Single cell screening, arranging, collecting and detecting device

Abstract

The utility model relates to the technical field of cell detection devices, and discloses a single cell screening, arranging, collecting and detecting device which comprises a collecting box, a bearing assembly, a rotating assembly and a liquid adding assembly, a box cover is installed on the top of the collecting box in a threaded mode, and a detection opening is formed in one side of the box cover. The bearing assembly comprises a supporting base, a bearing disc and a culture dish, the rotating assembly comprises a gear, a gear ring and a positioning assembly, the gear is rotationally installed on the side face of the collecting box, the gear ring is fixedly installed on the circumference of the bearing disc in a sleeving mode, the gear is meshed with the gear ring, and the positioning assembly is installed on the side, close to the gear, of the collecting box. The liquid adding assembly comprises a liquid storage tank, a piston barrel, an air cylinder, a piston plate, a liquid suction pipe and a liquid outlet pipe. By arranging the bearing assembly, the rotating assembly and the liquid adding assembly, the cell detection efficiency can be improved.

Description

Technical Field

[0001] The utility model relates to the technical field of cell detection devices, in particular to a single cell screening, arranging, collecting and detecting device. Background Art

[0002] Single-cell suspension is the basis for in vitro cell culture and flow cytometric analysis. The current traditional way to collect single-cell suspension is to filter tissue cells through a cell sieve to obtain a single-cell suspension. However, the disadvantage of the cell sieve is that the filtration area is too small and is not suitable for large-scale processing of tissue cell mixtures. In addition, it is difficult for operators to control the separation time, which can easily cause the effective ingredients of the single-cell suspension sample to be inactivated during the collection process.

[0003] After searching, Chinese patent publication number CN214881470U discloses a cell collection device. Although the patent can ensure the activity of single cell suspension, cell culture needs to be added after the single cells are collected to ensure the activity of the cells. Most of the existing single cell collection and detection devices require manual addition of cell culture fluid, and when detecting the collected single cells, the single cells need to be taken out first and then tested, and then put back into the collection device after testing. The process is complicated and affects the detection efficiency.

[0004] Therefore, how to design a single-cell screening, arranging, collecting and detecting device has become the problem we need to solve at present. Utility Model Content

[0005] 1. Technical issues to be solved

[0006] In view of the deficiencies of the prior art, the present invention provides a single cell screening, arranging, collecting and detecting device to solve the problems mentioned in the above background technology.

[0007] (II) Technical solution

[0008] To achieve the above-mentioned purpose, the utility model provides the following technical solutions: a single cell screening, arranging, collecting and detecting device, comprising a collecting box, a bearing assembly, a rotating assembly and a liquid adding assembly, wherein a box cover is threadedly installed on the top of the collecting box, a detection port is provided on one side of the box cover, the bearing assembly comprises a supporting seat, a bearing plate and a culture dish, the supporting seat is fixedly installed in the collecting box, the bearing plate is rotatably installed on the supporting seat, a plurality of annular placement grooves are provided on the upper surface of the bearing plate, the culture dish is placed in the placement groove, a clamping groove is provided on one side of the culture dish, and a clamping assembly is provided on one side of the placement groove;

[0009] The rotating assembly includes a gear, a gear ring and a positioning assembly, wherein the gear is rotatably mounted on the side of the collecting box, the gear ring is fixedly sleeved on the circumference of the carrying plate, and the gear is meshed with the gear ring, and the positioning assembly is mounted on a side of the collecting box close to the gear;

[0010] The liquid adding assembly includes a liquid storage box, a piston barrel, a cylinder, a piston plate, a pipette and a liquid outlet pipette. The liquid storage box is installed in a collecting box, the piston barrel is installed on the inner wall of the collecting box, the cylinder is fixedly installed inside the piston barrel, the piston plate is fixedly connected to the lower end of the cylinder, the pipette and the liquid outlet pipette are both fixedly connected to the bottom of the piston barrel, the pipette extends into the liquid storage box, and the liquid outlet pipe extends to above the culture dish.

[0011] Preferably, a sealing cover is threadedly inserted into the top of the detection port.

[0012] Preferably, the clamping assembly includes a first spring and a clamping block, wherein the first spring is embedded in the inner wall of the placement slot, the clamping block abuts against one end of the first spring, and a push handle penetrating the carrier plate is provided on the top of the clamping block.

[0013] Preferably, a handle is fixedly connected to a side of the culture dish away from the slot.

[0014] Preferably, the positioning assembly includes a sleeve, a second spring, a positioning block and a pressing block, the sleeve is fixedly connected to the side wall of the collection box, the second spring is arranged in the sleeve, the positioning block and the pressing block are respectively abutted against two ends of the second spring, a positioning groove is provided at one end of the gear, pulleys are rotatably installed on both sides of the pressing block inside the sleeve, a connecting rope is overlapped on the pulley, and the two ends of the connecting rope are respectively connected to the positioning block and the pressing block.

[0015] Preferably, one-way valves are installed on the ends of the liquid suction pipe and the liquid discharge pipe close to the piston cylinder.

[0016] (III) Beneficial effects

[0017] The utility model provides a single cell screening, arranging, collecting and detecting device, which has the following beneficial effects:

[0018] First, by setting a bearing assembly and a rotating assembly, multiple culture dishes can be placed one by one in the corresponding placement slots, and the culture dishes are supported by the bearing plate. During the inspection, the operator can turn the gear to rotate, so that the gear drives the gear ring to rotate, and the gear ring drives the bearing plate to rotate, so that different culture dishes can be rotated one by one to the bottom of the inspection port for inspection;

[0019] Second, by setting a positioning component, when the gear rotates one circle each time, the positioning block will be aligned with the positioning groove. At this time, the second spring will push the positioning block to insert into the positioning groove to fix the gear, thereby indirectly positioning the carrier plate, so that the culture dish to be tested is kept below the detection port, avoiding the shaking of the culture dish and affecting the detection effect;

[0020] Thirdly, by setting up a liquid adding component, when the carrier plate rotates, the culture dishes will rotate one by one to the bottom of the liquid outlet pipe. At this time, the cylinder can be activated to extend and retract, driving the piston plate to slide back and forth in the piston cylinder, and the culture liquid in the liquid storage tank is sucked into the piston cylinder through the liquid pipette, and then added to the culture dish through the liquid outlet pipe. There is no need for the staff to take out the culture dishes one by one and manually add nutrient solution, which saves manpower and improves the detection efficiency.

[0021] Fourthly, by setting up the card-joint assembly, the culture dish can be fixed. When disassembling the culture dish, you only need to push the push handle so that the push handle drives the card block to move out of the card slot, and then you can use the handle to take out the culture dish, which is convenient for taking and placing the culture dish so that the culture dish can be cleaned and replaced. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 It is a schematic diagram of the overall structure of the utility model;

[0023] Figure 2 This is a schematic diagram of the internal structure of the utility model;

[0024] Figure 3 It is a structural schematic diagram of the carrier plate of the utility model;

[0025] Figure 4 For this utility model Figure 2 A magnified view of the structure at center;

[0026] Figure 5 For this utility model Figure 2 Enlarged view of the structure at point B in the middle.

[0027] In the figure: 1. collecting box; 11. box cover; 12. detection port; 13. sealing cover; 2. bearing assembly; 21. supporting seat; 22. bearing plate; 23. culture dish; 24. placement slot; 25. card slot; 26. card assembly; 261. first spring; 262. card block; 263. push handle; 27. handle; 3. rotating assembly; 31. gear; 32. gear ring; 33. positioning assembly; 331. sleeve; 332. second spring; 333. positioning block; 334. pressing block; 335. positioning slot; 336. pulley; 337. connecting rope; 4. liquid adding assembly; 41. liquid storage tank; 42. piston cylinder; 43. cylinder; 44. piston plate; 45. pipette; 46. liquid outlet pipette. DETAILED DESCRIPTION

[0028] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0029] In the description of the present invention, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise" and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the referred device or element must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as a limitation on the present invention.

[0030] In addition, the terms "first" and "second" are used for descriptive purposes only and cannot be understood as indicating or suggesting relative importance or implicitly indicating the number of technical features indicated. Therefore, the features defined as "first" and "second" may explicitly or implicitly include one or more of such features. In the description of the present utility model, "multiple" means two or more, unless otherwise clearly and specifically defined.

[0031] like Figure 1-5 As shown, the utility model provides a technical solution: a single cell screening, arranging, collecting and detecting device, comprising a collecting box 1, a bearing assembly 2, a rotating assembly 3 and a liquid adding assembly 4, a box cover 11 is threadedly installed on the top of the collecting box 1, a detecting port 12 is opened on one side of the box cover 11, a sealing cover 13 is threadedly inserted on the top of the detecting port 12, the detecting port 12 can be sealed by setting the sealing cover 13, and during detection, the sealing cover 13 can be opened and a microscope can be aligned with the detecting port 12 for detection.

[0032] Reference Figure 2 and Figure 3 The carrying component 2 includes a supporting seat 21, a carrying plate 22 and a culture dish 23. The supporting seat 21 is fixedly installed in the collecting box 1, and the carrying plate 22 is rotatably installed on the supporting seat 21. The upper surface of the carrying plate 22 is provided with a plurality of annular placement grooves 24, and the culture dish 23 is placed in the placement grooves 24. By setting the carrying component 2, a plurality of culture dishes 23 can be placed one by one in the corresponding placement grooves 24, and the culture dishes 23 are supported by the carrying plate 22.

[0033] Specifically, a card slot 25 is provided on one side of the culture dish 23, and a card connection assembly 26 is provided on one side of the placement groove 24. The card connection assembly 26 includes a first spring 261 and a card block 262. The first spring 261 is embedded and installed on the inner wall of the placement groove 24, and the card block 262 abuts against one end of the first spring 261. The top of the card block 262 is provided with a push handle 263 that passes through the carrier plate 22; a handle 27 is fixedly connected to the side of the culture dish 23 away from the card slot 25. By setting the card connection assembly 26, the culture dish 23 can be fixed. When disassembling the culture dish 23, it is only necessary to push the push handle 263 so that the push handle 263 drives the card block 262 to move out of the card slot 25, and the culture dish 23 can be taken out by using the handle 27, which facilitates the placement and removal of the culture dish 23 so as to clean and replace the culture dish 23.

[0034] Reference Figure 2 and Figure 5 The rotating assembly 3 includes a gear 31, a gear ring 32 and a positioning assembly 33. The gear 31 is rotatably mounted on the side of the collecting box 1, and the gear ring 32 is fixedly mounted on the circumference of the carrying plate 22, and the gear 31 is meshed with the gear ring 32. For each rotation of the gear 31, the corresponding gear ring 32 rotates exactly 60°. The positioning assembly 33 is mounted on a side of the collecting box 1 close to the gear 31. By setting the rotating assembly 3, during detection, the operator can rotate the gear 31 to make the gear 31 drive the gear ring 32 to rotate, and the gear ring 32 drives the carrying plate 22 to rotate, so that different culture dishes 23 can be rotated one by one to the bottom of the detection port 12 for detection.

[0035] Specifically, the positioning assembly 33 includes a sleeve 331, a second spring 332, a positioning block 333 and a pressing block 334. The sleeve 331 is fixedly connected to the side wall of the collection box 1, the second spring 332 is arranged in the sleeve 331, the positioning block 333 and the pressing block 334 are respectively abutted against the two ends of the second spring 332, a positioning groove 335 is opened at one end of the gear 31, and pulleys 336 are rotatably installed on both sides of the pressing block 334 inside the sleeve 331, and a connecting rope 337 is overlapped on the pulley 336. The two ends of the connecting rope 337 are respectively connected to the positioning block 333 and the pressing block 334, through A positioning assembly 33 is provided. When the gear 31 rotates one circle each time, the positioning block 333 will be aligned with the positioning slot 335. At this time, the second spring 332 will push the positioning block 333 to be inserted into the positioning slot 335 to fix the gear 31, thereby indirectly positioning the carrier plate 22, so that the culture dish 23 to be tested is kept below the detection port 12 to prevent the culture dish 23 from shaking and affecting the detection effect; when it is necessary to rotate the gear 31, it is only necessary to press the pressing block 334, and pull the positioning block 333 out of the positioning slot 335 through the connecting rope 337 to release the positioning, which is very convenient to operate.

[0036] Reference Figure 2The liquid adding assembly 4 includes a liquid storage box 41, a piston barrel 42, a cylinder 43, a piston plate 44, a pipette 45 and a liquid outlet pipette 46. The liquid storage box 41 is installed in the collection box 1. The liquid storage box 41 is filled with culture fluid. The piston barrel 42 is installed on the inner wall of the collection box 1. The cylinder 43 is fixedly installed inside the piston barrel 42. The piston plate 44 is fixedly connected to the lower end of the cylinder 43. The pipette 45 and the liquid outlet pipette 46 are both fixedly connected to the bottom of the piston barrel 42. The pipette 45 extends into the liquid storage box 41, and the liquid outlet pipette 46 extends to the top of the culture dish 23. 5 and one end of the liquid outlet pipe 46 close to the piston cylinder 42 are both equipped with a one-way valve. By setting the liquid adding component 4, when the carrier plate 22 rotates, the culture dishes 23 will rotate one by one to the bottom of the liquid outlet pipe 46. At this time, the cylinder 43 can be started to extend and retract, driving the piston plate 44 to slide back and forth in the piston cylinder 42, and the culture liquid in the liquid storage tank 41 is sucked into the piston cylinder 42 through the liquid pipette 45, and then added to the culture dishes 23 through the liquid outlet pipe 46. There is no need for the staff to take out the culture dishes 23 one by one and manually add nutrient solution, which saves manpower and improves the detection efficiency.

[0037] The working process of the utility model is as follows: by setting the bearing component 2, a plurality of culture dishes 23 can be placed one by one in the corresponding placement groove 24, and the culture dishes 23 are supported by the bearing plate 22. By setting the rotating component 3, during detection, the operator can turn the gear 31 to rotate, so that the gear 31 drives the gear ring 32 to rotate, and the different culture dishes 23 can be rotated one by one to the bottom of the detection port 12 for detection. During detection, the sealing cover 13 can be opened, and the microscope can be aligned with the detection port 12 for detection; in addition, by setting the liquid adding component 4, when the bearing plate 22 rotates, the culture dishes 23 will be rotated one by one to the bottom of the liquid outlet pipe 46. At this time, the cylinder 43 can be started to extend and retract, driving the piston plate 44 to slide back and forth in the piston cylinder 42, and the culture solution in the liquid storage tank 41 is sucked into the piston cylinder 42 through the pipette 45, and then added to the culture dish 23 through the liquid outlet pipe 46. There is no need for the staff to take out the culture dishes 23 one by one and manually add nutrient solution, which saves manpower and improves detection efficiency.

[0038] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device.

[0039] Although the embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A single cell screening, arranging, collecting and detecting device, comprising a collecting box (1), a carrying component (2), a rotating component (3) and a liquid adding component (4), characterized in that: The top of the collection box (1) is threadedly mounted with a box cover (11), one side of the box cover (11) is provided with a detection port (12), the bearing assembly (2) comprises a support seat (21), a bearing plate (22) and a culture dish (23), the support seat (21) is fixedly mounted in the collection box (1), the bearing plate (22) is rotatably mounted on the support seat (21), a plurality of annular placement grooves (24) are provided on the upper surface of the bearing plate (22), the culture dish (23) is placed in the placement groove (24), a clamping groove (25) is provided on one side of the culture dish (23), and a clamping assembly (26) is provided on one side of the placement groove (24); The rotating assembly (3) comprises a gear (31), a gear ring (32) and a positioning assembly (33); the gear (31) is rotatably mounted on a side of the collecting box (1); the gear ring (32) is fixedly sleeved on the circumference of the carrying plate (22), and the gear (31) is meshed with the gear ring (32); and the positioning assembly (33) is mounted on a side of the collecting box (1) close to the gear (31); The liquid adding assembly (4) comprises a liquid storage box (41), a piston cylinder (42), a cylinder (43), a piston plate (44), a liquid pipette (45) and a liquid outlet pipette (46); the liquid storage box (41) is installed in the collection box (1); the piston cylinder (42) is installed on the inner wall of the collection box (1); the cylinder (43) is fixedly installed inside the piston cylinder (42); the piston plate (44) is fixedly connected to the lower end of the cylinder (43); the liquid pipette (45) and the liquid outlet pipette (46) are both fixedly connected to the bottom of the piston cylinder (42); the liquid pipette (45) extends into the liquid storage box (41); and the liquid outlet pipette (46) extends to the top of the culture dish (23).

2. A single cell screening, arranging, collecting and detecting device according to claim 1, characterized in that: A sealing cover (13) is threadedly inserted into the top of the detection port (12).

3. The single cell screening, arranging, collecting and detecting device according to claim 1, characterized in that: The clamping assembly (26) comprises a first spring (261) and a clamping block (262); the first spring (261) is embedded and installed in the inner wall of the placement groove (24); the clamping block (262) abuts against one end of the first spring (261); and a push handle (263) penetrating the carrier plate (22) is provided on the top of the clamping block (262).

4. The single cell screening, arranging, collecting and detecting device according to claim 1, characterized in that: A handle (27) is fixedly connected to a side of the culture dish (23) away from the slot (25).

5. The single cell screening, arranging, collecting and detecting device according to claim 1, characterized in that: The positioning assembly (33) comprises a sleeve (331), a second spring (332), a positioning block (333) and a pressing block (334); the sleeve (331) is fixedly connected to the side wall of the collection box (1); the second spring (332) is arranged in the sleeve (331); the positioning block (333) and the pressing block (334) are respectively abutted against two ends of the second spring (332); a positioning groove (335) is provided at one end of the gear (31); pulleys (336) are rotatably installed on both sides of the pressing block (334) inside the sleeve (331); a connecting rope (337) is overlapped on the pulley (336); and the two ends of the connecting rope (337) are respectively connected to the positioning block (333) and the pressing block (334).

6. The single cell screening, arranging, collecting and detecting device according to claim 1, characterized in that: One end of the liquid suction pipe (45) and the liquid outlet pipe (46) close to the piston cylinder (42) is equipped with a one-way valve.

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