Animal microscopic imaging fixing device

Abstract

The utility model provides an animal microscopic imaging fixing device, which comprises an animal bed, a mouth support, a lifting part, a fixing part and a fixing part, wherein the mouth support is arranged on the animal bed, and the lifting part is used for lifting the mouth of an animal; the head limiting mechanism is mounted on the animal bed and used for limiting the head of an animal; the mounting seat is mounted on the animal bed, and a miniature microscope is mounted on the mounting seat. In the scheme, the head of the animal is fixed by the head limiting mechanism, so that the possibility of influencing neural activity signal recording due to animal movement is reduced, and the interference of animal movement on signal stability is reduced. And by arranging the mouth support, the head of the animal can be straightened.

Description

Technical Field

[0001] The utility model belongs to the field of animal experimental equipment, in particular to an animal microscopic imaging fixing device. Background Art

[0002] A miniature microscope (Miniscope) is a tool used to observe and record neuronal activity in the brain in real time in freely moving animals. The essence of a Miniscope is a small, lightweight microscope with a highly simplified optical-mechanical design. Its working principle is to use spontaneous or exogenous fluorescent indicators, laser excitation, and image acquisition to monitor neuronal activity. The current common practice for Miniscope imaging of animal brains is to implant a base plate into the animal's brain in advance, connect the Miniscope to the base plate through a specially designed interface, and fix it directly on the animal's head for observation. During the free movement of animals, neural signal recording is easily disturbed by animal movement, affecting imaging and experimental results. Utility Model Content

[0003] In view of the above-mentioned shortcomings of the prior art, the purpose of the present invention is to provide an animal microscopic imaging fixation device to solve the above-mentioned problems.

[0004] In order to achieve the above-mentioned purpose and other related purposes, the utility model provides an animal microscopic imaging fixation device, comprising:

[0005] Animal beds,

[0006] A mouth support, mounted on the animal bed, the mouth support being provided with a lifting portion for lifting the mouth of the animal;

[0007] A head limiting mechanism, installed on the animal bed, for limiting the head of the animal;

[0008] A mounting base is mounted on the animal bed and is used for mounting a miniature microscope.

[0009] Optionally, the mounting seat is movably connected to the animal bed so that the mounting seat can rotate on the animal bed.

[0010] Optionally, a central channel is provided on the mounting seat, the animal bed passes through the central channel, the mouth support and the head limiting mechanism are located in the central channel, and the mounting seat can be rotatably mounted on the animal bed with the central axis of the central channel as the rotation axis.

[0011] Optionally, the mounting base includes an upper body and a lower body, the animal bed is located in the upper body and the lower body, the upper body and the lower body are detachably connected, the upper body is provided with mounting holes for slidingly cooperating with the miniature microscope, and the upper body is also provided with multiple groups of locking holes for inserting locking pieces to lock the miniature microscope.

[0012] Optionally, a movable groove is provided on the lower seat body, and at least two mounting positions distributed in the width direction of the animal bed are provided in the movable groove, and connecting pillars for matching with the mounting positions are provided on the upper seat body.

[0013] Optionally, the movable groove is a waist-shaped hole, the movable groove is arranged along the width direction of the animal bed, and a blocking part for preventing the connecting pillar from detaching is arranged at the slot position in the middle of the movable groove, so that the slot width in the middle of the movable groove is smaller than the hole diameter at both ends of the movable groove.

[0014] Optionally, the connecting strut includes a large diameter section and a small diameter section, the large diameter section matches the inside of the moving groove, and the small diameter section matches the notch in the middle of the moving groove.

[0015] Optionally, a connecting hole for locking the upper base body and the miniature microscope is opened on the upper base body, and the connecting hole is opened along the axial direction of the connecting pillar and passes through the connecting pillar. A locking connector is arranged in the connecting hole, and the locking connector is threadedly matched with the connecting hole. When the connecting pillar is located in the middle of the movable groove and the locking connector is pressed against the bottom of the movable groove, the upper base body and the lower base body switch from an unlocked state to a locked state.

[0016] Optionally, the head limiting mechanism includes two groups of sliding components, and the area between the two groups of sliding components is the placement area of ​​the animal's head. The sliding component includes a sliding seat installed on the animal bed and a slider installed on the sliding seat. After the slider slides into place, it is locked and fixed on the sliding seat by a locking piece.

[0017] Optionally, the head limiting mechanism further comprises a limiting member, the limiting member is provided with a sleeve hole for limiting the jaw teeth of the animal, and the limiting member is provided with a channel for introducing gas. As described above, the animal microscopic imaging fixing device of the utility model has the following beneficial effects:

[0018] In this scheme, the mouth of the animal to be tested is placed in the mouth rest to adjust the animal's posture, and then the animal's head is fixed by a head limiting mechanism, and a miniature microscope is installed in the mounting seat, and the interface of the miniature microscope is connected to the base plate implanted in the animal's brain.

[0019] Since the animal's head is fixed by the head limiter, the possibility of the animal's movement affecting the recording of the neural activity signal is reduced, and the interference of the animal's movement on the signal stability is reduced. The animal's head can be straightened by setting a mouth rest. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 It is an axonometric view of the animal microscopic imaging fixation device in the embodiment of the utility model.

[0021] Figure 2 This is another bearing diagram of the animal microscopic imaging fixing device in the embodiment of the utility model.

[0022] Figure 3 It is an axonometric view of the mounting seat in the embodiment of the utility model.

[0023] Figure 4 It is the bearing diagram of the lower seat body in the embodiment of the utility model. DETAILED DESCRIPTION

[0024] The reference numerals in the drawings of the specification include: animal bed 1, mouth support 2, mounting seat 3, upper seat body 301, mounting hole 3011, locking hole 3012, connecting hole 3013, connecting pillar 3014, small diameter section 30141, large diameter section 30142, lower seat body 302, first arc-shaped body 3021, second arc-shaped body 3022, movable groove 3023, blocking portion 30231, arc-shaped slide rail 303, arc-shaped slide groove 304, limiting member 4, extension portion 401, sleeve hole 402, sliding assembly 5, slide seat 501, slider 502, base 6, first mounting protrusion 601, second mounting protrusion 602, placement groove 6021, plug block 603.

[0025] The following is a description of the implementation of the present invention by means of specific embodiments. People familiar with the art can easily understand other advantages and effects of the present invention from the contents disclosed in this specification.

[0026] The utility model provides an animal microscopic imaging fixing device, such as Figures 1 to 4 shown.

[0027] In an exemplary embodiment of the present application, an animal microscopic imaging fixation device is provided, comprising:

[0028] Animal Bed 1,

[0029] A mouth support 2 is installed on the animal bed 1, and a lifting part for lifting the mouth of the animal is provided on the mouth support 2;

[0030] A head limiting mechanism, installed on the animal bed 1, for limiting the head of the animal;

[0031] The mounting base 3 is mounted on the animal bed 1 and is used for mounting a miniature microscope.

[0032] When in use, the mouth of the animal to be tested is placed in the mouth rest 2 to adjust the animal's posture, and then the animal's head is fixed by the head limiting mechanism, and a miniature microscope is installed in the mounting seat 3, and the interface of the miniature microscope is connected to the base plate implanted in the animal's brain.

[0033] In this embodiment, since the animal's head is fixed by the head limiting mechanism, the possibility of the animal's movement affecting the recording of the neural activity signal is reduced, and the interference of the animal's movement on the signal stability is reduced. By providing a mouth support 2, the animal's head can be straightened.

[0034] In this embodiment, the experimental animals can be mice or other animals.

[0035] Exemplarily, the shape of the portion where the mouth support 2 contacts the animal is configured to match the shape of the animal's mouth.

[0036] In an exemplary embodiment, the mounting base 3 is movably connected to the animal bed 1 , so that the mounting base 3 can rotate on the animal bed 1 .

[0037] It should be noted that, due to individual differences in the body shape of experimental animals, there may be inconsistencies in the position of the pre-embedded base plate of the animal's brain. The mounting base 3 is movably connected to the animal bed 1 to meet the needs of rapid docking and installation with the animal base plate.

[0038] In an exemplary embodiment, a central channel is provided on the mounting base 3, the animal bed 1 passes through the central channel, the mouth support 2 and the head limiting mechanism are located in the central channel, and the mounting base 3 can be rotatably mounted on the animal bed 1 with the central axis of the central channel as the rotation axis.

[0039] It should be noted that the mounting base 3 and the animal bed 1 only need to be able to rotate with the axis of the animal bed 1 as the rotation center, and in this embodiment, the mounting base 3 is provided with a central channel, and the animal bed 1 passes through the central channel to achieve the coordination between the two. The central channel is provided so that the animal bed 1 can pass through and also provides sufficient fixing space for the animal. Since the main fixing part is the head of the animal, the mouth support 2 and the head limiting mechanism are both provided in the central channel.

[0040] In an exemplary embodiment, the mounting base 3 includes an upper body 301 and a lower body 302. The animal bed 1 is located in a central channel formed by the upper body 301 and the lower body 302. The upper body 301 and the lower body 302 are detachably connected. The upper body 301 is provided with mounting holes 3011 for slidingly cooperating with the miniature microscope. The upper body 301 is also provided with a plurality of locking holes 3012 for inserting locking members to lock the miniature microscope.

[0041] In this embodiment, the upper seat 301 and the lower seat 302 are detachably connected, and the upper seat 301 can be moved in the moving groove 3023 of the lower seat 302 to switch to different installation positions, so as to facilitate the rapid disassembly and adjustment of the mounting seat 3 according to the posture and body shape of the animal. The multiple sets of locking holes 3012 are distributed along the radial direction of the central channel (or the multiple sets of locking holes 3012 are distributed in the up and down direction of the animal bed 1, and the up and down direction is Figure 1 The micro-microscope is fixed by moving the micro-limiting mirror up and down to increase the reliability of experimental data.

[0042] Exemplarily, the locking member is threadably matched with the locking hole 3012 .

[0043] Exemplarily, half holes are respectively provided on the upper body 301 and the lower body 302, and the half holes on the upper body 301 and the half holes on the lower body 302 are combined to form a central channel. The outer surfaces of the upper body 301 and the lower body 302 are semicircular arc-shaped, so that the upper body 301 and the lower body 302 are sleeve-shaped after being assembled. The mounting hole 3011 is provided in the middle of the upper body 301, and the mounting hole 3011 connects the outer side of the upper body 301 with the central channel. The micro-microscope is installed in the mounting hole 3011, and the interface of the micro-microscope extends into the central channel after passing through the mounting hole 3011, so as to dock with the base plate implanted in the animal head in the central channel.

[0044] In an exemplary embodiment, the lower seat body 302 is provided with a moving groove 3023, and the moving groove 3023 is provided with at least two moving grooves distributed in the width direction (attached) of the animal bed 1. Figure 1 The upper seat body 301 is provided with a connecting pillar 3014 for matching with the installation position.

[0045] It should also be noted that at least two mounting positions are provided to facilitate adjustment of the relative position between the animal and the miniature microscope on the mounting base 3 according to the animal's body size, etc.

[0046] In an exemplary embodiment, the movable groove 3023 is a waist-shaped hole, and the movable groove 3023 is arranged along the width direction of the animal bed 1. A blocking portion 30231 is provided at the slot position in the middle of the movable groove 3023 for preventing the connecting pillar 3014 from detaching, so that the slot width in the middle of the movable groove 3023 is smaller than the hole diameter at both ends of the movable groove 3023.

[0047] In this embodiment, a blocking portion 30231 is set in the middle of the movable groove 3023, while no blocking portion 30231 is set at the two ends of the movable groove 3023, so that the connecting pillar 3014 can enter the movable groove 3023 or escape from the movable groove 3023 from the two ends of the movable groove 3023. When the connecting pillar 3014 reaches the middle of the movable groove 3023, it will be blocked by the blocking portion 30231, so that the upper seat body 301 and the lower seat body 302 are connected.

[0048] Exemplarily, in order to increase the connection stability between the upper body 301 and the lower body 302 , the blocking portions 30231 are disposed on both sides of the moving groove 3023 .

[0049] In an exemplary embodiment, the connecting pillar 3014 includes a large diameter section 30142 and a small diameter section 30141 , the large diameter section 30142 matches with the inside of the moving groove 3023 , and the small diameter section 30141 matches with the notch in the middle of the moving groove 3023 .

[0050] It should be noted that there is a stepped transition between the large diameter section 30142 and the small diameter section 30141 , and the small diameter section 30141 is configured to cooperate with the blocking portion 30231 so that the blocking portion 30231 plays a limiting role on the connecting pillar 3014 .

[0051] In an exemplary embodiment, one of the mounting seat 3 and the animal bed 1 is provided with an arcuate slide groove 304 , and the other is provided with an arcuate slide rail 303 , and the arcuate slide rail 303 is disposed in the arcuate slide groove 304 , so that the mounting seat 3 and the animal bed 1 are slidably matched.

[0052] Exemplarily, the lower seat body 302 includes a first arc-shaped body 3021 and a second arc-shaped body 3022, the first arc-shaped body 3021 and the second arc-shaped body 3022 are arranged in parallel, the arc-shaped slide grooves 304 are distributed on the first arc-shaped body 3021 and the second arc-shaped body 3022, the arc-shaped slide rail 303 is arranged on the animal bed 1, the arc-shaped slide rail 303 is located between the first arc-shaped body 3021 and the second arc-shaped body 3022, and the arc-shaped slide rail 303 is slidably matched with the arc-shaped slide groove 304.

[0053] In an exemplary embodiment, a connecting hole 3013 for locking the upper body 301 and the microscope is opened on the upper body 301, and the connecting hole 3013 is opened along the axial direction of the connecting pillar 3014 and passes through the connecting pillar 3014. A locking connector is arranged in the connecting hole 3013, and the locking connector is threadedly matched with the connecting hole 3013. When the connecting pillar 3014 is located in the middle of the movable groove 3023 and the locking connector is pressed against the bottom of the movable groove 3023, the upper body 301 and the lower body 302 are switched from an unlocked state to a locked state. In the unlocked state, the connecting pillar 3014 can move along the movable groove 3023, and in the locked state, the connecting pillar 3014 cannot move in the movable groove 3023.

[0054] In this embodiment, the upper seat body 301 and the lower seat body 302 can be locked by a locking connector, which is convenient to use.

[0055] In an exemplary embodiment, the head limiting mechanism includes two sets of sliding components 5, and the area between the two sets of sliding components 5 is the placement area of ​​the animal's head. The sliding component 5 includes a sliding seat 501 installed on the animal bed 1 and a slider 502 installed on the sliding seat 501. After the slider 502 slides into place, it is locked and fixed on the sliding seat 501 by a locking member.

[0056] It should be noted that the size of the placement area can be changed by adjusting the position of the slider 502 on the slide seat 501, so as to facilitate the placement and fixation of the animal. When the slider 502 is adjusted to the right position on the slide seat 501, the locking member passes through the slider 502 and is threadedly connected to the slide seat 501 to fix the position of the slider 502.

[0057] In an exemplary embodiment, the head limiting mechanism further includes a limiting member 4, on which a sleeve hole 402 for limiting the jaw teeth of the animal is provided, and a channel for introducing gas is opened on the limiting member 4.

[0058] It should be noted that the setting of the sleeve hole 402 is to cover the jaw teeth of the animal, thereby effectively fixing the head of the experimental animal. The setting of the channel on the limiter 4 facilitates the introduction of anesthetic gas and oxygen. The limiter 4 can be tubular, and one end of the limiter 4 is extended into the animal bed 1 to be provided with an extension part 401, and the sleeve hole 402 is provided on the extension part 401, and the sleeve hole 402 passes through the extension part 401 from top to bottom. The shape of the extension part 401 can be adjusted to match the shape of the contact part with the animal.

[0059] Exemplarily, the animal bed 1 is provided with a mounting portion of the limit member 4, the mounting portion is provided with a through hole, and the limit member 4 is installed in the through hole, so that the limit member 4 can be moved along the length direction of the animal bed (i.e., the attachment Figure 1When the limiting member 4 moves into position, it is locked by the locking member, so that the limiting member 4 and the animal bed 1 are fixed.

[0060] In an exemplary embodiment, the animal microscopic imaging fixture further comprises a base 6, on which a first mounting protrusion 601 and a second mounting protrusion 602 for mounting an animal bed 1 are provided, the first mounting protrusion 601 is provided with a socket for inserting one end of the animal bed 1, the second mounting protrusion 602 is provided with a placement groove 6021 for placing the other end of the animal bed 1, and slots are provided on the side walls on both sides of the placement groove 6021, into which a plug block 603 for pressing and abutting against the end of the animal bed 1 is inserted.

[0061] In this embodiment, the first mounting protrusion 601 and the second mounting protrusion 602 on the base 6 are arranged so that the animal bed 1 is suspended on the base 6, so that there is an installation space and movement space for the mounting seat 3 between the base 6 and the animal bed 1. The arrangement of the socket facilitates the insertion of one end of the animal bed 1, and the arrangement of the placement groove 6021 facilitates the placement of the other end of the animal bed 1. At this time, only one end of the animal bed 1 located in the placement groove 6021 needs to be fixed to achieve the fixing of the animal bed 1 on the base 6. The arrangement of the slot 6021 facilitates the installation of the plug block 603 to achieve the compression and limiting of the end of the animal bed 1.

[0062] Exemplarily, the slot 6021 includes a transverse section and a vertical section, the transverse section and the vertical section are connected to form a right angle, and the shape of the plug block 603 matches the shape of the slot 6021. The plug block 603 is provided with a first limiting surface for pressing the animal bed 1 and a second limiting surface for abutting against the end surface of the animal bed 1 (located at one end in the placement slot).

[0063] It should also be noted that the locking members, locking members, etc. in the present application can be screws, bolts, etc.

[0064] The above embodiments are merely illustrative of the principles and effects of the present invention, and are not intended to limit the present invention. Anyone familiar with the technology may modify or alter the above embodiments without departing from the spirit and scope of the present invention. Therefore, all equivalent modifications or alterations made by a person of ordinary skill in the art without departing from the spirit and technical concept disclosed in the present invention shall still be covered by the claims of the present invention.

Claims

1. An animal microscopic imaging fixation device, characterized in that: include: Animal beds, A mouth support, mounted on the animal bed, the mouth support being provided with a lifting portion for lifting the mouth of the animal; A head limiting mechanism, installed on the animal bed, for limiting the head of the animal; A mounting base is mounted on the animal bed and is used for mounting a miniature microscope.

2. The animal microscopic imaging fixation device according to claim 1, characterized in that: The mounting seat is movably connected to the animal bed so that the mounting seat can rotate on the animal bed.

3. The animal microscopic imaging fixation device according to claim 1, characterized in that: The mounting seat is provided with a central channel, the animal bed passes through the central channel, the mouth support and the head limiting mechanism are located in the central channel, and the mounting seat can be rotatably mounted on the animal bed with the central axis of the central channel as the rotation axis.

4. The animal microscopic imaging fixation device according to claim 3, characterized in that: The mounting base includes an upper body and a lower body, the animal bed is located in the upper body and the lower body, the upper body and the lower body are detachably connected, the upper body is provided with mounting holes for slidingly cooperating with the miniature microscope, and the upper body is also provided with multiple groups of locking holes for inserting locking pieces to lock the miniature microscope.

5. The animal microscopic imaging fixation device according to claim 4, characterized in that: The lower seat body is provided with a moving groove, in which at least two installation positions distributed in the width direction of the animal bed are provided, and the upper seat body is provided with connecting pillars for matching with the installation positions.

6. The animal microscopic imaging fixation device according to claim 5, characterized in that: The movable groove is a waist-shaped hole, and the movable groove is arranged along the width direction of the animal bed. A blocking part for preventing the connection pillar from detaching is arranged at the slot position in the middle of the movable groove, so that the slot width in the middle of the movable groove is smaller than the hole diameter at both ends of the movable groove.

7. The animal microscopic imaging fixation device according to claim 6, characterized in that: The connecting pillar includes a large diameter section and a small diameter section, the large diameter section matches the inside of the moving groove, and the small diameter section matches the notch in the middle of the moving groove.

8. The animal microscopic imaging fixation device according to claim 7, characterized in that: The upper seat body is provided with a connecting hole for locking the upper seat body and the miniature microscope. The connecting hole is opened along the axial direction of the connecting pillar and passes through the connecting pillar. A locking connector is provided in the connecting hole. The locking connector is threadedly matched with the connecting hole. When the connecting pillar is located in the middle of the movable groove and the locking connector is pressed against the bottom of the movable groove, the upper seat body and the lower seat body switch from an unlocked state to a locked state.

9. The animal microscopic imaging fixation device according to any one of claims 1 to 8, characterized in that: The head limiting mechanism includes two sets of sliding components, between which is the placement area for the animal's head. The sliding component includes a sliding seat installed on the animal bed and a slider installed on the sliding seat. After the slider slides into place, it is locked and fixed on the sliding seat by a locking member.

10. The animal microscopic imaging fixation device according to claim 9, characterized in that: The head limiting mechanism also includes a limiting member, on which a sleeve hole for limiting the jaw teeth of the animal is arranged, and on which a channel for introducing gas is opened.

Images