Observation device for stem cell culture

By designing a stem cell culture observation device including a sealed chamber and observation components, the difficulties of culturing and observing multiple sets of stem cell cultures and observation in the prior art are solved, and efficient use of the equipment and improvement of observation effect are achieved.

CN222556953UActive Publication Date: 2025-03-04JIANGSU HEZE STEM CELL GENE ENG CO LTD
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Patent Information

Application Number
CN202421024686.3
Authority / Receiving Office
CN · China
Patent Type
Utility models(China)
Current Assignee / Owner
Filing Date
2024-05-13
Publication Date
2025-03-04
Estimated Expiration
2034-05-13

AI Technical Summary

Technical Problem

It is difficult to conduct centralized cultivation and observation of multiple groups of stem cells at the same time, and the equipment is not convenient for stem cells to be taken out, which affects the efficiency of use and observation effect.

Method used

An observation device including a sealing chamber and observation assembly is designed. The sealing chamber is equipped with a support plate, a support column, a placing plate and a Petri dish. The sealing and convenient removal are achieved through a hinged sealing door and a moving groove. Combined with the clamping structure of the elastic cylinder and the clamping block, it is suitable for Petri dishes of different diameters.

Benefits of technology

The simultaneous culture and observation of multiple groups of stem cells is realized, and the rapid removal of stem cells inside the equipment and multiple observations outside the equipment have improved the working efficiency and effect of the equipment.

✦ Generated by Eureka AI based on patent content.

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Abstract

The utility model discloses an observation device for stem cell culture, which comprises a sealed chamber and an observation assembly, the observation assembly comprises a support disc arranged in the sealed chamber, a support column is fixed on the support disc, a placing disc is sleeved and rotatably arranged on the support column, a culture dish is arranged on the placing disc, and the culture dish is provided with a culture cavity. The bottom of the inner side of the sealing chamber and the surface of the sealing door are each provided with a moving groove, and the moving strips are arranged in the moving grooves in a sliding mode. Through cooperation between the sealing chamber and the sealing door, the whole device can be sealed, and therefore stem cells can be cultured and observed in the device; meanwhile, by installing the observation assembly, the equipment can observe multiple groups of stem cells at the same time, and the observation assembly can be quickly taken out from the interior of the sealing chamber, so that the equipment can be conveniently and quickly observed for multiple times through external observation equipment, and the working efficiency and the working effect of the equipment can be improved.
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Description

Technical Field

[0001] The invention relates to the technical field of observation devices, and more specifically, to an observation device for stem cell culture. Background Art

[0002] Stem cells are a type of cell that has unlimited or immortal self-renewal capabilities and can produce at least one type of highly differentiated daughter cells. When conducting experiments on stem cells, the stem cells need to be cultured and observed in a centralized manner, so a stem cell culture observation device is needed.

[0003] Among the currently used stem cell culture observation devices, the equipment is not convenient for centralized culture and observation of multiple groups of stem cells, which affects the efficiency of the equipment. At the same time, during the stem cell culture process, the equipment is not convenient for taking out stem cells, which affects the effect of the equipment in observing stem cells.

[0004] Therefore, it is necessary to provide an observation device for stem cell culture to solve the above problems. Utility Model Content

[0005] Based on the above problems existing in the prior art, the purpose of the embodiments of the present application is to provide an observation device for stem cell culture to achieve the effect of culturing and observing multiple groups of stem cells.

[0006] The technical solution adopted by the present application to solve its technical problems is: an observation device for stem cell culture, including a sealed chamber and an observation component, the observation component including a support plate arranged in the sealed chamber, a support column fixed on the support plate, a placement plate sleeved and rotatably arranged on the support column, a culture dish arranged on the placement plate, a sealed door hinged on one side of the sealed chamber, a movable groove is provided on the inner bottom of the sealed chamber and the surface of the sealed door, a movable bar is fixedly connected to the bottom of the support plate, and the movable bar is slidably arranged in the movable groove.

[0007] Furthermore, an annular groove is provided on the top of the support plate, the axis of the annular groove is colinear with the rotation axis of the placement plate, and a plurality of support bars are distributed on the axis of the bottom of the placement plate, and the support bars are inserted into the annular groove.

[0008] Furthermore, the placement plate includes a plate body, a fixing box is fixedly connected to the top of the plate body, an elastic tube is installed inside the fixing box, and a clamping block is fixedly connected to one side of the elastic tube.

[0009] Furthermore, the top of the support plate is fixedly connected to a support column, the outer side of the bottom of the support column is fixedly connected to a support base, a second fixed block is installed on one side of the top of the support column, the top of the support column is movably connected to multiple sets of moving wheels, and a third fixed block is installed on the top of the support plate.

[0010] Furthermore, the sealing chamber is made of a transparent material, the first fixed block and the end of the sealing door are matched with each other, the interior of the movable groove and the movable bar are matched with each other, and a plurality of transmission wheels are installed at the bottom of the movable bar.

[0011] Furthermore, an elastic member is installed inside the elastic tube, the clamping block is an arc-shaped structure, and the number of the clamping blocks is four groups.

[0012] 1. The utility model is equipped with a sealed chamber and a sealed door so that the whole device can be sealed, so that stem cells can be cultured and observed inside the device. At the same time, by installing an observation component, the device can observe multiple groups of stem cells at the same time, and the observation component can be quickly taken out from the inside of the sealed chamber, so that the device can be easily observed multiple times by external observation equipment, so as to improve the working efficiency and working effect of the device.

[0013] 2. The utility model enables the culture dish to be clamped after being placed inside the fixing box through the cooperation between the clamping block and the elastic tube, thereby ensuring the stability of the culture dish. At the same time, the fixing box can be suitable for culture dishes of different diameters, so as to improve the use effect of the equipment. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] The drawings constituting part of the present invention are used to provide a further understanding of the present invention. The exemplary embodiments of the present invention and their descriptions are used to explain the present invention and do not constitute an improper limitation on the present invention. In the drawings:

[0015] Figure 1 This is a schematic diagram of the structure of an observation device for stem cell culture according to the utility model;

[0016] Figure 2 This is a schematic diagram of the installation structure of the observation assembly of the utility model;

[0017] Figure 3 This is a schematic diagram of the installation structure of the placement tray of the utility model;

[0018] Figure 4 This is a schematic diagram of the installation structure of the movable bar of the utility model;

[0019] Figure 5 This is a schematic diagram of the mobile wheel installation structure of the utility model;

[0020] In the figure: 1. Sealed chamber; 101. Sealed door; 102. Fixing hole; 103. First fixed block; 104. Moving groove; 2. Observation assembly; 201. Support plate; 202. Moving bar; 203. Annular groove; 204. Support bar; 205. Placement plate; 2051. Plate body; 2052. Fixing box; 2053. Clamping block; 2054. Elastic cylinder; 206. Culture dish; 207. Support column; 208. Support base; 209. Second fixed block; 210. Moving wheel; 211. Third fixed block. DETAILED DESCRIPTION

[0021] It should be noted that, in the absence of conflict, the embodiments and features in the embodiments of the present application can be combined with each other. The present invention will be described in detail below with reference to the accompanying drawings and in combination with the embodiments.

[0022] It should be noted that, unless otherwise specified, all technical and scientific terms used in this application have the same meanings as commonly understood by ordinary technicians in the technical field to which this application belongs.

[0023] In the present invention, unless otherwise specified, directions such as "up" and "down" are usually relative to the directions shown in the drawings, or relative to the vertical, perpendicular or gravity direction; similarly, for ease of understanding and description, "left" and "right" are usually relative to the left and right shown in the drawings; "inside" and "outside" refer to the inside and outside relative to the outline of each component itself, but the above-mentioned directions are not used to limit the present invention.

[0024] like Figure 1-5 As shown, the present application provides an observation device for stem cell culture, including a sealed chamber 1 and an observation component 2, the observation component 2 includes a support plate 201 arranged in the sealed chamber 1, a support column 207 is fixed on the support plate 201, a placement plate 205 is sleeved and rotatably arranged on the support column 207, a culture dish 206 is arranged on the placement plate 205, a sealed door 101 is hinged on one side of the sealed chamber 1, a movable groove 104 is provided on the inner bottom of the sealed chamber 1 and the surface of the sealed door 101, a movable bar 202 is fixedly connected to the bottom of the support plate 201, and the movable bar 202 is slidably arranged in the movable groove 104.

[0025] When the device is in use, the extracted stem cells are placed in the culture dish 206, and the sealing door 101 is passed through the first fixing block 103, so that the entire sealed chamber 1 is sealed, so that the stem cells can be cultured in a warm state, and the sealed chamber 1 is a transparent structure, so that the staff can observe the inside of the device in a sealed state;

[0026] After the sealed door 101 is opened, it will be aligned with the position of the sealed chamber 1, so that the sealed door 101 and the inner bottom of the sealed chamber 1 will be in a flat state. At this time, the entire observation component 2 can be pulled outward, so that the observation component 2 can be taken out from the inside of the sealed chamber 1, so that the staff can conveniently observe the stem cells inside the observation component 2 from the outside, thereby improving the use effect of the equipment and the observation diversity;

[0027] When the observation component 2 is moved, the second fixing block 209 can be loosened to separate the top of the support column 207 from the top of the sealed chamber 1, and then the observation component 2 can be pulled as a whole inside the sealed chamber 1, so that the observation component 2 can be taken out. During the pulling process, the third fixing block 211 will be close to the fixing hole 102, and will enter the sealing door 101 after the observation component 2 moves. When it moves to the extreme position, the third fixing block 211 and the fixing hole 102 will be combined, so that the observation component 2 will be fixed after it is taken out, thereby avoiding shaking when the observation component 2 is observed. Furthermore, when the observation component 2 moves, the two sets of moving bars 202 move at the moving groove 104, so that the smoothness of the observation component 2 can be improved when it moves.

[0028] An annular groove 203 is provided on the top of the support plate 201, and the axis of the annular groove 203 is colinear with the rotation axis of the placement plate 205. A plurality of support bars 204 are distributed along the axis of the bottom of the placement plate 205, and the support bars 204 are inserted into the annular groove 203. The placement plate 205 includes a plate body 2051, and a fixing box 2052 is fixedly connected to the top of the plate body 2051. An elastic tube 2054 is installed inside the fixing box 2052, and a clamping block 2053 is fixedly connected to one side of the elastic tube 2054.

[0029] When the placement tray 205 is in use, the tray body 2051 contacts the top of the support base 208, so that the entire placement tray 205 can be placed. At the same time, the support column 207 allows the placement tray 205 itself to rotate, so that the four groups of culture dishes 206 can be rotated and observed at the same position of the device, thereby improving the observation efficiency. Furthermore, the culture dishes 206 allow stem cells to be stored and cultured. At the same time, when an external device observes one group of culture dishes 206, the tray body 2051 can be rotated, and one group of culture dishes 206 will be aligned with the external observation device, thereby achieving observation outside the device.

[0030] A second fixed block 209 is installed on one side of the top of the support column 207, and a plurality of moving wheels 210 are movably connected to the top of the support column 207. A third fixed block 211 is installed on the top of the support plate 201. The sealed chamber 1 is made of transparent material, the first fixed block 103 and the end of the sealed door 101 are matched with each other, the interior of the moving groove 104 and the moving bar 202 are matched with each other, and a plurality of transmission wheels are installed at the bottom of the moving bar 202.

[0031] The elastic tube 2054 is internally installed with an elastic member, and the clamping block 2053 is an arc-shaped structure. The number of the clamping blocks 2053 is four groups. By installing the clamping blocks 2053 and the elastic tube 2054, the culture dish 206 can be squeezed when it is installed inside the fixing box 2052. On the one hand, the culture dish 206 can be clamped after being placed inside the fixing box 2052, thereby ensuring the stability of the culture dish 206; on the other hand, the fixing box 2052 can be suitable for culture dishes 206 of different diameters, so as to improve the use effect of the device.

[0032] The second fixing block 209 can be in contact with the inner top of the sealed chamber 1, so that the observation component 2 can be fixed when installed inside the sealed chamber 1, wherein the second fixing block 209 and the first fixing block 103 are simultaneously installed with fixing pins, and the inner top of the sealed chamber 1 is installed through the fixing pins inside the second fixing block 209, so that the stability of the observation component 2 during installation can be improved, and at the same time, the moving wheel 210 can contact with the inner top of the sealed chamber 1, so that the smoothness of the observation component 2 when taking it out can be further improved, and when the placement plate 205 rotates on the top of the support base 208, the four groups of support bars 204 contact the position of the annular groove 203, so that the stability can be improved when the placement plate 205 is rotated and adjusted.

[0033] A plurality of transmission wheels are installed at the bottom of the movable bar 202. The sealed chamber 1 is made of transparent material, so that the internal of the device can be observed in a sealed state. At the same time, the first fixed block 103 is adapted to the end of the sealed door 101, so that the sealed door 101 can be fixedly combined with the sealed chamber 1.

[0034] The bottom of the placement tray 205 is in contact with the support base 208 , and the number of support bars 204 is four groups. The bottom of the placement tray 205 is in contact with the bottom of the support base 208 , so that the placement tray 205 can be installed on the outside of the support column 207 .

[0035] An elastic part is installed inside the elastic tube 2054, and the clamping block 2053 is an arc-shaped structure. The number of the clamping blocks 2053 is four. The elastic part is installed inside the elastic tube 2054, so that the elastic tube 2054 has elasticity, so that the clamping block 2053 can clamp the culture dish 206 through the elastic tube 2054. The clamping block 2053 is an arc-shaped structure, so that the clamping block 2053 and the culture dish 206 are more adaptable.

[0036] Obviously, the above-described embodiments are only a part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work should fall within the scope of protection of the present invention.

[0037] It should be noted that the terms used herein are only for describing specific embodiments and are not intended to limit the exemplary embodiments according to the present application. As used herein, unless the context clearly indicates otherwise, the singular form is also intended to include the plural form. In addition, it should be understood that when the terms "comprise" and / or "include" are used in this specification, it indicates the presence of features, steps, operations, devices, components and / or combinations thereof.

[0038] It should be noted that the terms "first", "second", etc. in the specification and claims of the present application and the above-mentioned drawings are used to distinguish similar objects, and are not necessarily used to describe a specific order or sequence. It should be understood that the numbers used in this way can be interchanged where appropriate, so that the embodiments of the present application described herein can be implemented in an order other than those illustrated or described herein.

[0039] The above description is only the preferred embodiment of the present invention and is not intended to limit the present invention. For those skilled in the art, the present invention may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

[0040] The above is only a preferred embodiment of the present invention, and the protection scope of the present invention is not limited to the above embodiments. All technical solutions under the concept of the present invention belong to the protection scope of the present invention. It should be pointed out that for ordinary technicians in this technical field, some improvements and modifications without departing from the principle of the present invention should also be regarded as the protection scope of the present invention.

Claims

1. An observation device for stem cell culture, comprising a sealed chamber (1), an observation assembly (2), and a first fixing block (103), characterized in that: The observation assembly (2) comprises a support plate (201) arranged in a sealed chamber (1), a support column (207) being fixed on the support plate (201), a placement plate (205) being sleeved and rotatably arranged on the support column (207), a culture dish (206) being arranged on the placement plate (205), a sealed door (101) being hingedly connected to one side of the sealed chamber (1), a movable groove (104) being provided on the inner bottom of the sealed chamber (1) and the surface of the sealed door (101), a movable bar (202) being fixedly connected to the bottom of the support plate (201), and the movable bar (202) being slidably arranged in the movable groove (104).

2. The observation device for stem cell culture according to claim 1, characterized in that: The top of the support plate (201) is provided with an annular groove (203), the axis of the annular groove (203) is colinear with the rotation axis of the placement plate (205), and a plurality of support bars (204) are distributed along the bottom axis of the placement plate (205), and the support bars (204) are inserted into the annular groove (203).

3. The observation device for stem cell culture according to claim 1, characterized in that: The placement tray (205) comprises a tray body (2051), the top of the tray body (2051) is fixedly connected to a fixing box (2052), an elastic tube (2054) is installed inside the fixing box (2052), and a clamping block (2053) is fixedly connected to one side of the elastic tube (2054).

4. The observation device for stem cell culture according to claim 1, characterized in that: The top of the support plate (201) is fixedly connected to a support column (207), the outer side of the bottom of the support column (207) is fixedly connected to a support base (208), a second fixed block (209) is installed on one side of the top of the support column (207), the top of the support column (207) is movably connected to multiple groups of moving wheels (210), and a third fixed block (211) is installed on the top of the support plate (201).

5. The observation device for stem cell culture according to claim 1, characterized in that: The sealing chamber (1) is made of a transparent material, the first fixed block (103) and the end of the sealing door (101) are mutually matched, the interior of the moving groove (104) and the moving bar (202) are mutually matched, and a plurality of transmission wheels are installed at the bottom of the moving bar (202).

6. The observation device for stem cell culture according to claim 3, characterized in that: An elastic member is installed inside the elastic tube (2054), the clamping blocks (2053) are of arc-shaped structure, and the number of the clamping blocks (2053) is four groups.