Enrichment equipment

Abstract

The utility model discloses enrichment equipment, which comprises a gas supply component and a gas outlet component, the at least one water sample accommodating part is used for accommodating a water sample to be enriched, the water sample accommodating part is communicated with the gas supply assembly, and the gas supply assembly is used for providing protective gas for the water sample accommodating part; the speed regulating device is communicated with the water sample accommodating part and is used for controlling the flowing speed of the water sample; the first filtering piece is communicated with the speed regulating device; the second filtering part is communicated with the first filtering part, and the water sample in the water sample accommodating part sequentially passes through the speed regulating device, the first filtering part and the second filtering part and is used for enriching organic matters in the water sample to the second filtering part. According to the utility model, the flowing speed of a water sample can be accurately controlled, and a large-volume water sample can be continuously enriched, so that the quantity of organic matters obtained by enrichment is more, and the quantity of impurities is less.

Description

Technical Field

[0001] The invention relates to the field of environmental monitoring, and in particular to an enrichment device. Background Art

[0002] With the rapid development of economy and society and the continuous improvement of human living standards, the types and quantities of organic matter entering the environmental water bodies are increasing. At present, there are more than 5 million types of organic matter and artificially synthesized organic matter discovered. These organic matter has the characteristics of persistence, bioaccumulation, long-range migration and toxic effects, and can enter various water bodies through different pathways, and even affect some rivers and reservoirs that are sources of drinking water, posing a great threat to drinking water safety.

[0003] The Ames test (also known as the Bacterial Reverse Mutation Test) is a sensitive, rapid, and simple method for detecting DNA base damage caused by genotoxic compounds. It is widely used in the study of the mutagenicity of trace organic matter in water. The test uses nutrient-deficient strains of Salmonella typhimurium or Escherichia coli to be cultured with the tested organic matter. If the tested organic matter has a certain mutagenicity, the defective strain can be reversed and mutated back to the wild type. The wild-type strain can synthesize histidine / tryptophan by itself, can grow and form colonies on a medium without histidine / tryptophan, and the nutrient-deficient strain cannot synthesize histidine / tryptophan by itself, and cannot form colonies on a medium without histidine / tryptophan. Based on this, the mutagenicity of the test substance can be evaluated.

[0004] However, the content of most organic pollutants in water bodies is extremely low and difficult to detect. The commonly used organic matter enrichment methods at home and abroad are resin adsorption separation, reverse osmosis separation and ultrafiltration separation. Environmental conditions such as mud and plankton in the water body and the pore size, material and size of the filter column will affect the enrichment effect to varying degrees. The amount of enriched water samples ranges from a few milliliters to several liters. Manual intervention is required in the enrichment process to adjust components such as burettes, and large volumes of water samples cannot be enriched. The enriched organic matter may contain impurities and is of low quality.

[0005] Currently, there is no systematic and standardized device for enriching organic matter in large-volume water samples, which cannot meet the requirements of the Ames test for monitoring drinking water sources. Summary of the invention

[0006] The purpose of the present invention is to solve the technical problem that a large volume of water samples cannot be enriched with high quality. The present invention provides an enrichment device that can enrich a large volume of water samples to obtain enriched samples that can meet the requirements in both quality and quantity.

[0007] In order to solve the above technical problems, an embodiment of the present invention discloses an enrichment device, comprising:

[0008] Air supply components;

[0009] At least one water sample receiving portion, used to receive the water sample to be enriched, the water sample receiving portion being in communication with the gas supply component, the gas supply component being used to provide protective gas to the water sample receiving portion;

[0010] A speed regulating device, the speed regulating device is communicated with the water sample containing portion and is used to control the flow speed of the water sample;

[0011] a first filter element, the first filter element being in communication with the speed regulating device;

[0012] The second filter element is connected to the first filter element, and the water sample in the water sample receiving part passes through the speed regulating device, the first filter element, and the second filter element in sequence, so as to enrich the organic matter in the water sample to the second filter element.

[0013] By adopting the above technical solution, protective gas can be provided to the water sample holding part through the air supply component, so that the water sample flows into the speed regulating device. Through the cooperation of the air supply component and the speed regulating device, the flow speed of the water sample can be accurately controlled, and then the residence time of the water sample in the second filter element is adjusted, so that the organic matter in the water sample can be fully enriched in the second filter element. The above process does not require manual operation and can continuously enrich large volumes of water samples, so that the amount of enriched organic matter is larger and the impurities are less.

[0014] According to another specific embodiment of the present invention, an embodiment of the present invention discloses an enrichment device, wherein the water sample containing portion includes a first sealing portion, the first sealing portion includes an inlet and an outlet, the inlet is used for the protective gas or the water sample to enter, and the outlet is used for the water sample to flow out.

[0015] The adoption of the above technical solution can improve the sealing performance of the water sample containing portion. On the one hand, it can prevent the water sample from escaping from the water sample containing portion, and on the other hand, it can prevent the water sample from contacting with the air.

[0016] According to another specific embodiment of the present invention, an embodiment of the present invention discloses an enrichment device, wherein the water sample holding portion includes a first seal and a second seal, wherein the first seal is disposed at the outlet, and the second seal is disposed at the inlet, and along a first direction, the height of the first seal is higher than that of the second seal.

[0017] By adopting the above technical solution, the sealing performance of the water sample holding part can be further improved by setting the first seal and the second seal. In addition, the height of the first seal is higher than the second seal, which is convenient for distinguishing the inlet and the outlet and avoiding enrichment failure caused by misoperation.

[0018] According to another specific embodiment of the present invention, an embodiment of the present invention discloses an enrichment device, wherein the number of the water sample holding part is one, the inlet is connected to the air supply component, the outlet is connected to the speed regulation device, and the air supply component provides the protective gas to the water sample holding part through the inlet, so as to make the water sample flow into the speed regulation device through the outlet.

[0019] By adopting the above technical solution, water samples are stored in one water sample container, which can reduce costs.

[0020] According to another specific embodiment of the present invention, an embodiment of the present invention discloses an enrichment device, wherein the number of the water sample containing parts is multiple, the multiple water sample containing parts are connected in series in sequence, and the air supply component is connected to any one of the water sample containing parts.

[0021] By adopting the above technical solution, multiple water sample containing parts are used to store the same amount of water, so that the volume of each water sample containing part can be smaller, which is convenient for sampling and transportation.

[0022] According to another specific embodiment of the present invention, an embodiment of the present invention discloses an enrichment device, wherein the water sample receiving part includes 60 liters of the water sample, the number of the multiple water sample receiving parts is 6, and each of the water sample receiving parts includes 10 liters of the water sample.

[0023] By adopting the above technical scheme, 60L of water sample is enriched to obtain the amount of organic matter that meets the requirements of the Ames test.

[0024] According to another specific embodiment of the present invention, an embodiment of the present invention discloses an enrichment device, wherein the speed regulating device comprises an elastic tube and an extrusion portion, one end of the elastic tube is connected to the water sample receiving portion, and the other end is connected to the first filter element, and the extrusion portion can be switched between a first position and a second position;

[0025] In the first position, the squeezing portion squeezes the elastic tube, the elastic tube is closed, and the water sample stops flowing into the first filter element; in the second position, the squeezing portion does not contact the elastic tube, the elastic tube is open, and the water sample flows into the first filter element.

[0026] By adopting the above technical solution, by controlling the frequency of switching the extrusion part between the first position and the second position, it is possible to control the amount of water sample flowing into the first filter element, thereby controlling the flow rate of the water sample, so that the water sample can stay in the second filter element for a longer time, so as to fully enrich the organic matter in the water sample into the second filter element.

[0027] According to another specific embodiment of the present invention, an embodiment of the present invention discloses an enrichment device, wherein the first filter element includes at least one first filter portion and at least one quartz sand filter portion, the first filter portion is arranged at one end of the first filter element connected to the speed regulation device, and is used for performing initial filtration on the water sample, and the quartz sand filter portion is stacked on the first filter portion, and is used for supporting the first filter portion and re-filtering the water sample that has been initially filtered.

[0028] With the above technical solution, on the one hand, since the first filter part (such as quartz wool) is easily squeezed and deformed under nitrogen pressure, affecting the filtering efficiency, the quartz sand filter part is stacked on the first filter part. Since the shape of the quartz sand is fixed, it can provide support for the first filter part and avoid affecting the filtering efficiency. On the other hand, the solution of stacking the quartz sand filter part and the first filter part can ensure high filtering efficiency and prevent the first filter from being blocked.

[0029] According to another specific embodiment of the present invention, an embodiment of the present invention discloses an enrichment device, wherein the second filter element comprises a second filter portion and a third filter portion, the second filter element comprises a water inlet end and a water outlet end, the water inlet end is connected to the first filter element, and the water outlet end is used to discharge waste liquid;

[0030] The second filter part is arranged at the water inlet end, and is used to filter the water sample and prevent the water inlet end from being blocked. The third filter part is arranged at the water outlet end, and is used to prevent the waste liquid from being sucked back into the second filter element.

[0031] By adopting the above technical scheme, the filtering effect can be further improved by setting up a second filter part and a third filter part, and the second filter part is arranged at the water inlet end, which can also prevent the water inlet end from being blocked and affecting the enrichment efficiency; the third filter part is arranged at the water outlet end, which can prevent the waste liquid from being sucked back into the second filter element, and can also prevent the large-pore resin from flowing out of the second filter element and causing enrichment failure.

[0032] According to another specific embodiment of the present invention, an embodiment of the present invention discloses an enrichment device, wherein the second filter element comprises a large-pore resin, and the large-pore resin is disposed between the second filter part and the third filter part for enriching organic matter in the water sample.

[0033] According to another specific embodiment of the present invention, an embodiment of the present invention discloses an enrichment device, wherein the first filter part, the second filter part and the third filter part are all filter cottons.

[0034] By adopting the above technical solution and using filter cotton as the filter medium, a higher filtering efficiency can be obtained.

[0035] According to another specific embodiment of the present invention, an embodiment of the present invention discloses an enrichment device, the second filter element includes a second sealing portion, the enrichment device includes an elastic fastener, the second sealing portion is arranged at the water inlet end, the second sealing portion includes a fluid through hole for supplying water samples or leaching liquid to enter the second filter element, and the elastic fastener is simultaneously engaged with the second sealing portion and the second filter element.

[0036] According to another specific embodiment of the present invention, an embodiment of the present invention discloses an enrichment device, the elastic fastener includes a first part, a second part and a third part, along the first direction, the first part and the second part are spaced apart at both ends of the third part, the second filter element is clamped to the first part, and the second sealing part is clamped to the second part.

[0037] By adopting the above technical solution, the second sealing part is clamped to the second filter element through an elastic fastener, which can not only ensure that the second filter element has a good sealing effect, but also facilitate opening the second sealing part and filling the second sealing element with filtering and adsorption materials such as large-pore resin.

[0038] According to another specific embodiment of the present invention, an embodiment of the present invention discloses an enrichment device, wherein the ratio of the diameter to the length of the second filter element is 1:4 to 1:10, and the length of the second filter element is not less than 10 cm.

[0039] The above technical solution can make the water sample fully contact with the large-pore resin in the second filter element, and at the same time avoid the problem of clogging caused by the small diameter of the second filter element during the later stage of enrichment and filtration.

[0040] According to another specific embodiment of the present invention, an embodiment of the present invention discloses an enrichment device, wherein the first filter element is connected to the second filter element through a hose.

[0041] By adopting the above technical solution, compared with the fixed connection between the first filter element and the second filter element, the arrangement of the first filter element and the second filter element in this solution is more flexible. BRIEF DESCRIPTION OF THE DRAWINGS

[0042] Figure 1 Schematic diagram showing the enrichment device in some embodiments.

[0043] Figure 2 An overall schematic diagram of the enrichment device provided in an embodiment of the present application is shown.

[0044] Figure 3 A top view of the cover of the water sample receiving portion of the enrichment device provided in an embodiment of the present application is shown.

[0045] Figure 4 A partially enlarged schematic diagram of the water sample receiving portion of the enrichment device provided in an embodiment of the present application is shown.

[0046] Figure 5 A front view of the cover of the water sample receiving portion of the enrichment device provided in an embodiment of the present application is shown.

[0047] Figure 6 A partial schematic diagram of the water sample containing portion of the enrichment device provided in an embodiment of the present application is shown.

[0048] Figure 7 A schematic diagram of a speed regulating device of an enrichment device provided in an embodiment of the present application is shown.

[0049] Figure 8 A partial cross-sectional view of the second filter element of the enrichment device provided in an embodiment of the present application is shown.

[0050] Fig. 9 A schematic diagram of the second sealing portion of the second filter element of the enrichment device provided in an embodiment of the present application is shown.

[0051] Fig.10 A top view of the elastic fastener of the enrichment device provided in an embodiment of the present application is shown.

[0052] Fig.11 A front view of the elastic fastener of the enrichment device provided in the embodiment of the present application is shown. DETAILED DESCRIPTION

[0053] The following specific embodiments illustrate the implementation of the present invention, and those skilled in the art can easily understand other advantages and effects of the present invention from the contents disclosed in this specification. Although the description of the present invention will be introduced in conjunction with the preferred embodiment, this does not mean that the features of this invention are limited to this implementation. On the contrary, the purpose of introducing the invention in conjunction with the implementation is to cover other options or modifications that may extend based on the claims of the present invention. In order to provide a deep understanding of the present invention, the following description will include many specific details. The present invention can also be implemented without using these details. In addition, in order to avoid confusion or blurring the focus of the present invention, some specific details will be omitted in the description. It should be noted that the embodiments of the present invention and the features in the embodiments can be combined with each other without conflict.

[0054] It should be noted that in this specification, similar reference numerals and letters denote similar items in the following drawings, and therefore, once an item is defined in one drawing, it does not need to be further defined and explained in the subsequent drawings.

[0055] In the description of this embodiment, it should be noted that the terms "upper", "lower", "inner", "bottom", etc. indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, or are the orientations or positional relationships in which the inventive product is usually placed when used. They are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present invention.

[0056] The terms “first”, “second”, etc. are only used for distinguishing descriptions and should not be understood as indicating or implying relative importance.

[0057] In the description of this embodiment, it is also necessary to explain that, unless otherwise clearly specified and limited, the terms "set", "connected", and "connected" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in this embodiment can be understood according to specific circumstances.

[0058] In order to make the objectives, technical solutions and advantages of the present invention more clear, the embodiments of the present invention will be further described in detail below with reference to the accompanying drawings.

[0059] In some embodiments, see Figure 1 The enrichment device includes a burette 100, a support frame 200 and a water sample bucket (not shown in the figure). The burette 100 is fixed on the support frame 200. The burette 100 includes a liquid inlet end 101 and a liquid outlet end 102. The liquid inlet end 101 is connected to the water sample bucket, and the liquid outlet end 102 is used to discharge waste liquid. The liquid outlet end 102 also includes a knob 1021 and a flow stop 1022. The knob 1021 is connected to the flow stop 1022. The flow stop 1022 is arranged through the burette 100. By rotating the knob 1021, the position of the flow stop 1022 in the burette 100 can be controlled to adjust the speed at which the water sample flows out of the burette 100.

[0060] When enriching water samples, it is necessary to continuously rotate the knob 1021 to obtain a relatively stable water sample outflow rate. When enriching a large volume of water samples, since the enrichment time of large volume water samples is relatively long, for example, when enriching a 60L water sample, in order to meet the requirements of the Ames test, an enrichment time of at least 6 days is usually required. When using the burette 100 for enrichment, after the operator adjusts the position of the flow stop 1022, as the enrichment time increases, the water sample outflow rate at the liquid outlet 102 will gradually slow down or even stop flowing out. Therefore, it is necessary to manually intervene from time to time to fine-tune the burette 100 so that the water sample can maintain a relatively stable outflow rate.

[0061] It can be seen that the above scheme for enriching large-volume water samples has at least the following disadvantages: ① The flow rate of the water sample is unstable, which affects the enrichment effect. ② The enrichment process requires manual intervention to operate the instrument from time to time. For the enrichment of large-volume water samples, it takes a long time. Therefore, it is difficult to operate the instrument by manual intervention from time to time in practice. Therefore, it is understandable that some of the equipment used for enriching water samples in the field in the prior art is also difficult to use for long-term continuous enrichment of large-volume water samples, and the enriched samples obtained by the equipment for enriching water samples in the field need to be transported back to the laboratory, and the preservation of the enriched samples is difficult.

[0062] Based on this, the present application provides, in some embodiments, an enrichment device, see Figure 2 , including an air supply component 10, a speed regulating device 20, a first filter 30, a second filter 40 and at least one water sample receiving part 50. The water sample receiving part 50 is used to accommodate the water sample to be enriched, the water sample receiving part 50 is connected to the air supply component 10, and the air supply component 10 is used to provide protective gas to the water sample receiving part 50; the speed regulating device 20 is connected to the water sample receiving part 50, and is used to control the flow speed of the water sample; the first filter 30 is connected to the speed regulating device 20; the second filter 40 is connected to the first filter 30, and the water sample in the water sample receiving part 50 passes through the speed regulating device 20, the first filter 30, and the second filter 40 in sequence, and is used to enrich the organic matter in the water sample to the second filter 40. Exemplarily, the air supply component 10, the speed regulating device 20, the first filter 30, the second filter 40 and the at least one water sample receiving part 50 are all connected by a hose 60, so that it is convenient to arrange the aforementioned components or devices in the laboratory. It is understandable that the aforementioned components may also be connected through other means such as PVC pipes, and this application does not limit this.

[0063] By adopting the above technical solution, protective gas can be provided to the water sample containing part 50 through the air supply component 10, so that the water sample flows into the speed regulating device 20. Through the cooperation between the air supply component 10 and the speed regulating device 20, the flow speed of the water sample can be accurately controlled, and then the residence time of the water sample in the second filter element 40 is adjusted, so that the organic matter in the water sample can be fully enriched in the second filter element 40. The above process does not require manual operation and can continuously enrich large volumes of water samples to obtain enriched samples with guaranteed quality and quantity.

[0064] In some embodiments, the protective gas provided by the gas supply assembly 10 is nitrogen, and the gas supply assembly 10 includes a gas supply unit 101 and a control unit (not shown in the figure), the gas supply unit 101 and the control unit are connected, and the control unit can adjust the pressure of the nitrogen gas charged into the water sample receiving unit 50 by the gas supply unit 101 to control the rate at which the water sample flows into the water sample receiving unit 50. Exemplarily, the control unit controls the output pressure of the nitrogen gas to be 0.1Mpa.

[0065] On the one hand, nitrogen is a highly stable protective gas that can protect organic compounds in the water that are sensitive to oxygen or moisture and prevent them from undergoing unnecessary oxidation or hydrolysis reactions; on the other hand, when the water sample comes into contact with nitrogen, the flow of nitrogen can drive the volatile organic compounds escaping from the surface of the solution into the second filter element 40, thereby enhancing the filtering and enrichment effect.

[0066] It is understandable that the protective gas that the gas supply assembly 10 can provide can also be other gases with stable chemical properties such as argon, and the present application does not limit this.

[0067] In some embodiments, see Figure 3 , Figure 4 , Figure 5 Combined with Figure 2 The water sample receiving portion 50 includes a first sealing portion 501, the first sealing portion 501 includes an inlet 5011 and an outlet 5012, the inlet 5011 is used for the protective gas or water sample to enter, and the outlet 5012 is used for the water sample to flow out. Exemplarily, the water sample receiving portion 50 includes a box body 502, the first sealing portion 501 includes a cover body 5013 and a first convex portion 5014, the first convex portion 5014 is convexly arranged on the box body 502 along the first direction X, the first convex portion 5014 includes a first sealing ring 5015, the first sealing ring 5015 is arranged on the side of the first convex portion 5014 facing away from the box body 502 in the first direction X, and the first convex portion 5014 and the cover body 5013 are connected by threads. The inlet 5011 and the outlet 5012 are spaced apart from each other on the cover body 5013 along the second direction Y.

[0068] In some embodiments, the water sample receiving portion 50 includes a first seal 503 and a second seal 504, wherein the first seal 503 is disposed at the outlet 5012, and the second seal 504 is disposed at the inlet 5011. Along the first direction X, the height of the first seal 503 is higher than that of the second seal 504. Exemplarily, the first seal 503 includes a first threaded connection column 5031, wherein one end of the first threaded connection column 5031 is connected to the hose 60 along the first direction X, and the other end is connected to the inlet 5011 by threading, so as to seal and fix the hose 60 to the first seal 503. The second seal 504 includes a second threaded connection column 5041, wherein one end of the second threaded connection column 5041 is connected to the hose 60 along the first direction X, and the other end is connected to the outlet 5012 by threading, so as to seal and fix the hose 60 to the second seal 504. The height of the first threaded connection column 5031 is higher than that of the second threaded connection column 5041, so as to facilitate the distinction between the inlet 5011 and the outlet 5012.

[0069] Exemplarily, the first seal 503 includes a second seal ring 5032 and a nut 5033, and the second seal ring 5032 is disposed between the nut 5033 and the first threaded connection column 5031. The second seal 504 includes a third seal ring (not shown in the figure), and the third seal ring is disposed between the outlet 5012 and the second threaded connection column 5041, and is used to enhance the sealing performance of the first seal 503 and the second seal 504. It can be understood that the specific forms of the first seal 503 and the second seal 504 are not fixed. For example, the second seal 504 may also include a nut 5033, and the nut 5033 is disposed between the outlet 5012 and the second threaded connection column 5041.

[0070] Exemplarily, the water sample containing portion 50 is in the shape of a rectangular parallelepiped. It can be understood that the water sample containing portion 50 can also be in other shapes such as a cylindrical shape, and the present application does not impose any limitation on this.

[0071] In some embodiments, see 3. Figure 4 , Figure 6 Combined with Figure 2 The water sample receiving portion 50 includes an internal drain pipe 505, which is extended along the first direction X and communicated with the outlet 5012, so as to discharge the water sample from the water sample receiving portion 50. In some embodiments, the first sealing portion 501 includes a metal pendant 5016, which is disposed on a side of the cover 5013 facing away from the first sealing member 503 along the first direction X, and is disposed at an end of the internal drain pipe 505 away from the cover 5013 along the first direction X, so as to ensure that the internal drain pipe 505 is always located at the bottom of the water sample receiving portion 50, so that the water sample can be discharged from the outlet 5012 under the pressure of the protective gas.

[0072] In some embodiments, in order to meet the requirements of the Ames test, combined with the daily drinking water required for normal people to maintain life of 2 to 3L, the water sample receiving part 50 provided in the present application includes 60 liters of water sample. In some embodiments, the number of water sample receiving parts 50 is one, the inlet 5011 is connected to the air supply component 10, and the outlet 5012 is connected to the speed regulating device 20. The air supply component 10 provides protective gas to the water sample receiving part 50 through the inlet 5011, so that the water sample flows into the speed regulating device 20 through the outlet 5012. In some embodiments, the number of water sample receiving parts 50 is multiple, and the multiple water sample receiving parts 50 are connected in series in sequence, and the air supply component 10 is connected to any one of the water sample receiving parts 50. Exemplarily, the number of water sample containing portions 50 is 6, each water sample containing portion 50 includes 10 liters of water sample, the inlet 5011 of any water sample containing portion 50 is connected to the air supply assembly 10, and the inlet 5011 of the remaining water sample containing portions 50 are connected to the outlet 5012 of the previous water sample containing portion 50 in sequence, so as to supply water samples into the water sample containing portions 50 connected in series.

[0073] It can be understood that the water sample holding portion 50 can include water samples of small volumes such as 1 liter, 2 liters, 3 liters, and 4 liters, and can also include water samples of large volumes such as 50 liters, 70 liters, and 80 liters. The number of water sample holding portions 50 can also be 2, 3, 4, 5, 7, 8, etc., and the present application does not impose any limitation on this.

[0074] In some embodiments, see Figure 7 The speed regulating device 20 includes an elastic tube 201 and a pressing part 202. One end of the elastic tube 201 is connected to the water sample receiving part 50, and the other end is connected to the first filter element 30. The pressing part 202 can be switched between a first position and a second position. In the first position, the pressing part 202 presses the elastic tube 201, the elastic tube 201 is closed, and the water sample stops flowing into the first filter element 30. In the second position, the pressing part 202 does not contact the elastic tube 201, the elastic tube 201 is connected, and the water sample flows into the first filter element 30. Exemplarily, the speed regulating device 20 includes a housing 203, the housing 203 includes a receiving groove 2031, the pressing part 202 is disposed in the receiving groove 2031, and the elastic tube 201 is disposed between the pressing part 202 and the wall of the receiving groove. Exemplarily, the extrusion portion 202 includes a driving rod 2021 and a roller 2022. The driving rod 2021 can rotate along the circumferential direction R under the action of a motor (not shown in the figure). The roller 2022 is arranged at one end of the driving rod 2021, and the elastic tube 201 is arranged between the roller 2022 and the wall of the accommodating groove.

[0075] When the driving rod 2021 rotates to the first position a, the roller 2022 squeezes the elastic tube 201, and the elastic tube 201 is pressed to form a closed cut-off point, thereby forming a negative pressure in the elastic tube 201 and sucking in liquid. When the driving rod 2021 continues to rotate to the second position b, the roller 2022 leaves the elastic tube 201, and the elastic tube 201 rebounds to the conducting state, allowing the water sample to flow out of the speed regulating device 20. The flow direction of the water sample is shown by the arrow at c in the figure.

[0076] By changing the frequency of the extrusion part 202 switching between the first position a and the second position b, the elastic tube 201 can be continuously switched between the on and off states, thereby adjusting the speed at which the water sample flows out of the speed regulating device 20. Exemplarily, the speed regulating device is a peristaltic pump, and by adjusting the air supply pressure of the air supply assembly in cooperation with the peristaltic pump, the flow rate of the water sample is about 30 to 40 ml per minute.

[0077] In some embodiments, see Figure 2 , the first filter element 30 includes at least one first filter part 301 and at least one quartz sand filter part 302, the first filter part 301 is arranged at one end of the first filter element 30 connected to the speed regulating device 20, and is used to perform preliminary filtering on the water sample, and the quartz sand filter part 302 is stacked on the first filter part 301, and is used to support the first filter part 301 and filter the water sample after preliminary filtering again. Exemplarily, the first filter element 30 is cylindrically arranged, and the first filter element 30 includes two first filter parts 301 and two quartz sand filter parts 302, wherein, along the first direction X (i.e., the height direction of the first filter element 30), each first filter part 301 and each quartz sand filter part 302 are arranged adjacently, and the quartz sand filter part 302 can remove impurities and sediment in the water sample on the one hand, and on the other hand, the quartz sand has a fixed shape, and can also support the first filter part 301, so as to prevent the first filter part 301 from being squeezed and deformed under the nitrogen pressure, thereby affecting the filtering efficiency. Exemplarily, the first filter element 30 includes a quartz sand column with a mesh size of 8 to 16.

[0078] In some embodiments, see Figure 8 Combined with Figure 2 The second filter element 40 includes a second filter part 401 and a third filter part 402. The second filter element 40 includes a water inlet end 403 and a water outlet end 404. The water inlet end 403 is connected to the first filter element 30, and the water outlet end 404 is used to discharge waste liquid. The second filter part 401 is arranged at the water inlet end 403, and is used to filter the water sample and prevent the water inlet end 403 from being blocked. The third filter part 402 is arranged at the water outlet end 404, and is used to prevent the waste liquid from being sucked back into the second filter element 40, resulting in enrichment failure. Exemplarily, the second filter element 40 is cylindrical. It can be understood that the first filter element 30 and the second filter element 40 can also be other shapes such as a rectangular parallelepiped, and this application does not limit this.

[0079] In some embodiments, see Figure 8 Combined with Figure 2 , the second filter element 40 includes a macroporous resin 405, which is arranged between the second filter part 401 and the third filter part 402, and is used to enrich organic matter in the water sample. The macroporous resin 405 refers to a resin with a pore size between 10 and 500 μm, which has a stronger adsorption and penetration ability for macromolecules. In addition, the macroporous resin 405 has a large surface area, which can improve the adsorption efficiency and resolution, and because the structure of the macroporous resin 405 is stable, its service life is longer than that of ordinary resins. Exemplarily, there is 30 ml of activated macroporous resin 405 in the second filter element 40, and the activation of the macroporous resin 405 refers to rinsing the macroporous resin 405 with pure water and methanol, and soaking it in organic solvents (methanol, dichloromethane, cyclohexane, acetonitrile) in a Soxhlet extractor for 8 to 24 hours to remove organic matter and complete the activation. The activated resin is soaked in methanol for standby use, and the second filter element is cleaned with distilled water 3 to 4 times before use.

[0080] Exemplarily, the first filter part 301, the second filter part 401 and the third filter part 402 are all filter cotton. It can be understood that the filter cotton can include quartz filter cotton, glass fiber filter cotton and other types, and the present application does not limit the specific material of the filter cotton.

[0081] In some embodiments, see Figure 8 , Fig. 9 Combined with Figure 2 The second filter element 40 includes a second sealing portion 406, which is disposed at the water inlet end 403. The second sealing portion 406 includes a fluid through hole 4061 for supplying a water sample or a washing liquid into the second filter element 40. Exemplarily, the second sealing portion 406 includes a first connecting portion 4062 and a second convex portion 4063, the first connecting portion 4062 is extended along the second direction Y, the fluid through hole 4061 is disposed at the first connecting portion 4062 and the second convex portion 4063, the second convex portion 4063 is convexly disposed at the first connecting portion 4062 along the first direction X, the second convex portion 4063 is disposed inside the second filter element 40, a fourth sealing ring (not shown in the figure) is disposed at one end of the first connecting portion 4062 away from the second convex portion 4063, and the first connecting portion 4062 abuts against the water inlet end 403.

[0082] In some embodiments, see Figure 8 , Fig.10 , Fig.11 Combined with Figure 2The enrichment device includes an elastic fastener 70, which is clamped with the second sealing portion 406 and the second filter element 40 at the same time, and is used to connect the second sealing portion 406 with the water inlet end 403. In some embodiments, the elastic fastener 70 includes a first part 701, a second part 702 and a third part 703, along the first direction X, the first part 701 and the second part 702 are spaced apart at both ends of the third part 703, the second filter element 40 is clamped with the first part 701, and the second sealing portion 406 is clamped with the second part 702. Exemplarily, the first part 701, the second part 702, and the third part 703 are all arranged in an arc shape. The arc-shaped side wall 7011 of the first part 701 can be arranged to fit with the side of the second filter element 40. Along the first direction X, the third part 703 includes an arc-shaped upper surface 7031 and an arc-shaped lower surface 7032 that are relatively arranged, and the second protrusion 4063 abuts against the arc-shaped lower surface 7032 of the third part 703. It can be understood that the first part 701, the second part 702, and the third part 703 can also be other shapes such as polygonal shapes, and the present application does not limit this.

[0083] For example, see Fig.10 , Fig.11 , along the second direction Y, the second part 702 is arranged on one side of the first part 701 and the third part 703, the side of the first part 701 facing away from the second part 702 includes a plurality of recessed portions 704 and a plurality of protrusions 705 arranged at intervals along the circumferential direction R, and each recessed portion 704 and each protrusion 705 are arranged adjacent to each other, and the side of the third part 703 facing away from the second part 702 also includes a plurality of recessed portions 704 and a plurality of protrusions 705 arranged at intervals along the circumferential direction R, and each recessed portion 704 and each protrusion 705 are arranged adjacent to each other.

[0084] In some embodiments, the ratio of the diameter to the length of the second filter element 40 is 1:4 to 1:10, and the length of the second filter element 40 is not less than 10 cm, so that the water sample is fully in contact with the large-pore resin 405 in the second filter element 40 to obtain a better enrichment effect. For example, the ratio of the diameter to the length of the second filter element 40 can be 1:5, 1:6, 1:7, 1:8, 1:9, and this application does not limit this.

[0085] In some embodiments, after enrichment, the organic matter adsorbed on the large-pore resin is washed with 100 ml of 30% acetone-methanol solution for 10 minutes, and then slowly dripped, the eluate is collected in a sterile conical flask, and the eluate is transferred to an evaporating dish and evaporated to dryness in a 45°C water bath, and dissolved with an appropriate amount of dimethyl sulfoxide (DMSO) for later use.

[0086] In order to fully illustrate that the enrichment device provided in the embodiment of the present application has a better enrichment effect, the present application will be explained in conjunction with example data of the Ames test.

[0087] Table 1 Results of water sample enrichment with TA98 strain Ames

[0088]

[0089] As shown in Table 1, in some embodiments, a blank group, a control group and a test group are set up, wherein the blank group refers to culturing the TA98 strain in the culture medium with the solvent dimethyl sulfoxide as the sample, the control group is to enrich the provided water sample with the relevant equipment of the prior art, and then use it to cultivate the TA98 strain in the culture medium, and the test group is to enrich the provided water sample with the enrichment equipment provided in the present application, and then use it to cultivate the TA98 strain in the culture medium.

[0090] The TA98 strain can be used to detect frameshift mutagens. It should be noted that mutagens are also called mutagens, which can cause the histidine-deficient mutant of Salmonella typhimurium to revert to the wild type. Therefore, the number of colonies generated on the culture medium can be used to determine whether the test substance is a mutagen. For indirect mutagens, the S9 mixed solution + TA98 strain can be used for testing. It is understandable that the present application does not limit the specific strain type. For example, other strains such as TA100 strain, TA102 strain, TA97 strain, TA1535 strain, etc. can also be selected for culture detection.

[0091] Exemplarily, SD in Table 1 represents the standard deviation of the data obtained from three tests, and the minimum mutation amount is the sample dose that causes 2 times the number of blank group reversal colonies when the test substance presents a positive reaction (the number of reversal colonies per dish is equal to or greater than 2 times the number of blank group reversal colonies and there is a dose-response relationship). The blank group refers to the TA98 strain when the dimethyl sulfoxide inoculation volume is 0.1 ml, and three parallel culture tests are carried out on the TA98 strain. The number of strains on the culture medium obtained is: 26, 32, and 27, respectively, and the average of the three tests is 28.3 strains. It can be understood that three parallel culture tests are also carried out on the TA98 strain + S9 mixed solution, and the number of strains on the culture medium obtained is: 41, 40, and 44, respectively, and the average of the three tests is 41.7 strains.

[0092] When the number of TA98 strains in the control group or the test group is greater than or equal to 56.6, and the gradients of different sample water samples show a dose-response relationship, the water sample is detected positive, and the minimum mutation amount under the condition of TA98 strain is calculated according to the dose-response relationship. When the number of strains under the condition of TA98 strain + S9 mixed solution in the control group or the test group is greater than or equal to 83.4, and the gradients of different sample water samples show a dose-response relationship, the water sample is detected positive, and the minimum mutation amount under the condition of TA98 strain + S9 mixed solution is calculated according to the dose-response relationship.

[0093] The control group and the test group of the embodiment of the present application are tested with gradient water sample volumes of 0.25L, 0.5L, 1L, and 2L. According to the obtained data, a linear relationship between the number of colonies and the amount of water sample can be obtained. From the relevant linear relationship, it can be known that: the minimum mutation amount in the control group is 1.417L, that is, when the water sample volume is 1.417L, the number of strains in the control group is 56.6; the minimum mutation amount in the test group is 0.738L, that is, when the water sample volume is 0.738L, the number of strains in the control group is 56.6. It can be seen that the enrichment device provided by the present application has a better enrichment effect under the same conditions compared with the related equipment of the prior art, that is, the number of organic matter that can be enriched is greater, so the minimum mutation amount is lower.

[0094] In addition, under the condition of TA98 strain + S9 mixed solution, no positive was detected in the water sample enriched by the prior art, but after the same water sample was enriched by the enrichment device provided by the present application, positive was detected and the minimum mutation amount was 1.504 L. It can be seen that the enrichment effect of the enrichment device provided by the present application is better, and the enriched water sample can obtain more accurate results when used for the Ames test.

[0095] Although the present invention has been illustrated and described with reference to certain preferred embodiments of the present invention, it should be understood by those skilled in the art that the above is a further detailed description of the present invention in conjunction with specific embodiments, and it cannot be determined that the specific implementation of the present invention is limited to these descriptions. Those skilled in the art may make various changes in form and details, including making several simple deductions or substitutions, without departing from the spirit and scope of the present invention.

Claims

1. An enrichment device, characterized in that: include: Air supply components; At least one water sample receiving portion, used to receive the water sample to be enriched, the water sample receiving portion being in communication with the gas supply component, the gas supply component being used to provide protective gas to the water sample receiving portion; A speed regulating device, the speed regulating device is communicated with the water sample containing portion and is used to control the flow speed of the water sample; a first filter element, the first filter element being in communication with the speed regulating device; The second filter element is connected to the first filter element, and the water sample in the water sample receiving part passes through the speed regulating device, the first filter element, and the second filter element in sequence, so as to enrich the organic matter in the water sample to the second filter element.

2. The enrichment device according to claim 1, characterized in that The water sample containing portion comprises a first sealing portion, and the first sealing portion comprises an inlet and an outlet, wherein the inlet is used for allowing the protective gas or the water sample to enter, and the outlet is used for allowing the water sample to flow out.

3. The enrichment device according to claim 2, characterized in that The water sample containing part includes a first sealing member and a second sealing member. The first sealing member is arranged at the outlet, and the second sealing member is arranged at the inlet. Along the first direction, the height of the first sealing member is higher than that of the second sealing member.

4. The enrichment device according to claim 3, characterized in that The number of the water sample containing part is one, the inlet is connected to the air supply component, the outlet is connected to the speed regulating device, and the air supply component provides the protective gas to the water sample containing part through the inlet, so that the water sample flows into the speed regulating device through the outlet.

5. The enrichment device according to claim 3, characterized in that There are multiple water sample containing parts, and the multiple water sample containing parts are connected in series in sequence. The air supply component is connected to any one of the water sample containing parts.

6. The enrichment device according to claim 5, characterized in that The water sample containing part includes 60 liters of the water sample, the number of the multiple water sample containing parts is 6, and each of the water sample containing parts includes 10 liters of the water sample.

7. The enrichment device according to any one of claims 1 to 6, characterized in that: The speed regulating device comprises an elastic tube and an extrusion part, one end of the elastic tube is connected to the water sample receiving part, and the other end is connected to the first filter element, and the extrusion part can be switched between a first position and a second position; In the first position, the squeezing portion squeezes the elastic tube, the elastic tube is closed, and the water sample stops flowing into the first filter element; In the second position, the pressing portion does not contact the elastic tube, the elastic tube is open, and the water sample flows into the first filter element.

8. The enrichment device according to any one of claims 1 to 6, characterized in that: The first filter element includes at least one first filter portion and at least one quartz sand filter portion. The first filter portion is arranged at one end of the first filter element connected to the speed regulating device, and is used to perform initial filtration on the water sample. The quartz sand filter portion is stacked on the first filter portion, and is used to support the first filter portion and re-filter the water sample that has been initially filtered.

9. The enrichment device according to claim 8, characterized in that The second filter element comprises a second filter portion and a third filter portion, the second filter element comprises a water inlet end and a water outlet end, the water inlet end is communicated with the first filter element, and the water outlet end is used to discharge waste liquid; The second filter part is arranged at the water inlet end, and is used to filter the water sample and prevent the water inlet end from being blocked. The third filter part is arranged at the water outlet end, and is used to prevent the waste liquid from being sucked back into the second filter element.

10. The enrichment device according to claim 9, characterized in that The second filter element comprises a large-pore resin, which is arranged between the second filter part and the third filter part and is used for enriching organic matter in the water sample.

11. The enrichment device according to claim 9, characterized in that The first filter part, the second filter part and the third filter part are all filter cotton.

12. The enrichment device according to claim 9, characterized in that The second filter element includes a second sealing portion, the enrichment device includes an elastic fastener, the second sealing portion is arranged at the water inlet end, the second sealing portion includes a fluid through hole for supplying water samples or leaching liquid to enter the second filter element, and the elastic fastener is simultaneously engaged with the second sealing portion and the second filter element.

13. The enrichment device according to claim 12, characterized in that The elastic fastener includes a first part, a second part and a third part. Along the first direction, the first part and the second part are spaced apart at two ends of the third part. The second filter element is clamped to the first part, and the second sealing part is clamped to the second part.

14. The enrichment device according to claim 1, characterized in that The second filter element is a cylinder, the ratio of the diameter to the length of the second filter element is 1:4 to 1:10, and the length of the second filter element is not less than 10 cm.

15. The enrichment device according to claim 1, characterized in that The first filter element is communicated with the second filter element through a hose.

Images