Exosome extraction kit

Abstract

The utility model provides an exosome extraction kit which comprises a kit body, a rotating disc is arranged in the kit body, an extraction opening which is eccentrically distributed relative to the rotation center of the rotating disc is formed in the top of the kit body, a supporting sleeve is arranged on the rotating disc, a tray is arranged at the bottom of the supporting sleeve, and the rotating disc is arranged on the tray. The bottom of the tray is connected with a guide shaft which is elastically connected with the rotating disc up and down, the top of the box body is connected with a transmission shaft located on one side of the extraction opening in an up-down sliding mode, the bottom of the transmission shaft extends into the box body and is fixedly connected with a hook plate, the hook plate is used for lifting the tray, and the top of the transmission shaft is connected with a sealing cover used for blocking the extraction opening. According to the reagent box, the supporting sleeve and the rotating disc are arranged to be of an up-down elastic sliding structure, the supporting sleeve can be conveniently lifted to the extraction opening, then the transmission shaft used for driving the supporting sleeve to be lifted is arranged on the box body, the lifting operation of the supporting sleeve is more convenient, and then the convenience of reagent taking and placing is greatly improved.

Description

Technical Field

[0001] The utility model relates to the technical field of medical equipment, and specifically to an exosome extraction kit. Background Art

[0002] Exosomes are currently regarded as specifically secreted membrane vesicles that participate in intercellular communication. There is growing interest in the study of exosomes, both in studying their functions and understanding how to use them in the development of minimally invasive diagnostics. Exosome extraction kits are placement devices that need to be used during the exosome extraction process.

[0003] The utility model with the current announcement number CN217171413U proposes an exosome extraction kit, including a main box body, a mounting port is provided at one end of the top of the main box body, a transparent baffle is slidably inserted in the mounting port, one end of the transparent baffle passes through the inner wall of the mounting port and is fixedly connected with a card block, a slide groove is provided on the outer wall of the main box body, a support rod is fixedly connected in the slide groove, an L-shaped card rod is slidably sleeved on the support rod, a card groove corresponding to the L-shaped card rod is provided on the card block, a turntable is rotatably connected to the main box body through a fixed rod, a plurality of support sleeves are fixedly connected to the top edge of the turntable, a cavity corresponding to the fixed rod is provided at the bottom of the main box body, and a first gear is fixedly sleeved at one end of the fixed rod located in the cavity. In the utility model, a single reagent can be taken out through the setting of a rotating structure, effectively preventing other reagents from being exposed to the outside, thereby improving the safety of the reagent kit.

[0004] However, the above-mentioned prior art still has the following defects when used: 1. It is inconvenient to take and put a single reagent. Since the support sleeve is located in the box body, there is a certain distance between the reagent and the installation port during its movement with the turntable. This distance affects the operator's ability to take the reagent placed in the support sleeve from the installation port; 2. The sealing effect is poor. When the installation port is opened, the space inside the box body is fully connected to the outside through the installation port, and it is difficult to prevent dust from entering.

[0005] To this end, the utility model provides an exosome extraction kit. Utility Model Content

[0006] In view of the shortcomings of the prior art, the purpose of the present invention is to provide an exosome extraction kit to solve the problems raised in the above-mentioned background technology. The present invention has the advantages of being more convenient to take and place reagents, and also has the advantage that the box body is always in a sealed state to prevent dust when taking it.

[0007] In order to achieve the above-mentioned purpose, the utility model is realized through the following technical scheme: an exosome extraction kit, comprising a box body, a turntable is arranged in the box body, an extraction port eccentrically distributed relative to the rotation center of the turntable is opened on the top of the box body, a support sleeve is arranged on the turntable, a tray is arranged at the bottom of the support sleeve, a guide shaft elastically connected to the turntable up and down is connected to the bottom of the tray, a transmission shaft located on one side of the extraction port is slidably connected to the top of the box body up and down, the bottom of the transmission shaft extends into the box body and is fixedly connected to a hook plate, the hook plate is used to lift the tray, the top of the transmission shaft is connected to a cover for sealing the extraction port, and the support sleeve and the extraction port are sealed and matched.

[0008] Furthermore, a guide countersunk hole is provided on the disk surface of the turntable, and the bottom of the transmission shaft is sleeved in the guide countersunk hole and connected to the bottom of the guide countersunk hole through a tension spring.

[0009] Furthermore, a sleeve 91 is welded to the bottom of the cover, and a sealing rubber sleeve is nested in the extraction port. The sealing rubber sleeve is in a sealed matching state when plugged with the sleeve 91 and the support sleeve respectively.

[0010] Furthermore, a cantilever is welded to the outer peripheral wall of the cover, a connecting sleeve is welded to the free end of the cantilever, and the connecting sleeve is rotatably connected to the top of the transmission shaft.

[0011] Furthermore, a handle is welded on the top of the cover.

[0012] Furthermore, a guide sleeve is welded through the top of the box body, a sealing sleeve is nested in the guide sleeve, and the transmission shaft passes through the sealing sleeve.

[0013] Furthermore, an elastic contact rod is bonded to the free end of the hook plate, and the free end of the elastic contact rod is an arc groove structure.

[0014] The beneficial effects of the utility model are as follows:

[0015] 1. The utility model arranges the support sleeve and the turntable into a structure that elastically slides up and down, so that the support sleeve can be lifted to the extraction port conveniently. Then, a transmission shaft for driving the support sleeve to be lifted is arranged on the box body, so that the lifting operation of the support sleeve is more convenient, thereby greatly improving the convenience of taking and placing the reagents.

[0016] 2. The utility model provides a sealing rubber sleeve at the extraction port, the sealing rubber sleeve and the support sleeve are sealed together, and then a sealing cover is provided on the top of the transmission shaft, and a sleeve platform 91 that is sealed together with the sealing rubber sleeve is provided on the sealing cover, so that when the support sleeve needs to be lifted to take the reagent, the box body is in a sealed state at this time, preventing dust from entering the box body through the extraction port, and having the advantage of better dustproof effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 This is a schematic diagram of the structure of the turntable, rotating shaft, supporting sleeve and sealing cover of an exosome extraction kit of the utility model;

[0018] Figure 2 for Figure 1 Schematic diagram of the explosion unfolding;

[0019] Figure 3 This is a schematic diagram of the connection between a transmission shaft and a sealing cover of an exosome extraction kit of the utility model;

[0020] Figure 4 This is a cross-sectional view of an exosome extraction kit of the utility model;

[0021] In the figure: 1. box body; 11. guide sleeve; 111. sealing sleeve; 2. turntable; 21. guide countersunk hole; 3. extraction port; 31. sealing rubber sleeve; 4. support sleeve; 5. tray; 6. guide shaft; 7. transmission shaft; 8. hook plate; 9. cover; 91. sleeve; 92. cantilever; 921. connecting sleeve; 93. handle; 101. elastic touch rod; 102. tension spring. DETAILED DESCRIPTION

[0022] In order to make the technical means, creative features, objectives and effects achieved by the present invention easy to understand, the present invention is further described below in conjunction with specific implementation methods.

[0023] See also Figures 1 to 4 The utility model provides a technical solution: an exosome extraction kit, comprising a box body 1, a turntable 2 is arranged inside the box body 1, and the connection mode between the turntable 2 and the box body 1 and the structure for driving the turntable 2 to rotate are all the structures in the prior art mentioned in the background technology. An extraction port 3 eccentrically distributed relative to the rotation center of the turntable 2 is opened on the top of the box body 1, and a support sleeve 4 is arranged on the turntable 2. Through the extraction port 3, a single reagent can be docked or taken with a support sleeve 4 on the turntable 2.

[0024] In the present technical solution, a tray 5 is provided at the bottom of the support sleeve 4, and the tray 5 is welded to the support sleeve 4. The bottom of the tray 5 is connected to a guide shaft 6 elastically connected to the turntable 2 from top to bottom. Specifically, a guide countersunk hole 21 is provided on the surface of the turntable 2, and the bottom of the transmission shaft 7 is sleeved in the guide countersunk hole 21 and connected to the bottom of the guide countersunk hole 21 through a tension spring 102. The two ends of the tension spring 102 can be connected to the guide countersunk hole 21 and the bottom of the transmission shaft 7 by welding. The guide shaft 6 and the tray 5 are coaxial and welded, and the top of the box body 1 is slidably connected to the transmission shaft 7 located on one side of the extraction port 3, and the transmission shaft 7 and the box body 1 are sealed. Specifically, a guide sleeve 11 is welded through the top of the box body 1, and a sealing sleeve 111 is nested in the guide sleeve 11, and the transmission shaft 7 passes through the sealing sleeve 111. The bottom of the transmission shaft 7 extends into the box body 1 and is fixedly connected with a hook plate 8, which is used to lift the tray 5. That is to say, the height of the hook plate 8 is lower than the tray 5. When the tray 5 is located above the hook plate 8, when the transmission shaft 7 is lifted, the hook plate 8 will push the tray 5 up, thereby lifting the current support sleeve 4 out of the extraction port 3.

[0025] The top of the transmission shaft 7 is connected to a cover 9 for sealing the extraction port 3. The extraction port 3 can be made into a circle, and the support sleeve 4 and the extraction port 3 are sealed together. Specifically, a sleeve 91 is welded to the bottom of the cover 9, and a sealing rubber sleeve 31 is nested in the extraction port 3. The sealing rubber sleeve 31 is in a sealed matching state when it is plugged with the sleeve 91 and the support sleeve 4 respectively. That is to say, when the cover 9 moves downward, the sleeve 91 can be inserted into the extraction port 3, and when the support sleeve 4 moves upward, it can also be inserted into the extraction port 3. The sealing rubber sleeve 31 plays a sealing role, so that the inner cavity of the box body 1 is always in a closed environment.

[0026] In this embodiment, a cantilever 92 is welded to the outer peripheral wall of the cover 9, and a connecting sleeve 921 is welded to the free end of the cantilever 92. The connecting sleeve 921 is rotatably connected to the top of the transmission shaft 7. When the cover 9 rotates relative to the transmission shaft 7, the reagent inserted on the entire support sleeve 4 will be completely exposed, further increasing the space for taking and placing the reagent.

[0027] In this embodiment, a handle 93 is welded on the top of the cover 9, and the handle 93 is a hand-held component.

[0028] In this embodiment, an elastic touch rod 101 is bonded to the free end of the hook plate 8. The free end of the elastic touch rod 101 is a circular arc groove structure. The elastic touch rod 101 is a rubber rod, which plays a warning and limiting role. When the guide shaft 6 touches the elastic touch rod 101 and engages with the circular arc groove, the operator can sense the collision. At this time, the support sleeve 4 is in a coaxial state with the extraction port 3.

[0029] Working principle: When it is necessary to insert the reagent tube into the box body 1, the turntable 2 is controlled to rotate. When the guide shaft 6 is stuck in the arc groove at the end of the elastic touch rod 101, the handle 93 is manually held and lifted up, the hook plate 8 pushes the tray 5 up, the support sleeve 4 moves upward and squeezes into the sealing rubber sleeve 31, and the tension spring 102 is in an elongated state. At the same time, due to the damping effect of the sealing rubber sleeve 31, the support sleeve 4 is in a stationary state. The reagent tube with exosomes is inserted into the support sleeve 4, and then the cover 9 is pressed downward. The sleeve 91 moves downward and pushes the support sleeve 4 into the box body 1. At the same time, the sleeve 91 will be inserted into the sealing rubber sleeve 31, and the operation of placing a single reagent into the box body 1 is realized. When a single reagent needs to be taken, according to the above operation method, when the handle 93 is lifted, the sleeve 91 does not leave the sealing rubber sleeve 31, and the support sleeve 4 will enter the sealing rubber sleeve 31. At this time, the box body 1 is always in a closed state, which can effectively prevent dust from entering through the extraction port 3.

[0030] In addition, it should be understood that although the present specification is described according to implementation modes, not every implementation mode contains only one independent technical solution. This description of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each embodiment may also be appropriately combined to form other implementation modes that can be understood by those skilled in the art.

Claims

1. An exosome extraction kit, comprising a box body (1), wherein a turntable (2) is arranged inside the box body (1), an extraction port (3) eccentrically distributed relative to the rotation center of the turntable (2) is opened on the top of the box body (1), and a support sleeve (4) is arranged on the turntable (2), characterized in that: A tray (5) is arranged at the bottom of the support sleeve (4), and the bottom of the tray (5) is connected to a guide shaft (6) elastically connected to the turntable (2) up and down. The top of the box body (1) is slidably connected to a transmission shaft (7) located on one side of the extraction port (3). The bottom of the transmission shaft (7) extends into the box body (1) and is fixedly connected to a hook plate (8), which is used to lift the tray (5). The top of the transmission shaft (7) is connected to a cover (9) for sealing the extraction port (3). The support sleeve (4) and the extraction port (3) are sealed and matched.

2. An exosome extraction kit according to claim 1, characterized in that: A guide countersunk hole (21) is provided on the surface of the rotating disk (2), and the bottom of the transmission shaft (7) is sleeved in the guide countersunk hole (21) and connected to the bottom of the guide countersunk hole (21) via a tension spring (102).

3. An exosome extraction kit according to claim 1, characterized in that: A sleeve 91 (91) is welded to the bottom of the sealing cover (9), and a sealing rubber sleeve (31) is nested in the extraction port (3). The sealing rubber sleeve (31) is in a sealed matching state when plugged into the sleeve 91 (91) and the support sleeve (4) respectively.

4. An exosome extraction kit according to claim 3, characterized in that: A cantilever (92) is welded to the outer peripheral wall of the sealing cover (9), a connecting sleeve (921) is welded to the free end of the cantilever (92), and the connecting sleeve (921) is rotatably connected to the top of the transmission shaft (7).

5. The exosome extraction kit according to claim 1, characterized in that: A handle (93) is welded to the top of the cover (9).

6. An exosome extraction kit according to claim 1, characterized in that: A guide sleeve (11) is welded through the top of the box body (1), a sealing sleeve (111) is nested in the guide sleeve (11), and the transmission shaft (7) passes through the sealing sleeve (111).

7. An exosome extraction kit according to claim 1, characterized in that: An elastic contact rod (101) is bonded to the free end of the hook plate (8), and the free end of the elastic contact rod (101) is a circular arc groove structure.

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