Combined nucleic acid pretreatment equipment

Abstract

The utility model provides combined nucleic acid pretreatment equipment, which comprises a sampling and detecting tube rack and can improve the convenience of a user in operation. The sampling and detecting pipe frame comprises an upper-layer plate, a lower-layer plate and four supports, the upper-layer plate comprises a plurality of first through holes and a plurality of upper-layer guides, the upper-layer guides are arranged in the first through holes of the upper-layer plate, and the inner wall of each upper-layer guide is provided with a slope. The lower layer plate is parallel to the upper layer plate and comprises a plurality of second through holes and a plurality of lower layer guides, and one of the second through holes corresponds to one of the first through holes. Each lower-layer guide is provided with a peripheral wall and an inclined plane, and the inclined plane is arranged at the top end of the peripheral wall and contracts inwards from the peripheral wall to the interior and the axis direction of the lower-layer guide. And the four brackets are respectively arranged between the upper-layer plate and the lower-layer plate.

Description

Technical Field

[0001] The utility model relates to a combined nucleic acid pre-treatment device, in particular to a combined nucleic acid pre-treatment device comprising a sampling and inspection tube rack and a nucleic acid extraction hole plate transmission module. Background Art

[0002] In the prior art nucleic acid pretreatment equipment, the user often needs to manually operate the barcode scanning gun to scan the sample tube and then place the sample tube back into the sample tube rack. The user must pay attention to the placement and direction of the sample tube rack and carefully place the sample tube so that it can be completely placed in the sample tube rack, which causes inconvenience to the user in operation.

[0003] In addition, when the user is transporting the carrier plate with the nucleic acid extraction well plate, the user must pay attention to the placement and direction of the carrier plate to avoid danger in subsequent processes when the carrier plate is placed in the wrong position.

[0004] Therefore, the nucleic acid pretreatment equipment in the prior art causes the user to spend too much time on processes such as confirming whether the placement position and direction of the sample tube rack are correct, manually scanning the sample tube, and whether the placement position and direction of the carrier are correct.

[0005] Therefore, it is an urgent problem to be solved in the art to develop a combined nucleic acid pretreatment device that can assist in determining whether the placement and orientation of the sample tube rack are correct, automatically scan the sample tubes, and confirm whether the position of the carrier is correct, so as to improve the user's convenience in operation. Utility Model Content

[0006] In order to solve the above-mentioned problems of the prior art, the purpose of the utility model is to provide a combined nucleic acid pretreatment device that can assist in confirming whether the placement direction of the sample tube rack is correct, automatically scan the sample tubes, and confirm whether the position of the carrier is correct, so as to achieve the purpose of improving the user's convenience in operation.

[0007] In order to achieve the above-mentioned object, the utility model provides a combined nucleic acid pretreatment device, including a sampling and inspection tube rack, the sampling and inspection tube rack includes:

[0008] An upper plate, comprising a plurality of first through holes and a plurality of upper guides, each of which is an annular hollow structure and is respectively disposed in the first through hole of the upper plate; an inner wall of each upper guide has an inclined surface;

[0009] A lower plate is parallel to the upper plate, and includes a plurality of second through holes and a plurality of lower guides, one of the plurality of second through holes corresponds to one of the plurality of first through holes; each lower guide is respectively disposed in the second through hole of each lower plate, and each lower guide has a peripheral wall and an inclined surface, and the peripheral wall surrounds to form an accommodation space; the inclined surface is disposed at the top of the peripheral wall and shrinks from the peripheral wall toward the inside and the axial direction of the lower guide; and

[0010] Four brackets are respectively arranged between the upper plate and the lower plate, one end of each bracket is respectively fixed to one of the four corners of the lower plate, and the other end of each bracket is respectively fixed to one of the four corners of the upper plate.

[0011] In a specific embodiment, the lower plate further includes a first positioning pin hole and a second positioning pin hole, the first positioning pin hole is arranged at one corner of the lower plate, and the second positioning pin hole is arranged at another corner of the lower plate. The inner diameter of the first positioning pin hole is different from the inner diameter of the second positioning pin hole. The arrangement of the first positioning pin hole and the second positioning pin hole can assist the positioning of the sampling inspection tube rack on the sampling inspection tube loading and unloading platform, and avoid the placement direction error of the sampling inspection tube when it is placed on the sampling inspection tube loading and unloading platform.

[0012] In a specific embodiment, the first positioning pin hole and the second positioning pin hole are arranged diagonally.

[0013] In a specific embodiment, the height H1 of the peripheral wall of the lower guide is greater than 8.3 mm, each lower guide has a guide angle height greater than 4 mm, and each lower guide has a guide angle θ ranging between 36° and 49.9°. When the bottom of the sampling inspection tube contacts the inclined surface of the upper guide, the sampling inspection tube can smoothly slide into the hollow structure of the upper guide, so that the bottom of the sampling inspection tube is located in the accommodation space of the lower guide.

[0014] In a specific embodiment, the nucleic acid pretreatment device further includes a nucleic acid extraction disk transfer module, and the nucleic acid extraction disk transfer module is arranged at a height lower than the sampling and inspection tube rack. The nucleic acid extraction disk transfer module includes:

[0015] Two tracks are disposed oppositely at two sides of the nucleic acid extraction disk conveying module and extend from a first end to an opposite second end of the nucleic acid extraction disk conveying module respectively;

[0016] Two conveyor belts are respectively arranged above the track;

[0017] a tray elastic support member, disposed at the first end of the nucleic acid extraction tray conveying module, and

[0018] A carrier plate positioning detection member is disposed at the first end of the nucleic acid extraction plate transmission module and is adjacent to the carrier plate elastic positioning member.

[0019] In a specific embodiment, the acid extraction disk transfer module further includes a tray positioning stopper, and the tray positioning stopper is disposed at the second end of the nucleic acid extraction disk transfer module.

[0020] In a specific embodiment, the nucleic acid extraction plate transfer module further includes two carrier plate lifting safety detection parts, which are respectively arranged on one side of the nucleic acid extraction plate transfer module and adjacent to the track, and on the other side of the nucleic acid extraction plate transfer module and adjacent to the track.

[0021] In a specific embodiment, the nucleic acid extraction plate transfer module further includes a plate lifting assembly, which is disposed between two tracks of the nucleic acid extraction plate transfer module, the plate elastic support member and the plate in-position stopper.

[0022] In a specific embodiment, the nucleic acid extraction disk transfer module further includes at least two carrier disk positioning pins, and the at least two carrier disk positioning pins are respectively disposed diagonally at the first end and the second end of the nucleic acid extraction disk transfer module.

[0023] In a specific embodiment, the nucleic acid extraction disk transfer module also includes a first carrier in place detection component and at least one second carrier in place detection component, the first carrier in place detection component is arranged at the second end of the nucleic acid extraction disk transfer module, and the at least one second carrier in place detection component is respectively arranged on the carrier lifting assembly and the second end of the nucleic acid extraction disk transfer module.

[0024] The nucleic acid extraction disk transfer module of the present invention has the following advantages: (1) the first positioning pin hole and the second positioning pin hole of the sample test tube rack are provided to assist the sample test tube rack in positioning on the sample test tube loading and unloading platform, thereby preventing the sample test tube from being placed in the wrong direction when it is placed on the sample test tube loading and unloading platform; (2) the upper guide and the lower guide of the sample test tube rack can make the sample test tube slide smoothly into the accommodating space of the lower guide; (3) the multiple second through holes in the lower layer of the sample test tube rack can simultaneously affix two through holes to the tube body during the automated process of transferring the position of the sample test tube or opening and closing the tube cover of the sample test tube. (4) The carrier elastic support member can assist the user in confirming that the carrier has been stably placed in the position of the nucleic acid extraction disk transfer module, and the carrier support detection member can check whether the carrier is indeed in the correct position; (5) The carrier lifting safety detection member, the carrier support detection member and the first carrier in place detection member can detect whether the carrier is in the correct position to avoid the danger of the carrier being in the wrong position or state during the subsequent process; and (6) The second carrier in place detection member can check the progress of the carrier during the conveying process. In summary, the nucleic acid extraction disk transfer module of the present invention can prevent the user from spending too much time to confirm whether the position of the carrier is correct, and can avoid time loss caused by the wrong position of the carrier, and avoid the danger of carrying out the subsequent process in the wrong state. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 It is a top view of the combined nucleic acid pretreatment device of the utility model.

[0026] Figure 2 It is a side view of the combined nucleic acid pretreatment device of the utility model.

[0027] Figure 3A It is a stereoscopic diagram of a sampling and testing tube rack of the combined nucleic acid pretreatment equipment of the utility model.

[0028] Figure 3B It is a stereoscopic diagram of a sampling and testing tube rack of the combined nucleic acid pretreatment equipment of the utility model.

[0029] Figure 3C It is a schematic diagram of the process of a sampling and inspection tube of the combined nucleic acid pretreatment equipment of the utility model falling into a sampling and inspection tube rack.

[0030] Figure 3D It is a schematic diagram of the sampling and inspection tube of the combined nucleic acid pretreatment equipment of the utility model being located on the sampling and inspection tube rack.

[0031] Figure 3E A top view of the lower plate of the combined nucleic acid pretreatment device of the present invention is shown.

[0032] Figure 4A A three-dimensional diagram showing the nucleic acid extraction well plate transfer module and the carrier plate of the combined nucleic acid pre-treatment device of the present invention.

[0033] Figure 4B A three-dimensional diagram showing the combination of the nucleic acid extraction well plate transfer module and the carrier plate of the combined nucleic acid pre-treatment device of the present invention.

[0034] Figure 4C It is a top view of a nucleic acid extraction well plate transfer module of the combined nucleic acid pretreatment device of the utility model.

[0035] Description of Reference Numerals

[0036] 10: loading and unloading platform for sampling and inspection tube, 222: first positioning pin hole,

[0037] 20: sampling and inspection tube rack, 223: second positioning pin hole,

[0038] 21: upper plate, 23: bracket,

[0039] 211: upper guide, 30: nucleic acid extraction plate transfer module,

[0040] 2111: slope, 31: track,

[0041] 22: lower plate, 32: conveyor belt,

[0042] 221: lower layer guide, 33: carrier elastic support member,

[0043] 2211: inclined plane, 34: carrier plate position detection part,

[0044] 35: Carrier stopper,

[0045] 36: Carrier lifting safety detection parts,

[0046] 37: Carrier lifting assembly,

[0047] 38: Carrier positioning pin,

[0048] 39A: First tray in place detection part,

[0049] 39B: Second tray in place detection part,

[0050] 40: sampling tube,

[0051] 50: Carrier plate,

[0052] A1: working area,

[0053] H1: height,

[0054] H2: height,

[0055] θ: Guidance angle. DETAILED DESCRIPTION

[0056] The following describes the implementation of the present invention through specific embodiments. Those skilled in the art can understand other advantages and effects of the present invention through the contents disclosed in this specification. However, the exemplary embodiments disclosed in the present invention are for illustrative purposes only and should not be considered as limiting the scope of the present invention. In other words, the present invention can also be implemented or applied through other different specific embodiments, and the details in this specification can also be modified and changed in various ways based on different viewpoints and applications without departing from the spirit of the present invention.

[0057] As used in the specification and the appended claims, the singular forms "a," "an," and "the" include plural forms unless otherwise indicated herein.

[0058] Unless otherwise indicated herein, the term "or" used in the specification and the appended claims includes the meaning of "and / or".

[0059] The term "working area" used in the present invention refers to an area where automated processes such as position transfer of the sampling and inspection tube or opening and closing of the tube cover of the sampling and inspection tube are performed.

[0060] See also Figure 1 and Figure 2 The combined nucleic acid pretreatment device includes a sampling tube loading and unloading platform 10, three sampling tube racks 20 and a nucleic acid extraction disk transfer module 30, and the sampling tube racks 20 are arranged on the sampling tube loading and unloading platform 10. The sampling tube loading and unloading platform 10 is located in the working area A1 of the combined nucleic acid pretreatment device 1.

[0061] See also Figure 3A and Figure 3B The sampling and inspection pipe rack 20 includes an upper plate 21, a lower plate 22 and four brackets 23. Each bracket 23 is arranged between the upper plate 21 and the lower plate 22. One end of each bracket 23 is respectively fixed to one of the four corners of the lower plate 22, and the other end of each bracket 23 is respectively fixed to one of the four corners of the upper plate 21. The upper plate 21 is parallel to the lower plate 22.

[0062] See also FIG. 3B to FIG. 3E The upper plate 21 includes a plurality of first through holes and a plurality of upper guides 211. Each upper guide 211 is an annular hollow structure and is respectively disposed in the first through hole of the upper plate 21. The inner wall of each upper guide 211 has an inclined surface 2111.

[0063] The lower plate 22 includes a plurality of second through holes, a plurality of lower guides 221, a first positioning pin hole 222 and a second positioning pin hole 223, and one of the plurality of second through holes corresponds to one of the plurality of first through holes. Each lower guide 221 is respectively disposed in the second through hole of each lower plate 22, and each lower guide 221 has a peripheral wall and a slope 2211, and the peripheral wall surrounds to form a containing space; the height H1 of the peripheral wall of the lower guide 221 is 10.5 mm; the slope 2211 is disposed at the top of the peripheral wall and shrinks from the peripheral wall toward the inside and axial direction of the lower guide 221, so that each lower guide 221 has a guide angle height H2 of 4 mm; at this time, each lower guide 221 has a guide angle θ ranging between 36° and 49.9°. When the bottom of the sampling and inspection tube 40 contacts the inclined surface of the upper guide 211 , the sampling and inspection tube 40 can smoothly slide into the hollow structure of the upper guide 211 , so that the bottom of the sampling and inspection tube 40 is located in the accommodating space of the lower guide 221 .

[0064] The first positioning pin hole 222 is set at one corner of the lower plate 22, and the second positioning pin hole 223 is set at the other corner of the lower plate 22, and the second positioning pin hole 223 is set diagonally to the first positioning pin hole 222. The inner diameter of the second positioning pin hole 223 is smaller than the inner diameter of the first positioning pin hole 222. The first positioning pin hole 222 and the second positioning pin hole 223 can assist in positioning the sampling inspection tube rack 20 on the sampling inspection tube loading and unloading platform 10, and avoid the sampling inspection tube rack 20 being placed in the wrong direction when being placed on the sampling inspection tube loading and unloading platform 10.

[0065] See also Figure 1 , Figure 3D and Figure 3E In the working area A1, a two-dimensional barcode scanner is arranged below the sampling and inspection tube loading and unloading platform 10. Through the multiple second through holes of the lower plate 22 of the sampling and inspection tube rack 20, the sampling and inspection tube 40 with a two-dimensional barcode attached to the tube body can be scanned simultaneously during the automated processes such as the position transfer of the sampling and inspection tube 40 or the opening and closing of the tube cover of the sampling and inspection tube 40, thereby saving the time of the automated process.

[0066] See also Figure 1 , FIG. 4A to FIG. 4CThe nucleic acid extraction disk transfer module 30 is arranged at a height lower than the sampling and inspection tube rack 20 and adjacent to the inlet and outlet of the combined nucleic acid pretreatment equipment. The nucleic acid extraction disk transfer module 30 includes two tracks 31, two conveyor belts 32, a carrier elastic positioning member 33, a carrier positioning detection member 34, a carrier in place stopper 35, two carrier lifting safety detection members 36, a carrier lifting assembly 37, two carrier positioning pins 38, a first carrier in place detection member 39A, and two second carrier in place detection members 39B. Each track 31 is relatively arranged at two sides of the nucleic acid extraction disk transfer module 30, and extends from the first end of the nucleic acid extraction disk transfer module 30 to the opposite second end respectively; each conveyor belt 32 is respectively arranged above the track 31. The tray elastic support member 33 is arranged at the first end of the nucleic acid extraction tray conveying module 30; the tray support detection member 34 is arranged at the first end of the nucleic acid extraction tray conveying module 30 and is adjacent to the tray elastic support member 33; the tray in-position stopper 35 is arranged at the second end of the nucleic acid extraction tray conveying module 30; one of the two tray lifting safety detection members 36 is arranged at one side of the nucleic acid extraction tray conveying module 30 and adjacent to the track 31, and the other of the two tray lifting safety detection members 36 is arranged at the other side of the nucleic acid extraction tray conveying module 30 and adjacent to the track 31; the tray lifting assembly 37 is arranged between the two tracks 31 of the nucleic acid extraction disk conveying module 30, the carrier elastic support member 33 and the carrier in-place stopper 35; each carrier positioning pin 38 is respectively arranged diagonally at the first end and the second end of the nucleic acid extraction disk conveying module 30, and each carrier positioning pin can confirm that the position and direction of the carrier 50 placed in the nucleic acid extraction disk conveying module 30 are correct; the first carrier in-place detection member 39A is arranged at the second end of the nucleic acid extraction disk conveying module 30; each second carrier in-place detection member 39B is respectively arranged on the carrier lifting assembly 37 and the second end of the nucleic acid extraction disk conveying module 30.

[0067] During actual operation, the user places the carrier 50 with the nucleic acid extraction well plate on the conveyor belt 32, and pushes the carrier 50 toward the first end of the nucleic acid extraction plate transfer module 30 to the carrier elastic support member 33. At this time, the user can feel the elastic force of the carrier elastic support member 33 to position the carrier elastic support member 33; in addition, the carrier support detection member 34 can check whether the carrier 50 is actually in the correct position.

[0068] Then the automatic positioning process begins, the tray positioning block 35 rises to a limited position, and then the conveyor belt 32 conveys the tray 50 toward the second end of the nucleic acid extraction tray conveying module 30. When the tray 50 abuts against the tray positioning block 35, the conveying of the conveyor belt 32 is stopped. Then, the tray lifting safety detection member 36 moves toward the direction of the tray 50, and the tray lifting assembly 37 lifts the tray 50, and the tray positioning pins 38 are raised, so that the tray lifting safety detection member 36 is located directly above the tray 50, and each tray positioning pin 38 is inserted into the positioning hole of the tray 50. The tray lifting safety detection member 36 can detect whether the tray 50 is in the correct position to avoid the danger of the tray 50 in the wrong position or state for subsequent processes.

[0069] Finally, the tray 50 is transported. When the sample is completely arranged on the extraction well plate on the tray 50, the tray in place stopper 35 is lowered to a retracted position, and the tray lifting assembly 37 is lowered, so that the tray 50 is lowered onto the conveyor belt 32. After the first tray in place detection member 39A confirms that the tray 50 has indeed been lowered, the tray lifting safety detection member 36 is retracted, and the tray 50 is moved toward the second end of the nucleic acid extraction tray conveying module 30 and away from the tray lifting assembly 37 through the conveyor belt 32. While the tray 50 is continuously transported toward the nucleic acid extraction tray conveying module 30 in a direction away from the tray lifting assembly 37, the second tray in place detection member 39B can check the progress of the tray 50.

[0070] Although the present invention has been disclosed in terms of preferred embodiments, they are not intended to limit the present invention. Any person skilled in the art may make various changes and modifications without departing from the spirit and scope of the present invention. Therefore, the scope of protection of the present invention shall be determined by the scope of the attached patent application.

Claims

1. A combined nucleic acid pretreatment device, wherein: It includes a sampling and inspection pipe rack, which includes: An upper plate, comprising a plurality of first through holes and a plurality of upper guides, each of which is an annular hollow structure and is respectively disposed in the first through hole of the upper plate; an inner wall of each of which has an inclined surface; A lower plate is parallel to the upper plate, comprising a plurality of second through holes and a plurality of lower guides, one of the plurality of second through holes corresponds to one of the plurality of first through holes; each of the lower guides is respectively disposed in the second through hole of each of the lower plates, and each of the lower guides has a peripheral wall and an inclined surface, the peripheral wall surrounds and forms a receiving space; the inclined surface is disposed at the top end of the peripheral wall and shrinks from the peripheral wall toward the interior and axial direction of the lower guide; and Four brackets are respectively arranged between the upper plate and the lower plate, one end of each bracket is respectively fixed to one of the four corners of the lower plate, and the other end of each bracket is respectively fixed to one of the four corners of the upper plate.

2. The combined nucleic acid pretreatment device according to claim 1, wherein: The lower plate also includes a first positioning pin hole and a second positioning pin hole. The first positioning pin hole is arranged at one corner of the lower plate, and the second positioning pin hole is arranged at another corner of the lower plate.

3. The combined nucleic acid pretreatment device according to claim 2, wherein: The first positioning pin hole is diagonally arranged with respect to the second positioning pin hole, and the inner diameter of the first positioning pin hole is different from the inner diameter of the second positioning pin hole.

4. The combined nucleic acid pretreatment device according to claim 1, wherein: The height of the peripheral wall of the lower guide is greater than 8.3 mm, each of the lower guides has a guide angle height greater than 4 mm, and each of the lower guides has a guide angle θ ranging between 36° and 49.9°.

5. The combined nucleic acid pretreatment device according to claim 1, wherein: It also includes a nucleic acid extraction plate transmission module, and the nucleic acid extraction plate transmission module is arranged at a height lower than the sampling and inspection tube rack; The nucleic acid extraction disk transfer module comprises: Two tracks are disposed oppositely at two sides of the nucleic acid extraction disk conveying module and extend from a first end to an opposite second end of the nucleic acid extraction disk conveying module respectively; Two conveyor belts are respectively arranged above the track; a tray elastic support member, disposed at the first end of the nucleic acid extraction tray transfer module, and A carrier plate positioning detection member is disposed at the first end of the nucleic acid extraction plate transmission module and is adjacent to the carrier plate elastic positioning member.

6. The combined nucleic acid pretreatment device according to claim 5, wherein: The nucleic acid extraction plate transfer module further comprises a plate-carrying stopper, which is arranged at the second end of the nucleic acid extraction plate transfer module.

7. The combined nucleic acid pretreatment device according to claim 6, wherein: The nucleic acid extraction plate transfer module also includes two plate lifting safety detection parts, which are respectively arranged on one side of the nucleic acid extraction plate transfer module and adjacent to the track, and on the other side of the nucleic acid extraction plate transfer module and adjacent to the track.

8. The combined nucleic acid pretreatment device according to claim 7, wherein: The nucleic acid extraction plate conveying module further comprises a plate lifting assembly, which is arranged between two tracks of the nucleic acid extraction plate conveying module, the plate elastic positioning member and the plate positioning stopper.

9. The combined nucleic acid pretreatment device according to claim 8, wherein: The nucleic acid extraction plate transfer module further comprises at least two carrier plate positioning pins, and the at least two carrier plate positioning pins are respectively arranged diagonally at the first end and the second end of the nucleic acid extraction plate transfer module.

10. The combined nucleic acid pretreatment device according to claim 9, wherein: The nucleic acid extraction plate transfer module also includes a first carrier plate in place detection component and at least one second carrier plate in place detection component. The first carrier plate in place detection component is arranged at the second end of the nucleic acid extraction plate transfer module, and the at least one second carrier plate in place detection component is respectively arranged on the carrier plate lifting assembly and the second end of the nucleic acid extraction plate transfer module.

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