Stirrer for polypeptide synthesis

Abstract

A stirring mechanism comprises a stirring motor, the output end of the stirring motor is connected with a stirring shaft, the stirring shaft is fixedly provided with a stirring column, the bottom end of the stirring shaft is provided with a mounting groove, the interior of the mounting groove is detachably connected with a scraping mechanism, and the stirring shaft rotating at a high speed generates a siphonic effect in a cylindrical barrel. Polypeptide raw materials synthesized in the barrel are sucked into the through holes to be collided, dispersed and mixed, and are diffused and thrown out through centrifugal force of high-speed rotation of the stirring column, so that a good material mixing effect is achieved, a lifting motor is matched for synchronous use, and the height of the stirring column in the barrel is adjusted through the lifting motor, so that materials with different heights are synchronously mixed; the problem of layering of the materials in the barrel can be avoided, and the lifting stirring mechanism can be conveniently and directly taken out for cleaning.

Description

Technical Field

[0001] The utility model belongs to the technical field of polypeptide synthesis, and in particular relates to a stirrer used for polypeptide synthesis. Background Art

[0002] A polypeptide is a compound formed by amino acids linked together by peptide bonds. It is also an intermediate product of protein hydrolysis. A compound formed by the dehydration condensation of two amino acid molecules is called a dipeptide. Similarly, there are tripeptides, tetrapeptides, pentapeptides, etc. Usually, compounds formed by the dehydration condensation of three or more amino acid molecules can be called polypeptides. Therefore, in modern medicine, polypeptides need to be synthesized to facilitate the research on antibodies in medicine. During the polypeptide synthesis process, a stirrer is needed to stir and mix the polypeptide raw materials.

[0003] At present, most of the existing agitators used for peptide synthesis are installed in one piece with a stirring tank. After use, it is not convenient to directly take out the agitator for cleaning. The staff can only roughly clean the agitator by flushing water into the tank. The agitator has the problem of not being cleaned thoroughly, and it is difficult to quickly clean the stirring shaft after the stirring is completed. At the same time, it is impossible to fully stir materials at different heights, and the stirring effect is poor. Utility Model Content

[0004] The utility model aims to provide a stirrer for polypeptide synthesis, so as to solve the problems of rapid cleaning of the stirring shaft and adjustment of the stirring height in the prior art.

[0005] In order to achieve the above purpose, the utility model adopts the following technical solutions:

[0006] A stirrer for peptide synthesis, comprising a support plate, a placing plate fixed to the bottom of the support plate, a controller installed on the outside of the support plate, a fixed plate fixed to the top of the support plate, a top plate fixed to the top of the fixed plate, a lifting motor fixedly connected to the top of the top plate, a screw rod installed at the output end of the lifting motor, a slide bar symmetrically arranged below the top plate, a lifting plate slidably installed on the slide bar, a middle part of one end of the lifting plate meshes with the screw rod, and a stirring mechanism installed on the lifting plate;

[0007] The stirring mechanism comprises a stirring motor, the output end of the stirring motor is connected to a stirring shaft, a stirring column is fixed on the stirring shaft, a mounting groove is provided at the bottom end of the stirring shaft, and a scraping mechanism is detachably connected in the mounting groove;

[0008] A sliding disc is slidably connected to the stirring shaft, and a cleaning mechanism is connected to the sliding disc for cleaning the stirring column.

[0009] Furthermore, the surface of the stirring column is penetrated with a plurality of through holes. The polypeptide raw materials synthesized in the barrel are sucked into the through holes, collided, dispersed and mixed, and then diffused and thrown out by the centrifugal force of the high-speed rotation of the stirring column, thereby achieving a good material mixing effect.

[0010] Furthermore, the scraping mechanism includes a connecting plate, one end of which is provided with a circular joint, and the other end of which is provided with a scraper. A threaded column is fixed at the center of the installation groove, and cutouts are symmetrically opened on both sides of the installation groove. A threaded sleeve is threadedly engaged and installed on the threaded column.

[0011] Furthermore, a reinforcing rib is provided between the connecting plate and the scraper, and a plurality of circular holes are penetrated on the surface of the reinforcing rib. When the stirring shaft rotates, the polypeptide raw material will pass through the circular holes to help diffuse the polypeptide raw material inside.

[0012] Furthermore, the cleaning mechanism includes cleaning rollers and corner rods, the cleaning rollers are equidistantly distributed below the sliding plate, the number of the cleaning rollers corresponds to the number of through holes, one end of the corner rod is fixed to one side of the sliding plate, the other end of the corner rod passes through the lifting plate, an adsorption plate is fixed to the top of the other end of the corner rod, and both ends of the adsorption plate are slidably mounted on the sliding rod.

[0013] Furthermore, the adsorption plate is made of magnetic material. The magnetic material is made of ferromagnetic or NdFeB magnets and other materials with strong magnetic force. When the adsorption plate moves to the top, the adsorption plate will be firmly adsorbed under the top plate to prevent the adsorption plate from automatically sliding down and affecting the work of the stirring column.

[0014] Furthermore, a brush layer is distributed on the periphery of the cleaning roller. Under the action of the brush layer, the cleaning roller can well clean the materials on the inner wall of the through hole.

[0015] The technical solution of the utility model has the following beneficial effects:

[0016] 1. The high-speed rotating stirring shaft produces a siphon effect in the cylindrical barrel. The polypeptide raw materials synthesized in the barrel are sucked into the through hole for collision, dispersion and mixing. The high-speed rotating centrifugal force of the stirring column diffuses and throws it out, thereby achieving a good material mixing effect. It is used synchronously with the lifting motor. The height of the stirring column in the barrel is adjusted by the lifting motor, so that materials at different heights can be mixed synchronously. This can avoid the problem of stratification of materials in the barrel. The lifting stirring mechanism can be conveniently taken out and cleaned directly.

[0017] 2. The adsorption plate is pulled down by force, and the corner rod fixes the adsorption plate and the sliding plate together. The displacement of the adsorption plate drives the sliding plate to move synchronously. The material attached to the inner wall of the through hole is cleaned by pulling the cleaning roller back and forth. Under the action of the brush layer, the cleaning roller can well sweep the material on the inner wall of the through hole, and then rinse with water. The cleaning mechanism can clean the stirring column simply and quickly. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] In order to more clearly illustrate the technical solutions of the embodiments of the present utility model, the drawings required for describing the embodiments are briefly introduced below.

[0019] Figure 1 It is a schematic diagram of the overall structure of the utility model.

[0020] Figure 2 It is a schematic diagram of the bottom structure of the utility model.

[0021] Figure 3 This is a schematic diagram of the positioning interval structure of the utility model.

[0022] Figure 4 This is a structural schematic diagram of embodiment 4 of the present utility model.

[0023] Attached figures: 10, support plate; 11, placement plate; 12, controller; 13, fixed plate; 14, top plate; 15, lifting motor; 16, slide rod; 17, lifting plate; 18, screw; 19, stirring motor; 20, stirring shaft; 21, stirring column; 22, through hole; 23, mounting groove; 24, incision; 25, connecting plate; 26, circular joint; 27, threaded sleeve; 28, threaded column; 29, scraper; 30, reinforcing rib; 31, sliding plate; 32, cleaning roller; 33, corner rod; 34, adsorption plate; 35, handle. DETAILED DESCRIPTION

[0024] In order to make the purpose, technical solution and advantages of the utility model clearer, the utility model will be further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the utility model and are not used to limit the utility model. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the utility model.

[0025] Embodiment 1:

[0026] refer to Figure 1-Figure 4 A stirrer for peptide synthesis comprises a support plate 10, a placing plate 11 is fixed at the bottom of the support plate 10, a cylindrical barrel is placed on the placing plate 11, a peptide is added inside the cylindrical barrel, a controller 12 is installed on the outside of the support plate 10, a fixing plate 13 is fixed above the support plate 10, a top plate 14 is fixed on the top of the fixing plate 13, a lifting motor 15 is fixedly connected above the top plate 14, a screw 18 is installed at the output end of the lifting motor 15, a sliding rod 16 is symmetrically arranged below the top plate 14, a lifting plate 17 is slidably installed on the sliding rod 16, and a stirring mechanism is installed on the lifting plate 17;

[0027] In the above scheme, a cylindrical barrel is placed on the placing plate 11, and polypeptide raw materials are added inside the cylindrical barrel. The symmetrically arranged sliding rods 16 stabilize the position of the lifting plate 17. The controller 12 controls the start of the lifting motor 15. The middle part of one end of the lifting plate 17 is meshed with the screw 18. The rotation of the screw 18 can drive the lifting plate 17 to move up and down, thereby driving the stirring mechanism to move up and down. The position of the stirring mechanism is changed to achieve the purpose of mixing materials at different heights.

[0028] Furthermore, the stirring mechanism includes a stirring motor 19, the output end of the stirring motor 19 is connected to a stirring shaft 20, and a stirring column 21 is fixed on the stirring shaft 20; the controller 12 controls the stirring motor 19 to start, the stirring shaft 20 will rotate at a high speed, and a plurality of through holes 22 are penetrated through the surface of the stirring column 21. The high-speed rotating stirring shaft 20 produces a siphon effect in the cylindrical barrel, and the polypeptide raw materials synthesized in the barrel are sucked into the through holes 22 for collision, dispersion and mixing, and are diffused and thrown out by the centrifugal force of the high-speed rotation of the stirring column 21, thereby achieving a good material mixing effect. The lifting motor 15 is used synchronously to adjust the height of the stirring column 21 in the barrel by the lifting motor 15, so as to synchronously mix materials at different heights, which can avoid the problem of stratification of materials in the barrel, and the lifting stirring mechanism can be conveniently taken out and cleaned directly.

[0029] It is worth noting that the stirring motor 19 and the lifting motor 15 are electrically connected to the controller 12 , and are uniformly controlled to be turned on and off by the controller 12 .

[0030] Embodiment 2:

[0031] A mounting groove 23 is provided at the bottom end of the stirring shaft 20, and a scraping mechanism is detachably connected in the mounting groove 23. The scraping mechanism includes a connecting plate 25, one end of the connecting plate 25 is provided with a circular joint 26, and the other end of the connecting plate 25 is provided with a scraper 29. A threaded column 28 is fixed at the center of the mounting groove 23, and cutouts 24 are symmetrically opened on both sides of the mounting groove 23. A threaded sleeve 27 is threadedly engaged with the threaded column 28.

[0032] In the above scheme, after the materials in the barrel are stirred and mixed by the stirring mechanism, the lifting motor 15 is started by the controller 12 to move the lifting plate 17 to the top, and then the circular joint 26 is sleeved on the threaded column 28, and the connecting plate 25 is limited in the cutout 24 to prevent the problem of deviation when the stirring shaft 20 rotates. Finally, the threaded sleeve 27 is threadedly engaged and installed on the threaded column 28, so that the two connecting plates 25 are fixed inside the installation groove 23. After the installation, the two connecting plates 25 are arranged at 180 degrees, and the scraper 29 is attached to the inner wall of the barrel. The stirring motor 19 is started by the controller 12, and the scraper 29 scrapes the raw materials remaining on the inner wall of the barrel. With the use of the lifting motor 15, when performing the scraping operation, the displacement of the lifting plate 17 changes the height of the scraper 29, thereby achieving all-round scraping of the inner wall of the barrel.

[0033] It is worth noting that: the length of the connecting plate 25 determines the radius formed by the scraper 29, and the connecting plate 25 that is adapted to each other is matched according to the size of the barrel. When the scraping operation is performed by the scraping mechanism, the speed of the stirring motor 19 needs to be adjusted by the controller 12, and the raw materials remaining on the inner wall of the barrel can be scraped off by slow speed; after the stirring column 21 is stirred at a high speed, the scraper 29 can also be used as a stirring function by slow speed to further mix the polypeptide raw materials.

[0034] Furthermore, a reinforcing rib 30 is provided between the connecting plate 25 and the scraper 29, and the reinforcing rib 30 is used to strengthen the connection strength between the connecting plate 25 and the scraper 29 to prevent the connection plate 25 and the scraper 29 from being deformed due to resistance during stirring. A plurality of circular holes are penetrated on the surface of the reinforcing rib 30, and when the stirring shaft 20 rotates, the polypeptide raw material will pass through the circular holes to help diffuse the polypeptide raw material inside.

[0035] Embodiment three:

[0036] A sliding plate 31 is slidably connected to the stirring shaft 20, and a cleaning mechanism is connected to the sliding plate 31 for cleaning the stirring column 21. The cleaning mechanism includes cleaning rollers 32 and corner rods 33. The cleaning rollers 32 are equidistantly distributed below the sliding plate 31. One end of the corner rod 33 is fixed to one side of the sliding plate 31, and the other end of the corner rod 33 passes through the lifting plate 17. An adsorption plate 34 is fixed to the top of the other end of the corner rod 33. Both ends of the adsorption plate 34 are slidably sleeved on the sliding rod 16, and a handle 35 is provided on the outer side of the adsorption plate 34.

[0037] In the above scheme, the number of cleaning rollers 32 corresponds to the number of through holes 22, and the adsorption plate 34 is limited to move on the slide bar 16. When the inside of the through hole 22 needs to be cleaned by the cleaning roller 32, it is only necessary to pull down the adsorption plate 34 with force, and the corner rod 33 fixes the adsorption plate 34 and the sliding plate 31. The displacement of the adsorption plate 34 synchronously drives the sliding plate 31 to move, and the cleaning roller 32 is pulled back and forth to clean the material attached to the inner wall of the through hole 22. When cleaning by the cleaning roller 32, each through hole 22 needs to be vertically aligned with each cleaning roller 32. A brush layer is distributed on the periphery of the cleaning roller 32. Under the action of the brush layer, the cleaning roller 32 can well sweep the material on the inner wall of the through hole 22, and then rinse with water. The cleaning mechanism can simply and quickly clean the stirring column 21.

[0038] It is worth noting that the rotation of the stirring shaft 20 will not drive the sliding plate 31 to rotate.

[0039] Furthermore, the top plate 14 is made of metal material, and the adsorption plate 34 is made of magnetic material. The magnetic material is made of a material with strong magnetic force such as ferromagnetism or neodymium iron boron magnets. When the adsorption plate 34 moves to the top, the adsorption plate 34 will be firmly adsorbed under the top plate 14 to prevent the adsorption plate 34 from automatically sliding down and affecting the operation of the stirring column 21.

[0040] The specific implementation process of this embodiment is as follows:

[0041] When in use, the controller 12 controls the stirring motor 19 to start, the stirring shaft 20 will rotate at a high speed, and a plurality of through holes 22 are penetrated on the surface of the stirring column 21. The high-speed rotating stirring shaft 20 produces a siphon effect in the cylindrical barrel, and the polypeptide raw materials synthesized in the barrel are sucked into the through holes 22 for collision, dispersion and mixing, and then diffused and thrown out by the centrifugal force of the high-speed rotation of the stirring column 21, thereby achieving a good material mixing effect. The lifting motor 15 is used synchronously, and the height of the stirring column 21 in the barrel is adjusted by the lifting motor 15, so that materials at different heights can be mixed synchronously;

[0042] During cleaning, when it is necessary to clean the inside of the through hole 22 with the cleaning roller 32, it is only necessary to pull down the adsorption plate 34 with force, and the corner rod 33 fixes the adsorption plate 34 and the sliding plate 31 together. The adsorption plate 34 produces displacement and simultaneously drives the sliding plate 31 to move. The cleaning roller 32 is pulled back and forth to clean the material attached to the inner wall of the through hole 22. Under the action of the brush layer, the cleaning roller 32 can well sweep the material on the inner wall of the through hole 22, and then rinse with water.

[0043] The above embodiments are only exemplary embodiments of the present invention and are not intended to limit the present invention. The protection scope of the present invention is defined by the claims. Various modifications or equivalent substitutions may be made to the present invention within the essence and protection scope of the present invention. Such modifications or equivalent substitutions shall also be deemed to fall within the protection scope of the present invention.

[0044] In the description of the present utility model, it should be noted that the terms "inside", "front", "back", "left", "right" and the like indicate directions or positional relationships based on the directions or positional relationships shown in the attached circles, or the directions or positional relationships in which the utility model product is usually placed when in use, and are only used to facilitate the description of the present utility model and simplify the description, rather than indicating or implying that the device or element referred to must have a specific direction, be constructed and operated in a specific direction. Therefore, these terms indicating directions or positional relationships cannot be understood as limiting the present utility model.

[0045] In the description of the present invention, it is further explained that, unless otherwise clearly specified and limited, the terms "disposed" and "connected" should be understood in a broad sense. For example, these terms can indicate a fixed connection, a detachable connection, or an integral connection between elements; they can also indicate a mechanical connection, an electrical connection; they can also indicate a direct connection or an indirect connection through an intermediate medium. For ordinary technicians in this field, the specific meanings of these terms in the present invention can be understood according to specific circumstances.

Claims

1. A stirrer for peptide synthesis, comprising a support plate (10), a placing plate (11) is fixed at the bottom of the support plate (10), a controller (12) is installed on the outside of the support plate (10), a fixing plate (13) is fixed above the support plate (10), and a top plate (14) is fixed on the top of the fixing plate (13), characterized in that: A lifting motor (15) is fixedly connected above the top plate (14), a screw rod (18) is installed at the output end of the lifting motor (15), a sliding rod (16) is symmetrically provided below the top plate (14), a lifting plate (17) is slidably installed on the sliding rod (16), a middle part of one end of the lifting plate (17) is meshed with the screw rod (18), and a stirring mechanism is installed on the lifting plate (17); The stirring mechanism comprises a stirring motor (19), the output end of the stirring motor (19) is connected to a stirring shaft (20), a stirring column (21) is fixed on the stirring shaft (20), a mounting groove (23) is provided at the bottom end of the stirring shaft (20), and a scraping mechanism is detachably connected in the mounting groove (23); A sliding disk (31) is slidably connected to the stirring shaft (20), and a cleaning mechanism is connected to the sliding disk (31) for cleaning the stirring column (21).

2. A stirrer for polypeptide synthesis according to claim 1, characterized in that: A plurality of through holes (22) penetrate the surface of the stirring column (21).

3. A stirrer for polypeptide synthesis according to claim 2, characterized in that: The scraping mechanism comprises a connecting plate (25), one end of which is provided with a circular joint (26), the other end of which is provided with a scraper (29), a threaded column (28) is fixed at the center of the installation groove (23), notches (24) are symmetrically opened on both sides of the installation groove (23), and a threaded sleeve (27) is threadedly engaged and installed on the threaded column (28).

4. A stirrer for polypeptide synthesis according to claim 3, characterized in that: A reinforcing rib (30) is provided between the connecting plate (25) and the scraper (29), and a plurality of circular holes penetrate through the surface of the reinforcing rib (30).

5. A stirrer for polypeptide synthesis according to claim 2, characterized in that: The cleaning mechanism comprises cleaning rollers (32) and corner rods (33), wherein the cleaning rollers (32) are equidistantly distributed below the sliding plate (31), the number of the cleaning rollers (32) corresponds to the number of the through holes (22), one end of the corner rod (33) is fixed to one side of the sliding plate (31), the other end of the corner rod (33) passes through the lifting plate (17), an adsorption plate (34) is fixed to the top of the other end of the corner rod (33), and the two ends of the adsorption plate (34) are slidably sleeved on the sliding rod (16).

6. A stirrer for polypeptide synthesis according to claim 5, characterized in that: The adsorption plate (34) is made of magnetic material.

7. A stirrer for polypeptide synthesis according to claim 6, characterized in that: A brush layer is distributed on the periphery of the cleaning roller (32).

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