction, so as to limit the degree of freedom of the rubber comb (200) in the width direction when plugging and unplugging.

2. The protein precast gel preparation device (10) according to claim 1, characterized in that: The rubber comb (200) comprises a comb body (210) and a plurality of comb teeth (220); The plurality of comb teeth (220) are all connected to one end of the comb body (210) facing the plugging port (102), and are arranged at intervals along the width direction; The comb teeth (220) are inserted into the receiving cavity (101), and the two surfaces of the comb teeth (220) along the thickness direction are tightly fitted with the rubber plate body (100), and the cross-sectional dimensions of the comb teeth (220) in the thickness direction at least partially gradually decrease toward the receiving cavity (101); The guide portion (211) is arranged on the comb body (210).

3. The protein precast gel preparation device (10) according to claim 2, characterized in that: The comb teeth (220) include an extension section (221), a transition section (222) and a protruding section (223) which are connected in sequence along the length direction; One end of the extension section (221) is connected to the comb body (210), and the other end extends toward the length direction; The transition section (222) is provided with a first wedge-shaped surface (2221) and a second wedge-shaped surface (2222) facing the rubber plate body (100) relatively, and the distance between the first wedge-shaped surface (2221) and the second wedge-shaped surface (2222) gradually decreases in the length direction; The protruding section (223) extends along the length direction based on the transition section (222).

4. The protein precast gel preparation device (10) according to claim 3, characterized in that: The rubber plate body (100) comprises a first plate component (110) and a second plate component (120); The first plate (110) is covered on the second plate (120), and the receiving cavity (101) is surrounded by the first plate (110) and the second plate (120); An open groove (111) is provided at one end of the first plate (110) for accommodating the comb body (210); The second plate (120) and the opening groove (111) form the plug-in port (102), and the matching portion (121) is arranged on the second plate (120).

5. The protein precast gel preparation device (10) according to claim 4, characterized in that: A first transition surface (112) adapted to the first wedge surface (2221) is provided on a surface of the first plate (110) opposite to the second plate (120); A second transition surface (122) adapted to the second wedge surface (2222) is provided on a surface of the second plate (120) opposite to the first plate (110); The first transition surface (112) and the second transition surface (122) are both provided with a main body surface (103) extending along the length direction at one end away from the opening groove (111); One end of the two main body surfaces (103) away from the opening groove (111) is relatively arc-shaped and gradually shrinks to form a through hole (104), and the through hole (104) is connected to the receiving cavity (101) to allow the gel to contact the electrophoresis liquid.

6. The protein precast gel preparation device (10) according to claim 4, characterized in that: A plurality of reinforcing ribs (105) arranged at intervals along the length direction are provided on a surface of the first plate member (110) and / or the second plate member (120) facing away from the receiving cavity (101); The first plate member (110) is also provided with a reinforcing portion (113) extending in the width direction; the reinforcing portion (113) is arranged at the bottom of the opening groove (111) and protrudes from the first plate member (110) in a direction away from the second plate member (120).

7. The protein precast gel preparation device (10) according to claim 5, characterized in that: A sealing portion (106) is also provided at one end of the first plate member (110) and the second plate member (120) away from the opening groove (111), the through opening (104) is provided on the sealing portion (106), and the sealing portion (106) is used for sealing with adhesive tape.

8. The protein precast gel preparation device (10) according to claim 3, characterized in that: The height difference between the two ends of the first wedge-shaped surface (2221) in the length direction is set to 0.8-1.2 mm; The height difference between the two ends of the second wedge-shaped surface (2222) in the length direction is set to 0.2-0.3 mm; The dimension of the protruding section (223) along the length direction is set to 0.8-1.2 mm, and the dimension of the protruding section (223) along the thickness direction is set to 0.5-2 mm.

9. The protein precast gel preparation device (10) according to claim 2, characterized in that: A protrusion (213) is provided at one end of the comb body (210) away from the comb teeth (220); A plug-in groove (212) recessed in the thickness direction is further provided on one side of the comb body (210) away from the guide portion (211); the plug-in groove (212) is at least partially provided on the protruding portion (213).

10. The protein precast gel preparation device (10) according to any one of claims 1 to 9, characterized in that: The guide portion (211) is configured as a groove extending along the length direction and recessed along the thickness direction; The matching portion (121) is configured as a convex ridge extending along the length direction and protruding along the thickness direction.

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