Biological ultralow-temperature vacuum liquid nitrogen box

By using vacuum interlayer technology and duplex design of sample test tubes in biological ultra-low temperature vacuum liquid nitrogen box, the problem of difficulty in protecting the originality and integrity of biological samples in the existing technology in ultra-low temperature operation is solved, and a more realistic and more Efficient data acquisition and sample storage.

CN222442888UActive Publication Date: 2025-02-11HEFEI KEYOUEN BIOELECTRONICS TECH CO LTD
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Patent Information

Application Number
CN202421088932.1
Authority / Receiving Office
CN · China
Patent Type
Utility models(China)
Current Assignee / Owner
Filing Date
2024-05-20
Publication Date
2025-02-11
Estimated Expiration
2034-05-20

AI Technical Summary

Technical Problem

The existing ultra-low temperature liquid nitrogen cartridges are difficult to effectively protect the originality and integrity of biological samples during operation. Especially when dealing with low abundance and easily degradable samples, the advantages of low temperature operation are not obvious, and there is cross-contamination of samples. risk.

Method used

A biological ultra-low temperature vacuum liquid nitrogen box was designed, using vacuum interlayer technology to isolate the air conditioner to prevent frost and condensation on the peripheral surface. A double station of the sample test tube was set up on the cover plate table to achieve the simultaneous adoption of multiple samples to prevent cross-contamination of samples.

Benefits of technology

Through the entire low-temperature operation, the originality and integrity of biological samples are protected without destroying sample activity, and more, better and more realistic data are obtained, especially for low-abundance and easy-to-degradation samples, preventing cross-contamination of samples.

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Abstract

The utility model discloses a biological ultra-low temperature vacuum liquid nitrogen box which comprises a heat insulation base, a vacuum outer system, a vacuum inner shell system, a waste material pipe, a cover plate table and a liquid nitrogen inlet and outlet, the bottom of the vacuum outer system is fixedly installed on the top of the heat insulation base, the vacuum inner shell system is fixedly installed in the vacuum outer system, and the waste material pipe is connected with the cover plate table. And the waste material pipe is fixedly mounted on one side of the vacuum external system. The whole design operation process of the ultralow-temperature liquid nitrogen box is carried out under a low-temperature condition, so that the primitiveness and the integrity of a biological sample are protected to the greatest extent, the activity of the biological sample is not damaged, more, better and more real data can be obtained, and the ultralow-temperature liquid nitrogen box is particularly suitable for some low-abundance and easily-degradable samples. Low-temperature operation has very obvious advantages, a low-temperature environment in a sampling process is kept, and a sample is not damaged; the vacuum interlayer technology isolates cold air and prevents the periphery from frosting and condensing; the sample test tube is provided with double stations, multiple samples are collected simultaneously, and cross contamination of the samples is prevented.
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Description

Technical Field

[0001] The utility model relates to the technical field of liquid nitrogen boxes, in particular to a biological ultra-low temperature vacuum liquid nitrogen box. Background Art

[0002] The biological ultra-low temperature vacuum liquid nitrogen box is a special device used for long-term low-temperature storage of biological samples. Its working principle is based on the ultra-low temperature characteristics of liquid nitrogen (-196°C). This type of liquid nitrogen container uses advanced vacuum insulation technology, which can greatly slow down the evaporation rate of liquid nitrogen, thereby effectively preserving samples such as cells, tissues, DNA, embryos, sperm, eggs and other biological materials at ultra-low temperatures without inactivation.

[0003] The existing ultra-low temperature liquid nitrogen boxes are designed to be operated under low temperature conditions throughout the entire process to prevent damage to the originality and integrity of biological samples, destroy the activity of biological samples, and affect more realistic data. Low temperature operation has very obvious advantages for some low-abundance and easily degradable samples. A low temperature environment is required to maintain the sampling process, otherwise it will affect the experimental results.

[0004] Therefore, how to provide a biological ultra-low temperature vacuum liquid nitrogen box is a problem that technicians in this field urgently need to solve. Utility Model Content

[0005] One purpose of the utility model is to provide a biological ultra-low temperature vacuum liquid nitrogen box. The design and operation of the ultra-low temperature liquid nitrogen box of the utility model are carried out under low temperature conditions throughout the whole process, which protects the originality and integrity of biological samples to the greatest extent, will not destroy the activity of biological samples, and can obtain more, better and more real data. Especially for some low-abundance and easily degradable samples, low-temperature operation has very obvious advantages, maintaining a low-temperature environment during the sampling process, and will not damage the samples; vacuum interlayer technology isolates cold air and prevents frost and condensation on the periphery; sample test tubes have double stations, and multiple samples are taken at the same time; and cross contamination of samples is prevented.

[0006] A biological ultra-low temperature vacuum liquid nitrogen box according to an embodiment of the utility model comprises an insulating base, a vacuum outer system, a vacuum inner shell system, a waste pipe, a cover plate platform and a liquid nitrogen inlet and outlet, wherein the bottom of the vacuum outer system is fixedly mounted on the top of the insulating base, the vacuum inner shell system is fixedly mounted in the vacuum outer system, the waste pipe is fixedly mounted on one side of the vacuum outer system, the liquid nitrogen inlet and outlet are fixedly mounted on the other side of the vacuum outer system, and the cover plate platform is fixedly mounted on the top of the vacuum outer system.

[0007] Furthermore, the top of the waste pipe extends out of the cover plate platform.

[0008] Furthermore, the top of the liquid nitrogen inlet and outlet extends out of the cover plate platform.

[0009] The beneficial effects of the utility model are:

[0010] The design and operation of the ultra-low temperature liquid nitrogen box of the utility model are carried out under low temperature conditions throughout the whole process, which protects the originality and integrity of biological samples to the greatest extent, will not destroy the activity of biological samples, and can obtain more, better and more real data. Especially for some low-abundance and easily degradable samples, low-temperature operation has very obvious advantages, maintaining a low-temperature environment during the sampling process, and will not damage the samples; vacuum interlayer technology isolates cold air and prevents frost and condensation on the periphery; the sample test tube has double stations, and multiple samples are taken at the same time to prevent cross contamination of samples. BRIEF DESCRIPTION OF THE DRAWINGS

[0011] The accompanying drawings are used to provide a further understanding of the present invention and constitute a part of the specification. Together with the embodiments of the present invention, they are used to explain the present invention and do not constitute a limitation of the present invention. In the accompanying drawings:

[0012] Figure 1 The utility model provides a schematic diagram of the overall planar structure of a biological ultra-low temperature vacuum liquid nitrogen box.

[0013] In the figure: 1. Insulation base; 2. Vacuum outer system; 3. Vacuum inner shell system; 4. Waste pipe; 5. Cover plate platform; 6. Liquid nitrogen inlet and outlet. DETAILED DESCRIPTION

[0014] Now the utility model is further described in detail with reference to the accompanying drawings. These drawings are simplified schematic diagrams, which only illustrate the basic structure of the utility model in a schematic manner, and therefore only show the components related to the utility model.

[0015] A biological ultra-low temperature vacuum liquid nitrogen box refers to a liquid nitrogen container specially designed for storing biological samples in an ultra-low temperature environment (usually -80℃-100℃). The interior of this container often has a sophisticated structure to ensure the safe storage and convenient operation of the samples. The design and operation of the ultra-low temperature liquid nitrogen box are carried out under low temperature conditions throughout the process, which protects the originality and integrity of the biological samples to the greatest extent, will not destroy the activity of the biological samples, and can obtain more, better and more real data. Especially for some low-abundance and easily degradable samples, low-temperature operation has a very obvious advantage, maintaining a low temperature environment during the sampling process and will not damage the samples.

[0016] Please refer to Figure 1The utility model provides a biological ultra-low temperature vacuum liquid nitrogen box, comprising an insulating base 1, a vacuum outer system 2, a vacuum inner shell system 3, a waste pipe 4, a cover plate platform 5 and a liquid nitrogen inlet and outlet 6, wherein the bottom of the vacuum outer system 2 is fixedly installed on the top of the insulating base 1, the insulating base 1 is used for heat insulation, the vacuum outer system 2 is used for the outer shell, the vacuum inner shell system 3 is fixedly installed in the vacuum outer system 2, the vacuum inner shell system 3 is used for placing samples, a vacuum interval is formed between the vacuum outer system 2 and the vacuum inner shell system 3, the vacuum interlayer technology isolates the cold air, and prevents the periphery of the liquid nitrogen box from frosting and condensing, the waste pipe 4 is fixedly installed on the side of the vacuum outer system 2, the waste pipe 4 is used to discharge the gasified liquid nitrogen, the liquid nitrogen inlet and outlet 6 is fixedly installed on the other side of the vacuum outer system 2, the liquid nitrogen inlet and outlet 6 is used for the entry and outflow of liquid nitrogen, the cover plate platform 5 is fixedly installed on the top of the vacuum outer system 2, the cover plate platform 5 is used to cover the vacuum outer system 2 and the vacuum inner shell system 3 to form a closed space, and a double station for sample test tubes is arranged on the cover plate platform 5, and multiple samples are taken at the same time to prevent cross contamination of samples.

[0017] The top of the waste pipe 4 extends out of the cover plate platform 5, which is convenient for discharging vaporized nitrogen. Liquid nitrogen will evaporate rapidly at room temperature and atmospheric pressure to produce a large amount of nitrogen. The waste pipe connects the inside and outside of the liquid nitrogen box to discharge the nitrogen generated by continuous evaporation to maintain the stability of the pressure in the liquid nitrogen box and prevent safety hazards caused by excessive pressure; the top of the liquid nitrogen inlet and outlet 6 extends out of the cover plate platform 5, and is mainly used to add liquid nitrogen to the liquid nitrogen box to maintain the liquid nitrogen storage volume in the container, ensuring that the biological sample is always immersed in liquid nitrogen to achieve an extremely low temperature freezing preservation effect; when the liquid nitrogen in the container is consumed less due to natural evaporation, new liquid nitrogen can be safely injected through the liquid nitrogen inlet to keep the inside of the container in a stable ultra-low temperature environment.

[0018] By placing the collected biological samples in a test tube and placing them in a stable ultra-low temperature environment through liquid nitrogen, we can avoid damaging the originality and integrity of the biological samples, destroying the activity of the biological samples, and affecting more realistic data. Low-temperature operation has very obvious advantages for some low-abundance and easily degradable samples. A low-temperature environment is required to maintain the sampling process, otherwise it will affect the experimental results.

[0019] The design and operation of the biological ultra-low temperature vacuum liquid nitrogen box are carried out under low temperature conditions throughout the process, which protects the originality and integrity of biological samples to the greatest extent, will not destroy the activity of biological samples, and can obtain more, better and more real data. Especially for some low-abundance and easily degradable samples, low-temperature operation has very obvious advantages. Maintaining a low-temperature environment during the sampling process will not damage the sample; vacuum interlayer technology isolates cold air and prevents frost and condensation on the periphery; sample tubes have double stations, and multiple samples are taken at the same time to prevent cross-contamination of samples.

[0020] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes within the technical scope disclosed by the present invention according to the technical scheme and the utility model concept of the present invention, which should be covered by the protection scope of the present invention.

Claims

1. Biological ultra-low temperature vacuum liquid nitrogen box, characterized in that: The invention comprises a heat-insulating base (1), a vacuum outer system (2), a vacuum inner shell system (3), a waste pipe (4), a cover plate platform (5) and a liquid nitrogen inlet and outlet (6), wherein the bottom of the vacuum outer system (2) is fixedly mounted on the top of the heat-insulating base (1), the vacuum inner shell system (3) is fixedly mounted in the vacuum outer system (2), the waste pipe (4) is fixedly mounted on one side of the vacuum outer system (2), the liquid nitrogen inlet and outlet (6) is fixedly mounted on the other side of the vacuum outer system (2), and the cover plate platform (5) is fixedly mounted on the top of the vacuum outer system (2).

2. A biological ultra-low temperature vacuum liquid nitrogen box according to claim 1, characterized in that: The top of the waste pipe (4) extends out of the cover plate platform (5).

3. A biological ultra-low temperature vacuum liquid nitrogen box according to claim 1, characterized in that: The top of the liquid nitrogen inlet and outlet (6) extends out of the cover plate platform (5).