96-hole PCR (Polymerase Chain Reaction) tube plate combined type filtering structure

Abstract

The utility model discloses a 96-hole PCR (Polymerase Chain Reaction) tube plate combined type filtering structure which comprises an upper-layer 96-hole PCR tube plate and a lower-layer 96-hole PCR tube plate, the upper-layer 96-hole PCR tube plate and the lower-layer 96-hole PCR tube plate are detachably connected through a connecting piece, and PCR tubes of the upper-layer 96-hole PCR tube plate and PCR tubes of the lower-layer 96-hole PCR tube plate are arranged in a one-to-one correspondence manner; an outlet is formed in the bottom end of each PCR tube of the upper-layer 96-hole PCR tube plate, and activated carbon filter paper is laid in each PCR tube of the upper-layer 96-hole PCR tube plate. The 96-hole PCR tube plate disclosed by the utility model has the advantages that the upper-layer 96-hole PCR tube plate and the lower-layer 96-hole PCR tube plate are combined for use, meanwhile, the bottom ends of the PCR tubes of the upper-layer 96-hole PCR tube plate are opened, and the activated carbon filter paper is arranged in the PCR tubes, so that precipitates in a sample are effectively filtered, dust and the like are ensured to fall into the lower-layer 96-hole PCR tube plate, a good sealing effect is achieved, and the service life of the 96-hole PCR tube plate is prolonged. And the service lives of a subsequent chromatographic column and a front-end pipeline are effectively ensured.

Description

Technical Field

[0001] The utility model relates to the technical field of filtering devices, in particular to a 96-well PCR tube-plate combined filtering structure. Background Art

[0002] Liquid chromatography-mass spectrometry technology combines the good separation performance of liquid chromatography with the advantages of high sensitivity and high selectivity of tandem mass spectrometry. It has become one of the most vital quantitative analysis technologies in the field of modern biomedicine. Its clinical application is very extensive, including the monitoring of hormones, vitamins, and drug concentrations. When using LC-MS technology to detect the substance to be tested, it is necessary to perform sample pretreatment operations through protein precipitation method, and absorb the supernatant to the 96-well PCR tube plate for testing. The 96-well PCR tube plate includes a plate body with 96 holes, and PCR tubes corresponding to the 96 holes. The PCR tube is fixedly connected to the 96-well plate body. When transferring the supernatant, due to improper pretreatment operations and various reasons such as dust falling, it is very easy to absorb precipitation, or foreign matter falls into the 96-well PCR tube plate, resulting in blockage of the chromatographic column and the front-end pipeline after the machine is installed, resulting in a great waste of time and personnel costs. In view of this, there is an urgent need for a 96-well PCR tube plate combined filtering structure that can filter and adsorb impurities and precipitation and is suitable for experiments. Utility Model Content

[0003] The utility model aims to provide a 96-well PCR tube-plate combined filtering structure.

[0004] The utility model is implemented by the following technical scheme: a 96-well PCR tube plate combined filtering structure, which includes an upper 96-well PCR tube plate and a lower 96-well PCR tube plate, the upper 96-well PCR tube plate and the lower 96-well PCR tube plate are detachably connected through a connecting piece, and the PCR tubes of the upper 96-well PCR tube plate and the PCR tubes of the lower 96-well PCR tube plate are arranged one by one;

[0005] An outlet is respectively opened at the bottom end of each PCR tube of the upper 96-well PCR tube plate, and activated carbon filter paper is respectively laid inside each PCR tube of the upper 96-well PCR tube plate.

[0006] Furthermore, the connecting piece is a buckle, and the buckle piece and the fastener of the buckle are fixedly connected to the outer walls of the upper 96-well PCR tube plate and the lower 96-well PCR tube plate respectively.

[0007] Furthermore, the bottom outlets of the PCR tubes of the upper 96-well PCR tube plate extend into the interior of the plate body of the lower 96-well PCR tube plate.

[0008] Furthermore, handles are fixed on both side walls of the upper 96-well PCR tube plate.

[0009] The utility model has the advantages of: through the combined use of the upper 96-well PCR tube plate and the lower 96-well PCR tube plate, the bottom end of the PCR tube of the upper 96-well PCR tube plate is opened, and activated carbon filter paper is arranged inside, which effectively filters the precipitate in the sample and reduces the precipitate from entering the subsequent detection link; at the same time, it ensures that dust and the like fall into the lower 96-well PCR tube plate, has a good sealing effect, effectively ensures the service life of the subsequent chromatographic column and the front-end pipeline, and with the cooperation of the activated carbon filter paper, does not require long-term precipitation, improves the processing speed of the supernatant, and ensures work efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0010] In order to more clearly illustrate the embodiments of the utility model or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the utility model. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0011] Figure 1 It is a schematic diagram of the structure of the utility model;

[0012] Figure 2 This is a schematic diagram of the structure of the upper 96-well PCR tube plate of the utility model;

[0013] Figure 3 This is a schematic diagram of the structure of the lower layer 96-well PCR tube plate of the utility model;

[0014] In the figure: an upper 96-well PCR tube plate 1, a lower 96-well PCR tube plate 2, a connector 3, a buckle 3.1, an outlet 4, an activated carbon filter paper 5, a handle 6, a PCR tube 7, and a plate body 8. DETAILED DESCRIPTION

[0015] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0016] like Figure 1-3As shown, the 96-well PCR tube plate combined filtering structure comprises an upper 96-well PCR tube plate 1 and a lower 96-well PCR tube plate 2, the upper 96-well PCR tube plate 1 and the lower 96-well PCR tube plate 2 are detachably connected via a connector 3, and the PCR tubes 7 of the upper 96-well PCR tube plate 1 and the PCR tubes 7 of the lower 96-well PCR tube plate 2 are arranged one by one;

[0017] Each PCR tube 7 of the upper 96-well PCR tube plate 1 is provided with an outlet 4 at the bottom end, and each PCR tube 7 of the upper 96-well PCR tube plate 1 is provided with an activated carbon filter paper 5 inside. The activated carbon filter paper 5 can fully contact with the adsorbed substance, so that the activated carbon can achieve the purpose of absorbing precipitation. The adsorption principle of activated carbon is physical adsorption, which does not change the structure of the substance to be tested, nor does it react chemically with it, and has no effect on the measurement of experimental results. It can absorb various impurities, precipitation and other foreign matter to a great extent, solves the problem of clogging the front-end pipeline and the chromatographic column, and ensures the accuracy of the measurement result; at the same time, there is no need to wait for the sample to settle, which saves the processing time.

[0018] The connecting piece 3 is a buckle 3.1, and the buckle 3.1 and the fastener of the buckle 3.1 are fixedly connected to the outer walls of the upper 96-well PCR tube plate 1 and the lower 96-well PCR tube plate 2 respectively, thereby increasing the sealing performance of the device. When the device is moved, the upper 96-well PCR tube plate 1 can effectively protect the lower 96-well PCR tube plate 2 and reduce the pollution problem.

[0019] The bottom outlet 4 of the PCR tube 7 of the upper 96-well PCR tube plate 1 extends to the inside of the plate body 8 of the lower 96-well PCR tube plate 2. The specific function is to guide the sample entering the upper 96-well PCR tube plate 1, and guide the filtered sample into the lower 96-well PCR tube plate 2, while ensuring the sealing of the lower 96-well PCR tube plate 2, and effectively reducing the external impurities directly entering the lower 96-well PCR tube plate 2.

[0020] Handles 6 are fixed to both side walls of the plate body 8 of the upper 96-well PCR tube plate 1, respectively, to facilitate movement and holding, and effectively ensure the stability of movement during transportation.

[0021] The specific operation process of this embodiment is as follows:

[0022] The upper 96-well PCR tube plate 1 and the lower 96-well PCR tube plate 2 are connected into a whole by means of buckles 3.1, and then the conical activated carbon filter paper 5 is placed into the PCR tube 7 of the upper 96-well PCR tube plate 1;

[0023] The samples are sequentially poured into the upper 96-well PCR tube plate 1, and the samples are filtered through the activated carbon filter paper 5 to filter out the precipitated impurities, etc. The treated samples are then placed into the lower 96-well PCR tube plate 2 for storage;

[0024] After all samples are placed on the lower 96-well PCR tube plate 2, the entire device is moved to the detector;

[0025] Open the buckle 3.1, remove the upper 96-well PCR tube plate 1 from the lower 96-well PCR tube plate 2, and then test the samples in the lower 96-well PCR tube plate 2.

[0026] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. 96-well PCR tube-plate combined filtration structure, characterized in that: It comprises an upper 96-well PCR tube plate and a lower 96-well PCR tube plate, wherein the upper 96-well PCR tube plate and the lower 96-well PCR tube plate are detachably connected via a connector, and the PCR tubes of the upper 96-well PCR tube plate and the PCR tubes of the lower 96-well PCR tube plate are arranged in one-to-one correspondence; An outlet is respectively opened at the bottom end of each PCR tube of the upper 96-well PCR tube plate, and activated carbon filter paper is respectively laid inside each PCR tube of the upper 96-well PCR tube plate.

2. The 96-well PCR tube-plate combined filtration structure according to claim 1, characterized in that: The connecting piece is a buckle, and the buckle piece and the fastener of the buckle are fixedly connected to the outer walls of the upper 96-well PCR tube plate and the lower 96-well PCR tube plate respectively.

3. The 96-well PCR tube-plate combined filtration structure according to claim 2, characterized in that: The bottom outlets of the PCR tubes of the upper 96-well PCR tube plate extend into the interior of the plate body of the lower 96-well PCR tube plate.

4. The 96-well PCR tube-plate combined filtration structure according to claim 3, characterized in that: Handles are fixed on both side walls of the upper 96-well PCR tube plate.

Images