Portable constant-temperature in-vitro PCR (Polymerase Chain Reaction) amplification detection instrument

Abstract

The utility model discloses a portable constant temperature in vitro PCR amplification detection instrument, including carrying case and PCR amplification detection instrument main part, the inside of carrying case is provided with the accommodation chamber, the both ends of accommodation chamber are respectively provided with the sample placing chamber and the waste sample placing chamber, and the sample placing chamber and the waste sample placing chamber are both provided with the placing subassembly, and the placing subassembly is provided with the PCR amplification detection instrument main part. The interior of the storage cavity is connected with the PCR amplification detector main body through a mounting assembly, the mounting assembly is connected with the carrying box through a positioning assembly, and a cover plate is arranged at the top end of the carrying box through a buckle. According to the PCR amplification detector box, the PCR amplification detector body can be conveniently moved out and stored, the PCR amplification detector body can be conveniently moved out of the box body during detection use, follow-up maintenance and the like, detection and other operations are facilitated, the PCR amplification detector box can be stored and fixed after use, the storage effect is ensured, the whole PCR amplification detector body can be conveniently carried and taken, and the PCR amplification detector box is more convenient to use.

Description

Technical Field

[0001] The utility model relates to the technical field of nucleic acid sample amplification and detection, in particular to a portable constant-temperature in vitro PCR amplification detection instrument. Background Art

[0002] Polymerase chain reaction amplification technology is the most commonly used in vitro amplification technology in modern molecular biology. It can amplify a small amount of nucleic acid in the sample to be tested in vitro to meet the detection requirements. At present, this technology has been widely used in the fields of disease diagnosis, pathogen detection and forensic identification. However, PCR technology requires a strict temperature control process to achieve the cycle of DNA double-strand unwinding, primer hybridization, new DNA chain synthesis, etc., and it has high requirements on the precision of instruments and equipment. When used for detection, in order to improve convenience and speed, it is necessary to perform detection and analysis outdoors. Therefore, a portable constant temperature in vitro PCR amplification detection instrument is proposed.

[0003] A Chinese patent publication number CN214193272U disclosed a portable constant temperature in vitro PCR amplification detection instrument, including a test kit, wherein the test kit includes a metal bathtub, a test box and a centrifuge tube, and the metal bathtub, a test box and a centrifuge tube are arranged in the test kit in an upper and lower distribution. The test kit in the utility model allows all steps other than the sample addition step to be performed in a relatively closed independent environment, thereby avoiding cross contamination between samples to the greatest extent. At the same time, the test kit can not only be conveniently carried, but also achieve multiple effects such as ensuring that the sample detection process is not disturbed by the environment, reducing false positive detection, and avoiding sample leakage.

[0004] However, the existing portable constant temperature in vitro PCR amplification detection instrument has certain defects in actual use. Although it is convenient to carry, it is located inside the box when in use, and it is not convenient to remove it for detection operation. It is also not convenient to perform maintenance operations when a fault occurs. At the same time, the samples after detection are not convenient to collect and carry, and it is not convenient to process them. Utility Model Content

[0005] The purpose of the utility model is to provide a portable constant temperature in vitro PCR amplification detection instrument to solve the problems raised in the above background technology.

[0006] To achieve the above-mentioned purpose, the utility model provides the following technical solutions: a portable constant temperature in vitro PCR amplification detection instrument, comprising a carrying box and a PCR amplification detection instrument main body, a storage cavity is arranged inside the carrying box, a sample placement cavity and a waste sample placement cavity are respectively arranged at the two ends of the storage cavity, and placement components are arranged in the sample placement cavity and the waste sample placement cavity, the storage cavity is connected to the PCR amplification detection instrument main body through an installation component, and the installation component and the carrying box are connected through a positioning component, and a cover plate is arranged on the top of the carrying box through a tower buckle.

[0007] Preferably, in order to facilitate the placement of tools and sampling and other operations, the placement component includes partitions equidistantly arranged in the sample placement cavity, and a card pad is provided at the bottom end of the sample placement cavity. The structure of the placement component in the waste sample placement cavity is the same as the structure of the placement component in the sample placement cavity.

[0008] Preferably, in order to facilitate the movement of the PCR amplification detector body, it can be conveniently removed when in use and stored when not in use, the installation component includes a mounting base plate fixedly arranged at the bottom end of the PCR amplification detector body, a pull plate is fixedly arranged on the side wall of the mounting base plate, and the carrying box is provided with a through hole adapted to the pull plate.

[0009] Preferably, in order to facilitate the pulling operation, and to facilitate the pulling out and storage operations, the installation component also includes a mounting slide symmetrically arranged at the bottom end of the installation base plate, and a mounting groove matching the mounting slide is opened at the bottom end of the storage cavity.

[0010] Preferably, in order to position and fix the device when stored to avoid sliding, the positioning assembly includes a rotating rod equidistantly arranged on the pull plate, a positioning plate is fixed to one end of the rotating rod away from the main body of the PCR amplification detector, and a positioning groove matching the positioning plate is opened on the side panel of the carrying box.

[0011] Preferably, in order to assist in fixation and ensure the positioning effect, while also facilitating subsequent pulling-out operations, fixed magnetic suctions are provided inside the positioning groove and on the side plates of the positioning plate. The two fixed magnetic suctions have opposite magnetic properties, and a limit plate is fixedly provided at the end of the rotating rod away from the positioning plate.

[0012] Preferably, in order to facilitate carrying and positioning to avoid hard contact, a handle is provided in the middle of the top of the cover plate, and a sponge pad is provided at the bottom of the cover plate, and the sponge pads are respectively matched with the storage cavity, the sample placement cavity and the waste sample placement cavity.

[0013] Compared with the prior art, the beneficial effects of the utility model are:

[0014] (1) The main body of the PCR amplification detector can be conveniently removed and stored by means of the provided installation component and the positioning component. It can be conveniently removed from the outside of the box during testing and subsequent maintenance, which is convenient for testing and other operations. It can also be stored and fixed after use to ensure the storage effect, making it convenient to carry and take the whole body, which is more convenient;

[0015] (2) The placement component can not only place and take the sampling and testing tools, which is convenient for sampling and other operations, but also temporarily store the samples used for sampling and testing, which is convenient for unified collection and storage, and subsequent unified processing, avoiding pollution to the environment, etc. It is also convenient for comparative analysis when abnormalities are detected in the subsequent detection, which is convenient for re-testing, etc., thereby improving the accuracy of the detection. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 This is a schematic diagram of the structure of a portable constant temperature in vitro PCR amplification detection instrument proposed by the utility model when it is opened;

[0017] Figure 2 This is a partial structural schematic diagram of a portable constant temperature in vitro PCR amplification detection instrument proposed by the utility model;

[0018] Figure 3 This is a schematic diagram of the structure of the installation components and the main body of the PCR amplification detection instrument in a portable constant temperature in vitro PCR amplification detection instrument proposed by the utility model;

[0019] Figure 4 This is a schematic diagram of the structure of a carrying box in a portable constant temperature in vitro PCR amplification detection instrument proposed by the utility model;

[0020] Figure 5 The utility model provides a schematic diagram of the structure of a box cover in a portable constant temperature in vitro PCR amplification detection instrument.

[0021] In the figure: 1. Carrying box; 2. Cover plate; 3. Pull plate; 4. PCR amplification detector body; 5. Tower buckle; 6. Handle; 7. Installation bottom plate; 8. Storage cavity; 9. Partition plate; 10. Sample placement cavity; 11. Waste sample placement cavity; 12. Card pad; 13. Positioning plate; 14. Installation slide groove; 15. Installation slide plate; 16. Limiting plate; 17. Fixed magnet; 18. Positioning groove; 19. Sponge pad; 20. Turn rod. DETAILED DESCRIPTION

[0022] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0023] See also Figure 1-Figure 5The utility model provides an embodiment: a portable constant temperature in vitro PCR amplification detection instrument, including a PCR amplification detection instrument body 4 for in vitro amplification of nucleic acids for detection, the interior of which is mainly composed of a nucleic acid amplification module, a detection module, a heating constant temperature module, a power supply module, etc., which are convenient for amplification and detection operations. The outdoor portable detection principle can use colloidal gold test strips to measure nucleic acids (its principle nucleic acid test strip detection technology mainly uses the protein colloidal gold principle, mainly in the PCR upstream and downstream primers are modified with fluorescein isothiocyanate and biotin; the nucleic acid test strips on The colloidal gold and the detection line are marked with streptavidin and anti-fluorescein antibodies respectively. When the amplified product is mixed with the extension solution and then spotted on the test strip, when there is an amplified product, the biotin modified on the product combines with the colloidal gold of fluorescein isothiocyanate modified on the gold label. When it reaches the detection line, the anti-fluorescein antibody on the line captures the product labeled with fluorescein, thereby developing color; the excess colloidal gold combines with biotin when it reaches the quality control line to develop color) This is a relatively mature technology and will not be elaborated here. The carrying box 1 for conveniently placing the PCR amplification detector main body 4 can facilitate its placement and carrying operations, thereby improving portability.

[0024] See also Figure 1-Figure 5 In order to facilitate carrying and subsequent testing and other pull-out uses, the bottom end of the PCR amplification detector body 4 can be bolted with a mounting base plate 7 of the mounting assembly, and a pull plate 3 is vertically welded on its side plate, which can be convenient for pulling and pushing operations. A through hole adapted to the pull plate 3 is opened on the side wall of the carrying box 1, which is convenient for closing and opening operations, and two groups of mounting slide plates 15 are welded at the bottom end of the mounting base plate 7, and a mounting slide groove 14 is opened at the bottom end of the storage cavity 8 (that is, the bottom end of the carrying box 1) for facilitating the sliding of the mounting slide plate 15. For better switch operation, a handle is rotatably installed in the middle of the outer wall of the pull plate 3, and in order to ensure firmness during storage and avoid installation In order to prevent the bottom plate 7 from sliding as a whole and facilitate the subsequent pulling-out operation, a rotating rod 20 of the positioning assembly is equidistantly arranged on the pull plate 3 for sliding. A limiting plate 16 is welded at one end of the rotating rod 20 close to the PCR amplification detector body 4, and a positioning plate 13 is welded at the other end, which can facilitate sliding and rotating operations on the pull plate 3 and avoid falling off. Positioning grooves 18 (four groups or other groups are acceptable to ensure the positioning effect) that are compatible with the positioning plate 13 are provided on the side panel of the carrying box 1. In order to ensure the firmness of the positioning, fixed magnetic suction 17 is bonded to the positioning plate 13 and the positioning groove 18. The two groups of magnetic properties are opposite and can be attracted and fixed to each other to ensure firmness.

[0025] See also Figure 1-Figure 5In order to facilitate the placement of sampling tubes and the PCR amplification detector body 4, the interior of the carrying box 1 is divided into a storage chamber 8, a sample placement chamber 10 and a waste sample placement chamber 11 by a baffle, which can facilitate sampling, testing and temporary placement of samples after testing when used outdoors, and facilitate subsequent unified processing operations. The sample placement chamber 10 and the waste sample placement chamber 11 are welded with partitions 9 for placing components (which can be divided into three, four or five groups, etc., according to actual conditions), and the inside is bonded with card pads 12 (the material can be flexible materials such as rubber or sponge). In order to provide protection for the whole and facilitate opening operation, the top of the carrying box 1 is installed with a cover plate 2 through a tower buckle 5 (the number of tower buckles 5 can be four groups), and a handle 6 for carrying and extracting the whole is installed in the middle of the cover plate 2, and a sponge pad 19 is bonded to the bottom, which corresponds to the storage chamber 8, the sample placement chamber 10 and the waste sample placement chamber 11 one by one.

[0026] Working principle: When the utility model is in use, the cover plate 2 and the carrying case 1 are fixed by the tower buckle 5, and the overall carrying and taking operation can be facilitated by the handle 6, which is easy to carry. When it is needed, the tower buckle 5 is pulled to open it, and then the cover plate 2 can be removed, and then the positioning plate 13 is pulled to take it out from the positioning groove 18, the two fixed magnetic suctions 17 are separated by force, the rotating rod 20 slides on the pulling plate 3, and then the pulling plate 3 is pulled, and the installation slide plate 15 under the installation bottom plate 7 can slide in the installation slide groove 14, and the PCR amplification detector main body can be moved. The body 4 is moved out of the storage chamber 8, which can facilitate subsequent sampling, amplification and detection operations. After the detection is completed, the sampling bottles can be temporarily placed in the waste sample placement chamber 11 and taken away for subsequent collection and processing to avoid pollution to the environment. After use, it can be pushed into the storage chamber 8 by pushing the pull plate 3, and then by pushing the positioning plate 13, it enters the positioning groove 18 under the action of the attraction of the fixed magnetic suction 17, and then the cover plate 2 is covered and fixed, which can facilitate the storage and protection of the whole and facilitate the carrying operation.

[0027] It is obvious to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above, and that the present invention can be implemented in other specific forms without departing from the spirit or essential features of the present invention. Therefore, the embodiments should be regarded as exemplary and non-restrictive from any point of view, and the scope of the present invention is defined by the appended claims rather than the above description, and it is intended that all changes falling within the meaning and scope of the equivalent elements of the claims be included in the present invention. Any reference numeral in a claim should not be regarded as limiting the claim to which it relates.

Claims

1. A portable constant temperature in vitro PCR amplification detection instrument, comprising a carrying case (1) and a PCR amplification detection instrument body (4), characterized in that: The carrying case (1) is provided with a storage chamber (8) inside, and the two ends of the storage chamber (8) are respectively provided with a sample placement chamber (10) and a waste sample placement chamber (11), and the sample placement chamber (10) and the waste sample placement chamber (11) are both provided with placement components, the storage chamber (8) is connected to the PCR amplification detector body (4) via a mounting component, and the mounting component and the carrying case (1) are connected via a positioning component, the top of the carrying case (1) is provided with a cover plate (2) via a tower buckle (5), the mounting component comprises a mounting base plate (7) fixedly arranged at the bottom end of the PCR amplification detector body (4), a pull plate (3) is fixedly arranged on the side wall of the mounting base plate (7), and the carrying case (1) is provided with a through hole adapted to the pull plate (3), The installation component also includes a mounting slide plate (15) symmetrically arranged at the bottom end of the mounting base plate (7), and a mounting groove (14) matching the mounting slide plate (15) is provided at the bottom end of the storage cavity (8). The positioning component includes a rotating rod (20) equidistantly arranged on the pull plate (3), and a positioning plate (13) is fixedly provided at one end of the rotating rod (20) away from the PCR amplification detector body (4). A positioning groove (18) matching the positioning plate (13) is provided on the side plate of the carrying box (1), and fixed magnets (17) are provided inside the positioning groove (18) and on the side plate of the positioning plate (13), and the two fixed magnets (17) have opposite magnetic properties, and a limit plate (16) is fixedly provided at one end of the rotating rod (20) away from the positioning plate (13).

2. A portable constant temperature in vitro PCR amplification detection instrument according to claim 1, characterized in that: The placement component comprises partitions (9) equidistantly arranged in the sample placement cavity (10), and a card pad (12) is provided at the bottom end of the interior of the sample placement cavity (10). The structure of the placement component in the waste sample placement cavity (11) is the same as the structure of the placement component in the sample placement cavity (10).

3. A portable constant temperature in vitro PCR amplification detection instrument according to claim 2, characterized in that: A handle (6) is provided at the middle of the top end of the cover plate (2), and a sponge pad (19) is provided at the bottom end of the cover plate (2), and the sponge pad (19) is matched with the storage cavity (8), the sample placement cavity (10) and the waste sample placement cavity (11) respectively.

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