Bacterial colony transfer equipment

Abstract

The utility model discloses bacterial colony transfer equipment. The equipment comprises a transfer seat, an adsorption device, a first container, a second container and a third container, the adsorption device is arranged on the transfer seat, a plurality of carriers are arranged in the first container, and the second container is used for accommodating a plurality of bacterial colonies. The transfer seat can move to the first container so as to adsorb the carrier in the first container through the adsorption device, the transfer seat can drive the carrier to move to the second container and make contact with the corresponding bacterial colony, and the transfer seat can drive the carrier to move to the third container so as to transfer the bacterial colony attached to the carrier to the third container. After the bacterial colony transfer equipment adsorbs the carrier, the carrier can be driven to make contact with the bacterial colony, the carrier is put into the third container, the transfer base does not need to independently execute the step of discarding the carrier, the carrier does not need to be disinfected, cleaned and dried immediately, time is saved, the carrier can be recycled in the later period, and no carrier consumables are consumed in the whole experiment.

Description

Technical Field

[0001] The present application relates to the field of biotechnology, and in particular to a bacterial colony transfer device. Background Art

[0002] At present, in biological experiments such as biological genetic engineering and microbial culture, bacterial colony picking is mainly done manually. With the increasing number of applications, a bacterial colony picking experiment often requires picking thousands of cloned colonies, which is a large workload, low manual picking efficiency, and the selection of cloned colonies based on subjective judgment, which is easy to miss the target clone. Therefore, manual picking of bacterial colonies has gradually failed to meet the requirements of high-throughput experiments.

[0003] With the development of biotechnology, the traditional manual selection of bacterial colonies has gradually failed to meet the requirements of high-throughput screening. Using a bacterial selection instrument instead of manual automatic selection of bacterial colonies can not only improve the picking efficiency, but also greatly improve the picking accuracy.

[0004] In the related art, the bacterial colony picking instrument is provided with a plurality of picking pins, and the picking pins of the bacterial colony picking instrument can move on the three rectangular coordinate moving axes of X, Y, and Z. First, machine vision technology is adopted, and a camera is used to identify and locate the bacterial colonies in the culture dish, and the colonies are picked up by the picking pins, and then inoculated into the well plate. The picking pins cannot be put into the well plate, and the picking pins are usually directly discarded after inoculation, which causes a lot of waste. If the picking pins need to be recycled, it is often necessary to inoculate all the picking pins (usually 96) into the well plate at one time, and then the test is interrupted. These picking pins need to be uniformly disinfected, cleaned, and dried. After the processing is completed, the test can be restarted, which makes the test work time-consuming and is not conducive to improving the test efficiency. In the related art, the bacterial colony picking instrument uses a mechanical clamping mechanism to grab and release the picking pins. This mechanical clamping mechanism has a complex structure and general reliability, and the mechanical clamping mechanism performs actions slowly, which is not conducive to improving work efficiency. Utility Model Content

[0005] In order to solve one of the above technical defects, the first object of the present application is to provide a colony transfer device, comprising:

[0006] A transfer seat, wherein the transfer seat can be movably arranged;

[0007] An adsorption device, the adsorption device is arranged on the transfer seat;

[0008] a first container, wherein a plurality of carriers are disposed in the first container;

[0009] a second container, the second container being used to accommodate a plurality of bacterial colonies;

[0010] A third container, the third container is used for carrying bacteria;

[0011] The transfer seat can move to the first container to adsorb the carrier in the first container through the adsorption device, the transfer seat can drive the carrier to move to the second container and contact the corresponding bacterial colonies, and the transfer seat can drive the carrier to move to the third container to transfer the bacterial colonies adhered to the carrier to the third container.

[0012] Optionally, the adsorption device adsorbs the carrier by magnetic attraction and / or negative pressure adsorption.

[0013] Optionally, the adsorption device includes a shell, a magnetic hollow tube and a coil;

[0014] The housing is connected to the transfer seat;

[0015] The magnetically conductive hollow tube and the coil are both arranged in the shell, and the coil is sleeved in the magnetically conductive hollow tube;

[0016] The coil is energized to generate magnetic attraction to adsorb the carrier, and / or the magnetic hollow tube is connected to a negative pressure device to adsorb the carrier by negative pressure.

[0017] Optionally, the colony transfer device comprises a flexible end piece, wherein the end piece is connected to an end of the magnetically conductive hollow tube away from the housing;

[0018] The end piece has a receiving groove, and the receiving groove is connected to the hollow cavity of the magnetic hollow tube. When the adsorption device adsorbs the carrier, the carrier is at least partially embedded in the receiving groove.

[0019] Optionally, the magnetically conductive hollow tube is slidably connected to the shell;

[0020] The adsorption device includes an elastic member;

[0021] The elastic member is arranged between the shell and the magnetic conductor.

[0022] Optionally, a driving device is provided on the transfer seat;

[0023] The adsorption device is rotatably arranged on the transfer seat;

[0024] The driving device is transmission-connected to the adsorption device to drive the adsorption device to rotate.

[0025] Optionally, the colony transfer device includes a vibration device, and the first container has a plurality of limiting portions, and the limiting portions are through holes or grooves;

[0026] The vibration device can drive the first container to vibrate, so that the carrier in the first container can move and be limited at the limiting portion.

[0027] Optionally, the carrier has a large head end and a small head end;

[0028] The inner diameter of the limiting portion is larger than the outer diameter of the small head end and smaller than the outer diameter of the large head end;

[0029] When the carrier is limited in the limiting portion, the small end of the carrier is embedded in the limiting portion.

[0030] The second object of the present application is to provide a colony transfer method of the above-mentioned colony transfer device, comprising:

[0031] Step S1, controlling the transfer seat to move to the first container, and the transfer seat adsorbs the carrier in the first container through the adsorption device;

[0032] Step S2, controlling the transfer seat to move to the second container so that the carrier contacts the corresponding bacterial colony;

[0033] Step S3, controlling the transfer seat to move to the third container, and transferring the bacterial colonies adhered to the carrier to the third container.

[0034] Optionally, the colony transfer device has a vibration device, the first container has a plurality of limit parts, and step S1 includes:

[0035] Step S11, judging whether there is a carrier in each limiting part, if the judgment result is yes, proceeding to step S13, otherwise proceeding to step S12;

[0036] Step S12, controlling the vibration device to vibrate the second container so that the carrier is confined in the positioning hole;

[0037] Step S13, controlling the transfer seat to move to the carrier limited by the limiting portion and adsorbing the carrier;

[0038] Step S14, proceed to step S2.

[0039] By adopting the above technical solution, the present application has the following beneficial effects:

[0040] The colony transfer equipment of the present application adsorbs the carrier, contacts the colonies, and places the carrier in contact with the colonies into a third container. There is no need for a transfer seat to discard the carrier, and there is no need to immediately disinfect, clean, and dry the carrier, which saves time. The carrier can be recycled later, and there are no consumables in the entire experiment. BRIEF DESCRIPTION OF THE DRAWINGS

[0041] The accompanying drawings are part of this application and are used to provide a further understanding of the utility model. The schematic embodiments of the utility model and their descriptions are used to explain the utility model, but do not constitute an improper limitation of the utility model. Obviously, the drawings described below are only some embodiments. For ordinary technicians in this field, other drawings can be obtained based on these drawings without creative work.

[0042] Figure 1 A schematic diagram of the colony transfer process of the colony transfer device of the present application is shown.

[0043] Figure 2 A cross-sectional view showing the partial structure of the colony transfer device of the present application.

[0044] In the figure: 1. first container; 11. carrier; 2. second container; 21. colony; 3. third container; 4. transfer seat; 5. adsorption device; 51. shell; 511. gear ring; 52. magnetic hollow tube; 53. coil; 54. end piece; 541. receiving groove; 55. elastic piece; 6. driving device; 61. motor; 62. gear. DETAILED DESCRIPTION

[0045] In order to make the purpose, technical solutions and advantages of the embodiments of the utility model clearer, the technical solutions in the embodiments will be clearly and completely described below in conjunction with the drawings in the embodiments of the utility model. The following embodiments are used to illustrate the utility model but are not used to limit the scope of the utility model.

[0046] In the description of the present invention, it should be noted that the terms "upper", "lower", "inside", "outside", etc. indicate directions or positional relationships based on the directions or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific direction, be constructed and operated in a specific direction, and therefore cannot be understood as a limitation on the present invention.

[0047] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "installation" and "connection" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0048] Example 1

[0049] See also Figure 1 and Figure 2As shown, the embodiment of the present application provides a colony transfer device, including: a transfer seat 4, an adsorption device 5, a first container 1, a second container 2 and a third container 3. The transfer seat 4 can be movably arranged, and the adsorption device 5 is arranged on the transfer seat 4. A plurality of carriers 11 are arranged in the first container 1, the second container 2 is used to accommodate a plurality of colonies 21, and the third container 3 is used to carry the bacteria, and the third container 3 can be a well plate. The transfer seat 4 can move to the first container 1 to adsorb the carrier 11 in the first container 1 through the adsorption device 5, the transfer seat 4 can drive the carrier 11 to move to the second container 2 and contact the corresponding colony 21, the transfer seat 4 can drive the carrier 11 to move to the third container 3, and transfer the colony adhered to the carrier 11 to the third container 3, that is, the carrier 11 and the colony 21 adhered to the carrier 11 are simultaneously placed in the third container 3. The transfer seat 4 does not need to perform the action of discarding the carrier 11 alone, and the colony 21 and the carrier 11 participate in the later experiment at the same time.

[0050] The colony transfer device of the present application uses an adsorption device 5 to adsorb the carrier 11. Compared with other grasping structures, this adsorption method is more efficient and takes less time to grasp the carrier 11. After the adsorption device 5 adsorbs the carrier 11, the transfer seat continues to move so that the carrier 11 contacts the colony 21, and the carrier 11 with the bacteria attached is placed in the third container 3. The transfer seat 4 does not need to perform the step of discarding the carrier 11 separately, and the carrier 11 does not need to be disinfected, cleaned, and dried immediately, which saves time. The carrier 11 can be recycled later, and there is no carrier consumables in the entire experiment.

[0051] It should be noted that in the embodiment of the present application, the number of carriers 11 is unlimited. After the number of carriers in the first container is reduced to a certain number, the carrier delivery mechanism of the colony transfer device can directly deliver enough carriers into the first container. There is no need to stop the machine and perform a series of disinfection, cleaning and drying treatments on all carriers before continuing the test. Of course, the carriers can also be manually added to the first container. The first container of the present application can be disc-shaped, and the structural setting of the first container facilitates the unlimited replenishment of carriers.

[0052] Among them, the transfer seat 4 can move freely along the X-axis, Y-axis and Z-axis directions, and can drive the adsorption device 5 to move to different target positions, accurately performing the tasks of adsorption carrier, colony selection and colony transfer.

[0053] It should be noted that in the present application, the carrier 11 can be spherical, ellipsoidal, pyramidal, prism-shaped, pancake-shaped, polyhedral, etc. The carrier 11 can preferably adopt a spherical structure. The carrier 11 with a spherical structure has a regular structure, and the surrounding side structure has no difference in angle and shape, which is more conducive to matching with the adsorption device 5. The spherical carrier has a small circular contact surface at the bottom, which is conducive to selecting smaller-sized colonies 21, and is conducive to contacting, adhering to and carrying microorganisms. The bottom of the colony 21 in the second container 2 is a culture medium. Compared with the prior art of using a pick to pierce the colony 21, which is easy to contact the culture medium, in the present application, the carrier 11 adopts a spherical structure, and the carrier is directly dipped on the surface of the colony 21, and the carrier 11 is not easy to damage or adhere to the culture medium.

[0054] In the embodiment of the present application, the carrier 11 is preferably a sphere. Specifically, the carrier 11 can be a ferromagnetic metal sphere, a hard sphere or a flexible sphere. If the carrier is a ferromagnetic metal sphere, its surface can be covered with a flexible layer. The sphere can have a cavity, the sphere can have a smooth spherical surface, and the sphere can also have a plurality of protrusions / depressions on the surface. The present application does not limit the specific shape of the carrier 11.

[0055] In a possible implementation, the adsorption device 5 adsorbs the carrier 11 by magnetic attraction and / or negative pressure adsorption. If the magnetic adsorption method is adopted, the adsorption or release of the carrier 11 can be achieved by controlling the on and off of the circuit, which is simple to control, and the speed of grabbing or placing the carrier 11 is fast, which is conducive to improving work efficiency. If the negative pressure adsorption method is adopted, it is necessary to control the opening or closing of the negative pressure device to realize the grabbing or releasing of the carrier 11. Compared with the magnetic adsorption method, the adsorption method is more versatile and can be applied to grabbing carriers 11 of different materials.

[0056] In a possible embodiment, the adsorption device 5 includes a shell 51, a magnetic hollow tube 52 and a coil 53, the shell 51 is connected to the transfer seat 4, the magnetic hollow tube 52 and the coil 53 are both arranged in the shell 51, and the coil 53 is sleeved in the magnetic hollow tube 52, wherein the coil 53 is energized to generate magnetic attraction to adsorb the carrier 11, and / or the magnetic hollow tube 52 is connected to a negative pressure device to adsorb the carrier 11 under negative pressure.

[0057] In the embodiment of the present application, the adsorption device 5 can have both magnetic adsorption and negative pressure adsorption functions. When grabbing the carrier 11, the carrier 11 can be adsorbed by magnetic adsorption or negative pressure adsorption. When grabbing a carrier 11, the transfer seat 4 moves so that the magnetic hollow tube 52 is close to the target carrier 11, and the carrier 11 is adsorbed to the bottom of the magnetic hollow tube 52 under the action of magnetic attraction or negative pressure. When the carrier 11 is grabbed by magnetic attraction, magnetism can be generated by energizing the control coil 53. When the adsorption device 5 is close to the carrier 11 made of magnetic material, the carrier 11 will be adsorbed to the magnetic hollow tube 52. When the carrier 11 is grabbed by negative pressure adsorption, the negative pressure device is controlled to operate. When the bottom opening of the magnetic hollow tube 52 is close to or in contact with the carrier 11, the carrier 11 will be firmly adsorbed to the bottom opening of the magnetic hollow tube 52. In the present application, the adsorption device can simultaneously grasp the carrier 11 by magnetic attraction and negative pressure adsorption, thereby improving the stability of the carrier 11 being grasped and the carrier 11 will not fall easily.

[0058] In a possible embodiment, the colony transfer device includes a flexible end piece 54, and the end piece 54 is connected to the end of the magnetic hollow tube 52 away from the housing 51. The end piece 54 has a receiving groove 541, and the receiving groove 541 is connected to the hollow cavity of the magnetic hollow tube 52. The inner diameter of the receiving groove 541 is larger than the inner diameter of the magnetic hollow tube 52. When the adsorption device 5 adsorbs the carrier 11, the carrier 11 is at least partially embedded in the receiving groove 541.

[0059] The end piece 54 is set so that when the carrier 11 is adsorbed, the carrier 11 can be accommodated in the accommodation groove 541 of the end piece 54. The end piece 54 is a flexible structure and can be wrapped around the side of the carrier 11 to limit the position of the carrier 11. The end piece 54 will not be significantly deformed during the contact with the carrier 11. The flexible structure of the end piece 54 not only increases the friction between the end piece 54 and the carrier 11 to prevent the carrier 11 from moving, but also protects the carrier 11 and reduces the wear of the carrier 11. When the carrier 11 is adsorbed by negative pressure, the end piece 54 is set to seal the gap between the end piece 54 and the carrier 11, so that the carrier 11 can be stably adsorbed and fixed on the end piece 54.

[0060] In a possible implementation, the magnetic conductive hollow tube 52 is slidably connected to the housing 51, and the magnetic conductive hollow tube 52 can move up and down within a certain range along the housing 51. The adsorption device 5 includes an elastic member 55, and the elastic member 55 is arranged between the housing 51 and the magnetic conductive hollow tube 52. The elastic member 55 can be connected to the housing 51 and the magnetic conductive hollow tube 52 at both ends.

[0061] In this embodiment, by providing an elastic member 55, when the adsorption device 5 drives the carrier 11 downward to approach the colony 21, the carrier 11 will not be in rigid contact with the colony 21. When the carrier 11 contacts the colony 21 and presses down the colony 21, the elastic member 55 will elastically retract to prevent the carrier 11 from breaking up the colony 21 or being completely sunk into the colony 21.

[0062] In a possible implementation, a driving device 6 is disposed on the transfer seat 4, and the adsorption device 5 is rotatably disposed on the transfer seat 4. The driving device 6 and the adsorption device 5 are transmission-connected to drive the adsorption device 5 to rotate.

[0063] In the embodiment of the present application, when the transfer seat 4 moves to the point where the carrier 11 contacts the colony 21, the driving device 6 drives the adsorption device 5 to rotate, so that the carrier 11 rotates on the colony 21, which is beneficial for the colony 21 to adhere to the carrier 11, and the effect is significant. When the colony 21 needs to be placed in the third container 3, the carrier 11 can be controlled to contact the third container 3 and the adsorption device 5 can be controlled to drive the carrier 11 to rotate, so as to effectively transfer the colony to the third container 3 or cooperate with the soft carrier 11 to leave a uniform imprint on the third container 3. At this time, the carrier does not need to be placed in the third container.

[0064] In the embodiment of the present application, the driving device 6 can drive the adsorption device to rotate continuously in a single direction, and the driving device 6 can also drive the adsorption device to rotate reciprocatingly.

[0065] When the driving device 6 is required to drive the adsorption device to rotate in a single direction, the coil 53 and the power supply cable need to be connected through an electric slip ring or a similar structure to achieve a rotatable connection of the power supply cable of the coil 53 and maintain electrical contact to prevent the power supply cable from twisting. Similarly, the magnetic hollow tube 52 needs to be rotatably connected to the exhaust pipe in a dynamic seal to ensure that the exhaust pipe will not move with the rotation of the magnetic hollow tube 52 to prevent the exhaust pipe from twisting.

[0066] In order to simplify the structure, in the implementation of the present application, the driving device 6 can also only drive the adsorption device 5 to reciprocate within a smaller angle range, effectively avoiding the power supply cable and the exhaust pipe from twisting. The smaller angle range can be within 180 degrees. In this way, there is no need to connect the coil 53 and the power supply cable through a complex structure such as an electric slip ring, and there is no need to connect the magnetic hollow tube 52 and the exhaust pipe with a complex dynamic sealing structure, thereby reducing costs.

[0067] The driving device 6 may include a motor 61 and a transmission assembly, the motor 61 and the transmission assembly are in transmission connection, the transmission assembly and the adsorption device 5 are in transmission connection, and the rotation of the motor 61 drives the adsorption device 5 to rotate through the transmission assembly. The motor 61 may only perform reciprocating rotation within a small angle range.

[0068] The transmission assembly may include a belt or a gear 62. For example, a gear ring 511 is provided on the housing 51, and the gear ring 511 is extended around the rotation axis of the housing 51. The transmission assembly may include a gear 62 connected to the rotating shaft of the motor 61. The gear 62 and the gear ring 511 are meshed. The motor 61 drives the gear 62 to rotate, and the gear 62 can drive the gear ring 511 to rotate. When the transmission assembly includes a belt, the belt can be sleeved on the rotating shaft of the motor 61 and the housing 51 of the adsorption device 5. The motor 61 rotates, and the adsorption device 5 is driven to rotate through the belt.

[0069] In some optional embodiments, the colony transfer device includes a vibration device, the first container 1 has a plurality of limiting parts, the limiting parts are through holes or grooves, and the vibration device can drive the first container 1 to vibrate, so that the carrier 11 in the first container 1 can move and be limited to the limiting part. The vibration device can be connected to the first container 1 in a transmission manner, and the vibration device drives the first container 1 to move. The movement mode of the first container 1 can be reciprocating motion, circular motion, cross motion or polarized motion (including simultaneous up and down motion). The vibration device can include a knocking piece, and the vibration device controls the knocking piece to perform a knocking action on the first container 1, so that the carrier 11 in the first container 1 jumps, which is conducive to the carrier 11 being limited to the limiting part.

[0070] When the carrier 11 is limited in the limiting portion, the position of the carrier 11 is fixed, which facilitates the adsorption device 5 to smoothly adsorb the carrier 11. The colony transfer device can have a first image sensor, which is used to capture the image of the first container 1 and obtain the position information of each carrier 11. The transfer seat 4 can control the position of the mobile adsorption device 5 according to the position information of each carrier 11, so that the adsorption device 5 successfully adsorbs the target carrier 11. The first image sensor can be fixedly set, and the first image sensor can be located at the top of the first container 1, and does not interfere with the movement of the transfer seat 4.

[0071] In addition to being spherical, the carrier 11 may also be in other shapes. For example, the carrier 11 may have a large end and a small end. The large end and the small end may transition smoothly. The inner diameter of the limiting portion is larger than the outer diameter of the small end and smaller than the outer diameter of the large end. When the carrier 11 is limited in the limiting portion, the small end of the carrier 11 is embedded in the limiting portion. In view of the special shape of the carrier 11, when the vibration device vibrates the first container 1, each carrier 11 jumps in the first container 1 until the small end of the carrier 11 is inserted into the limiting portion. Only then can the position of the carrier 11 be fixed, with the small end facing downward and the large end facing upward, which is more conducive to being adsorbed by the adsorption device 5.

[0072] A second image sensor may be provided on the transfer seat 4 , and the second image sensor is used to obtain image data of the colony 21 , identify the target colony 21 , and thereby assist the transfer seat 4 to move to a suitable position so that the carrier 11 can successfully contact the target colony 21 .

[0073] The colony transfer equipment includes a third image sensor, which is located below the transfer seat 4. After the transfer seat 4 adsorbs the carrier 11, in the process of moving from the first container to the second container, the transfer seat 4 passes over the third image sensor. The third image sensor captures the image upwards and obtains the image of the adsorption device 5 on the transfer seat 4, which is used to determine whether the carrier 11 is effectively adsorbed on the adsorption device 5. If it is detected that the carrier 11 is not adsorbed on the adsorption device 5, the subsequent transfer task can be interrupted.

[0074] It should be noted that in the present application, only one adsorption device can be set on the transfer seat, or two, three or more adsorption devices can be set on the transfer seat at the same time. During the movement of the transfer seat, each adsorption device can adsorb the carrier, thereby significantly improving the colony transfer efficiency.

[0075] Example 2

[0076] Embodiment 2 of the present application provides a colony transfer method of the colony transfer device in Embodiment 1, including:

[0077] Step S1, controlling the transfer seat 4 to move to the first container 1, and the transfer seat 4 adsorbs the carrier 11 in the first container 1 through the adsorption device 5;

[0078] Before step S1, the vibration device can be controlled to vibrate the first container 1, so that at least part of the limiting parts in the first container 1 are limited by the carrier 11, which is convenient for the transfer seat 4 to accurately adjust the coordinates and facilitates the adsorption device 5 to successfully adsorb the carrier 11. During the continuous operation of the colony transfer device, the transfer seat 4 adjusts the position of the adsorption device 5 in sequence, and successively adsorbs and transfers the carriers 11 limited in different limiting parts. After the carriers 11 in all limiting parts are adsorbed and transferred, the vibration device can be controlled to vibrate the first container 1, so that the carrier 11 moves, until the carrier 11 is limited in part of the limiting parts.

[0079] Step S2, control the transfer seat 4 to move to the second container 2, so that the carrier 11 contacts the corresponding colony 21; in this step, the image data of the colony 21 can be obtained by the second image sensor, and the target colony 21 can be identified, thereby assisting the transfer seat 4 to move to a suitable position, so that the carrier 11 can successfully contact the target colony 21. When the carrier 11 contacts the target colony 21, the driving device 6 also drives the adsorption device 5 to rotate, which is conducive to the colony 21 adhering to the carrier 11, and the effect is significant.

[0080] It should be noted that the colony transfer equipment includes a third image sensor, which is located below the transfer seat 4. After the transfer seat 4 adsorbs the carrier 11, during the process of moving from the first container to the second container, the transfer seat 4 passes over the third image sensor. The third image sensor captures the image upwards to obtain the image of the adsorption device 5 on the transfer seat 4, which is used to determine whether the carrier 11 is effectively adsorbed on the adsorption device 5. If it is detected that the carrier 11 is not adsorbed on the adsorption device 5, step S2 will no longer be executed.

[0081] Step S3 , controlling the transfer seat 4 to move to the third container 3 , and transferring the bacterial colonies adhered to the carrier 11 to the third container 3 .

[0082] In this step, the adsorption device 5 can directly put the carrier 11 into the third container 3, and the adsorption device 5 does not separately perform the action of discarding the carrier 11 to other locations, which saves time.

[0083] Alternatively, when the colony 21 needs to be placed in the third container 3, the carrier 11 can be controlled to contact the third container 3 and the adsorption device 5 can be controlled to drive the carrier 11 to rotate, so as to effectively transfer the colony to the third container 3. At this time, the carrier does not need to be placed in the third container.

[0084] Optionally, the colony transfer device has a vibration device, the first container 1 has a plurality of limit parts, and step S1 includes:

[0085] Step S11, judging whether there is a carrier 11 in each limiting part, if the judgment result is yes, proceeding to step S13, otherwise proceeding to step S12;

[0086] Step S12, controlling the vibration device to vibrate the second container 2 so that the carrier 11 is confined in the positioning hole;

[0087] Step S13, controlling the transfer seat 4 to move to the carrier 11 limited by the limiting portion and adsorbing the carrier 11;

[0088] Step S14, proceed to step S2.

[0089] The preferred embodiments of the present application disclosed above are only used to help explain the present application. The preferred embodiments do not describe all the details in detail, nor do they limit the present application to the specific implementation methods described. Obviously, many modifications and changes can be made according to the content of this specification. This specification selects and specifically describes these embodiments in order to better explain the principles and practical applications of the present application, so that those skilled in the art can understand and use the present application well. The present application is limited only by the claims and their full scope and equivalents.

Claims

1. A bacterial colony transfer device, characterized in that: include: A transfer seat, wherein the transfer seat can be movably arranged; An adsorption device, the adsorption device is arranged on the transfer seat; a first container, wherein a plurality of carriers are disposed in the first container; a second container, the second container being used to accommodate a plurality of bacterial colonies; A third container, the third container is used for carrying bacteria; The transfer seat can move to the first container to adsorb the carrier in the first container through the adsorption device, the transfer seat can drive the carrier to move to the second container and contact the corresponding bacterial colonies, and the transfer seat can drive the carrier to move to the third container to transfer the bacterial colonies adhered to the carrier to the third container.

2. The colony transfer device according to claim 1, characterized in that: The adsorption device adsorbs the carrier by magnetic attraction and / or negative pressure adsorption.

3. The colony transfer device according to claim 1, characterized in that: The adsorption device comprises a shell, a magnetic hollow tube and a coil; The housing is connected to the transfer seat; The magnetically conductive hollow tube and the coil are both arranged in the shell, and the coil is sleeved in the magnetically conductive hollow tube; The coil is energized to generate magnetic attraction to adsorb the carrier, and / or the magnetic hollow tube is connected to a negative pressure device to adsorb the carrier by negative pressure.

4. The colony transfer device according to claim 3, characterized in that: It comprises a flexible terminal piece, and the terminal piece is connected to one end of the magnetic hollow tube away from the shell; The end piece has a receiving groove, and the receiving groove is connected to the hollow cavity of the magnetic hollow tube. When the adsorption device adsorbs the carrier, the carrier is at least partially embedded in the receiving groove.

5. The colony transfer device according to claim 3, characterized in that: The magnetically conductive hollow tube is slidably connected to the shell; The adsorption device comprises an elastic member; The elastic member is arranged between the shell and the magnetically conductive hollow tube.

6. The colony transfer device according to claim 3, characterized in that: A driving device is arranged on the transfer seat; The adsorption device is rotatably arranged on the transfer seat; The driving device is transmission-connected to the adsorption device to drive the adsorption device to rotate.

7. The colony transfer device according to any one of claims 1 to 6, characterized in that: The vibrating device is included, wherein the first container has a plurality of limiting parts, and the limiting parts are through holes or grooves; The vibration device can drive the first container to vibrate, so that the carrier in the first container can move and be limited at the limiting portion.

8. The colony transfer device according to claim 7, characterized in that: The carrier has a large head end and a small head end; The inner diameter of the limiting portion is larger than the outer diameter of the small head end and smaller than the outer diameter of the large head end; When the carrier is limited in the limiting portion, the small end of the carrier is embedded in the limiting portion.

Images