Instrument for measuring endotoxin by adopting integrating sphere principle

Abstract

The utility model discloses an instrument for measuring endotoxin by adopting an integrating sphere principle, which relates to the technical field of endotoxin detection and comprises a shell and a light source irradiation hole, the computer mainboard is fixed to the front side in the shell, the heating disc is fixed to the rear side in the shell, the integrating sphere lower portions are evenly installed on the top face of the heating disc, the top faces of the integrating sphere lower portions and the bottom face of the integrating sphere upper portion are installed in a matched mode, and a light scattering cavity is formed in the integrating sphere upper portion. According to the instrument for determining endotoxin by adopting the integrating sphere principle, light sources with different wavelengths can be switched, reagent selection is not limited, each light source module can detect eight detection tubes, the light source modules adopt the integrating sphere principle, and the light source module can be used for detecting the endotoxin by adopting the integrating sphere principle. And the luminous fluxes irradiating the eight detection tubes are the same, so that the system error of the experiment is reduced, and a trace detection method of a photometric determination method can be realized.

Description

Technical Field

[0001] The utility model relates to the technical field of endotoxin detection, in particular to an instrument for measuring endotoxin by adopting the integrating sphere principle. Background Art

[0002] Bacterial endotoxin inspection is an important quality indicator to ensure the safety of injections. The traditional method for bacterial endotoxin detection is photometry. The sample is irradiated with a specific wavelength and the absorbance change of the sample is detected to determine the endotoxin content of the sample. The disadvantages of the existing detection method are: 1. Because there is only a fixed wavelength light source, the selection of reagents is limited during the test and detection process; 2. After long-term use, the attenuation of each light source is different, which affects the experimental results; 3. There are many light sources, the experimental system error is large, and the uniformity of the experimental results is not good. The traditional bacterial endotoxin meter is a single light source for a single test tube, and multiple groups of test tubes are tested simultaneously. Due to the deviation of the light source wavelength, the absorbance of the test tube measured in the experiment will be affected, and the measured experimental results will have errors. In addition, after long-term use, the attenuation of the light source is inconsistent, the wavelength deviation is greater, and the error of the experimental results increases. For this reason, we propose an instrument for measuring endotoxins using the integrating sphere principle. Utility Model Content

[0003] The technical problem to be solved by the utility model is to overcome the existing defects and provide an instrument for determining endotoxins using the integrating sphere principle. The instrument can switch light sources of different wavelengths and has no limitation in reagent selection. Each light source module can detect eight detection tubes. The light source module adopts the integrating sphere principle and the light flux irradiating the eight detection tubes is the same, which reduces the systematic error of the experiment and realizes the micro-detection method of the photometric method, which can effectively solve the problems in the background technology.

[0004] To achieve the above-mentioned purpose, the utility model provides the following technical solutions: an instrument for measuring endotoxins using the integrating sphere principle, comprising a housing and a light source irradiation hole;

[0005] Shell: A computer motherboard is fixed on the front side of the interior, the heating plate is fixed on the rear side of the interior of the shell, the lower part of the integrating sphere is evenly installed on the top surface of the heating plate, the top surface of the lower part of the integrating sphere is matched with the bottom surface of the upper part of the integrating sphere, a light scattering cavity is provided inside the upper part of the integrating sphere, the light source irradiation hole is provided in the middle of the upper part of the integrating sphere, the bottom end of the light source irradiation hole is connected with the light scattering cavity, the outer side of the upper part of the integrating sphere is evenly provided with window holes, the top surface of the upper part of the integrating sphere is evenly provided with detection tube placement positions, and the window holes pass through the detection tube placement positions and are connected with the light scattering cavity;

[0006] It also includes a lamp cover, which is installed on the top surface of the integrating sphere. The input end of the heating plate is electrically connected to the output end of the computer motherboard, and the input end of the computer motherboard is electrically connected to the output end of the external power supply.

[0007] The light source on the lamp cover enters the light scattering cavity through the light source irradiation hole, and irradiates the light to the detection tube inside the detection tube placement position through the window hole. Then the light reaches the detection sensor to obtain the experimental result. In this way, multiple groups of detection tubes can be tested at the same time, and the heating plate can control the temperature of the experiment to achieve better detection effect.

[0008] Furthermore, it also includes a temperature control board, which is installed on the inner bottom surface of the shell, the output end of the temperature control board is electrically connected to the input end of the heating plate, and the input end of the temperature control board is electrically connected to the output end of the computer motherboard. The set temperature control board can facilitate personnel to adjust the temperature of the heating plate to meet different usage requirements.

[0009] Furthermore, it also includes an upper cover and a partition, wherein the upper cover is hinged on the top surface of the shell, and the partition is plugged into the inside of the shell. The arrangement of the upper cover and the partition can effectively insulate the instrument.

[0010] Furthermore, it also includes heat dissipation holes, which are evenly opened on the left and right sides of the shell. The opened heat dissipation holes can quickly dissipate the heat inside the shell to prevent heat accumulation from causing overheating and damage to electrical components.

[0011] Furthermore, it also includes a highly reflective coating, which is evenly coated on the inner wall of the light scattering cavity. The coated highly reflective coating can effectively improve the efficiency of light scattering.

[0012] Compared with the prior art, the beneficial effects of the utility model are: the instrument for determining endotoxins using the integrating sphere principle has the following advantages:

[0013] 1. The light source irradiation method of this instrument adopts the principle of integrating sphere, and the minimum diameter of the window hole can reach 0.8MM, realizing the micro-detection of photometric detection method;

[0014] 2. The light source of this instrument adopts the principle of integrating sphere. The detection tube in each integrating sphere receives equal light, which reduces the system error of the experimental instrument.

[0015] 3. This instrument can replace light sources of any wavelength, which is convenient for replacement. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 It is a schematic diagram of the structure of the utility model;

[0017] Figure 2This is a schematic diagram of the internal structure of the housing of the utility model;

[0018] Figure 3 It is a schematic diagram of the top view structure of the utility model;

[0019] Figure 4 This is a schematic diagram of the structure of the integrating sphere of the utility model;

[0020] Figure 5 This is a schematic diagram of the upper structure of the integrating sphere of the utility model;

[0021] Figure 6 This is a schematic diagram of the upper cross-sectional structure of the integrating sphere of the utility model;

[0022] Figure 7 The figure is a schematic diagram of the upper bottom surface structure of the integrating sphere of the utility model.

[0023] In the figure: 1 outer shell, 2 upper cover, 3 computer main board, 4 heating plate, 5 lower part of integrating sphere, 6 upper part of integrating sphere, 7 lamp cover, 8 light scattering cavity, 9 light source irradiation hole, 10 detection tube placement position, 11 window hole, 12 temperature control board, 13 partition, 14 heat dissipation hole, 15 high reflective coating. DETAILED DESCRIPTION

[0024] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0025] See also Figure 1-7 , This embodiment provides a technical solution: an instrument for measuring endotoxins using the integrating sphere principle, comprising a housing 1 and a light source irradiation hole 9;

[0026] Shell 1: A computer motherboard 3 is fixed to the front side of the interior, a heating plate 4 is fixed to the rear side of the interior of the shell 1, an integrating sphere lower part 5 is evenly installed on the top surface of the heating plate 4, the top surface of the integrating sphere lower part 5 is matched with the bottom surface of the integrating sphere upper part 6, a light scattering cavity 8 is provided inside the integrating sphere upper part 6, a light source irradiation hole 9 is provided in the middle of the top surface of the integrating sphere upper part 6, the bottom end of the light source irradiation hole 9 is connected to the light scattering cavity 8, window holes 11 are evenly provided on the outside of the integrating sphere upper part 6, a detection tube placement position 10 is evenly provided on the top surface of the integrating sphere upper part 6, and the window hole 11 passes through the detection tube placement position 10 and is connected to the light scattering cavity 8;

[0027] Among them, it also includes a lamp cover 7, which is installed on the top surface of the integrating sphere upper part 6, the input end of the heating plate 4 is electrically connected to the output end of the computer mainboard 3, and the input end of the computer mainboard 3 is electrically connected to the output end of the external power supply. The light source on the lamp cover 7 enters the interior of the light scattering cavity 8 through the light source irradiation hole 9, and the light is irradiated on the detection tube inside the detection tube placement position 10 through the window hole 11, and then the light reaches the detection sensor to obtain the experimental result, so that multiple groups of detection tubes can be detected at the same time, and the heating plate 4 can control the temperature of the experiment to achieve a better detection effect. It also includes a temperature control board 12, which is installed on the inner bottom surface of the shell 1, and the output end of the temperature control board 12 is electrically connected to the input end of the heating plate 4, and the input of the temperature control board 12 The end is electrically connected to the output end of the computer motherboard 3. The set temperature control board 12 can facilitate personnel to adjust the temperature of the heating plate 4 to meet different usage requirements. It also includes an upper cover 2 and a partition 13. The upper cover 2 is hinged on the top surface of the shell 1, and the partition 13 is plugged into the inside of the shell 1. The setting of the upper cover 2 and the partition 13 can effectively insulate the instrument. It also includes heat dissipation holes 4. The heat dissipation holes 4 are evenly opened on the left and right sides of the shell 1. The opened heat dissipation holes 4 can quickly dissipate the heat inside the shell 1 to prevent heat accumulation from causing overheating and damage to electrical components. It also includes a highly reflective coating 15. The highly reflective coating 15 is evenly coated on the inner wall of the light scattering cavity 8. The coated high-reflective coating 15 can effectively improve the efficiency of light scattering.

[0028] The working principle of an instrument for determining endotoxin using the integrating sphere principle provided by the utility model is as follows: first, the heating plate 4 is started and the test temperature is maintained at 37±0.2° in conjunction with the temperature control plate 12, and the detection tube to be detected is inserted into the detection tube placement position 10, and the light source on the lamp cover 7 enters the light scattering cavity 8 through the light source irradiation hole 9, and the light is irradiated to the detection tube inside the detection tube placement position 10 through the window hole 11. The highly reflective coating 15 applied can effectively improve the efficiency of light scattering, and then the light reaches the detection sensor to obtain the experimental results, so that multiple groups of detection tubes can be detected at the same time, and the detection tubes inside each integrating sphere are equally illuminated, thereby reducing the system error of the experimental instrument.

[0029] The above description is only an embodiment of the present invention, and does not limit the patent scope of the present invention. Any equivalent structure or equivalent process transformation made by using the contents of the specification and drawings of the present invention, or directly or indirectly applied in other related technical fields, are also included in the patent protection scope of the present invention.

Claims

1. An instrument for determining endotoxin using the integrating sphere principle, characterized in that: It comprises a housing (1) and a light source irradiation hole (9); The housing (1) has a computer motherboard (3) fixed on the front side of the interior, a heating plate (4) fixed on the rear side of the interior of the housing (1), an integrating sphere lower part (5) evenly mounted on the top surface of the heating plate (4), the top surface of the integrating sphere lower part (5) being mounted in coordination with the bottom surface of the integrating sphere upper part (6), a light scattering cavity (8) being provided inside the integrating sphere upper part (6), a light source irradiation hole (9) being provided in the middle of the top surface of the integrating sphere upper part (6), the bottom end of the light source irradiation hole (9) being connected to the light scattering cavity (8), a window hole (11) being evenly provided on the outside of the integrating sphere upper part (6), a detection tube placement position (10) being evenly provided on the top surface of the integrating sphere upper part (6), the window hole (11) passing through the detection tube placement position (10) being connected to the light scattering cavity (8); It also includes a lamp cover (7), which is installed on the top surface of the upper part (6) of the integrating sphere. The input end of the heating plate (4) is electrically connected to the output end of the computer motherboard (3), and the input end of the computer motherboard (3) is electrically connected to the output end of an external power supply.

2. The instrument for determining endotoxins using the integrating sphere principle according to claim 1, characterized in that: It also includes a temperature control board (12), which is installed on the inner bottom surface of the housing (1), the output end of the temperature control board (12) is electrically connected to the input end of the heating plate (4), and the input end of the temperature control board (12) is electrically connected to the output end of the computer motherboard (3).

3. The instrument for determining endotoxins using the integrating sphere principle according to claim 1, characterized in that: It also comprises an upper cover (2) and a partition (13), wherein the upper cover (2) is hinged on the top surface of the outer shell (1), and the partition (13) is inserted into the interior of the outer shell (1).

4. The instrument for determining endotoxin using the integrating sphere principle according to claim 1, characterized in that: It also comprises heat dissipation holes (14), and the heat dissipation holes (14) are evenly arranged on the left and right sides of the housing (1).

5. The instrument for determining endotoxin using the integrating sphere principle according to claim 1, characterized in that: It also includes a highly reflective paint (15), wherein the highly reflective paint (15) is evenly coated on the inner wall of the light scattering cavity (8).

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