Visible component floating enrichment chip
By designing a floating enrichment chip with a forming part, the internal structure of the chip is used to achieve the aggregation and enrichment of floating particulates, the problem of low analysis efficiency in the prior art is solved and the analysis efficiency and accuracy are improved.
Patent Information
- Application Number
- CN202420933813.5
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-04-30
- Publication Date
- 2025-02-14
- Estimated Expiration
- 2034-04-30
AI Technical Summary
In the prior art, floating state formation parts are difficult to effectively aggregate and quantitatively analyze within the imaging area of the microscopic sample, resulting in low analysis efficiency.
A floating enrichment chip with formation components is designed. Through the structural design of the chip, including accommodating cavity, an observation window, A-side cavity and B-side cavity, the aggregation and enrichment of floating formation components is achieved. The design of the observation window allows the floating state to form upwardly, and the A-side cavity and the B-side cavity are used to receive the overflowing suspension to avoid loss of enrichment.
Through the internal structural design of the chip, efficient aggregation and enrichment of floating form components is achieved, external centrifugal enrichment steps are reduced, analysis efficiency is improved, and quantitative analysis accuracy is ensured.
Smart Images
Figure CN222481947U_ABST
Abstract
Description
Technical Field
[0001] The present application belongs to the technical field of formed element analysis, and specifically relates to a formed element analysis device and chip based on microscopic sample examination. Background Art
[0002] In the application of formed component analysis based on microscopic samples, i.e. formed component suspensions, the formed components in different suspensions are different. Feces, blood, and urine are three common suspensions, and there may be some formed components floating on the top of the suspension. The content or concentration of these floating formed components in the sample is usually low, but these floating formed components are very important for the composition analysis of the sample. How to aggregate these floating formed components within the limited imaging area of the microscopic sample and accurately perform quantitative analysis is a technical problem to be solved.
[0003] In the prior art, the samples are usually enriched outside the chip by centrifugation or other methods, and then injected into the chip for imaging and detection analysis, which reduces the efficiency of the entire process. Summary of the invention
[0004] In order to solve the problem of low efficiency of floating formed element aggregation imaging analysis in the prior art, this application proposes a formed element floating enrichment chip, which can achieve the aggregation of floating formed elements through the internal structural design of the chip and improve the aggregation efficiency of floating formed elements.
[0005] The technical solution of the present application to solve the above-mentioned technical problems is a formed element floating enrichment chip, including a chip body, a containing cavity, and an observation window; a containing cavity is provided on the chip body; the containing cavity is used to hold microscopic samples; the observation window is provided at the top of the containing cavity, and the area of the observation window is smaller than the average cross-sectional area of the containing cavity.
[0006] A removable cover is arranged above the observation window for covering the observation window.
[0007] The upper part of the chip body also includes a side cavity A and a side cavity B; the side cavity A and the side cavity B are respectively arranged above the accommodating cavity; the height of the side cavity A and the side cavity B is less than the height of the accommodating cavity; the side cavity A and the side cavity B are both connected to the accommodating cavity.
[0008] The cover sheet covers the A-side cavity and the B-side cavity.
[0009] The accommodating cavity comprises a lower cavity and an upper cavity, the upper cavity is arranged above the lower cavity, and the volume of the lower cavity is greater than that of the upper cavity.
[0010] The cross section of the lower cavity is larger than the cross section of the upper cavity.
[0011] The formed component floating enrichment chip also includes a sample adding port and an air outlet; the sample adding port is connected to the containing cavity, and the air outlet is connected to the containing cavity; the sample adding port and the air outlet are respectively arranged on one side of the containing cavity.
[0012] The horizontal position of the observation window is lower than the horizontal position of the sample adding port, and the horizontal position of the observation window is lower than the position of the gas outlet.
[0013] The sample adding port is connected to the bottom of the accommodating cavity through the sample adding channel; the gas outlet is connected to the upper part of the accommodating cavity through the gas outlet channel; or the sample adding port is connected to the bottom of the accommodating cavity through the sample adding channel; the gas outlet is connected to the bottom of the accommodating cavity through the gas outlet channel.
[0014] The sample adding port is communicated with the upper cavity of the accommodating cavity through the sample adding channel; the gas outlet is communicated with the upper cavity of the accommodating cavity through the gas outlet channel.
[0015] One of the technical effects of the above technical solution is: the observation window is set at the top of the containing cavity, and the area of the observation window is smaller than the average cross-sectional area of the containing cavity. The floating shaped components in the containing cavity are gathered upward at the top through the small-area observation window for subsequent microscopic imaging analysis. They can be directly enriched inside the chip, which can reduce the steps of separate external centrifugation enrichment or other enrichment.
[0016] One of the technical effects of the above technical solution is that the removable cover can cover the observation window to facilitate subsequent imaging observation. At the same time, the floating formed components can be adsorbed and fixed through the surface tension of the cover to facilitate observation, thereby reducing the impact of the movement of the floating formed components on microscopic imaging.
[0017] One of the technical effects of the above technical solution is that both the A-side cavity and the B-side cavity are connected to the containing cavity, and can receive the suspension with floating formed components overflowing from the top of the containing cavity, and can receive it in an overflow state to avoid the loss of enriched floating formed components.
[0018] One of the technical effects of the above technical solution is that the cover sheet covers the A-side cavity and the B-side cavity, so that the A-side cavity and the B-side cavity can also be conveniently used for microscopic observation.
[0019] One of the technical effects of the above technical solution is that the volume of the lower cavity is larger than that of the upper cavity, which facilitates the enrichment of a large sample volume and improves the enrichment ratio.
[0020] One of the technical effects of the above technical solution is that the cross-section of the lower cavity is larger than the cross-section of the upper cavity, which can further facilitate the enrichment of a large sample volume and improve the enrichment rate.
[0021] One of the technical effects of the above technical solution is that the sample loading port and the air outlet are respectively arranged on each side of the accommodating cavity, providing a separate sample loading channel and air outlet channel; the sample loading channel is separately arranged to avoid the inconvenience of sharing the sample loading and observation windows, and the enrichment state can be observed while the sample is loaded. The arrangement of the air outlet avoids the formation of bubbles in the observation window, which affects the enrichment of floating formed components and the subsequent microscopic imaging.
[0022] One of the technical effects of the above technical solution is that the horizontal position of the observation window is lower than the horizontal position of the sample adding port, and the horizontal position of the observation window is lower than the position of the air outlet, which is conducive to the discharge of bubbles through the air outlet.
[0023] One of the technical effects of the above technical solution is that: the sample adding port is connected to the bottom of the containing cavity through the sample adding channel; the air outlet is connected to the upper part of the containing cavity through the air outlet channel; the sample is added into the containing cavity through the sample adding channel and floats upward from the bottom of the containing cavity, so that the movement process is conducive to the enrichment of floating tangible components. The air outlet is connected to the upper part of the containing cavity through the air outlet channel, which is convenient for the discharge of air.
[0024] One of the technical effects of the above technical solution is that the sample adding port is connected to the upper cavity of the containing cavity through the sample adding channel; the gas outlet is connected to the upper cavity of the containing cavity through the gas outlet channel, which is convenient for sample adding and gas discharge. BRIEF DESCRIPTION OF THE DRAWINGS
[0025] Figure 1 is a perspective schematic diagram of embodiment 1 of the formed element floating enrichment chip;
[0026] Figure 2 is a perspective schematic diagram of embodiment 2 of the formed element floating enrichment chip;
[0027] Figure 3 is a perspective schematic diagram of embodiment 3 of the formed element floating enrichment chip;
[0028] Figure 4 is a perspective schematic diagram of Example 4 of a formed element floating enrichment chip;
[0029] Figure 5 is a perspective schematic diagram of embodiment 5 of the formed element floating enrichment chip;
[0030] Figure 6 is a top perspective schematic diagram of embodiment 5 of the formed element floating enrichment chip;
[0031] Figure 7 is a side perspective schematic diagram of embodiment 5 of the formed element floating enrichment chip;
[0032] Figure 8 is a side view schematic diagram of embodiment 6 of the formed element floating enrichment chip;
[0033] Fig. 9 is a top view schematic diagram of embodiment 6 of the formed element floating enrichment chip;
[0034] Fig.10 is a side view schematic diagram of embodiment 7 of the formed element floating enrichment chip;
[0035] Fig.11 is a top view schematic diagram of embodiment 8 of the formed element floating enrichment chip;
[0036] Fig.12 is a perspective schematic diagram of Example 9 of the formed element floating enrichment chip;
[0037] Fig.13 is a top perspective schematic diagram of embodiment 9 of the formed element floating enrichment chip;
[0038] Fig.14 is a side perspective schematic diagram of embodiment 9 of the formed element floating enrichment chip;
[0039] Fig.15 is a perspective schematic diagram of embodiment 10 of the formed element floating enrichment chip;
[0040] Fig.16 is a side perspective schematic diagram of embodiment 10 of the formed element floating enrichment chip;
[0041] Fig.17 is a perspective schematic diagram of embodiment 11 of the formed element floating enrichment chip;
[0042] Fig.18 It is a side perspective schematic diagram of embodiment 11 of the formed element floating enrichment chip. DETAILED DESCRIPTION
[0043] The content of this application is further described in detail below in conjunction with the accompanying drawings.
[0044] It should be noted that the following is a description of the preferred embodiments of the present application, which does not constitute any limitation to the present application. The description of the preferred embodiments of the present application is only an explanation of the general principles of the present application. The embodiments described in this application are only a part of the embodiments of the present application, not all of the embodiments. Based on the embodiments in this application, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of this application.
[0045] In the description of the present application, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise", etc., indicating the orientation or position relationship, are based on the orientation or position relationship shown in the drawings, and are only for the convenience of describing the present application and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present application. In addition, the terms "first", "second", and technical features numbered with Arabic numerals 1, 2, 3, etc., and numbers such as "A" and "B", are only used for descriptive purposes and are only for the convenience of explanation, and do not represent a sequential relationship in time or space; they cannot be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined as "first", "second", and numbered with Arabic numerals 1, 2, 3, etc. may explicitly or implicitly include one or more of the features. In the description of the present application, “several” means two or more than two, unless otherwise clearly and specifically defined.
[0046] like Figure 1 , a formed element floating enrichment chip 100, comprising a chip body 101, a containing cavity 110, and an observation window 130; a containing cavity 110 is provided on the chip body 101; the containing cavity 110 is used to hold microscopic samples; the observation window 130 is provided at the top of the containing cavity 110, and the area of the observation window 130 is smaller than the average cross-sectional area of the containing cavity 110. The area range of the observation window 130 is 0.5 square centimeters to 2 square centimeters. The average cross-sectional area means the area value obtained by dividing the cavity volume by the cavity height. The containing cavity 110 comprises a lower cavity 112 and an upper cavity 111, and the upper cavity 111 is provided above the lower cavity 112, and the volume of the lower cavity 112 is greater than the volume of the upper cavity 111. The cross-section of the lower cavity 112 is larger than the cross-section of the upper cavity 111. As Figures 1 to 4 The upper part of the upper cavity 111 is a prismatic accommodation space, and the lower part of the upper cavity 111 is a prism-shaped accommodation space. In other embodiments not shown in the drawings, the upper cavity 111 and the lower cavity 112 can also be both prismatic accommodation spaces.
[0047] exist Figure 1 In the embodiment of the present invention, the observation window 130 is also used as a sample addition port. The larger the volume of the accommodating cavity 110, the higher the enrichment rate; the smaller the area of the observation window 130, the higher the enrichment rate. The volume of the accommodating cavity 110 and the area of the observation window 130 can be set according to actual needs. In some embodiments, the height of the accommodating cavity 110 can be 0.5 mm to 50 mm.
[0048] like Figure 2 A removable cover sheet is disposed above the observation window 130. The removable cover sheet is a top cover sheet 131, which is used to cover the observation window 130. The top cover sheet 131 can be a small-area cover sheet that matches the size of the observation window 130, or a large-area cover sheet that covers the entire top surface of the chip body 101. The cover sheet can also be a non-removable, fixed cover sheet.
[0049] like Figure 3 The upper part of the chip body 101 also includes an A-side cavity 150 and a B-side cavity 160; the A-side cavity 150 and the B-side cavity 160 are respectively arranged above the accommodating cavity 110; the A-side cavity 150 and the B-side cavity 160 are smaller than the height of the accommodating cavity 110; the A-side cavity 150 and the B-side cavity 160 are both connected to the accommodating cavity 110, and the connecting channel is Figure 3 The A cover sheet 155 covers the top surface of the A-side cavity 150 , and the B cover sheet 165 covers the top surface of the B-side cavity 160 .
[0050] like Figure 3 The A cover sheet 155 can be a small-area cover sheet that matches the top surface area of the A-side cavity 170, and the B cover sheet 165 can be a small-area cover sheet that matches the top surface area of the B-side cavity 160. The A cover sheet 155, the B cover sheet 165 and the top cover sheet 131 can also be integrated, and are large-area cover sheets that cover the entire top surface of the chip body 101.
[0051] like Figure 4 A formed element floating enrichment chip further includes a sample adding port 175 and an air outlet 185; the sample adding port 175 is connected to the containing cavity 110, and the air outlet 185 is connected to the containing cavity 110; the sample adding port 175 and the air outlet 185 are respectively arranged on one side of the containing cavity 110. The sample adding port 175 is connected to the upper cavity 111 of the containing cavity 110 through the sample adding channel 170; the air outlet 185 is connected to the upper cavity 111 of the containing cavity 110 through the air outlet channel 180.
[0052] like Figures 5 to 7 A formed element floating enrichment chip 200 includes a chip body 201, a containing cavity 210, and an observation window 230; the chip body 201 is provided with a containing cavity 210; the containing cavity 210 is used to hold microscopic samples; the observation window 230 is provided at the top of the containing cavity 210, and the area of the observation window 230 is smaller than the average cross-sectional area of the containing cavity 210. The containing cavity 210 includes a lower cavity 212 and an upper cavity 211, and the upper cavity 211 is provided above the lower cavity 212, and the volume of the lower cavity 212 is larger than the volume of the upper cavity 211. The cross-section of the lower cavity 212 is larger than the cross-section of the upper cavity 211. Figures 5 to 7, the lower cavity 212 is a cylindrical accommodation space. The upper part of the upper cavity 211 is a cylindrical accommodation space, and the lower part of the upper cavity 211 is a truncated cone accommodation space. In some other embodiments, the upper cavity 211 can be a truncated cone accommodation space.
[0053] like Figures 5 to 7 The upper part of the chip body 201 also includes an A-side cavity 270 and a B-side cavity 260; the A-side cavity 270 and the B-side cavity 260 are respectively arranged above the accommodating cavity 210; the height of the A-side cavity 270 and the B-side cavity 260 is less than the height of the accommodating cavity 210; the A-side cavity 270 and the B-side cavity 260 are both connected to the accommodating cavity 210, and the connecting channel is Figure 5 The top surface 272 of the A-side cavity 150 , the top surface 262 of the B-side cavity 160 , and the observation window 230 are covered by a removable cover sheet 231 .
[0054] like Figures 8 to 11 A formed element floating enrichment chip 300 includes a chip body, a containing cavity 310, and an observation window 330; the chip body is provided with a containing cavity 310; the containing cavity 310 is used to hold a microscopic sample; the observation window 330 is provided at the top of the containing cavity 310, and the area of the observation window 330 is smaller than the average cross-sectional area of the containing cavity 310. The containing cavity 310 includes a lower cavity 312 and an upper cavity 311, the upper cavity 311 is provided above the lower cavity 312, and the volume of the lower cavity 312 is greater than the volume of the upper cavity 311. The cross section of the lower cavity 312 is greater than the cross section of the upper cavity 311. The upper cavity 311 and the lower cavity 312 are connected to form a truncated cone-shaped containing space.
[0055] like Figures 8 to 11 A formed element floating enrichment chip 300 includes a sample adding port 361 and an air outlet 371; the sample adding port 361 is connected to the containing cavity 310, and the air outlet 371 is connected to the containing cavity 310; the sample adding port 361 and the air outlet 371 are respectively arranged on one side of the containing cavity 310. The sample adding port 361 is connected to the bottom of the containing cavity 310 through the sample adding channel 360; the air outlet 371 is connected to the bottom of the containing cavity 310 through the air outlet channel 370.
[0056] like Fig.10 The observation window 330 is covered by a removable cover sheet 335 , and the floating formed components 900 gather at the observation window 330 . The sample addition port 361 is covered by a removable sample addition port cover sheet 365 , and the gas outlet 371 is covered by a removable sample addition port cover sheet 375 .
[0057] like Figures 8 to 11 The horizontal position of the observation window 330 is lower than the horizontal position of the sample adding port 361 , and the horizontal position of the observation window 330 is lower than the position of the gas outlet 371 .
[0058] like Figure 12 to Figure 14 A formed element floating enrichment chip 500 includes a chip body, a containing cavity 510, and an observation window 530; the chip body is provided with a containing cavity 510; the containing cavity 510 is used to hold a microscopic sample; the observation window 530 is provided at the top of the containing cavity 510, and the area of the observation window 530 is smaller than the average cross-sectional area of the containing cavity 510. The containing cavity 510 includes a lower cavity 512 and an upper cavity 511, the upper cavity 511 is provided above the lower cavity 512, and the volume of the lower cavity 512 is greater than the volume of the upper cavity 511. The cross-section of the lower cavity 512 is greater than the cross-section of the upper cavity 511. The upper cavity 511 and the lower cavity 512 are connected to form a truncated cone-shaped containing space.
[0059] like Figure 12 to Figure 14 A formed element floating enrichment chip 500 includes a sample adding port 561 and an air outlet 571; the sample adding port 561 is connected to the containing cavity 510, and the air outlet 571 is connected to the containing cavity 510; the sample adding port 561 and the air outlet 571 are respectively arranged on one side of the containing cavity 510. The sample adding port 561 is connected to the bottom of the containing cavity 510 through the sample adding channel 560; the air outlet 571 is connected to the bottom of the containing cavity 510 through the air outlet channel 570.
[0060] like Figure 15 to Figure 16 A formed element floating enrichment chip 600 includes a chip body, a containing cavity 610, and an observation window 630; the chip body is provided with a containing cavity 610; the containing cavity 610 is used to hold microscopic samples; the observation window 630 is provided at the top of the containing cavity 610, and the area of the observation window 630 is smaller than the average cross-sectional area of the containing cavity 610. The containing cavity 610 includes a lower cavity 612 and an upper cavity 611, the upper cavity 611 is provided above the lower cavity 612, and the volume of the lower cavity 612 is greater than the volume of the upper cavity 611. The cross section of the lower cavity 612 is greater than the cross section of the upper cavity 611. The upper cavity 611 is a truncated cone-shaped containing space, and the lower cavity 612 is a cylindrical containing space. The upper cavity 611 and the lower cavity 612 are connected.
[0061] like Figure 15 to Figure 16 A formed element floating enrichment chip 600 includes a sample adding port 661 and an air outlet 671; the sample adding port 661 is connected to the containing cavity 610, and the air outlet 671 is connected to the containing cavity 610; the sample adding port 661 and the air outlet 671 are respectively arranged on one side of the containing cavity 610. The sample adding port 661 is connected to the upper cavity 611 of the containing cavity 610 through the sample adding channel 660; the air outlet 671 is connected to the upper cavity 611 of the containing cavity 610 through the air outlet channel 670.
[0062] like Figure 17 to Figure 18A formed element floating enrichment chip 700 includes a chip body, a containing cavity 710, and an observation window 730; the chip body is provided with a containing cavity 710; the containing cavity 710 is used to hold microscopic samples; the observation window 730 is arranged at the top of the containing cavity 710, and the area of the observation window 730 is smaller than the average cross-sectional area of the containing cavity 710. The containing cavity 710 includes a lower cavity 712 and an upper cavity 711, the upper cavity 711 is arranged above the lower cavity 712, and the volume of the lower cavity 712 is greater than the volume of the upper cavity 711. The cross section of the lower cavity 712 is greater than the cross section of the upper cavity 711. The upper cavity 711 is a truncated cone-shaped containing space, and the lower cavity 712 is a cylindrical containing space. The upper cavity 711 and the lower cavity 712 are connected.
[0063] like Figure 17 to Figure 18 A formed element floating enrichment chip 700 includes a sample adding port 771 and an air outlet 761; the sample adding port 771 is connected to the containing cavity 710, and the air outlet 761 is connected to the containing cavity 710; the sample adding port 771 and the air outlet 761 are respectively arranged on one side of the containing cavity 710. The sample adding port 771 is connected to the bottom of the containing cavity 710, i.e., the lower cavity 712, through the sample adding channel 770; the air outlet 761 is connected to the upper part of the containing cavity 610, i.e., the upper cavity 711, through the air outlet channel 760.
[0064] Although the present application is illustrated and described according to the preferred embodiment and several alternatives, the application will not be limited by the specific description in this specification. Other additional replacement or equivalent components can also be used to practice the present application.
Claims
1. A shaped element floating enrichment chip, characterized in that: It includes a chip body, a containing cavity, and an observation window; A accommodating cavity is provided on the chip body; the accommodating cavity is used to hold microscopic examination samples; The observation window is set at the top of the accommodating cavity. The area of the observation window is smaller than the average cross-sectional area of the accommodating cavity.
2. The formed element floating enrichment chip according to claim 1, characterized in that: A removable cover is arranged above the observation window for covering the observation window.
3. The formed element floating enrichment chip according to claim 2, characterized in that: The upper portion of the chip body also includes an A-side cavity and a B-side cavity; The A-side cavity and the B-side cavity are respectively arranged above the accommodating cavity; The heights of the A-side cavity and the B-side cavity are smaller than the height of the accommodating cavity; The A-side cavity and the B-side cavity are both communicated with the accommodating cavity.
4. The formed element floating enrichment chip according to claim 3, characterized in that: The cover sheet covers the A-side cavity and the B-side cavity.
5. The formed element floating enrichment chip according to claim 1, characterized in that: The accommodating cavity comprises a lower cavity and an upper cavity, the upper cavity is arranged above the lower cavity, and the volume of the lower cavity is greater than that of the upper cavity.
6. The formed element floating enrichment chip according to claim 5, characterized in that: The cross section of the lower cavity is larger than the cross section of the upper cavity.
7. The formed element floating enrichment chip according to claim 1, characterized in that: It also includes a sample adding port and an air outlet; the sample adding port is connected to the containing cavity, and the air outlet is connected to the containing cavity; the sample adding port and the air outlet are respectively arranged on one side of the containing cavity.
8. The formed element floating enrichment chip according to claim 7, characterized in that: The horizontal position of the observation window is lower than the horizontal position of the sample adding port, and the horizontal position of the observation window is lower than the position of the gas outlet.
9. The formed element floating enrichment chip according to claim 1, characterized in that: The sample adding port is connected to the bottom of the containing cavity through the sample adding channel; the gas outlet is connected to the upper part of the containing cavity through the gas outlet channel; Or the sample adding port is connected to the bottom of the containing cavity through the sample adding channel; the gas outlet is connected to the bottom of the containing cavity through the gas outlet channel.
10. The formed element floating enrichment chip according to claim 1, characterized in that: The sample adding port is communicated with the upper cavity of the accommodating cavity through the sample adding channel; the gas outlet is communicated with the upper cavity of the accommodating cavity through the gas outlet channel.