Western blot hybridization reaction device

Abstract

The utility model belongs to the technical field of western blot reaction equipment, and particularly relates to a western blot hybridization reaction device which comprises a reaction device body, a containing cavity is formed in the side end of the reaction device body, a threaded rod is installed in the containing cavity, a movable block is installed at the side end of the threaded rod, and a hydraulic telescopic rod is fixedly installed at the upper end of the movable block. A placement plate is fixedly mounted at the upper end of the hydraulic telescopic rod, a placement groove of a circular structure is formed in the upper end of the placement plate, four mounting grooves are symmetrically formed in the periphery of the placement groove, and a clamping assembly is mounted in each mounting groove; each clamping assembly comprises a spring, a telescopic assembly, a clamping plate and an anti-skid gasket, one end of each spring is located in the corresponding containing groove and fixedly connected with the inner wall of the corresponding containing plate, the other end of each spring is fixedly connected with the corresponding clamping plate, the telescopic assemblies are located on the inner sides of the springs, and the two ends of each telescopic assembly are fixedly connected with the corresponding containing plate and the corresponding clamping plate correspondingly. The anti-skid gaskets are fixedly installed at the ends, away from the springs, of the clamping plates.

Description

Technical Field

[0001] The utility model belongs to the technical field of protein blot reaction equipment, in particular to a protein blot hybridization reaction device. Background Art

[0002] The protein blot is a device for detecting the characteristics, expression and distribution of immune proteins. It uses the principle of protein blot to sequentially react a nitrocellulose membrane printed with protein strips (equivalent to a solid phase carrier membrane strip coated with antigens) with specific antibodies and enzyme-labeled secondary antibodies, and then add an enzyme reaction substrate that can form an insoluble colorant to stain the zone. Then, a camera is used to take a picture of the color-developed membrane strip, and the picture is uploaded to the software for grayscale recognition and analysis to make qualitative and semi-quantitative interpretation of the results. The protein blot is mainly used for the confirmation and detection of various pathogens such as HIV, HCV, HTLV, HP, as well as the detection of autoantibodies (ENA) related to rheumatism, atopic allergen blot detection, and antigen spectrum blot detection.

[0003] The structure of the existing protein blotting instrument is too simple. During the detection process, the instrument containing the sample is directly placed in the detection area for detection. Since different instruments are of different sizes, if the instrument is not stably fixed, it is easy for the instrument to vibrate and slip, affecting the normal detection of the protein blotting instrument. Utility Model Content

[0004] In view of the deficiencies of the prior art, the present invention aims to provide a protein blotting hybridization reaction device.

[0005] The purpose of this disclosure can be achieved through the following technical solutions:

[0006] A protein blotting hybridization reaction device, comprising:

[0007] A reaction device body, wherein a receiving cavity is provided at the side end of the reaction device body, a threaded rod is installed inside the receiving cavity, a moving block is installed at the side end of the threaded rod, a hydraulic telescopic rod is fixedly installed at the upper end of the moving block, a placement plate is fixedly installed at the upper end of the hydraulic telescopic rod, a placement groove of a circular structure is provided at the upper end of the placement plate, four installation grooves are symmetrically provided around the placement groove, and a clamping assembly is installed in each installation groove;

[0008] The clamping assembly includes a spring, a telescopic assembly, a clamping plate, and an anti-slip gasket. One end of the spring is located in the placement groove and fixedly connected to the inner wall of the placement plate. The other end of the spring is fixedly connected to the clamping plate. The telescopic assembly is located on the inner side of the spring, and the two ends of the telescopic assembly are respectively fixedly connected to the placement plate and the clamping plate. The anti-slip gasket is fixedly installed on the end of the clamping plate away from the spring.

[0009] Furthermore, the threaded rod is rotatably connected to the reaction device body, a driving motor is fixedly installed on the side end of the reaction device body, the driving motor is fixedly connected to one end of the threaded rod, and the moving block is threadedly connected to the threaded rod.

[0010] Furthermore, the instrument for holding the sample is placed in a placement groove of the placement plate, and the anti-slip pads of the four clamping assemblies are in contact with the side walls of the instrument.

[0011] Furthermore, the telescopic assembly includes a first telescopic rod and a second telescopic rod, the first telescopic rod is hollow inside, the inner diameter of the first telescopic rod is equal to the outer diameter of the second telescopic rod, two sliding grooves are provided on the inner wall of the first telescopic rod, and sliders are fixedly installed on both side ends of the second telescopic rod, the sliders are adapted to the sliding grooves, and the sliders are located in the sliding grooves.

[0012] Furthermore, a vibration plate is installed at the bottom of the placement plate located in the placement groove, a vibration motor is installed inside the placement plate, and the vibration motor is electrically connected to the vibration plate.

[0013] Furthermore, a through groove is provided at the upper end of the reaction device body, and two slots are provided at the bottom of the through groove. A mounting plate is installed at the through groove, and two plug blocks are fixedly installed at the bottom of the mounting plate, and the plug blocks are adapted to the slots.

[0014] Furthermore, a plurality of medicine grooves are provided at the upper end of the mounting plate, a medicine bottle is placed in each of the medicine grooves, a medicine outlet of the medicine bottle passes through the mounting plate vertically downward, and the device is located directly below the medicine bottle.

[0015] Furthermore, a limiting groove is provided on the inner wall of the reaction device body, a support rod is fixedly installed on one side of the moving block close to the limiting groove, a limiting block is fixedly installed on the side end of the support rod, and the limiting block is adapted to the limiting groove.

[0016] The nouns, conjunctions or adjectives involved in the above technical solution are explained as follows:

[0017] Fixed connection: refers to a connection in which parts or components are fixed without any relative movement. It is divided into detachable connection and non-detachable connection.

[0018] (1) Removable connection: The parts are fixed together by screws, splines, wedge pins, etc. This connection method can be disassembled for maintenance without damaging the parts. However, the specifications of the connectors used must be correct (such as the length of the bolts, keys, and wedge pins) and they must be properly tightened.

[0019] (2) Non-detachable connection: mainly refers to welding, riveting and tenoning. Since forging, sawing or oxygen cutting are required for disassembly during maintenance or replacement, spare parts generally cannot be reused. At the same time, attention should be paid to process quality, technical inspection and remedial measures (such as calibration, polishing, etc.) during connection.

[0020] The beneficial effects of the present disclosure are as follows: when the device is used to carry out protein blotting reaction, the instrument containing the sample to be tested and the detection agent is placed in the placement groove at the upper end of the placement plate, and the instrument is clamped and limited by four clamping components located inside the placement groove. The cooperation of the spring and the telescopic component in the clamping component enables the device to clamp instruments of different sizes, so that the instrument is confined in the placement groove, which can effectively prevent the instrument from slipping during vibration. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, for ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0022] Figure 1 It is a schematic diagram of the overall structure of the protein blotting hybridization reaction device of the embodiment of the utility model;

[0023] Figure 2 It is a cross-sectional view of the entire protein blotting hybridization reaction device of the embodiment of the utility model;

[0024] Figure 3 It is a schematic diagram of the structure of the reaction device body of the utility model embodiment;

[0025] Figure 4 It is a structural schematic diagram of a moving block and a placement plate in an embodiment of the utility model;

[0026] Figure 5 is a cross-sectional view of the embodiment of the utility model when the device is placed in the placement plate;

[0027] Figure 6 It is a schematic diagram of the structure when the clamping assembly of the embodiment of the utility model is installed in the placement plate;

[0028] Figure 7 This is a schematic diagram of the structure of the placement plate of the embodiment of the utility model;

[0029] Figure 8 It is a structural schematic diagram of the clamping assembly of an embodiment of the utility model;

[0030] Fig. 9 is a cross-sectional view of a telescopic assembly according to an embodiment of the utility model;

[0031] Fig.10 This is a schematic diagram of the structure of the first telescopic rod of an embodiment of the utility model;

[0032] Fig.11 It is a schematic diagram of the structure of the second telescopic rod of the embodiment of the utility model;

[0033] Fig.12 It is a structural schematic diagram of a mounting plate according to an embodiment of the utility model.

[0034] Figure numerals: 1. reaction device body; 2. accommodating chamber; 3. threaded rod; 4. moving block; 5. hydraulic telescopic rod; 6. placement plate; 7. placement slot; 8. mounting slot; 9. clamping assembly; 10. spring; 11. telescopic assembly; 12. clamping plate; 13. anti-slip pad; 14. driving motor; 15. apparatus; 16. first telescopic rod; 17. second telescopic rod; 18. slide groove; 19. slider; 20. vibration plate; 21. vibration motor; 22. through groove; 23. slot; 24. mounting plate; 25. plug-in block; 26. medicine slot; 27. medicine bottle; 28. limit groove; 29. ​​support rod; 30. limit block. DETAILED DESCRIPTION

[0035] The following will be combined with the drawings in the embodiments of the present disclosure to clearly and completely describe the technical solutions in the embodiments of the present disclosure. Obviously, the described embodiments are only part of the embodiments of the present disclosure, rather than all the embodiments. Based on the embodiments in the present disclosure, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present disclosure.

[0036] A protein blotting hybridization reaction device, such as Figure 1-Figure 12 As shown, including:

[0037] A reaction device body 1, wherein a receiving chamber 2 is provided at the side end of the reaction device body 1, a threaded rod 3 is installed inside the receiving chamber 2, a moving block 4 is installed at the side end of the threaded rod 3, a hydraulic telescopic rod 5 is fixedly installed at the upper end of the moving block 4, a placement plate 6 is fixedly installed at the upper end of the hydraulic telescopic rod 5, a placement groove 7 with a circular structure is opened at the upper end of the placement plate 6, four installation grooves 8 are opened symmetrically around the placement groove 7, and a clamping assembly 9 is installed in each installation groove 8;

[0038] The clamping assembly 9 includes a spring 10, a telescopic assembly 11, a clamping plate 12, and an anti-slip gasket 13. One end of the spring 10 is located in the placement groove 7 and is fixedly connected to the inner wall of the placement plate 6. The other end of the spring 10 is fixedly connected to the clamping plate 12. The telescopic assembly 11 is located on the inner side of the spring 10, and the two ends of the telescopic assembly 11 are respectively fixedly connected to the placement plate 6 and the clamping plate 12. The anti-slip gasket 13 is fixedly installed on the end of the clamping plate 12 away from the spring 10.

[0039] When the device is used to perform protein blotting reaction, an instrument 15 containing a sample to be tested and a test agent is placed in the placement groove 7 at the upper end of the placement plate 6, and the instrument 15 is clamped and limited by four clamping components 9 located inside the placement groove 7. The cooperation of the spring 10 and the telescopic component 11 in the clamping component 9 enables the device to clamp instruments 15 of different sizes, so that the instrument 15 is confined in the placement groove 7, which can effectively prevent the instrument 15 from slipping during vibration.

[0040] It should be noted here that the anti-skid pad 13 is made of a flexible rubber material, and the side of the anti-skid pad 13 away from the clamping plate 12 is a rough surface.

[0041] As a preferred embodiment of the present invention, Figure 1 , Figure 2 As shown, the threaded rod 3 is rotatably connected to the reaction device body 1, and a driving motor 14 is fixedly installed on the side end of the reaction device body 1. The driving motor 14 is fixedly connected to one end of the threaded rod 3, and the moving block 4 is threadedly connected to the threaded rod 3; after the driving motor 14 is started, it will drive the threaded rod 3 to rotate, and the rotation of the threaded rod 3 will drive the moving block 4 to move linearly on the threaded rod 3, so as to adjust the position of the placement plate 6.

[0042] As a preferred embodiment of the present invention, Figure 5 As shown, the container 15 for holding the sample is placed in the placement groove 7 of the placement plate 6 , and the anti-slip pads 13 of the four clamping components 9 are in contact with the side wall of the container 15 .

[0043] As a preferred embodiment of the present invention, Figure 9-11As shown, the telescopic assembly 11 includes a first telescopic rod 16 and a second telescopic rod 17. The first telescopic rod 16 is hollow inside, and the inner diameter of the first telescopic rod 16 is equal to the outer diameter of the second telescopic rod 17. The inner wall of the first telescopic rod 16 is provided with two slide grooves 18. Both side ends of the second telescopic rod 17 are fixedly installed with sliders 19, and the sliders 19 are adapted to the slide grooves 18, and the sliders 19 are located in the slide grooves 18. The telescopic assembly 11 can be contracted as the spring 10 is compressed. The arrangement of the sliders 19 and the slide grooves 18 facilitates the second telescopic rod 17 to move inside the first telescopic rod 16 to realize the contraction of the telescopic assembly 11.

[0044] As a preferred embodiment of the present invention, Figure 5 As shown, the placement plate 6 is located at the bottom of the placement groove 7 and is equipped with a vibration plate 20, and a vibration motor 21 is installed inside the placement plate 6, and the vibration motor 21 is electrically connected to the vibration plate 20; the setting of the vibration plate 20 and the vibration motor 21 is used to oscillate the device 15 containing the sample to be tested and the detection agent, so that the sample to be tested and the detection agent are fully mixed.

[0045] As a preferred embodiment of the present invention, Figure 2 , Figure 3 as well as Fig.12 As shown, a through slot 22 is provided at the upper end of the reaction device body 1, and two slots 23 are provided at the bottom of the through slot 22 of the reaction device body 1. A mounting plate 24 is installed at the through slot 22, and two plug blocks 25 are fixedly installed at the bottom of the mounting plate 24, and the plug blocks 25 are adapted to the slots 23; a plurality of drug slots 26 are provided at the upper end of the mounting plate 24, and a drug bottle 27 is placed in each of the drug slots 26, and the drug outlet of the drug bottle 27 passes through the mounting plate 24 vertically downward, and the device 15 is located directly below the drug bottle 27. The mounting plate 24 is provided for placing the drug bottle 27. Since a variety of drugs need to be used in the process of protein blotting experiments, the multiple drugs to be used can be placed in the drug slots 26 in sequence before the experiment, and the drug outlet of the drug bottle 27 is facing downward, which is convenient for use during the experiment.

[0046] As a preferred embodiment of the present invention, Figure 1 , Figure 4As shown, a limiting groove 28 is provided on the inner wall of the reaction device body 1, and a support rod 29 is fixedly installed on one side of the moving block 4 close to the limiting groove 28, and a limiting block 30 is fixedly installed on the side end of the support rod 29, and the limiting block 30 is adapted to the limiting groove 28; the setting of the limiting block 30 and the limiting groove 28 is used to limit the moving block 4, so that when the threaded rod 3 rotates under the action of the driving motor 14, the moving block 4 threadedly connected with the threaded rod 3 can only move in a straight line along the threaded rod 3, and no shaking will occur during the movement; the setting of the support rod 29 is used to support the moving block 4.

[0047] The working principle and use process of this utility model:

[0048] When the device is used to perform protein blotting reaction, first, the medicine bottles 27 of the various detection reagents to be used are placed in the medicine slot 26 of the mounting plate 24, with the medicine outlet of the medicine bottle 27 facing downward, and then the instrument 15 containing the sample to be detected is placed in the placement slot 7 at the upper end of the placement plate 6. The four clamping components 9 in the placement slot 7 can clamp and limit the instrument 15, and then the height of the instrument 15 is adjusted by the hydraulic telescopic rod 5 so that the bottle mouth of the instrument 15 is close to the medicine outlet of the medicine bottle 27. During use, the threaded rod 3 can be driven to rotate by the driving motor 14, thereby driving the moving block 4 to move in a straight line, so as to facilitate the movement of the instrument 15 to the bottom of different medicine bottles 27, so as to facilitate the addition of medicines. After the addition of medicines is completed, the vibration plate 20 is driven by the vibration motor 21 to oscillate the instrument 15 containing the sample to be detected and the detection reagent, so that the sample to be detected and the detection reagent are fully mixed.

[0049] In the description of this specification, the description with reference to the terms "one embodiment", "example", "specific example", etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present disclosure. In this specification, the schematic representation of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any one or more embodiments or examples in a suitable manner.

[0050] The above shows and describes the basic principles, main features and advantages of the present disclosure. Those skilled in the art should understand that the present disclosure is not limited by the above embodiments, and the above embodiments and descriptions are only for explaining the principles of the present disclosure. Without departing from the spirit and scope of the present disclosure, the present disclosure may have various changes and improvements, and these changes and improvements fall within the scope of the present disclosure to be protected.

Claims

1. A protein blotting hybridization reaction device, characterized in that: include: A reaction device body (1), wherein a receiving chamber (2) is provided at a side end of the reaction device body (1), a threaded rod (3) is installed inside the receiving chamber (2), a moving block (4) is installed at the side end of the threaded rod (3), a hydraulic telescopic rod (5) is fixedly installed at the upper end of the moving block (4), a placement plate (6) is fixedly installed at the upper end of the hydraulic telescopic rod (5), a placement groove (7) with a circular structure is provided at the upper end of the placement plate (6), four installation grooves (8) are symmetrically provided around the placement groove (7), and a clamping assembly (9) is installed in each installation groove (8); The clamping assembly (9) comprises a spring (10), a telescopic assembly (11), a clamping plate (12), and an anti-slip pad (13); one end of the spring (10) is located in the placement groove (7) and is fixedly connected to the inner wall of the placement plate (6); the other end of the spring (10) is fixedly connected to the clamping plate (12); the telescopic assembly (11) is located on the inner side of the spring (10), and the two ends of the telescopic assembly (11) are respectively fixedly connected to the placement plate (6) and the clamping plate (12); the anti-slip pad (13) is fixedly mounted on one end of the clamping plate (12) away from the spring (10).

2. A protein blot hybridization reaction device according to claim 1, characterized in that: The threaded rod (3) is rotatably connected to the reaction device body (1); a driving motor (14) is fixedly mounted on the side end of the reaction device body (1); the driving motor (14) is fixedly connected to one end of the threaded rod (3); and the moving block (4) is threadably connected to the threaded rod (3).

3. A protein blot hybridization reaction device according to claim 2, characterized in that: The device (15) for holding the sample is placed in the placement groove (7) of the placement plate (6), and the anti-slip pads (13) of the four clamping components (9) are in contact with the side wall of the device (15).

4. A protein blot hybridization reaction device according to claim 3, characterized in that: The telescopic assembly (11) comprises a first telescopic rod (16) and a second telescopic rod (17); the first telescopic rod (16) is hollow inside, the inner diameter of the first telescopic rod (16) is equal to the outer diameter of the second telescopic rod (17); two slide grooves (18) are provided on the inner wall of the first telescopic rod (16); sliders (19) are fixedly mounted on both side ends of the second telescopic rod (17); the sliders (19) are matched with the slide grooves (18), and the sliders (19) are located in the slide grooves (18).

5. A protein blot hybridization reaction device according to claim 4, characterized in that: The placement plate (6) is located at the bottom of the placement groove (7) and is equipped with a vibration plate (20). A vibration motor (21) is installed inside the placement plate (6), and the vibration motor (21) is electrically connected to the vibration plate (20).

6. A protein blot hybridization reaction device according to claim 5, characterized in that: A through slot (22) is provided at the upper end of the reaction device body (1); two slots (23) are provided at the bottom of the through slot (22) of the reaction device body (1); a mounting plate (24) is installed at the through slot (22); two insert blocks (25) are fixedly installed at the bottom of the mounting plate (24); the insert blocks (25) are adapted to fit the slots (23).

7. A protein blot hybridization reaction device according to claim 6, characterized in that: A plurality of medicine grooves (26) are provided at the upper end of the mounting plate (24), and a medicine bottle (27) is placed in each of the medicine grooves (26). The medicine outlet of the medicine bottle (27) passes through the mounting plate (24) vertically downward, and the device (15) is located directly below the medicine bottle (27).

8. A protein blot hybridization reaction device according to claim 7, characterized in that: A limiting groove (28) is provided on the inner wall of the reaction device body (1); a support rod (29) is fixedly installed on one side of the moving block (4) close to the limiting groove (28); a limiting block (30) is fixedly installed on the side end of the support rod (29); and the limiting block (30) is adapted to the limiting groove (28).

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