Double-piston closed type pipetting device

Abstract

The utility model relates to a double-piston closed type pipetting device, and relates to the field of biological medicine. Accurate and quantitative positive pressure and negative pressure are achieved through the double pistons, and mixing and separation of different types of specimens in a small closed space can be achieved through the side flow channel technology in cooperation with the gravity effect, the siphoning effect, the molecular surface tension and other principles. In addition, according to the double-piston closed pipetting device, the reagent cavities can be accumulated according to different pipetting requirements, and the double-piston closed pipetting device is suitable for manual or automatic closed pipetting experiment requirements of complex processes.

Description

Technical Field

[0001] The present application relates to the field of biomedical technology, and in particular to a double-piston closed liquid transfer device. Background Art

[0002] With the development of science and technology, dropper pipettes and pipettes are widely used in various laboratories. As representatives of open pipetting, dropper pipettes and pipettes use the principle of air replacement to handle some toxic and harmful samples. They need to be operated in special equipment such as fume hoods and biosafety cabinets. The operation is cumbersome and prone to safety accidents. In addition, in the process of separating different types of samples, it is necessary to use various principles to operate with the help of centrifuges, pistons or vacuum pumps. In this process, the containers containing samples need to be labeled, opened and closed, etc., which is time-consuming and laborious.

[0003] In the related technologies, commercialized microfluidic methods can also realize closed liquid transfer processing, but the microfluidic method needs to be implemented through etching, surface treatment, bonding and other technologies with high production costs, which affects the widespread promotion and application of this technology. Utility Model Content

[0004] The purpose of the present application is to provide a double-piston closed pipetting device to solve the problems existing in the above-mentioned prior art.

[0005] To achieve the above purpose, the technical solution adopted in this application is:

[0006] A double-piston closed liquid transfer device, characterized in that it comprises a reagent box and an injection box located below the reagent box, wherein a reagent chamber partition is arranged inside the reagent box in a direction perpendicular to a horizontal plane, wherein the reagent chamber partition divides the interior of the injection box into a first reagent chamber and a second reagent chamber, wherein a first filter chamber and a second filter chamber are arranged at the bottom of the first reagent chamber and the second reagent chamber, respectively, wherein the first filter chamber and the second filter chamber are connected to the injection chamber in the injection box through a first through hole and a second through hole, respectively; a first injection rod piston and a second injection rod piston are sealingly connected in the injection chamber, wherein the first and second injection rod pistons ... The first injection rod piston and the second injection rod piston are respectively connected to the external drive motor through the first injection rod and the second injection rod; the first reagent chamber also has an injection hole passage connected to the injection chamber, one end of the injection hole passage is located at the top of the injection chamber, and the other end of the injection hole passage is located at the middle and upper part of the first reagent chamber, and the injection hole passage is located on the side of the first filter chamber close to the second filter chamber; the second reagent chamber also has a drainage hole passage connected to the injection chamber, one end of the drainage hole passage is located at the top of the injection chamber, and the other end of the drainage hole passage is located at the bottom of the second reagent chamber.

[0007] In a possible implementation, the bottoms of the first filter cavity and the second filter cavity are provided with anti-blocking protrusions that are evenly spaced and circumferentially distributed, and an anti-blocking filter element is mounted on the anti-blocking protrusions via a filter element fixing ring.

[0008] In a possible implementation, one end of the drainage hole passage located at the bottom of the second reagent chamber further comprises a filter element fixing column, and a drainage hole filter element is disposed in the filter element fixing column.

[0009] In a possible implementation, the top of the reagent box body is provided with a reagent box cover, and the reagent box cover is provided with air holes.

[0010] In a possible implementation, a breathable filter element fixing groove is provided on the top of the reagent chamber partition, a breathable filter element is provided in the breathable filter element fixing groove, and the position of the breathable filter element corresponds to the position of the breathable hole.

[0011] In a possible implementation, the reagent box cover is flexibly connected to the reagent box body.

[0012] In a possible implementation, the reagent box cover is flexibly connected to the reagent box body via a plurality of sets of flexible connection belts.

[0013] The beneficial effects of the technical solution provided by this application include at least:

[0014] This application uses a double piston to achieve accurate quantitative positive and negative pressure, and through the lateral flow channel technology, combined with the principles of gravity, siphon action, molecular surface tension, etc., it can achieve the mixing and separation of different types of specimens in a smaller closed space. In addition, the double piston closed pipetting device can accumulate reagent chambers according to different pipetting requirements, which is suitable for complex process manual or automated closed pipetting experimental requirements. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] The accompanying drawings are used to provide a further understanding of the present application and constitute a part of the specification. Together with the embodiments of the present application, they are used to explain the present application and do not constitute a limitation of the present application. In the accompanying drawings:

[0016] Figure 1 A schematic structural diagram of a double-piston closed liquid transfer device provided by an exemplary embodiment of the present application is shown;

[0017] Figure 2 An exploded schematic diagram of a double-piston closed liquid transfer device in a cross-sectional state provided by an exemplary embodiment of the present application is shown;

[0018] In the figure:

[0019] 1. Reagent box; 2. Injection box;

[0020] 11. Reagent chamber partition; 12. First reagent chamber; 13. Second reagent chamber; 14. First filter chamber; 15. Second filter chamber; 16. First through hole; 17. Second through hole; 18. Injection hole passage; 19. Drain hole passage; 110. Anti-blocking raised strip; 111. Filter element fixing ring; 112. Anti-blocking filter element; 113. Filter element fixing column; 114. Drain hole filter element; 115. Reagent box cover; 116. Air vent; 117. Air filter element fixing groove; 118. Air filter element; 119. Soft connecting belt;

[0021] 21. Injection chamber; 22. First injection rod piston; 23. Second injection rod piston; 24. First injection rod; 25. Second injection rod. DETAILED DESCRIPTION

[0022] The following will be combined with the drawings in the embodiments of the present application to clearly and completely describe the technical solutions in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, not all of the embodiments. Based on the embodiments in the present application, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of this application.

[0023] Among them, the same parts are represented by the same figure marks. It should be noted that the words "front", "rear", "left", "right", "up" and "down" used in the following description refer to the directions in the drawings of the present application specification, and the words "bottom surface" and "top surface", "inside" and "outside" refer to the direction towards or away from a specific component, respectively. In addition, the terms "first" and "second" are used for descriptive purposes only and cannot be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Therefore, the features defined as "first" and "second" may explicitly or implicitly include one or more of the features. In the description of the present application specification, the meaning of "multiple" is two or more.

[0024] The present application is further described below in conjunction with the accompanying drawings and embodiments.

[0025] Figure 1 A schematic structural diagram of a double-piston closed liquid transfer device provided by an exemplary embodiment of the present application is shown; Figure 2 FIG. 1 is an exploded schematic diagram of a double-piston closed pipetting device provided by an exemplary embodiment of the present application in a cross-sectional state. Figure 1 and Figure 2From the perspective of the double-piston closed pipetting device, the double-piston closed pipetting device includes a reagent box 1 and an injection box 2 located below the reagent box 1. A reagent chamber partition 11 is arranged inside the reagent box 1 in a direction perpendicular to the horizontal plane. The reagent chamber partition 11 divides the interior of the injection box 2 into a first reagent chamber 12 and a second reagent chamber 13. The bottoms of the first reagent chamber 12 and the second reagent chamber 13 are respectively provided with a first filter chamber 14 and a second filter chamber 15. The first filter chamber 14 and the second filter chamber 15 are respectively connected to the injection chamber 21 in the injection box 2 through a first through hole 16 and a second through hole 17.

[0026] Specifically, combined Figure 1 and Figure 2 From the perspective of the embodiment, the injection chamber 21 is sealed and connected with the first injection rod piston 22 and the second injection rod piston 23, and the first injection rod piston 22 and the second injection rod piston 23 are respectively connected to the external drive motor through the first injection rod 24 and the second injection rod 25; the first reagent chamber 12 also has an injection hole passage 18 connected to the injection chamber 21, one end of the injection hole passage 18 is located at the top of the injection chamber 21, and the other end of the injection hole passage 18 is located in the middle and upper part of the first reagent chamber 12, and the injection hole passage 18 is located on the side of the first filter chamber 14 close to the second filter chamber 15; the second reagent chamber 13 also has a drainage hole passage 19 connected to the injection chamber 21, one end of the drainage hole passage 19 is located at the top of the injection chamber 21, and the other end of the drainage hole passage 19 is located at the bottom of the second reagent chamber 13.

[0027] In the embodiment of the present application, the first injection rod 24 and the second injection rod 25 are driven by an external driving motor, thereby driving the first injection rod piston 22 and the second injection rod piston 23 to slide in the injection cavity 21. The first injection rod piston 22 and the second injection rod piston 23 are interference-fitted in the injection cavity 21, and directional pipetting and precise control of the pipetting volume are achieved by controlling the relative spacing and the dwelling position of the double pistons.

[0028] In the embodiment of the present application, the first injection rod piston 22 and the second injection rod piston 23 have a multiple thread design so as to reduce the movement resistance as much as possible while ensuring sealing, thereby ensuring smooth injection or suction action and accurate pipetting.

[0029] In the embodiment of the present application, the first injection rod piston 22 and the second injection rod piston 23 also have a card groove and a chamfered smooth structure for relatively clamping the first injection rod 24 and the second injection rod 25, ensuring that the injection rod pistons are relatively difficult to fall off; and during the production process, the first injection rod piston 22 and the second injection rod piston 23 can seal the first through hole 16 and the second through hole 17 under the first reagent chamber 12 and the second reagent chamber 13 to prevent volatile or toxic reagents such as alcohol and aldehyde from volatilizing or leaking during storage and transportation.

[0030] For further information, see Figure 2 The bottom of the first filter chamber 14 and the second filter chamber 15 are provided with anti-blocking protrusions 110 evenly spaced and circumferentially distributed, and an anti-blocking filter element 112 is installed on the anti-blocking protrusions 110 through a filter element fixing ring 111. The end of the drainage hole passage 19 located at the bottom of the second reagent chamber 13 is also provided with a filter element fixing column 113, and a drainage hole filter element 114 is provided in the filter element fixing column 113.

[0031] In the embodiment of the present application, the drainage hole filter 114 is used to separate different components in the reagent.

[0032] For further information, see Figure 2 The top of the reagent box body 1 is provided with a reagent box cover 115, and the reagent box cover 115 is provided with an air hole 116. The top of the reagent chamber partition 11 is provided with an air filter element fixing groove 117, and the air filter element fixing groove 117 is provided with an air filter element 118, and the position of the air filter element 118 corresponds to the position of the air hole 116. The reagent box cover 115 is flexibly connected to the reagent box body 1.

[0033] In the embodiment of the present application, the reagent chamber can be isolated from the outside while maintaining air pressure balance through the air permeable filter element 118.

[0034] In some embodiments, see Figure 2 The reagent box cover 115 is flexibly connected to the reagent box body 1 through multiple sets of soft connection belts 119.

[0035] Next, combine Figure 2 , the working principle of a double-piston closed pipetting device involved in an embodiment of the present application is explained.

[0036] Add reagent A and reagent B into the first reagent chamber 12 and the second reagent chamber 13 respectively, cover the reagent box cover 115 and make sure it is tightly sealed;

[0037] Drive the first injection rod 24 and the second injection rod 25 so that the first injection rod piston 22 is located on the left side below the first through hole 16 and the second injection rod piston 23 is located on the right side below the first through hole 16;

[0038] The first injection rod 24 is driven to move the first injection rod piston 22 to the left, so as to suck the reagent A into the cavity of the injection cavity 21;

[0039] The second injection rod 25 is driven to move the second injection rod piston 23 to the right until the second injection rod piston 23 is located on the right side below the injection hole passage 18 and remains in position;

[0040] The first injection rod 24 is driven to move the first injection rod piston 22 to the right, and the reagent A is injected into the second reagent chamber 13 through the injection hole passage 18;

[0041] The first injection rod 24 is repeatedly driven to move the first injection rod piston 22 slightly to the left and right to fully mix the reagent A and the reagent B;

[0042] The second injection rod 25 is driven to move the second injection rod piston 23 to the right until the second injection rod piston 23 is located on the right side below the drainage hole passage 19 and keeps the position unchanged;

[0043] The first injection rod 24 is driven to move the first injection rod piston 22 to the right, so that the first injection rod piston 22 is located between the drainage hole passage 19 and the second through hole 17;

[0044] The first injection rod 24 is driven to move the first injection rod piston 22 to the left, so as to suck the mixed solution of reagent A and reagent B into the cavity of the injection cavity 21;

[0045] Drive the first injection rod 24 and the second injection rod 25 so that the first injection rod piston 22 is located on the left side below the first through hole 16 and the second injection rod piston 23 is located on the right side below the first through hole 16;

[0046] The first injection rod 24 is driven to move the first injection rod piston 22 to the right, and the mixed solution of reagent A and reagent B is injected into the first reagent chamber 12 through the first through hole 16;

[0047] Drive the first injection rod 24 and the second injection rod 25 so that the first injection rod piston 22 is located on the left side below the second through hole 17 and the second injection rod piston 23 is located on the right side below the second through hole 17;

[0048] The first injection rod 24 is driven to make the first injection rod piston 22 move to the left repeatedly by a small distance for multiple times, so as to fully mix the residual substances of the reaction of the mixed solution of reagent A and reagent B;

[0049] The first injection rod 24 is driven to move the first injection rod piston 22 to the left, so that the remaining substances from the reaction of the mixed solution of reagent A and reagent B are sucked into the cavity of the injection cavity 21 for standby use or transferred to other places.

[0050] In summary, the present application realizes accurate quantitative positive and negative pressure through dual pistons, and can realize mixing and separation of different types of specimens in a smaller closed space through lateral flow channel technology, combined with gravity, siphon action, molecular surface tension and other principles. In addition, the dual-piston closed liquid transfer device can accumulate reagent chambers according to different liquid transfer requirements, and is suitable for complex process manual or automated closed liquid transfer experiment requirements.

[0051] In the embodiments disclosed in the present application, the terms "installed", "connected", "connected", "fixed" and the like should be understood in a broad sense. For example, "connected" can be a fixed connection, a detachable connection, or an integral connection; "connected" can be a direct connection or an indirect connection through an intermediate medium. For ordinary technicians in this field, the specific meanings of the above terms in the embodiments disclosed in the present utility model can be understood according to specific circumstances.

[0052] The above is only a preferred implementation of the present application. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present application. These improvements and modifications should also be regarded as the scope of protection of the present application.

Claims

1. A double-piston closed liquid transfer device, characterized in that: The invention comprises a reagent box body and an injection box body located below the reagent box body, wherein a reagent chamber partition is arranged inside the reagent box body along a direction perpendicular to a horizontal plane, wherein the reagent chamber partition divides the inside of the injection box body into a first reagent chamber and a second reagent chamber, wherein a first filter chamber and a second filter chamber are arranged at the bottom of the first reagent chamber and the second reagent chamber, respectively, and the first filter chamber and the second filter chamber are connected to the injection chamber in the injection box body through a first through hole and a second through hole, respectively; A first injection rod piston and a second injection rod piston are sealingly connected in the injection cavity, and the first injection rod piston and the second injection rod piston are respectively connected to an external drive motor through the first injection rod and the second injection rod; The first reagent chamber also has an injection hole passage connected to the injection chamber, one end of the injection hole passage is located at the top of the injection chamber, the other end of the injection hole passage is located at the middle and upper part of the first reagent chamber, and the injection hole passage is located on a side of the first filter chamber close to the second filter chamber; The second reagent chamber also has a drainage hole passage communicating with the injection chamber, one end of the drainage hole passage is located at the top of the injection chamber, and the other end of the drainage hole passage is located at the bottom of the second reagent chamber.

2. The double-piston closed liquid transfer device according to claim 1, characterized in that: The bottoms of the first filter cavity and the second filter cavity are provided with anti-blocking protrusion strips which are evenly spaced and distributed circumferentially, and an anti-blocking filter element is installed on the anti-blocking protrusion strips via a filter element fixing ring.

3. The double-piston closed pipetting device according to claim 1, characterized in that: The end of the drainage hole passage located at the bottom of the second reagent chamber also has a filter element fixing column, and the filter element fixing column has a drainage hole filter element.

4. The double-piston closed pipetting device according to claim 1, characterized in that: The top of the reagent box body is provided with a reagent box cover, and the reagent box cover is provided with air holes.

5. The double-piston closed liquid transfer device according to claim 4, characterized in that: A breathable filter element fixing groove is provided on the top of the reagent chamber partition, a breathable filter element is provided in the breathable filter element fixing groove, and the position of the breathable filter element corresponds to the position of the breathable hole.

6. The double-piston closed liquid transfer device according to claim 4, characterized in that: The reagent box cover is flexibly connected to the reagent box body.

7. The double-piston closed pipetting device according to claim 6, characterized in that: The reagent box cover is flexibly connected to the reagent box body through multiple groups of soft connection belts.

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