Rapid sampling device for environment DNA (deoxyribonucleic acid)

Abstract

The utility model discloses an environmental DNA quick sampling device, which relates to the technical field of water body environmental DNA sampling, and comprises a sampling box, one side of the surface of the sampling box is fixedly connected with a support plate, the top surface of the support plate is fixedly connected with a water suction pump, the water outlet end of the water suction pump is fixedly connected with one side of the sampling box, and the water outlet end of the water suction pump is fixedly connected with the other side of the sampling box. The water inlet end of the water suction pump is fixedly connected with a water inlet pipe, and one end of the water inlet pipe is provided with a filtering assembly which is used for filtering impurities in a water body when the water suction pump pumps samples so as to prevent the impurities from blocking the water suction pump. Suction force is provided through the water suction pump, then the sampling assembly is used for sampling a water body, and the sampled water body is filtered through the filtering assembly to block impurities in the water, so that the problem that the impurities in the water enter the water suction pump during sampling, the water pump is blocked, and the sampling speed of the water suction pump is influenced is solved; meanwhile, the filtering assembly can be quickly disassembled and cleaned by utilizing the limiting assembly and the auxiliary assembly.

Description

Technical Field

[0001] The utility model relates to the technical field of water body environmental DNA sampling, in particular to an environmental DNA rapid sampling device. Background Art

[0002] The earliest application of environmental DNA was to directly extract DNA genomes from soil and sediments to study the diversity of uncultivable microorganisms and species identification. In recent years, it has gradually expanded from microbiology to the fields of animal and plant research, and has been well applied in the research of amphibians, fish, insect larvae, crustaceans and mammals. In aquatic environments, the use of environmental DNA technology can be well used to determine the distribution of species and analyze the community structure of species. Especially when the biological density of the target species is low, environmental DNA technology can well capture the DNA of the target species for analysis and research. Such a large-scale sequencing and analysis technology is much more efficient than the number of samples we obtain using conventional fishing methods.

[0003] After searching, the applicant found that a Chinese patent disclosed "an environmental DNA collection device for water bodies", and its publication number is "CN217059521U". This patent mainly places the DNA collection device on the water surface, and then the first blade rotates under the action of the water flow. The speed sensor senses the flow rate of the water body with the help of the transmission shaft and the first blade, and transmits the flow rate signal to the controller. The controller makes a judgment. When the flow rate meets the set value in the controller, the floating platform stops moving and then samples. This solves the problem of inaccurate water environmental DNA monitoring caused by the fluidity of the water body when sampling in the water body in the related technology;

[0004] The above-mentioned device can achieve more accurate DNA monitoring results in the sampling room by setting up a series of structures. However, in actual use, it samples the water body through a water pump. During sampling, due to the presence of a large amount of debris in the water, the water pump is easily blocked by the debris, thereby affecting the water output of the water pump, which directly affects the sampling speed. Utility Model Content

[0005] The purpose of the utility model is to provide an environmental DNA rapid sampling device to solve the problem proposed in the above-mentioned background technology that when sampling water bodies through a water pump in actual use, due to the presence of a large amount of debris in the water during sampling, the water pump is easily blocked by the debris during sampling, thereby affecting the water output of the water pump and directly affecting the sampling speed.

[0006] To achieve the above-mentioned purpose, the utility model provides the following technical solutions: an environmental DNA rapid sampling device, comprising a sampling box, a support plate fixedly connected to one side of the sampling box surface, a water pump fixedly connected to the top surface of the support plate, a water outlet end of the water pump fixedly connected to one side of the sampling box, a water inlet end of the water pump fixedly connected to a water inlet pipe, one end of the water inlet pipe is provided with a filter assembly for filtering impurities in the water body when the water pump draws samples to prevent the impurities from clogging the water pump, a limit assembly is provided inside the filter assembly for facilitating the release of the limit of the filter assembly for disassembly and cleaning, an auxiliary assembly is provided inside the filter assembly for ejecting the filter assembly after the limit assembly releases the limit of the filter assembly, and a sampling assembly is provided at the bottom of the filter assembly for sampling the water body through a water pump.

[0007] Preferably, the filter assembly includes a filter box, one side of the surface of the filter box is fixedly connected to one side of the surface of the sampling box, sliding grooves are provided on both sides of the inner wall of the filter box, the inner walls of the two sliding grooves are slidably connected to a filter plate, a water inlet is provided at the center of the surface of the filter plate and the filter box, a filter net is fixedly provided on the inner wall of the water inlet on the filter plate, and a baffle is fixedly connected to one end of the filter plate.

[0008] Preferably, the limit assembly includes two pairs of limit grooves opened on both sides of the filter plate surface, the inner walls of each pair of the limit grooves are slidably connected to the limit blocks, the opposite sides of the two limit blocks are fixedly connected to a pair of connecting rods, and the rod walls of each pair of the connecting rods are sleeved with a first spring, and the opposite ends of the two pairs of connecting rods pass through the opposite sides of the two sliding grooves and extend to both sides of the filter box surface.

[0009] Preferably, the limiting assembly further comprises two fixing plates, opposite sides of the two fixing plates are respectively fixedly connected to opposite ends of the two pairs of connecting rods, and opposite sides of the two fixing plates are fixedly connected with handles.

[0010] Preferably, the auxiliary component includes an auxiliary groove opened inside the filter box, two second springs are fixedly connected to one side of the inner wall of the auxiliary groove, one end of the two second springs is commonly fixedly connected to an auxiliary plate, and the side of the auxiliary plate away from the second spring is in contact with one side of the filter plate.

[0011] Preferably, the sampling assembly includes a hose, one end of which is fixedly connected to the bottom surface of the filter box and communicates with the water inlet, the other end of the hose is fixedly connected to a sampling tube, and the end of the sampling tube away from the hose is fixedly connected to a sampling head.

[0012] Preferably, two fixing clips are fixedly connected to one side of the surface of the sampling box.

[0013] Preferably, the auxiliary groove and the sliding groove are in the same plane.

[0014] The technical effects and advantages of the utility model are as follows: the utility model provides suction through a water pump, and then uses a sampling component to sample the water body, and the sampled water body will first be filtered through a filtering component to block the debris in the water, to prevent the debris in the water from entering the water pump during sampling, causing the water pump to be blocked and affecting the sampling speed of the water pump, and at the same time, the filtering component can be quickly disassembled and cleaned by using a limit component and an auxiliary component. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 It is a three-dimensional structural schematic diagram of the utility model.

[0016] Figure 2 It is a schematic diagram of the top view of the filter box of the utility model.

[0017] Figure 3 It is a three-dimensional structural schematic diagram of the filter plate of the utility model.

[0018] Figure 4 It is a front view cross-sectional structural schematic diagram of the filter box of the present utility model.

[0019] In the figure: 1. sampling box; 2. sampling component; 3. filtering component; 4. limiting component; 5. auxiliary component; 6. supporting plate; 7. water pump; 8. water inlet pipe; 9. fixing clamp; 201. hose; 202. sampling tube; 203. sampling head; 301. filtering box; 302. sliding groove; 303. filtering plate; 304. water inlet; 305. filtering net; 306. baffle; 401. limiting groove; 402. limiting block; 403. first spring; 404. connecting rod; 405. fixing plate; 406. handle; 501. auxiliary groove; 502. second spring; 503. auxiliary plate. DETAILED DESCRIPTION

[0020] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0021] The utility model provides Figure 1-4The environmental DNA rapid sampling device shown in the figure comprises a sampling box 1, one side of the surface of the sampling box 1 is fixedly connected to a support plate 6, the top surface of the support plate 6 is fixedly connected to a water pump 7, the water outlet end of the water pump 7 is fixedly connected to one side of the sampling box 1, the water inlet end of the water pump 7 is fixedly connected to an inlet pipe 8, one end of the inlet pipe 8 is provided with a filter component 3 for filtering impurities in the water body when the water pump 7 extracts samples to prevent the impurities from clogging the water pump 7, the interior of the filter component 3 is provided with a limit component 4 for facilitating the release of the limit of the filter component 3 so as to disassemble and clean it, and the interior of the filter component 3 is provided with a After the limiting component 4 releases the limiting of the filter component 3, the auxiliary component 5 pops out the filter component 3. The bottom of the filter component 3 is provided with a sampling component 2 for sampling the water body through a water pump 7. The water pump 7 provides suction, and then the sampling component 2 is used to sample the water body. The sampled water body will first be filtered through the filtering component 3 to block the debris in the water, so as to prevent the debris in the water from entering the water pump 7 during sampling, causing the water pump 7 to be blocked and affecting the sampling speed of the water pump 7. At the same time, the limiting component 4 and the auxiliary component 5 can be used to quickly disassemble and clean the filter component 3.

[0022] like Figure 1-4 As shown, the filter assembly 3 includes a filter box 301, one side of the surface of the filter box 301 is fixedly connected to one side of the surface of the sampling box 1, sliding grooves 302 are provided on both sides of the inner wall of the filter box 301, and the inner walls of the two sliding grooves 302 are slidably connected with a filter plate 303, and a water inlet 304 is provided at the center of the surface of the filter plate 303 and the filter box 301, and a filter net 305 is fixed to the inner wall of the water inlet 304 on the filter plate 303, and the filter net 305 can filter out debris in the water during sampling, and a baffle 306 is fixedly connected to one end of the filter plate 303.

[0023] like Figure 2-4 As shown, the limiting assembly 4 includes two pairs of limiting grooves 401 opened on both sides of the surface of the filter plate 303, the inner walls of each pair of limiting grooves 401 are slidably connected to the limiting blocks 402, the limiting blocks 402 cooperate with the limiting grooves 401 to limit and fix the filter plate 303, the opposite sides of the two limiting blocks 402 are fixedly connected with a pair of connecting rods 404, the rod walls of each pair of connecting rods 404 are sleeved with a first spring 403, the opposite ends of the two pairs of connecting rods 404 pass through the opposite sides of the two sliding grooves 302 and extend to both sides of the surface of the filter box 301, the limiting assembly 4 also includes two fixing plates 405, the opposite sides of the two fixing plates 405 are respectively fixedly connected to the opposite ends of the two pairs of connecting rods 404, and the opposite sides of the two fixing plates 405 are fixedly connected with handles 406, which facilitate the control of the limiting blocks 402 to release the limit of the filter plate 303.

[0024] like Figure 2As shown, the auxiliary component 5 includes an auxiliary groove 501 opened inside the filter box 301, and two second springs 502 are fixedly connected to one side of the inner wall of the auxiliary groove 501. The second springs 502 can pop out the filter plate 303 by extending themselves after the filter plate 303 is released from the limit. One end of the two second springs 502 is fixedly connected to the auxiliary plate 503, and the side of the auxiliary plate 503 away from the second springs 502 is in contact with one side of the filter plate 303.

[0025] like Figure 1 As shown, the sampling component 2 includes a hose 201, one end of which is fixedly connected to the bottom surface of the filter box 301 and communicates with the water inlet 304, the other end of the hose 201 is fixedly connected to a sampling tube 202, and the end of the sampling tube 202 away from the hose 201 is fixedly connected to a sampling head 203, and sampling is performed through the sampling head 203.

[0026] like Figure 1 and Figure 2 As shown, two fixing clamps 9 are fixedly connected to one side of the surface of the sampling box 1 , and the fixing clamps 9 can fix the sampling tube 202 in the fixing clamps 9 , and the auxiliary groove 501 and the sliding groove 302 are in the same plane.

[0027] Working principle of the utility model: when the environmental DNA rapid sampling device is in use, the device is first transported as a whole to the location where sampling is required, and then the water pump 7 is turned on. After the water pump 7 is turned on, the sampling tube 202 and the sampling head 203 are placed in the water to be sampled, and then the water is drawn into the hose 201 by the suction force of the water pump 7, and then enters the filter box 301, and then the sampled water is filtered by the filter screen 305 on the filter plate 303, and then enters the sampling box 1 through the water inlet pipe 8 and the water pump 7 for storage;

[0028] When the filter screen 305 needs to be cleaned, the two handles 406 are pulled to both sides at the same time, and then the connecting rods 404 are driven to move to both sides through the two fixing plates 405 respectively, and then the two pairs of connecting rods 404 drive the two limit blocks 402 to move to both sides while moving to both sides, so that the two limit blocks 402 and the limit grooves 401 are released from the limit, and then the filter plate 303 is pushed out by the two second springs 502 and the auxiliary plate 503, so that the taken out filter plate 303 and the filter screen 305 can be cleaned;

[0029] After the filter plate 303 and the filter screen 305 are cleaned, the end of the filter plate 303 without the baffle 306 is inserted into the sliding groove 302, and the two handles 406 are pulled to move the two limit blocks 402 to both sides, and the first springs 403 sleeved on the two pairs of connecting rods 404 are in a compressed state. Then, as the filter plate 303 is inserted, the end of the filter plate 303 without the baffle 306 is inserted into the auxiliary groove 501 and contacts the auxiliary plate 503, and then the two second springs 502 are squeezed inward. The pressure makes the two second springs 502 compressed, so that when the filter plate 303 is removed, the filter plate 303 can be pushed out by the force of the two second springs 502 extending, and then when the filter plate 303 is inserted to the maximum limit position, the limit groove 401 and the limit block 402 are aligned, and then the two handles 406 are released at the same time, and then the first springs 403 on the connecting rod 404 are stretched and then drive the two limit blocks 402 to cooperate with the two limit grooves 401 to limit and fix the filter plate 303.

[0030] Finally, it should be noted that the above is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art can still modify the technical solutions described in the aforementioned embodiments or make equivalent substitutions for some of the technical features therein. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. An environmental DNA rapid sampling device, comprising a sampling box (1), characterized in that: A support plate (6) is fixedly connected to one side of the surface of the sampling box (1), a water pump (7) is fixedly connected to the top surface of the support plate (6), a water outlet end of the water pump (7) is fixedly connected to one side of the sampling box (1), a water inlet end of the water pump (7) is fixedly connected to a water inlet pipe (8), one end of the water inlet pipe (8) is provided with a filter assembly (3) for filtering impurities in the water body when the water pump (7) extracts samples, thereby preventing the impurities from clogging the water pump (7), a limit assembly (4) is provided inside the filter assembly (3) for facilitating the release of the limit of the filter assembly (3) for disassembly and cleaning, an auxiliary assembly (5) is provided inside the filter assembly (3) for ejecting the filter assembly (3) after the limit assembly (4) releases the limit of the filter assembly (3), and a sampling assembly (2) is provided at the bottom of the filter assembly (3) for sampling the water body through the water pump (7).

2. The environmental DNA rapid sampling device according to claim 1, characterized in that: The filter assembly (3) comprises a filter box (301), one side of the surface of the filter box (301) is fixedly connected to one side of the surface of the sampling box (1), sliding grooves (302) are provided on both sides of the inner wall of the filter box (301), the inner walls of the two sliding grooves (302) are slidably connected to a filter plate (303), a water inlet (304) is provided at the center of the surface of the filter plate (303) and the filter box (301), a filter net (305) is fixedly connected to the inner wall of the water inlet (304) on the filter plate (303), and a baffle (306) is fixedly connected to one end of the filter plate (303).

3. The environmental DNA rapid sampling device according to claim 2, characterized in that: The limiting assembly (4) comprises two pairs of limiting grooves (401) formed on both sides of the surface of the filter plate (303); the inner walls of each pair of limiting grooves (401) are slidably connected to the limiting blocks (402); opposite sides of the two limiting blocks (402) are fixedly connected to a pair of connecting rods (404); the rod walls of each pair of connecting rods (404) are sleeved with a first spring (403); and opposite ends of the two pairs of connecting rods (404) penetrate the opposite sides of the two sliding grooves (302) and extend to both sides of the surface of the filter box (301).

4. The environmental DNA rapid sampling device according to claim 3, characterized in that: The limiting assembly (4) further comprises two fixing plates (405), opposite sides of the two fixing plates (405) being fixedly connected to opposite ends of the two pairs of connecting rods (404) respectively, and opposite sides of the two fixing plates (405) being fixedly connected to handles (406).

5. The environmental DNA rapid sampling device according to claim 4, characterized in that: The auxiliary component (5) comprises an auxiliary groove (501) opened inside the filter box (301), two second springs (502) are fixedly connected to one side of the inner wall of the auxiliary groove (501), one end of the two second springs (502) are commonly fixedly connected to an auxiliary plate (503), and a side of the auxiliary plate (503) away from the second springs (502) is in contact with a side of the filter plate (303).

6. The environmental DNA rapid sampling device according to claim 1, characterized in that: The sampling assembly (2) comprises a hose (201), one end of the hose (201) being fixedly connected to the bottom surface of the filter box (301) and communicating with the water inlet (304), the other end of the hose (201) being fixedly connected to a sampling tube (202), and the end of the sampling tube (202) away from the hose (201) being fixedly connected to a sampling head (203).

7. The environmental DNA rapid sampling device according to claim 1, characterized in that: Two fixing clips (9) are fixedly connected to one side of the surface of the sampling box (1).

8. The environmental DNA rapid sampling device according to claim 5, characterized in that: The auxiliary groove (501) and the sliding groove (302) are located in the same plane.

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